CN103400529B - A kind of whole-body muscle Slide processing of mature rooster - Google Patents
A kind of whole-body muscle Slide processing of mature rooster Download PDFInfo
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- CN103400529B CN103400529B CN201310287046.1A CN201310287046A CN103400529B CN 103400529 B CN103400529 B CN 103400529B CN 201310287046 A CN201310287046 A CN 201310287046A CN 103400529 B CN103400529 B CN 103400529B
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Abstract
The whole-body muscle Slide processing of a kind of mature rooster, selects anesthesia, locates the corpse of after death time mature rooster within five minutes；Then to cock quick filling 1000ml normal saline, perfusion is carried out with the Aqueous Solutions of Polyethylene Glycol Aqueous Solutions of Polyethylene Glycol that mass percent is 20%, 40%, 60%, 80% and 90% the most successively；Then cock corpse is carried out peeling, and completely isolates cock whole-body muscle；After cock whole-body muscle uses the dehydrated alcohol of 1:1 and the mixed solution of dimethylbenzene carry out defat, dry in the air 5～7 days in dark ventilation, it is 3%～5% detergent immersion 1～2h by the ram nerve mass percent dried well again, then clean water is used three times, then with after the hydrogen peroxide bleaching after dilution cleaning, fixing on a glass with glue, when making cock whole-body muscle keep ram to stand posture, put in preparation jar, i.e. prepare the whole-body muscle specimen of mature rooster；Preparation method of the present invention is simple, with low cost, the whole-body muscle specimen of prepared mature rooster, it is easy to preserving, structural integrity is attractive in appearance, and morphologic localization understands nature.
Description
Technical field
The present invention relates to a kind of Slide processing, the whole-body muscle Slide processing of a kind of mature rooster.
Background technology
Zoological specimens, be to maintain former state in kind or through arrangement be available for study, research time referential can be as the individuality represented in one species, it is possible to as the important references of the universal publicity of scientific research, teaching and Sci-tech Knowledge.Scientific value, ornamental value and the reserve value that what animal and tissue specimen contained can not be substituted, other arts work of erect image equally enter into family, become the indoor new ornament of people.One makes exquisite specimen and not only brings people's knowledge, the most also brings people the enjoyment of art.Perfect animal muscle specimen is a vivid practical teaching aid, can be again a consummate art work, and it represents colleges and universities, the level of development of a subject and grasps the professional skill of this disciplinary study person.Preparation of specimen is also a research work, in manufacturing process, can constantly discover problems and solve them, and creates new technology, can broaden one's knowledge simultaneously, tempers thinking and the manipulative ability of participant.It is fabricated to whole muscle specimen, whole-body muscle structure can be demonstrated, specimen structure is attractive in appearance, form understands nature, not only as directly perceived, vivid teaching aid in " animal anatomy " imparts knowledge to students, and student can be allowed in manufacturing process to participate in, allow student understand and grasp preparation of specimen's process, simultaneously the starting of training student, beat one's brains ability.Contribute to, to student's understanding to animal whole-body muscle overview, the contour structures of whole-body muscle being grasped, the understanding of the Name & Location relation of muscle is all played well help, is favorably improved teaching efficiency.
Summary of the invention
The present invention seeks to as solving above-mentioned technical problem, it is provided that the whole-body muscle Slide processing of a kind of mature rooster, its preparation method is simple, and prepared specimen can demonstrate whole-body muscle structure, and structure is attractive in appearance, and form understands nature.
A kind of whole-body muscle Slide processing of mature rooster, its concrete preparation process is:
Step one, solution are prepared: with hydrogen peroxide is diluted to the solution that mass percent is 3.5% of hydrogen peroxide with distilled water, prepare the hydrogen peroxide after dilution, standby；Weigh detergent, add distilled water, prepare the detergent solution that mass percent is 3%～5%；Weigh the Macrogol 600 of Different Weight respectively, add distilled water and prepare the Aqueous Solutions of Polyethylene Glycol that mass percent is 20%, 40%, 60%, 80% and 90% respectively；Prepared solution is wanted matching while using or is placed in 4 DEG C of preservations；
Step 2, the corpse of selection mature rooster；
Step 3, at the corpse abdominal cavity skin of cock, open the mouth of a diameter of 0.8 ~ 1.2 cm with scalpel, expose heart；On right auricle, the osculum of a diameter of 0.3 ~ 0.4 cm is cut with operating scissors, perfusion syringe needle is inserted in the left ventricle apex of the heart, fix with mosquito forceps, quick filling 1000 ml normal saline, after blood is rinsed well, the most successively pouring quality percentage ratio be 20%, 40%, 60%, 80% and each 800ml of Aqueous Solutions of Polyethylene Glycol of 90%, first quick and back slow, the most about 2 hours；
Step 4, peeling: faced upward by cock corpse and be put on dissecting table, cut the osculum of a diameter of 0.5 ~ 1cm inside tarsal joint with operating scissors, then cuts off cock skin inside cut osculum to anus front；It is then peeled off cock skin, by the method for drill drift, skin is sloughed to cervical region so that it is become dermatotome housing type outwardly；Cut off the auricular cartilage of ear again, separate eye and rostral surrounding skin, peel off skin with caution at the apex of the wing, skin is completely stripped, finally gill；
Step 5, separation cock whole-body muscle: use scalpel to isolate the whole-body muscle of cock；Concrete operation method is: peels off and isolates scalp flesh, musculus cutaneus colli and trunk musculus cutaneus under head, cervical region and body skin after superficial fascia.Wipe out fascia and connective tissue along muscle Germ distribution, isolate the facial muscle of head, masticatory muscles and pharyngeal flesh；At cervical region separation cervical muscle, musculus hyoideus, multiple flesh and digastric.At trunk, after boundary, terminal stripping fascia, connective tissue, blood vessel and the muscle of muscle, separate the muscle such as flesh on Intercostal muscle, levatores costarum, scalenus, longus colli, omoplate (flesh after shoulder arm), latissimus dorsi m., Intercostal muscle, ilium tibialis anterior (sartorius m.), gluteus medius, tail elevator, musculus depressor coccygis, ilium shin musculus lateralis externi and ilium calf flesh (biceps femoris), musculus pronator profundus, Ulnar Wrist musculus flexor, arm triceps muscle, arm biceps, musculus pronator sublimis, palm oar side extensor, ala longue, palm oar side extensor, musculus extensor digitorum, palm side extensor and outside upper ankle cubitalis.In drumsticks portion, separate musculus flexor and gracilis etc. inside ilium shin musculus lateralis externi, ilium calf flesh, thigh lateral musculus flexor, ilium tibialis anterior, pectineus, adductor, toe；
Step 6, defat: by isolated cock muscle, rinse well with water, be placed in leaching container, be subsequently adding the ethanol water that mass percent is 80%, makes ethanol water not have cock muscle completely, soaks two days later, and taking-up water is rinsed well；Again put back to leaching container, add dehydrated alcohol and the mixed solution of dimethylbenzene that volume ratio is 1:1, dehydrated alcohol and dimethylbenzene mixed solution is made not to have cock muscle completely, changed every 12 ~ 24 hours and once soak the dehydrated alcohol and dimethylbenzene mixed solution that volume ratio used is 1:1, soak and take out two days later；Or isolated cock muscle is placed in leaching container, pours gasoline into, airtight, soak and carry out defat in about 7 days.During degreasing operation, obturage leaching container with Polypropylence Sheet, prevent solution evaporation, in order to avoid operator are damaged；
Process after step 7, defat: the cock muscle after defat is placed on dark ventilation and dries in the air 5～7 days, then be that 3%～5% detergent solution soaks 1～2h by the cock muscle mass percent dried well, then use clean water three times；
Step 8, above-mentioned steps seven is processed after cock muscle insert the hydrogen peroxide after dilution, take out after soaking 2～5 days, rinse well with clear water, dry；
Step 9, with glue by fixing for muscle on a glass, posture when making muscle keep cock to stand, be then placed in preparation jar, i.e. prepare the whole-body muscle specimen of mature rooster.
Provide the benefit that:
1, the whole-body muscle Slide processing of mature rooster of the present invention, preparation method is simple, easy, with low cost；Using ethanol water, dehydrated alcohol and dimethylbenzene or gasoline that cock muscle has been carried out ungrease treatment, defat is thorough, can prevent from using permanent and preserving, slowly oozing out of cock muscle oils and fats, pollute mouldy, make specimen impaired, hinders use and attractive in appearance.After using the dilution that mass percent is 3.5% of hydrogen oxide, hydrogen peroxide is bleached, and effect is preferably and to cock whole-body muscle specimen not damaged.
2, the whole-body muscle Slide processing of mature rooster of the present invention, the whole-body muscle specimen of prepared cock, it is easy to preserve, structural integrity is attractive in appearance, morphologic localization understands that nature, whole-body muscle are all clearly illustrated, each piece of muscle represents the most well and is fixed on the natural place of chicken body.Solve and be difficult to the clear problem differentiating cock whole-body muscle.Muscle is the emphasis in Teaching of Anatomy, the mainly theory of the muscle in systematic anatomy teaching, and topologic Students in Teaching does not dissect whole muscle；The present invention preferably resolves this difficult problem.Excite again student learning interest, improve teaching efficiency.
Detailed description of the invention
A kind of whole-body muscle Slide processing of mature rooster, its concrete preparation process is:
Step one, solution are prepared: hydrogen peroxide is diluted to distilled water the solution that mass percent is 3.5% of hydrogen peroxide, prepares the hydrogen peroxide after dilution, standby；Weigh detergent, add distilled water, prepare the detergent solution that mass percent is 3%～5%；Weigh the Macrogol 600 of Different Weight respectively, add distilled water and prepare the Aqueous Solutions of Polyethylene Glycol that mass percent is 20%, 40%, 60%, 80% and 90% respectively；Prepared solution is wanted matching while using or is placed in 4 DEG C of preservations；
Step 2, the corpse of selection mature rooster；
Step 3, at the corpse abdominal cavity skin of cock, open the mouth of a diameter of 0.8 ~ 1.2 cm with scalpel, expose heart；On right auricle, the osculum of a diameter of 0.3 ~ 0.4 cm is cut with operating scissors, perfusion syringe needle is inserted in the left ventricle apex of the heart, fix with mosquito forceps, quick filling 1000 ml normal saline, after blood is rinsed well, the most successively pouring quality percentage ratio be 20%, 40%, 60%, 80% and each 800ml of Aqueous Solutions of Polyethylene Glycol of 90%, first quick and back slow, the most about 2 hours；
Step 4, peeling: faced upward by cock corpse and be put on dissecting table, cut the osculum of a diameter of 0.5 ~ 1cm inside tarsal joint with operating scissors, then cuts off cock skin inside cut osculum to anus front；It is then peeled off cock skin, by the method for drill drift, skin is sloughed to cervical region so that it is become dermatotome housing type outwardly；Cut off the auricular cartilage of ear again, separate eye and rostral surrounding skin, peel off skin with caution at the apex of the wing, skin is completely stripped, finally gill；
Step 5, separation cock whole-body muscle: use scalpel to isolate the whole-body muscle of cock；Concrete operation method is: peels off and isolates scalp flesh, musculus cutaneus colli and trunk musculus cutaneus under head, cervical region and body skin after superficial fascia.Wipe out fascia and connective tissue along muscle Germ distribution, isolate the facial muscle of head, masticatory muscles and pharyngeal flesh；At cervical region separation cervical muscle, musculus hyoideus, multiple flesh and digastric.At trunk, after boundary, terminal stripping fascia, connective tissue, blood vessel and the muscle of muscle, separate the muscle such as flesh on Intercostal muscle, levatores costarum, scalenus, longus colli, omoplate (flesh after shoulder arm), latissimus dorsi m., Intercostal muscle, ilium tibialis anterior (sartorius m.), gluteus medius, tail elevator, musculus depressor coccygis, ilium shin musculus lateralis externi and ilium calf flesh (biceps femoris), musculus pronator profundus, Ulnar Wrist musculus flexor, arm triceps muscle, arm biceps, musculus pronator sublimis, palm oar side extensor, ala longue, palm oar side extensor, musculus extensor digitorum, palm side extensor and outside upper ankle cubitalis.In drumsticks portion, separate musculus flexor and gracilis etc. inside ilium shin musculus lateralis externi, ilium calf flesh, thigh lateral musculus flexor, ilium tibialis anterior, pectineus, adductor, toe；
Step 6, defat: by isolated cock muscle, rinse well with water, be placed in leaching container, be subsequently adding the ethanol water that mass percent is 80%, makes ethanol water not have cock muscle completely, soaks two days later, and taking-up water is rinsed well；Again put back to leaching container, add dehydrated alcohol and the mixed solution of dimethylbenzene that volume ratio is 1:1, dehydrated alcohol and dimethylbenzene mixed solution is made not to have cock muscle completely, changed every 12 ~ 24 hours and once soak the dehydrated alcohol and dimethylbenzene mixed solution that volume ratio used is 1:1, soak and take out two days later；Or isolated cock muscle is placed in leaching container, pours gasoline into, airtight, soak and carry out defat in about 7 days.During degreasing operation, obturage leaching container with Polypropylence Sheet, prevent solution evaporation, in order to avoid operator are damaged；
Process after step 7, defat: the cock muscle after defat is placed on dark ventilation and dries in the air 5～7 days, then be that 3%～5% detergent solution soaks 1～2h by the cock muscle mass percent dried well, then use clean water three times；
Step 8, above-mentioned steps seven is processed after cock muscle insert the hydrogen peroxide after dilution, take out after soaking 2～5 days, rinse well with clear water, dry；
Step 9, with glue by fixing for muscle on a glass, posture when making muscle keep cock to stand, be then placed in preparation jar, i.e. prepare the whole-body muscle specimen of mature rooster.
Following example can make those skilled in the art understand the present invention comprehensively, but limits the present invention never in any form.
Embodiment
1
A kind of whole-body muscle Slide processing of mature rooster, its concrete preparation process is:
Step one, the corpse of selection mature rooster；
Step 2, at the corpse abdominal cavity skin of cock, open the mouth of a diameter of 0.8 cm with scalpel, expose heart；On right auricle, the osculum of a diameter of 0.4 cm is cut with operating scissors, perfusion syringe needle is inserted in the left ventricle apex of the heart, fix with mosquito forceps, quick filling 1000 ml normal saline, after blood is rinsed well, the most successively pouring quality percentage ratio be 20%, 40%, 60%, 80% and each 800ml of Aqueous Solutions of Polyethylene Glycol of 90%, first quick and back slow, the most about 2 hours；
Step 3, peeling: faced upward by cock corpse and be put on dissecting table, cut the osculum of a diameter of 1cm inside tarsal joint with operating scissors, then cuts off cock skin inside cut osculum to anus front；It is then peeled off cock skin, by the method for drill drift, skin is sloughed to cervical region so that it is become dermatotome housing type outwardly；Cut off the auricular cartilage of ear again, separate eye and rostral surrounding skin, peel off skin with caution at the apex of the wing, skin is completely stripped, finally gill；
Step 4, separation cock whole-body muscle: use scalpel to isolate the whole-body muscle of cock；Concrete operation method is: peels off and isolates scalp flesh, musculus cutaneus colli and trunk musculus cutaneus under head, cervical region and body skin after superficial fascia.Wipe out fascia and connective tissue along muscle Germ distribution, isolate the facial muscle of head, masticatory muscles and pharyngeal flesh；At cervical region separation cervical muscle, musculus hyoideus, multiple flesh and digastric.At trunk, after boundary, terminal stripping fascia, connective tissue, blood vessel and the muscle of muscle, separate the muscle such as flesh on Intercostal muscle, levatores costarum, scalenus, longus colli, omoplate (flesh after shoulder arm), latissimus dorsi m., Intercostal muscle, ilium tibialis anterior (sartorius m.), gluteus medius, tail elevator, musculus depressor coccygis, ilium shin musculus lateralis externi and ilium calf flesh (biceps femoris), musculus pronator profundus, Ulnar Wrist musculus flexor, arm triceps muscle, arm biceps, musculus pronator sublimis, palm oar side extensor, ala longue, palm oar side extensor, musculus extensor digitorum, palm side extensor and outside upper ankle cubitalis.In drumsticks portion, separate musculus flexor and gracilis etc. inside ilium shin musculus lateralis externi, ilium calf flesh, thigh lateral musculus flexor, ilium tibialis anterior, pectineus, adductor, toe；
Step 5, defat: by isolated cock muscle, rinse well with water, be placed in leaching container, be subsequently adding the ethanol water that mass percent is 80%, makes ethanol water not have cock muscle completely, soaks two days later, and taking-up water is rinsed well；Again put back to leaching container, add dehydrated alcohol and the mixed solution of dimethylbenzene that volume ratio is 1:1, dehydrated alcohol and dimethylbenzene mixed solution is made not to have cock muscle completely, changed every 24 hours and once soak the dehydrated alcohol and dimethylbenzene mixed solution that volume ratio used is 1:1, soak and take out two days later；During degreasing operation, obturage leaching container with Polypropylence Sheet, prevent solution evaporation, in order to avoid operator are damaged；
Process after step 6, defat: the cock muscle after defat is placed on dark ventilation and dries in the air 7 days, then be that 5% detergent solution soaks 2h by the cock muscle mass percent dried well, then use clean water three times；
Step 7, above-mentioned steps seven is processed after cock muscle insert the hydrogen peroxide after dilution, take out after soaking 5 days, rinse well with clear water, dry；
Step 8, with glue by fixing for muscle on a glass, posture when making muscle keep cock to stand, be then placed in preparation jar, i.e. prepare the whole-body muscle specimen of mature rooster.
Embodiment
2
A kind of whole-body muscle Slide processing of mature rooster, its concrete preparation process is:
Step one selects the corpse of mature rooster；
Step 2, at the corpse abdominal cavity skin of cock, open the mouth of a diameter of 1.2 cm with scalpel, expose heart；On right auricle, the osculum of a diameter of 0.3cm is cut with operating scissors, perfusion syringe needle is inserted in the left ventricle apex of the heart, fix with mosquito forceps, quick filling 1000 ml normal saline, after blood is rinsed well, the most successively pouring quality percentage ratio be 20%, 40%, 60%, 80% and each 800ml of Aqueous Solutions of Polyethylene Glycol of 90%, first quick and back slow, the most about 2 hours；
Step 3, peeling: faced upward by cock corpse and be put on dissecting table, cut the osculum of a diameter of 0.5cm inside tarsal joint with operating scissors, then cuts off cock skin inside cut osculum to anus front；It is then peeled off cock skin, by the method for drill drift, skin is sloughed to cervical region so that it is become dermatotome housing type outwardly；Cut off the auricular cartilage of ear again, separate eye and rostral surrounding skin, peel off skin with caution at the apex of the wing, skin is completely stripped, finally gill；
Step 4, separation cock whole-body muscle: use scalpel to isolate the whole-body muscle of cock；Concrete operation method is: peels off and isolates scalp flesh, musculus cutaneus colli and trunk musculus cutaneus under head, cervical region and body skin after superficial fascia.Wipe out fascia and connective tissue along muscle Germ distribution, isolate the facial muscle of head, masticatory muscles and pharyngeal flesh；At cervical region separation cervical muscle, musculus hyoideus, multiple flesh and digastric.At trunk, after boundary, terminal stripping fascia, connective tissue, blood vessel and the muscle of muscle, separate the muscle such as flesh on Intercostal muscle, levatores costarum, scalenus, longus colli, omoplate (flesh after shoulder arm), latissimus dorsi m., Intercostal muscle, ilium tibialis anterior (sartorius m.), gluteus medius, tail elevator, musculus depressor coccygis, ilium shin musculus lateralis externi and ilium calf flesh (biceps femoris), musculus pronator profundus, Ulnar Wrist musculus flexor, arm triceps muscle, arm biceps, musculus pronator sublimis, palm oar side extensor, ala longue, palm oar side extensor, musculus extensor digitorum, palm side extensor and outside upper ankle cubitalis.In drumsticks portion, separate musculus flexor and gracilis etc. inside ilium shin musculus lateralis externi, ilium calf flesh, thigh lateral musculus flexor, ilium tibialis anterior, pectineus, adductor, toe；
Step 5, defat: by isolated cock muscle, rinse well with water, be placed in leaching container, be subsequently adding the ethanol water that mass percent is 80%, makes ethanol water not have cock muscle completely, soaks two days later, and taking-up water is rinsed well；Again put back to leaching container, add dehydrated alcohol and the mixed solution of dimethylbenzene that volume ratio is 1:1, dehydrated alcohol and dimethylbenzene mixed solution is made not to have cock muscle completely, changed every 12 hours and once soak the dehydrated alcohol and dimethylbenzene mixed solution that volume ratio used is 1:1, soak and take out two days later；During degreasing operation, obturage leaching container with Polypropylence Sheet, prevent solution evaporation, in order to avoid operator are damaged；
Process after step 6, defat: the cock muscle after defat is placed on dark ventilation and dries in the air 5 days, then be that 3% detergent solution soaks 1h by the cock muscle mass percent dried well, then use clean water three times；
Step 7, above-mentioned steps seven is processed after cock muscle insert the hydrogen peroxide after dilution, take out after soaking 2 days, rinse well with clear water, dry；
Step 8, with glue by fixing for muscle on a glass, posture when making muscle keep cock to stand, be then placed in preparation jar, i.e. prepare the whole body whole-body muscle specimen of mature rooster.
Embodiment
3
A kind of whole-body muscle Slide processing of mature rooster, its concrete preparation process is:
Step one, the corpse of selection mature rooster；
Step 2, at the corpse abdominal cavity skin of cock, open the mouth of a diameter of 1.0cm with scalpel, expose heart；On right auricle, the osculum of a diameter of 0.35cm is cut with operating scissors, perfusion syringe needle is inserted in the left ventricle apex of the heart, fix with mosquito forceps, quick filling 1000 ml normal saline, after blood is rinsed well, the most successively pouring quality percentage ratio be 20%, 40%, 60%, 80% and each 800ml of Aqueous Solutions of Polyethylene Glycol of 90%, first quick and back slow, the most about 2 hours；
Step 3, peeling: faced upward by cock corpse and be put on dissecting table, cut the osculum of a diameter of 0.8cm inside tarsal joint with operating scissors, then cuts off cock skin inside cut osculum to anus front；It is then peeled off cock skin, by the method for drill drift, skin is sloughed to cervical region so that it is become dermatotome housing type outwardly；Cut off the auricular cartilage of ear again, separate eye and rostral surrounding skin, peel off skin with caution at the apex of the wing, skin is completely stripped, finally gill；
Step 4, separation cock whole-body muscle: use scalpel to isolate the whole-body muscle of cock；Concrete operation method is: peels off and isolates scalp flesh, musculus cutaneus colli and trunk musculus cutaneus under head, cervical region and body skin after superficial fascia.Wipe out fascia and connective tissue along muscle Germ distribution, isolate the facial muscle of head, masticatory muscles and pharyngeal flesh；At cervical region separation cervical muscle, musculus hyoideus, multiple flesh and digastric.At trunk, after boundary, terminal stripping fascia, connective tissue, blood vessel and the muscle of muscle, separate the muscle such as flesh on Intercostal muscle, levatores costarum, scalenus, longus colli, omoplate (flesh after shoulder arm), latissimus dorsi m., Intercostal muscle, ilium tibialis anterior (sartorius m.), gluteus medius, tail elevator, musculus depressor coccygis, ilium shin musculus lateralis externi and ilium calf flesh (biceps femoris), musculus pronator profundus, Ulnar Wrist musculus flexor, arm triceps muscle, arm biceps, musculus pronator sublimis, palm oar side extensor, ala longue, palm oar side extensor, musculus extensor digitorum, palm side extensor and outside upper ankle cubitalis.In drumsticks portion, separate musculus flexor and gracilis etc. inside ilium shin musculus lateralis externi, ilium calf flesh, thigh lateral musculus flexor, ilium tibialis anterior, pectineus, adductor, toe；
Step 5, defat: by isolated cock muscle, rinse well with water, be placed in leaching container, be subsequently adding the ethanol water that mass percent is 80%, makes ethanol water not have cock muscle completely, soaks two days later, and taking-up water is rinsed well；Again put back to leaching container, add dehydrated alcohol and the mixed solution of dimethylbenzene that volume ratio is 1:1, dehydrated alcohol and dimethylbenzene mixed solution is made not to have cock muscle completely, changed every 18 hours and once soak the dehydrated alcohol and dimethylbenzene mixed solution that volume ratio used is 1:1, soak and take out two days later；During degreasing operation, obturage leaching container with Polypropylence Sheet, prevent solution evaporation, in order to avoid operator are damaged；
Process after step 6, defat: the cock muscle after defat is placed on dark ventilation and dries in the air 6 days, then be that 4% detergent solution soaks 1.5h by the cock muscle mass percent dried well, then use clean water three times；
Step 7, above-mentioned steps seven is processed after cock muscle insert the hydrogen peroxide after dilution, take out after soaking 3 days, rinse well with clear water, dry；
Step 8, with glue by fixing for muscle on a glass, posture when making muscle keep cock to stand, be then placed in preparation jar, i.e. prepare the whole body whole-body muscle specimen of mature rooster.
Embodiment
4
A kind of whole-body muscle Slide processing of mature rooster, its concrete preparation process is:
Step one, the corpse of selection mature rooster；
Step 2, at the corpse abdominal cavity skin of cock, open the mouth of a diameter of 1.0cm with scalpel, expose heart；On right auricle, the osculum of a diameter of 0.35cm is cut with operating scissors, perfusion syringe needle is inserted in the left ventricle apex of the heart, fix with mosquito forceps, quick filling 1000 ml normal saline, after blood is rinsed well, the most successively pouring quality percentage ratio be 20%, 40%, 60%, 80% and each 800ml of Aqueous Solutions of Polyethylene Glycol of 90%, first quick and back slow, the most about 2 hours；
Step 3, peeling: faced upward by cock corpse and be put on dissecting table, cut the osculum of a diameter of 0.8cm inside tarsal joint with operating scissors, then cuts off cock skin inside cut osculum to anus front；It is then peeled off cock skin, by the method for drill drift, skin is sloughed to cervical region so that it is become dermatotome housing type outwardly；Cut off the auricular cartilage of ear again, separate eye and rostral surrounding skin, peel off skin with caution at the apex of the wing, skin is completely stripped, finally gill；
Step 4, separation cock whole-body muscle: use scalpel to isolate the whole-body muscle of cock；Concrete operation method is: peels off and isolates scalp flesh, musculus cutaneus colli and trunk musculus cutaneus under head, cervical region and body skin after superficial fascia.Wipe out fascia and connective tissue along muscle Germ distribution, isolate the facial muscle of head, masticatory muscles and pharyngeal flesh；At cervical region separation cervical muscle, musculus hyoideus, multiple flesh and digastric.At trunk, after boundary, terminal stripping fascia, connective tissue, blood vessel and the muscle of muscle, separate the muscle such as flesh on Intercostal muscle, levatores costarum, scalenus, longus colli, omoplate (flesh after shoulder arm), latissimus dorsi m., Intercostal muscle, ilium tibialis anterior (sartorius m.), gluteus medius, tail elevator, musculus depressor coccygis, ilium shin musculus lateralis externi and ilium calf flesh (biceps femoris), musculus pronator profundus, Ulnar Wrist musculus flexor, arm triceps muscle, arm biceps, musculus pronator sublimis, palm oar side extensor, ala longue, palm oar side extensor, musculus extensor digitorum, palm side extensor and outside upper ankle cubitalis.In drumsticks portion, separate musculus flexor and gracilis etc. inside ilium shin musculus lateralis externi, ilium calf flesh, thigh lateral musculus flexor, ilium tibialis anterior, pectineus, adductor, toe；
Step 5, defat: isolated cock muscle is placed in leaching container, pours gasoline into, airtight, soak and carry out defat in about 7 days.During degreasing operation, obturage leaching container with Polypropylence Sheet, prevent solution evaporation, in order to avoid operator are damaged；
Process after step 6, defat: the cock muscle after defat is placed on dark ventilation and dries in the air 6 days, then be that 4% detergent solution soaks 1.5h by the cock muscle mass percent dried well, then use clean water three times；
Step 7, above-mentioned steps seven is processed after cock muscle insert the hydrogen peroxide after dilution, take out after soaking 3 days, rinse well with clear water, dry；
Step 8, with glue by fixing for muscle on a glass, posture when making muscle keep cock to stand, be then placed in preparation jar, i.e. prepare the whole-body muscle specimen of mature rooster.
Claims (2)
1. the whole-body muscle Slide processing of a mature rooster, it is characterised in that: its concrete preparation process is:
Step one, solution are prepared: hydrogen peroxide is diluted to distilled water the solution that mass percent is 3.5% of hydrogen peroxide, prepares the hydrogen peroxide after dilution, standby；Weigh detergent, add distilled water, prepare the detergent solution that mass percent is 3%～5%；Weigh the Macrogol 600 of Different Weight respectively, add distilled water and prepare the Aqueous Solutions of Polyethylene Glycol that mass percent is 20%, 40%, 60%, 80% and 90% respectively；Prepared solution is wanted matching while using or is placed in 4 DEG C of preservations；
Step 2, the corpse of selection mature rooster；
Step 3, at the corpse abdominal cavity skin of cock, open the mouth of a diameter of 0.8 ~ 1.2cm with scalpel, expose heart；On right auricle, the osculum of a diameter of 0.3 ~ 0.4 cm is cut with operating scissors, perfusion syringe needle is inserted in the left ventricle apex of the heart, fix with mosquito forceps, irrigate 1000 ml normal saline, after blood is rinsed well, pouring quality percentage ratio is each 800ml of Aqueous Solutions of Polyethylene Glycol of 20%, 40%, 60%, 80% and 90% the most successively, continues 2 hours；
Step 4, peeling: faced upward by cock corpse and be put on dissecting table, cut the osculum of a diameter of 0.5 ~ 1cm inside tarsal joint with operating scissors, then cuts off cock skin inside cut osculum to anus front；It is then peeled off cock skin, by the method for drill drift, skin is sloughed to cervical region so that it is become dermatotome housing type outwardly；Cut off the auricular cartilage of ear again, separate eye and rostral surrounding skin, peel off skin with caution at the apex of the wing, skin is completely stripped, finally gill；
Step 5, separation cock whole-body muscle: use scalpel to isolate the whole-body muscle of cock；
Step 6, defat: by isolated cock muscle, rinse well with water, be placed in leaching container, be subsequently adding the ethanol water that mass percent is 80%, makes ethanol water not have cock muscle completely, soaks two days later, and taking-up water is rinsed well；Again put back to leaching container, add dehydrated alcohol and the mixed solution of dimethylbenzene that volume ratio is 1:1, dehydrated alcohol and dimethylbenzene mixed solution is made not to have cock muscle completely, changed every 12 ~ 24 hours and once soak the dehydrated alcohol and dimethylbenzene mixed solution that volume ratio used is 1:1, soak and take out two days later；
In the operating process of described defat, obturage leaching container with Polypropylence Sheet, prevent solution evaporation；
Process after step 7, defat: the cock muscle after defat is placed on dark ventilation and dries in the air 5～7 days, then be that 3%～5% detergent solution soaks 1～2h by the cock muscle mass percent dried well, then use clean water three times；
Step 8, above-mentioned steps seven is processed after cock muscle insert the hydrogen peroxide after dilution, take out after soaking 2～5 days, rinse well with clear water, dry；
Step 9, with glue by fixing for muscle on a glass, posture when making muscle keep cock to stand, be then placed in preparation jar, i.e. prepare the whole-body muscle specimen of mature rooster.
The whole-body muscle Slide processing of a kind of mature rooster the most as claimed in claim 1, it is characterized in that: the described degreasing method in step 6 uses with substitute mode: isolated cock muscle specimen is placed in leaching container, pour gasoline into, airtight, soak and carry out defat in 7 days.
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| CN103824494B (en) * | 2014-01-29 | 2015-10-28 | 河南科技大学 | A kind of preparation method of mature rooster sample |
| CN105037745B (en) * | 2015-07-14 | 2017-06-16 | 河南科技大学 | A kind of polyethylene glycol lignin solvent and its application in lignin separation extraction |
| CN114203002A (en) * | 2021-12-29 | 2022-03-18 | 北京麋鹿生态实验中心 | Manufacturing method of endangered wild animal plasticized specimen |
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