CN103380768B - Manufacturing method of adult ram nervous system specimen - Google Patents
Manufacturing method of adult ram nervous system specimen Download PDFInfo
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- CN103380768B CN103380768B CN201310280718.6A CN201310280718A CN103380768B CN 103380768 B CN103380768 B CN 103380768B CN 201310280718 A CN201310280718 A CN 201310280718A CN 103380768 B CN103380768 B CN 103380768B
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Abstract
The invention discloses a manufacturing method of an adult ram nervous system specimen. The manufacturing method comprises following steps: selecting a carcass of a ram which is executed by fixed perfusion after anaesthesia; peeling off the skin of the ram carcass, and completely separating the nervous system from the ram carcass; subjecting the ram nervous system to derosination by a mixed solution of absolute ethyl alcohol and xylene with a ratio of 1:1; airing for 5 to 7 days at a dark and ventilated place; immersing the aired ram nervous system into a washing powder solution with a concentration of 3 to 5% for 1 to 2h, washing the ram nervous system with clean water for three times, and bleaching with diluted hydrogen peroxide and washing; fixing the nervous system on a glass plate with glue, wherein the posture of the nervous system is identical with the posture of the nervous system when the ram is in a standing posture; and then placing the nervous system into a specimen jar so as to obtain the adult ram nervous system specimen. The manufacturing method is simple; cost is low; the obtained adult ram nervous system specimen is easy for preservation; the structure of the adult ram nervous system specimen is complete and beautiful; and the posture and the position are clear and natural.
Description
Technical field
The present invention relates to a kind of Slide processing, specifically the nervous system Slide processing of a kind of adult ram.
Background technology
Zoological specimens, be keep former state in kind or through arrangement can for referential when study, research can individuality representatively in one species, also can be used as the important references of universal publicity of scientific research, teaching and Sci-tech Knowledge.Scientific value, ornamental value and reserve value that what animal and tissue specimen contained can not be substituted, other arts work of erect image equally enter into family, become the indoor new ornament of people.One makes exquisite sample and not only brings people's knowledge, also brings the enjoyment of people's art simultaneously.Perfect animal nervous system sample is a vivid practical teaching aid, can be again a consummate art work, it represent colleges and universities, a subject development level and grasp the professional skill of this disciplinary study person.Preparation of specimen is also a research work, in manufacturing process, can constantly discover problems and solve them, and creates new technology, can broaden one's knowledge simultaneously, tempers thinking and the manipulative ability of participant.Be made into whole nervous system sample, can demonstrate whole body nervous system structures, sample structure is attractive in appearance, and form knows nature, and shows the profile of the form of nervous centralis brain, structure that ditch returns, spinal cord well.Sample not only in " Zootomy " teaching as directly perceived, vivid teaching aid, and can allow students' participation in manufacturing process, allow student understand and grasp preparation of specimen's process, simultaneously the starting of training student, beat one's brains ability.Contribute to the understanding to nervous system overview, maincenter and perineural contour structures are grasped, good help is all played to the understanding of the Name & Location relation of neural nerve fibre bundle, contribute to improving teaching efficiency.
Summary of the invention
The present invention seeks to as solving the problems of the technologies described above, providing the nervous system Slide processing of a kind of adult ram, its preparation method is simple, and prepared sample can demonstrate whole body nervous system structures, and structure is attractive in appearance, and form knows nature.
A nervous system Slide processing for adult ram, its concrete preparation process is:
Step one, solution preparation: the mass percent with distilled water hydrogen peroxide being diluted to hydrogen peroxide is the solution of 3%, prepares the hydrogen peroxide after dilution, for subsequent use; Take washing powder, add distilled water, prepare the detergent solution that mass percent is 3% ~ 5%; Prepared solution is wanted matching while using or is placed in 4 DEG C of preservations;
After step 2, selection anesthesia, through the corpse of the adult ram that perfusion fixation is put to death;
Step 3, peeling: faced upward by ram corpse and be put on autopsy table, cut the osculum that diameter is 0.5 ~ 1cm respectively with operating scissors inside front wrist joint and hind leg tarsal joint, inside cut osculum, then cut off ram skin Zhiyin side in front of the door respectively; Then be separated ram skin, by the method for drill drift, skin sloughed to neck, become dermatotome housing type outwardly; Cut off the auricular cartilage of ear again, be separated eye and mouth skin of lip, by complete for skin stripping, gill after finally isolating nervus visceralis;
Step 4, the neural nerve of separation ram: adopt bone saw to cut ram head, divest skull, retain brain dura mater, arachnoid and Pial matter, isolate brain, cerebellum, brain stem; Reject muscle, divest vertebra, retain ridge dura mater, arachnoid and ridge mantle, and remove connective tissue and blood vessel, isolate spinal cord; When peeling off neural along the trend of nerve and distribution, cut off muscle, remove connective tissue along the interstitial between muscle, peel off blood vessel, be separated the serous coat being coated on neural outside, expose ram nerve and by its complete separation;
Step 5, degreasing: by isolated ram nervous system nerves, rinse well with water, be placed in infuse container, then the ethanol water that mass percent is 80% is added, ethanol water is made not have ram nervous system nerves completely, soak two days later, taking-up water is rinsed well, picks clean residual muscle with scalpel; Again put back to infuse container, adding volume ratio is again the absolute ethyl alcohol of 1:1 and the mixed solution of dimethylbenzene, absolute ethyl alcohol and dimethylbenzene mixed solution is made not to have ram nervous system nerves completely, changed every 12 ~ 24 hours and once soak the absolute ethyl alcohol and dimethylbenzene mixed solution that volume ratio used is 1:1, soak and take out two days later; Or isolated ram nervous system nerves is placed in infuse container, pours gasoline into, airtight, soak and carry out degreasing in about 7 days.In degreasing operation process, to obturage infuse container with Polypropylence Sheet, prevent solution evaporation, in order to avoid damage operating personnel;
Process after step 6, degreasing: the ram nerve after degreasing is placed on dark ventilation and dries in the air 5 ~ 7 days, then by the ram nerve mass percent dried well be 3% ~ 5% detergent solution soak 1 ~ 2h, then use clean water three times;
Step 7, above-mentioned steps six is processed after ram nerve insert the hydrogen peroxide after dilution, soaks 2 ~ 5 days, when ram nerve bleaches taking-up, rinse well with clear water, dry;
Step 8, with glue by neurofixation on a glass, make neural posture when keeping ram to stand, then put in specimen jar, namely prepare the nervous system sample of adult ram.
Beneficial effect is:
1, the present invention grows up the nervous system Slide processing of ram, and preparation method is simple, easy, with low cost; Adopt ethanol water, absolute ethyl alcohol and dimethylbenzene or gasoline to carry out ungrease treatment to ram nervous system nerves, degreasing is thorough, can prevent from using permanent and preserve, on ram nervous system nerves, grease slowly oozes out, pollute mouldy, make sample impaired, hinder use and attractive in appearance.Adopt the mass percent of hydrogen oxide to be that after the dilution of 3%, hydrogen peroxide is bleached, effect is better and to ram nervous system nerves not damaged.
2, the present invention grows up the nervous system sample of the nervous system Slide processing of ram, prepared ram, is easy to preserve, and structural integrity is attractive in appearance, and morphologic localization knows nature.The form of nervous centralis brain and ditch, the morphosis returned, spinal cord, all clearly illustrated, each nerve cord perineural represents all well and is fixed on the physical slot of sheep body.The position relationship of brain, cerebral nerve, spinal cord, spinal nerve and nervus visceralis all knows nature.Solve the problem being difficult to the overall picture seeing cerebral nerve and spinal nerve.Nervous system is the Focal point and difficult point part in human anatomy teaching, and the nervous system in Systematic Anatomy teaching is mainly theoretical sees that sample is few more, and topologic Students in Teaching does not generally dissect brain and spinal cord.This sample preferably resolves this difficult problem.Both shortened the teachers' instruction time, and excited again the learning interest of student, improve teaching efficiency.
Embodiment
A nervous system Slide processing for adult ram, its concrete preparation process is:
Step one, solution preparation: the mass percent with distilled water hydrogen peroxide being diluted to hydrogen peroxide is the solution of 3%, prepares the hydrogen peroxide after dilution, for subsequent use; Take washing powder, add distilled water, prepare the detergent solution that mass percent is 3% ~ 5%; Prepared solution is wanted matching while using or is placed in 4 DEG C of preservations;
After step 2, selection anesthesia, through the corpse of the adult ram that perfusion fixation is put to death;
Step 3, peeling: faced upward by ram corpse and be put on autopsy table, cut the osculum that diameter is 0.5 ~ 1cm respectively with operating scissors inside front wrist joint and hind leg tarsal joint, inside cut osculum, then cut off ram skin Zhiyin side in front of the door respectively; Then be separated ram skin, by the method for drill drift, skin sloughed to neck, become dermatotome housing type outwardly; Cut off the auricular cartilage of ear again, be separated eye and mouth skin of lip, by complete for skin stripping, gill after finally isolating nervus visceralis;
Step 4, the neural nerve of separation ram: adopt bone saw to cut ram head, divest skull, retain brain dura mater, arachnoid and Pial matter, isolate brain, cerebellum, brain stem; Reject muscle, divest vertebra, retain ridge dura mater, arachnoid and ridge mantle, and remove connective tissue and blood vessel, isolate spinal cord; When peeling off neural along the trend of nerve and distribution, cut off muscle, remove connective tissue along the interstitial between muscle, peel off blood vessel, be separated the serous coat being coated on neural outside, expose ram nerve and by its complete separation;
Step 5, degreasing: by isolated ram nervous system nerves, rinse well with water, be placed in infuse container, then the ethanol water that mass percent is 80% is added, ethanol water is made not have ram nervous system nerves completely, soak two days later, taking-up water is rinsed well, picks clean residual muscle with scalpel; Again put back to infuse container, adding volume ratio is again the absolute ethyl alcohol of 1:1 and the mixed solution of dimethylbenzene, absolute ethyl alcohol and dimethylbenzene mixed solution is made not to have ram nervous system nerves completely, changed every 12 ~ 24 hours and once soak the absolute ethyl alcohol and dimethylbenzene mixed solution that volume ratio used is 1:1, soak and take out two days later; Or isolated ram nervous system nerves is placed in infuse container, pours gasoline into, airtight, soak and carry out degreasing in about 7 days.In degreasing operation process, to obturage infuse container with Polypropylence Sheet, prevent solution evaporation, in order to avoid damage operating personnel;
Process after step 6, degreasing: the ram nerve after degreasing is placed on dark ventilation and dries in the air 5 ~ 7 days, then by the ram nerve mass percent dried well be 3% ~ 5% detergent solution soak 1 ~ 2h, then use clean water three times;
Step 7, above-mentioned steps six is processed after ram nerve insert the hydrogen peroxide after dilution, soaks 2 ~ 5 days, when ram nerve bleaches taking-up, rinse well with clear water, dry;
Step 8, with glue by neurofixation on a glass, make neural posture when keeping ram to stand, then put in specimen jar, namely prepare the nervous system sample of adult ram.
Isolated ram nerve comprises central nervous system nerve and brain and spinal cord, and other are neural.Wherein, brain is positioned at cranial cavity and is connected with eyes by optic nerve forward, and spinal cord is positioned at intraspinal tube; It is totally 31 right that spinal cord sends spinal nerve: neck 8 to, chest 12 to, waist 5 to, recommend 5 right to, tail 1.Front, rear is all borrowed to be connected with spinal cord to spinal nerve.Front and rear is synthesized a spinal nerve at intervertebral foramen place.Namely spinal nerve is divided into two, front and back after going out foramina intervertebrale, after intervertebral foramen glass muscle, manadesma, isolate nerve.The sections that 2nd ~ 12 pairs of rami anteriores nervorum thoracaliums press rib and thoracic vertebrae distributes, and is called intercostal nerve (wherein the 12nd rami anteriores nervorum thoracalium claims subcostal nerve).Front of all the other spinal nerves connects formation four neuropiles each other: cervical plexus, brachial plexus (Main Branches has center, oar, ulnar nerve, is distributed to upper limbs), lumbal plexus, recommend clump.Sciatic nerve is the maximum branch that lumbosacral plexus is distributed to lower limb and goes.Olfactory nerve, optic nerve, oculomotor nerve, trochlear nerve, trigeminal neuralgia, abducent nerve, facial nerve, nervus statoacusticus, glossopharyngeal nerve, vagus nerve, accessory nerve and hypoglossal nerve, wherein trigeminal neuralgia is made up of ophthalmic nerve, maxillary nerve and mandibular nerve respectively.12 pairs of cerebral nerve are connected to the different parts of brain, and by basis cranii porous dehiscence come in and go out cranial cavity.Specifically: olfactory nerve enters cranial cavity through sieve plate; Optic nerve enters cranial cavity through canalis opticus; Oculomotor nerve, trochlear nerve, ophthalmic nerve, abducent nerve enter cranial cavity through superior orbital fissure; Maxillary nerve is through circular hole, and mandibular nerve enters cranial cavity through oval foramen; Facial nerve, nervus statoacusticus enter cranial cavity through porus acusticus internus; Glossopharyngeal nerve, vagus nerve, accessory nerve enter cranial cavity through jugular foramen; Hypoglossal nerve enters cranial cavity through internal opening of hypoglossal canal.
Following examples can make the present invention of those skilled in the art's complete understanding, but do not limit the present invention in any way.
embodiment 1
A nervous system Slide processing for adult ram, its concrete preparation process is:
Step one, the adult ram one selecting build plentiful and substantial, healthy and strong, claim its body weight;
Step 2, solution preparation: the body weight according to ram takes yellow Jackets, standard is that every 1kg goat takes yellow Jackets 45mg, is dissolved in stroke-physiological saline solution and is made into the Nembutal sodium solution that mass fraction is 3%, for subsequent use; The mass percent with distilled water formalin solution being diluted to formaldehyde is the solution of 7%, prepares the formalin solution after dilution, for subsequent use; The mass percent with distilled water hydrogen peroxide being diluted to hydrogen peroxide is the solution of 3%, prepares the hydrogen peroxide after dilution, for subsequent use; Take washing powder, add distilled water, prepare the detergent solution that mass percent is 3% ~ 5%; Prepared solution want matching while using or be placed in 4 DEG C for subsequent use;
Step 3, perfusion fixation: draw the Nembutal sodium solution prepared by above-mentioned steps two with syringe, adopt intraperitoneal injection anesthesia ram; Then open at the skin place of ram jugular vein ditch the mouth that diameter is 3 cm with scalpel, expose arteria carotis communis; On arteria carotis communis, the osculum that diameter is 0.3cm is cut with operating scissors, perfusion syringe needle is inserted in osculum, fix with haemostatic clamp, quick filling 3000ml physiological saline, treat that blood is rinsed well, after namely liver bleaches, pour into the formalin solution after 5000 ml dilutions, first quick and back slow, about two hours are continued;
Step 4, peeling: ram corpse is faced upward and is put on autopsy table, being faced upward by ram corpse is immediately put on autopsy table, inside front wrist joint and hind leg tarsal joint, cutting the osculum that diameter is 0.5cm respectively with operating scissors, then inside osculum, cutting off ram skin Zhiyin side in front of the door respectively from cutting; Then be separated ram skin, then a people grabs two hind legs, and skin is sloughed to neck by the method for another people's drill drift, becomes dermatotome housing type outwardly; Cut off the auricular cartilage of ear again, be separated eye and mouth skin of lip, by complete for skin stripping, gill after finally isolating nervus visceralis;
Step 5, the neural nerve of separation ram: adopt bone saw to cut ram head, divest skull, retain brain dura mater, arachnoid and Pial matter, isolate brain, cerebellum; Reject chest, waist back of the body waist eye muscle, iliocostalis, head longissimus dorsi muscle, greater psoas muscle and smaller psoas muscle etc., divest vertebra, retain ridge dura mater, arachnoid and ridge mantle, and remove connective tissue and blood vessel, isolate spinal cord.When trend along nerve peels off nerve with distribution, carefully cut off muscle, remove connective tissue along the interstitial between muscle, peel off blood vessel, be separated the serous coat be coated on outside nerve, abundant NE complete separation is neural.
Step 6, by isolated ram nervous system nerves, rinse well with water, be placed in infuse container, then the ethanol water that mass percent is 80% is added, ethanol water is made not have ram nervous system nerves completely, soak two days later, taking-up water is rinsed well, picks clean residual muscle with scalpel; Again put back to infuse container, add absolute ethyl alcohol and dimethylbenzene mixed solution that volume ratio is 1:1 again, absolute ethyl alcohol and dimethylbenzene mixed solution is made not to have ram nervous system nerves completely, changed every 12 hours and once soak the absolute ethyl alcohol and dimethylbenzene mixed solution that volume ratio used is 1:1, soak and take out two days later;
Process after step 7, degreasing: the ram nerve after degreasing is placed on dark ventilation and dries in the air 5 days, makes neural residual dimethylbenzene volatilization in order to avoid damage operating personnel; Be the detergent solution immersion 1 of 3% again by the ram nerve mass percent dried well, then use clean water three times, thoroughly except removal xylene;
Step 8, by cleaning after ram nerve insert the hydrogen peroxide after dilution, soaks 2 days, when ram nerve bleaches taking-up, rinse well with clear water, dry.
Step 9, with glue by neurofixation on a glass, make neural posture when keeping ram to stand, so that the displaying that sample is lively and active; Then put in specimen jar, namely prepare the present invention and to grow up the nervous system sample of ram;
embodiment 2
A nervous system Slide processing for adult ram, its concrete preparation process is:
Step one, the adult ram one selecting build plentiful and substantial, healthy and strong, claim its body weight;
Step 2, solution preparation: the body weight according to ram takes yellow Jackets, standard is that every 1kg goat takes yellow Jackets 40mg, is dissolved in stroke-physiological saline solution and is made into the Nembutal sodium solution that mass fraction is 2%, for subsequent use; The mass percent with distilled water formalin solution being diluted to formaldehyde is the solution of 7%, prepares the formalin solution after dilution, for subsequent use; The mass percent with distilled water hydrogen peroxide being diluted to hydrogen peroxide is the solution of 3%, prepares the hydrogen peroxide after dilution, for subsequent use; Take washing powder, add distilled water, prepare the detergent solution that mass percent is 3% ~ 5%; Prepared solution want matching while using or be placed in 4 DEG C for subsequent use;
Step 3, perfusion fixation: draw the Nembutal sodium solution prepared by above-mentioned steps two with syringe, adopt intraperitoneal injection anesthesia ram; Then open at the skin place of ram jugular vein ditch the mouth that diameter is 4cm with scalpel, expose arteria carotis communis; On arteria carotis communis, the osculum that diameter is 0.4cm is cut with operating scissors, perfusion syringe needle is inserted in osculum, fix with haemostatic clamp, quick filling 3500ml physiological saline, treat that blood is rinsed well, after namely liver bleaches, pour into the formalin solution after 5000 ml dilutions, first quick and back slow, about two hours are continued;
Step 4, peeling: ram corpse is faced upward and is put on autopsy table, being faced upward by ram corpse is immediately put on autopsy table, inside front wrist joint and hind leg tarsal joint, cutting the osculum that diameter is 0.8cm respectively with operating scissors, then inside osculum, cutting off ram skin Zhiyin side in front of the door respectively from cutting; Then be separated ram skin, then a people grabs two hind legs, and skin is sloughed to neck by the method for another people's drill drift, becomes dermatotome housing type outwardly; Cut off the auricular cartilage of ear again, be separated eye and mouth skin of lip, by complete for skin stripping, gill after finally isolating nervus visceralis;
Step 5, the neural nerve of separation ram: adopt bone saw to cut ram head, divest skull, retain brain dura mater, arachnoid and Pial matter, isolate brain, cerebellum; Reject chest, waist back of the body waist eye muscle, iliocostalis, head longissimus dorsi muscle, greater psoas muscle and smaller psoas muscle etc., divest vertebra, retain ridge dura mater, arachnoid and ridge mantle, and remove connective tissue and blood vessel, isolate spinal cord.When trend along nerve peels off nerve with distribution, carefully cut off muscle, remove connective tissue along the interstitial between muscle, peel off blood vessel, be separated the serous coat be coated on outside nerve, abundant NE complete separation is neural;
Step 6, by isolated ram nervous system nerves, rinse well with water, be placed in infuse container, then the ethanol water that mass percent is 80% is added, ethanol water is made not have ram nervous system nerves completely, soak two days later, taking-up water is rinsed well, picks clean residual muscle with scalpel; Again put back to infuse container, add absolute ethyl alcohol and dimethylbenzene mixed solution that volume ratio is 1:1 again, absolute ethyl alcohol and dimethylbenzene mixed solution is made not to have ram nervous system nerves completely, changed every 20 hours and once soak the absolute ethyl alcohol and dimethylbenzene mixed solution that volume ratio used is 1:1, soak and take out two days later;
Process after step 7, degreasing: the ram nerve after degreasing is placed on dark ventilation and dries in the air 6 days, makes neural residual dimethylbenzene volatilization in order to avoid damage operating personnel; Be the detergent solution immersion 1.5h of 4% again by the ram nerve mass percent dried well, then use clean water three times, thoroughly except removal xylene;
Step 8, by cleaning after ram nerve insert the hydrogen peroxide after dilution, soaks 4 days, when ram nerve bleaches taking-up, rinse well with clear water, dry;
Step 9, with glue by neurofixation on a glass, make neural posture when keeping ram to stand, so that the displaying that sample is lively and active; Then put in specimen jar, namely prepare the present invention and to grow up the nervous system sample of ram.
embodiment 3
A nervous system Slide processing for adult ram, its concrete preparation process is:
Step one, the adult ram one selecting build plentiful and substantial, healthy and strong, claim its body weight;
Step 2, solution preparation: the body weight according to ram takes yellow Jackets, standard is that every 1kg goat takes yellow Jackets 30mg, is dissolved in stroke-physiological saline solution and is made into the Nembutal sodium solution that mass fraction is 1%, for subsequent use; The mass percent with distilled water formalin solution being diluted to formaldehyde is the solution of 7%, prepares the formalin solution after dilution, for subsequent use; The mass percent with distilled water hydrogen peroxide being diluted to hydrogen peroxide is the solution of 3%, prepares the hydrogen peroxide after dilution, for subsequent use; Take washing powder, add distilled water, prepare the detergent solution that mass percent is 3% ~ 5%; Prepared solution want matching while using or be placed in 4 DEG C for subsequent use;
Step 3, perfusion fixation: draw the Nembutal sodium solution prepared by above-mentioned steps two with syringe, adopt intraperitoneal injection anesthesia ram then to open at the skin place of ram jugular vein ditch the mouth that diameter is 5 cm with scalpel, expose arteria carotis communis; On arteria carotis communis, the osculum that diameter is 0.5cm is cut with operating scissors, perfusion syringe needle is inserted in osculum, fix with haemostatic clamp, quick filling 4000ml physiological saline, treat that blood is rinsed well, after namely liver bleaches, pour into the formalin solution after 5000 ml dilutions, first quick and back slow, about two hours are continued;
Step 4, peeling: ram corpse is faced upward and is put on autopsy table, being faced upward by ram corpse is immediately put on autopsy table, inside front wrist joint and hind leg tarsal joint, cutting the osculum that diameter is 1cm respectively with operating scissors, then inside osculum, cutting off ram skin Zhiyin side in front of the door respectively from cutting; Then be separated ram skin, then a people grabs two hind legs, and skin is sloughed to neck by the method for another people's drill drift, becomes dermatotome housing type outwardly; Cut off the auricular cartilage of ear again, be separated eye and mouth skin of lip, by complete for skin stripping, gill after finally isolating nervus visceralis;
Step 5, the neural nerve of separation ram: adopt bone saw to cut ram head, divest skull, retain brain dura mater, arachnoid and Pial matter, isolate brain, cerebellum; Reject chest, waist back of the body waist eye muscle, iliocostalis, head longissimus dorsi muscle, greater psoas muscle and smaller psoas muscle etc., divest vertebra, retain ridge dura mater, arachnoid and ridge mantle, and remove connective tissue and blood vessel, isolate spinal cord.When trend along nerve peels off nerve with distribution, carefully cut off muscle, remove connective tissue along the interstitial between muscle, peel off blood vessel, be separated the serous coat be coated on outside nerve, abundant NE complete separation is neural;
Step 6, by isolated ram nervous system nerves, rinse well with water, be placed in infuse container, then the ethanol water that mass percent is 80% is added, ethanol water is made not have ram nervous system nerves completely, soak two days later, taking-up water is rinsed well, picks clean residual muscle with scalpel; Again put back to infuse container, add absolute ethyl alcohol and dimethylbenzene mixed solution that volume ratio is 1:1 again, absolute ethyl alcohol and dimethylbenzene mixed solution is made not to have ram nervous system nerves completely, changed every 24 hours and once soak the absolute ethyl alcohol and dimethylbenzene mixed solution that volume ratio used is 1:1, soak and take out two days later; Or isolated ram nervous system nerves can be placed in infuse container, pour gasoline into, airtight, soak and carry out degreasing in about 7 days.In operating process, to obturage infuse container with Polypropylence Sheet, prevent solution evaporation, in order to avoid damage operating personnel;
Process after step 7, degreasing: the ram nerve after degreasing is placed on dark ventilation and dries in the air 7 days, makes neural residual dimethylbenzene volatilization in order to avoid damage operating personnel; Be the detergent solution immersion 2h of 5% again by the ram nerve mass percent dried well, then use clean water three times, thoroughly except removal xylene;
Step 8, by cleaning after ram nerve insert the hydrogen peroxide after dilution, soaks 5 days, when ram nerve becomes taking-up, rinse well with clear water, dry;
Step 9, with glue by neurofixation on a glass, make neural posture when keeping ram to stand, so that the displaying that sample is lively and active; Then put in specimen jar, namely prepare the present invention and to grow up the nervous system sample of ram.
embodiment 4
A nervous system Slide processing for adult ram, its concrete preparation process is:
Step one, the adult ram one selecting build plentiful and substantial, healthy and strong, claim its body weight;
Step 2, solution preparation: the body weight according to ram takes yellow Jackets, standard is that every 1kg goat takes yellow Jackets 45mg, is dissolved in stroke-physiological saline solution and is made into the Nembutal sodium solution that mass fraction is 3%, for subsequent use; The mass percent with distilled water formalin solution being diluted to formaldehyde is the solution of 7%, prepares the formalin solution after dilution, for subsequent use; The mass percent with distilled water hydrogen peroxide being diluted to hydrogen peroxide is the solution of 3%, prepares the hydrogen peroxide after dilution, for subsequent use; Take washing powder, add distilled water, prepare the detergent solution that mass percent is 3% ~ 5%; Prepared solution want matching while using or be placed in 4 DEG C for subsequent use;
Step 3, perfusion fixation: adopt mass percent to be that 10% carry out of chloraldurate aqueous solution lumbar injection 100ml to adult ram is anaesthetized.Then open at the skin place of ram jugular vein ditch the mouth that diameter is 5 cm with scalpel, expose arteria carotis communis; On arteria carotis communis, the osculum that diameter is 0.5cm is cut with operating scissors, perfusion syringe needle is inserted in osculum, fix with haemostatic clamp, quick filling 4000ml physiological saline, treat that blood is rinsed well, after namely liver bleaches, pour into the formalin solution after 5000 ml dilutions, first quick and back slow, about two hours are continued;
Step 4, peeling: ram corpse is faced upward and is put on autopsy table, being faced upward by ram corpse is immediately put on autopsy table, inside front wrist joint and hind leg tarsal joint, cutting the osculum that diameter is 1cm respectively with operating scissors, then inside osculum, cutting off ram skin Zhiyin side in front of the door respectively from cutting; Then be separated ram skin, then a people grabs two hind legs, and skin is sloughed to neck by the method for another people's drill drift, becomes dermatotome housing type outwardly; Cut off the auricular cartilage of ear again, be separated eye and mouth skin of lip, by complete for skin stripping, gill after finally isolating nervus visceralis;
Step 5, the neural nerve of separation ram: adopt bone saw to cut ram head, divest skull, retain brain dura mater, arachnoid and Pial matter, isolate brain, cerebellum; Reject chest, waist back of the body waist eye muscle, iliocostalis, head longissimus dorsi muscle, greater psoas muscle and smaller psoas muscle etc., divest vertebra, retain ridge dura mater, arachnoid and ridge mantle, and remove connective tissue and blood vessel, isolate spinal cord.When trend along nerve peels off nerve with distribution, carefully cut off muscle, remove connective tissue along the interstitial between muscle, peel off blood vessel, be separated the serous coat be coated on outside nerve, abundant NE complete separation is neural;
Step 6, by isolated ram nervous system nerves, to rinse well with water, be placed in infuse container, pour gasoline into, airtight, soak and carry out degreasing in about 7 days.In operating process, to obturage infuse container with Polypropylence Sheet, prevent solution evaporation, in order to avoid damage operating personnel;
Process after step 7, degreasing: the ram nerve after degreasing is placed on dark ventilation and dries in the air 7 days, makes neural residual dimethylbenzene volatilization in order to avoid damage operating personnel; Be the detergent solution immersion 2h of 5% again by the ram nerve mass percent dried well, then use clean water three times, thoroughly except removal xylene;
Step 8, by cleaning after ram nerve insert the hydrogen peroxide after dilution, soaks 5 days, when ram nerve bleaches taking-up, rinse well with clear water, dry;
Step 9, with glue by neurofixation on a glass, make neural posture when keeping ram to stand, so that the displaying that sample is lively and active; Then put in specimen jar, namely prepare the present invention and to grow up the nervous system sample of ram.
Claims (2)
1. a nervous system Slide processing for adult ram, is characterized in that: its concrete preparation process is:
Step one, solution preparation: the mass percent with distilled water hydrogen peroxide being diluted to hydrogen peroxide is the solution of 3%, prepares the hydrogen peroxide after dilution, for subsequent use; Take washing powder, add distilled water, prepare the detergent solution that mass percent is 3% ~ 5%; Prepared solution is wanted matching while using or is placed in 4 DEG C of preservations;
After step 2, selection anesthesia, through the corpse of the adult ram that perfusion fixation is put to death;
Step 3, peeling: faced upward by ram corpse and be put on autopsy table, cut the osculum that diameter is 0.5 ~ 1cm respectively with operating scissors inside front wrist joint and hind leg tarsal joint, inside cut osculum, then cut off ram skin Zhiyin side in front of the door respectively; Then be separated ram skin, by the method for drill drift, skin sloughed to neck, become dermatotome housing type outwardly; Cut off the auricular cartilage of ear again, be separated eye and mouth skin of lip, by complete for skin stripping, gill after finally isolating nervus visceralis;
Step 4, the neural nerve of separation ram: adopt bone saw to cut ram head, divest skull, retain brain dura mater, arachnoid and Pial matter, isolate brain, cerebellum, brain stem; Reject muscle, divest vertebra, retain ridge dura mater, arachnoid and ridge mantle, and remove connective tissue and blood vessel, isolate spinal cord; When peeling off neural along the trend of nerve and distribution, cut off muscle, remove connective tissue along the interstitial between muscle, peel off blood vessel, be separated the serous coat being coated on neural outside, expose ram nerve and by its complete separation;
Step 5, degreasing: by isolated ram nervous system nerves, rinse well with water, be placed in infuse container, then the ethanol water that mass percent is 80% is added, ethanol water is made not have ram nervous system nerves completely, soak two days later, taking-up water is rinsed well, picks clean residual muscle with scalpel; Again put back to infuse container, adding volume ratio is again the absolute ethyl alcohol of 1:1 and the mixed solution of dimethylbenzene, absolute ethyl alcohol and dimethylbenzene mixed solution is made not to have ram nervous system nerves completely, changed every 12 ~ 24 hours and once soak the absolute ethyl alcohol and dimethylbenzene mixed solution that volume ratio used is 1:1, soak and take out two days later; In the operating process of described degreasing, to obturage infuse container with Polypropylence Sheet, prevent solution evaporation;
Process after step 6, degreasing: the ram nerve after degreasing is placed on dark ventilation and dries in the air 5 ~ 7 days, then by the ram nerve mass percent dried well be 3% ~ 5% detergent solution soak 1 ~ 2h, then use clean water three times;
Step 7, above-mentioned steps six is processed after ram nerve insert the hydrogen peroxide after dilution, soaks 2 ~ 5 days, when ram nerve bleaches taking-up, rinse well with clear water, dry;
Step 8, with glue by neurofixation on a glass, make neural posture when keeping ram to stand, then put in specimen jar, namely prepare the nervous system sample of adult ram.
2. the nervous system Slide processing of a kind of adult ram as claimed in claim 1, it is characterized in that: the degreasing method in described step 5 adopts: isolated ram nervous system nerves is placed in infuse container, pour gasoline into, airtight, soak and carry out degreasing in 7 days.
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| CN103824496B (en) * | 2014-01-29 | 2015-10-28 | 河南科技大学 | A kind of preparation method of adult ram sample |
| CN103843757A (en) * | 2014-03-17 | 2014-06-11 | 内蒙古农业大学 | Method for manufacturing freeze-dried livestock specimen |
| CN105632313A (en) * | 2016-04-01 | 2016-06-01 | 昆山周庄鸿峰生命奥秘博物馆有限公司 | Preparation method of lizard specimen |
| CN106725973B (en) * | 2017-02-16 | 2020-05-01 | 临沂大学 | Craniotomy method for obtaining complete sheep brain |
| CN112146949B (en) * | 2020-08-19 | 2024-04-02 | 辽宁省海洋水产科学研究院 | Complete extraction method of stichopus japonicus selenka lime rings |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SU1711024A1 (en) * | 1990-02-20 | 1992-02-07 | Томский Медицинский Институт | Clearing agent for histologic nerve tissue preparations |
| CN101667352A (en) * | 2009-10-20 | 2010-03-10 | 中国人民解放军第二军医大学 | Method for preparing muscle preparation capable of displaying intramuscular nerves and blood vessel simultaneously |
| WO2012123492A1 (en) * | 2011-03-16 | 2012-09-20 | Universita' Degli Studi Di Cagliari | Method, composition and kit of visualization and characterization of the nervous system by combining the staining for metal impregnation and immunohistochemistry |
-
2013
- 2013-07-05 CN CN201310280718.6A patent/CN103380768B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SU1711024A1 (en) * | 1990-02-20 | 1992-02-07 | Томский Медицинский Институт | Clearing agent for histologic nerve tissue preparations |
| CN101667352A (en) * | 2009-10-20 | 2010-03-10 | 中国人民解放军第二军医大学 | Method for preparing muscle preparation capable of displaying intramuscular nerves and blood vessel simultaneously |
| WO2012123492A1 (en) * | 2011-03-16 | 2012-09-20 | Universita' Degli Studi Di Cagliari | Method, composition and kit of visualization and characterization of the nervous system by combining the staining for metal impregnation and immunohistochemistry |
Non-Patent Citations (4)
| Title |
|---|
| 作者,李仲购等.介绍一种神经系统全貌标本制作技术.《中国临床解剖学杂志》.2007,第25卷(第3期),第3和5页. * |
| 关贵武,等.脊椎动物神担标本制作法.《生物学通报》.1956,(第11期),54-58. * |
| 王荣林,等.动物教学干式标本的制作(五).《四川畜牧兽医》.2004,第31卷(第9期),59-60. * |
| 赵玉娥,等.动物透明标本的制作方法.《畜牧与饲料科学》.2012,第33卷(第10期),40. * |
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