CN103404506A - Method for preparing specimen of muscle of whole adult fox - Google Patents
Method for preparing specimen of muscle of whole adult fox Download PDFInfo
- Publication number
- CN103404506A CN103404506A CN2013102869854A CN201310286985A CN103404506A CN 103404506 A CN103404506 A CN 103404506A CN 2013102869854 A CN2013102869854 A CN 2013102869854A CN 201310286985 A CN201310286985 A CN 201310286985A CN 103404506 A CN103404506 A CN 103404506A
- Authority
- CN
- China
- Prior art keywords
- muscle
- fox
- whole
- skin
- adult
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Abstract
The invention discloses a method for preparing a specimen of muscle of a whole adult fox. The method comprises the steps of selecting a dead body of an anesthetized adult fox killed by perfusion fixation; then peeling the adult fox, completely separating the muscle of the whole fox; cleaning the separated muscle of the fox with water, soaking the muscle of the fox by using a 80 percent of alcohol water solution for two days, and then taking out the muscle of the fox, washing the muscle of the fox with water, soaking the muscle of the fox by using a mixed solution of absolute ethyl alcohol and dimethylbenzene with a volume ratio of 1.3:2 for two days and then taking the muscle of the fox out, airing the muscle of the fox in a dark ventilating place for 7-8 days, then soaking the muscle of the fox in an aqueous hydrogen peroxide solution with a mass percent of 4.4 percent for 8 days and then taking out the muscle of the fox, cleaning the muscle of the fox by using clear water, and airing; then fixing the muscle on a glass plate by using glue water to ensure that the muscle is kept in a gesture that the fox stands, then placing the muscle of the fox in a specimen jar to prepare the specimen of muscle of whole adult fox. According to the method disclosed by the invention, the preparation method is simple, and the cost is low; the prepared specimen of muscle of whole adult fox is easy to store, complete and attractive in structure, and clear and natural in form and position.
Description
Technical field
The present invention relates to a kind of Slide processing, specifically the whole-body muscle Slide processing of a kind of adult fox.
Background technology
Zoological specimens, be to keep former state in kind or process to arrange referential individuality that can be used as representative in one species in the time of can supplying study, research, also can be used as the important references of the universal publicity of scientific research, teaching and Sci-tech Knowledge.The scientific value can not be substituted, ornamental value and reserve value that animal and tissue specimen contain, other arts work of erect image equally enter into family, become the indoor new ornament of people.Make exquisite sample for one and not only bring people's knowledge, also bring simultaneously the enjoyment of people's art.Perfectly the animal muscle sample is the practical teaching aid of an image, can be again the art work of an exquisiteness, and it represents the development level of colleges and universities, a subject and grasps this subject researcher's professional skill.Preparation of specimen is also a research work, in manufacturing process, can constantly discover problems and solve them, and creates new technology, can broaden one's knowledge simultaneously, tempers participant's thinking and manipulative ability.Be made into whole muscle specimen, can demonstrate the whole-body muscle structure, the sample structure is attractive in appearance, form is known nature, not only " Zootomy " teaching in as directly perceived, vivid teaching aid, and in manufacturing process, can allow the student participate in, and allow the student understand and grasp preparation of specimen's process, cultivate simultaneously student's the ability of starting, beat one's brains.Help the understanding to animal whole-body muscle overview to the student, the contour structures of whole-body muscle is grasped, the understanding of the Name & Location relation of muscle is all played to good help, help to improve teaching efficiency.
Summary of the invention
The present invention seeks to as solving the problems of the technologies described above, the whole-body muscle Slide processing of a kind of adult fox is provided, its preparation method is simple, and prepared sample can demonstrate the whole-body muscle of the fox that grows up, and structure is attractive in appearance, and form is known nature.
The whole-body muscle Slide processing of a kind of adult fox, its concrete preparation process comprises:
After step 1, selection anesthesia, the corpse of the adult fox that the process perfusion fixation is put to death;
Step 2, peeling: the fox corpse is faced upward and is put on autopsy table, at front wrist joint and hind leg tarsal joint inboard, cut the osculum that diameter is 1.5 ~ 2cm respectively with operating scissors, then from cutting osculum inboard, cut off the fox skin skin to anus the place ahead respectively; Then separate the fox skin skin, by the method for drill drift, skin is sloughed to neck, make it become dermatotome housing type outwardly; Cut off again the auricular cartilage of ear, separate eye and lip section skin, peel off skin is complete, finally gill;
The whole-body muscle of step 3, separation fox: the muscle of isolating the fox whole body with scalpel; Concrete operation method is: peel off under head, neck and trunk skin and after fascia superficialis, isolate scalp flesh, musculus cutaneus colli and trunk musculus cutaneus.Along muscle distribution position, wipe out manadesma and connective tissue, isolate facial muscle, masseter and the temporalis of head; At neck portion from cucullaris, omoplate transverse process flesh, longest muscle of neck, arm head flesh, musculus sternocephalicus, splenius, semispinalis and musculus hyoideus.At trunk, after along boundary, the terminal of muscle, peeling off manadesma, connective tissue and blood vessel, separate the muscle such as flesh, latissimus dorsi, intercostal muscle, back of the body waist eye muscle, iliocostalis, greater psoas muscle, smaller psoas muscle, iliocostalis ditch, dorsal part serratus, external intercostal muscles, intercostals interni, musculus obliquus externus abdominis, oblique, rectus aabdominis, musculus trasversus abdomins on latissimus dorsi, chest muscle, veutro serratus, intercostal muscle, levator muscles of ribs, scalenus, musculus longus colli, omoplate.At forelimb, separate supraspinous muscle, infraspinous muscle, deltoid muscle, subscapular muscle, musculus teres major, arm flesh, antebrachial fascia tensor muscle, arm triceps, arm flesh, arm biceps, forearm, forward foot in a step flesh; At hind leg, separate gluteus, musculus glutaeus superficialis, gluteus medius, musculus glutaeus profundus, thigh section flesh, stern biceps muscle of thigh, semitendinosus, semimembranosus, tensor fasciae late muscle, quadriceps muscle of thigh, gracilis, adductor, shank and rear foot flesh etc.;
Step 4, degreasing: by isolated fox whole-body muscle, water is rinsed well, is placed in the infuse container, and then adding mass percent is 80% ethanol water, makes ethanol water not have fox muscle fully, soaks two days later, takes out water and rinses well; Again put back to the infuse container, adding volume ratio is the absolute ethyl alcohol of 1.3:2 and the mixed solution of dimethylbenzene again, make absolute ethyl alcohol and dimethylbenzene mixed solution not have fox muscle fully, every 16 hours, change and once soak absolute ethyl alcohol and the dimethylbenzene mixed solution that volume ratio used is 1.3:2, soak two days later and take out; Perhaps isolated fox whole-body muscle is placed in to the infuse container, pours gasoline into, airtight, soak and carried out degreasing in 4 days.In the operating process of degreasing,, with the Polypropylence Sheet infuse container of obturaging, prevent solution evaporation.
Processing after step 5, degreasing: the fox muscle after degreasing is placed on to dark ventilation and dried in the air 7～8 days, then the fox muscle that will dry well is that 6% detergent solution soaks 1～2h by mass percent, then clean three times with clear water;
It is 4.4% aqueous hydrogen peroxide solution that step 6, the fox muscle after above-mentioned steps five is processed are inserted mass percent, soaks taking-up afterwards in 8 days, rinses well with clear water, dries;
Step 7, with glue, muscle is fixed on glass plate, the posture while making muscle keep fox to stand, then put in specimen jar, namely prepares the whole-body muscle sample of the fox that grows up.
Beneficial effect is:
1, the whole-body muscle Slide processing of the adult fox of the present invention, simple, the easy row of preparation method, with low cost; Adopt ethanol water, absolute ethyl alcohol and dimethylbenzene or gasoline to carry out ungrease treatment to fox muscle, degreasing is thorough, can prevent from using and preserving permanent, and slowly the oozing out of fox muscle grease, pollutes mouldyly, makes sample impaired, hinders use and attractive in appearance.The mass percent that adopts hydrogen oxide is that after 4.4% dilution, hydrogen peroxide is bleached, and effect is better and to fox whole-body muscle sample not damaged.
2, the whole-body muscle Slide processing of the adult fox of the present invention, the whole-body muscle sample of prepared fox, be easy to preserve, structural integrity is attractive in appearance, morphologic localization is known nature, and whole-body muscle all clearly illustrated, each piece muscle all represents and be fixed on the physical slot of chicken body well.Solved the problem that is difficult to clear resolution fox whole-body muscle.Muscle is the emphasis in Teaching of Anatomy, and the muscle in the Systematic Anatomy teaching is mainly theoretical, and topologic Students in Teaching is not dissected whole muscle; The present invention preferably resolves this difficult problem.Excite again student's learning interest, improved teaching efficiency.
Embodiment
The whole-body muscle Slide processing of a kind of adult fox, its concrete preparation process comprises:
After step 1, selection anesthesia, the corpse of the adult fox that the process perfusion fixation is put to death;
Step 2, peeling: the fox corpse is faced upward and is put on autopsy table, at front wrist joint and hind leg tarsal joint inboard, cut the osculum that diameter is 1.5 ~ 2cm respectively with operating scissors, then from cutting osculum inboard, cut off the fox skin skin to anus the place ahead respectively; Then separate the fox skin skin, by the method for drill drift, skin is sloughed to neck, make it become dermatotome housing type outwardly; Cut off again the auricular cartilage of ear, separate eye and lip section skin, peel off skin is complete, finally gill;
The whole-body muscle of step 3, separation fox: the muscle of isolating the fox whole body with scalpel; Concrete operation method is: peel off under head, neck and trunk skin and after fascia superficialis, isolate scalp flesh, musculus cutaneus colli and trunk musculus cutaneus.Along muscle distribution position, wipe out manadesma and connective tissue, isolate facial muscle, masseter and the temporalis of head; At neck portion from cucullaris, omoplate transverse process flesh, longest muscle of neck, arm head flesh, musculus sternocephalicus, splenius, semispinalis and musculus hyoideus.At trunk, after along boundary, the terminal of muscle, peeling off manadesma, connective tissue and blood vessel, separate the muscle such as flesh, latissimus dorsi, intercostal muscle, back of the body waist eye muscle, iliocostalis, greater psoas muscle, smaller psoas muscle, iliocostalis ditch, dorsal part serratus, external intercostal muscles, intercostals interni, musculus obliquus externus abdominis, oblique, rectus aabdominis, musculus trasversus abdomins on latissimus dorsi, chest muscle, veutro serratus, intercostal muscle, levator muscles of ribs, scalenus, musculus longus colli, omoplate.At forelimb, separate supraspinous muscle, infraspinous muscle, deltoid muscle, subscapular muscle, musculus teres major, arm flesh, antebrachial fascia tensor muscle, arm triceps, arm flesh, arm biceps, forearm, forward foot in a step flesh; At hind leg, separate gluteus, musculus glutaeus superficialis, gluteus medius, musculus glutaeus profundus, thigh section flesh, stern biceps muscle of thigh, semitendinosus, semimembranosus, tensor fasciae late muscle, quadriceps muscle of thigh, gracilis, adductor, shank and rear foot flesh etc.;
Step 4, degreasing: by isolated fox whole-body muscle, water is rinsed well, is placed in the infuse container, and then adding mass percent is 80% ethanol water, makes ethanol water not have fox muscle fully, soaks two days later, takes out water and rinses well; Again put back to the infuse container, adding volume ratio is the absolute ethyl alcohol of 1.3:2 and the mixed solution of dimethylbenzene again, make absolute ethyl alcohol and dimethylbenzene mixed solution not have fox muscle fully, every 16 hours, change and once soak absolute ethyl alcohol and the dimethylbenzene mixed solution that volume ratio used is 1.3:2, soak two days later and take out; Perhaps isolated fox whole-body muscle is placed in to the infuse container, pours gasoline into, airtight, soak and carried out degreasing in 4 days.In the operating process of degreasing,, with the Polypropylence Sheet infuse container of obturaging, prevent solution evaporation.
Processing after step 5, degreasing: the fox muscle after degreasing is placed on to dark ventilation and dried in the air 7～8 days, then the fox muscle that will dry well is that 6% detergent solution soaks 1～2h by mass percent, then clean three times with clear water;
It is 4.4% aqueous hydrogen peroxide solution that step 6, the fox muscle after above-mentioned steps five is processed are inserted mass percent, soaks taking-up afterwards in 8 days, rinses well with clear water, dries;
Step 7, with glue, muscle is fixed on glass plate, the posture while making muscle keep fox to stand, then put in specimen jar, namely prepares the whole-body muscle sample of the fox that grows up.
Following examples can make the present invention of those skilled in the art's complete understanding, but do not limit the present invention in any way.
Embodiment 1
The whole-body muscle Slide processing of a kind of adult fox, its concrete preparation process comprises:
Step 1, select one of plentiful and substantial, the healthy and strong adult fox of build, claim its body weight;
Step 2, solution preparation: according to the body weight of fox, take yellow Jackets, standard is that every 1kg fox takes yellow Jackets 45mg, and it is dissolved in to stroke-physiological saline solution, and to be made into mass fraction be 3% amobarbital sodium solution, standby; By the mass percent that distilled water is diluted to formaldehyde by formalin solution, be 7% solution, prepare the formalin solution after dilution, standby;
Step 3, perfusion fixation: with syringe, draw the prepared amobarbital sodium solution of above-mentioned steps two, adopt intraperitoneal injection anesthesia fox; Then with scalpel, opening diameter at the skin place of fox jugular vein ditch is the mouth of 3 cm, exposes arteria carotis communis; With operating scissors, cut the osculum that diameter is 0.3cm on arteria carotis communis, in osculum, insert the perfusion syringe needle, with haemostatic clamp, fix, quick filling 3000ml physiological saline, treat that blood rinses well, after namely liver bleaches, the formalin solution after perfusion 5000 ml dilutions, first quick and back slow, continue approximately two hours;
Step 4, peeling: the fox corpse is faced upward and is put on autopsy table, at front wrist joint and hind leg tarsal joint inboard, cut the osculum that diameter is 1.5cm respectively with operating scissors, then from cutting osculum inboard, cut off the fox skin skin to anus the place ahead respectively; Then separate the fox skin skin, by the method for drill drift, skin is sloughed to neck, make it become dermatotome housing type outwardly; Cut off again the auricular cartilage of ear, separate eye and lip section skin, peel off skin is complete, finally gill;
The whole-body muscle of step 5, separation fox: the muscle of isolating the fox whole body with scalpel; Concrete operation method is: peel off under head, neck and trunk skin and after fascia superficialis, isolate scalp flesh, musculus cutaneus colli and trunk musculus cutaneus.Along muscle distribution position, wipe out manadesma and connective tissue, isolate facial muscle, masseter and the temporalis of head; At neck portion from cucullaris, omoplate transverse process flesh, longest muscle of neck, arm head flesh, musculus sternocephalicus, splenius, semispinalis and musculus hyoideus.At trunk, after along boundary, the terminal of muscle, peeling off manadesma, connective tissue and blood vessel, separate the muscle such as flesh, latissimus dorsi, intercostal muscle, back of the body waist eye muscle, iliocostalis, greater psoas muscle, smaller psoas muscle, iliocostalis ditch, dorsal part serratus, external intercostal muscles, intercostals interni, musculus obliquus externus abdominis, oblique, rectus aabdominis, musculus trasversus abdomins on latissimus dorsi, chest muscle, veutro serratus, intercostal muscle, levator muscles of ribs, scalenus, musculus longus colli, omoplate.At forelimb, separate supraspinous muscle, infraspinous muscle, deltoid muscle, subscapular muscle, musculus teres major, arm flesh, antebrachial fascia tensor muscle, arm triceps, arm flesh, arm biceps, forearm, forward foot in a step flesh; At hind leg, separate gluteus, musculus glutaeus superficialis, gluteus medius, musculus glutaeus profundus, thigh section flesh, stern biceps muscle of thigh, semitendinosus, semimembranosus, tensor fasciae late muscle, quadriceps muscle of thigh, gracilis, adductor, shank and rear foot flesh etc.;
Step 6, degreasing: by isolated fox whole-body muscle, water is rinsed well, is placed in the infuse container, and then adding mass percent is 80% ethanol water, makes ethanol water not have fox muscle fully, soaks two days later, takes out water and rinses well; Again put back to the infuse container, adding volume ratio is the absolute ethyl alcohol of 1.3:2 and the mixed solution of dimethylbenzene again, make absolute ethyl alcohol and dimethylbenzene mixed solution not have fox muscle fully, every 16 hours, change and once soak absolute ethyl alcohol and the dimethylbenzene mixed solution that volume ratio used is 1.3:2, soak two days later and take out; In the operating process of degreasing,, with the Polypropylence Sheet infuse container of obturaging, prevent solution evaporation.
Processing after step 7, degreasing: the fox muscle after degreasing is placed on to dark ventilation and dried in the air 7 days, then the fox muscle that will dry well is that 6% detergent solution soaks 1h by mass percent, then clean three times with clear water;
It is 4.4% aqueous hydrogen peroxide solution that step 8, the fox muscle after above-mentioned steps five is processed are inserted mass percent, soaks taking-up afterwards in 8 days, rinses well with clear water, dries;
Step 9, with glue, muscle is fixed on glass plate, the posture while making muscle keep fox to stand, then put in specimen jar, namely prepares the whole-body muscle sample of the fox that grows up.
Embodiment 2
The whole-body muscle Slide processing of a kind of adult fox, its concrete preparation process comprises:
Step 1, select one of plentiful and substantial, the healthy and strong adult fox of build, claim its body weight;
Step 2, solution preparation: according to the body weight of fox, take yellow Jackets, standard is that every 1kg fox takes yellow Jackets 45mg, and it is dissolved in to stroke-physiological saline solution, and to be made into mass fraction be 3% amobarbital sodium solution, standby; By the mass percent that distilled water is diluted to formaldehyde by formalin solution, be 7% solution, prepare the formalin solution after dilution, standby;
Step 3, perfusion fixation: with syringe, draw the prepared amobarbital sodium solution of above-mentioned steps two, adopt intraperitoneal injection anesthesia fox; Then with scalpel, opening diameter at the skin place of fox jugular vein ditch is the mouth of 3 cm, exposes arteria carotis communis; With operating scissors, cut the osculum that diameter is 0.3cm on arteria carotis communis, in osculum, insert the perfusion syringe needle, with haemostatic clamp, fix, quick filling 3000ml physiological saline, treat that blood rinses well, after namely liver bleaches, the formalin solution after perfusion 5000 ml dilutions, first quick and back slow, continue approximately two hours;
Step 4, peeling: the fox corpse is faced upward and is put on autopsy table, at front wrist joint and hind leg tarsal joint inboard, cut the osculum that diameter is 2cm respectively with operating scissors, then from cutting osculum inboard, cut off the fox skin skin to anus the place ahead respectively; Then separate the fox skin skin, by the method for drill drift, skin is sloughed to neck, make it become dermatotome housing type outwardly; Cut off again the auricular cartilage of ear, separate eye and lip section skin, peel off skin is complete, finally gill;
The whole-body muscle of step 5, separation fox: the muscle of isolating the fox whole body with scalpel; Concrete operation method is: peel off under head, neck and trunk skin and after fascia superficialis, isolate scalp flesh, musculus cutaneus colli and trunk musculus cutaneus.Along muscle distribution position, wipe out manadesma and connective tissue, isolate facial muscle, masseter and the temporalis of head; At neck portion from cucullaris, omoplate transverse process flesh, longest muscle of neck, arm head flesh, musculus sternocephalicus, splenius, semispinalis and musculus hyoideus.At trunk, after along boundary, the terminal of muscle, peeling off manadesma, connective tissue and blood vessel, separate the muscle such as flesh, latissimus dorsi, intercostal muscle, back of the body waist eye muscle, iliocostalis, greater psoas muscle, smaller psoas muscle, iliocostalis ditch, dorsal part serratus, external intercostal muscles, intercostals interni, musculus obliquus externus abdominis, oblique, rectus aabdominis, musculus trasversus abdomins on latissimus dorsi, chest muscle, veutro serratus, intercostal muscle, levator muscles of ribs, scalenus, musculus longus colli, omoplate.At forelimb, separate supraspinous muscle, infraspinous muscle, deltoid muscle, subscapular muscle, musculus teres major, arm flesh, antebrachial fascia tensor muscle, arm triceps, arm flesh, arm biceps, forearm, forward foot in a step flesh; At hind leg, separate gluteus, musculus glutaeus superficialis, gluteus medius, musculus glutaeus profundus, thigh section flesh, stern biceps muscle of thigh, semitendinosus, semimembranosus, tensor fasciae late muscle, quadriceps muscle of thigh, gracilis, adductor, shank and rear foot flesh etc.;
Step 6, degreasing: by isolated fox whole-body muscle, water is rinsed well, is placed in the infuse container, and then adding mass percent is 80% ethanol water, makes ethanol water not have fox muscle fully, soaks two days later, takes out water and rinses well; Again put back to the infuse container, adding volume ratio is the absolute ethyl alcohol of 1.3:2 and the mixed solution of dimethylbenzene again, make absolute ethyl alcohol and dimethylbenzene mixed solution not have fox muscle fully, every 16 hours, change and once soak absolute ethyl alcohol and the dimethylbenzene mixed solution that volume ratio used is 1.3:2, soak two days later and take out; In the operating process of degreasing,, with the Polypropylence Sheet infuse container of obturaging, prevent solution evaporation.
Processing after step 7, degreasing: the fox muscle after degreasing is placed on to dark ventilation and dried in the air 8 days, then the fox muscle that will dry well is that 6% detergent solution soaks 2h by mass percent, then clean three times with clear water;
It is 4.4% aqueous hydrogen peroxide solution that step 8, the fox muscle after above-mentioned steps five is processed are inserted mass percent, soaks taking-up afterwards in 8 days, rinses well with clear water, dries;
Step 9, with glue, muscle is fixed on glass plate, the posture while making muscle keep fox to stand, then put in specimen jar, namely prepares the whole-body muscle sample of the fox that grows up.
Embodiment 3
The whole-body muscle Slide processing of a kind of adult fox, its concrete preparation process comprises:
Step 1, select one of plentiful and substantial, the healthy and strong adult fox of build, claim its body weight;
Step 2, solution preparation: according to the body weight of fox, take yellow Jackets, standard is that every 1kg fox takes yellow Jackets 45mg, and it is dissolved in to stroke-physiological saline solution, and to be made into mass fraction be 3% amobarbital sodium solution, standby; By the mass percent that distilled water is diluted to formaldehyde by formalin solution, be 7% solution, prepare the formalin solution after dilution, standby;
Step 3, perfusion fixation: with syringe, draw the prepared amobarbital sodium solution of above-mentioned steps two, adopt intraperitoneal injection anesthesia fox; Then with scalpel, opening diameter at the skin place of fox jugular vein ditch is the mouth of 3 cm, exposes arteria carotis communis; With operating scissors, cut the osculum that diameter is 0.3cm on arteria carotis communis, in osculum, insert the perfusion syringe needle, with haemostatic clamp, fix, quick filling 3000ml physiological saline, treat that blood rinses well, after namely liver bleaches, the formalin solution after perfusion 5000 ml dilutions, first quick and back slow, continue approximately two hours;
Step 4, peeling: the fox corpse is faced upward and is put on autopsy table, at front wrist joint and hind leg tarsal joint inboard, cut the osculum that diameter is 1.8cm respectively with operating scissors, then from cutting osculum inboard, cut off the fox skin skin to anus the place ahead respectively; Then separate the fox skin skin, by the method for drill drift, skin is sloughed to neck, make it become dermatotome housing type outwardly; Cut off again the auricular cartilage of ear, separate eye and lip section skin, peel off skin is complete, finally gill;
The whole-body muscle of step 5, separation fox: the muscle of isolating the fox whole body with scalpel; Concrete operation method is: peel off under head, neck and trunk skin and after fascia superficialis, isolate scalp flesh, musculus cutaneus colli and trunk musculus cutaneus.Along muscle distribution position, wipe out manadesma and connective tissue, isolate facial muscle, masseter and the temporalis of head; At neck portion from cucullaris, omoplate transverse process flesh, longest muscle of neck, arm head flesh, musculus sternocephalicus, splenius, semispinalis and musculus hyoideus.At trunk, after along boundary, the terminal of muscle, peeling off manadesma, connective tissue and blood vessel, separate the muscle such as flesh, latissimus dorsi, intercostal muscle, back of the body waist eye muscle, iliocostalis, greater psoas muscle, smaller psoas muscle, iliocostalis ditch, dorsal part serratus, external intercostal muscles, intercostals interni, musculus obliquus externus abdominis, oblique, rectus aabdominis, musculus trasversus abdomins on latissimus dorsi, chest muscle, veutro serratus, intercostal muscle, levator muscles of ribs, scalenus, musculus longus colli, omoplate.At forelimb, separate supraspinous muscle, infraspinous muscle, deltoid muscle, subscapular muscle, musculus teres major, arm flesh, antebrachial fascia tensor muscle, arm triceps, arm flesh, arm biceps, forearm, forward foot in a step flesh; At hind leg, separate gluteus, musculus glutaeus superficialis, gluteus medius, musculus glutaeus profundus, thigh section flesh, stern biceps muscle of thigh, semitendinosus, semimembranosus, tensor fasciae late muscle, quadriceps muscle of thigh, gracilis, adductor, shank and rear foot flesh etc.;
Step 6, degreasing: by isolated fox whole-body muscle, water is rinsed well, is placed in the infuse container, and then adding mass percent is 80% ethanol water, makes ethanol water not have fox muscle fully, soaks two days later, takes out water and rinses well; Again put back to the infuse container, adding volume ratio is the absolute ethyl alcohol of 1.3:2 and the mixed solution of dimethylbenzene again, make absolute ethyl alcohol and dimethylbenzene mixed solution not have fox muscle fully, every 16 hours, change and once soak absolute ethyl alcohol and the dimethylbenzene mixed solution that volume ratio used is 1.3:2, soak two days later and take out; In the operating process of degreasing,, with the Polypropylence Sheet infuse container of obturaging, prevent solution evaporation.
Processing after step 7, degreasing: the fox muscle after degreasing is placed on to dark ventilation and dried in the air 7 days, then the fox muscle that will dry well is that 6% detergent solution soaks 1.5h by mass percent, then clean three times with clear water;
It is 4.4% aqueous hydrogen peroxide solution that step 8, the fox muscle after above-mentioned steps five is processed are inserted mass percent, soaks taking-up afterwards in 8 days, rinses well with clear water, dries;
Step 9, with glue, muscle is fixed on glass plate, the posture while making muscle keep fox to stand, then put in specimen jar, namely prepares the whole-body muscle sample of the fox that grows up.
Embodiment 4
The whole-body muscle Slide processing of a kind of adult fox, its concrete preparation process comprises:
Step 1, select one of plentiful and substantial, the healthy and strong adult fox of build, claim its body weight;
Step 2, solution preparation: according to the body weight of fox, take yellow Jackets, standard is that every 1kg fox takes yellow Jackets 45mg, and it is dissolved in to stroke-physiological saline solution, and to be made into mass fraction be 3% amobarbital sodium solution, standby; By the mass percent that distilled water is diluted to formaldehyde by formalin solution, be 7% solution, prepare the formalin solution after dilution, standby;
Step 3, perfusion fixation: with syringe, draw the prepared amobarbital sodium solution of above-mentioned steps two, adopt intraperitoneal injection anesthesia fox; Then with scalpel, opening diameter at the skin place of fox jugular vein ditch is the mouth of 3 cm, exposes arteria carotis communis; With operating scissors, cut the osculum that diameter is 0.3cm on arteria carotis communis, in osculum, insert the perfusion syringe needle, with haemostatic clamp, fix, quick filling 3000ml physiological saline, treat that blood rinses well, after namely liver bleaches, the formalin solution after perfusion 5000 ml dilutions, first quick and back slow, continue approximately two hours;
Step 4, peeling: the fox corpse is faced upward and is put on autopsy table, at front wrist joint and hind leg tarsal joint inboard, cut the osculum that diameter is 1.7cm respectively with operating scissors, then from cutting osculum inboard, cut off the fox skin skin to anus the place ahead respectively; Then separate the fox skin skin, by the method for drill drift, skin is sloughed to neck, make it become dermatotome housing type outwardly; Cut off again the auricular cartilage of ear, separate eye and lip section skin, peel off skin is complete, finally gill;
The whole-body muscle of step 5, separation fox: the muscle of isolating the fox whole body with scalpel; Concrete operation method is: peel off under head, neck and trunk skin and after fascia superficialis, isolate scalp flesh, musculus cutaneus colli and trunk musculus cutaneus.Along muscle distribution position, wipe out manadesma and connective tissue, isolate facial muscle, masseter and the temporalis of head; At neck portion from cucullaris, omoplate transverse process flesh, longest muscle of neck, arm head flesh, musculus sternocephalicus, splenius, semispinalis and musculus hyoideus.At trunk, after along boundary, the terminal of muscle, peeling off manadesma, connective tissue and blood vessel, separate the muscle such as flesh, latissimus dorsi, intercostal muscle, back of the body waist eye muscle, iliocostalis, greater psoas muscle, smaller psoas muscle, iliocostalis ditch, dorsal part serratus, external intercostal muscles, intercostals interni, musculus obliquus externus abdominis, oblique, rectus aabdominis, musculus trasversus abdomins on latissimus dorsi, chest muscle, veutro serratus, intercostal muscle, levator muscles of ribs, scalenus, musculus longus colli, omoplate.At forelimb, separate supraspinous muscle, infraspinous muscle, deltoid muscle, subscapular muscle, musculus teres major, arm flesh, antebrachial fascia tensor muscle, arm triceps, arm flesh, arm biceps, forearm, forward foot in a step flesh; At hind leg, separate gluteus, musculus glutaeus superficialis, gluteus medius, musculus glutaeus profundus, thigh section flesh, stern biceps muscle of thigh, semitendinosus, semimembranosus, tensor fasciae late muscle, quadriceps muscle of thigh, gracilis, adductor, shank and rear foot flesh etc.;
Step 6, degreasing: by isolated fox whole-body muscle, water is rinsed well, is placed in the infuse container, pours gasoline into, airtight, soaks and carries out degreasing in 4 days.In the operating process of degreasing,, with the Polypropylence Sheet infuse container of obturaging, prevent solution evaporation.
Processing after step 7, degreasing: the fox muscle after degreasing is placed on to dark ventilation and dried in the air 8 days, then the fox muscle that will dry well is that 6% detergent solution soaks 2h by mass percent, then clean three times with clear water;
It is 4.4% aqueous hydrogen peroxide solution that step 8, the fox muscle after above-mentioned steps five is processed are inserted mass percent, soaks taking-up afterwards in 8 days, rinses well with clear water, dries;
Step 9, with glue, muscle is fixed on glass plate, the posture while making muscle keep fox to stand, then put in specimen jar, namely prepares the whole-body muscle sample of the fox that grows up.
Claims (3)
1. the whole-body muscle Slide processing of an adult fox, it is characterized in that: its concrete preparation process comprises:
After step 1, selection anesthesia, the corpse of the adult fox that the process perfusion fixation is put to death;
Step 2, peeling: the fox corpse is faced upward and is put on autopsy table, at front wrist joint and hind leg tarsal joint inboard, cut the osculum that diameter is 1.5～2cm respectively with operating scissors, then from cutting osculum inboard, cut off the fox skin skin to anus the place ahead respectively; Then separate the fox skin skin, by the method for drill drift, skin is sloughed to neck, make it become dermatotome housing type outwardly; Cut off again the auricular cartilage of ear, separate eye and lip section skin, peel off skin is complete, finally gill;
The whole-body muscle of step 3, separation fox: the muscle of isolating the fox whole body with scalpel;
Step 4, degreasing: by isolated fox muscle specimen, water is rinsed well, is placed in the infuse container, and then adding mass percent is 80% ethanol water, makes ethanol water not have fox muscle fully, soaks two days later, takes out water and rinses well; Again put back to the infuse container, adding volume ratio is the absolute ethyl alcohol of 1.3:2 and the mixed solution of dimethylbenzene again, make absolute ethyl alcohol and dimethylbenzene mixed solution not have fox muscle fully, every 16 hours, change and once soak absolute ethyl alcohol and the dimethylbenzene mixed solution that volume ratio used is 1.3:2, soak two days later and take out;
Processing after step 5, degreasing: the fox muscle after degreasing is placed on to dark ventilation and dried in the air 7～8 days, then the fox muscle that will dry well is that 6% detergent solution soaks 1～2h by mass percent, then clean three times with clear water;
It is 4.4% aqueous hydrogen peroxide solution that step 6, the fox muscle after above-mentioned steps five is processed are inserted mass percent, soaks taking-up afterwards in 8 days, rinses well with clear water, dries;
Step 7, with glue, muscle is fixed on glass plate, the posture while making muscle keep fox to stand, then put in specimen jar, namely prepares the whole-body muscle sample of the fox that grows up.
2. the whole-body muscle Slide processing of a kind of adult fox as claimed in claim 1 is characterized in that: in the operating process of described step 4 degreasing,, with the Polypropylence Sheet infuse container of obturaging, prevent solution evaporation.
3. the whole-body muscle Slide processing of a kind of adult fox as claimed in claim 1, it is characterized in that: the degreasing method in described step 4 also can adopt: isolated fox muscle specimen is placed in to the infuse container, pour gasoline into, airtight, soak and carried out degreasing in 4 days.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310286985.4A CN103404506B (en) | 2013-07-10 | 2013-07-10 | Method for preparing specimen of muscle of whole adult fox |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310286985.4A CN103404506B (en) | 2013-07-10 | 2013-07-10 | Method for preparing specimen of muscle of whole adult fox |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103404506A true CN103404506A (en) | 2013-11-27 |
| CN103404506B CN103404506B (en) | 2015-05-13 |
Family
ID=49597596
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310286985.4A Expired - Fee Related CN103404506B (en) | 2013-07-10 | 2013-07-10 | Method for preparing specimen of muscle of whole adult fox |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103404506B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103824497A (en) * | 2014-01-29 | 2014-05-28 | 河南科技大学 | Manufacturing method of adult male fox specimen |
| CN112419856A (en) * | 2020-11-30 | 2021-02-26 | 河南中博生物塑化科技有限公司 | Preparation method of specimen for cardiac interventional operation |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1971663A (en) * | 2006-12-06 | 2007-05-30 | 王荣林 | Dry type manufacturing method of animal specimen |
| US20080108043A1 (en) * | 2006-11-08 | 2008-05-08 | Richard-Allan Scientific Company | Rapid tissue processing system |
| CN101667352A (en) * | 2009-10-20 | 2010-03-10 | 中国人民解放军第二军医大学 | Method for preparing muscle preparation capable of displaying intramuscular nerves and blood vessel simultaneously |
-
2013
- 2013-07-10 CN CN201310286985.4A patent/CN103404506B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20080108043A1 (en) * | 2006-11-08 | 2008-05-08 | Richard-Allan Scientific Company | Rapid tissue processing system |
| CN1971663A (en) * | 2006-12-06 | 2007-05-30 | 王荣林 | Dry type manufacturing method of animal specimen |
| CN101667352A (en) * | 2009-10-20 | 2010-03-10 | 中国人民解放军第二军医大学 | Method for preparing muscle preparation capable of displaying intramuscular nerves and blood vessel simultaneously |
Non-Patent Citations (2)
| Title |
|---|
| 李仲购 等: "介绍一种神经系统全貌标本制作技术", 《中国临床解剖学杂志》 * |
| 李清滨: "肌肉石蜡干燥标本制作法", 《解剖学杂志》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103824497A (en) * | 2014-01-29 | 2014-05-28 | 河南科技大学 | Manufacturing method of adult male fox specimen |
| CN103824497B (en) * | 2014-01-29 | 2015-10-28 | 河南科技大学 | A kind of preparation method of adult dog fox sample |
| CN112419856A (en) * | 2020-11-30 | 2021-02-26 | 河南中博生物塑化科技有限公司 | Preparation method of specimen for cardiac interventional operation |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103404506B (en) | 2015-05-13 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102499231B (en) | Harmless fabrication method of sturgeon taxidermy specimen | |
| Vogel | Prime mover: a natural history of muscle | |
| CN103430934B (en) | Manufacturing method of adult cat musclespecimen | |
| CN103426354A (en) | Method for preparing whole-body muscle specimen of adult dog | |
| CN103400529B (en) | A kind of whole-body muscle Slide processing of mature rooster | |
| CN102945638B (en) | The manufacture method of Neomeris phocaenoides G. Cuvier skinned-specimen | |
| CN105973663B (en) | A kind of preparation method of colorful skeleton specimen | |
| CN103380768B (en) | Manufacturing method of adult ram nervous system specimen | |
| CN103404506B (en) | Method for preparing specimen of muscle of whole adult fox | |
| CN103824499B (en) | A kind of preparation method of adult rabbits sample | |
| CN103426355B (en) | The whole-body muscle Slide processing of a kind of adult otter | |
| CN103824497B (en) | A kind of preparation method of adult dog fox sample | |
| Müller et al. | Traditional and modern biomedical prospecting: Part I—the history | |
| CN103500526A (en) | Method for preparing muscle specimen of adult rabbit | |
| Wilson | The transformation of ancient concepts of respiration in the seventeenth century | |
| CN103824496B (en) | A kind of preparation method of adult ram sample | |
| CN103814888B (en) | Method for making adult cat specimen | |
| CN204990968U (en) | Kind of duck polychrome internal organs skeleton dry preserved specimen of spreading wings | |
| CN105355127B (en) | A kind of adult rat tail vein injection model production method based on mathematical modeling | |
| CN103824495B (en) | A kind of preparation method of adult otter sample | |
| CN103824498B (en) | A kind of preparation method of adult duck sample | |
| CN105145544A (en) | Method for making transparent parusvenustulus skeleton specimen | |
| CN103814889B (en) | A kind of preparation method of adult public dog sample | |
| CN105104358A (en) | Method for making transparent skeleton specimen of neonate rabbit | |
| CN107274770A (en) | A kind of preparation method of crystal heart efferent tract sample |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20150513 Termination date: 20160710 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |