CN105145544A - Method for making transparent parusvenustulus skeleton specimen - Google Patents
Method for making transparent parusvenustulus skeleton specimen Download PDFInfo
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Abstract
The invention relates to a method for making a transparent parusvenustulus skeleton specimen. Compared with the prior art, the dehydration gradient is increased, the gradient difference is reduced, dehydration is soft in intensity and thorough, and deformation or denaturation of the specimen can be prevented in the dehydration process. According to the method, sodium hydroxide and xylene are combined for degreasing, the degreasing effect is better and more thorough, degreasing time is short, specimen making time is shortened to a certain degree, and making efficiency is improved; a method that two kinds of transparency agents including potassium hydroxide and glycerin are used in cooperation is adopted, transparency is carried out for two days through a low-concentration potassium hydroxide solution and is soft, the specimen is not damaged, then the specimen is transferred into glycerin potassium hydroxide transparency liquid, and transparent treatment is carried out for 5 days; due to glycerin, corrosion of the specimen by potassium hydroxide is reduced, the concentration of the potassium hydroxide can be increased to be higher than 3.5%, and the transparency effect can be improved; as alcian blue dye liquor is used, cartilage is dyed into a blue color, and the transparent skeleton specimen is bright in color and more attractive.
Description
Technical field
The present invention relates to Slide processing technical field, be specifically related to a kind of yellow abdomen titmouse pattern of glassy carcass preparation method.
Background technology
Yellow abdomen titmouse formal name used at school is Parusvenustulus, and build is less, cock head and upper back of the body black, cheek and collare each tool one white blocks spot, lower back, waist light blue grey, and lower breast is yellow to lower tall coverts.Hen upper body celadon, chin, larynx, cheek and auricular canescence, the faint yellow green of all the other lower bodies.Yellow abdomen titmouse is special product of China birds, is distributed in the Gansu, China west and south, Mount Taibai, the Southern Shaanxi Qinling Mountains, the ground such as Mt. Funiushan In Henan Province.These species have been listed in " the terrestrial wildlife register that is useful or that have Important Economic, scientific research value of state guarantee " issued in China Forest office on August 1st, 2000.
Transparent specimen, can show and be difficult to observe clearly interior with general anatomic method.It when keeping sample profile complete, can demonstrate the endoskeletal POF relation of body and skeleton development situation.Be widely used in the teaching of the specialties such as medical anatomy, biology, zoology, Bone Development for Animals.Its producing principle is by chemistry and the method for physics, is first fixed the bone of animal, dyes, then make the index of refraction of soft tissue and the close of clarifier, reach soft tissue transparent, and makes that bone tissue is clear to be manifested.Pattern of glassy carcass not only enlivens classroom atmosphere as directly perceived, vivid teaching aid in the teaching such as " zoology ", " Zootomy ", " animal iconography ", strengthen the perception of student, and students' participation can be allowed in manufacturing process, allow student understand and grasp preparation of specimen's process, simultaneously the starting of training student, beat one's brains ability.In scientific research, animal skeleton specimen can not be subject to the restriction in time, place, for scientific research provides research material.In popular science propaganda, by the display and exhibition of animal skeleton specimen, strengthen people to the understanding of wildlife, be conducive to the protection work strengthening rare and endangered animal.Therefore, for education and scientific research person, the technology grasping animal skeleton specimen making is significant.
At present, pattern of glassy carcass preparation method generally comprises following steps: draw materials, fix, dewater, degreasing, transparent, dyeing, decolouring and preserve.Wherein usually adopt 80% ethanol, 90% ethanol and absolute ethyl alcohol by low concentration to high concentration to sample serial dehydration, after dehydration, distortion is serious.It is potassium hydroxide or sodium hydroxide that transparent processing commonly uses clarifier, potassium hydroxide and sodium hydroxide transparent effect good, but corrosivity is strong, when concentration more than 3.5% or clearing time is long time, easily make the embrittlement of sample bone, lose due color and luster and integrality, cause and make unsuccessfully.
Summary of the invention
The object of the present invention is to provide the preparation method of a kind of safety non-toxic, the yellow abdomen titmouse pattern of glassy carcass that with low cost, transparent effect is good.
For solving the problems of the technologies described above, the technical solution adopted in the present invention is: a kind of yellow abdomen titmouse pattern of glassy carcass preparation method, comprises the following steps:
Step one, get dead yellow abdomen titmouse process after with 4% formalin or absolute ethyl alcohol fix 10 days;
Step 2,50% ethanol, 70% ethanol, 80% ethanol, 85% ethanol, 90% ethanol, 95% ethanol and absolute ethyl alcohol is adopted to carry out serial dehydration, each concentration gradient 7 day to high concentration to the yellow abdomen titmouse after the fixing process of step one by low concentration;
Step 3, by the yellow abdomen titmouse after dehydration in mass concentration be 1% sodium hydroxide solution in degreasing 3-4 days, then use dimethylbenzene degreasing 1 day;
Step 4, the yellow abdomen titmouse of step 3 degreasing put into the potassium hydroxide solution transparent processing 2 days that mass concentration is 1%, to transfer in glycerine potassium hydroxide transparent liquid transparent processing afterwards 5 days, in described glycerine potassium hydroxide transparent liquid, the mass concentration of potassium hydroxide is 4.5%, mass percent shared by glycerine is 40%, and all the other are water;
Step 5, with Alizarin red staining liquid and A Li Xinlan dyeing liquor, the yellow abdomen titmouse after transparent processing to be dyeed successively, each dyeing 7 days;
Step 6, with 1% potassium hydroxide solution or 20% ammonium glycerin decoloring agent, the yellow abdomen titmouse sample after dyeing to be decoloured 5 days, make muscle be water white transparency, obtain yellow abdomen titmouse pattern of glassy carcass.
Further improvement, with the yellow abdomen titmouse that running water attitude is fixing before step 2 dehydration, then bleaches 7 days with 5% hydrogenperoxide steam generator or 1% potassium hydroxide solution.
Further improvement, Alizarin red staining liquid described in step 5 is the alizarin red dye liquor of the alizarin red ethanol dye liquor of 2% or 0.04% containing 2% potassium hydroxide; Described A Li Xinlan dyeing liquor is formed by the proportions containing 70% ethanolic solution of 0.2% A Li Xinlan, 70% ethanolic solution and glacial acetic acid 1:18:1 by volume.
Further improvement, yellow abdomen titmouse pattern of glassy carcass step 6 obtained dewaters with 20% glycerite, 30% glycerite, 40% glycerite, 50% glycerite, 60% glycerite, 70% glycerite and 100% glycerine gradient successively.
Compared with prior art, the invention has the beneficial effects as follows: the present invention adopts 50% ethanol, 70% ethanol, 80% ethanol, 85% ethanol, 90% ethanol, 95% ethanol and absolute ethyl alcohol to carry out serial dehydration to high concentration to the yellow abdomen titmouse sample after fixing process by low concentration, add dehydration gradient, and reduce gradient difference, make dehydration dynamics soft and thorough, sample distortion or sex change in dehydration can be prevented; The present invention adopts sodium hydroxide and dimethylbenzene in conjunction with degreasing, and degreasing effect is better more thorough, and degreasing time is short, decreases preparation of specimen's time to a certain extent, improves make efficiency; The method that the present invention adopts potassium hydroxide and glycerine two kinds of clarifiers to be combined, first use the potassium hydroxide solution of low concentration transparent 2 days, transparent comparatively soft, do not damage sample, to transfer in glycerine potassium hydroxide transparent liquid transparent processing afterwards 5 days, due to the existence of glycerine, reduce the corrosion of potassium hydroxide to sample, the concentration of potassium hydroxide can bring up to more than 3.5%, improves transparent effect; Present invention uses A Li Xinlan dye liquor, make cartilage be colored as blueness, pattern of glassy carcass color is beautiful, more attractive in appearance.
Embodiment
Below in conjunction with specific embodiment, the present invention is described in further detail.
Embodiment 1
A kind of yellow abdomen titmouse pattern of glassy carcass preparation method, comprises the following steps:
Step one, get dead yellow abdomen titmouse process after with 4% formalin fix 10 days;
Step 2,50% ethanol, 70% ethanol, 80% ethanol, 85% ethanol, 90% ethanol, 95% ethanol and absolute ethyl alcohol is adopted to carry out serial dehydration, each concentration gradient 7 day to high concentration to the yellow abdomen titmouse after the fixing process of step one by low concentration;
Step 3, by the yellow abdomen titmouse after dehydration in mass concentration be 1% sodium hydroxide solution in degreasing 3-4 days, then use dimethylbenzene degreasing 1 day;
Step 4, the yellow abdomen titmouse of step 3 degreasing put into the potassium hydroxide solution transparent processing 2 days that mass concentration is 1%, to transfer in glycerine potassium hydroxide transparent liquid transparent processing afterwards 5 days, in described glycerine potassium hydroxide transparent liquid, the mass concentration of potassium hydroxide is 4.5%, mass percent shared by glycerine is 40%, and all the other are water;
Step 5, with Alizarin red staining liquid and A Li Xinlan dyeing liquor, the yellow abdomen titmouse after transparent processing to be dyeed successively, each dyeing 7 days;
Step 6, with 1% potassium hydroxide solution to dyeing after yellow abdomen titmouse sample decolour 5 days, make muscle be water white transparency, obtain yellow abdomen titmouse pattern of glassy carcass;
Step 7, yellow abdomen titmouse pattern of glassy carcass step 6 obtained dewater with 20% glycerite, 30% glycerite, 40% glycerite, 50% glycerite, 60% glycerite, 70% glycerite and 100% glycerine gradient successively;
Step 8, by step 7 dewater after yellow abdomen titmouse pattern of glassy carcass be kept in glycerine, in glycerine, add appropriate preservative (as 0.6% Sodium Benzoate).
Embodiment 2
A kind of yellow abdomen titmouse pattern of glassy carcass preparation method, comprises the following steps:
Step one, get dead yellow abdomen titmouse process after with 4% formalin fix 10 days, then with the yellow abdomen titmouse that running water attitude is fixed, then bleach 7 days with 1% potassium hydroxide solution;
Step 2,50% ethanol, 70% ethanol, 80% ethanol, 85% ethanol, 90% ethanol, 95% ethanol and absolute ethyl alcohol is adopted to carry out serial dehydration, each concentration gradient 7 day to high concentration to the yellow abdomen titmouse after the fixing process of step one by low concentration;
Step 3, by the yellow abdomen titmouse after dehydration in mass concentration be 1% sodium hydroxide solution in degreasing 3-4 days, then use dimethylbenzene degreasing 1 day;
Step 4, the yellow abdomen titmouse of step 3 degreasing put into the potassium hydroxide solution transparent processing 2 days that mass concentration is 1%, to transfer in glycerine potassium hydroxide transparent liquid transparent processing afterwards 5 days, in described glycerine potassium hydroxide transparent liquid, the mass concentration of potassium hydroxide is 4.5%, mass percent shared by glycerine is 40%, and all the other are water;
Step 5, with Alizarin red staining liquid and A Li Xinlan dyeing liquor, the yellow abdomen titmouse after transparent processing to be dyeed successively, each dyeing 7 days;
Step 6, with 20% ammonium glycerin decoloring agent to dyeing after yellow abdomen titmouse sample decolour 5 days, make muscle be water white transparency, obtain yellow abdomen titmouse pattern of glassy carcass;
Step 7, the yellow abdomen titmouse pattern of glassy carcass that step 6 obtained are successively with 20% glycerite, 30% glycerite, 40% glycerite, 50% glycerite, 60% glycerite, 70% glycerite and 100% glycerine gradient dehydration deamination;
Step 8, by step 7 dewater after yellow abdomen titmouse pattern of glassy carcass be kept in glycerine, in glycerine, add appropriate preservative (as 0.6% Sodium Benzoate).
Embodiment 3
A kind of yellow abdomen titmouse pattern of glassy carcass preparation method, comprises the following steps:
Step one, get dead yellow abdomen titmouse process after fix 7 days with absolute ethyl alcohol, then with the yellow abdomen titmouse that running water attitude is fixing, then use 5% hydrogen peroxide bleaching 7 days;
Step 2,50% ethanol, 70% ethanol, 80% ethanol, 85% ethanol, 90% ethanol, 95% ethanol and absolute ethyl alcohol is adopted to carry out serial dehydration, each concentration gradient 7 day to high concentration to the yellow abdomen titmouse after the fixing process of step one by low concentration;
Step 3, by the yellow abdomen titmouse after dehydration in mass concentration be 1% sodium hydroxide solution in degreasing 3-4 days, then use dimethylbenzene degreasing 1 day;
Step 4, the yellow abdomen titmouse of step 3 degreasing put into the potassium hydroxide solution transparent processing 2 days that mass concentration is 1%, to transfer in glycerine potassium hydroxide transparent liquid transparent processing afterwards 5 days, in described glycerine potassium hydroxide transparent liquid, the mass concentration of potassium hydroxide is 4.5%, mass percent shared by glycerine is 40%, and all the other are water;
Step 5, with Alizarin red staining liquid and A Li Xinlan dyeing liquor, the yellow abdomen titmouse after transparent processing to be dyeed successively, each dyeing 7 days;
Step 6, with 1% potassium hydroxide solution to dyeing after yellow abdomen titmouse sample decolour 5 days, make muscle be water white transparency, obtain yellow abdomen titmouse pattern of glassy carcass;
Step 7, yellow abdomen titmouse pattern of glassy carcass step 6 obtained dewater with 20% glycerite, 30% glycerite, 40% glycerite, 50% glycerite, 60% glycerite, 70% glycerite and 100% glycerine gradient successively;
Step 8, by step 7 dewater after yellow abdomen titmouse pattern of glassy carcass be kept in glycerine, in glycerine, add appropriate preservative (as 0.6% Sodium Benzoate).
Embodiment 4
Step one, get dead yellow abdomen titmouse process after fix 7 days with absolute ethyl alcohol, then with the yellow abdomen titmouse that running water attitude is fixing, then bleach 7 days with 1% potassium hydroxide solution;
Step 2,50% ethanol, 70% ethanol, 80% ethanol, 85% ethanol, 90% ethanol, 95% ethanol and absolute ethyl alcohol is adopted to carry out serial dehydration, each concentration gradient 7 day to high concentration to the yellow abdomen titmouse after the fixing process of step one by low concentration;
Step 3, by the yellow abdomen titmouse after dehydration in mass concentration be 1% sodium hydroxide solution in degreasing 3-4 days, then use dimethylbenzene degreasing 1 day;
Step 4, the yellow abdomen titmouse of step 3 degreasing put into the potassium hydroxide solution transparent processing 2 days that mass concentration is 1%, to transfer in glycerine potassium hydroxide transparent liquid transparent processing afterwards 5 days, in described glycerine potassium hydroxide transparent liquid, the mass concentration of potassium hydroxide is 4.5%, mass percent shared by glycerine is 40%, and all the other are water;
Step 5, with Alizarin red staining liquid and A Li Xinlan dyeing liquor, the yellow abdomen titmouse after transparent processing to be dyeed successively, each dyeing 7 days;
Step 6, with 20% ammonium glycerin decoloring agent to dyeing after yellow abdomen titmouse sample decolour 5 days, make muscle be water white transparency, obtain yellow abdomen titmouse pattern of glassy carcass;
Step 7, the yellow abdomen titmouse pattern of glassy carcass that step 6 obtained are successively with 20% glycerite, 30% glycerite, 40% glycerite, 50% glycerite, 60% glycerite, 70% glycerite and 100% glycerine gradient dehydration deamination;
Step 8, by step 7 dewater after yellow abdomen titmouse pattern of glassy carcass be kept in glycerine, in glycerine, add appropriate preservative (as 0.6% Sodium Benzoate).
Embodiment 5
A kind of yellow abdomen titmouse pattern of glassy carcass preparation method, comprises the following steps:
Step one, get dead yellow abdomen titmouse process after with 4% formalin fix 10 days, then with the yellow abdomen titmouse that running water attitude is fixed, then use 5% hydrogen peroxide bleaching 7 days;
Step 2,50% ethanol, 70% ethanol, 80% ethanol, 85% ethanol, 90% ethanol, 95% ethanol and absolute ethyl alcohol is adopted to carry out serial dehydration, each concentration gradient 7 day to high concentration to the yellow abdomen titmouse after the fixing process of step one by low concentration;
Step 3, by the yellow abdomen titmouse after dehydration in mass concentration be 1% sodium hydroxide solution in degreasing 3-4 days, then use dimethylbenzene degreasing 1 day;
Step 4, the yellow abdomen titmouse of step 3 degreasing put into the potassium hydroxide solution transparent processing 2 days that mass concentration is 1%, to transfer in glycerine potassium hydroxide transparent liquid transparent processing afterwards 5 days, in described glycerine potassium hydroxide transparent liquid, the mass concentration of potassium hydroxide is 4.5%, mass percent shared by glycerine is 40%, and all the other are water;
Step 5, with Alizarin red staining liquid and A Li Xinlan dyeing liquor, the yellow abdomen titmouse after transparent processing to be dyeed successively, each dyeing 7 days;
Step 6, with 1% potassium hydroxide solution to dyeing after yellow abdomen titmouse sample decolour 5 days, make muscle be water white transparency, obtain yellow abdomen titmouse pattern of glassy carcass;
Step 7, yellow abdomen titmouse pattern of glassy carcass step 6 obtained dewater with 20% glycerite, 30% glycerite, 40% glycerite, 50% glycerite, 60% glycerite, 70% glycerite and 100% glycerine gradient successively;
Step 8, by step 7 dewater after yellow abdomen titmouse pattern of glassy carcass be kept in glycerine, in glycerine, add appropriate preservative (as 0.6% Sodium Benzoate).
Claims (4)
1. a yellow abdomen titmouse pattern of glassy carcass preparation method, is characterized in that, comprise the following steps:
Step one, get dead yellow abdomen titmouse process after with 4% formalin fix 10 days or absolute ethyl alcohol fixes 7 days;
Step 2,50% ethanol, 70% ethanol, 80% ethanol, 85% ethanol, 90% ethanol, 95% ethanol and absolute ethyl alcohol is adopted to carry out serial dehydration, each concentration gradient 7 day to high concentration to the yellow abdomen titmouse after the fixing process of step one by low concentration;
Step 3, by the yellow abdomen titmouse after dehydration in mass concentration be 1% sodium hydroxide solution in degreasing 3-4 days, then use dimethylbenzene degreasing 1 day;
Step 4, the yellow abdomen titmouse of step 3 degreasing put into the potassium hydroxide solution transparent processing 2 days that mass concentration is 1%, to transfer in glycerine potassium hydroxide transparent liquid transparent processing afterwards 5 days, in described glycerine potassium hydroxide transparent liquid, the mass concentration of potassium hydroxide is 4.5%, mass percent shared by glycerine is 40%, and all the other are water;
Step 5, with Alizarin red staining liquid and A Li Xinlan dyeing liquor, the yellow abdomen titmouse after transparent processing to be dyeed successively, each dyeing 7 days;
Step 6, with 1% potassium hydroxide solution or 20% ammonium glycerin decoloring agent, the yellow abdomen titmouse sample after dyeing to be decoloured 5 days, make muscle be water white transparency, obtain yellow abdomen titmouse pattern of glassy carcass.
2. one according to claim 1 yellow abdomen titmouse pattern of glassy carcass preparation method, is characterized in that: with the yellow abdomen titmouse that running water attitude is fixing before step 2 dehydration, then bleach 7 days with 5% hydrogenperoxide steam generator or 1% potassium hydroxide solution.
3. one according to claim 1 yellow abdomen titmouse pattern of glassy carcass preparation method, is characterized in that: Alizarin red staining liquid described in step 5 is the alizarin red dye liquor of the alizarin red ethanol dye liquor of 2% or 0.04% containing 2% potassium hydroxide; Described A Li Xinlan dyeing liquor is formed by the proportions containing 70% ethanolic solution of 0.2% A Li Xinlan, 70% ethanolic solution and glacial acetic acid 1:18:1 by volume.
4. one according to claim 1 yellow abdomen titmouse pattern of glassy carcass preparation method, is characterized in that: yellow abdomen titmouse pattern of glassy carcass step 6 obtained dewaters with 20% glycerite, 30% glycerite, 40% glycerite, 50% glycerite, 60% glycerite, 70% glycerite and 100% glycerine gradient successively.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110278939A (en) * | 2019-07-03 | 2019-09-27 | 秦家哲 | A kind of production method and its application of transparent stained preparation |
| CN110720452A (en) * | 2019-11-05 | 2020-01-24 | 南通大学 | Method for optimizing preservation of pathological gross specimens |
| CN114982742A (en) * | 2022-05-23 | 2022-09-02 | 内蒙古农业大学 | Method for preparing transparent sample of embryonic skeleton of 4-21 days old chicken |
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| CN104585160A (en) * | 2014-12-31 | 2015-05-06 | 上海师范大学 | Novel method for preparing transparent skeleton specimen and storing resin |
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104585160A (en) * | 2014-12-31 | 2015-05-06 | 上海师范大学 | Novel method for preparing transparent skeleton specimen and storing resin |
Non-Patent Citations (2)
| Title |
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| 吕建伟等: "透明骨骼标本制作", 《高师理科学刊》 * |
| 李锦: "小白鼠透明骨骼标本制作技术的改进", 《大连教育学院学报》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110278939A (en) * | 2019-07-03 | 2019-09-27 | 秦家哲 | A kind of production method and its application of transparent stained preparation |
| CN110720452A (en) * | 2019-11-05 | 2020-01-24 | 南通大学 | Method for optimizing preservation of pathological gross specimens |
| CN114982742A (en) * | 2022-05-23 | 2022-09-02 | 内蒙古农业大学 | Method for preparing transparent sample of embryonic skeleton of 4-21 days old chicken |
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Application publication date: 20151216 |