CN104585160B - Novel transparent skeleton specimen makes and resin store method - Google Patents
Novel transparent skeleton specimen makes and resin store method Download PDFInfo
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- CN104585160B CN104585160B CN201410849529.0A CN201410849529A CN104585160B CN 104585160 B CN104585160 B CN 104585160B CN 201410849529 A CN201410849529 A CN 201410849529A CN 104585160 B CN104585160 B CN 104585160B
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Abstract
The invention discloses a kind of novel transparent skeleton specimen to make and resin store method.By pretreatment, dehydration, bleaching, transparent, dyeing, decolouring, again glycerol transparent, de-, dry and the step such as seal up for safekeeping, the invention solves that current store method can cause specimen to fade, mouldy, transparency declines, and transport inconvenience, the problems such as solution need to be regularly replaced.Instant invention overcomes all difficult problems during existing pattern of glassy carcass makes and preserves, the method has the advantage that fabrication cycle is short, and made specimen easily puts on display preservation, and transparency is high, undergoes no deterioration mouldy, the advantage that can preserve for a long time.
Description
Technical field
The invention belongs to biology techniques field, relate to new type resin pattern of glassy carcass manufacturing technology.
Background technology
The physiological important content of animal movement is not only in vertebrate skeletal research, is also the important evidence of animal classification.Pattern of glassy carcass, can be shown that and be difficult to now examine clearly structure with general anatomic method.It can demonstrate internal bone portion when keeping specimen profile complete, it is shown that the skeleton development situation of organism.Its producing principle is that chemical drugs and physical method are acted on when injected organism tissue, and the index of refraction making the index of refraction of tissue and clarifier is close, to reach transparent purpose.The specimen prepared is vivid, it is easy to skeleton is carried out home position observation, and third dimension is stronger, and can show skeletal system NATURAL DISTRIBUTION in vivo and position on the whole, manifests the development condition of animal body endoskeleton system.Simultaneously as its magnificent beautiful colour forming, and there is artistic value.
But, current pattern of glassy carcass fabrication cycle length and owing at present only there is a kind of liquid glycerin store method, the reason that the method preserves, specimen can be caused to fade, mouldy, transparency declines, transport inconvenience, need to regularly replacing solution, these follow-up problems greatly suppress pattern of glassy carcass development in bioconversion medium, museum's collection, activity to popularize scientific knowledge.
Summary of the invention
The present invention provides the manufacture method of a kind of new solid-state specimen.
Present invention employs following technical scheme:
A kind of novel transparent skeleton specimen makes and resin store method, it is characterized in that: include step:
(1) spoil within collecting dead 1 day, removes epidermis, and opens osculum from cloaca upper end, remove all of internal organs, and large mammal removes eyeball, and Fish remove the cheek, scale；
(2) fixed preparation, is dipped in volumetric concentration 95% ethanol, dehydration is taken off for 1-2 days to its epidermal pigment by the specimen of collection；
(3) melanin therein within 1-2 hour, is got rid of with the hydrogen peroxide bleaching of mass concentration 3%；
(4) with mass concentration 3% potassium hydroxide soak 2-5 days transparent to it；
(5) using dyeing liquor soaking and dyeing one to two days, once, dyeing liquor quality proportioning is potassium hydroxide: alizarin red: water=1:0.01:100 in stirring in every 6 hours；
(6) solar exposure two days, change a destaining solution decolouring for one day, and destaining solution volume proportion is glycerol: water=1:1；
(7) soak one day with glycerol, within omnidistance 24 hours, irradiate with tengsten lamp, glycerol wherein adds the thymol of its quality 0.01%；
(8) with alcohol-pickled 2 hours de-glycerol of volumetric concentration 75%；
(9) dry, silica-gel desiccant embeds 6 hours；
(10) seal up for safekeeping, choose proper mold, epoxy resin is poured into one layer of entrance, put into specimen second time after one hour and pour epoxy resin into and embed, if specimen floating, then in Surface mulch third layer after six hours.
Instant invention overcomes all difficult problems during existing pattern of glassy carcass makes and preserves, it is short that the method has fabrication cycle, it is easy to puts on display and preserves, and transparency is high, undergoes no deterioration mouldy, the advantage that can preserve for a long time.
Detailed description of the invention
Embodiment:
(1) pretreatment: collect fresh spoil, namely within dead 1 day, corpse removes epidermis, and opens osculum from cloaca upper end, removes all of internal organs, and large mammal removes eyeball, and Fish to remove the cheek, scale.
(2) dehydration: fixed preparation, is dipped in volumetric concentration 95% ethanol dehydration 1-5 days by the specimen of collection, and dehydration natural law depends on the size of animal, if Fish, 1-2 days；Large mammal 3-5 days, till taking off depending on its epidermal pigment.
(3) bleaching: get rid of melanin therein, with hydrogen peroxide bleaching 1-2 hour of quality lane 3%.
(4) transparent: to soak 2-5 days with mass concentration 3% potassium hydroxide, determine depending on its size, if the animal that build is bigger, available 5% potassium hydroxide solution is injected at muscle thicker part, it is possible to accelerate the transparent speed of specimen.
(5) dyeing: dyeing liquor quality proportioning is potassium hydroxide: alizarin red: water=1:0.01:100 mono-to two days, stirring in every 6 hours is once, it is prevented that dye liquor forms complex compound sediment and causes dyeing inequality.
(6) decolouring: destaining solution volume proportion is glycerol: water=1:1, solar exposure, two days, changes a destaining solution in one day, and solar exposure is conducive to the pigmentolysis in muscle.
(7) transparent again: quality is than glycerol: thymol=100:0.01 mono-day, within 24 hours, tengsten lamp irradiates.It is mouldy that thymol desirably prevents specimen, and tengsten lamp is conducive to accelerating the transparent of muscular tissue, and this improves and the transparent speed of specimen can improve 3～5 times.
(8) de-glycerol: alcohol-pickled 2 hours of volumetric concentration 75%, removes the glycerol on top layer.It is affect transparency to solve directly can permeate by resin embedding specimen to be dissolved in resin to cause bleaching that glycerol is fallen in elimination, it is possible to be greatly promoted solid specimen transparency.
(9) dry: silica-gel desiccant embeds 6 hours, is completely dried top layer moisture, it is prevented that specimen is mouldy and affects transparency.
(10) seal up for safekeeping: choose proper mold, epoxy resin is poured into one layer of entrance, put into specimen second time after one hour and pour epoxy resin into and embed, if specimen floating, then in Surface mulch third layer after six hours.Because pattern of glassy carcass is floating through the relatively low meeting of dried density, therefore need point three pouring embeddings.
Claims (2)
1. novel transparent skeleton specimen makes and a resin store method, it is characterized in that: include step:
(1) spoil within collecting dead 1 day, removes epidermis, and opens osculum from cloaca upper end, remove all of internal organs, and large mammal removes eyeball, and Fish remove the cheek, scale；
(2) fixed preparation, is dipped in volumetric concentration 95% ethanol, dehydration is taken off for 1-2 days to its epidermal pigment by the specimen of collection；
(3) melanin therein within 1-2 hour, is got rid of with the hydrogen peroxide bleaching of mass concentration 3%；
(4) with mass concentration 3% potassium hydroxide soak 2-5 days transparent to it；
(5) using dyeing liquor soaking and dyeing one to two days, once, dyeing liquor quality proportioning is potassium hydroxide: alizarin red: water=1:0.01:100 in stirring in every 6 hours；
(6) solar exposure two days, change a destaining solution decolouring for one day, and destaining solution volume proportion is glycerol: water=1:1；
(7) soak one day with glycerol, within omnidistance 24 hours, irradiate with tengsten lamp, glycerol wherein adds the thymol of its quality 0.01%；
(8) with alcohol-pickled 2 hours de-glycerol of volumetric concentration 75%；
(9) dry, silica-gel desiccant embeds 6 hours；
(10) seal up for safekeeping, choose proper mold, epoxy resin is poured into one layer of entrance, put into specimen second time after 1 hour and pour epoxy resin into and embed, if specimen floating, then after 6 hours in Surface mulch third layer.
2. method according to claim 1, it is characterised in that if the bigger animal of build in step (4), available 5% potassium hydroxide solution is injected at muscle thicker part.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410849529.0A CN104585160B (en) | 2014-12-31 | 2014-12-31 | Novel transparent skeleton specimen makes and resin store method |
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| CN201410849529.0A CN104585160B (en) | 2014-12-31 | 2014-12-31 | Novel transparent skeleton specimen makes and resin store method |
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| CN104585160A CN104585160A (en) | 2015-05-06 |
| CN104585160B true CN104585160B (en) | 2016-07-06 |
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Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104872110A (en) * | 2015-05-28 | 2015-09-02 | 暨南大学 | Crystal skeleton specimen manufacturing method |
| CN105145544A (en) * | 2015-09-30 | 2015-12-16 | 河南科技大学 | Method for making transparent parusvenustulus skeleton specimen |
| CN105973663B (en) * | 2016-04-29 | 2019-07-12 | 马征来 | A kind of preparation method of colorful skeleton specimen |
| CN107183002A (en) * | 2017-05-10 | 2017-09-22 | 广西柳州中嘉知识产权服务有限公司 | Butterfly specimen preparation method |
| CN107279130A (en) * | 2017-06-16 | 2017-10-24 | 大连海洋大学 | The double dyeing plastic packaging Slide processings of fish bone |
| CN107258764A (en) * | 2017-06-16 | 2017-10-20 | 大连海洋大学 | Cartilaginous fish skeleton specimen preparation method |
| CN107306935A (en) * | 2017-06-20 | 2017-11-03 | 大连海洋大学 | Bony fish bone stain plastic packaging Slide processing |
| CN110278939A (en) * | 2019-07-03 | 2019-09-27 | 秦家哲 | A kind of production method and its application of transparent stained preparation |
| CN110720452B (en) * | 2019-11-05 | 2021-11-19 | 南通大学 | Method for optimizing preservation of pathological gross specimens |
| CN112825845A (en) * | 2019-11-25 | 2021-05-25 | 石功鹏阳 | Specimen display method |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1480038A (en) * | 2003-07-28 | 2004-03-10 | 王孔宝 | Embedding material biological sample and method for making specimen of human body |
| WO2008073915A3 (en) * | 2006-12-08 | 2008-10-23 | Asuragen Inc | Micrornas differentially expressed in leukemia and uses thereof |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1480038A (en) * | 2003-07-28 | 2004-03-10 | 王孔宝 | Embedding material biological sample and method for making specimen of human body |
| WO2008073915A3 (en) * | 2006-12-08 | 2008-10-23 | Asuragen Inc | Micrornas differentially expressed in leukemia and uses thereof |
Non-Patent Citations (1)
| Title |
|---|
| 滕斌.透明骨骼标本的速成制作方法.《生物学杂志》.1987,(第15期), * |
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