CN107279130A - The double dyeing plastic packaging Slide processings of fish bone - Google Patents
The double dyeing plastic packaging Slide processings of fish bone Download PDFInfo
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- CN107279130A CN107279130A CN201710455892.8A CN201710455892A CN107279130A CN 107279130 A CN107279130 A CN 107279130A CN 201710455892 A CN201710455892 A CN 201710455892A CN 107279130 A CN107279130 A CN 107279130A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
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- G—PHYSICS
- G09—EDUCATION; CRYPTOGRAPHY; DISPLAY; ADVERTISING; SEALS
- G09B—EDUCATIONAL OR DEMONSTRATION APPLIANCES; APPLIANCES FOR TEACHING, OR COMMUNICATING WITH, THE BLIND, DEAF OR MUTE; MODELS; PLANETARIA; GLOBES; MAPS; DIAGRAMS
- G09B23/00—Models for scientific, medical, or mathematical purposes, e.g. full-sized devices for demonstration purposes
- G09B23/36—Models for scientific, medical, or mathematical purposes, e.g. full-sized devices for demonstration purposes for zoology
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Abstract
The present invention discloses a kind of double dyeing plastic packaging Slide processings of fish bone, and its condition and step are：By the fish body internal organ of the fish with two kinds of bones of os osseum and cartilage, scale and skin are cleaned out, distilled water immersion is used after being fixed through 10% formalin again 12 days, ensure that formalin is removed clean, it is blueness that dyestuff alcian blue, which is reused, bone and cartilage dye, then presentation pellucidity is made using trypsin treatment fish body muscle after the ethanol postincubation of various concentrations, while the blueness of cartilage-preserving, os osseum is dyed into red with alizarin red again, fish body is plastified using polyvinylpyrrolidone after 0.5%KOH and glycerine mixed solution through different proportion are transparent once again, sample is air-dried after the completion of plasticizing, finally sample is sealed up for safekeeping with epoxy resin.After the completion of sample can directly throw resin and plasticizing muscle see os osseum dye red cartilage dye blueness skeleton specimen.
Description
Technical field
The present invention relates to a kind of fish skeleton specimen preparation method, the double dyeing plastic packaging sample systems of particularly a kind of fish bone
Make method.
Background technology
Fish skeleton specimen is a part for ichthyology teaching, is also the important composition of Popular Science Education.Pass through the bone of fish
Bone sample can help the knowledge of the study fish skeletal system of student and children image.Traditional fish bone preparation method has
Two kinds：One is to reject the whole muscle parts of fish, bone is reassembled into original state, manufacturing process is cumbersome, easily
The tiny bone of lost part or the assembled mistake of bone site, the producing efficiency of skeleton specimen are relatively low, incomplete fish bone
Bone sample also reduces the meaning of education.Two be after a series of transparent and dyeing course, by Saving specimen in glycerine,
But the glycerine of liquid is not easy to storage and transport.The manufacturing technology of current fish skeleton specimen is significantly improved space.
The content of the invention
The present invention is that a kind of fish bone of proposition is double to dye plastic packaging marks in order to solve the above-mentioned deficiency present in prior art
This preparation method.
The present invention technical solution be：A kind of double dyeing plastic packaging Slide processings of fish bone, it is characterised in that：
Carry out successively fish body preparation, cartilage dyeing, osmotic pressure adjustment, the enzyme solutions immersion of fish body, os osseum dyeing, secondary transparent processing,
Sample is plastified and sample encapsulation step, final to obtain the double dyeing plastic packaging samples of fish bone.
Described fish body preparation process is：The fish of fresh, the complete fish with two kinds of bones of os osseum and cartilage of selection
Body, fish body is removed the internal organs, after scale and fish-skin, is immersed in 10% formalin fixer 2 days, can also protected for a long time
Deposit, be then immersed in fish body in distilled water 1-2 days, remove the formalin in fish body, it is stand-by.
Described cartilage staining procedure is：The fish body treated by fish body preparation process is put into mixing dye liquor and carried out
Dyeing 2-3 days, described mixing dye liquor is mixed by alcian blue, concentration for 95% ethanol and glacial acetic acid, and alcian blue
Mass volume ratio with glacial acetic acid is 1:200g/ml, and ethanol with and glacial acetic acid volume ratio be 80:20-75:25.
Described osmotic pressure set-up procedure is：It is 95% that the fish body treated by cartilage staining procedure is transferred into concentration
Alcohol in, after 1-2 hours change an alcohol, then by fish body successively respectively concentration for 70%, 40% and 15% alcohol soak
Bubble, each concentration gradient soak time is 2-3 hours, is finally put into fish body in distilled water 2-3 hours, is sunk to sample
To distilled water bottom.
Described enzymolysis liquid soaking step is：The fish body treated by osmotic pressure set-up procedure is immersed in enzyme solutions
In, an enzyme solutions were changed per 2-3 days, or enzyme solutions are changed when solution becomes au bleu, until the os osseum and cartilage of fish body
High-visible, the blueness on muscle is taken off, and described enzyme solutions are trypsase, the dobell's solution of saturation and distilled water
Mixture, wherein trypsase, the mass volume ratio of saturation dobell's solution are 1：30g/ml-2：30g/ml, saturation Boratex
The volume ratio of solution and distilled water is 3.0:7.0-3.5:6.5.
Described os osseum staining procedure is：Fish body after enzyme solutions immersion treatment is moved into alizarin red dye liquor and carried out
Dyeing, soak time is 24 hours, until fish body bone dyes red, described alizarin red dye liquor is that alizarin red is with concentration
The mass volume ratio of the mixture of 0.5% KOH solution, wherein alizarin red and KOH solution is 0.05:100g/ml.
Described secondary transparent processing step is：Fish body after the processing of os osseum staining procedure is respectively placed in first saturating
Soaked in bright processing solution and the second transparent processing solution, soak time is 3-4 days, and the first described transparent processing solution is
Glycerine and concentration are the mixture of 0.5% KOH solution, and the volume ratio of glycerine and KOH solution is 1:3, the second transparent place
The mixture for the KOH solution that reason solution is equally glycerine and concentration is 0.5%, and the volume ratio of glycerine and KOH solution is 1:1.
Described sample plasticizing process is：Using concentration be 0.5% KOH solution as mother liquor, concentration is respectively configured for 25% He
50% polyvinylpyrrolidonesolution solution, concentration is that polyvinylpyrrolidone in 25% polyvinylpyrrolidonesolution solution and KOH are molten
The mass volume ratio of liquid is 25：100g/ml, concentration is polyvinylpyrrolidone and KOH in 50% polyvinylpyrrolidonesolution solution
The mass volume ratio of solution is 50：100g/ml, by the fish body sample Jing Guo secondary transparent processing successively concentration for 25% it is poly-
With concentration to be soaked in 50% polyvinylpyrrolidonesolution solution in vinylpyrrolidone solution, each soak time is
5-6 days, after immersion terminates, fish body sample is subjected to natural air drying 9-11 days, or the constant temperature drying 30-40 under conditions of 25 DEG C
Hour.
Described sample encapsulation step is：By epoxy resin A, B glue according to 2.5:1 ratio is well mixed, will be by mark
Fish-bone sample after this plastics processing is immersed in mixed epoxy resin, and 24-30 hours are stood under normal temperature, and epoxy resin coagulates
Gu, after polishing, the profile of finishing sample, obtain the double dyeing plastic packaging samples of fish bone.
The present invention compared with the existing technology, has the following advantages that：
The double dyeing plastic packaging Slide processings of fish bone disclosed in this invention, result in complete pattern of glassy carcass,
And because the refractive index of the muscle after enzymolysis processing, the glycerine in fish body and epoxy resin three is sufficiently close to, therefore
The bone of dyeing can be observed directly through resin and muscle, allows final sample that a kind of " amber " state is presented.
This method need not disassemble the skeletal system of fish body, and the integrality of skeletal system can be kept to greatest extent,
As the epoxy resin of capsulation material it is simultaneously a kind of harmless material, uses safety ' and the sample of amber state
Student and children's study interest can also be increased, or even this sample can also be shown as handicraft, and it
Also there is the characteristic of convenient transport.It can be said that this method possesses a variety of advantages, it is particularly suitable for promoting in the art
Using its market prospects is very wide.
Embodiment
The embodiment of the present invention is described below.
A kind of double dyeing plastic packaging Slide processings of fish bone, its condition and step are：Carry out fish body preparation successively, it is soft
Bone dyeing, osmotic pressure adjustment, the enzyme solutions immersion of fish body, os osseum dyeing, the plasticizing of secondary transparent processing, sample and sample encapsulation step
Suddenly, it is final to obtain the double dyeing plastic packaging samples of fish bone.
Fish body prepares：The fresh complete fish with two kinds of bones of os osseum and cartilage of selection remove as sample material
It is immersed in after internal organ, scale and fish-skin in 10% formalin fixer, 2-3 days or long-term preservation, if sample exists
In formalin or alcohol, it can be immersed directly in distilled water.Fish body is immersed in distilled water 1-2 days, is removed formalin, is treated
With.
The fish body treated by fish body preparation process is put into mixing dye liquor and carries out dyeing 2-3 days, described mixing
Dye liquor is that 95% ethanol and 20-25ml glacial acetic acid are mixed by 0.1g alcian blue, 75-80ml concentration, mixing dye
Liquid matching while using.
The fish body treated by cartilage staining procedure is transferred in the alcohol that concentration is 95%, one is changed after 1-2 hours
Secondary alcohol, then by fish body successively respectively concentration for 70%, 40% and 15% it is alcohol-pickled, each concentration gradient soak time is equal
For 2-3 hours, finally fish body is put into distilled water 2-3 hours, distilled water bottom is sunk down into sample
The fish body treated by osmotic pressure set-up procedure is immersed in enzyme solutions, an enzyme solutions were changed per 2-3 days, or
Enzyme solutions are changed when solution becomes au bleu, until the os osseum and cartilage of fish body are high-visible, the blueness on muscle is taken off, institute
The enzyme solutions stated are the mixture of trypsase, the dobell's solution of saturation and distilled water, and wherein trypsase is 1-2g, saturation
Dobell's solution is 30-35ml, and distilled water is 65-70ml.
Fish body after enzyme solutions immersion treatment is moved into and dyed in alizarin red dye liquor, soak time is 24 small
When, until fish body bone dyes red, described alizarin red dye liquor is the mixing for the KOH solution that alizarin red is 0.5% with concentration
Thing, wherein alizarin red are 0.05g, and KOH solution is 100ml.
Fish body after the processing of os osseum staining procedure is respectively placed in the first transparent processing solution and the second transparent processing
Soaked in solution, soak time is 3-4 days, and the first described KOH that transparent processing solution is glycerine and concentration is 0.5% is molten
The mixture of liquid, and the volume ratio of glycerine and KOH solution is 1:3, the second transparent processing solution is equally glycerine and concentration
It is 1 for the volume ratio of the mixture of 0.5% KOH solution, and glycerine and KOH solution:1.
Using concentration be 0.5% KOH solution as mother liquor, be respectively configured concentration be 25% and 50% polyvinylpyrrolidone it is molten
Polyvinylpyrrolidone and the mass volume ratio of KOH solution are 25 in liquid, the polyvinylpyrrolidonesolution solution that concentration is 25%：
Polyvinylpyrrolidone and the mass volume ratio of KOH solution are in 100g/ml, the polyvinylpyrrolidonesolution solution that concentration is 50%
50：100g/ml, by the fish body sample Jing Guo secondary transparent processing successively in concentration in 25% polyvinylpyrrolidonesolution solution
With concentration to be soaked in 50% polyvinylpyrrolidonesolution solution, each soak time is 5-6 days, after immersion terminates, will
Fish body sample carries out natural air drying 9-11 days, or constant temperature drying 30-40 hours under conditions of 25 DEG C.
By epoxy resin A, B glue according to 2.5:1 ratio is well mixed, by the fish-bone sample after sample plastics processing
It is immersed in mixed epoxy resin, 24-30 hours is stood under normal temperature, epoxy resin solidification, polishing, the profile of finishing sample
Afterwards, bony fish bone stain plastic packaging sample is obtained.
Claims (9)
1. a kind of double dyeing plastic packaging Slide processings of fish bone, it is characterised in that：Fish body preparation, cartilage dye are carried out successively
Color, osmotic pressure adjustment, the enzyme solutions immersion of fish body, os osseum dyeing, the plasticizing of secondary transparent processing, sample and sample encapsulation step,
It is final to obtain the double dyeing plastic packaging samples of fish bone.
2. the double dyeing plastic packaging Slide processings of fish bone according to claim 1, it is characterised in that：Described fish body
Preparation process is：The fish body of fresh, the complete fish with two kinds of bones of os osseum and cartilage of selection, fish body is removed the internal organs,
After scale and fish-skin, it is immersed in 10% formalin fixer 2 days, also can for a long time preserves, then be immersed in fish body
1-2 days in distilled water, the formalin in fish body is removed, it is stand-by.
3. the double dyeing plastic packaging Slide processings of fish bone according to claim 1, it is characterised in that：
Described cartilage staining procedure is：The fish body treated by fish body preparation process is put into mixing dye liquor and dyed
2-3 days, described mixing dye liquor was mixed by alcian blue, concentration for 95% ethanol and glacial acetic acid, and alcian blue and ice
The mass volume ratio of acetic acid is 1:200g/ml, and ethanol with and glacial acetic acid volume ratio be 80:20-75:25.
4. the double dyeing plastic packaging Slide processings of fish bone according to claim 1, it is characterised in that：
Described osmotic pressure set-up procedure is：The fish body treated by cartilage staining procedure is transferred to the wine that concentration is 95%
In essence, after 1-2 hours change an alcohol, then by fish body successively respectively concentration for 70%, 40% and 15% it is alcohol-pickled, often
Individual concentration gradient soak time is 2-3 hours, is finally put into distilled water fish body 2-3 hours, steaming is sunk down into sample
Distilled water bottom.
5. the double dyeing plastic packaging Slide processings of fish bone according to claim 1, it is characterised in that：Described enzymolysis
Liquid soaking step is：The fish body treated by osmotic pressure set-up procedure is immersed in enzyme solutions, an enzyme was changed per 2-3 days
Solution, or enzyme solutions are changed when solution becomes au bleu, until the os osseum and cartilage of fish body are high-visible, the indigo plant on muscle
Color takes off, and described enzyme solutions are the mixture of trypsase, the dobell's solution of saturation and distilled water, wherein trypsase,
The mass volume ratio of saturation dobell's solution is 1：30g/ml-2：The volume ratio of 30g/ml, saturation dobell's solution and distilled water
For 3.0:7.0-3.5:6.5.
6. the double dyeing plastic packaging Slide processings of fish bone according to claim 1, it is characterised in that：
Described os osseum staining procedure is：Fish body after enzyme solutions immersion treatment is moved into and contaminated in alizarin red dye liquor
Color, soak time is 24 hours, until fish body bone dyes red, and described alizarin red dye liquor is alizarin red and concentration is 0.5%
KOH solution mixture, wherein the mass volume ratio of alizarin red and KOH solution be 0.05:100g/ml.
7. the double dyeing plastic packaging Slide processings of fish bone according to claim 1, it is characterised in that：
Described secondary transparent processing step is：Fish body after the processing of os osseum staining procedure is respectively placed in the first transparent place
Manage in solution and the second transparent processing solution and soak, soak time is 3-4 days, the first described transparent processing solution is glycerine
Volume ratio with mixture of the concentration for 0.5% KOH solution, and glycerine and KOH solution is 1:3, second transparent processing is molten
The mixture for the KOH solution that liquid is equally glycerine and concentration is 0.5%, and the volume ratio of glycerine and KOH solution is 1:1.
8. the double dyeing plastic packaging Slide processings of fish bone according to claim 1, it is characterised in that：Described sample
Plasticizing process is：Using concentration be 0.5% KOH solution as mother liquor, be respectively configured concentration be 25% and 50% polyvinylpyrrolidone
Polyvinylpyrrolidone and the mass volume ratio of KOH solution are 25 in solution, the polyvinylpyrrolidonesolution solution that concentration is 25%：
Polyvinylpyrrolidone and the mass volume ratio of KOH solution are in 100g/ml, the polyvinylpyrrolidonesolution solution that concentration is 50%
50：100g/ml, by the fish body sample Jing Guo secondary transparent processing successively in concentration in 25% polyvinylpyrrolidonesolution solution
With concentration to be soaked in 50% polyvinylpyrrolidonesolution solution, each soak time is 5-6 days, after immersion terminates, will
Fish body sample carries out natural air drying 9-11 days, or constant temperature drying 30-40 hours under conditions of 25 DEG C.
9. the double dyeing plastic packaging Slide processings of fish bone according to claim 1, it is characterised in that：Described sample
Encapsulation step is：By epoxy resin A, B glue according to 2.5:1 ratio is well mixed, by the fish-bone after sample plastics processing
Sample is immersed in mixed epoxy resin, and 24-30 hours are stood under normal temperature, epoxy resin solidification, is polished, is repaired sample
After profile, the double dyeing plastic packaging samples of fish bone are obtained.
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Cited By (9)
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| CN109272844A (en) * | 2018-10-31 | 2019-01-25 | 海南来者实业有限公司 | A method of it reinforcing fishes snd fin and repairs film between fin simultaneously |
| CN110089516A (en) * | 2019-05-28 | 2019-08-06 | 中国长江三峡集团有限公司中华鲟研究所 | A kind of production method of novel larva and juvenile sample |
| CN110278939A (en) * | 2019-07-03 | 2019-09-27 | 秦家哲 | A kind of production method and its application of transparent stained preparation |
| CN110432257A (en) * | 2019-08-01 | 2019-11-12 | 上海海洋大学 | A kind of crab class dry preserved specimen production method |
| CN111678756A (en) * | 2020-06-12 | 2020-09-18 | 苏州木芮生物科技有限公司 | One-step kit for dyeing zebra fish cartilage |
| CN112042574A (en) * | 2020-10-14 | 2020-12-08 | 苏州木芮生物科技有限公司 | Construction method and application of zebra fish osteoporosis model |
| CN112903400A (en) * | 2021-01-13 | 2021-06-04 | 河南中博生物塑化科技有限公司 | Method for dyeing blood vessels and nerves of human plasticized specimen |
| CN113812393A (en) * | 2021-08-09 | 2021-12-21 | 安徽农业大学 | Method for manufacturing mouse double-dyeing transparent skeleton plastic package embedding specimen |
| CN114982742A (en) * | 2022-05-23 | 2022-09-02 | 内蒙古农业大学 | Method for preparing transparent sample of embryonic skeleton of 4-21 days old chicken |
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109272844A (en) * | 2018-10-31 | 2019-01-25 | 海南来者实业有限公司 | A method of it reinforcing fishes snd fin and repairs film between fin simultaneously |
| CN110089516A (en) * | 2019-05-28 | 2019-08-06 | 中国长江三峡集团有限公司中华鲟研究所 | A kind of production method of novel larva and juvenile sample |
| CN110278939A (en) * | 2019-07-03 | 2019-09-27 | 秦家哲 | A kind of production method and its application of transparent stained preparation |
| CN110432257A (en) * | 2019-08-01 | 2019-11-12 | 上海海洋大学 | A kind of crab class dry preserved specimen production method |
| CN111678756A (en) * | 2020-06-12 | 2020-09-18 | 苏州木芮生物科技有限公司 | One-step kit for dyeing zebra fish cartilage |
| CN112042574A (en) * | 2020-10-14 | 2020-12-08 | 苏州木芮生物科技有限公司 | Construction method and application of zebra fish osteoporosis model |
| CN112903400A (en) * | 2021-01-13 | 2021-06-04 | 河南中博生物塑化科技有限公司 | Method for dyeing blood vessels and nerves of human plasticized specimen |
| CN112903400B (en) * | 2021-01-13 | 2023-03-14 | 河南中博生物塑化科技有限公司 | Method for dyeing blood vessels and nerves of human plasticized specimen |
| CN113812393A (en) * | 2021-08-09 | 2021-12-21 | 安徽农业大学 | Method for manufacturing mouse double-dyeing transparent skeleton plastic package embedding specimen |
| CN114982742A (en) * | 2022-05-23 | 2022-09-02 | 内蒙古农业大学 | Method for preparing transparent sample of embryonic skeleton of 4-21 days old chicken |
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