CN112903400A - Method for dyeing blood vessels and nerves of human plasticized specimen - Google Patents
Method for dyeing blood vessels and nerves of human plasticized specimen Download PDFInfo
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- CN112903400A CN112903400A CN202110041582.8A CN202110041582A CN112903400A CN 112903400 A CN112903400 A CN 112903400A CN 202110041582 A CN202110041582 A CN 202110041582A CN 112903400 A CN112903400 A CN 112903400A
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- 210000004204 blood vessel Anatomy 0.000 title claims abstract description 55
- 210000005036 nerve Anatomy 0.000 title claims abstract description 55
- 238000004043 dyeing Methods 0.000 title claims abstract description 21
- 238000000034 method Methods 0.000 title claims abstract description 16
- 239000000049 pigment Substances 0.000 claims abstract description 25
- CTQNGGLPUBDAKN-UHFFFAOYSA-N O-Xylene Chemical compound CC1=CC=CC=C1C CTQNGGLPUBDAKN-UHFFFAOYSA-N 0.000 claims abstract description 24
- 239000003292 glue Substances 0.000 claims abstract description 19
- 239000002966 varnish Substances 0.000 claims abstract description 18
- 239000003822 epoxy resin Substances 0.000 claims abstract description 15
- 229920000647 polyepoxide Polymers 0.000 claims abstract description 15
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 10
- 150000002978 peroxides Chemical class 0.000 claims abstract description 9
- 239000003086 colorant Substances 0.000 claims abstract description 6
- 238000010790 dilution Methods 0.000 claims abstract description 6
- 239000012895 dilution Substances 0.000 claims abstract description 6
- 238000004061 bleaching Methods 0.000 claims abstract description 5
- 239000011248 coating agent Substances 0.000 claims description 15
- 238000000576 coating method Methods 0.000 claims description 15
- 239000000975 dye Substances 0.000 claims description 12
- 238000010186 staining Methods 0.000 claims description 8
- 238000001035 drying Methods 0.000 claims description 7
- 238000002156 mixing Methods 0.000 claims description 7
- 239000004115 Sodium Silicate Substances 0.000 claims description 5
- 239000001055 blue pigment Substances 0.000 claims description 5
- 238000004140 cleaning Methods 0.000 claims description 5
- 238000005562 fading Methods 0.000 claims description 5
- 238000002360 preparation method Methods 0.000 claims description 5
- 239000001054 red pigment Substances 0.000 claims description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 5
- 230000001590 oxidative effect Effects 0.000 claims description 4
- 239000004972 Polyurethane varnish Substances 0.000 claims description 3
- NIXOWILDQLNWCW-UHFFFAOYSA-N acrylic acid group Chemical group C(C=C)(=O)O NIXOWILDQLNWCW-UHFFFAOYSA-N 0.000 claims description 3
- 229920000180 alkyd Polymers 0.000 claims description 3
- 229920003986 novolac Polymers 0.000 claims description 2
- 238000007711 solidification Methods 0.000 claims description 2
- 230000008023 solidification Effects 0.000 claims description 2
- 239000011259 mixed solution Substances 0.000 abstract 1
- 238000010422 painting Methods 0.000 abstract 1
- 239000000047 product Substances 0.000 description 7
- 239000012466 permeate Substances 0.000 description 6
- 230000007774 longterm Effects 0.000 description 5
- 241001465754 Metazoa Species 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 238000001125 extrusion Methods 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N phenol group Chemical group C1(=CC=CC=C1)O ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 3
- 238000012216 screening Methods 0.000 description 3
- 210000001367 artery Anatomy 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 238000004321 preservation Methods 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 238000007447 staining method Methods 0.000 description 2
- 238000003860 storage Methods 0.000 description 2
- 210000003462 vein Anatomy 0.000 description 2
- 239000008096 xylene Substances 0.000 description 2
- 241000238631 Hexapoda Species 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 231100000956 nontoxicity Toxicity 0.000 description 1
- 238000009896 oxidative bleaching Methods 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 238000002791 soaking Methods 0.000 description 1
- 229910052911 sodium silicate Inorganic materials 0.000 description 1
- 230000009967 tasteless effect Effects 0.000 description 1
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N1/31—Apparatus therefor
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N2001/302—Stain compositions
Abstract
The invention relates to a method for dyeing blood vessels and nerves of a human plasticized specimen, which belongs to the technical field of biological plasticization and comprises the following steps: removing surface glue from a plasticized specimen needing to display blood vessel and nerve structures and walking shape by dimethylbenzene, and bleaching by using peroxide. Then adding ethanol into the mixed solution of the pigment toner and the epoxy resin AB glue for dilution, and respectively painting blood vessels and nerves after adjusting to proper colors; then curing in a vacuum pressure bin; and finally, the surface is cleaned to remove floating color, and a layer of varnish is coated to ensure that the dyed plasticized specimen has a more bright color, is moisture-proof and wear-resistant, can be prevented from being oxidized, can be stored for a long time and is used for teaching and observation.
Description
Technical Field
The invention relates to the field of plasticized specimen dyeing, in particular to a method for dyeing blood vessels and nerves of a human plasticized specimen.
Background
The biological specimen is a real object for teaching, scientific research or display and observation of subjects such as biology and the like by processing the whole or part of an animal or a plant, keeping the original shape or characteristics of the animal or the plant, and storing the animal or the plant in a laboratory of a scientific research institution or school. At present, the common biological specimens for teaching and learning in schools are as follows: model specimen, stripping specimen, soaking bottled specimen.
The plasticizing specimen technology is applied to the history of about 20 years in the field of biological anatomical standards, and the biological plasticizing specimen produced by the plasticizing technology has the advantages of no toxicity, no smell, long-term preservation, convenient use and long-term preservation in the air. However, the whole plasticized specimen of a human body is grey white, the walking distribution of arteries, veins and nerves cannot be clearly distinguished, and although the existing specimen dyeing technology can distinguish the arteries, veins and nerves, the color is not bright and bright enough, the specimen cannot be stored for a long time easily, and the specimen is not beneficial to long-term observation and study.
Disclosure of Invention
The invention aims to provide a method for dyeing blood vessels and nerves of a human plasticized specimen, which aims to solve the problems that the dyed human plasticized specimen is not bright in color and is not easy to store for a long time.
In order to achieve the purpose, the invention adopts the following technical scheme: a method for staining blood vessels and nerves of a human plasticized specimen comprises the following steps:
1) preparation of a specimen: selecting a plasticized specimen needing to display the structures and the shapes of blood vessels and nerves;
2) preparing a coloring agent: selecting a required pigment toner, mixing the pigment toner with epoxy resin AB glue, adding absolute ethyl alcohol for dilution, and adjusting to a proper color depth;
3) pretreatment of a specimen: coating dimethylbenzene on the surfaces of blood vessels and nerves, and oxidizing and bleaching the specimen by peroxide after the dimethylbenzene is completely volatilized;
4) dyeing: uniformly coating the prepared different dyes on the surfaces of blood vessels and nerves by using a small brush pen;
5) fixation: placing the dyed plasticized specimen in a vacuum pressure bin for solidification;
6) cleaning and drying: and putting the dyed plasticized human body specimen into flowing water to wash off surface floating color, uniformly coating a layer of varnish with a brush pen after ensuring no color fading, and naturally drying to obtain a dyed finished product.
Preferably, the pigment toner in the step 2) is red, yellow and blue pigment toner ground by natural ore, and is screened by a 300-500-mesh sieve for use.
Further preferably, the pigment toner and the epoxy resin AB glue in the step 2) are mixed according to the volume ratio (3-6): (1.5-3): (0.8-1.2) mixing to obtain the product.
Further preferably, the peroxide in the step 3) is H adjusted to pH between 10 and 11 by adding sodium silicate2O2。
Further preferably, in step 4), arterial blood vessels are stained red, venous blood vessels are stained blue, and nerves are stained yellow.
Preferably, in the step 5), the stained specimen is placed in a vacuum pressure chamber at 50-60 ℃ for 20-30 hours, and the pressure of the pressure chamber is gradually increased from 0.1kpa to 1 kp.
Further preferably, the varnish in step 6) is a novolac, an alkyd varnish, a polyurethane varnish or an acrylic varnish.
The invention has the beneficial effects that:
a method for dyeing blood vessels and nerves of a human plasticized specimen comprises the steps of pretreating the plasticized specimen, dyeing by using a dye, respectively dyeing arterial blood vessels, venous blood vessels and nerves of the plasticized specimen, and finally fixing a pigment on the specimen by using epoxy resin AB glue.
The dyeing specimen prepared in the application of the invention is nontoxic and tasteless, can be placed in the air for long-term storage for a long time, is convenient for long-term storage, saves teaching cost for schools after being used for many times, can be placed in the air for a long time, is free from insects, has no pungent smell, is easy to store, has a complete and attractive structure, provides a healthy product for users, and has wide popularization and application values.
Drawings
FIG. 1 is a photograph of neurostaining of blood vessels in the craniofacial plastic specimen in example 1;
FIG. 2 is a photograph showing the nerve staining of the blood vessel of the pot plasticized specimen in example 2.
Detailed Description
The technical solution in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention.
Example 1: as shown in figure 1, the staining method for the blood vessels and nerves of the human plasticized specimen comprises the following manufacturing steps:
1) preparation of a specimen: and selecting a plasticized specimen needing to display the blood vessel, the nerve structure and the shape, wherein the plasticized specimen is selected from the head and face part.
2) Preparing a coloring agent: selecting a required pigment toner, mixing the pigment toner with epoxy resin AB glue, selecting red, yellow and blue pigment toners which are ground by natural ores for the pigment toner, and screening by adopting a 300-mesh screen for use, wherein the ratio of the pigment toner to the epoxy resin AB glue is 3: 1.5: 0.8, then adding a small amount of absolute ethyl alcohol for dilution, and adjusting to a proper color depth;
3) pretreatment of a specimen: coating dimethylbenzene on the surfaces of blood vessels and nerves, removing redundant glue on the surfaces of the blood vessels and the nerves, and oxidizing and bleaching the dimethylbenzene by using peroxide after the dimethylbenzene is completely volatilized, wherein the peroxide is H with the pH value adjusted to 10-11 by adding sodium silicate2O2When the oxidized and bleached blood vessel nerve is subjected to subsequent dyeing, the dye can better permeate into the blood vessel and the nerve;
4) dyeing: uniformly coating prepared different dyes on the surfaces of blood vessels and nerves by using a small brush pen, wherein the arterial blood vessels are dyed red, the venous blood vessels are dyed blue, and the nerves are dyed yellow;
5) fixation: the dyed human plasticized specimen is placed in a vacuum pressure chamber at 50 ℃ for 20 hours, liquid dye on the surfaces of blood vessels and nerves permeates into the tissue of the specimen by extrusion, and meanwhile, absolute ethyl alcohol volatilizes, the epoxy resin AB glue begins to solidify, and the pigment can be better fixed on the specimen. Starting the pressure from 0.1kpa, and slowly adjusting the pressure until the pressure reaches 1 kpa;
6) cleaning and drying: and putting the dyed plasticized human body specimen into flowing water to wash off surface floating color, uniformly coating a layer of varnish which is phenolic varnish with a writing brush after ensuring no color fading, and naturally airing to obtain a dyed finished product.
Example 2: as shown in figure 1, the staining method for the blood vessels and nerves of the human plasticized specimen comprises the following manufacturing steps:
1) preparation of a specimen: selecting a plasticized specimen needing to display the structures of blood vessels and nerves and the shape, wherein the plasticized specimen of the basin part is selected in the embodiment;
2) preparing a coloring agent: selecting a required pigment toner, mixing the pigment toner with epoxy resin AB glue, selecting red, yellow and blue pigment toners which are ground by natural ores for the pigment toner, and screening by adopting a 300-mesh screen for use, wherein the ratio of the pigment toner to the epoxy resin AB glue is 5: 2.5: 1, adding a small amount of absolute ethyl alcohol for dilution, and adjusting to a proper color depth;
3) pretreatment of a specimen: coating dimethylbenzene on the surfaces of blood vessels and nerves, removing redundant glue on the surfaces of the blood vessels and the nerves, and oxidizing and bleaching the dimethylbenzene by using peroxide after the dimethylbenzene is completely volatilized, wherein the peroxide is H with the pH value adjusted to 10-11 by adding sodium silicate2O2When the oxidized and bleached blood vessel nerve is subjected to subsequent dyeing, the dye can better permeate into the blood vessel and the nerve;
4) dyeing: uniformly coating prepared different dyes on the surfaces of blood vessels and nerves by using a small brush pen, wherein the arterial blood vessels are dyed red, the venous blood vessels are dyed blue, and the nerves are dyed yellow;
5) fixation: the dyed human plasticized specimen is placed in a vacuum pressure chamber at 50 ℃ for 24 hours, liquid dye on the surfaces of blood vessels and nerves permeates into the tissue of the specimen by extrusion, and meanwhile, absolute ethyl alcohol volatilizes, the epoxy resin AB glue begins to solidify, and the pigment can be better fixed on the specimen. Starting the pressure from 0.1kpa, and slowly adjusting the pressure until the pressure reaches 1 kpa;
6) cleaning and drying: and putting the dyed plasticized human body specimen into flowing water to wash off surface floating color, uniformly coating a layer of varnish which is phenolic varnish with a writing brush after ensuring no color fading, and naturally airing to obtain a dyed finished product.
Example 3: a method for dyeing blood vessels and nerves of a human plasticized specimen comprises the following manufacturing steps:
1) preparation of a specimen: and selecting a plasticized specimen needing to display the blood vessel, the nerve structure and the shape, wherein the plasticized specimen is selected from the head and face part.
2) Preparing a coloring agent: selecting a required pigment toner, mixing the pigment toner with epoxy resin AB glue, selecting red, yellow and blue pigment toners which are ground by natural ores for the pigment toner, and screening by adopting a 300-mesh screen for use, wherein the ratio of the pigment toner to the epoxy resin AB glue is 6: 3: 1.2, adding a small amount of absolute ethyl alcohol for dilution, and adjusting to a proper color depth;
3) pretreatment of a specimen: coating xylene on blood vessel and nerve surface, removing excessive glue on blood vessel and nerve surface, volatilizing xylene completely, and adding H2O2Oxidative bleaching of in which H2O2Is at 3g/L of H2O2Sodium silicate is added to adjust the PH value to 10-11, and the dye can better permeate into blood vessels and nerves when the oxidized and bleached blood vessel nerves are subjected to subsequent dyeing;
4) dyeing: uniformly coating prepared different dyes on the surfaces of blood vessels and nerves by using a small brush pen, wherein the arterial blood vessels are dyed red, the venous blood vessels are dyed blue, and the nerves are dyed yellow;
5) fixation: the dyed plasticized specimen of the human body is placed in a vacuum pressure chamber at 60 ℃ for 30 hours, liquid dye on the surfaces of blood vessels and nerves permeates into tissue of the specimen by extrusion, and meanwhile, absolute ethyl alcohol volatilizes, the epoxy resin AB glue begins to solidify, and the pigment can be better fixed on the specimen. The pressure is started from 0.1kpa, and the pressure is slowly adjusted until 1kpa is reached.
6) Cleaning and drying: and putting the dyed plasticized human body specimen into flowing water to wash off surface floating color, uniformly coating a layer of varnish which is phenolic varnish with a writing brush after ensuring no color fading, and naturally airing to obtain a dyed finished product.
The varnish in other embodiments may also be an alkyd varnish, a polyurethane varnish, an acrylic varnish, or the like.
The present invention is not limited to the above-mentioned preferred embodiments, and any other products in various forms can be obtained by anyone in the light of the present invention, but any changes in the shape or structure thereof, which have the same or similar technical solutions as those of the present application, fall within the protection scope of the present invention.
Claims (7)
1. A method for dyeing blood vessels and nerves of a human plasticized specimen is characterized by comprising the following steps: the method comprises the following steps:
1) preparation of a specimen: selecting a plasticized specimen needing to display the structures and the shapes of blood vessels and nerves;
2) preparing a coloring agent: selecting a required pigment toner, mixing the pigment toner with epoxy resin AB glue, adding absolute ethyl alcohol for dilution, and adjusting to a proper color depth;
3) pretreatment of a specimen: coating dimethylbenzene on the surfaces of blood vessels and nerves, and oxidizing and bleaching the specimen by peroxide after the dimethylbenzene is completely volatilized;
4) dyeing: uniformly coating the prepared different dyes on the surfaces of blood vessels and nerves by using a small brush pen;
5) fixation: placing the dyed plasticized specimen in a vacuum pressure bin for solidification;
6) cleaning and drying: and putting the dyed plasticized human body specimen into flowing water to wash off surface floating color, uniformly coating a layer of varnish with a brush pen after ensuring no color fading, and naturally drying to obtain a dyed finished product.
2. The method for staining human plasticized specimen vessels and nerves according to claim 1, wherein: in the step 2), the pigment toner is red, yellow and blue pigment toner ground by natural ore, and is screened by a 300-500-mesh sieve for use.
3. The method for staining the blood vessels and the nerves of the human plasticized specimen according to claim 1, wherein the pigment toner and the epoxy resin AB glue in the step 2) are mixed in a volume ratio of (3-6): (1.5-3): (0.8-1.2) mixing to obtain the product.
4. The method for staining human plasticized specimen vessels and nerves according to claim 1, wherein: the peroxide in the step 3) is H adjusted to pH between 10 and 11 by adding sodium silicate2O2。
5. The method for staining human plasticized specimen vessels and nerves according to claim 1, wherein: in the step 4), arterial blood vessels are stained red, venous blood vessels are stained blue, and nerves are stained yellow.
6. The method for staining human plasticized specimen vessels and nerves according to claim 1, wherein: in the step 5), the stained specimen is placed in a vacuum pressure chamber at the temperature of 50-60 ℃ for 20-30 hours, and the pressure of the pressure chamber is slowly increased from 0.1kpa to 1 kp.
7. The method for staining human plasticized specimen vessels and nerves according to claim 1, wherein: the varnish in step 6) is a novolac, an alkyd varnish, a polyurethane varnish or an acrylic varnish.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110041582.8A CN112903400B (en) | 2021-01-13 | 2021-01-13 | Method for dyeing blood vessels and nerves of human plasticized specimen |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202110041582.8A CN112903400B (en) | 2021-01-13 | 2021-01-13 | Method for dyeing blood vessels and nerves of human plasticized specimen |
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| Publication Number | Publication Date |
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| CN112903400A true CN112903400A (en) | 2021-06-04 |
| CN112903400B CN112903400B (en) | 2023-03-14 |
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Cited By (1)
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|---|---|---|---|---|
| CN113933132A (en) * | 2021-10-12 | 2022-01-14 | 河南中博科技有限公司 | Plasticized specimen muscle staining agent and staining method |
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| CN113933132A (en) * | 2021-10-12 | 2022-01-14 | 河南中博科技有限公司 | Plasticized specimen muscle staining agent and staining method |
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| Publication number | Publication date |
|---|---|
| CN112903400B (en) | 2023-03-14 |
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