CN103404506B - Method for preparing specimen of muscle of whole adult fox - Google Patents
Method for preparing specimen of muscle of whole adult fox Download PDFInfo
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- CN103404506B CN103404506B CN201310286985.4A CN201310286985A CN103404506B CN 103404506 B CN103404506 B CN 103404506B CN 201310286985 A CN201310286985 A CN 201310286985A CN 103404506 B CN103404506 B CN 103404506B
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Abstract
The invention discloses a method for preparing a specimen of muscle of a whole adult fox. The method comprises the steps of selecting a dead body of an anesthetized adult fox killed by perfusion fixation; then peeling the adult fox, completely separating the muscle of the whole fox; cleaning the separated muscle of the fox with water, soaking the muscle of the fox by using a 80 percent of alcohol water solution for two days, and then taking out the muscle of the fox, washing the muscle of the fox with water, soaking the muscle of the fox by using a mixed solution of absolute ethyl alcohol and dimethylbenzene with a volume ratio of 1.3:2 for two days and then taking the muscle of the fox out, airing the muscle of the fox in a dark ventilating place for 7-8 days, then soaking the muscle of the fox in an aqueous hydrogen peroxide solution with a mass percent of 4.4 percent for 8 days and then taking out the muscle of the fox, cleaning the muscle of the fox by using clear water, and airing; then fixing the muscle on a glass plate by using glue water to ensure that the muscle is kept in a gesture that the fox stands, then placing the muscle of the fox in a specimen jar to prepare the specimen of muscle of whole adult fox. According to the method disclosed by the invention, the preparation method is simple, and the cost is low; the prepared specimen of muscle of whole adult fox is easy to store, complete and attractive in structure, and clear and natural in form and position.
Description
Technical field
The present invention relates to a kind of Slide processing, specifically the whole-body muscle Slide processing of a kind of adult fox.
Background technology
Zoological specimens, be keep former state in kind or through arrangement can for referential when study, research can individuality representatively in one species, also can be used as the important references of universal publicity of scientific research, teaching and Sci-tech Knowledge.Scientific value, ornamental value and reserve value that what animal and tissue specimen contained can not be substituted, other arts work of erect image equally enter into family, become the indoor new ornament of people.One makes exquisite sample and not only brings people's knowledge, also brings the enjoyment of people's art simultaneously.Perfect animal muscle sample is a vivid practical teaching aid, can be again a consummate art work, it represent colleges and universities, a subject development level and grasp the professional skill of this disciplinary study person.Preparation of specimen is also a research work, in manufacturing process, can constantly discover problems and solve them, and creates new technology, can broaden one's knowledge simultaneously, tempers thinking and the manipulative ability of participant.Be made into whole muscle specimen, whole-body muscle structure can be demonstrated, sample structure is attractive in appearance, form knows nature, not only " Zootomy " teaching in as directly perceived, vivid teaching aid, and can allow students' participation in manufacturing process, allow student understand and grasp preparation of specimen's process, simultaneously the starting of training student, beat one's brains ability.Contribute to, to the understanding of student to animal whole-body muscle overview, grasping the contour structures of whole-body muscle, good help is all played to the understanding of the Name & Location relation of muscle, contribute to improving teaching efficiency.
Summary of the invention
The present invention seeks to as solving the problems of the technologies described above, providing the whole-body muscle Slide processing of a kind of adult fox, its preparation method is simple, and prepared sample can demonstrate the whole-body muscle of adult fox, and structure is attractive in appearance, and form knows nature.
A whole-body muscle Slide processing for adult fox, its concrete preparation process comprises:
After step one, selection anesthesia, through the corpse of the adult fox that perfusion fixation is put to death;
Step 2, peeling: faced upward by fox corpse and be put on autopsy table, cut the osculum that diameter is 1.5 ~ 2cm respectively with operating scissors inside front wrist joint and hind leg tarsal joint, then respectively from cutting off fox skin skin inside cut osculum to anus front; Then be separated fox skin skin, by the method for drill drift, skin sloughed to neck, become dermatotome housing type outwardly; Cut off the auricular cartilage of ear again, be separated eye and mouth skin of lip, by complete for skin stripping, finally gill;
The whole-body muscle of step 3, separation fox: the muscle isolating fox whole body with scalpel; Concrete operation method is: isolate scalp flesh, musculus cutaneus colli and trunk musculus cutaneus after fascia superficialis under stripping head, neck and body skin.Wipe out manadesma and connective tissue along muscle Germ distribution, isolate the facial muscle of head, masseter and temporalis; At neck portion from cucullaris, omoplate transverse process flesh, longest muscle of neck, arm head flesh, musculus sternocephalicus, splenius, semispinalis and musculus hyoideus.At trunk, after peeling off manadesma, connective tissue and blood vessel along the boundary of muscle, terminal, to be separated on latissimus dorsi, chest muscle, veutro serratus, intercostal muscle, levator muscles of ribs, scalenus, musculus longus colli, omoplate the muscle such as flesh, latissimus dorsi, intercostal muscle, back of the body waist eye muscle, iliocostalis, greater psoas muscle, smaller psoas muscle, iliocostalis ditch, dorsal part serratus, external intercostal muscles, intercostals interni, musculus obliquus externus abdominis, oblique, rectus aabdominis, musculus trasversus abdomins.At forelimb, be separated supraspinous muscle, infraspinous muscle, deltoid muscle, subscapular muscle, musculus teres major, arm flesh, antebrachial fascia tensor muscle, arm triceps, arm flesh, arm biceps, forearm, forward foot in a step flesh; At hind leg, be separated gluteus, musculus glutaeus superficialis, gluteus medius, musculus glutaeus profundus, thigh flesh, stern biceps muscle of thigh, semitendinosus, semimembranosus, tensor fasciae late muscle, quadriceps muscle of thigh, gracilis, adductor, shank and rear foot flesh etc.;
Step 4, degreasing: by isolated fox whole-body muscle, rinse well with water, be placed in infuse container, then add the ethanol water that mass percent is 80%, makes ethanol water not have fox muscle completely, and soak two days later, taking-up water is rinsed well; Again put back to infuse container, adding volume ratio is again the absolute ethyl alcohol of 1.3:2 and the mixed solution of dimethylbenzene, absolute ethyl alcohol and dimethylbenzene mixed solution is made not to have fox muscle completely, changed every 16 hours and once soak the absolute ethyl alcohol and dimethylbenzene mixed solution that volume ratio used is 1.3:2, soak and take out two days later; Or isolated fox whole-body muscle is placed in infuse container, pours gasoline into, airtight, soak and carry out degreasing in 4 days.In the operating process of degreasing, to obturage infuse container with Polypropylence Sheet, prevent solution evaporation.
Process after step 5, degreasing: the fox muscle after degreasing is placed on dark ventilation and dries in the air 7 ~ 8 days, then by the fox muscle mass percent dried well be 6% detergent solution soak 1 ~ 2h, then use clean water three times;
Step 6, above-mentioned steps five is processed after fox muscle insert the aqueous hydrogen peroxide solution that mass percent is 4.4%, soak and take out after 8 days, rinse well with clear water, dry;
Step 7, with glue by fixing for muscle on a glass, posture when making muscle keep fox to stand, then puts in specimen jar, namely prepares the whole-body muscle sample of adult fox.
Beneficial effect is:
1, the present invention grows up the whole-body muscle Slide processing of fox, and preparation method is simple, easy, with low cost; Adopt ethanol water, absolute ethyl alcohol and dimethylbenzene or gasoline to carry out ungrease treatment to fox muscle, degreasing is thorough, can prevent from using permanent and preserve, and slowly oozing out of fox muscle grease, pollutes mouldy, makes sample impaired, hinders to use and attractive in appearance.Adopt the mass percent of hydrogen oxide to be that after the dilution of 4.4%, hydrogen peroxide is bleached, effect is better and to fox whole-body muscle sample not damaged.
2, the present invention grows up the whole-body muscle sample of the whole-body muscle Slide processing of fox, prepared fox, is easy to preserve, structural integrity is attractive in appearance, morphologic localization knows nature, and whole-body muscle is all clearly illustrated, each block muscle represents all well and is fixed on the physical slot of chicken body.Solve the problem of very difficult clear resolution fox whole-body muscle.Muscle is the emphasis in Teaching of Anatomy, and the muscle in Systematic Anatomy teaching is mainly theoretical, and topologic Students in Teaching does not dissect whole muscle; The present invention preferably resolves this difficult problem.Excite again the learning interest of student, improve teaching efficiency.
Embodiment
A whole-body muscle Slide processing for adult fox, its concrete preparation process comprises:
After step one, selection anesthesia, through the corpse of the adult fox that perfusion fixation is put to death;
Step 2, peeling: faced upward by fox corpse and be put on autopsy table, cut the osculum that diameter is 1.5 ~ 2cm respectively with operating scissors inside front wrist joint and hind leg tarsal joint, then respectively from cutting off fox skin skin inside cut osculum to anus front; Then be separated fox skin skin, by the method for drill drift, skin sloughed to neck, become dermatotome housing type outwardly; Cut off the auricular cartilage of ear again, be separated eye and mouth skin of lip, by complete for skin stripping, finally gill;
The whole-body muscle of step 3, separation fox: the muscle isolating fox whole body with scalpel; Concrete operation method is: isolate scalp flesh, musculus cutaneus colli and trunk musculus cutaneus after fascia superficialis under stripping head, neck and body skin.Wipe out manadesma and connective tissue along muscle Germ distribution, isolate the facial muscle of head, masseter and temporalis; At neck portion from cucullaris, omoplate transverse process flesh, longest muscle of neck, arm head flesh, musculus sternocephalicus, splenius, semispinalis and musculus hyoideus.At trunk, after peeling off manadesma, connective tissue and blood vessel along the boundary of muscle, terminal, to be separated on latissimus dorsi, chest muscle, veutro serratus, intercostal muscle, levator muscles of ribs, scalenus, musculus longus colli, omoplate the muscle such as flesh, latissimus dorsi, intercostal muscle, back of the body waist eye muscle, iliocostalis, greater psoas muscle, smaller psoas muscle, iliocostalis ditch, dorsal part serratus, external intercostal muscles, intercostals interni, musculus obliquus externus abdominis, oblique, rectus aabdominis, musculus trasversus abdomins.At forelimb, be separated supraspinous muscle, infraspinous muscle, deltoid muscle, subscapular muscle, musculus teres major, arm flesh, antebrachial fascia tensor muscle, arm triceps, arm flesh, arm biceps, forearm, forward foot in a step flesh; At hind leg, be separated gluteus, musculus glutaeus superficialis, gluteus medius, musculus glutaeus profundus, thigh flesh, stern biceps muscle of thigh, semitendinosus, semimembranosus, tensor fasciae late muscle, quadriceps muscle of thigh, gracilis, adductor, shank and rear foot flesh etc.;
Step 4, degreasing: by isolated fox whole-body muscle, rinse well with water, be placed in infuse container, then add the ethanol water that mass percent is 80%, makes ethanol water not have fox muscle completely, and soak two days later, taking-up water is rinsed well; Again put back to infuse container, adding volume ratio is again the absolute ethyl alcohol of 1.3:2 and the mixed solution of dimethylbenzene, absolute ethyl alcohol and dimethylbenzene mixed solution is made not to have fox muscle completely, changed every 16 hours and once soak the absolute ethyl alcohol and dimethylbenzene mixed solution that volume ratio used is 1.3:2, soak and take out two days later; Or isolated fox whole-body muscle is placed in infuse container, pours gasoline into, airtight, soak and carry out degreasing in 4 days.In the operating process of degreasing, to obturage infuse container with Polypropylence Sheet, prevent solution evaporation.
Process after step 5, degreasing: the fox muscle after degreasing is placed on dark ventilation and dries in the air 7 ~ 8 days, then by the fox muscle mass percent dried well be 6% detergent solution soak 1 ~ 2h, then use clean water three times;
Step 6, above-mentioned steps five is processed after fox muscle insert the aqueous hydrogen peroxide solution that mass percent is 4.4%, soak and take out after 8 days, rinse well with clear water, dry;
Step 7, with glue by fixing for muscle on a glass, posture when making muscle keep fox to stand, then puts in specimen jar, namely prepares the whole-body muscle sample of adult fox.
Following examples can make the present invention of those skilled in the art's complete understanding, but do not limit the present invention in any way.
embodiment 1
A whole-body muscle Slide processing for adult fox, its concrete preparation process comprises:
Step one, the adult fox one selecting build plentiful and substantial, healthy and strong, claim its body weight;
Step 2, solution preparation: the body weight according to fox takes yellow Jackets, standard is that every 1kg fox takes yellow Jackets 45mg, is dissolved in stroke-physiological saline solution and is made into the Nembutal sodium solution that mass fraction is 3%, for subsequent use; The mass percent with distilled water formalin solution being diluted to formaldehyde is the solution of 7%, prepares the formalin solution after dilution, for subsequent use;
Step 3, perfusion fixation: draw the Nembutal sodium solution prepared by above-mentioned steps two with syringe, adopt intraperitoneal injection anesthesia fox; Then open at the skin place of fox jugular vein ditch the mouth that diameter is 3 cm with scalpel, expose arteria carotis communis; On arteria carotis communis, the osculum that diameter is 0.3cm is cut with operating scissors, perfusion syringe needle is inserted in osculum, fix with haemostatic clamp, quick filling 3000ml physiological saline, treat that blood is rinsed well, after namely liver bleaches, pour into the formalin solution after 5000 ml dilutions, first quick and back slow, about two hours are continued;
Step 4, peeling: faced upward by fox corpse and be put on autopsy table, cut the osculum that diameter is 1.5cm respectively with operating scissors inside front wrist joint and hind leg tarsal joint, then respectively from cutting off fox skin skin inside cut osculum to anus front; Then be separated fox skin skin, by the method for drill drift, skin sloughed to neck, become dermatotome housing type outwardly; Cut off the auricular cartilage of ear again, be separated eye and mouth skin of lip, by complete for skin stripping, finally gill;
The whole-body muscle of step 5, separation fox: the muscle isolating fox whole body with scalpel; Concrete operation method is: isolate scalp flesh, musculus cutaneus colli and trunk musculus cutaneus after fascia superficialis under stripping head, neck and body skin.Wipe out manadesma and connective tissue along muscle Germ distribution, isolate the facial muscle of head, masseter and temporalis; At neck portion from cucullaris, omoplate transverse process flesh, longest muscle of neck, arm head flesh, musculus sternocephalicus, splenius, semispinalis and musculus hyoideus.At trunk, after peeling off manadesma, connective tissue and blood vessel along the boundary of muscle, terminal, to be separated on latissimus dorsi, chest muscle, veutro serratus, intercostal muscle, levator muscles of ribs, scalenus, musculus longus colli, omoplate the muscle such as flesh, latissimus dorsi, intercostal muscle, back of the body waist eye muscle, iliocostalis, greater psoas muscle, smaller psoas muscle, iliocostalis ditch, dorsal part serratus, external intercostal muscles, intercostals interni, musculus obliquus externus abdominis, oblique, rectus aabdominis, musculus trasversus abdomins.At forelimb, be separated supraspinous muscle, infraspinous muscle, deltoid muscle, subscapular muscle, musculus teres major, arm flesh, antebrachial fascia tensor muscle, arm triceps, arm flesh, arm biceps, forearm, forward foot in a step flesh; At hind leg, be separated gluteus, musculus glutaeus superficialis, gluteus medius, musculus glutaeus profundus, thigh flesh, stern biceps muscle of thigh, semitendinosus, semimembranosus, tensor fasciae late muscle, quadriceps muscle of thigh, gracilis, adductor, shank and rear foot flesh etc.;
Step 6, degreasing: by isolated fox whole-body muscle, rinse well with water, be placed in infuse container, then add the ethanol water that mass percent is 80%, makes ethanol water not have fox muscle completely, and soak two days later, taking-up water is rinsed well; Again put back to infuse container, adding volume ratio is again the absolute ethyl alcohol of 1.3:2 and the mixed solution of dimethylbenzene, absolute ethyl alcohol and dimethylbenzene mixed solution is made not to have fox muscle completely, changed every 16 hours and once soak the absolute ethyl alcohol and dimethylbenzene mixed solution that volume ratio used is 1.3:2, soak and take out two days later; In the operating process of degreasing, to obturage infuse container with Polypropylence Sheet, prevent solution evaporation.
Process after step 7, degreasing: the fox muscle after degreasing is placed on dark ventilation and dries in the air 7 days, then by the fox muscle mass percent dried well be 6% detergent solution soak 1h, then use clean water three times;
Step 8, above-mentioned steps five is processed after fox muscle insert the aqueous hydrogen peroxide solution that mass percent is 4.4%, soak and take out after 8 days, rinse well with clear water, dry;
Step 9, with glue by fixing for muscle on a glass, posture when making muscle keep fox to stand, then puts in specimen jar, namely prepares the whole-body muscle sample of adult fox.
embodiment 2
A whole-body muscle Slide processing for adult fox, its concrete preparation process comprises:
Step one, the adult fox one selecting build plentiful and substantial, healthy and strong, claim its body weight;
Step 2, solution preparation: the body weight according to fox takes yellow Jackets, standard is that every 1kg fox takes yellow Jackets 45mg, is dissolved in stroke-physiological saline solution and is made into the Nembutal sodium solution that mass fraction is 3%, for subsequent use; The mass percent with distilled water formalin solution being diluted to formaldehyde is the solution of 7%, prepares the formalin solution after dilution, for subsequent use;
Step 3, perfusion fixation: draw the Nembutal sodium solution prepared by above-mentioned steps two with syringe, adopt intraperitoneal injection anesthesia fox; Then open at the skin place of fox jugular vein ditch the mouth that diameter is 3 cm with scalpel, expose arteria carotis communis; On arteria carotis communis, the osculum that diameter is 0.3cm is cut with operating scissors, perfusion syringe needle is inserted in osculum, fix with haemostatic clamp, quick filling 3000ml physiological saline, treat that blood is rinsed well, after namely liver bleaches, pour into the formalin solution after 5000 ml dilutions, first quick and back slow, about two hours are continued;
Step 4, peeling: faced upward by fox corpse and be put on autopsy table, cut the osculum that diameter is 2cm respectively with operating scissors inside front wrist joint and hind leg tarsal joint, then respectively from cutting off fox skin skin inside cut osculum to anus front; Then be separated fox skin skin, by the method for drill drift, skin sloughed to neck, become dermatotome housing type outwardly; Cut off the auricular cartilage of ear again, be separated eye and mouth skin of lip, by complete for skin stripping, finally gill;
The whole-body muscle of step 5, separation fox: the muscle isolating fox whole body with scalpel; Concrete operation method is: isolate scalp flesh, musculus cutaneus colli and trunk musculus cutaneus after fascia superficialis under stripping head, neck and body skin.Wipe out manadesma and connective tissue along muscle Germ distribution, isolate the facial muscle of head, masseter and temporalis; At neck portion from cucullaris, omoplate transverse process flesh, longest muscle of neck, arm head flesh, musculus sternocephalicus, splenius, semispinalis and musculus hyoideus.At trunk, after peeling off manadesma, connective tissue and blood vessel along the boundary of muscle, terminal, to be separated on latissimus dorsi, chest muscle, veutro serratus, intercostal muscle, levator muscles of ribs, scalenus, musculus longus colli, omoplate the muscle such as flesh, latissimus dorsi, intercostal muscle, back of the body waist eye muscle, iliocostalis, greater psoas muscle, smaller psoas muscle, iliocostalis ditch, dorsal part serratus, external intercostal muscles, intercostals interni, musculus obliquus externus abdominis, oblique, rectus aabdominis, musculus trasversus abdomins.At forelimb, be separated supraspinous muscle, infraspinous muscle, deltoid muscle, subscapular muscle, musculus teres major, arm flesh, antebrachial fascia tensor muscle, arm triceps, arm flesh, arm biceps, forearm, forward foot in a step flesh; At hind leg, be separated gluteus, musculus glutaeus superficialis, gluteus medius, musculus glutaeus profundus, thigh flesh, stern biceps muscle of thigh, semitendinosus, semimembranosus, tensor fasciae late muscle, quadriceps muscle of thigh, gracilis, adductor, shank and rear foot flesh etc.;
Step 6, degreasing: by isolated fox whole-body muscle, rinse well with water, be placed in infuse container, then add the ethanol water that mass percent is 80%, makes ethanol water not have fox muscle completely, and soak two days later, taking-up water is rinsed well; Again put back to infuse container, adding volume ratio is again the absolute ethyl alcohol of 1.3:2 and the mixed solution of dimethylbenzene, absolute ethyl alcohol and dimethylbenzene mixed solution is made not to have fox muscle completely, changed every 16 hours and once soak the absolute ethyl alcohol and dimethylbenzene mixed solution that volume ratio used is 1.3:2, soak and take out two days later; In the operating process of degreasing, to obturage infuse container with Polypropylence Sheet, prevent solution evaporation.
Process after step 7, degreasing: the fox muscle after degreasing is placed on dark ventilation and dries in the air 8 days, then by the fox muscle mass percent dried well be 6% detergent solution soak 2h, then use clean water three times;
Step 8, above-mentioned steps five is processed after fox muscle insert the aqueous hydrogen peroxide solution that mass percent is 4.4%, soak and take out after 8 days, rinse well with clear water, dry;
Step 9, with glue by fixing for muscle on a glass, posture when making muscle keep fox to stand, then puts in specimen jar, namely prepares the whole-body muscle sample of adult fox.
embodiment 3
A whole-body muscle Slide processing for adult fox, its concrete preparation process comprises:
Step one, the adult fox one selecting build plentiful and substantial, healthy and strong, claim its body weight;
Step 2, solution preparation: the body weight according to fox takes yellow Jackets, standard is that every 1kg fox takes yellow Jackets 45mg, is dissolved in stroke-physiological saline solution and is made into the Nembutal sodium solution that mass fraction is 3%, for subsequent use; The mass percent with distilled water formalin solution being diluted to formaldehyde is the solution of 7%, prepares the formalin solution after dilution, for subsequent use;
Step 3, perfusion fixation: draw the Nembutal sodium solution prepared by above-mentioned steps two with syringe, adopt intraperitoneal injection anesthesia fox; Then open at the skin place of fox jugular vein ditch the mouth that diameter is 3 cm with scalpel, expose arteria carotis communis; On arteria carotis communis, the osculum that diameter is 0.3cm is cut with operating scissors, perfusion syringe needle is inserted in osculum, fix with haemostatic clamp, quick filling 3000ml physiological saline, treat that blood is rinsed well, after namely liver bleaches, pour into the formalin solution after 5000 ml dilutions, first quick and back slow, about two hours are continued;
Step 4, peeling: faced upward by fox corpse and be put on autopsy table, cut the osculum that diameter is 1.8cm respectively with operating scissors inside front wrist joint and hind leg tarsal joint, then respectively from cutting off fox skin skin inside cut osculum to anus front; Then be separated fox skin skin, by the method for drill drift, skin sloughed to neck, become dermatotome housing type outwardly; Cut off the auricular cartilage of ear again, be separated eye and mouth skin of lip, by complete for skin stripping, finally gill;
The whole-body muscle of step 5, separation fox: the muscle isolating fox whole body with scalpel; Concrete operation method is: isolate scalp flesh, musculus cutaneus colli and trunk musculus cutaneus after fascia superficialis under stripping head, neck and body skin.Wipe out manadesma and connective tissue along muscle Germ distribution, isolate the facial muscle of head, masseter and temporalis; At neck portion from cucullaris, omoplate transverse process flesh, longest muscle of neck, arm head flesh, musculus sternocephalicus, splenius, semispinalis and musculus hyoideus.At trunk, after peeling off manadesma, connective tissue and blood vessel along the boundary of muscle, terminal, to be separated on latissimus dorsi, chest muscle, veutro serratus, intercostal muscle, levator muscles of ribs, scalenus, musculus longus colli, omoplate the muscle such as flesh, latissimus dorsi, intercostal muscle, back of the body waist eye muscle, iliocostalis, greater psoas muscle, smaller psoas muscle, iliocostalis ditch, dorsal part serratus, external intercostal muscles, intercostals interni, musculus obliquus externus abdominis, oblique, rectus aabdominis, musculus trasversus abdomins.At forelimb, be separated supraspinous muscle, infraspinous muscle, deltoid muscle, subscapular muscle, musculus teres major, arm flesh, antebrachial fascia tensor muscle, arm triceps, arm flesh, arm biceps, forearm, forward foot in a step flesh; At hind leg, be separated gluteus, musculus glutaeus superficialis, gluteus medius, musculus glutaeus profundus, thigh flesh, stern biceps muscle of thigh, semitendinosus, semimembranosus, tensor fasciae late muscle, quadriceps muscle of thigh, gracilis, adductor, shank and rear foot flesh etc.;
Step 6, degreasing: by isolated fox whole-body muscle, rinse well with water, be placed in infuse container, then add the ethanol water that mass percent is 80%, makes ethanol water not have fox muscle completely, and soak two days later, taking-up water is rinsed well; Again put back to infuse container, adding volume ratio is again the absolute ethyl alcohol of 1.3:2 and the mixed solution of dimethylbenzene, absolute ethyl alcohol and dimethylbenzene mixed solution is made not to have fox muscle completely, changed every 16 hours and once soak the absolute ethyl alcohol and dimethylbenzene mixed solution that volume ratio used is 1.3:2, soak and take out two days later; In the operating process of degreasing, to obturage infuse container with Polypropylence Sheet, prevent solution evaporation.
Process after step 7, degreasing: the fox muscle after degreasing is placed on dark ventilation and dries in the air 7 days, then by the fox muscle mass percent dried well be 6% detergent solution soak 1.5h, then use clean water three times;
Step 8, above-mentioned steps five is processed after fox muscle insert the aqueous hydrogen peroxide solution that mass percent is 4.4%, soak and take out after 8 days, rinse well with clear water, dry;
Step 9, with glue by fixing for muscle on a glass, posture when making muscle keep fox to stand, then puts in specimen jar, namely prepares the whole-body muscle sample of adult fox.
embodiment 4
A whole-body muscle Slide processing for adult fox, its concrete preparation process comprises:
Step one, the adult fox one selecting build plentiful and substantial, healthy and strong, claim its body weight;
Step 2, solution preparation: the body weight according to fox takes yellow Jackets, standard is that every 1kg fox takes yellow Jackets 45mg, is dissolved in stroke-physiological saline solution and is made into the Nembutal sodium solution that mass fraction is 3%, for subsequent use; The mass percent with distilled water formalin solution being diluted to formaldehyde is the solution of 7%, prepares the formalin solution after dilution, for subsequent use;
Step 3, perfusion fixation: draw the Nembutal sodium solution prepared by above-mentioned steps two with syringe, adopt intraperitoneal injection anesthesia fox; Then open at the skin place of fox jugular vein ditch the mouth that diameter is 3 cm with scalpel, expose arteria carotis communis; On arteria carotis communis, the osculum that diameter is 0.3cm is cut with operating scissors, perfusion syringe needle is inserted in osculum, fix with haemostatic clamp, quick filling 3000ml physiological saline, treat that blood is rinsed well, after namely liver bleaches, pour into the formalin solution after 5000 ml dilutions, first quick and back slow, about two hours are continued;
Step 4, peeling: faced upward by fox corpse and be put on autopsy table, cut the osculum that diameter is 1.7cm respectively with operating scissors inside front wrist joint and hind leg tarsal joint, then respectively from cutting off fox skin skin inside cut osculum to anus front; Then be separated fox skin skin, by the method for drill drift, skin sloughed to neck, become dermatotome housing type outwardly; Cut off the auricular cartilage of ear again, be separated eye and mouth skin of lip, by complete for skin stripping, finally gill;
The whole-body muscle of step 5, separation fox: the muscle isolating fox whole body with scalpel; Concrete operation method is: isolate scalp flesh, musculus cutaneus colli and trunk musculus cutaneus after fascia superficialis under stripping head, neck and body skin.Wipe out manadesma and connective tissue along muscle Germ distribution, isolate the facial muscle of head, masseter and temporalis; At neck portion from cucullaris, omoplate transverse process flesh, longest muscle of neck, arm head flesh, musculus sternocephalicus, splenius, semispinalis and musculus hyoideus.At trunk, after peeling off manadesma, connective tissue and blood vessel along the boundary of muscle, terminal, to be separated on latissimus dorsi, chest muscle, veutro serratus, intercostal muscle, levator muscles of ribs, scalenus, musculus longus colli, omoplate the muscle such as flesh, latissimus dorsi, intercostal muscle, back of the body waist eye muscle, iliocostalis, greater psoas muscle, smaller psoas muscle, iliocostalis ditch, dorsal part serratus, external intercostal muscles, intercostals interni, musculus obliquus externus abdominis, oblique, rectus aabdominis, musculus trasversus abdomins.At forelimb, be separated supraspinous muscle, infraspinous muscle, deltoid muscle, subscapular muscle, musculus teres major, arm flesh, antebrachial fascia tensor muscle, arm triceps, arm flesh, arm biceps, forearm, forward foot in a step flesh; At hind leg, be separated gluteus, musculus glutaeus superficialis, gluteus medius, musculus glutaeus profundus, thigh flesh, stern biceps muscle of thigh, semitendinosus, semimembranosus, tensor fasciae late muscle, quadriceps muscle of thigh, gracilis, adductor, shank and rear foot flesh etc.;
Step 6, degreasing: by isolated fox whole-body muscle, rinse well with water, be placed in infuse container, pour gasoline into, airtight, soak and carry out degreasing in 4 days.In the operating process of degreasing, to obturage infuse container with Polypropylence Sheet, prevent solution evaporation.
Process after step 7, degreasing: the fox muscle after degreasing is placed on dark ventilation and dries in the air 8 days, then by the fox muscle mass percent dried well be 6% detergent solution soak 2h, then use clean water three times;
Step 8, above-mentioned steps five is processed after fox muscle insert the aqueous hydrogen peroxide solution that mass percent is 4.4%, soak and take out after 8 days, rinse well with clear water, dry;
Step 9, with glue by fixing for muscle on a glass, posture when making muscle keep fox to stand, then puts in specimen jar, namely prepares the whole-body muscle sample of adult fox.
Claims (2)
1. a whole-body muscle Slide processing for adult fox, is characterized in that: its concrete preparation process comprises:
After step one, selection anesthesia, through the corpse of the adult fox that perfusion fixation is put to death;
Step 2, peeling: faced upward by fox corpse and be put on autopsy table, cut the osculum that diameter is 1.5 ~ 2cm respectively with operating scissors inside front wrist joint and hind leg tarsal joint, then respectively from cutting off fox skin skin inside cut osculum to anus front; Then be separated fox skin skin, by the method for drill drift, skin sloughed to neck, become dermatotome housing type outwardly; Cut off the auricular cartilage of ear again, be separated eye and mouth skin of lip, by complete for skin stripping, finally gill;
The whole-body muscle of step 3, separation fox: the muscle isolating fox whole body with scalpel;
Step 4, degreasing: by isolated fox muscle specimen, rinse well with water, be placed in infuse container, then add the ethanol water that mass percent is 80%, makes ethanol water not have fox muscle completely, and soak two days later, taking-up water is rinsed well; Again put back to infuse container, adding volume ratio is again the absolute ethyl alcohol of 1.3:2 and the mixed solution of dimethylbenzene, absolute ethyl alcohol and dimethylbenzene mixed solution is made not to have fox muscle completely, changed every 16 hours and once soak the absolute ethyl alcohol and dimethylbenzene mixed solution that volume ratio used is 1.3:2, soak and take out two days later;
Process after step 5, degreasing: the fox muscle after degreasing is placed on dark ventilation and dries in the air 7 ~ 8 days, then by the fox muscle mass percent dried well be 6% detergent solution soak 1 ~ 2h, then use clean water three times;
Step 6, above-mentioned steps five is processed after fox muscle insert the aqueous hydrogen peroxide solution that mass percent is 4.4%, soak and take out after 8 days, rinse well with clear water, dry;
Step 7, with glue by fixing for muscle on a glass, posture when making muscle keep fox to stand, then puts in specimen jar, namely prepares the whole-body muscle sample of adult fox;
In the operating process of described step 4 degreasing, to obturage infuse container with Polypropylence Sheet, prevent solution evaporation.
2. the whole-body muscle Slide processing of a kind of adult fox as claimed in claim 1, it is characterized in that: the degreasing method in described step 4 also can adopt: isolated fox muscle specimen is placed in infuse container, pour gasoline into, airtight, soak and carry out degreasing in 4 days.
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| CN112419856A (en) * | 2020-11-30 | 2021-02-26 | 河南中博生物塑化科技有限公司 | Preparation method of specimen for cardiac interventional operation |
| CN115945442A (en) * | 2022-12-19 | 2023-04-11 | 南京龙德生物科技有限公司 | Hydrogen peroxide soaking and flushing process for bone tissues |
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