CN103426355B - The whole-body muscle Slide processing of a kind of adult otter - Google Patents
The whole-body muscle Slide processing of a kind of adult otter Download PDFInfo
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Abstract
A muscle specimen method for making for adult otter, after selecting anesthesia, through the corpse of the adult otter that perfusion fixation is put to death; Then peeling is carried out to adult otter, and completely isolate otter whole-body muscle; After isolated otter muscle water is cleaned up, with the ethanol water of 80%, soak two days later, taking-up water is rinsed well, then is absolute ethyl alcohol and the dimethylbenzene mixed solution of 1:2 by volume ratio, soak and take out two days later, be placed on dark ventilation to dry in the air 6-7 days, be placed in the hydrogen peroxide after dilution afterwards, soak and take out after 2-5 days, rinse well with clear water, dry; Then fixed on a glass by muscle with glue, posture when making muscle keep otter to stand, then puts in specimen jar, namely prepares the whole-body muscle sample of adult otter.Preparation method of the present invention is simple, with low cost, the muscle specimen of prepared adult otter, is easy to preserve, and structural integrity is attractive in appearance, and morphologic localization knows nature.
Description
Technical field
The present invention relates to a kind of Slide processing, specifically the whole-body muscle Slide processing of a kind of adult otter.
Background technology
Zoological specimens, be keep former state in kind or through arrangement can for referential when study, research can individuality representatively in one species, also can be used as the important references of universal publicity of scientific research, teaching and Sci-tech Knowledge.Scientific value, ornamental value and reserve value that what animal and tissue specimen contained can not be substituted, other artworks of erect image equally enter into family, become the indoor new ornament of people.One makes exquisite sample and not only brings people's knowledge, also brings the enjoyment of people's art simultaneously.Perfect animal muscle sample is a vivid practical teaching aid, can be again a consummate artwork, it represent colleges and universities, a subject development level and grasp the professional skill of this disciplinary study person.Preparation of specimen is also a research work, in manufacturing process, can constantly discover problems and solve them, and creates new technology, can broaden one's knowledge simultaneously, tempers thinking and the manipulative ability of participant.Be made into whole muscle specimen, whole-body muscle structure can be demonstrated, sample structure is attractive in appearance, form knows nature, not only " Zootomy " teaching in as directly perceived, vivid teaching aid, and can allow students' participation in manufacturing process, allow student understand and grasp preparation of specimen's process, simultaneously the starting of training student, beat one's brains ability.Contribute to, to the understanding of student to animal whole-body muscle overview, grasping the contour structures of whole-body muscle, good help is all played to the understanding of the Name & Location relation of muscle, contribute to improving teaching efficiency.
Summary of the invention
The present invention seeks to as solving the problems of the technologies described above, providing the muscle specimen method for making of a kind of adult otter, its preparation method is simple, and prepared sample can demonstrate the whole-body muscle of adult otter, and structure is attractive in appearance, and form knows nature.
A muscle specimen method for making for adult otter, its concrete preparation process comprises:
Step one, solution preparation: the mass percent with distilled water hydrogen peroxide being diluted to hydrogen peroxide is the solution of 3.5%, prepares the hydrogen peroxide after dilution, for subsequent use; Take washing powder, add distilled water, prepare the detergent solution that mass percent is 4%-5%; Prepared solution is wanted matching while using or is placed in 4 DEG C of preservations;
After step 2, selection anesthesia, through the corpse of the adult otter that perfusion fixation is put to death;
Step 3, peeling: faced upward by otter corpse and be put on autopsy table, cut the osculum that diameter is 1-1.5cm respectively with operating scissors inside front wrist joint and hind leg gambrel, then respectively from cutting off land otter fur skin inside cut osculum to anus front; Then be separated land otter fur skin, by the method for drill drift, skin sloughed to neck, become dermatotome housing type outwardly; Cut off the auricular cartilage of ear again, be separated eye and mouth skin of lip, by complete for skin stripping, finally gill;
The whole-body muscle of step 4, separation otter: the muscle isolating otter whole body with scalpel; Concrete operation method is: isolate scalp flesh, musculus cutaneus colli and trunk musculus cutaneus after fascia superficialis under stripping head, neck and body skin.Wipe out manadesma and connective tissue along muscle Germ distribution, isolate the facial muscle of head, masseter and temporalis; At neck portion from cucullaris, omoplate transverse process flesh, longest muscle of neck, arm head flesh, musculus sternocephalicus, splenius, semispinalis and musculus hyoideus.At trunk, after peeling off manadesma, connective tissue and blood vessel along the boundary of muscle, terminal, to be separated on latissimus dorsi, chest muscle, veutro serratus, intercostal muscle, levator muscles of ribs, scalenus, musculus longus colli, omoplate the muscle such as flesh, latissimus dorsi, intercostal muscle, back of the body waist eye muscle, iliocostalis, greater psoas muscle, smaller psoas muscle, iliocostalis ditch, dorsal part serratus, external intercostal muscles, intercostals interni, musculus obliquus externus abdominis, oblique, rectus aabdominis, musculus trasversus abdomins.At forelimb, be separated supraspinous muscle, infraspinous muscle, deltoid muscle, subscapular muscle, musculus teres major, arm flesh, antebrachial fascia tensor muscle, arm triceps, arm flesh, arm biceps, forearm, forward foot in a step flesh; At hind leg, be separated gluteus, musculus glutaeus superficialis, gluteus medius, musculus glutaeus profundus, thigh flesh, stern biceps muscle of thigh, semitendinosus, semimembranosus, tensor fasciae late muscle, quadriceps muscle of thigh, gracilis, adductor, shank and rear foot flesh etc.;
Step 5, degreasing: by isolated otter muscle, rinse well with water, be placed in infuse container, then add the ethanol water that mass percent is 80%, makes ethanol water not have otter muscle completely, and soak two days later, taking-up water is rinsed well; Again put back to infuse container, adding volume ratio is again the absolute ethyl alcohol of 1:2 and the mixed solution of dimethylbenzene, absolute ethyl alcohol and dimethylbenzene mixed solution is made not to have otter muscle completely, change when 1-2 days and once soak the absolute ethyl alcohol and dimethylbenzene mixed solution that volume ratio used is 1:2, soak and take out two days later; Or isolated otter muscle is placed in infuse container, pours gasoline into, airtight, soak and carry out degreasing in 6-7 days.In degreasing operation process, to obturage infuse container with Polypropylence Sheet, prevent solution evaporation, in order to avoid damage operating personnel;
Process after step 6, degreasing: the otter muscle after degreasing is placed on dark ventilation and dries in the air 6-7 days, then be that the detergent solution of 4%-5% soaks 1-2h by the otter muscle mass percent dried well, then use clean water three times;
Step 7, above-mentioned steps six is processed after otter muscle insert the hydrogen peroxide after dilution, soak and take out after 2-5 days, rinse well with clear water, dry;
Step 8, with glue by fixing for muscle on a glass, posture when making muscle keep otter to stand, then puts in specimen jar, namely prepares the whole-body muscle sample of adult otter.
Beneficial effect is:
1, the present invention grows up the muscle specimen method for making of otter, and preparation method is simple, easy, with low cost; Adopt ethanol water, absolute ethyl alcohol and dimethylbenzene or gasoline to carry out ungrease treatment to otter muscle, degreasing is thorough, can prevent from using permanent and preserve, and slowly oozing out of otter muscle grease, pollutes mouldy, makes sample impaired, hinders to use and attractive in appearance.Adopt the mass percent of hydrogen oxide to be that after the dilution of 3.5%, hydrogen peroxide is bleached, effect is better and to otter muscle specimen not damaged.
2, the present invention grows up the muscle specimen method for making of otter, the muscle specimen of prepared otter, is easy to preserve, structural integrity is attractive in appearance, morphologic localization knows nature, and whole-body muscle is all clearly illustrated, each block muscle represents all well and is fixed on the physical slot of otter body.Solve the problem of very difficult clear resolution otter whole-body muscle.Muscle is the emphasis in Teaching of Anatomy, and the muscle in Systematic Anatomy teaching is mainly theoretical, and topologic Students in Teaching does not dissect whole muscle; The present invention preferably resolves this difficult problem.Excite again the learning interest of student, improve teaching efficiency.
Embodiment
A muscle specimen method for making for adult otter, its concrete preparation process comprises:
Step one, solution preparation: the mass percent with distilled water hydrogen peroxide being diluted to hydrogen peroxide is the solution of 3.5%, prepares the hydrogen peroxide after dilution, for subsequent use; Take washing powder, add distilled water, prepare the detergent solution that mass percent is 4%-5%; Prepared solution is wanted matching while using or is placed in 4 DEG C of preservations;
After step 2, selection anesthesia, through the corpse of the adult otter that perfusion fixation is put to death;
Step 3, peeling: faced upward by otter corpse and be put on autopsy table, cut the osculum that diameter is 1-1.5cm respectively with operating scissors inside front wrist joint and hind leg gambrel, then respectively from cutting off land otter fur skin inside cut osculum to anus front; Then be separated land otter fur skin, by the method for drill drift, skin sloughed to neck, become dermatotome housing type outwardly; Cut off the auricular cartilage of ear again, be separated eye and mouth skin of lip, by complete for skin stripping, finally gill;
The whole-body muscle of step 4, separation otter: the muscle isolating otter whole body with scalpel; Concrete operation method is: isolate scalp flesh, musculus cutaneus colli and trunk musculus cutaneus after fascia superficialis under stripping head, neck and body skin.Wipe out manadesma and connective tissue along muscle Germ distribution, isolate the facial muscle of head, masseter and temporalis; At neck portion from cucullaris, omoplate transverse process flesh, longest muscle of neck, arm head flesh, musculus sternocephalicus, splenius, semispinalis and musculus hyoideus.At trunk, after peeling off manadesma, connective tissue and blood vessel along the boundary of muscle, terminal, to be separated on latissimus dorsi, chest muscle, veutro serratus, intercostal muscle, levator muscles of ribs, scalenus, musculus longus colli, omoplate the muscle such as flesh, latissimus dorsi, intercostal muscle, back of the body waist eye muscle, iliocostalis, greater psoas muscle, smaller psoas muscle, iliocostalis ditch, dorsal part serratus, external intercostal muscles, intercostals interni, musculus obliquus externus abdominis, oblique, rectus aabdominis, musculus trasversus abdomins.At forelimb, be separated supraspinous muscle, infraspinous muscle, deltoid muscle, subscapular muscle, musculus teres major, arm flesh, antebrachial fascia tensor muscle, arm triceps, arm flesh, arm biceps, forearm, forward foot in a step flesh; At hind leg, be separated gluteus, musculus glutaeus superficialis, gluteus medius, musculus glutaeus profundus, thigh flesh, stern biceps muscle of thigh, semitendinosus, semimembranosus, tensor fasciae late muscle, quadriceps muscle of thigh, gracilis, adductor, shank and rear foot flesh etc.;
Step 5, degreasing: by isolated otter muscle, rinse well with water, be placed in infuse container, then add the ethanol water that mass percent is 80%, makes ethanol water not have otter muscle completely, and soak two days later, taking-up water is rinsed well; Again put back to infuse container, adding volume ratio is again the absolute ethyl alcohol of 1:2 and the mixed solution of dimethylbenzene, absolute ethyl alcohol and dimethylbenzene mixed solution is made not to have otter muscle completely, change when 1-2 days and once soak the absolute ethyl alcohol and dimethylbenzene mixed solution that volume ratio used is 1:2, soak and take out two days later; Or isolated otter muscle is placed in infuse container, pours gasoline into, airtight, soak and carry out degreasing in about 6-7 days.In degreasing operation process, to obturage infuse container with Polypropylence Sheet, prevent solution evaporation, in order to avoid damage operating personnel;
Process after step 6, degreasing: the otter muscle after degreasing is placed on dark ventilation and dries in the air 6-7 days, then be that the detergent solution of 4%-5% soaks 1-2h by the otter muscle mass percent dried well, then use clean water three times;
Step 7, above-mentioned steps six is processed after otter muscle insert the hydrogen peroxide after dilution, soak and take out after 2-5 days, rinse well with clear water, dry;
Step 8, with glue by fixing for muscle on a glass, posture when making muscle keep otter to stand, then puts in specimen jar, namely prepares the whole-body muscle sample of adult otter.
Following examples can make the present invention of those skilled in the art's complete understanding, but do not limit the present invention in any way.
embodiment 1
A muscle specimen method for making for adult otter, its concrete preparation process comprises:
Step one, the adult otter one selecting build plentiful and substantial, healthy and strong, claim its body weight;
Step 2, solution preparation: the body weight according to otter takes yellow Jackets, standard is that every 1kg otter takes yellow Jackets 45mg, is dissolved in stroke-physiological saline solution and is made into the Nembutal sodium solution that massfraction is 3%, for subsequent use; The mass percent with distilled water formalin solution being diluted to formaldehyde is the solution of 7%, prepares the formalin solution after dilution, for subsequent use; The mass percent with distilled water hydrogen peroxide being diluted to hydrogen peroxide is the solution of 3%, prepares the hydrogen peroxide after dilution, for subsequent use; Take washing powder, add distilled water, prepare the detergent solution that mass percent is 4%; Prepared solution want matching while using or be placed in 4 DEG C for subsequent use;
Step 3, perfusion fixation: draw the Nembutal sodium solution prepared by above-mentioned steps two with syringe, adopt intraperitoneal injection anesthesia otter; Then open at the skin place of otter jugular vein ditch the mouth that diameter is 3cm with scalpel, expose arteria carotis communis; On arteria carotis communis, cut with operating scissors the osculum that diameter is 0.3cm, in osculum, insert perfusion syringe needle, fix with hemostatic forceps, quick filling 3000ml physiological saline, treats that blood is rinsed well, after namely liver bleaches, formalin solution after perfusion 5000ml dilution, first quick and back slow, continues about two hours;
Step 4, peeling: faced upward by otter corpse and be put on autopsy table, cut the osculum that diameter is 1cm respectively with operating scissors inside front wrist joint and hind leg gambrel, then respectively from cutting off land otter fur skin inside cut osculum to anus front; Then be separated land otter fur skin, by the method for drill drift, skin sloughed to neck, become dermatotome housing type outwardly; Cut off the auricular cartilage of ear again, be separated eye and mouth skin of lip, by complete for skin stripping, finally gill;
The whole-body muscle of step 5, separation otter: the muscle isolating otter whole body with scalpel; Concrete operation method is: isolate scalp flesh, musculus cutaneus colli and trunk musculus cutaneus after fascia superficialis under stripping head, neck and body skin.Wipe out manadesma and connective tissue along muscle Germ distribution, isolate the facial muscle of head, masseter and temporalis; At neck portion from cucullaris, omoplate transverse process flesh, longest muscle of neck, arm head flesh, musculus sternocephalicus, splenius, semispinalis and musculus hyoideus.At trunk, after peeling off manadesma, connective tissue and blood vessel along the boundary of muscle, terminal, to be separated on latissimus dorsi, chest muscle, veutro serratus, intercostal muscle, levator muscles of ribs, scalenus, musculus longus colli, omoplate the muscle such as flesh, latissimus dorsi, intercostal muscle, back of the body waist eye muscle, iliocostalis, greater psoas muscle, smaller psoas muscle, iliocostalis ditch, dorsal part serratus, external intercostal muscles, intercostals interni, musculus obliquus externus abdominis, oblique, rectus aabdominis, musculus trasversus abdomins.At forelimb, be separated supraspinous muscle, infraspinous muscle, deltoid muscle, subscapular muscle, musculus teres major, arm flesh, antebrachial fascia tensor muscle, arm triceps, arm flesh, arm biceps, forearm, forward foot in a step flesh; At hind leg, be separated gluteus, musculus glutaeus superficialis, gluteus medius, musculus glutaeus profundus, thigh flesh, stern biceps muscle of thigh, semitendinosus, semimembranosus, tensor fasciae late muscle, quadriceps muscle of thigh, gracilis, adductor, shank and rear foot flesh etc.;
Step 6, degreasing: by isolated otter muscle, rinse well with water, be placed in infuse container, then add the ethanol water that mass percent is 80%, makes ethanol water not have otter muscle completely, and soak two days later, taking-up water is rinsed well; Again put back to infuse container, adding volume ratio is again the absolute ethyl alcohol of 1:2 and the mixed solution of dimethylbenzene, absolute ethyl alcohol and dimethylbenzene mixed solution is made not to have otter muscle completely, changed every 1 day time and once soak the absolute ethyl alcohol and dimethylbenzene mixed solution that volume ratio used is 1:2, soak and take out two days later; In degreasing operation process, to obturage infuse container with Polypropylence Sheet, prevent solution evaporation, in order to avoid damage operating personnel;
Process after step 7, degreasing: the otter muscle after degreasing is placed on dark ventilation and dries in the air 6 days, then by the otter muscle mass percent dried well be 4% detergent solution soak 1-2h, then use clean water three times;
Step 8, above-mentioned steps six is processed after otter muscle insert the hydrogen peroxide after dilution, soak and take out after 2-5 days, rinse well with clear water, dry;
Step 9, with glue by fixing for muscle on a glass, posture when making muscle keep otter to stand, then puts in specimen jar, namely prepares the whole-body muscle sample of adult otter.
embodiment 2
A muscle specimen method for making for adult otter, its concrete preparation process comprises:
Step one, the adult otter one selecting build plentiful and substantial, healthy and strong, claim its body weight;
Step 2, solution preparation: the body weight according to otter takes yellow Jackets, standard is that every 1kg otter takes yellow Jackets 45mg, is dissolved in stroke-physiological saline solution and is made into the Nembutal sodium solution that massfraction is 3%, for subsequent use; The mass percent with distilled water formalin solution being diluted to formaldehyde is the solution of 7%, prepares the formalin solution after dilution, for subsequent use; The mass percent with distilled water hydrogen peroxide being diluted to hydrogen peroxide is the solution of 3%, prepares the hydrogen peroxide after dilution, for subsequent use; Take washing powder, add distilled water, prepare the detergent solution that mass percent is 5%; Prepared solution want matching while using or be placed in 4 DEG C for subsequent use;
Step 3, perfusion fixation: draw the Nembutal sodium solution prepared by above-mentioned steps two with syringe, adopt intraperitoneal injection anesthesia otter; Then open at the skin place of otter jugular vein ditch the mouth that diameter is 3cm with scalpel, expose arteria carotis communis; On arteria carotis communis, cut with operating scissors the osculum that diameter is 0.3cm, in osculum, insert perfusion syringe needle, fix with hemostatic forceps, quick filling 3000ml physiological saline, treats that blood is rinsed well, after namely liver bleaches, formalin solution after perfusion 5000ml dilution, first quick and back slow, continues about two hours;
Step 4, peeling: faced upward by otter corpse and be put on autopsy table, cut the osculum that diameter is 1.5cm respectively with operating scissors inside front wrist joint and hind leg gambrel, then respectively from cutting off land otter fur skin inside cut osculum to anus front; Then be separated land otter fur skin, by the method for drill drift, skin sloughed to neck, become dermatotome housing type outwardly; Cut off the auricular cartilage of ear again, be separated eye and mouth skin of lip, by complete for skin stripping, finally gill;
The whole-body muscle of step 5, separation otter: the muscle isolating otter whole body with scalpel; Concrete operation method is: isolate scalp flesh, musculus cutaneus colli and trunk musculus cutaneus after fascia superficialis under stripping head, neck and body skin.Wipe out manadesma and connective tissue along muscle Germ distribution, isolate the facial muscle of head, masseter and temporalis; At neck portion from cucullaris, omoplate transverse process flesh, longest muscle of neck, arm head flesh, musculus sternocephalicus, splenius, semispinalis and musculus hyoideus.At trunk, after peeling off manadesma, connective tissue and blood vessel along the boundary of muscle, terminal, to be separated on latissimus dorsi, chest muscle, veutro serratus, intercostal muscle, levator muscles of ribs, scalenus, musculus longus colli, omoplate the muscle such as flesh, latissimus dorsi, intercostal muscle, back of the body waist eye muscle, iliocostalis, greater psoas muscle, smaller psoas muscle, iliocostalis ditch, dorsal part serratus, external intercostal muscles, intercostals interni, musculus obliquus externus abdominis, oblique, rectus aabdominis, musculus trasversus abdomins.At forelimb, be separated supraspinous muscle, infraspinous muscle, deltoid muscle, subscapular muscle, musculus teres major, arm flesh, antebrachial fascia tensor muscle, arm triceps, arm flesh, arm biceps, forearm, forward foot in a step flesh; At hind leg, be separated gluteus, musculus glutaeus superficialis, gluteus medius, musculus glutaeus profundus, thigh flesh, stern biceps muscle of thigh, semitendinosus, semimembranosus, tensor fasciae late muscle, quadriceps muscle of thigh, gracilis, adductor, shank and rear foot flesh etc.;
Step 6, degreasing: by isolated otter muscle, rinse well with water, be placed in infuse container, then add the ethanol water that mass percent is 80%, makes ethanol water not have otter muscle completely, and soak two days later, taking-up water is rinsed well; Again put back to infuse container, adding volume ratio is again the absolute ethyl alcohol of 1:2 and the mixed solution of dimethylbenzene, absolute ethyl alcohol and dimethylbenzene mixed solution is made not to have otter muscle completely, changed every 2 days time and once soak the absolute ethyl alcohol and dimethylbenzene mixed solution that volume ratio used is 1:2, soak and take out two days later; In degreasing operation process, to obturage infuse container with Polypropylence Sheet, prevent solution evaporation, in order to avoid damage operating personnel;
Process after step 7, degreasing: the otter muscle after degreasing is placed on dark ventilation and dries in the air 7 days, then by the otter muscle mass percent dried well be 5% detergent solution soak 2h, then use clean water three times;
Step 8, above-mentioned steps six is processed after otter muscle insert the hydrogen peroxide after dilution, soak and take out after 5 days, rinse well with clear water, dry;
Step 9, with glue by fixing for muscle on a glass, posture when making muscle keep otter to stand, then puts in specimen jar, namely prepares the whole-body muscle sample of adult otter.
embodiment 3
A muscle specimen method for making for adult otter, its concrete preparation process comprises:
Step one, the adult otter one selecting build plentiful and substantial, healthy and strong, claim its body weight;
Step 2, solution preparation: the body weight according to otter takes yellow Jackets, standard is that every 1kg otter takes yellow Jackets 45mg, is dissolved in stroke-physiological saline solution and is made into the Nembutal sodium solution that massfraction is 3%, for subsequent use; The mass percent with distilled water formalin solution being diluted to formaldehyde is the solution of 7%, prepares the formalin solution after dilution, for subsequent use; The mass percent with distilled water hydrogen peroxide being diluted to hydrogen peroxide is the solution of 3%, prepares the hydrogen peroxide after dilution, for subsequent use; Take washing powder, add distilled water, prepare the detergent solution that mass percent is 4%; Prepared solution want matching while using or be placed in 4 DEG C for subsequent use;
Step 3, perfusion fixation: draw the Nembutal sodium solution prepared by above-mentioned steps two with syringe, adopt intraperitoneal injection anesthesia otter; Then open at the skin place of otter jugular vein ditch the mouth that diameter is 3cm with scalpel, expose arteria carotis communis; On arteria carotis communis, cut with operating scissors the osculum that diameter is 0.3cm, in osculum, insert perfusion syringe needle, fix with hemostatic forceps, quick filling 3000ml physiological saline, treats that blood is rinsed well, after namely liver bleaches, formalin solution after perfusion 5000ml dilution, first quick and back slow, continues about two hours;
Step 4, peeling: faced upward by otter corpse and be put on autopsy table, cut the osculum that diameter is 1.2cm respectively with operating scissors inside front wrist joint and hind leg gambrel, then respectively from cutting off land otter fur skin inside cut osculum to anus front; Then be separated land otter fur skin, by the method for drill drift, skin sloughed to neck, become dermatotome housing type outwardly; Cut off the auricular cartilage of ear again, be separated eye and mouth skin of lip, by complete for skin stripping, finally gill;
The whole-body muscle of step 5, separation otter: the muscle isolating otter whole body with scalpel; Concrete operation method is: isolate scalp flesh, musculus cutaneus colli and trunk musculus cutaneus after fascia superficialis under stripping head, neck and body skin.Wipe out manadesma and connective tissue along muscle Germ distribution, isolate the facial muscle of head, masseter and temporalis; At neck portion from cucullaris, omoplate transverse process flesh, longest muscle of neck, arm head flesh, musculus sternocephalicus, splenius, semispinalis and musculus hyoideus.At trunk, after peeling off manadesma, connective tissue and blood vessel along the boundary of muscle, terminal, to be separated on latissimus dorsi, chest muscle, veutro serratus, intercostal muscle, levator muscles of ribs, scalenus, musculus longus colli, omoplate the muscle such as flesh, latissimus dorsi, intercostal muscle, back of the body waist eye muscle, iliocostalis, greater psoas muscle, smaller psoas muscle, iliocostalis ditch, dorsal part serratus, external intercostal muscles, intercostals interni, musculus obliquus externus abdominis, oblique, rectus aabdominis, musculus trasversus abdomins.At forelimb, be separated supraspinous muscle, infraspinous muscle, deltoid muscle, subscapular muscle, musculus teres major, arm flesh, antebrachial fascia tensor muscle, arm triceps, arm flesh, arm biceps, forearm, forward foot in a step flesh; At hind leg, be separated gluteus, musculus glutaeus superficialis, gluteus medius, musculus glutaeus profundus, thigh flesh, stern biceps muscle of thigh, semitendinosus, semimembranosus, tensor fasciae late muscle, quadriceps muscle of thigh, gracilis, adductor, shank and rear foot flesh etc.;
Step 6, degreasing: by isolated otter muscle, rinse well with water, be placed in infuse container, then add the ethanol water that mass percent is 80%, makes ethanol water not have otter muscle completely, and soak two days later, taking-up water is rinsed well; Again put back to infuse container, adding volume ratio is again the absolute ethyl alcohol of 1:2 and the mixed solution of dimethylbenzene, absolute ethyl alcohol and dimethylbenzene mixed solution is made not to have otter muscle completely, changed every 2 days time and once soak the absolute ethyl alcohol and dimethylbenzene mixed solution that volume ratio used is 1:2, soak and take out two days later; In degreasing operation process, to obturage infuse container with Polypropylence Sheet, prevent solution evaporation, in order to avoid damage operating personnel;
Process after step 7, degreasing: the otter muscle after degreasing is placed on dark ventilation and dries in the air 7 days, then by the otter muscle mass percent dried well be 4.5% detergent solution soak 1.5h, then use clean water three times;
Step 8, above-mentioned steps six is processed after otter muscle insert the hydrogen peroxide after dilution, soak and take out after 4 days, rinse well with clear water, dry;
Step 9, with glue by fixing for muscle on a glass, posture when making muscle keep otter to stand, then puts in specimen jar, namely prepares the whole-body muscle sample of adult otter.
embodiment 4
A muscle specimen method for making for adult otter, its concrete preparation process comprises:
Step one, the adult otter one selecting build plentiful and substantial, healthy and strong, claim its body weight;
Step 2, solution preparation: the body weight according to otter takes yellow Jackets, standard is that every 1kg otter takes yellow Jackets 45mg, is dissolved in stroke-physiological saline solution and is made into the Nembutal sodium solution that massfraction is 3%, for subsequent use; The mass percent with distilled water formalin solution being diluted to formaldehyde is the solution of 7%, prepares the formalin solution after dilution, for subsequent use; The mass percent with distilled water hydrogen peroxide being diluted to hydrogen peroxide is the solution of 3%, prepares the hydrogen peroxide after dilution, for subsequent use; Take washing powder, add distilled water, prepare the detergent solution that mass percent is 3%-5%; Prepared solution want matching while using or be placed in 4 DEG C for subsequent use;
Step 3, perfusion fixation: draw the Nembutal sodium solution prepared by above-mentioned steps two with syringe, adopt intraperitoneal injection anesthesia otter; Then open at the skin place of otter jugular vein ditch the mouth that diameter is 3cm with scalpel, expose arteria carotis communis; On arteria carotis communis, cut with operating scissors the osculum that diameter is 0.3cm, in osculum, insert perfusion syringe needle, fix with hemostatic forceps, quick filling 3000ml physiological saline, treats that blood is rinsed well, after namely liver bleaches, formalin solution after perfusion 5000ml dilution, first quick and back slow, continues about two hours;
Step 4, peeling: faced upward by otter corpse and be put on autopsy table, cut the osculum that diameter is 1.2cm respectively with operating scissors inside front wrist joint and hind leg gambrel, then respectively from cutting off land otter fur skin inside cut osculum to anus front; Then be separated land otter fur skin, by the method for drill drift, skin sloughed to neck, become dermatotome housing type outwardly; Cut off the auricular cartilage of ear again, be separated eye and mouth skin of lip, by complete for skin stripping, finally gill;
The whole-body muscle of step 5, separation otter: the muscle isolating otter whole body with scalpel; Concrete operation method is: isolate scalp flesh, musculus cutaneus colli and trunk musculus cutaneus after fascia superficialis under stripping head, neck and body skin.Wipe out manadesma and connective tissue along muscle Germ distribution, isolate the facial muscle of head, masseter and temporalis; At neck portion from cucullaris, omoplate transverse process flesh, longest muscle of neck, arm head flesh, musculus sternocephalicus, splenius, semispinalis and musculus hyoideus.At trunk, after peeling off manadesma, connective tissue and blood vessel along the boundary of muscle, terminal, to be separated on latissimus dorsi, chest muscle, veutro serratus, intercostal muscle, levator muscles of ribs, scalenus, musculus longus colli, omoplate the muscle such as flesh, latissimus dorsi, intercostal muscle, back of the body waist eye muscle, iliocostalis, greater psoas muscle, smaller psoas muscle, iliocostalis ditch, dorsal part serratus, external intercostal muscles, intercostals interni, musculus obliquus externus abdominis, oblique, rectus aabdominis, musculus trasversus abdomins.At forelimb, be separated supraspinous muscle, infraspinous muscle, deltoid muscle, subscapular muscle, musculus teres major, arm flesh, antebrachial fascia tensor muscle, arm triceps, arm flesh, arm biceps, forearm, forward foot in a step flesh; At hind leg, be separated gluteus, musculus glutaeus superficialis, gluteus medius, musculus glutaeus profundus, thigh flesh, stern biceps muscle of thigh, semitendinosus, semimembranosus, tensor fasciae late muscle, quadriceps muscle of thigh, gracilis, adductor, shank and rear foot flesh etc.;
Step 6, degreasing: by isolated otter muscle, rinse well with water, be placed in infuse container, pour gasoline into, airtight, soak and carry out degreasing in about 7 days.In degreasing operation process, to obturage infuse container with Polypropylence Sheet, prevent solution evaporation, in order to avoid damage operating personnel;
Process after step 7, degreasing: the otter muscle after degreasing is placed on dark ventilation and dries in the air 7 days, then by the otter muscle mass percent dried well be 5% detergent solution soak 2h, then use clean water three times;
Step 8, above-mentioned steps six is processed after otter muscle insert the hydrogen peroxide after dilution, soak and take out after 3 days, rinse well with clear water, dry;
Step 9, with glue by fixing for muscle on a glass, posture when making muscle keep otter to stand, then puts in specimen jar, namely prepares the whole-body muscle sample of adult otter.
Claims (2)
1. a whole-body muscle Slide processing for adult otter, is characterized in that: its concrete preparation process comprises:
Step one, solution preparation: the mass percent with distilled water hydrogen peroxide being diluted to hydrogen peroxide is the solution of 3.5%, prepares the hydrogen peroxide after dilution, for subsequent use; Take washing powder, add distilled water, prepare the detergent solution that mass percent is 4%-5%; Prepared solution is wanted matching while using or is placed in 4 DEG C of preservations;
The corpse of step 2, the adult otter of selection;
Step 3, peeling: faced upward by otter corpse and be put on autopsy table, cut the osculum that diameter is 1-1.5cm respectively with operating scissors inside front wrist joint and hind leg gambrel, then respectively from cutting off land otter fur skin inside cut osculum to anus front; Then be separated land otter fur skin, by the method for drill drift, skin sloughed to neck, become dermatotome housing type outwardly; Cut off the auricular cartilage of ear again, be separated eye and mouth skin of lip, by complete for skin stripping, finally gill;
The whole-body muscle of step 4, separation otter: the muscle isolating otter whole body with scalpel;
Step 5, degreasing: by isolated otter muscle, rinse well with water, be placed in infuse container, then add the ethanol water that mass percent is 80%, makes ethanol water not have otter muscle completely, and soak two days later, taking-up water is rinsed well; Again put back to infuse container, adding volume ratio is again the absolute ethyl alcohol of 1:2 and the mixed solution of dimethylbenzene, absolute ethyl alcohol and dimethylbenzene mixed solution is made not to have otter muscle completely, change when 1-2 days and once soak the absolute ethyl alcohol and dimethylbenzene mixed solution that volume ratio used is 1:2, soak and take out two days later; In the operating process of described step 5 degreasing, to obturage infuse container with Polypropylence Sheet, prevent solution evaporation;
Process after step 6, degreasing: the otter muscle after degreasing is placed on dark ventilation and dries in the air 6-7 days, then be that the detergent solution of 4%-5% soaks 1-2h by the otter muscle mass percent dried well, then use clean water three times;
Step 7, above-mentioned steps six is processed after otter muscle insert the hydrogen peroxide after dilution, soak and take out after 2-5 days, rinse well with clear water, dry;
Step 8, with glue by fixing for muscle on a glass, posture when making muscle keep otter to stand, then puts in specimen jar, namely prepares the whole-body muscle sample of adult otter.
2. the whole-body muscle Slide processing of a kind of adult otter as claimed in claim 1, it is characterized in that: the degreasing method in described step 5 adopts in an alternative manner: isolated otter muscle specimen is placed in infuse container, pour gasoline into, airtight, soak and carry out degreasing in 6-7 days.
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