CN107767750A - A kind of operating theater instruments biological sample and preparation method thereof - Google Patents

A kind of operating theater instruments biological sample and preparation method thereof Download PDF

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Publication number
CN107767750A
CN107767750A CN201711245287.4A CN201711245287A CN107767750A CN 107767750 A CN107767750 A CN 107767750A CN 201711245287 A CN201711245287 A CN 201711245287A CN 107767750 A CN107767750 A CN 107767750A
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human body
fracture
body specimen
biological sample
operating theater
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Inventor
程明亮
赵顺
贠跃进
邢现锋
刘会民
吉晓磊
王永超
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HENAN ZHONGBO BIO-PLASTINATION TECHNIQUE Co Ltd
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HENAN ZHONGBO BIO-PLASTINATION TECHNIQUE Co Ltd
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Priority to CN201711245287.4A priority Critical patent/CN107767750A/en
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    • GPHYSICS
    • G09EDUCATION; CRYPTOGRAPHY; DISPLAY; ADVERTISING; SEALS
    • G09BEDUCATIONAL OR DEMONSTRATION APPLIANCES; APPLIANCES FOR TEACHING, OR COMMUNICATING WITH, THE BLIND, DEAF OR MUTE; MODELS; PLANETARIA; GLOBES; MAPS; DIAGRAMS
    • G09B23/00Models for scientific, medical, or mathematical purposes, e.g. full-sized devices for demonstration purposes
    • G09B23/28Models for scientific, medical, or mathematical purposes, e.g. full-sized devices for demonstration purposes for medicine
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00Preservation of bodies of humans or animals, or parts thereof

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  • Life Sciences & Earth Sciences (AREA)
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  • Business, Economics & Management (AREA)
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  • Medical Informatics (AREA)
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  • Educational Technology (AREA)
  • Theoretical Computer Science (AREA)
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  • Wood Science & Technology (AREA)
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Abstract

The present invention relates to a kind of operating theater instruments biological sample and preparation method thereof, operating theater instruments biological sample includes human body specimen, the artery of the human body specimen silica gel pigment different with color is perfused with vein, extruding is injected with the liquid silica gel for replacing moisture and fat in remaining tissue, and fracture apparatus is provided with bone.Using real biology as sample, sample general shape strong sense of reality, is advantageous to the observation of later stage sample, and use is simple to operate, clearly shows that the position of apparatus, and the anatomical structure of surrounding, facilitates teachers ' teaching and Students ' Learning without change.

Description

A kind of operating theater instruments biological sample and preparation method thereof
Technical field
The invention belongs to biological plasticized field, more particularly to a kind of operating theater instruments biological sample and preparation method thereof.
Background technology
After the entirety of animal or plant or topical finish, by processing, its original shape or feature are kept, and be stored in scientific research In the laboratory of unit or school, for the course teachings such as biology, scientific research or the material object viewed and emulated is displayed, is referred to as biology mark This.Common biological sample is mainly humid preparation, although the Saving specimen time length of this form, because humid preparation needs Ready-made sample body is stored in a container, user can not directly contact sample body, so the biological sample exists During for teaching and experiment, the problem of interactive experience is not strong often occurs, causes user can not intuitively experience it to life The operation of thing sample.
The content of the invention
The present invention is intended to provide a kind of operating theater instruments biological sample of strong sense of reality, while additionally provide and be exclusively used in above-mentioned hand The preparation method of art apparatus biological sample.
In order to solve the above technical problems, the technical scheme of operating theater instruments biological sample is as follows in the present invention:
Operating theater instruments biological sample, including human body specimen, the artery of human body specimen with to be perfused with color in vein different Silica gel pigment, in remaining tissue extruding be injected with the liquid silica gel for replacing moisture and fat, fracture device is installed on bone Tool.
The color of silica gel pigment is red in artery, and the color of silica gel pigment is blueness in vein.
Bone is provided with the fracture structure being located at at least one in skull, upper limbs bone, clavicle and backbone on position, described Fracture apparatus is the fracture fixation part being connected in the fracture structure.
Bone includes hip joint, and the femoral head prosthesis, plastic gasket and acetabular bone that the fracture apparatus includes fastening successively are false Body, femoral head prosthesis are connected to femur end, and acetabular component is connected on hipbone.
Bone includes knee joint, and the fracture apparatus includes knee-joint prosthesis, and one end of knee-joint prosthesis is connected to femur Lower end, the other end are connected to proximal ends of tibia.
The otch positioned at fracture apparatus installation site is offered in musculature, fracture apparatus is exposed in otch.
The technical scheme of operating theater instruments biological sample preparation method is as follows in the present invention:
Operating theater instruments biological sample preparation method, comprises the following steps:
A, selection:Choose human body specimen;
B, preservative treatment:Human body specimen is irrigated with 15%-20% formalin, always moved by femoral artery and neck Arteries and veins penetrates into formalin at each position of human body specimen, and in artery and venous perfusion silica gel pigment, the red dye of arterial perfusion Material, venous perfusion indigo plant dyestuff, be then put into 10% formalin-fixed liquid soak 3 months it is stand-by;
C, dissect:Human body specimen in upper step is made according to anatomical requirements, skin median sagittal is cut, and peels off the shallow muscle of skin Film, left side show muscle and deep nervus vasculairs, and right side shows superficial muscular nervus vasculairs;
D, drift:The human body specimen dissected is placed in 10% hydrogen peroxide and drifted 2-3 days, until the tissue of human body specimen Color bleaches;
E, dewatering and defatting:The human body specimen bleached is put into acetone, kept for 80-90 days, until human body specimen reclaimed water Divide and fat displacement is complete;
F, vacuum impregnation:Human body specimen in upper step is kept for 60-70 days in vacuum pressure storehouse, by extruding liquid silica gel Displace the acetone in the tissue of human body specimen;
G, shape:After the human body specimen that dipping is completed is taken out from liquid silica gel, shape is set with reinforcing bar;
Apparatus is installed h,;The musculature of human body specimen is cut, is installed in the fracture of human body specimen bone setting Fracture apparatus.
After h steps, also have:
I, repair:The musculature of human body specimen is repaired smooth;
J, solidify:The human body specimen that upper step obtains is carried out solidification 10-15 days with curing agent;
K, repair:After solidification, silica gel unnecessary on human body specimen and the musculature of out-of-flatness are repaired clean, neat;
L, reset:Free nervus vasculairs are resetted on origin-location, that is, complete the making of sample.
In a steps, human body specimen is selected without fracture, without lesion.
The beneficial effects of the invention are as follows:
Sample is used as using real biology, sample general shape is without change, strong sense of reality, is advantageous to later stage sample and observes, And use is simple to operate, clearly shows that the position of apparatus, and the anatomical structure of surrounding, teachers ' teaching and student is facilitated to learn Practise.
Brief description of the drawings
Fig. 1 is the knee schematic diagram of biological sample;
Fig. 2 is the hand schematic diagram of biological sample;
Fig. 3 is the arm schematic diagram of biological sample;
Fig. 4 is the shoulder schematic diagram of biological sample.
Embodiment
Describe the present invention in detail below with reference to accompanying drawing and in conjunction with the embodiments.It should be noted that do not conflicting In the case of, the feature in embodiment and embodiment in the present invention can be mutually combined.
The embodiment of the operating theater instruments biological sample preparation method of the present invention:This method comprises the following steps:
1. selection:It is complete from skin, no wound, no fracture, the human body specimen of no lesion.
2. preservative treatment:Sample is irrigated with 15%-20% formalin, by big blood vessel, (femoral artery, neck are total Artery) formalin is penetrated into each position of sample, action venous perfusion silica gel pigment of going forward side by side, arterial perfusion red, Venous perfusion indigo plant dyestuff, be finally put into 10% formalin-fixed liquid soak 3 months it is stand-by.
3. dissection:Sample is made according to anatomical requirements, skin median sagittal is cut, and peels off skin fascia superficialis, right side exists Shallow-layer vein is found out under fascia superficialis, artery, nerve, deep fascia is then removed and shows musculature, left side is in layer muscle vascular god Through finding out shallow artery under fascia superficialis, nerve, remove deep fascia and show musculature, it is deep then to remove part superficial muscular display Layer artery, vein, neural such as upper limbs remove the bicipital muscle of arm, musculus palmaris longus.Lower limb remove sartorius, rectus femoris, gluteus maximus, sura Flesh etc..
4. drift:Drifted 2-3 days having dissected sample and being placed in 10% hydrogen peroxide, until sample tissue color bleaches i.e. Can.
5. dewatering and defatting:The biological sample bleached was put into acetone by 80-90 days, moisture and fat displacement is dry Only.
6. vacuum impregnation:Pass through 60-70 days in vacuum pressure storehouse, displaced by extruding liquid silica gel in sample tissue Acetone.
7. sizing:The sample that dipping is completed is taken out from silica gel, as needed, sample is set shape, root with reinforcing bar According to the conventional position of operation, positioned on sample.
8. apparatus is installed;1) instruments:A. internal fixation steel plate for department of orthopedics is a set of, and the artificial knees of 3 .c. of b. external fixator for orthopedics dep close One, section, d. artificial hip joints one, e. skull locks one, f. titanium nets one.2) making steps:Right side on whole plasticizing sample The position fractured according to orthopaedics, first positioning is put, including effracture, upper limb fracture, fracture of clavicle, fracture of lower limb, spine fracture. Replacement operation of artificial femoral head, knee replacements.It is positioned to postpone and from the superficial to the deep opens institutional framework according to sample level, in fracture phase Answer position that apparatus is installed.
9. repair:Musculature is repaired smooth.
10. solidification:Biological sample is made solidification 10-15 days is carried out with curing agent.
11. finishing:After solidification, silica gel unnecessary behind sample and the musculature of out-of-flatness are repaired clean, neat.
12. reset:Free nervus vasculairs are resetted on origin-location, that is, complete the making of sample.
Compared with prior art, the sample of the present embodiment is used as sample using real biology, and sample general shape is without changing Become, strong sense of reality, be advantageous to the observation of later stage sample, and use is simple to operate, clearly shows that the position of apparatus, and surrounding Anatomical structure, facilitate teachers ' teaching and Students ' Learning.Reference frame is provided for clinically surgical operation, 2) the nontoxic nothing of sample Taste, it is easy to preserve for a long time, is used for multiple times and saves instruction cost for school, health can be provided as in air for user for a long time Product.3) it is on plasticizing sample that apparatus people, which plastifies sample, is combined using orthopedic instrument with clinic, according to the height of sample Supporting apparatus is customized, orthopedic instrument is installed in corresponding site.It so can intuitively show position and the week of fracture fixation Enclose the distribution of nerve and blood vessel, the morphosis of muscle.
The embodiment of the operating theater instruments biological sample of the present invention:As shown in Figures 1 to 4, the biological sample is using real Based on human body specimen, in the artery of the human body specimen silica gel pigment different with color is perfused with vein, remaining tissue Extruding is injected with the liquid silica gel for replacing moisture and fat, and fracture apparatus is provided with bone.
Specifically, the color of silica gel pigment is red in artery, and the color of silica gel pigment is blueness in vein.
Specifically, bone includes skull, upper limbs bone 11, clavicle 21, backbone and hand bone 31, wherein
Skull is provided with the effracture structure of simulation effracture line, and being connected with effracture in effracture structure consolidates Determine part, the effracture fixture is exactly the fracture apparatus on skull, it is preferred that effracture fixture selects skull lock.
Upper limbs bone 11 is provided with the upper limb fracture structure of simulation closed fracture of upper limb and open fracture, upper limb fracture Upper limb fracture fixture 12 is connected with structure, upper limb fracture fixture 12 is the fracture apparatus on upper limbs, it is preferred that arm bone Folding fixture 12 selects bone fracture fixation plate.
Clavicle 21 is provided with the fracture of clavicle structure of simulation closed fracture of clavicle and open fracture, fracture of clavicle structure On be connected with fracture of clavicle fixture 22, fracture of clavicle fixture 22 is the fracture apparatus on clavicle 21, it is preferred that fracture of clavicle Fixture 22 selects clavicle fracture fixing plate.
Backbone is provided with the spine fracture structure of simulation spinal bone broken line.Spine fracture is connected with spine fracture structure to consolidate Determine part, spine fracture fixture is epispinal fracture apparatus, it is preferred that spine fracture fixture selects spinal fixation plate.
The finger part of hand bone 31 is provided with care of fractured fingers structure, is connected with care of fractured fingers structure exposed to musculature Outer care of fractured fingers fixture 32, care of fractured fingers fixture 32 are the fracture apparatus on hand bone 31, it is preferred that care of fractured fingers is fixed Part 32 selects finger fixed mount.
Specifically, bone also includes knee joint and hip joint, wherein
Knee joint includes knee-joint prosthesis 41 (i.e. artificial knee joint), and one end of knee-joint prosthesis 41 is connected under femur End, the other end are connected to proximal ends of tibia.
Hip joint includes hip prosthesis (i.e. artificial hip joint), and hip prosthesis is by the femur that interlocks successively from bottom to top Head prosthesis, plastic gasket and acetabular component composition, femoral head prosthesis are connected to femur end, and acetabular component is connected on hipbone.
Specifically, it is further opened with exposing the otch of bone positioned at incision musculature on human body specimen, otch position It is exposed in otch in fracture apparatus installed position, fracture apparatus.
Compared with prior art, the sample of the present embodiment is used as sample using real biology, and sample general shape is without changing Become, strong sense of reality, be advantageous to the observation of later stage sample, and use is simple to operate, clearly shows that the position of apparatus, and surrounding Anatomical structure, facilitate teachers ' teaching and Students ' Learning.Reference frame is provided for clinically surgical operation, 2) the nontoxic nothing of sample Taste, it is easy to preserve for a long time, is used for multiple times and saves instruction cost for school, health can be provided as in air for user for a long time Product.3) it is on plasticizing sample that apparatus people, which plastifies sample, is combined using orthopedic instrument with clinic, according to the height of sample Supporting apparatus is customized, orthopedic instrument is installed in corresponding site.It so can intuitively show position and the week of fracture fixation Enclose the distribution of nerve and blood vessel, the morphosis of muscle.
Finally it should be noted that:The above embodiments are merely illustrative of the technical solutions of the present invention, rather than its limitations;Although The present invention is described in detail with reference to the foregoing embodiments, it will be understood by those within the art that:It still may be used To be modified to the technical scheme described in foregoing embodiments, or equivalent substitution is carried out to which part technical characteristic; And these modification or replace, do not make appropriate technical solution essence depart from various embodiments of the present invention technical scheme spirit and Scope.

Claims (9)

1. operating theater instruments biological sample, including human body specimen, it is characterised in that be perfused with face in the artery and vein of human body specimen The different silica gel pigment of color, in remaining tissue extruding be injected with the liquid silica gel for replacing moisture and fat, installed on bone There is fracture apparatus.
2. operating theater instruments biological sample according to claim 1, it is characterised in that the color of silica gel pigment is red in artery Color, the color of silica gel pigment is blueness in vein.
3. operating theater instruments biological sample according to claim 1, it is characterised in that bone is provided with positioned at skull, upper limbs Fracture structure at least one in bone, clavicle and backbone on position, the fracture apparatus are static connection in the fracture structure On fracture fixation part.
4. operating theater instruments biological sample according to claim 1, it is characterised in that bone includes hip joint, the fracture Apparatus includes femoral head prosthesis, plastic gasket and the acetabular component fastened successively, and femoral head prosthesis static connection is in femur end, hip Mortar prosthese static connection is on hipbone.
5. operating theater instruments biological sample according to claim 1, it is characterised in that bone includes knee joint, the fracture Apparatus includes knee-joint prosthesis, and one end static connection of knee-joint prosthesis is in distal part of femur, and other end static connection is in proximal ends of tibia.
6. operating theater instruments biological sample as claimed in any of claims 1 to 5, it is characterised in that in musculature The otch positioned at fracture apparatus installation site is offered, fracture apparatus is exposed in otch.
7. operating theater instruments biological sample preparation method, it is characterised in that comprise the following steps:
A, selection:Choose human body specimen;
B, preservative treatment:Human body specimen is irrigated with 15%-20% formalin, will by femoral artery and arteria carotis communis Formalin penetrates into each position of human body specimen, and quiet in artery and venous perfusion silica gel pigment, arterial perfusion red Arteries and veins irrigates blue dyestuff, be then put into 10% formalin-fixed liquid soak 3 months it is stand-by;
C, dissect:Human body specimen in upper step is made according to anatomical requirements, skin median sagittal is cut, and peels off skin fascia superficialis, Left side shows muscle and deep nervus vasculairs, and right side shows superficial muscular nervus vasculairs;
D, drift:The human body specimen dissected is placed in 10% hydrogen peroxide and drifted 2-3 days, until the tissue color of human body specimen Bleach;
E, dewatering and defatting:The human body specimen bleached is put into acetone, keep 80-90 days, until human body specimen in moisture and Fat displacement is complete;
F, vacuum impregnation:Human body specimen in upper step is kept for 60-70 days in vacuum pressure storehouse, replaced by extruding liquid silica gel The acetone gone out in the tissue of human body specimen;
G, shape:After the human body specimen that dipping is completed is taken out from liquid silica gel, shape is set with reinforcing bar;
Apparatus is installed h,;The musculature of human body specimen is cut, fracture is installed in the fracture of human body specimen bone setting Apparatus.
8. operating theater instruments biological sample preparation method according to claim 7, it is characterised in that after h steps, also Have:
I, repair:The musculature of human body specimen is repaired smooth;
J, solidify:The human body specimen that upper step obtains is carried out solidification 10-15 days with curing agent;
K, repair:After solidification, silica gel unnecessary on human body specimen and the musculature of out-of-flatness are repaired clean, neat;
L, reset:Free nervus vasculairs are resetted on origin-location, that is, complete the making of sample.
9. the operating theater instruments biological sample preparation method according to claim 7 or 8, it is characterised in that in a steps, human body Sample is selected without fracture, without lesion.
CN201711245287.4A 2017-12-01 2017-12-01 A kind of operating theater instruments biological sample and preparation method thereof Pending CN107767750A (en)

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Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108711352A (en) * 2018-07-27 2018-10-26 大连鸿峰文化发展有限公司 The production method of fur sample
CN108922356A (en) * 2018-07-13 2018-11-30 河南中博科技有限公司 Human body is plasticized nursing teaching sample and preparation method thereof
CN109744225A (en) * 2019-01-17 2019-05-14 河南中博科技有限公司 Animal skeleton is plasticized sample and preparation method thereof
CN110992804A (en) * 2019-12-05 2020-04-10 河南中博科技有限公司 3D printing method for physical specimen
CN112164295A (en) * 2020-10-21 2021-01-01 河南中博科技有限公司 Preparation method of specimen for human cerebrovascular interventional operation
CN112419856A (en) * 2020-11-30 2021-02-26 河南中博生物塑化科技有限公司 Preparation method of specimen for cardiac interventional operation
CN113628517A (en) * 2021-09-08 2021-11-09 河南中博科技有限公司 Method for manufacturing head level anatomy three-dimensional scanning specimen

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CN2641745Y (en) * 2003-05-29 2004-09-15 林梓凌 Nature imitation radius and ulna double-frature clinic diagnosis and treatment experiment model
CN102629430A (en) * 2012-03-28 2012-08-08 上海中医药大学 Manufacturing and identification method of meridian acupoint level anatomy teaching and research display specimen
CN105224311A (en) * 2015-09-07 2016-01-06 郭中献 The method of a kind of human-body biological plasticizing preparation of specimen anatomic teaching software
CN106175903A (en) * 2016-06-29 2016-12-07 浙江工业大学 For repairing the three-dimensional fixed plate manufacture method of fracture mandibular bone and three-dimensional fixed plate
CN106373475A (en) * 2016-11-30 2017-02-01 李懿 Manufacturing method of biological plasticized specimen capable of being assembled

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN2641745Y (en) * 2003-05-29 2004-09-15 林梓凌 Nature imitation radius and ulna double-frature clinic diagnosis and treatment experiment model
CN102629430A (en) * 2012-03-28 2012-08-08 上海中医药大学 Manufacturing and identification method of meridian acupoint level anatomy teaching and research display specimen
CN105224311A (en) * 2015-09-07 2016-01-06 郭中献 The method of a kind of human-body biological plasticizing preparation of specimen anatomic teaching software
CN106175903A (en) * 2016-06-29 2016-12-07 浙江工业大学 For repairing the three-dimensional fixed plate manufacture method of fracture mandibular bone and three-dimensional fixed plate
CN106373475A (en) * 2016-11-30 2017-02-01 李懿 Manufacturing method of biological plasticized specimen capable of being assembled

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108922356A (en) * 2018-07-13 2018-11-30 河南中博科技有限公司 Human body is plasticized nursing teaching sample and preparation method thereof
CN108711352A (en) * 2018-07-27 2018-10-26 大连鸿峰文化发展有限公司 The production method of fur sample
CN109744225A (en) * 2019-01-17 2019-05-14 河南中博科技有限公司 Animal skeleton is plasticized sample and preparation method thereof
CN110992804A (en) * 2019-12-05 2020-04-10 河南中博科技有限公司 3D printing method for physical specimen
CN112164295A (en) * 2020-10-21 2021-01-01 河南中博科技有限公司 Preparation method of specimen for human cerebrovascular interventional operation
CN112419856A (en) * 2020-11-30 2021-02-26 河南中博生物塑化科技有限公司 Preparation method of specimen for cardiac interventional operation
CN113628517A (en) * 2021-09-08 2021-11-09 河南中博科技有限公司 Method for manufacturing head level anatomy three-dimensional scanning specimen

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Application publication date: 20180306