CN106327984B - A kind of production method for bovine jugular vein injection model of growing up - Google Patents
A kind of production method for bovine jugular vein injection model of growing up Download PDFInfo
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Abstract
The present invention relates to a kind of production methods of bovine jugular vein injection model of growing up, first preparation release agent, gypsum cream and silicon rubber；Select Adult Bovine corpse, neck peeling；Jugular vein, arteria carotis and sympathetic trunk are separated, cleans out, support tube is respectively inserted into jugular vein and arteria carotis, then successive brushing release agent and silicon rubber outside the jugular vein and arteria carotis and sympathetic trunk for being inserted with support tube, are made silicone rubber model；In the successive brushing release agent of ox head neck and gypsum cream, ox head neck mold is made；By in the jugular vein ditch of silicone rubber model insertion ox head neck mold, outside is uniformly stained with ox hair, so that ox head neck is kept the posture stood firm with raised head, bovine jugular vein injection model is made.The bovine jugular vein injection model function admirable that the present invention makes, structural integrity is beautiful, and morphologic localization, which understands naturally, can be used for repeatability, to be practiced, and makes up the deficiency that injection operation cannot be repeated several times in the injection of cattle on the hoof jugular vein.
Description
Technical field
The present invention relates to a kind of production method of animal model, specifically a kind of adult bovine jugular vein injection model
Production method.
Background technique
The method that the big animal injection such as ox, horse, donkey mostly uses jugular vein to inject with infusion, still, beginner is in study
Majority cannot smoothly complete operation, lack practices due to focusing on theoretical, not very accurate to the grasp of injection position, often
Animal neck hemotoncus is caused, moreover, more people repeatedly cannot operate repeatedly simultaneously.Production adult bovine jugular vein injection model not only exists
Teaching aid in " zootomy histology " teaching as intuitive image, and student can be allowed to participate in the production process, make to learn
It is raw to understand modelling process, and grasp this technology, while the starting of training student, beating one's brains and operational capacity.But how
Making adult bovine jugular vein injection model handsome in appearance, with excellent quality, that materials are safe is the critical issue that scientific research personnel faces.
Currently, how stereotyped the production of animal tissue's model is, made with traditional agent prescription and manufacturing process
Bovine jugular vein injection model is not able to satisfy various requirements such as safe, vivid, environmentally friendly, high-quality, for example, by using conventional formulation
The gypsum cream of preparation, hardness high-ductility is poor, and Water-saving effect is undesirable, places easily crack for a long time, influence the appearance of model
Quality；When preparing silicon rubber, the accurate proportion disunity of crosslinking agent and curing agent is added, curing time cannot be same with tensile strength
When take into account；Mostly using formaldehyde or similar solution to reach anti-corrosion purpose when conventional model makes, such reagent volatility is extremely strong,
With strong pungent smell, human organ can be damaged, Long Term Contact is also possible to lead to cancer, seriously endangers human body
Health.
Jugular vein is located in jugular vein ditch, and jugular vein ditch, which is located at neck veutro portion, has neck between arm head flesh and musculus sternocephalicus in ditch
Vein, arteria carotis and fan walk sympathetic trunk, wherein jugular vein and arteria carotis are separated by omohyoid, and arteria carotis is walked with fan
Sympathetic trunk is wrapped in same vagina vasorum.Jugular vein is injected usually at upper 2/3, and arteria carotis bloodletting is at lower 1/3.At
In the manufacturing process of year bovine jugular vein injection model, how to model be designed processing with it is clear intuitively show it is jugular
Position, the Attention Points that intravenous injection is presented are also a great problem for needing to face.
Summary of the invention
Object of the present invention is in order to solve the above technical problems, providing a kind of production side of bovine jugular vein injection model of growing up
Method, prepared jugular vein injection model function admirable is of a tightly knit structure, and form understands naturally, being conducive to jugular vein injection repeatedly
Operation.
A kind of production method for bovine jugular vein injection model of growing up, specific preparation step are as follows:
Step 1: taking the emulsifying wax liquid of 15-20%, the methyl-silicone oil emulsion of 15-20%, 50-55% according to percent by volume
The sodium-chloride water solution of 5mol/L, the polyacrylamide amine emulsifier of 8-13% and 1-2% o-phenyl phenol preservative；It will emulsification
Wax liquor and methyl-silicone oil emulsion are added in the sodium-chloride water solution of 5mol/L and are uniformly mixed, 60-65 DEG C of water-bath 5-10min, then
Polyacrylamide amine emulsifier and o-phenyl phenol preservative is added, stirs, is configured to release agent, it is spare；
Step 2: taking rubber powder 1-4%, powdered whiting 48-52%, sierozem power 20-25%, white cement according to mass percent
22-28% and pectin 2-5% are mixed, and reaction mixture is formed；The water of the 30-50% of its quality is added into reaction mixture,
The glycerol for adding the 3-5% of water quality used after mixing evenly, is uniformly mixed, and is configured to gypsum cream, spare；
Step 3: take molded silicon rubber, and add thereto its quality 2% condensation polymer type two-component silicone rubber crosslinking agent,
1.5% sodium carbonate and 1% glycine, stir evenly, be configured to silicon rubber, it is spare；
Step 4: peeling: ox corpse is faced upward and is put by selection Adult Bovine corpse, is cut with operating scissors in neck veutro median line small
Mouthful, it then cuts from cut osculum to head to lower lip, rearwardly cuts to thoracic cavity inlet annular and cut off；With the method for drill drift by skin
It is sloughed to head, becomes the housing type of dermatotome outwardly；The auricular cartilage of ear is cut, eye and mouth skin of lip are separated, it will
Skin completely strips；
Step 5: isolating jugular vein, arteria carotis and sympathetic trunk in ox head neck jugular vein ditch, cleared up dry
Only, be then dipped into the acetone that volume ratio is 3:1: 4h in the mixture of chloroform is gently rinsed with water, in remaining adipose tissue
Place gently wipes, and then uses picric acid saturated solution immersion treatment 0.5h；One is respectively inserted into jugular vein, arteria carotis can prop up blood vessel
The support tube of shape；Successive squeegeeing step one is prepared outside the jugular vein and arteria carotis and sympathetic trunk for being inserted with support tube
The silicon rubber that release agent and step 3 are prepared, solidifies to silicon rubber, jugular vein, arteria carotis and sympathetic trunk silicon rubber mould is made
Type；
Step 6: jugular vein ditch is cleared up dry in the ox head neck for isolating jugular vein, arteria carotis and sympathetic trunk
Only, ox head neck then is made in the gypsum cream of the successive squeegeeing step one of the ox head neck release agent prepared and step 2 preparation
Mold；
Step 7: 0.2-0.4kg ox hair is taken to be placed in glass container, 120 DEG C of high pressure steam sterilization 20min, then in 55-
20-30min is handled in 60 DEG C of drying box, after finally being rubbed with talcum powder and is handled clean；
Step 8: by ox head neck made of jugular vein, arteria carotis and sympathetic trunk silicone rubber model Embedded step six
In the jugular vein ditch of mold, the silicon rubber that then squeegeeing step three is prepared uniformly is stained with before silicon rubber solidification through at step 7
The ox hair of reason, posture when making ox head neck phantom that high head be kept to stand, is put into specimen jar, bovine jugular vein injection mold is made
Type.
As further optimization, in step 3, toner is added into molded silicon rubber, prepares au bleu, red respectively
Color and white silicon rubber；Described in step 5 when brushing silicon rubber, indigo plant is respectively adopted in jugular vein, arteria carotis and sympathetic trunk
Color, red and white silicon rubber.
As further optimization, picric acid saturated solution described in step 5 is that every 1.5 g picric acid solid is dissolved in 100
It is formulated in 20% ethyl alcohol of ml.
As further optimization, picric acid saturated solution wants matching while using or is placed in 4 DEG C to save backup.
As further optimization, support tube described in step 5 is the plastic tube that diameter is 1cm.
Optimize as further, in step 6, after brushing release agent, brushes 5 times gypsum creams.
Beneficial effect
1, release agent of the invention uses sodium-chloride water solution for dispersed phase, with emulsifying wax liquid, methyl-silicone oil emulsion, poly- third
Acrylamide emulsifier and the mixing of o-phenyl phenol preservative, wherein methyl-silicone oil emulsifier stability is good, nontoxic odorlessness, resists
Oxidation, can increase lubricity, while but also as defoaming agent；O-phenyl phenol has preferable sterilization except mould energy as preservative
Power；The present invention is added neutral salt, can be balanced the mutual exclusion in release agent between each organic principle using sodium-chloride water solution as dispersed phase
Effect, the structure alleviated the molecular action due to o-phenyl phenol and destroy polyacrylamide keep the performance of its emulsification unknown
It is aobvious, it prevents from mixing the uncoordinated function and effect for weakening release agent between each ingredient, to improve the quality level of release agent.
2, gypsum cream of the invention uses rubber powder, powdered whiting, sierozem power and white cement, and a certain amount of pectin is added,
Wherein, pectin molecule it is each in gypsum cream can to slow down hydrone with water molecules during adding water and stirring and preparing gypsum cream
Diffusion velocity between ingredient keeps effect more abundant, the more uniform exquisiteness in the gypsum breast matter of preparation ground, simultaneously because and hydrone
Combination, moisture is not volatile, can play Water-saving effect from inside；Glycerol is added, the lubricity of gypsum cream can on the one hand increased
By force, the protective film of water conservation on the other hand can be formed outside uniformly mixed gypsum cream, inside and outside collective effect increases gypsum cream
Water-saving effect prevents gypsum cream excessively dry and cracks, in addition, when the solidification of gypsum cream can be also appropriately extended in addition glycerol
Between, improve self-strength.
3, molded silicon rubber is taken in silicon rubber of the invention preparation and add 2% condensation polymer type two-component silicon rubber of its quality
Rubber cross linker, 1.5% sodium carbonate and 1% glycine, the sodium carbonate of addition are alkalescent, 2% crosslinking agent and 1.5% sodium carbonate
Collective effect can make the curing time of silicon rubber maintain 5h or so, can complete peeling and the production of model during this period of time,
Time cost will not be increased because of overlong time again simultaneously；The by-products such as methanol, ethyl alcohol can be generated in silicon rubber reaction process
Object and generation heat will affect glue connection if the by-product of generation cannot overflow in time, and the sodium carbonate of addition can promote by-product
It overflows, the heat for guaranteeing going on smoothly for cross-linking reaction, but generating in reaction process can decompose sodium carbonate production to a certain extent
Raw carbon dioxide, the glycine of addition can be complexed to form Sodium Glycinate, absorb the carbon dioxide of generation with free sodium ion,
Bubble is prevented, in addition, the crosslinking agent that the present invention uses is a kind of diacyl organic peroxide, vulnerable to external factor (temperature
Degree, humidity, oxygen etc.) influence recurring structure variation, 1% glycine, which is added, to shield to it, and each ingredient is made jointly
With can make the breaking strength of silicon rubber up to 5.6MPa within the present invention optimal curing time, elongation at break is up to 550%, hence it is evident that mentions
High silicon rubber tensile strength.
4, preparation method of the present invention is simple, low in cost, prepared jugular vein injection model, by jugular vein together with neck
Artery and sympathetic trunk are embedded in together in jugular vein ditch, and morphologic localization is true to nature naturally, beginner is facilitated to differentiate jugular phase
To position, structure snd size, it is easier to hold injection point；In addition the model is easy to save, and function admirable is visual in image, and structure is complete
It is whole, can repeatability practice, make up cattle on the hoof jugular vein and inject the deficiency that cannot repeat the operation several times, eliminate conventional teaching and rely on plane
The defect that drawing and subjective imagination are learnt, enhancing understands and memory, improves efficiency of teaching.
Specific embodiment
Explanation is further explained to the present invention below by specific embodiment.
Embodiment 1: a kind of jugular vein injection model production method of Adult Bovine, specific preparation step are as follows:
Step 1: according to percent by volume, take 15% emulsifying wax liquid, 20% methyl-silicone oil emulsion, 55% 5mol/L
Sodium-chloride water solution, 9% polyacrylamide amine emulsifier and 1% o-phenyl phenol preservative；By emulsifying wax liquid and methyl-silicone oil
Lotion is added in the sodium-chloride water solution of 5mol/L, is uniformly mixed, 60 DEG C of water-bath 5min add polyacrylamide amine emulsifier
It with o-phenyl phenol preservative, stirs, is configured to release agent, it is spare；
Step 2: taking NSJF-3B rubber powder 1%, powdered whiting 48%, sierozem power 25%, white cement according to mass percent
22% and pectin 4% mixed, formed reaction mixture；30% water of its quality is added into reaction mixture, stirs evenly
3% glycerol for adding water quality used afterwards, is uniformly mixed, and is configured to gypsum cream, spare；
Step 3: taking molded silicon rubber, and 2% condensation polymer type two-component silicone rubber crosslinking agent of its quality is added thereto
(RTV-2), 1.5% sodium carbonate and 1% glycine, add toner, stir evenly, white, red, blue and nothing is respectively prepared
The silicon rubber of color, it is spare；
Step 4: peeling: ox corpse is faced upward and is put by selection Adult Bovine corpse, is cut with operating scissors in neck veutro median line small
Mouthful, it then cuts from cut osculum to head to lower lip, rearwardly cuts to thoracic cavity inlet annular and cut off；With the method for drill drift by skin
It is sloughed to head, becomes the housing type of dermatotome outwardly；The auricular cartilage of ear is cut, eye and mouth skin of lip are separated, it will
Skin completely strips；
Step 5: isolating jugular vein, arteria carotis and sympathetic trunk in ox head neck jugular vein ditch, cleared up dry
Only, be then dipped into the acetone that volume ratio is 3:1: 4h in the mixture of chloroform is gently rinsed with water, in remaining adipose tissue
Place is gently wiped with cotton or gauze, then uses picric acid saturated solution immersion treatment 0.5h；It is respectively inserted into jugular vein, arteria carotis
One can prop up the support tube of shape of blood vessel；Successive squeegeeing step one is prepared outside jugular vein, arteria carotis and sympathetic trunk
The silicon rubber that release agent and step 3 are prepared, solidifies to silicon rubber, jugular vein, arteria carotis and sympathetic trunk silicon rubber mould is made
Type；
Wherein, the picric acid is saturated formula of liquid: every 1.5 g picric acid solid is dissolved in 100 ml, 20% ethyl alcohol；Institute
The solution of preparation wants matching while using or is placed in 4 DEG C of preservations；
Step 6: jugular vein ditch is cleared up dry in the ox head neck for isolating jugular vein, arteria carotis and sympathetic trunk
Only, then in the gypsum cream of the successive squeegeeing step one of the ox head neck release agent prepared and step 2 preparation, gypsum cream brushing 5 times
To protrude jugular vein ditch, ox head neck mold is made；
Step 7: 0.3kg ox hair is taken to be placed in glass container, 120 DEG C of high pressure steam sterilization 20min, then at 55 DEG C
20min is handled in drying box, after finally being rubbed with talcum powder and is handled clean.
Step 8: by ox head neck made of jugular vein, arteria carotis and sympathetic trunk silicone rubber model Embedded step six
In the jugular vein ditch of mold, then the brushing silica gel on ox head neck mold is uniformly stained with before silica gel solidification through at step 7
The ox hair of reason, posture when making ox head neck phantom that high head be kept to stand, is put into specimen jar, bovine jugular vein injection mold is made
Type.
Embodiment 2: a kind of jugular vein injection model production method of Adult Bovine, specific preparation step are as follows:
Step 1: according to percent by volume, take 20% emulsifying wax liquid, 20% methyl-silicone oil emulsion, 50% 5mol/L
Sodium-chloride water solution, 9% polyacrylamide amine emulsifier and 1% o-phenyl phenol preservative, by emulsifying wax liquid and methyl-silicone oil
Lotion is added in the sodium-chloride water solution of 5mol/L, is uniformly mixed, 60 DEG C of water-bath 10min, and polyacrylamide emulsification is added
Agent and o-phenyl phenol preservative, stir, and are configured to release agent, spare；
Step 2: taking NSJF-3B rubber powder 1%, powdered whiting 48%, sierozem power 21%, white cement according to mass percent
28% and pectin 2% mixed, formed reaction mixture；40% water of its quality is added into reaction mixture, stirs evenly
4% glycerol for adding water quality used afterwards, is uniformly mixed, and is configured to gypsum cream, spare；
Step 3: taking molded silicon rubber, and 2% condensation polymer type two-component silicone rubber crosslinking agent of its quality is added thereto
(RTV-2), 1.5% sodium carbonate and 1% glycine, add toner, stir evenly, white, red, blue and nothing is respectively prepared
The silicon rubber of color, it is spare；
Step 4: peeling: ox corpse is faced upward and is put by selection Adult Bovine corpse, is cut with operating scissors in neck veutro median line small
Mouthful, it then cuts from cut osculum to head to lower lip, rearwardly cuts to thoracic cavity inlet annular and cut off；With the method for drill drift by skin
It is sloughed to head, becomes the housing type of dermatotome outwardly；The auricular cartilage of ear is cut, eye and mouth skin of lip are separated, it will
Skin completely strips；
Step 5: isolating jugular vein, arteria carotis and sympathetic trunk in ox head neck jugular vein ditch, cleared up dry
Only, be then dipped into the acetone that volume ratio is 3:1: 4h in the mixture of chloroform is gently rinsed with water, in remaining adipose tissue
Place is gently wiped with cotton or gauze, then uses picric acid saturated solution immersion treatment 0.5h；It is respectively inserted into jugular vein, arteria carotis
One can prop up the support tube of shape of blood vessel；Successive squeegeeing step one is prepared outside jugular vein, arteria carotis and sympathetic trunk
The silicon rubber that release agent and step 3 are prepared, solidifies to silicon rubber, jugular vein, arteria carotis and sympathetic trunk silicon rubber mould is made
Type；
Wherein, the picric acid is saturated formula of liquid: every 1.5 g picric acid solid is dissolved in 100 ml, 20% ethyl alcohol；Institute
The solution of preparation wants matching while using or is placed in 4 DEG C of preservations；
Step 6: jugular vein ditch is cleared up dry in the ox head neck for isolating jugular vein, arteria carotis and sympathetic trunk
Only, then in the gypsum cream of the successive squeegeeing step one of the ox head neck release agent prepared and step 2 preparation, gypsum cream brushing 5 times
To protrude jugular vein ditch, ox head neck mold is made；
Step 7: 0.3kg ox hair is taken to be placed in glass container, 120 DEG C of high pressure steam sterilization 20min, then at 55 DEG C
25min is handled in drying box, after finally being rubbed with talcum powder and is handled clean.
Step 8: by ox head neck made of jugular vein, arteria carotis and sympathetic trunk silicone rubber model Embedded step six
In the jugular vein ditch of mold, then the brushing silica gel on ox head neck mold is uniformly stained with before silica gel solidification through at step 7
The ox hair of reason, posture when making ox head neck phantom that high head be kept to stand, is put into specimen jar, bovine jugular vein injection mold is made
Type.
Embodiment 3: a kind of jugular vein injection model production method of Adult Bovine, specific preparation step are as follows:
Step 1: according to percent by volume, take 15% emulsifying wax liquid, 15% methyl-silicone oil emulsion, 55% 5mol/L
Sodium-chloride water solution, 13% polyacrylamide amine emulsifier and 2% o-phenyl phenol preservative；By emulsifying wax liquid and methyl-silicone oil
Lotion is added in the sodium-chloride water solution of 5mol/L, is uniformly mixed, 63 DEG C of water-bath 8min add polyacrylamide amine emulsifier
It with o-phenyl phenol preservative, stirs, is configured to release agent, it is spare；
Step 2: taking NSJF-3B rubber powder 3%, powdered whiting 50%, sierozem power 20%, white cement according to mass percent
22% and pectin 5% mixed, formed reaction mixture；40% water of its quality is added into reaction mixture, stirs evenly
4% glycerol for adding water quality used afterwards, is uniformly mixed, and is configured to gypsum cream, spare；
Step 3: taking molded silicon rubber, and 2% condensation polymer type two-component silicone rubber crosslinking agent of its quality is added thereto
(RTV-2), 1.5% sodium carbonate and 1% glycine, add toner, stir evenly, white, red, blue and nothing is respectively prepared
The silicon rubber of color, it is spare；
Step 4: peeling: ox corpse is faced upward and is put by selection Adult Bovine corpse, is cut with operating scissors in neck veutro median line small
Mouthful, it then cuts from cut osculum to head to lower lip, rearwardly cuts to thoracic cavity inlet annular and cut off；With the method for drill drift by skin
It is sloughed to head, becomes the housing type of dermatotome outwardly；The auricular cartilage of ear is cut, eye and mouth skin of lip are separated, it will
Skin completely strips；
Step 5: isolating jugular vein, arteria carotis and sympathetic trunk in ox head neck jugular vein ditch, cleared up dry
Only, be then dipped into the acetone that volume ratio is 3:1: 4h in the mixture of chloroform is gently rinsed with water, in remaining adipose tissue
Place is gently wiped with cotton or gauze, then uses picric acid saturated solution immersion treatment 0.5h；It is respectively inserted into jugular vein, arteria carotis
One can prop up the support tube of shape of blood vessel；Successive squeegeeing step one is prepared outside jugular vein, arteria carotis and sympathetic trunk
The silicon rubber that release agent and step 3 are prepared, solidifies to silicon rubber, jugular vein, arteria carotis and sympathetic trunk silicon rubber mould is made
Type；
Wherein, the picric acid is saturated formula of liquid: every 1.5 g picric acid solid is dissolved in 100 ml, 20% ethyl alcohol；Institute
The solution of preparation wants matching while using or is placed in 4 DEG C of preservations；
Step 6: jugular vein ditch is cleared up dry in the ox head neck for isolating jugular vein, arteria carotis and sympathetic trunk
Only, then in the gypsum cream of the successive squeegeeing step one of the ox head neck release agent prepared and step 2 preparation, gypsum cream brushing 5 times
To protrude jugular vein ditch, ox head neck mold is made；
Step 7: 0.3kg ox hair is taken to be placed in glass container, 120 DEG C of high pressure steam sterilization 20min, then at 55 DEG C
30min is handled in drying box, after finally being rubbed with talcum powder and is handled clean.
Step 8: by ox head neck made of jugular vein, arteria carotis and sympathetic trunk silicone rubber model Embedded step six
In the jugular vein ditch of mold, then the brushing silica gel on ox head neck mold is uniformly stained with before silica gel solidification through at step 7
The ox hair of reason, posture when making ox head neck phantom that high head be kept to stand, is put into specimen jar, bovine jugular vein injection mold is made
Type.
Embodiment 4: a kind of jugular vein injection model production method of Adult Bovine, specific preparation step are as follows:
Step 1: according to percent by volume, take 20% emulsifying wax liquid, 16% methyl-silicone oil emulsion, 54% 5mol/L
Sodium-chloride water solution, 8% polyacrylamide amine emulsifier and 2% o-phenyl phenol preservative；By emulsifying wax liquid and methyl-silicone oil
Lotion is added in the sodium-chloride water solution of 5mol/L, is uniformly mixed, 65 DEG C of water-bath 10min, and polyacrylamide emulsification is added
Agent and o-phenyl phenol preservative, stir, and are configured to release agent, spare；
Step 2: taking NSJF-3B rubber powder 4%, powdered whiting 52%, sierozem power 20%, white cement according to mass percent
22% and pectin 2% mixed, formed reaction mixture；50% water of its quality is added into reaction mixture, stirs evenly
5% glycerol for adding water quality used afterwards, is uniformly mixed, and is configured to gypsum cream, spare；
Step 3: taking molded silicon rubber, and 2% condensation polymer type two-component silicone rubber crosslinking agent of its quality is added thereto
(RTV-2), 1.5% sodium carbonate and 1% glycine, add toner, stir evenly, white, red, blue and nothing is respectively prepared
The silicon rubber of color, it is spare；
Step 4: peeling: ox corpse is faced upward and is put by selection Adult Bovine corpse, is cut with operating scissors in neck veutro median line small
Mouthful, it then cuts from cut osculum to head to lower lip, rearwardly cuts to thoracic cavity inlet annular and cut off；With the method for drill drift by skin
It is sloughed to head, becomes the housing type of dermatotome outwardly；The auricular cartilage of ear is cut, eye and mouth skin of lip are separated, it will
Skin completely strips；
Step 5: isolating jugular vein, arteria carotis and sympathetic trunk in ox head neck jugular vein ditch, cleared up dry
Only, be then dipped into the acetone that volume ratio is 3:1: 4h in the mixture of chloroform is gently rinsed with water, in remaining adipose tissue
Place is gently wiped with cotton or gauze, then uses picric acid saturated solution immersion treatment 0.5h；It is respectively inserted into jugular vein, arteria carotis
One can prop up the support tube of shape of blood vessel；Successive squeegeeing step one is prepared outside jugular vein, arteria carotis and sympathetic trunk
The silicon rubber that release agent and step 3 are prepared, solidifies to silicon rubber, jugular vein, arteria carotis and sympathetic trunk silicon rubber mould is made
Type；
Wherein, the picric acid is saturated formula of liquid: every 1.5 g picric acid solid is dissolved in 100 ml, 20% ethyl alcohol；Institute
The solution of preparation wants matching while using or is placed in 4 DEG C of preservations；
Step 6: jugular vein ditch is cleared up dry in the ox head neck for isolating jugular vein, arteria carotis and sympathetic trunk
Only, then in the gypsum cream of the successive squeegeeing step one of the ox head neck release agent prepared and step 2 preparation, gypsum cream brushing 5 times
To protrude jugular vein ditch, ox head neck mold is made；
Step 7: 0.3kg ox hair is taken to be placed in glass container, 120 DEG C of high pressure steam sterilization 20min, then at 55 DEG C
28min is handled in drying box, after finally being rubbed with talcum powder and is handled clean.
Step 8: by ox head neck made of jugular vein, arteria carotis and sympathetic trunk silicone rubber model Embedded step six
In the jugular vein ditch of mold, then the brushing silica gel on ox head neck mold is uniformly stained with before silica gel solidification through at step 7
The ox hair of reason, posture when making ox head neck phantom that high head be kept to stand, is put into specimen jar, bovine jugular vein injection mold is made
Type.
Related experiment: the optimum proportioning of each adding ingredient in silicon rubber is determined using orthogonal test
In the manufacturing process of mold, silicon rubber must have its unique performance, in order to meet the needs of this experimental study,
The performance of silicon rubber is evaluated by curing time and tensile strength.
Curing time measurement: being processed into thickness 6mm for silicon rubber first, the rectangle sample of width 15mm, length 35mm,
It is required that specimen surface is smooth, face is parallel corresponding thereto for test surfaces, at room temperature, sample is placed in smooth glass plate work
Make on platform, test surfaces are parallel to the ground.With vertical force by the pressing pressing in sample surface of Shore A durometer, until the pressure of spring
Power balances each other with counter-force, reads the dial corresponding readings of pointer meaning, measures shore hardness, wherein each point is only surveyed once,
Each sample surveys three points, and distance is no less than 10mm between three points, and final result takes intermediate value；When shore hardness is measured as 35HA
Time rule used is the curing time of silicon rubber.
Stretching strength determination: in normal state, after waiting silicon rubber to be fully cured, punch press process is tried at the stretching of standard
Sample is placed in the center of clamper by sample, i.e. dumbbell shaped, records sample initial gage, and be switched on tensile sample, and record sample is pulled apart
When gauge length when pulling apart of load and sample.The tensile strength of silicon rubber is generally characterized with breaking strength and elongation at break.
Wherein, the calculation formula of breaking strength is a=F/bd, in formula: a-breaking strength, MPa；The suffered effect of F-sample
Power, N；B-working portion width, m；D-working portion thickness, m；As a result it is indicated with the intermediate value of 3 samples.
The calculation formula of elongation at break is l=[(L-L0)/L0] * 100%, in formula: l-elongation at break, %；L-sample
Gauge length when pulling apart, mm；L0- sample initial gage, mm；As a result it is indicated with the intermediate value of 3 samples.
Orthogonal test scheme is designed, accounts for the quality of molded silicon rubber respectively with three kinds of RTV-2, sodium carbonate and glycine ingredients
Percentage determines four levels of each factor by single factor experiment result, such as three factors for influencing silicon rubber quality
Shown in table 1.
1 orthogonal test factor level table of table
According to above-mentioned influence silicon rubber quality because of prime number and number of levels, using L16(45) design orthogonal test, with solidification
Time, breaking strength and elongation at break are test testing index, as a result as shown in table 2 below.
2 orthogonal test measurement result table of table
The analysis of 3 orthogonal test curing time measurement result of table
Using curing time as testing index, the factor sequence for influencing curing time is A > B > C, and the content of glycine is to silicon rubber
The influence of adhesive curing time is little.
The analysis of 4 orthogonal test breaking strength measurement result of table
Using breaking strength as testing index, the factor sequence for influencing breaking strength is C > A > B.
The analysis of 5 orthogonal test elongation at break measurement result of table
Using elongation at break as testing index, the factor sequence for influencing elongation at break is C > A > B.Comprehensive curing time,
Influence of three factors of breaking strength and elongation at break to Silicone Rubber is horizontal, and the optimum proportioning of three factors is A4B3C2,
Added in molded silicon rubber the condensation polymer type two-component silicone rubber crosslinking agent of the 2% of its quality, 1.5% sodium carbonate and 1% it is sweet
Propylhomoserin.
Claims (6)
1. a kind of production method for bovine jugular vein injection model of growing up, it is characterised in that: the following steps are included:
Step 1: according to percent by volume, the emulsifying wax liquid of 15-20%, the methyl-silicone oil emulsion of 15-20%, 50-55% are taken
The o-phenyl phenol preservative of the sodium-chloride water solution of 5mol/L, the polyacrylamide amine emulsifier of 8-13% and 1-2%；By emulsifying wax
Liquid and methyl-silicone oil emulsion are added in the sodium-chloride water solution of 5mol/L and are uniformly mixed, 60-65 DEG C of water-bath 5-10min, then plus
Enter polyacrylamide amine emulsifier and o-phenyl phenol preservative, stir, is configured to release agent, it is spare；
Step 2: taking rubber powder 1-4%, powdered whiting 48-52%, sierozem power 20-25%, white cement 22- according to mass percent
28% and pectin 2-5% is mixed, and reaction mixture is formed；The water of the 30-50% of its quality is added into reaction mixture, stirs
The glycerol that the 3-5% of water quality used is added after mixing uniformly, is uniformly mixed, and is configured to gypsum cream, spare；
Step 3: taking molded silicon rubber, and 2% condensation polymer type two-component silicone rubber crosslinking agent of its quality, 1.5% are added thereto
Sodium carbonate and 1% glycine, stir evenly, be configured to silicon rubber, it is spare；
Step 4: peeling: ox corpse is faced upward and is put by selection Adult Bovine corpse, is cut an osculum in neck veutro median line with operating scissors, is connect
Cut from cut osculum to head to lower lip, rearwardly cut to thoracic cavity inlet annular and cut off；With the method for drill drift by Pi Xiangtou
Portion is sloughed, and becomes the housing type of dermatotome outwardly；The auricular cartilage of ear is cut, eye and mouth skin of lip are separated, skin is complete
Whole removing；
Step 5: isolating jugular vein, arteria carotis and sympathetic trunk in ox head neck jugular vein ditch, cleaned out,
Then be dipped into the acetone that volume ratio is 3:1: 4h in the mixture of chloroform is gently rinsed with water, at remaining adipose tissue
It gently wipes, then uses picric acid saturated solution immersion treatment 0.5h；One is respectively inserted into jugular vein, arteria carotis can prop up blood vessel shape
The support tube of shape；What successive squeegeeing step one was prepared outside the jugular vein and arteria carotis and sympathetic trunk for being inserted with support tube is de-
The silicon rubber that mould agent and step 3 are prepared, solidifies to silicon rubber, jugular vein, arteria carotis and sympathetic trunk silicon rubber mould is made
Type；
Step 6: jugular vein ditch is cleaned out, so in the ox head neck for isolating jugular vein, arteria carotis and sympathetic trunk
Afterwards in the gypsum cream of the successive squeegeeing step one of the ox head neck release agent prepared and step 2 preparation, ox head neck mold is made；
Step 7: 0.2-0.4kg ox hair is taken to be placed in glass container, 120 DEG C of high pressure steam sterilization 20min, then at 55-60 DEG C
Drying box in handle 20-30min, after finally being rubbed with talcum powder and handle clean；
Step 8: by ox head neck mold made of jugular vein, arteria carotis and sympathetic trunk silicone rubber model Embedded step six
Jugular vein ditch in, the then brushing silica gel on ox head neck mold is uniformly stained with and is handled through step 7 before the silica gel solidification
Ox hair, posture when making ox head neck phantom that high head be kept to stand, is put into specimen jar, bovine jugular vein injection model is made.
2. a kind of production method of bovine jugular vein injection model of growing up as described in claim 1, it is characterised in that: to step 3
Molded silicon rubber in add toner, prepare au bleu, red and white silicon rubber respectively；The brushing silicon rubber described in step 5
When glue, blue, red and white silicon rubber is respectively adopted in jugular vein, arteria carotis and sympathetic trunk.
3. a kind of production method of bovine jugular vein injection model of growing up as described in claim 1, it is characterised in that: step 5 institute
Stating picric acid saturated solution is that every 1.5 g picric acid solid is dissolved in 20% ethyl alcohol of 100ml to be formulated.
4. a kind of production method of bovine jugular vein injection model of growing up as claimed in claim 1 or 3, it is characterised in that: bitter taste
Sour saturated solution wants matching while using or is placed in 4 DEG C to save backup.
5. a kind of production method of bovine jugular vein injection model of growing up as described in claim 1, it is characterised in that: in step 5
The support tube is the plastic tube that diameter is 1cm.
6. a kind of production method of bovine jugular vein injection model of growing up as described in claim 1, it is characterised in that: step 6
In, after brushing release agent, brush 5 times gypsum creams.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2570904Y (en) * | 2002-09-19 | 2003-09-03 | 刘霄飞 | Torso training model for central intravenous injection |
| CN201017514Y (en) * | 2006-12-14 | 2008-02-06 | 天津市天堰医教科技开发有限公司 | Torso intravenous injection training model |
| CN201145981Y (en) * | 2007-12-13 | 2008-11-05 | 天津市天堰医教科技开发有限公司 | Center intravenous injection model |
| CN105280071A (en) * | 2015-12-03 | 2016-01-27 | 河南科技大学 | Method for manufacturing pig ear intravenous injection model based on mathematical modeling |
| CN105355127A (en) * | 2015-12-03 | 2016-02-24 | 河南科技大学 | Mathematical modeling-based adult rat tail vein injection model fabricating method |
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Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2570904Y (en) * | 2002-09-19 | 2003-09-03 | 刘霄飞 | Torso training model for central intravenous injection |
| CN201017514Y (en) * | 2006-12-14 | 2008-02-06 | 天津市天堰医教科技开发有限公司 | Torso intravenous injection training model |
| CN201145981Y (en) * | 2007-12-13 | 2008-11-05 | 天津市天堰医教科技开发有限公司 | Center intravenous injection model |
| CN105280071A (en) * | 2015-12-03 | 2016-01-27 | 河南科技大学 | Method for manufacturing pig ear intravenous injection model based on mathematical modeling |
| CN105355127A (en) * | 2015-12-03 | 2016-02-24 | 河南科技大学 | Mathematical modeling-based adult rat tail vein injection model fabricating method |
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