CN104655460B - A kind of transparent dewaxing liquid of pathological tissue and preparation method thereof - Google Patents
A kind of transparent dewaxing liquid of pathological tissue and preparation method thereof Download PDFInfo
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Abstract
The invention discloses transparent dewaxing liquid of a kind of pathological tissue and preparation method thereof, including the parts by weight of height side chain saturated alkane 92.5 98, the parts by weight of stabilizer 0.5 5 and the parts by weight of synergist 0.1 2.5；The height side chain saturated alkane is naphthenic oil, quadricyclane, along one or more of mixtures in the hexahydrotoluene of 1 isopropyl 4, the anti-hexahydrotoluene of 1 isopropyl 4；Described stabilizer is one or more of mixtures in epoxidized soybean oil, trimethylolpropane, butyl epoxy stearate；Described synergist is one or more of mixtures in polyether-modified heptamethyltrisiloxane, butyl ether dimethyl silicone polymer, the hydroxypropyl methyl of dimethyl 3 (siloxanes and polysiloxanes).Solve that the transparent dewaxing liquid of pathological tissue in the prior art is poisonous and irritant smell, immersion tissue can make tissue become fragile and shrink the technical problem of unfavorable section for a long time.
Description
Technical field
The invention belongs to pathological tissue microtomy field, and in particular to a kind of transparent dewaxing liquid of pathological tissue and its system
Preparation Method and application.
Background technology
At present, the manufacturing process of most histopathologic slides still uses traditional Technique of Paraffin Section, its mistake
Journey include fixed, dehydration, for the first time the step such as transparent, waxdip, section, dewaxing, dyeing, second transparent, mounting processing
The making of pathological section can be completed.Wherein, the first time transparent instrumentality referred to by transparent liquid, and reach paraffin immersion group
The purpose of block is knitted, during this, the hydrophilic component in tissue block is replaced by transparent liquid so that tissue block becomes bright；And take off
Wax refers to before tissue section strain that the paraffin in section must be dissolved by dewaxing liquid, so that dyestuff is easy to immerse tissue and cell
Operation；It is transparent for the second time to refer to that histotomy be handled after dyeing, dehydration through transparent liquid, make section transparent, in favor of
Light is passed through, ability mounting, and otherwise institutional framework is smudgy under the microscope, and this is transparent second.Transparent, dewaxing effect
Quality directly influence the quality of paraffin wax flaking.In the manufacturing process of histopathologic slide, the transparent and section dye of tissue
Dewaxing before and after color, transparent three links are, it is necessary to a large amount of transparent deparaffinization reagents.
The most frequently used transparency of organization dewaxing liquid is mainly dimethylbenzene at present, and dimethylbenzene is in the manual film-making of pathology department, automatic de-
Largely used in water dispenser and automatic staining machine.It is due to diformazan although dimethylbenzene has good dewaxing and transparent effect
Benzene belongs to the neurotoxin with intense irritation smell, and its toxicity is mainly Central nervous and neuro vegetative anesthesia and viscous
The stimulation of film, the selective special injury of personnel's physiological system to Long Term Contact produces target organ effect, can gone out
Lose face and the obvious irritation of the upper respiratory tract, eye conjunctiva and pharyngeal hyperemia, dizziness, Nausea and vomiting, uncomfortable in chest, weakness of limbs, consciousness
The symptom such as fuzzy, in addition, can also cause the symptoms such as immunologic hypofunction, anaemia, dermatitis, eczema and dry skin, to pathology work
The healthy generation for making personnel seriously endangers and causes environmental pollution, and Health cost caused by dimethylbenzene has been included in China's duty
Industry disease poisoning scope, and in pathological tissue processing procedure, use dimethylbenzene easily to cause pathological tissue to shrink, harden change
Crisp, therefore, healthy for related experiment personnel, dimethylbenzene is simultaneously not suitable for human body, animal tissue's pathological section system
Transparent, dewaxing during standby are used.
Although there is substitute of the dewaxing liquid using turpentine oil as main component as dimethylbenzene dewaxing liquid in the prior art,
It has certain transparent dewaxing effect, but the unpleasant peculiar smell of turpentine oil itself still makes it difficult for people to receive, and adds turpentine oil natural
The exhaustion of resource, this only has the substitute applied on a small quantity and has essentially dropped out histopathologic slide's making industry；Stearic acid was also once
Through being used for transparent dewaxing, but because its easy flake, the reasons such as transparent not good enough, long-term storage wax stone tissue contracts of cutting into slices are not obtained
To extensive use.
The content of the invention
Therefore, the technical problems to be solved by the invention are that the transparent dewaxing liquid of pathological tissue is poisonous in the prior art and has
Penetrating odor, immersion tissue can make tissue become fragile and shrink unfavorable section for a long time, and then provide a kind of nontoxic, nonirritant
Smell and for a long time immersion tissue will not make that tissue becomes fragile and the transparent dewaxing liquid of pathological tissue that shrinks and preparation method thereof is with answering
With.
In order to solve the above technical problems, the transparent dewaxing liquid of pathological tissue of the present invention, includes the component of following parts by weight：It is high
Spend side chain saturated alkane 92.5-98 parts by weight, stabilizer 0.5-5 parts by weight and synergist 0.1-2.5 parts by weight.
It is preferred that, the transparent dewaxing liquid of pathological tissue includes the component of following parts by weight：Height side chain saturated alkane is
95-97 parts by weight, stabilizer 2-3 parts by weight and synergist 1-2 parts by weight.
It is preferred that, the height side chain saturated alkane is naphthenic oil, quadricyclane, cis- 1- isopropyl-4-methyls hexamethylene
One or more of mixtures in alkane, trans- 1- isopropyl-4-methyls hexamethylene；
It is preferred that, described stabilizer be one kind in epoxidized soybean oil, trimethylolpropane, butyl epoxy stearate or
Several mixtures；
It is preferred that, described synergist is polyether-modified heptamethyltrisiloxane（CAS：27306-78-1）, butyl ether gather
Dimethyl siloxane (CAS:67762-87-2), dimethyl -3- hydroxypropyl methyls (siloxanes and polysiloxanes) (CAS:
One or more of mixtures in 117272-76-1).
In the above-mentioned transparent dewaxing liquid of pathological tissue, the transparent dewaxing liquid composition of pathological tissue is：
The parts by weight of naphthenic oil 98, the parts by weight of epoxidized soybean oil 0.5, the parts by weight of polyether-modified heptamethyltrisiloxane 1.5；Or
The parts by weight of quadricyclane 94.9, the parts by weight of trimethylolpropane 5, the parts by weight of butyl ether dimethyl silicone polymer 0.1；
Or
The cis- parts by weight of 1- isopropyl-4-methyls hexamethylene 92.5, the parts by weight of butyl epoxy stearate 5, dimethyl -3- hydroxyls
Hydroxypropyl methyl (siloxanes and polysiloxanes) 2.5 parts by weight；Or
The trans- parts by weight of 1- isopropyl-4-methyls hexamethylene 97.5, the parts by weight of butyl epoxy stearate 0.5, dimethyl -3-
Hydroxypropyl methyl (siloxanes and polysiloxanes) 2 parts by weight；Or
The parts by weight of naphthenic oil 96, the parts by weight of butyl epoxy stearate 3.5, the parts by weight of butyl ether dimethyl silicone polymer 0.5.
A kind of method for preparing the transparent dewaxing liquid of above-mentioned pathological tissue, including take the height branch according to selected parts by weight
Chain saturated alkane, stabilizer and synergist, the step of room temperature is reconciled to 60 DEG C.
The above-mentioned transparent dewaxing liquid of pathological tissue is making the application in pathological tissue microtomy field.
A kind of preparation method of pathological tissue paraffin section, including with the above-mentioned transparent dewaxing liquid of pathological tissue to pathologic group
Knit progress transparent for the first time, dewaxing and second of transparent step.
In the preparation method of above-mentioned pathological tissue paraffin section, the hydrodewaxing step is specially：By the disease handled well
Reason tissue paraffin section de is put into the described transparent dewaxing liquid of pathological tissue, 10-15 points of the constant temperature immersion at a temperature of 20-28 DEG C
Clock, repeats immersion 2 times；
In the preparation method of above-mentioned pathological tissue paraffin section, transparent specific steps of described first time：By wine
Pathological tissue after essence dehydration is put into the described transparent dewaxing liquid of pathological tissue, and constant temperature soaks 40-50 at a temperature of 32-37 DEG C
Minute, preferably 45 minutes, repeat immersion 1 time；
Second described of transparent specific steps：Pathological tissue after dehydration of alcohol is put into described pathological tissue saturating
In bright dewaxing liquid, constant temperature soaks 2-5 minutes at a temperature of 20-28 DEG C, preferably 3 minutes, repeats immersion 2 times.
The above-mentioned technical proposal of the present invention has advantages below compared with prior art：
（1）The transparent dewaxing liquid of pathological tissue of the present invention, including height side chain saturated alkane 92.5-98 parts by weight,
Stabilizer 0.5-5 parts by weight and synergist 0.1-2.5 parts by weight；The transparent dewaxing liquid of pathological tissue of the present invention is nontoxic, nothing
Penetrating odor, and the transparent dewaxing liquid of pathological tissue of the present invention can be used for transparent, can be used to dewax, with dimethylbenzene
Transparent, dewaxing effect is the ideal substitute of clinically dimethylbenzene quite；
（2）The transparent dewaxing liquid of pathological tissue of the present invention, by screening the obtained height side chain saturated alkane
For naphthenic oil, quadricyclane, cis- 1- isopropyl-4-methyls hexamethylene, trans- 1- isopropyl-4-methyls hexamethylene；Described is steady
Agent is determined for epoxidized soybean oil, trimethylolpropane, butyl epoxy stearate；Described synergist is the polyether-modified silicon of seven methyl three
Oxygen alkane, butyl ether dimethyl silicone polymer, dimethyl -3- hydroxypropyl methyls；So that the transparent dewaxing liquid of pathological tissue of the present invention
It is nontoxic, have no irritating odor, through Mouse Acute Toxicity given low 32000mg/kg body weight, better than the product of the World Health Organization
Micro- malicious standard（5000mg/kg）, and by experimental example, the transparent dewaxing liquid of pathological tissue of the invention and dimethylbenzene it is transparent
Dewaxing effect is suitable, and the transparent dewaxing liquid of pathological tissue of the present invention can soak pathological tissue for a long time, will not become tissue
Crisp and contraction, can strengthen waxdip effect, be conducive to section, can normally use in a humid environment；
（3）The transparent dewaxing liquid of pathological tissue of the present invention, by adjusting height side chain saturated alkane, stabilizer and increasing
The addition of agent is imitated, the transparent dewaxing effect for obtaining pathological tissue is more significant, and tissue cellularity is clear, background contrasts are high,
Can long-term storage, on tissue antigen without influence, be applicable to clinical make pathological tissue section；
（4）The transparent dewaxing liquid of pathological tissue of the present invention carries out transparent for the first time, dewaxing and second to pathological tissue
Secondary transparent operation, and screened the suitable soaking temperature used and soak time, obtains that tissue cellularity is clear, background
The high notable technique effect of contrast.
Experimental example 1
Below by way of experimental example be better described it is of the invention compared with transparent dewaxing liquid dimethylbenzene of the prior art it is transparent,
Dewaxing effect is essentially identical, and the transparent dewaxing liquid of the present invention has notable technique effect that is nontoxic, having no irritating odor.
1. experiment material
Experiment material：Fibroid smooth muscle tissue and enteric epithelium biochemistry tissue.
Experiment reagent：The transparent dewaxing liquid of test group is the transparent dewaxing liquid for preparing in the present embodiment 1；Control group it is transparent
Dewaxing liquid is dimethylbenzene.
2. experimental method and result：
2.1 experimental method
Above-mentioned pathological tissue is conventionally fabricated to paraffin section by test group and control group, differs only in examination
First time for testing in the transparent dewaxing liquid that is used of group and paraffin section manufacturing process is transparent, dewaxing, second of transparent step for
First time in the transparent dewaxing liquid of pathological tissue and the manufacturing process of the paraffin section of embodiment 1 prepared by the embodiment of the present invention 1
Transparent, dewaxing, second of transparent step, the first time transparent step is：Pathological tissue after dehydration of alcohol is put into
In the transparent dewaxing liquid of pathological tissue, constant temperature soaks 45 minutes at a temperature of 32 DEG C, repeats immersion 1 time；The hydrodewaxing step
For：The pathological tissue paraffin section handled well is put into the described transparent dewaxing liquid of pathological tissue, the constant temperature at a temperature of 28 DEG C
Immersion 10 minutes, repeats immersion 2 times；Described second transparent step be：Pathological tissue after dehydration of alcohol is put into described
The transparent dewaxing liquid of pathological tissue in, at a temperature of 20 DEG C constant temperature soak 3 minutes, repeat immersion 2 times.
2.2 result
2.2.1 to the observation of fibroid smooth muscle tissue
The fibroid smooth muscle that the fibroid smooth muscle tissue paraffin section and control group that test group is made make
Tissue paraffin section de it was found that, test group is transparent compared with control group, dewaxing effect is essentially identical, and such as test group is of the invention
The transparent dewaxing liquid of the pathological tissue of embodiment 1 and the pathology figure of control group dimethylbenzene processing, are shown in Fig. 1 and Fig. 2.
2.2.2 to the observation of enteric epithelium biochemistry tissue
The enteric epithelium biochemistry tissue that the fibroid smooth muscle tissue paraffin section and control group that test group is made make
Paraffin section it was found that, test group is transparent compared with control group, dewaxing effect is essentially identical, such as test group with the present invention implement
The transparent dewaxing liquid of the pathological tissue of example 1 and the pathology figure of control group dimethylbenzene processing, are shown in Fig. 3 and Fig. 4.
3. conclusion
The transparent of paraffin section of the pathological tissue transparent dewaxing liquid processing of the present invention, dewaxing effect and dimethylbenzene it is saturating
Bright, dewaxing effect quite, the transparent dewaxing liquid of pathological tissue of the invention can substituted dimethyl benzene completely, as the nontoxic, nothing of one kind
The transparent dewaxing liquid of environment-friendly type pathological tissue of penetrating odor.
Brief description of the drawings
In order that present disclosure is more likely to be clearly understood, specific embodiment and combination below according to the present invention
Accompanying drawing, the present invention is further detailed explanation, wherein
Fig. 1 is the pathology of the fibroid smooth muscle tissue of the transparent dewaxing liquid processing of pathological tissue of the embodiment of the present invention 1
Figure；
Fig. 2 is the pathology figure of the fibroid smooth muscle tissue of the control group dimethylbenzene of experimental example 1 processing of the present invention；
Fig. 3 is the pathology figure of the enteric epithelium biochemistry tissue of the transparent dewaxing liquid processing of pathological tissue of the embodiment of the present invention 1；
Fig. 4 is the pathology figure of the enteric epithelium biochemistry tissue of the control group dimethylbenzene of experimental example 1 processing of the present invention.
Embodiment
Embodiment 1
The transparent dewaxing liquid of pathological tissue of the present embodiment, including：
Naphthenic oil 92.5kg；
Trimethylolpropane 5kg；
Dimethyl -3- hydroxypropyl methyls (siloxanes and polysiloxanes) 0.1kg；
Above-mentioned height side chain saturated alkane, stabilizer and synergist are formed into the pathologic group in room temperature to blending at 60 DEG C
Knit transparent dewaxing liquid.
The transparent dewaxing liquid of pathological tissue of above-mentioned preparation is taken to carry out transparent for the first time, dewaxing to pathological tissue and saturating for the second time
Bright step：The first time transparent step is：Pathological tissue after dehydration of alcohol is put into the pathological tissue transparent de-
In wax liquor, constant temperature soaks 45 minutes at a temperature of 32 DEG C, repeats immersion 1 time；The hydrodewaxing step is specially：By the disease handled well
Reason tissue paraffin section de is put into the described transparent dewaxing liquid of pathological tissue, and constant temperature soaks 10 minutes at a temperature of 28 DEG C, is repeated
Immersion 2 times；Described second transparent step be：Pathological tissue after dehydration of alcohol is put into described pathological tissue transparent de-
In wax liquor, constant temperature soaks 3 minutes at a temperature of 20 DEG C, repeats immersion 2 times.
Embodiment 2
The transparent dewaxing liquid of pathological tissue of the present embodiment, including：
Quadricyclane 98kg；
Epoxidized soybean oil 0.5kg；
Polyether-modified heptamethyltrisiloxane 2.5kg.
Above-mentioned height side chain saturated alkane, stabilizer and synergist are formed into the pathologic group in room temperature to blending at 60 DEG C
Knit transparent dewaxing liquid.
The transparent dewaxing liquid of pathological tissue of above-mentioned preparation is taken to carry out transparent for the first time, dewaxing to pathological tissue and saturating for the second time
Bright step：The first time transparent step is:Pathological tissue after dehydration of alcohol is put into described pathological tissue transparent de-
In wax liquor, constant temperature soaks 45 minutes at a temperature of 37 DEG C, repeats immersion 1 time；The hydrodewaxing step is specially：By the disease handled well
Reason tissue paraffin section de is put into the described transparent dewaxing liquid of pathological tissue, and constant temperature soaks 15 minutes at a temperature of 20 DEG C, is repeated
Immersion 2 times；Described second transparent step be：Pathological tissue after dehydration of alcohol is put into described pathological tissue transparent de-
In wax liquor, constant temperature soaks 3 minutes at a temperature of 28 DEG C, repeats immersion 2 times.
Embodiment 3
The transparent dewaxing liquid of pathological tissue of the present embodiment, including：
Cis- 1- isopropyl-4-methyls hexamethylene 95kg；
Butyl epoxy stearate 3kg；
Butyl ether dimethyl silicone polymer 1kg.
Above-mentioned height side chain saturated alkane, stabilizer and synergist are formed into the pathologic group in room temperature to blending at 60 DEG C
Knit transparent dewaxing liquid.
The transparent dewaxing liquid of pathological tissue of above-mentioned preparation is taken to carry out transparent for the first time, dewaxing to pathological tissue and saturating for the second time
Bright step：Transparent step of described first time is：Pathological tissue after dehydration of alcohol is put into described pathological tissue saturating
In bright dewaxing liquid, constant temperature soaks 50 minutes at a temperature of 34 DEG C, repeats immersion 1 time;The hydrodewaxing step is specially：It will handle well
Pathological tissue paraffin section be put into the described transparent dewaxing liquid of pathological tissue, constant temperature soaks 13 minutes at a temperature of 24 DEG C,
Repeat immersion 2 times；The second transparent step is：Pathological tissue after dehydration of alcohol is put into described pathological tissue transparent
In dewaxing liquid, constant temperature soaks 5 minutes at a temperature of 24 DEG C, repeats immersion 2 times.
Embodiment 4
The transparent dewaxing liquid of pathological tissue of the present embodiment, including：
Trans- 1- isopropyl-4-methyls hexamethylene 97kg；
Trimethylolpropane 2kg；
Dimethyl -3- hydroxypropyl methyls (siloxanes and polysiloxanes) 2kg.
Above-mentioned height side chain saturated alkane, stabilizer and synergist are formed into the pathologic group in room temperature to blending at 60 DEG C
Knit transparent dewaxing liquid.
The transparent dewaxing liquid of pathological tissue of above-mentioned preparation is taken to carry out transparent for the first time, dewaxing to pathological tissue and saturating for the second time
Bright step：The first time transparent step is：Pathological tissue after dehydration of alcohol is put into described pathological tissue transparent
In dewaxing liquid, constant temperature soaks 45 minutes at a temperature of 30 DEG C, repeats immersion 2 times；The hydrodewaxing step is specially：By what is handled well
Pathological tissue paraffin section is put into the described transparent dewaxing liquid of pathological tissue, and constant temperature soaks 11 minutes at a temperature of 27 DEG C, weight
Soak 2 times again；The second transparent step is:Pathological tissue after dehydration of alcohol is put into described pathological tissue transparent de-
In wax liquor, constant temperature soaks 2 minutes at a temperature of 28 DEG C, repeats immersion 1 time.
Embodiment 5
The transparent dewaxing liquid of pathological tissue of the present embodiment, including
Naphthenic oil 98kg；
Epoxidized soybean oil 0.5kg；
Polyether-modified heptamethyltrisiloxane 1.5kg.
Above-mentioned height side chain saturated alkane, stabilizer and synergist are formed into the pathologic group in room temperature to blending at 60 DEG C
Knit transparent dewaxing liquid.
The transparent dewaxing liquid of pathological tissue of above-mentioned preparation is taken to carry out transparent for the first time, dewaxing to pathological tissue and secondary transparent
The step of：The first time transparent step is：Pathological tissue after dehydration of alcohol is put into described pathological tissue transparent de-
In wax liquor, constant temperature soaks 40 minutes at a temperature of 37 DEG C, repeats immersion 1 time;The hydrodewaxing step is specially：By the disease handled well
Reason tissue paraffin section de is put into the described transparent dewaxing liquid of pathological tissue, and constant temperature soaks 10 minutes at a temperature of 20 DEG C, is repeated
Immersion 1 time；The second transparent step is：Pathological tissue after dehydration of alcohol is put into the described transparent dewaxing of pathological tissue
In liquid, constant temperature soaks 3 minutes at a temperature of 23 DEG C, repeats immersion 2 times.
Embodiment 6
The transparent dewaxing liquid of pathological tissue of the present embodiment, including
Quadricyclane 94.9kg；
Trimethylolpropane 5kg；
Butyl ether dimethyl silicone polymer 0.1kg.
Above-mentioned height side chain saturated alkane, stabilizer and synergist are formed into the pathologic group in room temperature to blending at 60 DEG C
Knit transparent dewaxing liquid.
The transparent dewaxing liquid of pathological tissue of above-mentioned preparation is taken to carry out transparent for the first time, dewaxing to pathological tissue and saturating for the second time
Bright step：The first time transparent step is：Pathological tissue after dehydration of alcohol is put into described pathological tissue transparent
In dewaxing liquid, constant temperature soaks 45 minutes at a temperature of 35 DEG C, repeats immersion 1 time；The hydrodewaxing step is specially：By what is handled well
Pathological tissue paraffin section is put into the described transparent dewaxing liquid of pathological tissue, and constant temperature soaks 15 minutes at a temperature of 26 DEG C, weight
Soak 1 time again；Described second transparent:Pathological tissue after dehydration of alcohol is put into the described transparent dewaxing liquid of pathological tissue
In, constant temperature soaks 3 minutes at a temperature of 25 DEG C, repeats immersion 2 times.
Embodiment 7
The transparent dewaxing liquid of pathological tissue of the present embodiment, including：
Cis- 1- isopropyl-4-methyls hexamethylene 92.5kg；
Butyl epoxy stearate 5kg；
Dimethyl -3- hydroxypropyl methyls (siloxanes and polysiloxanes) 2.5kg.
Above-mentioned height side chain saturated alkane, stabilizer and synergist are formed into the pathologic group in room temperature to blending at 60 DEG C
Knit transparent dewaxing liquid.
The transparent dewaxing liquid of pathological tissue of above-mentioned preparation is taken to carry out transparent for the first time, dewaxing to pathological tissue and saturating for the second time
Bright step：Described transparent or second of transparent step is as follows, transparent for the first time:Pathological tissue after dehydration of alcohol is put
Enter in the described transparent dewaxing liquid of pathological tissue, constant temperature soaks 45 minutes at a temperature of 34 DEG C, repeat immersion 1 time；The dewaxing
Step is specially：The pathological tissue paraffin section handled well is put into the described transparent dewaxing liquid of pathological tissue, in 24 DEG C of temperature
The lower constant temperature of degree soaks 12 minutes, repeats immersion 2 times；Described second transparent：Pathological tissue after dehydration of alcohol is put into described
The transparent dewaxing liquid of pathological tissue in, at a temperature of 27 DEG C constant temperature soak 3 minutes, repeat immersion 2 times.
Embodiment 8
The transparent dewaxing liquid of pathological tissue of the present embodiment, including：
Trans- 1- isopropyl-4-methyls hexamethylene 97.5kg；
Butyl epoxy stearate 0.5kg；
Dimethyl -3- hydroxypropyl methyls (siloxanes and polysiloxanes) 2kg.
Above-mentioned height side chain saturated alkane, stabilizer and synergist are formed into the pathologic group in room temperature to blending at 60 DEG C
Knit transparent dewaxing liquid.
The transparent dewaxing liquid of pathological tissue of above-mentioned preparation is taken to carry out transparent for the first time, dewaxing to pathological tissue and saturating for the second time
Bright step：The first time transparent step is：Pathological tissue after dehydration of alcohol is put into described pathological tissue transparent
In dewaxing liquid, constant temperature soaks 45 minutes at a temperature of 32 DEG C, repeats immersion 1 time；The hydrodewaxing step is specially：By what is handled well
Pathological tissue paraffin section is put into the described transparent dewaxing liquid of pathological tissue, and constant temperature soaks 14 minutes at a temperature of 23 DEG C, weight
Soak 2 times again；Described second transparent：Pathological tissue after dehydration of alcohol is put into the described transparent dewaxing liquid of pathological tissue
In, constant temperature soaks 2 minutes at a temperature of 20 DEG C, repeats immersion 2 times.
Embodiment 9
The transparent dewaxing liquid of pathological tissue of the present embodiment, including：
Naphthenic oil 96kg；
Butyl epoxy stearate 3.5kg；
Butyl ether dimethyl silicone polymer 0.5kg.
Above-mentioned height side chain saturated alkane, stabilizer and synergist are formed into the pathologic group in room temperature to blending at 60 DEG C
Knit transparent dewaxing liquid.
The transparent dewaxing liquid of pathological tissue of above-mentioned preparation is taken to carry out transparent for the first time, dewaxing to pathological tissue and saturating for the second time
Bright step：The first time transparent step is：Pathological tissue after dehydration of alcohol is put into described pathological tissue transparent
In dewaxing liquid, constant temperature soaks 45 minutes at a temperature of 37 DEG C, repeats immersion 1 time；The hydrodewaxing step is specially：By what is handled well
Pathological tissue paraffin section is put into the described transparent dewaxing liquid of pathological tissue, and constant temperature soaks 12 minutes at a temperature of 24 DEG C, weight
Soak 2 times again；Described second transparent:Pathological tissue after dehydration of alcohol is put into the described transparent dewaxing liquid of pathological tissue
In, constant temperature soaks 3 minutes at a temperature of 28 DEG C, repeats immersion 2 times.
Embodiment 10
The transparent dewaxing liquid of pathological tissue of the present embodiment, including：
Naphthenic oil 50kg, quadricyclane 45kg；
Butyl epoxy stearate 1kg, epoxidized soybean oil 1kg；
Butyl ether dimethyl silicone polymer 0.5kg, dimethyl -3- hydroxypropyl methyl (siloxanes and polysiloxanes) 0.5kg.
Above-mentioned height side chain saturated alkane, stabilizer and synergist are formed into the pathologic group in room temperature to blending at 60 DEG C
Knit transparent dewaxing liquid.
The transparent dewaxing liquid of pathological tissue of above-mentioned preparation is taken to carry out transparent for the first time, dewaxing to pathological tissue and saturating for the second time
Bright step：The first time transparent step is：Pathological tissue after dehydration of alcohol is put into described pathological tissue transparent
In dewaxing liquid, constant temperature soaks 45 minutes at a temperature of 32 DEG C, repeats immersion 1 time;The hydrodewaxing step is specially：By what is handled well
Pathological tissue paraffin section is put into the described transparent dewaxing liquid of pathological tissue, and constant temperature soaks 13 minutes at a temperature of 28 DEG C, weight
Soak 2 times again；The second transparent step is：Pathological tissue after dehydration of alcohol is put into described pathological tissue transparent de-
In wax liquor, constant temperature soaks 3 minutes at a temperature of 25 DEG C, repeats immersion 2 times.
Embodiment 11
The transparent dewaxing liquid of pathological tissue of the present embodiment, including：
Cis- 1- isopropyl-4-methyls hexamethylene 80kg, quadricyclane 18kg；
Trimethylolpropane 0.5kg, epoxidized soybean oil 1kg；
Polyether-modified heptamethyltrisiloxane 0.8kg, dimethyl -3- hydroxypropyl methyl (siloxanes and polysiloxanes)
0.7kg。
Above-mentioned height side chain saturated alkane, stabilizer and synergist are formed into the pathologic group in room temperature to blending at 60 DEG C
Knit transparent dewaxing liquid.
The transparent dewaxing liquid of pathological tissue of above-mentioned preparation is taken to carry out transparent for the first time, dewaxing to pathological tissue and saturating for the second time
Bright step：The first time transparent step is：Pathological tissue after dehydration of alcohol is put into described pathological tissue transparent
In dewaxing liquid, constant temperature soaks 45 minutes at a temperature of 35 DEG C, repeats immersion 1 time；The hydrodewaxing step is specially：By what is handled well
Pathological tissue paraffin section is put into the described transparent dewaxing liquid of pathological tissue, and constant temperature soaks 13 minutes at a temperature of 23 DEG C, weight
Soak 2 times again；The second transparent step is：Pathological tissue after dehydration of alcohol is put into described pathological tissue transparent de-
In wax liquor, constant temperature soaks 3 minutes at a temperature of 25 DEG C, repeats immersion 2 times.
Embodiment 12
The transparent dewaxing liquid of pathological tissue of the present embodiment, including：
Naphthenic oil 30kg, trans- 1- isopropyl-4-methyls hexamethylene 63kg；
Trimethylolpropane 0.5kg, butyl epoxy stearate 2.5kg；
Polyether-modified heptamethyltrisiloxane 1kg, butyl ether dimethyl silicone polymer 1kg.
Above-mentioned height side chain saturated alkane, stabilizer and synergist are formed into the pathologic group in room temperature to blending at 60 DEG C
Knit transparent dewaxing liquid.
The transparent dewaxing liquid of pathological tissue of above-mentioned preparation is taken to carry out transparent, dewaxing and secondary transparent step to pathological tissue
Suddenly：The first time transparent step is：Pathological tissue after dehydration of alcohol is put into the described transparent dewaxing liquid of pathological tissue
In, constant temperature soaks 45 minutes at a temperature of 32 DEG C, repeats immersion 1 time；The hydrodewaxing step is specifically, by the pathologic group handled well
Knit paraffin section to be put into the described transparent dewaxing liquid of pathological tissue, constant temperature soaks 15 minutes at a temperature of 24 DEG C, repeat immersion 2
It is secondary；The second transparent step is:Pathological tissue after dehydration of alcohol is put into the described transparent dewaxing liquid of pathological tissue,
Constant temperature soaks 3 minutes at a temperature of 27 DEG C, repeats immersion 2 times.
Obviously, above-described embodiment is only intended to clearly illustrate example, and the not restriction to embodiment.It is right
For those of ordinary skill in the art, can also make on the basis of the above description it is other it is various forms of change or
Change.There is no necessity and possibility to exhaust all the enbodiments.And the obvious change thus extended out or
Among changing still in the protection domain of the invention.
Claims (10)
1. a kind of transparent dewaxing liquid of pathological tissue, it is characterised in that include the component of following parts by weight：
Height side chain saturated alkane 92.5-98 parts by weight；
Stabilizer 0.5-5 parts by weight；
Synergist 0.1-2.5 parts by weight.
2. the transparent dewaxing liquid of pathological tissue according to claim 1, it is characterised in that the transparent dewaxing liquid of pathological tissue
Include the component of following parts by weight：
Height side chain saturated alkane is 95-97 parts by weight；
Stabilizer 2-3 parts by weight；
Synergist 1-2 parts by weight.
3. the transparent dewaxing liquid of pathological tissue according to claim 1 or 2, it is characterised in that：
The height side chain saturated alkane is naphthenic oil, quadricyclane, cis- 1- isopropyl-4-methyls hexamethylene, trans- 1- isopropyls
One or more of mixtures in base -4- hexahydrotoluenes.
4. the transparent dewaxing liquid of pathological tissue according to claim 1 or 2, it is characterised in that：
Described stabilizer is one or more of mixing in epoxidized soybean oil, trimethylolpropane, butyl epoxy stearate
Thing.
5. the transparent dewaxing liquid of pathological tissue according to claim 1 or 2, it is characterised in that：
Described synergist is polyether-modified heptamethyltrisiloxane, butyl ether dimethyl silicone polymer, dimethyl -3- hydroxypropyls
One or more of mixtures in methyl (siloxanes and polysiloxanes).
6. the transparent dewaxing liquid of pathological tissue according to claim 3, it is characterised in that the transparent dewaxing liquid of pathological tissue
Constitute and be：
The parts by weight of naphthenic oil 98, the parts by weight of epoxidized soybean oil 0.5, the parts by weight of polyether-modified heptamethyltrisiloxane 1.5；Or
The parts by weight of quadricyclane 94.9, the parts by weight of trimethylolpropane 5, the parts by weight of butyl ether dimethyl silicone polymer 0.1；Or
The cis- parts by weight of 1- isopropyl-4-methyls hexamethylene 92.5, the parts by weight of butyl epoxy stearate 5, dimethyl -3- hydroxypropyls
Methyl (siloxanes and polysiloxanes) 2.5 parts by weight；Or
The trans- parts by weight of 1- isopropyl-4-methyls hexamethylene 97.5, the parts by weight of butyl epoxy stearate 0.5, dimethyl -3- hydroxypropyls
Ylmethyl (siloxanes and polysiloxanes) 2 parts by weight；Or
The parts by weight of naphthenic oil 96, the parts by weight of butyl epoxy stearate 3.5, the parts by weight of butyl ether dimethyl silicone polymer 0.5.
7. a kind of method for preparing the transparent dewaxing liquid of pathological tissue as described in claim 1-6 is any, it is characterised in that including by
The height side chain saturated alkane, stabilizer and synergist are taken according to selected parts by weight, in room temperature to 60 DEG C of steps reconciled
Suddenly.
8. the pathology that any transparent dewaxing liquids of pathological tissue of claim 1-6 or claim 7 methods described are prepared
Application of the transparency of organization dewaxing liquid in pathological tissue paraffin section field is made.
9. a kind of preparation method of pathological tissue paraffin section, it is characterised in that including with any described diseases of claim 1-6
Manage the step of transparency of organization dewaxing liquid carries out transparent for the first time, dewaxing and second of transparent operation to the pathological tissue.
10. the preparation method of pathological tissue paraffin section according to claim 9, it is characterised in that：
The hydrodewaxing step is specially：The pathological tissue paraffin section handled well is put into the described transparent dewaxing liquid of pathological tissue
In, constant temperature soaks 10-15 minutes at a temperature of 20-28 DEG C, repeats immersion 2 times；
Described first time, transparent step was specially：Pathological tissue after dehydration of alcohol is put into described pathological tissue transparent de-
In wax liquor, constant temperature soaks 40-50 minutes at a temperature of 32-37 DEG C, repeats immersion 1 time；
Described second transparent step be specially：Pathological tissue after dehydration of alcohol is put into described pathological tissue transparent de-
In wax liquor, constant temperature soaks 2-5 minutes at a temperature of 20-28 DEG C, repeats immersion 2 times.
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| CN104155160A (en) * | 2014-08-12 | 2014-11-19 | 陆可望 | Tissue dewaxing transparent agent free of benzene |
| CN106370491B (en) * | 2016-08-30 | 2019-03-08 | 河南新大阳生物技术有限公司 | Environment friendly transparent dewaxing liquid and preparation method thereof and its application in pathological section preparation |
| CN109470534B (en) * | 2018-11-12 | 2021-05-25 | 山东骏腾医疗科技有限公司 | Pathological tissue transparent dewaxing liquid and preparation method thereof |
| CN109827821B (en) * | 2019-03-14 | 2021-11-09 | 武汉原谷生物科技有限责任公司 | Non-xylene dewaxing repairing agent for paraffin section and preparation method thereof |
| CN110146354B (en) * | 2019-05-14 | 2021-11-23 | 东部战区总医院秦淮医疗区 | Method for dissolving paraffin by modified trimethylolpropane fatty acid ester |
| CN110243647A (en) * | 2019-07-03 | 2019-09-17 | 安徽雷根生物技术有限公司 | A method of the dewaxing of no benzene is transparent |
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| CN102183387A (en) * | 2011-01-21 | 2011-09-14 | 哈尔滨格林标本技术开发有限公司 | Benzene-free environment-friendly tissue or slice clearing liquid |
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| CN102183387A (en) * | 2011-01-21 | 2011-09-14 | 哈尔滨格林标本技术开发有限公司 | Benzene-free environment-friendly tissue or slice clearing liquid |
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