CN103055038A - Pure Chinese medicinal composition for treating bee chalkbrood disease and preparation method thereof - Google Patents

Abstract

The invention relates to a pure Chinese medicinal composition for treating bee chalkbrood disease. The pure Chinese medicinal composition is prepared by mixing and extracting the following components in part by weight: 25 to 35 parts of dried alum, 25 to 45 parts of borneol, 15 to 35 parts of safflower, 15 to 35 parts of coptis root, 15 to 35 parts of snake gall, 15 to 35 parts of cortex pseudolaricis, 15 to 35 parts of liquoric root, 15 to 35 parts of wrinkled gianthyssop herb, 25 to 35 parts of blackberry lily and 25 to 35 parts of Chinese wolfberry root-bark. The pure Chinese medicinal composition has the advantages that the composition is reasonable in formula and easy to prepare, does not have any toxic or side effects, treats both symptoms and root causes, and has a very obvious effect; medicine residues and medicine resistance are avoided; and the clinical results prove that the pure Chinese medicinal composition has a special effect of treating bee chalkbrood disease.

Description

A kind of pure Chinese medicine composition for the treatment of ascospherosis of bee and preparation method thereof

Technical field

The present invention relates to pure Chinese medicine composition for the treatment of ascospherosis of bee and preparation method thereof, belong to the animal field of medicaments, particularly a kind of pure Chinese medicine composition that is specifically designed to the treatment ascospherosis of bee and preparation method thereof.

Background technology

Ascospherosis of bee has another name called the calcium carbonate disease, is a kind of fungal infectious disease that is caused bee larvae death by the Ascosphaera apis parasitism, and the multiple the spring or early summer of being born in particularly easily occurs under overcast and rainy moist environmental condition.Ascosphaera apis is a kind of fungus, only attacks bee larvae.Die of illness larva and contaminated feedstuff, Nidus Vespaes etc. are the primary disease sources of infection.Mainly propagate by sporangiospore and ascospore.Bee larvae eats the feedstuff of pollution, and spore is sprouted at enteral, grows mycelia and penetrable intestinal wall, and a large amount of mycelia make enterorrhexis behind the larva and dead, and forms Sporangium at dead polypide table.After the larva death, just be pale asphyxia, rear grey or black.Become open-textured Chalk shape thing after the larva corpse is withered, body surface is covered with white hypha.

This disease spreads all over more than 100 countries of five continents, is widely distributed.At present, treat this disease the most frequently used be various antifungal drugs, though receive certain effect, because its toxic and side effects by force, easily produces the problems such as drug resistance and drug residue, clinical practice is very limited or bans use of.

Therefore researching and developing a kind of specific medicament for the treatment of ascospherosis of bee is one of this field scientific research technical staff new problem anxious to be resolved.

Summary of the invention

The object of the invention is to overcome above-mentioned weak point, provide that a kind of reasonable recipe, preparation are simple, successful, effectively treat the pure Chinese medicine composition and preparation method thereof of ascospherosis of bee.

Implementation of the present invention is as follows for achieving the above object: a kind of pure Chinese medicine composition for the treatment of ascospherosis of bee, mixed extraction is formulated by weight by following component: dried Alumen 25-35 part, Borneolum Syntheticum 25-45 part, Flos Carthami 15-35 part, Rhizoma Coptidis 15-35 part, the sub-15-35 part of Fel Serpentis, Cortex Pseudolaricis 15-35 part, Radix Glycyrrhizae 15-35 part, Herba Pogostemonis 15-35 part, Rhizoma Belamcandae 25-35 part, Cortex Lycii 25-35 part.

Described various component optimum ratio amount is: 30 parts of dried Alumens, 35 parts of Borneolum Syntheticums, 25 parts on Flos Carthami, 25 parts of Rhizoma Coptidis, 25 parts of Fel Serpentis, 25 parts of Cortex Pseudolaricis, 25 parts in Radix Glycyrrhizae, 25 parts of Herba Pogostemoniss, 30 parts of Rhizoma Belamcandae, 30 parts of Cortex Lycii.

A kind of preparation method for the treatment of the pure Chinese medicine composition of ascospherosis of bee, carry out as follows:

(1) the various compositions in the prescription (except dried Alumen, Borneolum Syntheticum) is pulverized respectively rear 50 mesh sieves of crossing;

(2) take by weighing by weight Flos Carthami 15-35 part, Rhizoma Coptidis 15-35 part, the sub-15-35 part of Fel Serpentis, Cortex Pseudolaricis 15-35 part, Radix Glycyrrhizae 15-35 part, Herba Pogostemonis 15-35 part, Rhizoma Belamcandae 25-35 part, Cortex Lycii 25-35 part, mix 5-10 minute to even;

(3) add by weight 8 times of water gaging warm macerating 1 hour (60 ℃～80 ℃) of medical material total amount described in (2), then decocted 60 minutes, filter; The gained filtering residue adds 4 times of decoctings again and boiled 30 minutes, filters, and merging filtrate adds dried Alumen 25-35 part, Borneolum Syntheticum 25-45 part, is evaporated to relative density 1.1, embedding, and sterilization, and get final product.

The medicinal property of each component is as follows:

Dried Alumen: cold, acid, puckery, return lung, spleen, large intestine, Liver Channel, have the function of heat clearing away expectorant, hemostasis antidiarrheal, dampness removing to stop itchin, detoxifcation parasite killing; Cure mainly incessant chronic diarrhea, heavily wait after having blood in stool, let out dysentery.

Borneolum Syntheticum: hot, bitter, cold, GUIXIN, spleen, lung meridian have the function of sensible refreshment, heat-clearing and fire-dispersing, reducing swelling and alleviating pain; Cure mainly coma convulsions, laryngopharynx swelling and pain, heat in the heart tongue boil, conjunctival congestion cataracta, sore swollen toxin etc.

Flos Carthami: hot, warm, return liver, heart channel, have invigorate blood circulation, the function of dissipating blood stasis, pain relieving; Cure mainly congestion pain, placenta retention, lochiorrhea etc.

Rhizoma Coptidis: bitter, cold, return liver, the heart, spleen, stomach, gallbladder, large intestine channel, have the function of heat clearing and damp drying, eliminating fire and detoxication; Cure mainly damp-heat dysentery, hyperactivity of heart-fire, excessiveness of stomach-fire, dampness-heat in the liver and gallbladder, conjunctival congestion and swelling pain, fire-toxin carbuncle sore etc.

Fructus Cnidii: hot, bitter, warm, return kidney channel, have that warming the kidney to invigorate YANG, dampness dispel the wind, the function of killing parasites for relieving itching; Cure mainly impotence due to deficiency of the kidney, eczema, pruritus etc.

Cortex Pseudolaricis: hot, warm, return lung meridian, have the function of killing parasites for relieving itching; Cure mainly eczema, pruritus, tinea etc.

Radix Glycyrrhizae: sweet, flat, return lung, the heart, spleen channel, have invigorating the spleen and replenishing QI, expelling phlegm for arresting cough and middle emergency, detoxifcation, coordinating the actions of various ingredients in a prescription, cushion toxicity, strong function; Cure mainly weakness of the spleen and stomach, cough with asthma, poisoning etc.

Herba Pogostemonis: hot, tepor, return lung, spleen, stomach warp, have the function of delivering expelling summer-heat, removing dampness by means of aromatics and middle preventing or arresting vomiting; Cure mainly that the summer Sunstroke is wet, diarrhea due to summer heat and dampness, regurgitation vomiting, tripe distention and fullness in the abdomen etc.

Rhizoma Belamcandae: bitter, cold, return lung meridian, have the function of heat-clearing and toxic substances removing, expectorant, sore-throat relieving; Cure mainly cough due to lung-heat, laryngopharynx swelling and pain, accumulation and obstruction of sputum etc.

Cortex Lycii: sweet, cold, return liver, lung, kidney channel, have that removing heat from blood is brought down a fever, the function of lung heat clearing pathogenic fire reducing; Cure mainly YinXuXueRe, lung-heat cough with asthma etc.

Above all flavor Chinese medicine for pathogenic microorganism and the clinical symptoms of ascospherosis of bee, is brought into play the effect for the treatment of both the principal and secondary aspects of a disease according to the theoretical compatible combination of Chinese veterinarian.

The invention has the beneficial effects as follows: reasonable recipe, preparation is simple, and cost is low, draws materials wide, drug residue free, has no drug resistance, has no side effect, and Chinese medicine extraction is sprayed, the curative effect highly significant.The present invention optimizes this Ten Herbs according to Chinese veterinarian's traditional theory, and makes them form pure Chinese medicine according to optimum ratio, and clinical practice proves that the treatment ascospherosis of bee is had specially good effect.

The specific embodiment

Below in conjunction with preferred embodiment, to details are as follows according to the specific embodiment provided by the invention:

Embodiment 1

A kind of pure Chinese medicine composition for the treatment of ascospherosis of bee and preparation method thereof: carry out as follows: (every part by 10g)

(1) the various compositions in the prescription (except dried Alumen, Borneolum Syntheticum) is pulverized respectively rear 50 mesh sieves of crossing;

(2) take by weighing by weight 150 parts on Flos Carthami, 150 parts of Rhizoma Coptidis, 150 parts of Fel Serpentis, 150 parts of Cortex Pseudolaricis, 150 parts in Radix Glycyrrhizae, 150 parts of Herba Pogostemoniss, 250 parts of Rhizoma Belamcandae, 250 parts of Cortex Lycii, mix 5-10 minute to even;

(3) add by weight 8 times of water gaging warm macerating 1 hour (60 ℃～80 ℃) of medical material total amount described in (2), then decocted 60 minutes, filter; The gained filtering residue adds 4 times of decoctings again and boiled 30 minutes, filters, and merging filtrate adds 250 parts of dried Alumens, 250 parts of Borneolum Syntheticums, is evaporated to relative density 1.1, embedding, and sterilization, and get final product.

Embodiment 2

A kind of pure Chinese medicine composition for the treatment of ascospherosis of bee and preparation method thereof: carry out as follows: (every part by 10g)

(1) the various compositions in the prescription (except dried Alumen, Borneolum Syntheticum) is pulverized respectively rear 50 mesh sieves of crossing;

(2) take by weighing by weight 250 parts on Flos Carthami, 250 parts of Rhizoma Coptidis, 250 parts of Fel Serpentis, 250 parts of Cortex Pseudolaricis, 250 parts in Radix Glycyrrhizae, 250 parts of Herba Pogostemoniss, 300 parts of Rhizoma Belamcandae, 300 parts of Cortex Lycii, mix 5-10 minute to even;

(3) add by weight 8 times of water gaging warm macerating 1 hour (60 ℃～80 ℃) of medical material total amount described in (2), then decocted 60 minutes, filter; The gained filtering residue adds 4 times of decoctings again and boiled 30 minutes, filters, and merging filtrate adds 300 parts of dried Alumens, 350 parts of Borneolum Syntheticums, is evaporated to relative density 1.1, embedding, and sterilization, and get final product.

Embodiment 3

A kind of pure Chinese medicine composition for the treatment of ascospherosis of bee and preparation method thereof: carry out as follows: (every part by 10g)

(1) the various compositions in the prescription (except dried Alumen, Borneolum Syntheticum) is pulverized respectively rear 50 mesh sieves of crossing;

(2) take by weighing by weight 350 parts on Flos Carthami, 350 parts of Rhizoma Coptidis, 350 parts of Fel Serpentis, 350 parts of Cortex Pseudolaricis, 350 parts in Radix Glycyrrhizae, 350 parts of Herba Pogostemoniss, 350 parts of Rhizoma Belamcandae, 350 parts of Cortex Lycii, mix 5-10 minute to even;

(3) add by weight 8 times of water gaging warm macerating 1 hour (60 ℃～80 ℃) of medical material total amount described in (2), then decocted 60 minutes, filter; The gained filtering residue adds 4 times of decoctings again and boiled 30 minutes, filters, and merging filtrate adds 350 parts of dried Alumens, 450 parts of Borneolum Syntheticums, is evaporated to relative density 1.1, embedding, and sterilization, and get final product.

Clinical trial:

It is the ill Apis of ascospherosis of bee that the clinical experiment animal is selected Laboratory Diagnosed.The isolation of ill honeybee is raised separately, be divided at random 3 groups, every group of 3 casees totally 15 spleen honeybees, 3 groups of feeding environments are identical, and experimental period was carried out 5 days altogether; The blank group is left intact, and the medicine matched group uses treatment with institute's control drug by its description; Present composition group in 3: 2 ratio mix homogeneously, is sprayed spleen every day 1 time, continuous 4 days with product of the present invention (embodiment of the invention 1 prepared compositions) and distilled water.Computational methods: mortality rate=dead animal number/experimental animal number * 100%, cure rate=healing number of animals/experimental animal number * 100%.Result's (testing the 4th day) when following table is the experiment end.

Table is the applicable cases contrast of the present invention and other medicines treatment ascospherosis of bee:

Numbering	Group	Mortality rate %	Cure rate %	Symptom rate % is arranged during end
					I	The blank group	21	54	25
II	Griseofulvin spray group	10	80	10
					III	Present composition group	6	87	7

This clinical effectiveness statistical analysis, present composition treatment group is being compared significant difference (P＜0.05) with griseofulvin spray group aspect the cure rate.

Safety testing:

Choose 1 case 5 spleen of healthy Apis, 5 times according to the embodiment of the invention 1 made compositions recommended dose are sprayed 4 times continuously, during the medication, healthy Apis is without any the performance of bad or Deviant Behavior, and to compare difference not remarkable with blank group (being not administration of health group).

Above-mentioned detailed description of pure Chinese medicine composition of this treatment ascospherosis of bee and preparation method thereof being carried out with reference to embodiment; illustrative rather than determinate; can list several embodiment according to institute's limited range; therefore in the variation and the modification that do not break away under the general plotting of the present invention, should belong within protection scope of the present invention.

Claims (3)

1. pure Chinese medicine composition for the treatment of ascospherosis of bee, it is characterized in that by following component by weight mixed extraction form: dried Alumen 25-35 part, Borneolum Syntheticum 25-45 part, Flos Carthami 15-35 part, Rhizoma Coptidis 15-35 part, the sub-15-35 part of Fel Serpentis, Cortex Pseudolaricis 15-35 part, Radix Glycyrrhizae 15-35 part, Herba Pogostemonis 15-35 part, Rhizoma Belamcandae 25-35 part, Cortex Lycii 25-35 part.

2. the pure Chinese medicine composition for the treatment of ascospherosis of bee according to claim 1, it is characterized in that by following component by weight mixed extraction form: 30 parts of dried Alumens, 35 parts of Borneolum Syntheticums, 25 parts on Flos Carthami, 25 parts of Rhizoma Coptidis, 25 parts of Fel Serpentis, 25 parts of Cortex Pseudolaricis, 25 parts in Radix Glycyrrhizae, 25 parts of Herba Pogostemoniss, 30 parts of Rhizoma Belamcandae, 30 parts of Cortex Lycii.

3. the preparation method of the pure Chinese medicine composition of a treatment ascospherosis of bee according to claim 1 and 2 is characterized in that carrying out as follows:

(1) the various compositions in the prescription (except dried Alumen, Borneolum Syntheticum) is pulverized respectively rear 50 mesh sieves of crossing;

(2) take by weighing by weight Flos Carthami 15-35 part, Rhizoma Coptidis 15-35 part, the sub-15-35 part of Fel Serpentis, Cortex Pseudolaricis 15-35 part, Radix Glycyrrhizae 15-35 part, Herba Pogostemonis 15-35 part, Rhizoma Belamcandae 25-35 part, Cortex Lycii 25-35 part, mix 5-10 minute to even;

(3) add by weight 8 times of water gaging warm macerating 1 hour (60 ℃～80 ℃) of medical material total amount described in (2), then decocted 60 minutes, filter; The gained filtering residue adds 4 times of decoctings again and boiled 30 minutes, filters, and merging filtrate adds dried Alumen 25-35 part, Borneolum Syntheticum 25-45 part, is evaporated to relative density 1.1, embedding, and sterilization, and get final product.