CN103735628B - A kind of medicine for the treatment of ascospherosis of bee and preparation method thereof - Google Patents
A kind of medicine for the treatment of ascospherosis of bee and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a kind of medicine for the treatment of ascospherosis of bee and preparation method thereof, get the bulk drug of following weight: yellow palpus dish 1-10g, capsule of weeping forsythia 1-10 part, inular flower 1-10 part, clam skin 1-10 part, dietary alkali 20-45 part and salt 20-45 part; Particularly raw material proportioning of the present invention yellow must 5 parts of dishes, when 40 parts of 5 parts of the capsules of weeping forsythia, 5 parts of inular flowers, 5 parts of clam skins, 40 parts of dietary alkalis and salt, its result for the treatment of the best to ascospherosis of bee.
Description
Technical field
The present invention relates to a kind of medicine for the treatment of ascospherosis of bee and preparation method thereof, belong to technical field of pharmaceuticals.
Background technology
Chalk disease is the infectiousness nosomycosis of a kind of bee larva, is mainly distributed in the areas such as Europe, North America and China, inState was reported first in 1991, popular in this disease apis mellifera in China at present, harm especially severe.
Chalk disease occur in the humiture depending on to a great extent at that time, have obvious seasonality, generally thisSick being popular in spring and early summer, particularly, overcast and rainy moist, variations in temperature easily produces under weather conditions frequently more. In this sectionTime, bee colony many places are breeding period, and on honeycomb, subloop is larger, and the chance of catching a cold in honeycomb edge is larger, and the incidence of disease is just high. ?In bee colony, the corpse of ill larva and contaminated feed and honeycomb are the main sources of transmission.
Sporangium propagation and the optimum temperature forming are 30 DEG C of left and right, when honeycomb temperature drops to 30 DEG C from 35 DEG C, and larvaEasy infection. Therefore in the time of bee colony amount reproduction, due to the bad or lactation honeybee deficiency of insulation, the easiest while causing the interior larva of nest to catch a coldOccur. Occur annual 4～October, and be peak period 4～June. Moist, excessive swarmming, the outmoded mouldy pollen of feeding, appliedMany antibiotic, to change in honeybee enteron aisle microbiota, bee colony weak etc., all can bring out chalk disease.
The larva of general 4 ages in days very easily infects, and drone larvae easily infects compared with worker bee, and infected larva occurs that polypide is swollenSwollen and grow white fine hair and be full of whole lair, polypide presents hexagon shape, then fold, hardening, turn white, head blackout,Room lid is usually stung out by worker bee. A large amount of hard polypides that turn white of corpse shape are hauled out beehive doorway.
Early stage, sick worm becomes white larva without a head, and body colour is similar to healthy larva, and body surface not yet forms mycelia; InPhase, larva is soft expands, and the outside of belly covers with white hypha; In the later stage, whole larva body is covered with white hypha, polypide atrophy and byGradual change is hard, like chalk shape. Two kinds of dead worm corpse adularescent, black.
Worker bee is by polypide by hauling out to nest ground in front of the door and bottom board in lair, worker bee and drone larvae all can be feltDisease, drone larvae is particularly serious. Large larval stage susceptible, lair cover worker bee breaks by the teeth, chooses out rear visible dead larva.
Infect the larva of ascus coccus, non-evident sympton performance in first 3 days, most larvas were death in the 5th day. Honeybee childrenIn polypide, pathology is divided into 6 stages.
1. spore resting stage (3～48 hours): can find sporangiocyst in intestines this period in larva, and spore ball scattersIn food, spore is bonded in and on spore ball, is inactive state.
2. the spore germination phase (3～72 hours): spore, in start sprouting in enteron aisle, expands when spore germination for ball-type, soAfter stretch out germ tube, now midgut tissue is infected, and still belongs to normal.
3. mycelia proliferation period (24～72 hours): after spore germination, form mycelia in food lumps in middle intestines, and have sporeSon produces, and now midgut tissue still occurs without pathology, inoculates 48～72 hours, and mycelia penetrates funnel by enteron aisle inwall and invadesDermal cell.
4. penetrate the intestines wall phase (72～96 hours): inoculate after 72 hours, mycelial growth is vigorous, and has hypha body to penetrateIntestines wall, middle intestines outer wall dermal cell has perforation, and part cell is damaged, and after 4～5 days, middle intestines are damaged and find whole group bacteriumSilk passes from Malpighian tube.
5. body cavity proliferation period (72～120 hours): seedling silk penetrates after intestines wall or Malpighian tube is constantly bred in body cavity,Cause fat-body, tracheae and muscle generation pathology.
6. penetrate the body wall phase (96～120 hours): mycelia is amount reproduction in body cavity, infects body wall simultaneously, and body surface fillsFull mycelia, male and female mycelia in vitro mating produces sporangiocyst. Generation and the humiture of chalk disease are in close relations, when temperature in nest declinesTo 30 DEG C, when relative humidity 80% is above, be suitable for ascospore growth, spring and summer, rainy wet season was easily popular.
The treatment of ascospherosis of bee generally adopts the method for chemical agent to treat, such as adopt 0.1%～0.2% newGeramine solution spray honeybee, spray spleen, spray honeybee with moldin or other antimicrobial, adopts chemical agent to treat, treatment effectFruit is bad, the little bactericidal effect that do not have of dosage, and dosage is large easily large to the injury of bee colony, and the honey quality that bee colony is madeThere is pollution, in honey, contain the residual of medicine.
Also there is the Chinese herbal medicine proved recipe of employing to treat, feed as after adopting Chinese medicine to be boiled into blend syrup after concoction,Although adopt Chinese medicine preparation pollution-free to honey; But adopt the drug effect of this kind of mode medicine undesirable, and the processing of medicine is numerousTrivial, making step complexity, it is very inconvenient to use, because putting in a suitable place to breed of honeybee is all in some comparatively remote regions, the taking of medicineBand and processing are very inconvenient.
Summary of the invention
The problem to be solved in the present invention is for above problem, and a kind of medicine and preparation thereof for the treatment of ascospherosis of bee is providedMethod, the medicine of this treatment ascospherosis of bee has instant effect, to the free of contamination advantage of honey, the health of honeybee is done without pairWith.
For overcoming the above problems, technical scheme of the present invention is:
Treat a medicine for ascospherosis of bee, get the bulk drug of following weight: yellow must dish 1-10g, capsule of weeping forsythia 1-10 part, revolveSpend again 1-10 part, clam skin 1-10 part, dietary alkali 20-45 part and salt 20-45 part.
Below the further improvement to technique scheme:
Treat a medicine for ascospherosis of bee, get the bulk drug of following weight: yellow 5 parts of dishes of palpus, 5 parts of the capsules of weeping forsythia, inular flower 540 parts of part, 5 parts of clam skins, 40 parts of dietary alkalis and salt.
A preparation method who treats the medicine of ascospherosis of bee, comprises the following steps:
1), get the raw materials ready according to above-mentioned raw materials:
2), by Huang must dish airing 10-15 days, until dry completely, be then ground into smalls, obtain smalls A;
3), by the capsule of weeping forsythia clean dried and crushed become smalls, obtain smalls B;
4), by inular flower clean dried and crushed become smalls, obtain smalls C;
5), clam skin is ground into smalls, obtain smalls D;
6), above-mentioned smalls A, smalls B, smalls C, smalls D, dietary alkali and salt are fully mixed and both obtain medicinal powder.
In step 5), clam skin high-temperature heating, to lime shape, is then pulverized.
Through testing in raw material proportioning of the present invention yellow palpus dish 1-10 part, capsule of weeping forsythia 1-10 part, inular flower 1-10 part, clamSkin 1-10 part, dietary alkali 20-45 part and salt 20-45 part, the effect for the treatment of ascospherosis of bee is better than other weight proportions far away.
Particularly raw material proportioning of the present invention is at yellow 5 parts of dishes of palpus, 5 parts of the capsules of weeping forsythia, 5 parts of inular flowers, 5 parts of clam skins, dietary alkaliWhen 40 parts of 40 parts and salt, its result for the treatment of the best to ascospherosis of bee.
Use after this medicine, in two days, the infection of larva has remarkable alleviation, and dead larva continues to be hauled out honeycomb, in four daysThe infection of larva is substantially controlled, does not have dead larva to continue to be hauled out honeycomb, and in beehive, larva is in good condition, infects completelyControlled, do not have new infection to occur, do not have dead larva to continue to be hauled out honeycomb, open beehive, larva state in beehiveWell, and honey quality good pollution-free.
Below in conjunction with detailed description of the invention, technical scheme of the present invention is described further.
Detailed description of the invention
Embodiment 1, a kind of medicine for the treatment of ascospherosis of bee, yellow palpus dish 5g, capsule of weeping forsythia 5g, inular flower 5g, clam skin 5g, foodWith alkali 40g and salt 40g.
A preparation method who treats the medicine of ascospherosis of bee, comprises the following steps:
1), get the raw materials ready according to above-mentioned raw materials:
2), by Huang must dish airing 10-15 days, until dry completely, be then ground into smalls, obtain smalls A;
3), by the capsule of weeping forsythia clean dried and crushed become smalls, obtain smalls B;
4), by inular flower clean dried and crushed become smalls, obtain smalls C;
5), clam skin high temperature is ground into smalls, obtain smalls D;
6), above-mentioned smalls A, smalls B, smalls C, smalls D, dietary alkali and salt are fully mixed and both obtain medicinal powder.
In step 5), clam skin high-temperature heating, to lime shape, is then pulverized.
Embodiment 2, a kind of medicine for the treatment of ascospherosis of bee, get the bulk drug of following weight: yellow palpus dish 1g, capsule of weeping forsythia 1g,Inular flower 1g, clam skin 1g, dietary alkali 20g and salt 20g.
A preparation method who treats the medicine of ascospherosis of bee, comprises the following steps:
1), get the raw materials ready according to above-mentioned raw materials:
2), by Huang must dish airing 10-15 days, until dry completely, be then ground into smalls, obtain smalls A;
3), by the capsule of weeping forsythia clean dried and crushed become smalls, obtain smalls B;
4), by inular flower clean dried and crushed become smalls, obtain smalls C;
5), clam skin high temperature is ground into smalls, obtain smalls D;
6), above-mentioned smalls A, smalls B, smalls C, smalls D, dietary alkali and salt are fully mixed and both obtain medicinal powder.
In step 5), clam skin high-temperature heating, to lime shape, is then pulverized.
Embodiment 3, a kind of medicine for the treatment of ascospherosis of bee, get the bulk drug of following weight: yellow palpus dish 10g, the capsule of weeping forsythia10g, inular flower 10g, clam skin 10g, dietary alkali 45g and salt 45g.
A preparation method who treats the medicine of ascospherosis of bee, comprises the following steps:
1), get the raw materials ready according to above-mentioned raw materials:
2), by Huang must dish airing 10-15 days, until dry completely, be then ground into smalls, obtain smalls A;
3), by the capsule of weeping forsythia clean dried and crushed become smalls, obtain smalls B;
4), by inular flower clean dried and crushed become smalls, obtain smalls C;
5), clam skin high temperature is ground into smalls, obtain smalls D;
6), above-mentioned smalls A, smalls B, smalls C, smalls D, dietary alkali and salt are fully mixed and both obtain medicinal powder.
When use, the medicinal powder of gained is evenly scattered in beehive, 6 sub-spleens of a beehive, spill beehive by medicinal powderOn Feng road, a beehive 30g left and right, spills once for three days.
In step 5), clam skin high-temperature heating, to lime shape, is then pulverized.
In above-mentioned three embodiment, can use commercially available common dietary alkali, the kite that adopts Weifang Pure Alkali Factory, Shandong Prov. to produceAll board dietary alkalis, main component sodium carbonate, the result of use of medicine is best.
Get 12 groups of beehives, 6 sub-spleens of a beehive, carry out contrast experiment to technical scheme of the present invention and conventional method.
One group adopts moldin to feed, and every liter of 50% syrup adds 200 milligrams of this medicines, every 3 days 1 time, is used in conjunction 5 times.
Two groups adopt the 75% alcoholic solution portion nine parts of wiring solution-formings that add water by weight, directly spray honeycomb.
Three groups with 3 grams of Ofloxacins, then add 1000 milliliters, the syrup water of 1:1, mix thoroughly, and every day, every group feeding at dusk fed 100～150 milliliters, every day 1 time, even feeding 7 days was 1 course for the treatment of, and can substantially cure 1～2 course for the treatment of.
Four groups with 20 milliliters of geramine liquid, the next day spray spleen 1 time, connect that to spray be for 4-5 time a course for the treatment of.
Five groups adopt 60 grams of gold and silver, 60 grams of the capsules of weeping forsythia, 20 grams of 40 grams of ， Chuan Gong of dandelion, 12 grams, Radix Glycyrrhizae, 60 grams of mother chrysanthemums, carFirst careless 60 grams, add water 2 kilograms, decoct to 1 kilogram for subsequent use, for spray spleen or add syrup feed honeybee, 1 day 1 time is for 3 times a course for the treatment of,Treat 3 courses for the treatment of.
Six groups adopt 40 grams of 20 grams of gold and silver, 40 grams of the capsules of weeping forsythia, inular flower to add water 1000 milliliters, decoct approximately 300 milliliters with low baking temperatureTime pour out liquid, then add water 200 milliliters to decoct after 5 minutes and pour out liquid, with primary liquid mixing for standby use, to ill honeybeeGroup is sprayed spleen 1 time every day, for three days on end.
Seven groups adopt 20 grams of gold and silver, 40 grams of the capsules of weeping forsythia, inular flower 40 to wear into fine powder, and ill bee colony is sprayed to every next honeybeeCase 30g left and right, sprays 1 time for 3 days.
Eight groups of saltings on Xiang Dihuofeng road, a beehive 30g left and right, sprays once for 3 days.
Get for nine groups sodium bicarbonate is spread in honeybee road, a beehive 30g left and right, sprays once for 3 days.
Ten groups of medicinal powder that adopt the embodiment of the present invention 1 to make, a beehive 30g left and right, sprays once for 3 days.
11 groups of medicinal powder that adopt the embodiment of the present invention 2 to make, a beehive 30g left and right, sprays once for 3 days.
12 groups of medicinal powder that adopt the embodiment of the present invention 3 to make, a beehive 30g left and right, sprays once for 3 days.
Carry out contrast experiment to above-mentioned 12 groups, being chosen in of 12 groups of bee colonies statistically has comparativity, its experimentThe results are shown in following table one.
Through experiment, embodiments of the invention 1 are optimum embodiment; Through testing in raw material proportioning of the present invention at yellow palpusWhen dish 1-10g, capsule of weeping forsythia 1-10g, inular flower 1-10g, clam skin 1-10g, dietary alkali 20-40g and salt 20-40g, treatment honeybeeThe effect of chalk disease is better than other weight proportions far away.
Particularly raw material proportioning of the present invention yellow must dish 5g, capsule of weeping forsythia 5g, inular flower 5g, clam skin 5g, dietary alkali 40g andWhen salt 40g, its result for the treatment of the best to ascospherosis of bee, efficiently can reach 98%.
Can be found out by following table, the present invention adopts this kind of raw material proportioning, compares with sodium bicarbonate with simple use salt, and it is controlledThe effect for the treatment of increases significantly, and the infection of bee larva is completely controlled, does not have new infection to occur, there is no dead larvaContinue to be hauled out honeycomb, open beehive, in beehive, larva is in good condition,
The present invention compares with the 5th group with the 6th group, and the present invention has not only simplified the process of medicine, and the present inventionMedication effect is good, easy to carry, and medicine of the present invention is powder, easy to carry, and easy to process, the 6th group and the 7thGroup is to adopt Chinese medicine to decoct, and generally puts honeybee in remoter position owing to putting honeybee people, carries a large amount of raw materials, combustionMaterial and water inconvenience, this just gives in time and treats and brought inconvenience, and the present invention adopts said ratio not only result for the treatment of is good, andBe convenient for carrying with simple and easy to use.
The present invention is compared with the 7th group, although the 7th group carry is also more convenient, result for the treatment of is undesirable.
By theory of traditional Chinese medical science, chalk disease is that sour poison causes, medicine of the present invention is combined with special efficacy, and in general three days, honeybee stopsInfect, after medication, no longer recurrence, prevents water breakthrough in use, and water breakthrough effect is very bad.
Table one
Claims (2)
1. a medicine for the treatment of ascospherosis of bee, is characterized in that: the bulk drug by following weight portion is made: yellow 5 parts of dishes of palpus,40 parts of 5 parts of the capsules of weeping forsythia, 5 parts of inular flowers, 5 parts of clam skins, 40 parts of dietary alkalis and salt;
The preparation method of the medicine of this treatment ascospherosis of bee, comprises the following steps:
1), get the raw materials ready according to above-mentioned raw materials:
2), by Huang must dish airing 10-15 days, until dry completely, be then ground into smalls, obtain smalls A;
3), by the capsule of weeping forsythia clean dried and crushed become smalls, obtain smalls B;
4), by inular flower clean dried and crushed become smalls, obtain smalls C;
5), clam skin is ground into smalls, obtain smalls D;
6), above-mentioned smalls A, smalls B, smalls C, smalls D, dietary alkali and salt are fully mixed and both obtain medicine.
2. a kind of medicine for the treatment of ascospherosis of bee as claimed in claim 1, is characterized in that: clam skin high temperature in step 5)Be heated to lime shape, then pulverize.
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| CN1113758A (en) * | 1994-05-21 | 1995-12-27 | 高承沂 | Preparing method for Baiemie (a preparation for treating chalk brood) |
| CN1142948A (en) * | 1995-08-11 | 1997-02-19 | 谢理清 | New medicine-keeding for curing chalk brood of honeybee larva |
| CN103055038A (en) * | 2012-12-05 | 2013-04-24 | 青岛绿曼生物工程有限公司 | Pure Chinese medicinal composition for treating bee chalkbrood disease and preparation method thereof |
| CN103055031A (en) * | 2012-12-05 | 2013-04-24 | 青岛绿曼生物工程有限公司 | Pure Chinese medicinal composition for treating lime disease of bees and preparation method of pure Chinese medicinal composition |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101311192B1 (en) * | 2011-07-05 | 2013-09-26 | 바이오텐주식회사 | Production method of the Lactobacilus spp. using the medium, and method of cure of herny bee's diseases |
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2014
- 2014-01-24 CN CN201410034380.0A patent/CN103735628B/en active Active
Patent Citations (4)
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|---|---|---|---|---|
| CN1113758A (en) * | 1994-05-21 | 1995-12-27 | 高承沂 | Preparing method for Baiemie (a preparation for treating chalk brood) |
| CN1142948A (en) * | 1995-08-11 | 1997-02-19 | 谢理清 | New medicine-keeding for curing chalk brood of honeybee larva |
| CN103055038A (en) * | 2012-12-05 | 2013-04-24 | 青岛绿曼生物工程有限公司 | Pure Chinese medicinal composition for treating bee chalkbrood disease and preparation method thereof |
| CN103055031A (en) * | 2012-12-05 | 2013-04-24 | 青岛绿曼生物工程有限公司 | Pure Chinese medicinal composition for treating lime disease of bees and preparation method of pure Chinese medicinal composition |
Non-Patent Citations (2)
| Title |
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| 富养蜂与白垩病防治;张建忠，等;《中国蜂业》;20130630;第64卷;第27页 * |
| 纯碱治白垩病有效;翁受椿;《中国养蜂》;19971231(第6期);第19页 * |
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