CN103055038B - Pure Chinese medicinal composition for treating bee chalkbrood disease and preparation method thereof - Google Patents
Pure Chinese medicinal composition for treating bee chalkbrood disease and preparation method thereof Download PDFInfo
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Abstract
The invention relates to a pure Chinese medicinal composition for treating bee chalkbrood disease. The pure Chinese medicinal composition is prepared by mixing and extracting the following components in part by weight: 25 to 35 parts of dried alum, 25 to 45 parts of borneol, 15 to 35 parts of safflower, 15 to 35 parts of coptis root, 15 to 35 parts of snake gall, 15 to 35 parts of cortex pseudolaricis, 15 to 35 parts of liquoric root, 15 to 35 parts of wrinkled gianthyssop herb, 25 to 35 parts of blackberry lily and 25 to 35 parts of Chinese wolfberry root-bark. The pure Chinese medicinal composition has the advantages that the composition is reasonable in formula and easy to prepare, does not have any toxic or side effects, treats both symptoms and root causes, and has a very obvious effect; medicine residues and medicine resistance are avoided; and the clinical results prove that the pure Chinese medicinal composition has a special effect of treating bee chalkbrood disease.
Description
Technical field
The present invention relates to pure Chinese medicine composition and preparation method thereof for the treatment of ascospherosis of bee, belong to field of animal, be particularly a kind ofly specifically designed to pure Chinese medicine composition for the treatment of ascospherosis of bee and preparation method thereof.
Background technology
Ascospherosis of bee has another name called calcium carbonate disease, is to cause a kind of fungal infectious disease of bee larvae death by Ascosphaera apis parasitism, is multiplely born in the spring or early summer, particularly easily occurs under overcast and rainy moist environmental condition.Ascosphaera apis is a kind of fungus, only attacks bee larvae.Die of illness larva and contaminated feedstuff, Nidus Vespae etc. is the primary disease source of infection.Propagate mainly through sporangiospore and ascospore.Bee larvae eats the feedstuff of pollution, and spore is sprouted at enteral, grows mycelia can penetrate intestinal wall, and a large amount of mycelia makes enterorrhexis after larva and dead, and forms Sporangium at dead polypide table.After dead larvae, just in pale asphyxia, rear grey or black.Become open-textured Chalk shape thing after larva corpse is withered, body surface is covered with white hypha.
This disease spreads all over more than 100 countries of five continents, is widely distributed.At present, treat this disease the most frequently used be various antifungal drugs, though receive certain effect, because its toxic and side effects is strong, easily produce the problem such as drug resistance and drug residue, clinical practice is very limited or prohibits the use.
Therefore researching and developing a kind of specific medicament for the treatment of ascospherosis of bee is one of this field scientific research technical staff new problem anxious to be resolved.
Summary of the invention
The object of the invention is to overcome above-mentioned weak point, a kind of reasonable recipe is provided, prepare simple, successful, the pure Chinese medicine composition of effectively treating ascospherosis of bee and preparation method thereof.
Implementation of the present invention is as follows for achieving the above object: a kind of pure Chinese medicine composition for the treatment of ascospherosis of bee, and by following component, mixed extraction is formulated by weight: dried Alumen 25-35 part, Borneolum Syntheticum 25-45 part, Flos Carthami 15-35 part, Rhizoma Coptidis 15-35 part, the sub-15-35 part of Fel Serpentis, Cortex Pseudolaricis 15-35 part, Radix Glycyrrhizae 15-35 part, Herba Pogostemonis 15-35 part, Rhizoma Belamcandae 25-35 part, Cortex Lycii 25-35 part.
Described various component optimum ratio amount is: dried Alumen 30 parts, Borneolum Syntheticum 35 parts, 25 parts, Flos Carthami, Rhizoma Coptidis 25 parts, Fel Serpentis 25 parts, Cortex Pseudolaricis 25 parts, 25 parts, Radix Glycyrrhizae, Herba Pogostemonis 25 parts, Rhizoma Belamcandae 30 parts, Cortex Lycii 30 parts.
Treat a preparation method for the pure Chinese medicine composition of ascospherosis of bee, carry out as follows:
(1) 50 mesh sieves are crossed after being pulverized respectively by the various compositions (except dried Alumen, Borneolum Syntheticum) in prescription;
(2) take Flos Carthami 15-35 part, Rhizoma Coptidis 15-35 part, the sub-15-35 part of Fel Serpentis, Cortex Pseudolaricis 15-35 part, Radix Glycyrrhizae 15-35 part, Herba Pogostemonis 15-35 part, Rhizoma Belamcandae 25-35 part, Cortex Lycii 25-35 part by weight, mix 5-10 minute to even;
(3) the 8 times amount water temperatures adding medical material total amount described in (2) are by weight soaked 1 hour (60 DEG C ~ 80 DEG C), then decoct 60 minutes, filter; Gained filtering residue adds 4 times of soak by water 30 minutes again, and filter, merging filtrate, adds dried Alumen 25-35 part, Borneolum Syntheticum 25-45 part, be evaporated to relative density 1.1, embedding, sterilizing, obtain final product.
The medicinal property of each component is as follows:
Dried Alumen: cold, sour, puckery, returns lung, spleen, large intestine, Liver Channel, has the function of heat clearing away expectorant, hemostasis antidiarrheal, dampness removing to stop itchin, removing toxic substances parasite killing; Cure mainly incessant chronic diarrhea, have blood in stool, let out dysentery after heavily etc.
Borneolum Syntheticum: pungent, bitter, cold, GUIXIN, spleen, lung meridian, have the function of sensible refreshment, heat-clearing and fire-dispersing, reducing swelling and alleviating pain; Cure mainly coma convulsions, laryngopharynx swelling and pain, heat in the heart tongue boil, cataract, sore swollen toxin etc.
Flos Carthami: pungent, warm, returns liver, heart channel, have invigorate blood circulation, dissipating blood stasis, pain relieving function; Cure mainly congestion pain, placenta retention, lochiorrhea etc.
Rhizoma Coptidis: bitter, cold, returns liver, the heart, spleen, stomach, gallbladder, large intestine channel, has the function of heat clearing and damp drying, eliminating fire and detoxication; Cure mainly damp-heat dysentery, hyperactivity of heart-fire, excessiveness of stomach-fire, dampness-heat in the liver and gallbladder, conjunctival congestion and swelling pain, fire-toxin carbuncle sore etc.
Fructus Cnidii: acrid, bitter, warm, returns kidney channel, has warming the kidney to invigorate YANG, dampness is dispeled the wind, the function of killing parasites for relieving itching; Cure mainly impotence due to deficiency of the kidney, eczema, pruritus etc.
Cortex Pseudolaricis: pungent, warm, returns lung meridian, has the function of killing parasites for relieving itching; Cure mainly eczema, pruritus, tinea etc.
Radix Glycyrrhizae: sweet, flat, returns lung, the heart, spleen channel, has invigorating the spleen and replenishing QI, expelling phlegm for arresting cough and middle emergency, removing toxic substances, coordinating the actions of various ingredients in a prescription, cushion toxicity, strong function; Cure mainly weakness of the spleen and stomach, cough with asthma, poisoning etc.
Herba Pogostemonis: pungent, tepor, returns lung, spleen, stomach warp, has the function delivering expelling summer-heat, removing dampness by means of aromatics and middle preventing or arresting vomiting; Cure mainly that summer Sunstroke is wet, diarrhea due to summer heat and dampness, regurgitation vomiting, tripe distention and fullness in the abdomen etc.
Rhizoma Belamcandae: bitter, cold, returns lung meridian, has the function of heat-clearing and toxic substances removing, expectorant, sore-throat relieving; Cure mainly cough due to lung-heat, laryngopharynx swelling and pain, accumulation and obstruction of sputum etc.
Cortex Lycii: sweet, cold, returns liver, lung, kidney channel, has that removing heat from blood is brought down a fever, the function of lung heat clearing pathogenic fire reducing; Cure mainly YinXuXueRe, lung-heat cough with asthma etc.
All taste Chinese medicine is according to the theoretical compatible combination of Chinese veterinarian above, for pathogenic microorganism and the clinical symptoms of ascospherosis of bee, plays the effect for the treatment of both the principal and secondary aspects of a disease.
The invention has the beneficial effects as follows: reasonable recipe, preparation is simple, and cost is low, draws materials wide, drug residue free, has no drug resistance, has no side effect, and Chinese medicine extraction is sprayed, curative effect highly significant.The present invention, according to Chinese veterinarian's traditional theory, optimizes this Ten Herbs, and makes them form pure Chinese medicine according to optimum ratio, and clinical practice proves, has specially good effect to treatment ascospherosis of bee.
Detailed description of the invention
Below in conjunction with preferred embodiment, to according to detailed description of the invention provided by the invention, details are as follows:
Embodiment 1
A kind of pure Chinese medicine composition for the treatment of ascospherosis of bee and preparation method thereof: carry out as follows: (every part by 10g)
(1) 50 mesh sieves are crossed after being pulverized respectively by the various compositions (except dried Alumen, Borneolum Syntheticum) in prescription;
(2) take 150 parts, Flos Carthami, Rhizoma Coptidis 150 parts, Fel Serpentis 150 parts, Cortex Pseudolaricis 150 parts, 150 parts, Radix Glycyrrhizae, Herba Pogostemonis 150 parts, Rhizoma Belamcandae 250 parts, Cortex Lycii 250 parts by weight, mix 5-10 minute to even;
(3) the 8 times amount water temperatures adding medical material total amount described in (2) are by weight soaked 1 hour (60 DEG C ~ 80 DEG C), then decoct 60 minutes, filter; Gained filtering residue adds 4 times of soak by water 30 minutes again, and filter, merging filtrate, adds dried Alumen 250 parts, Borneolum Syntheticum 250 parts, be evaporated to relative density 1.1, embedding, sterilizing, obtain final product.
Embodiment 2
A kind of pure Chinese medicine composition for the treatment of ascospherosis of bee and preparation method thereof: carry out as follows: (every part by 10g)
(1) 50 mesh sieves are crossed after being pulverized respectively by the various compositions (except dried Alumen, Borneolum Syntheticum) in prescription;
(2) take 250 parts, Flos Carthami, Rhizoma Coptidis 250 parts, Fel Serpentis 250 parts, Cortex Pseudolaricis 250 parts, 250 parts, Radix Glycyrrhizae, Herba Pogostemonis 250 parts, Rhizoma Belamcandae 300 parts, Cortex Lycii 300 parts by weight, mix 5-10 minute to even;
(3) the 8 times amount water temperatures adding medical material total amount described in (2) are by weight soaked 1 hour (60 DEG C ~ 80 DEG C), then decoct 60 minutes, filter; Gained filtering residue adds 4 times of soak by water 30 minutes again, and filter, merging filtrate, adds dried Alumen 300 parts, Borneolum Syntheticum 350 parts, be evaporated to relative density 1.1, embedding, sterilizing, obtain final product.
Embodiment 3
A kind of pure Chinese medicine composition for the treatment of ascospherosis of bee and preparation method thereof: carry out as follows: (every part by 10g)
(1) 50 mesh sieves are crossed after being pulverized respectively by the various compositions (except dried Alumen, Borneolum Syntheticum) in prescription;
(2) take 350 parts, Flos Carthami, Rhizoma Coptidis 350 parts, Fel Serpentis 350 parts, Cortex Pseudolaricis 350 parts, 350 parts, Radix Glycyrrhizae, Herba Pogostemonis 350 parts, Rhizoma Belamcandae 350 parts, Cortex Lycii 350 parts by weight, mix 5-10 minute to even;
(3) the 8 times amount water temperatures adding medical material total amount described in (2) are by weight soaked 1 hour (60 DEG C ~ 80 DEG C), then decoct 60 minutes, filter; Gained filtering residue adds 4 times of soak by water 30 minutes again, and filter, merging filtrate, adds dried Alumen 350 parts, Borneolum Syntheticum 450 parts, be evaporated to relative density 1.1, embedding, sterilizing, obtain final product.
Clinical trial:
Clinical experiment animal selects Laboratory Diagnosed to be the ill Apis of ascospherosis of bee.Ill honeybee isolation raised separately, be divided into 3 groups at random, often organize 3 casees totally 15 spleen honeybees, 3 groups of feeding environments are identical, and experimental period carries out 5 days altogether; Blank group is left intact, and drug control group institute's control drug uses treatment by its description; Product of the present invention (compositions obtained by the embodiment of the present invention 1) is mixed homogeneously in 3: 2 ratios with distilled water by present composition group, sprays spleen every day 1 time, continuous 4 days.Computational methods: mortality rate=dead animal number/experimental animal number × 100%, cure rate=healing number of animals/experimental animal number × 100%.Following table is the result (testing the 4th day) at the end of experiment.
Table is that the applicable cases that the present invention and other medicines treat ascospherosis of bee contrasts:
| Numbering | Group | Mortality rate % | Cure rate % | At the end of have symptom rate % |
| I | Blank group | 21 | 54 | 25 |
| II | Griseofulvin spray group | 10 | 80 | 10 |
| III | Present composition group | 6 | 87 | 7 |
This clinical effectiveness statistical analysis, present composition treatment group and griseofulvin spray group in cure rate compared with significant difference (P < 0.05).
Safety testing:
Choose healthy Apis 1 case 5 spleen, 5 times according to the made compositions recommended dose of the embodiment of the present invention 1 are sprayed 4 times continuously, during medication, healthy Apis is without any bad or Deviant Behavior performance, and difference is not remarkable compared with blank group (i.e. health not administration group).
Above-mentioned detailed description of pure Chinese medicine composition of this treatment ascospherosis of bee and preparation method thereof being carried out with reference to embodiment; illustrative instead of determinate; several embodiments can be listed according to institute's limited range; therefore in the change do not departed under general plotting of the present invention and amendment, should belong within protection scope of the present invention.
Claims (1)
1. treat a preparation method for the pure Chinese medicine composition of ascospherosis of bee in the mode of spraying, it is characterized in that:
Component is:
Dried Alumen 25-35 part, Borneolum Syntheticum 25-45 part, Flos Carthami 15-35 part, Rhizoma Coptidis 15-35 part, the sub-15-35 part of Fel Serpentis, Cortex Pseudolaricis 15-35 part, Radix Glycyrrhizae 15-35 part, Herba Pogostemonis 15-35 part, Rhizoma Belamcandae 25-35 part, Cortex Lycii 25-35 part;
Method is:
(1) the various compositions except dried Alumen, Borneolum Syntheticum in prescription are pulverized rear mistake 50 mesh sieve respectively;
(2) take Flos Carthami 15-35 part, Rhizoma Coptidis 15-35 part, the sub-15-35 part of Fel Serpentis, Cortex Pseudolaricis 15-35 part, Radix Glycyrrhizae 15-35 part, Herba Pogostemonis 15-35 part, Rhizoma Belamcandae 25-35 part, Cortex Lycii 25-35 part by weight, mix 5-10 minute to even;
(3) the 8 times amount water temperatures adding medical material total amount described in (2) by weight soak 1 hour, and temperature is 60 DEG C ~ 80 DEG C, then decoct 60 minutes, filter; Gained filtering residue adds 4 times of soak by water 30 minutes again, and filter, merging filtrate, adds dried Alumen 25-35 part, Borneolum Syntheticum 25-45 part, be evaporated to relative density 1.1, embedding, sterilizing, obtain final product.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201210549159.XA CN103055038B (en) | 2012-12-05 | 2012-12-05 | Pure Chinese medicinal composition for treating bee chalkbrood disease and preparation method thereof |
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| CN201210549159.XA CN103055038B (en) | 2012-12-05 | 2012-12-05 | Pure Chinese medicinal composition for treating bee chalkbrood disease and preparation method thereof |
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| CN103055038A CN103055038A (en) | 2013-04-24 |
| CN103055038B true CN103055038B (en) | 2014-12-31 |
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| CN201210549159.XA Expired - Fee Related CN103055038B (en) | 2012-12-05 | 2012-12-05 | Pure Chinese medicinal composition for treating bee chalkbrood disease and preparation method thereof |
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| CN103735628B (en) * | 2014-01-24 | 2016-05-11 | 刘兴华 | A kind of medicine for the treatment of ascospherosis of bee and preparation method thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4504495A (en) * | 1984-02-24 | 1985-03-12 | Keltner Lawrence C | Process for the control of chalkbrood disease in leafcutter bees |
| CN1251713C (en) * | 2003-11-07 | 2006-04-19 | 王希颜 | Chinese traditional medicine preparation for preventing and treating bee diseases |
| CN102273495A (en) * | 2010-06-11 | 2011-12-14 | 滕纯森 | Baofengjian |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20000007406A (en) * | 1998-07-03 | 2000-02-07 | 서종복 | Control drossing for chalkbrood disease of honey bee and a process for controlling chalkbrood disease of honey bee |
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- 2012-12-05 CN CN201210549159.XA patent/CN103055038B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4504495A (en) * | 1984-02-24 | 1985-03-12 | Keltner Lawrence C | Process for the control of chalkbrood disease in leafcutter bees |
| CN1251713C (en) * | 2003-11-07 | 2006-04-19 | 王希颜 | Chinese traditional medicine preparation for preventing and treating bee diseases |
| CN102273495A (en) * | 2010-06-11 | 2011-12-14 | 滕纯森 | Baofengjian |
Non-Patent Citations (3)
| Title |
|---|
| 中草药在养蜂业中的应用现状及展望;李旭涛等;《中国养蜂》;20031231;第54卷(第01期);第32页右栏第1段第1段 * |
| 如何防治蜜蜂白垩病;石钢;《中国蜂业》;20071231;第58卷(第10期);第22页右栏第6段 * |
| 白垩病研究进展;赵磊等;《中兽医医药杂志》;20061231(第02期);第63页右栏第11行 * |
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