CN102823426B - Simulated wild ganoderma atrum tree section culturing technology - Google Patents
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Abstract
The invention discloses a simulated wild ganoderma atrum tree section culturing technology. The simulated wild ganoderma atrum tree section culturing technology includes firstly preparing a culturing material bag; selecting a hard broad-leaved tree, sawing the hard broad-leaved tree into short sections, packaging the short sections and disposing the same into a steam pot to steam; secondly, sterilizing: sterilizing a seeding chamber with blended agentia ; thirdly, seeding: cooling the culturing material bag and grafting hyphae thereon; fourthly, culturing the hyphae; fifthly, digging a rest-balk: digging the rest-balk on loose soil, and digging a ganoderma atrum bed in the rest-balk; sixthly, covering earth; seventhly, managing; and eighthly, harvesting. The simulated wild ganoderma atrum tree section culturing technology is simple and easy and low in economical cost, simulated wild ganoderma atrum cultured is high in effective components and fine in economic benefits and has wide development prospect.
Description
Technical field
The present invention relates to a kind of cultivation method of imitative wild Black Ganoderma, relate in particular to the culture technique in the ripe wooden cross section of a kind of imitative wild Black Ganoderma.
Background technology
Black Ganoderma is annual fungi, has stem, and suberin is one of numerous precious Chinese herbal medicines of chinese tradition medical science.It has dual regulation to human body, and the kind of curing the disease relates to each systems such as cardiovascular and cerebrovascular, digestion, nerve, internal secretion, breathing, motion.The effect wherein also with treatment various diseases.Antitumor: Black Ganoderma can significantly improve the immunologic function of body, strengthen patient's self resistance against diseases, inhibition cancer cell increases, and is the preferred agents of assistant treating cancer.Protecting liver and detoxication: can promote the metabolism of liver to medicine, poisonous substance, effectively improve liver function, protection liver.To treating various chronic hepatitiss, chronic poisoning has definite curative effect.Anti-ageing: its polysaccharide containing, polypeptide have promotion and adjust immunologic function; Regulate metabolic balance; Promote the synthetic of nucleic acid and protein; Significantly promote that cell nuclear dna is synthetic; Obvious delaying cell aging, the beauty treatment youth, promotes longevity.Angiocardiopathy preventing: effectively coronary artery dilator, increase CF, improve Myocardial Microcirculation, strengthen the supply of myocardium oxygen and energy, significantly reduce cholesterol, lipoprotein and triglyceride.Neurasthenia: glossy ganoderma has stronger adjusting, inhibitory action to central nervous system, has the effect of tranquilizing and allaying excitement.Neurasthenia, insomniac are had to significant curative effect.Treatment hypertension: can make Blood pressure drop and there is the effect that extends and stablize other antihypertensive drugs.Treatment diabetes: the water-soluble polysaccharide in glossy ganoderma can alleviate the occurring degree of Non-Insulin Dependent Diabetes Mellitus.Treatment chronic bronchitis: have significantly the effect of calming, eliminate the phlegm, relieving asthma.Beauty treatment: can regulation of skin moisture, recover skin elasticity, make skin fine and smooth, moistening, and can suppress melanic formation and the precipitation in skin, there is the effects such as beauty care, beauty treatment.Can improve skin microcirculation, eliminate free radical, eliminate the functions such as skin surface brown pigment deposition, thereby play the effect of moisturizing, beauty treatment.Regulate sleep, to menstrual disorder, through closing the gynecological disease of dysmenorrhoea, have good effect.
At present, the cultivation method of wild Black Ganoderma mainly adopts booth to cultivate, and usings wood chip and wheat bran as medium, and the Black Ganoderma of producing has certain value, but compares with wild Black Ganoderma, and drug value has very large difference.The culture technique in the ripe wooden cross section of a kind of imitative wild Black Ganoderma provided by the invention, simple to operate, convenient and easy, the Black Ganoderma cultivating has the following advantages: 1) glossy ganoderma quality better: ripe segment wood cultivated glossy ganoderma great advantage is that ganoderma lucidum fruitbody cap is abundant, roomy, color and luster is vivid, containing polysaccharide body and organic germanium, enrich; Content of beary metal is extremely low, can not use any agriculture pharmaceutical compositions, pure natural growth; 2) biological transformation ratio is higher: Duan Mu is planted in the every kind of several years of once can growing continuously in the situation of control termites, varied bacteria growing, and large Duan Mu even can gather in the crops every year two batches for eight, nine years, until section wood has rotted; 3) good in economic efficiency: the Black Ganoderma nutrition content of imitating wild planting compares the content close to pure Wild ganoderma, and pollution-free; 4) yield rate is high, qualification rate is high: owing to making the nutrition of Duan Muzhong effectively be degraded by high temperature, high pressure, easily by Ganoderma lucidum mycelium, decomposed and absorb, adding high-temperature sterilization, pollution rate is low.
Summary of the invention
One object of the present invention is to provide a kind of culture technique of imitative wild Black Ganoderma, and another object is to provide the culture technique in the ripe wooden cross section of a kind of imitative wild Black Ganoderma.
For this reason, the invention provides the culture technique in the ripe wooden cross section of a kind of imitative wild Black Ganoderma, comprise the following steps:
1) cultivate the preparation of construction materials contract: select wooden harder, the broad-leaved trees such as the maple tree of diameter between 15～18cm, chestnut tree, be sawn into short section of wood of 20～30cm left and right, with ethene bacterium bag, package and be placed in steamer, high temperature burns after 100 ℃, continues boiling 12～15h, and high temperature high cooking object is in order to make short section of wooden boiling well-done, there is good plantation condition, be convenient to above mycelia is seeded in better;
2) sterilizing: transfer room is carried out to sterilization treatment, first 15g potassium permanganate and 20ml formaldehyde are dissolved in the clear water of 500ml, stir, using it as thimerosal, transfer room is carried out to spraying disinfection and process 25min; Secondly the ammoniacal liquor of 12ml is added in the middle of the clear water of 500ml, transfer room is carried out to all-out atomizing sterilization treatment 30min; Again 10ml Peracetic acid is added in the middle of 500ml clear water, transfer room is carried out to all-out atomizing sterilization treatment 30min;
3) inoculation: cultivate construction materials contract and carry out normal temperature sterilizing 36h having processed 12～15h at 100 ℃ of high temperature, while being cooled to 28～30 ℃ of left and right, move into aseptic inoculation chamber, open with the packaged cultivation construction materials contract of ethene bacterium bag, mycelium grafting is got on, in the time of by grafting, guarantee cleaning-less bacteria infection, avoid miscellaneous bacteria to breed growth;
4) cultural hypha: it is 25～30 ℃ that the ripe wooden cross section of having inoculated is placed in to temperature, and humidity is 65%～75%, cultivates 20～25d under the environment of dark or the low light level, until mycelia is covered with bag;
5) open furrow: on the loose sandy soil that is 6～7 at PH, be long 15～20m, wide 2m, the furrow of high 20～30cm, surrounding is established drain ditch, digs the sesame bed that 18～25cm is dark in furrow;
6) earthing: when sesame bed tempertaure is 25～30 ℃, cultivation bag is opened, connect cultivation construction materials contract and cut supine ripe raft in bed, spacing is between 0.5～1m, wraps the earthing that 3～4cm is dark, and keeping soil moisture is 70%～75%;
7) management: reach 90% in humidity, temperature is in 25～32 degree left and right, on ripe wooden cross section, can grow the young sesame of several picture fawn angular shapes, now young sesame is dredged to sesame work, a little less than going, stay by force, a young sesame is stayed in each ripe wooden cross section, when stem grows to 6～7cm, allow its ventilation and penetrating light, keep humidity to be not less than 90%;
8) gather: ganoderma lucidum fruitbody was unearthed after 2～March, can loosen the soil glossy ganoderma is not destroyed to mycelia harvesting.
Preferably, in the described ripe wooden cross section of a kind of imitative wild Black Ganoderma culture technique, described cultivation construction materials contract also can be short section of wood 70%～85%, weed tree sawdust 10%～13%, wheat bran 10%～12% tapioca starch 5%～7%, cream powder 0.8%～1%, white sugar 0.5%～1%, potassium chloride, magnesium sulfate, nitrogenous fertilizer trace.
Preferably, in the described ripe wooden cross section of a kind of imitative wild Black Ganoderma culture technique, the specification of described ethene bacterium bag is: long 62～65cm, wide 35～40cm, thick 3mm, generally can fill composts or fertilisers of cultivating 6～10kg.
Preferably, in the described ripe wooden cross section of a kind of imitative wild Black Ganoderma culture technique, described transfer room is the shed that wood is built, high 1.8～2.0m, ceiling weeds sunshade, weak printing opacity, good with diaphragm seal in canopy, the circulation of isolated shed and outside air before inoculation.
Preferably, in the described ripe wooden cross section of a kind of imitative wild Black Ganoderma culture technique, in described seeded process, hand and instrument will just can carry out inoculation work with after the wiping of the appropriate potassium permanganate aqueous solution, after having inoculated, transfer room door is opened allowing air flow 30min.
The ripe wooden cross section of a kind of imitative wild Black Ganoderma of the present invention culture technique, simple to operate, convenient and easy, change indoor greenhouse cultivation pattern, greatly reduced the production cost of enterprise.
And the imitative wild Black Ganoderma active drug composition cultivating is high, has very high economic worth.
Accompanying drawing explanation
Fig. 1 is the ripe wooden cross section of a kind of imitative wild Black Ganoderma of the present invention culture technique.
Embodiment
Below in conjunction with accompanying drawing, the present invention is described in further detail, to make those skilled in the art can implement according to this with reference to specification word.
Embodiment 1, the ripe wooden cross section of a kind of imitative wild Black Ganoderma culture technique, comprise the steps:
1) cultivate the preparation of construction materials contract: select the broad-leaved trees such as wooden harder, the maple tree of diameter between 15cm, chestnut tree, be sawn into short section of wood of 20cm left and right, package be placed in steamer with ethene bacterium bag, high temperature burns after 100 ℃, lasting boiling 12h;
2) sterilizing: transfer room is carried out to sterilization treatment, first 15g potassium permanganate and 20ml formaldehyde are dissolved in the clear water of 500ml, stir, using it as thimerosal, transfer room is carried out to spraying disinfection and process 25min; Secondly the ammoniacal liquor of 12ml is added in the middle of the clear water of 500ml, transfer room is carried out to all-out atomizing sterilization treatment 30min; Again 10ml Peracetic acid is added in the middle of 500ml clear water, transfer room is carried out to all-out atomizing sterilization treatment 30min;
3) inoculation: cultivate construction materials contract and carry out normal temperature sterilizing 36h having processed 12h at 100 ℃ of high temperature, while being cooled to 28 ℃ of left and right, move into aseptic inoculation chamber, open with the packaged cultivation construction materials contract of ethene bacterium bag, mycelium grafting is got on, in the time of by grafting, guarantee cleaning-less bacteria infection, avoid miscellaneous bacteria to breed growth;
4) cultural hypha: it is 28 ℃ that the ripe wooden cross section of having inoculated is placed in to temperature, and humidity is 65%, cultivates 20d under the environment of dark or the low light level, until mycelia is covered with bag;
5) open furrow: on the loose sandy soil that is 6 at PH, be long 15m, wide 2m, the furrow of high 20cm, surrounding is established drain ditch, digs the sesame bed that 18cm is dark in furrow;
6) earthing: when sesame bed tempertaure is 25 ℃, cultivation bag is opened, connect cultivation construction materials contract and cut supine ripe raft in bed, spacing is between 0.5m, wraps the earthing that 3cm is dark, and keeping soil moisture is 70%;
7) management: reach 90% in humidity, temperature is in 25 ℃ of left and right, on ripe wooden cross section, can grow the young sesame of several picture fawn angular shapes, now young sesame is dredged to sesame work, a little less than going, stay by force, a young sesame is stayed in each ripe wooden cross section, when stem grows to 6cm, allow its ventilation and penetrating light, keep humidity to be not less than 90%;
8) gather: ganoderma lucidum fruitbody was unearthed after March, can loosen the soil glossy ganoderma is not destroyed to mycelia harvesting.
Embodiment 2, the ripe wooden cross section of a kind of imitative wild Black Ganoderma culture technique, comprise the steps:
1) cultivate the preparation of construction materials contract: select the broad-leaved trees such as wooden harder, the maple tree of diameter between 16cm, chestnut tree, be sawn into short section of wood of 25cm left and right, package be placed in steamer with ethene bacterium bag, high temperature burns after 100 ℃, lasting boiling 13h;
2) sterilizing: transfer room is carried out to sterilization treatment, first 15g potassium permanganate and 20ml formaldehyde are dissolved in the clear water of 500ml, stir, using it as thimerosal, transfer room is carried out to spraying disinfection and process 25min; Secondly the ammoniacal liquor of 12ml is added in the middle of the clear water of 500ml, transfer room is carried out to all-out atomizing sterilization treatment 30min; Again 10ml Peracetic acid is added in the middle of 500ml clear water, transfer room is carried out to all-out atomizing sterilization treatment 30min;
3) inoculation: cultivate construction materials contract and carry out normal temperature sterilizing 36h having processed 13h at 100 ℃ of high temperature, while being cooled to 26 ℃ of left and right, move into aseptic inoculation chamber, open with the packaged cultivation construction materials contract of ethene bacterium bag, mycelium grafting is got on, in the time of by grafting, guarantee cleaning-less bacteria infection, avoid miscellaneous bacteria to breed growth;
4) cultural hypha: it is 26 ℃ that the ripe wooden cross section of having inoculated is placed in to temperature, and humidity is 69%, cultivates 22d under the environment of dark or the low light level, until mycelia is covered with bag;
5) open furrow: on the loose sandy soil that is 7 at PH, be long 16m, wide 2m, the furrow of high 222cm, surrounding is established drain ditch, digs the sesame bed that 20cm is dark in furrow;
6) earthing: when sesame bed tempertaure is 29 ℃, cultivation bag is opened, connect cultivation construction materials contract and cut supine ripe raft in bed, spacing is between 0.6m, wraps the earthing that 3cm is dark, and keeping soil moisture is 71;
7) management: reach 90% in humidity, temperature is in 26 ℃ of left and right, on ripe wooden cross section, can grow the young sesame of several picture fawn angular shapes, now young sesame is dredged to sesame work, a little less than going, stay by force, a young sesame is stayed in each ripe wooden cross section, when stem grows to 6cm, allow its ventilation and penetrating light, keep humidity to be not less than 90%;
8) gather: ganoderma lucidum fruitbody was unearthed after March, can loosen the soil glossy ganoderma is not destroyed to mycelia harvesting.
Embodiment 3, the ripe wooden cross section of a kind of imitative wild Black Ganoderma culture technique, comprise the steps:
1) cultivate the preparation of construction materials contract: select the broad-leaved trees such as wooden harder, the maple tree of diameter between 16cm, chestnut tree, be sawn into short section of wood of 30cm left and right, package be placed in steamer with ethene bacterium bag, high temperature burns after 100 ℃, lasting boiling 15h;
2) sterilizing: transfer room is carried out to sterilization treatment, first 15g potassium permanganate and 20ml formaldehyde are dissolved in the clear water of 500ml, stir, using it as thimerosal, transfer room is carried out to spraying disinfection and process 25min; Secondly the ammoniacal liquor of 12ml is added in the middle of the clear water of 500ml, transfer room is carried out to all-out atomizing sterilization treatment 30min; Again 10ml Peracetic acid is added in the middle of 500ml clear water, transfer room is carried out to all-out atomizing sterilization treatment 30min;
3) inoculation: cultivate construction materials contract and carry out normal temperature sterilizing 36h having processed 15h at 100 ℃ of high temperature, while being cooled to 30 ℃ of left and right, move into aseptic inoculation chamber, open with the packaged cultivation construction materials contract of ethene bacterium bag, mycelium grafting is got on, in the time of by grafting, guarantee cleaning-less bacteria infection, avoid miscellaneous bacteria to breed growth;
4) cultural hypha: it is 30 ℃ that the ripe wooden cross section of having inoculated is placed in to temperature, and humidity is 75%, cultivates 25d under the environment of dark or the low light level, until mycelia is covered with bag;
5) open furrow: on the loose sandy soil that is 7 at PH, be long 20m, wide 2m, the furrow of high 25cm, surrounding is established drain ditch, digs the sesame bed that 22cm is dark in furrow;
6) earthing: when sesame bed tempertaure is 30 ℃, cultivation bag is opened, connect cultivation construction materials contract and cut supine ripe raft in bed, spacing is between 1m, wraps the earthing that 4cm is dark, and keeping soil moisture is 72%;
7) management: reach 90% in humidity, temperature is in 30 ℃ of left and right, on ripe wooden cross section, can grow the young sesame of several picture fawn angular shapes, now young sesame is dredged to sesame work, a little less than going, stay by force, a young sesame is stayed in each ripe wooden cross section, when stem grows to 7cm, allow its ventilation and penetrating light, keep humidity to be not less than 90%;
8) gather: ganoderma lucidum fruitbody was unearthed after February, can loosen the soil glossy ganoderma is not destroyed to mycelia harvesting.
Embodiment 4, the ripe wooden cross section of a kind of imitative wild Black Ganoderma culture technique, comprise the steps:
1) cultivate the preparation of construction materials contract: select the broad-leaved trees such as wooden harder, the maple tree of diameter between 17cm, chestnut tree, be sawn into short section of wood of 25cm left and right, package be placed in steamer with ethene bacterium bag, high temperature burns after 100 ℃, lasting boiling 14h;
2) sterilizing: transfer room is carried out to sterilization treatment, first 15g potassium permanganate and 20ml formaldehyde are dissolved in the clear water of 500ml, stir, using it as thimerosal, transfer room is carried out to spraying disinfection and process 25min; Secondly the ammoniacal liquor of 12ml is added in the middle of the clear water of 500ml, transfer room is carried out to all-out atomizing sterilization treatment 30min; Again 10ml Peracetic acid is added in the middle of 500ml clear water, transfer room is carried out to all-out atomizing sterilization treatment 30min;
3) inoculation: cultivate construction materials contract and carry out normal temperature sterilizing 36h having processed 14h at 100 ℃ of high temperature, while being cooled to 29 ℃ of left and right, move into aseptic inoculation chamber, open with the packaged cultivation construction materials contract of ethene bacterium bag, mycelium grafting is got on, in the time of by grafting, guarantee cleaning-less bacteria infection, avoid miscellaneous bacteria to breed growth;
4) cultural hypha: it is 29 ℃ that the ripe wooden cross section of having inoculated is placed in to temperature, and humidity is 70%, cultivates 24d under the environment of dark or the low light level, until mycelia is covered with bag;
5) open furrow: on the loose sandy soil that is 6 at PH, be long 18m, wide 2m, the furrow of high 28cm, surrounding is established drain ditch, digs the sesame bed that 25cm is dark in furrow;
6) earthing: when sesame bed tempertaure is 28 ℃, cultivation bag is opened, connect cultivation construction materials contract and cut supine ripe raft in bed, spacing is between 0.8m, wraps the earthing that 4cm is dark, and keeping soil moisture is 75%;
7) management: reach 90% in humidity, in the time of 31 ℃ of left and right of temperature, on ripe wooden cross section, can grow the young sesame of several picture fawn angular shapes, now young sesame is dredged to sesame work, a little less than going, stay by force, a young sesame is stayed in each ripe wooden cross section, when stem grows to 6cm, allow its ventilation and penetrating light, keep humidity to be not less than 90%;
8) gather: ganoderma lucidum fruitbody was unearthed after March, can loosen the soil glossy ganoderma is not destroyed to mycelia harvesting.
Although embodiment of the present invention are open as above, but it is not restricted to listed utilization in specification and embodiment, it can be applied to various applicable the field of the invention completely, for those skilled in the art, can easily realize other modification, therefore do not deviating under the universal that claim and equivalency range limit, the present invention is not limited to specific details and illustrates here and the legend of describing.
Claims (5)
1. an imitative wild Black Ganoderma trees cross section cultivation method, is characterized in that, comprises the following steps:
1) cultivate the preparation of construction materials contract: select wooden harder, maple tree, the chestnut of diameter between 15～18cm set broad-leaved tree, is sawn into the short section of wood of 20～30cm, with ethene bacterium bag, packages and is placed in steamer, high temperature burns after 100 ℃, continues boiling 12～15h;
2) sterilizing: transfer room is carried out to sterilization treatment, first 15g potassium permanganate and 20ml formaldehyde are dissolved in the clear water of 500ml, stir, using it as thimerosal, transfer room is carried out to spraying disinfection and process 25min; Secondly the ammoniacal liquor of 12ml is added in the middle of the clear water of 500ml, transfer room is carried out to all-out atomizing sterilization treatment 30min; Again 10ml Peracetic acid is added in the middle of 500ml clear water, transfer room is carried out to all-out atomizing sterilization treatment 30min;
3) inoculation: cultivate construction materials contract and carry out normal temperature sterilizing 36h having processed 12～15h at 100 ℃ of high temperature, while being cooled to 28～30 ℃, move into aseptic inoculation chamber, open with the packaged cultivation construction materials contract of ethene bacterium bag, mycelium grafting is got on, in the time of by grafting, guarantee cleaning-less bacteria infection, avoid miscellaneous bacteria to breed growth;
4) cultural hypha: it is 25～30 ℃ that the trees cross section of having inoculated is placed in to temperature, and humidity is 65%～75%, cultivates 20～25d under the environment of dark or the low light level, until mycelia is covered with bag;
5) open furrow: on the loose sandy soil that is 6～7 at PH, be long 15～20m, wide 2m, the furrow of high 20～30cm, surrounding is established drain ditch, digs the sesame bed that 18～25cm is dark in furrow;
6) earthing: when sesame bed tempertaure is 25～30 ℃, cultivation bag is opened, connect and cultivate construction materials contract and cut supine trees and arrange in bed, spacing is between 0.5～1m, wraps the earthing that 3～4cm is dark, and keeping soil moisture is 70%～75%;
7) management: reach 90% in humidity, temperature is in 25～32 ℃, on trees cross section, can grow the young sesame of several picture fawn angular shapes, now young sesame is dredged to sesame work, a little less than going, stay by force, a young sesame is stayed in each trees cross section, when stem grows to 6～7cm, allow its ventilation and penetrating light, keep humidity to be not less than 90%;
8) gather: ganoderma lucidum fruitbody was unearthed after 2～March, loosens the soil glossy ganoderma is not destroyed to mycelia harvesting.
2. a kind of imitative wild Black Ganoderma trees according to claim 1 cross section cultivation method, it is characterized in that described cultivation construction materials contract is short section of wood 70%～85%, weed tree sawdust 10%～13%, wheat bran 10%～12%, tapioca starch 5%～7%, land plaster 0.8%～1%, white sugar 0.5%～1%, potassium chloride, magnesium sulfate, nitrogenous fertilizer trace.
3. a kind of imitative wild Black Ganoderma trees according to claim 1 cross section cultivation method, is characterized in that the specification of described ethene bacterium bag is: long 62～65cm, wide 35～40cm, thick 3mm, can fill composts or fertilisers of cultivating 6～10kg.
4. a kind of imitative wild Black Ganoderma trees according to claim 1 cross section cultivation method, is characterized in that described transfer room is the shed that wood is built, high 1.8～2.0m, ceiling weeds sunshade, weak printing opacity, good with diaphragm seal in canopy, the circulation of isolated shed and outside air before inoculation.
5. a kind of imitative wild Black Ganoderma trees according to claim 1 cross section cultivation method, it is characterized in that in described seeded process, hand and instrument will just can carry out inoculation work with after appropriate potassium permanganate solution wiping, and after having inoculated, transfer room door is opened allowing air flow 30min.
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| CN103238467B (en) * | 2013-05-23 | 2015-03-25 | 袁学军 | Method for artificially cultivating Lucid Ganoderma |
| CN103283485B (en) * | 2013-06-03 | 2015-06-17 | 安徽衡济堂灵芝产业开发有限公司 | Cultivating method for lucid ganoderma |
| CN104145718B (en) * | 2014-08-29 | 2016-04-13 | 刘让云 | A kind of bamboo grove simulated wild glossy ganoderma culture technique |
| CN104206176B (en) * | 2014-09-22 | 2017-03-29 | 江西罗山峰生态科技有限公司 | A kind of wild cultivating method of glossy ganoderma |
| CN105557295A (en) * | 2014-11-05 | 2016-05-11 | 天津市青山玉龙农业科技有限公司 | Mushroom-stick wild cultivation method for lucid ganoderma |
| CN105613037A (en) * | 2015-12-23 | 2016-06-01 | 安徽省霍山县轩芝缘生物科技有限公司 | Wild-simulated cultivation method of ganoderma lucidum karst |
| CN106305139A (en) * | 2016-08-23 | 2017-01-11 | 李军 | Method for growing lucid ganoderma by utilizing fungi grass |
| CN106673722A (en) * | 2016-12-15 | 2017-05-17 | 防城港市蓝瀚达科技有限公司 | Preparation method of eucalyptus wood ganoderma lucidum culture medium |
| CN107018802A (en) * | 2017-05-24 | 2017-08-08 | 贵州黔芝灵绿色农业科技发展有限公司 | A kind of ganoderma lucidum segment wood cultivated method |
| WO2019090453A1 (en) * | 2017-11-07 | 2019-05-16 | 惠州市拓森源生物科技有限公司 | Method for artificially cultivating wild medicinal ganoderma lucidum |
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