CN102795936B - Chinese medicinal herb-seaweed composite biological agent and preparation method thereof - Google Patents

Chinese medicinal herb-seaweed composite biological agent and preparation method thereof Download PDF

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CN102795936B
CN102795936B CN201210324503.5A CN201210324503A CN102795936B CN 102795936 B CN102795936 B CN 102795936B CN 201210324503 A CN201210324503 A CN 201210324503A CN 102795936 B CN102795936 B CN 102795936B
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herbal medicine
marine alga
extracts
biological agent
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CN102795936A (en
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沙志强
张志刚
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Qingdao Fengbang agrochemical Co.,Ltd.
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QINGDAO QINGDAHAIKUI BIOLOGICAL TECHNOLOGY Co Ltd
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Abstract

The invention provides a Chinese medicinal herb-seaweed fertilizer composite biological agent and a preparation method thereof. Effective constituents are extracted from Chinese medicinal herbs and seaweed, which are used as main raw materials, to prepare the biological composite preparation. The Chinese medicinal herb-seaweed fertilizer composite biological agent not only reserves biological active substances in seaweed, but also enhances the drug effect of the Chinese medicinal herbs as insecticide. The biological composite preparation contains rich Chinese medicinal herbs and multiple active substances in seaweed, thereby enhancing the photosynthesis of crops, enhancing the crop yield and product quality, enhancing the drought tolerance, cold tolerance and stress tolerance of the crops, accelerating prematurity and fruit setting rate, preventing and killing various soil insects and enhancing the disease resistance of the crops. The invention has the advantages of low cost, favorable solubility and environmental protection, and is convenient for popularization.

Description

Composite biological agent of a kind of herbal medicine and marine alga and preparation method thereof
Technical field
The present invention relates to biological technical field, specifically refer to a kind of composite biological agent by herbal medicine and seaweed fertilizer and preparation method thereof.
Background technology
At present, herbal medicine has been widely used in the control of agricultural pest as sterilant, solves original chemicals class agricultural chemical insecticide consumption large, residual many, the problem of influence ecological environment and HUMAN HEALTH.
In recent years, marine alga starts to occur as new bio-fertilizer and is applied in agriculture production, because marine alga contains abundant mineral element and various VITAMIN, wherein, the physiologically active substances such as the amino acid contained, mineral substance, polysaccharide, VITAMIN and phytokinin, plant hormone and dormin, effectively promote plant absorbing soil nutrient, moisture and gas; Increase chlorophyll content of plant, effectively improve photosynthetic efficiency; The multiple degeneration-resistant functions such as crop drought resistance, cold-resistant, Resistant can be strengthened, improve crop yield, improve crop quality; Can also Soil structure be improved, improve the multiple efficacies such as retention capacity of soil.
Herbal medicine possesses excellent characteristic and good result of use as sterilant and marine alga separately as bio-feritlizer, if both can be combined, to be ideal agricultural formulations, but, in production process in the past, when herbal medicine and marine alga are carried out composite, due to the complicacy of medicinal herb components, the physiologically active substances such as the amino acid contained in marine alga and polysaccharide are easily destroyed, and are difficult to give full play to the fertilizer efficiency even reducing seaweed fertilizer, therefore, insoluble technical problem is become.
Summary of the invention
For the deficiency that prior art exists, technical problem to be solved by this invention is, composite biological agent of a kind of herbal medicine and seaweed fertilizer and preparation method thereof is provided, for main raw material with natural Chinese medicinal herb and marine alga, through pre-treatment, lixiviate, neutralization, the processing step such as concentrated, composite, extracts active ingredients in herbal medicine and marine alga is made Biological compound preparation, not only remains the physiologically active substance in marine alga, and enhance the property of medicine effect of herbal medicine as sterilant.
For solving the problems of the technologies described above, the technical solution used in the present invention is, composite biological agent of a kind of herbal medicine and marine alga and preparation method thereof, and the composite biological agent of herbal medicine and marine alga comprises following component, and its weight proportion is:
Herbal medicine marine alga mixing base fluid 10 parts-80 parts, humic acids 5 parts-25 parts, 5 parts-25 parts, urea, monoammonium phosphate 5 parts-25 parts, potassium primary phosphate 5 parts-25 parts, potassium sulfate 2 parts-30 parts, trace element 2 parts-20 parts;
Wherein, herbal medicine marine alga mixing base fluid comprises following component, and its weight proportion is:
Extracts of Chinese herbal medicine 20 parts-45 parts, seaweed extracted liquor 50 parts-80 parts, buffer reagent metalloprotease 5 parts-20 parts, 5 parts-20 parts, stablizer wood vinegar.
Above-mentioned herbal medicine and the composite biological agent of marine alga, the main raw material weight proportion of extracts of Chinese herbal medicine is as follows: tuber of stemona 40-70g, bark of eucommia 20-70g, root of langdu 30-80g, Herba Andrographis 30-70g, Herba Sophorae alopecuroidis 10-40g, Radix Astragali 30-80g, root of large-flowered skullcap 20-60g, Herba Houttuyniae 90-130g, kuh-seng 10-40g, Radix Glycyrrhizae 20-50g, garlicin 30-60g, pipe tobacco 80-150g, Rangooncreeper Fruit 20-70g.
Above-mentioned herbal medicine and the composite biological agent of marine alga, the main raw material of seaweed extracted liquor is brown alga.
Above-mentioned herbal medicine and the composite biological agent of marine alga, trace element is one or more in magnesium, copper, manganese, iron, boron, zinc, molybdenum, calcium.
The preparation method of above-mentioned herbal medicine and the composite biological agent of marine alga, comprises following content:
The first step, produces extracts of Chinese herbal medicine: mixing after being cleaned by the herbal raw material of formula ratio, is crushed to 300 orders, extracts by following (1), (3) or (2), (3) two kinds of techniques:
(1) herbal powder is mixed with water, pH value is adjusted to 9-11, add Sumizyme MP and extract 24 hours at 45-65 degree Celsius;
(2) herbal powder is mixed with water, pH value is adjusted to 1-3, add aspartic protease and extract 30 hours at 15-25 degree Celsius;
(3) cool to about 0 degree, pH value is adjusted to 7, be separated and remove residue, then vacuum pressed is concentrated into proportion is that namely 0.8-1.2g/mL obtains extracts of Chinese herbal medicine;
The weight proportion of described herbal raw material each component is: tuber of stemona 40-70g, bark of eucommia 20-70g, root of langdu 30-80g, Herba Andrographis 30-70g, Herba Sophorae alopecuroidis 10-40g, Radix Astragali 30-80g, root of large-flowered skullcap 20-60g, Herba Houttuyniae 90-130g, kuh-seng 10-40g, Radix Glycyrrhizae 20-50g, garlicin 30-60g, pipe tobacco 80-150g, Rangooncreeper Fruit 20-70g;
Second step, produces seaweed extracted liquor: marine alga is cleaned ovendry power and be broken to 300 orders, extracts by (1), (3), (4) or (2), (3), (4) two kinds of techniques:
(1) marine algae powder is mixed with water, pH value is adjusted to 9-11, extract 4-7 hour at 75-100 degree Celsius;
(2) marine algae powder is mixed with water, pH value is adjusted to 1-3, extract 3-5 hour at 45-65 degree Celsius;
(3) pH value is adjusted to 7, then separating and removing impurities;
(4) vacuum pressed is concentrated into proportion is 1-1.4g/mL, namely obtains seaweed extracted liquor;
3rd step, produces herbal medicine marine alga mixing base fluid: fully mixed with seaweed extracted liquor by extracts of Chinese herbal medicine, then add buffer reagent metalloprotease, with stablizer wood vinegar;
The weight proportion of described each component is: extracts of Chinese herbal medicine 20 parts-45 parts, seaweed extracted liquor 50 parts-80 parts, buffer reagent metalloprotease 5 parts-20 parts, 5 parts-20 parts, stablizer wood vinegar;
4th step, makes the composite biological agent of herbal medicine and marine alga: add humic acids, urea, potassium sulfate, monoammonium phosphate, potassium primary phosphate and trace element by herbal medicine marine alga mixing base fluid, mix;
The weight proportion of described each component is: herbal medicine marine alga mixing base fluid 10 parts-80 parts, humic acids 5 parts-25 parts, 5 parts-25 parts, urea, potassium sulfate 2 parts-30 parts, monoammonium phosphate 5 parts-25 parts, potassium primary phosphate 5 parts-25 parts, trace element 2 parts-20 parts.
The preparation method of above-mentioned herbal medicine and the composite biological agent of marine alga, the first step, produces in extracts of Chinese herbal medicine, and the water consumption in (1) or (2) is 10-20 times of herbal powder weight.
The preparation method of above-mentioned herbal medicine and the composite biological agent of marine alga, second step, produces in seaweed extracted liquor, and the water consumption in (1) or (2) is 10-20 times of marine algae powder weight.
The preparation method of above-mentioned herbal medicine and the composite biological agent of marine alga, the first step, produces in extracts of Chinese herbal medicine, and the alkali that adjust ph uses is potassium hydroxide, sodium hydroxide, salt of wormwood or sodium carbonate, and the acid of use is hydrochloric acid, sulfuric acid or phosphoric acid.
The preparation method of above-mentioned herbal medicine and the composite biological agent of marine alga, second step, produces in seaweed extracted liquor, and the alkali that adjust ph uses is potassium hydroxide, sodium hydroxide, salt of wormwood or sodium carbonate, and the acid of use is hydrochloric acid, sulfuric acid or phosphoric acid.
The preparation method of above-mentioned herbal medicine and the composite biological agent of marine alga, trace element is one or more in magnesium, copper, manganese, iron, boron, zinc, molybdenum, calcium.
Tool of the present invention has the following advantages and beneficial effect:
The active substance extraction yield of herbal medicine of the present invention and marine alga is high, do not destroy the effective constituent in herbal medicine, marine alga, containing multiple alkaloid, glycosides sugar, class mushroom sugar, protein, tannin enzyme, auxins benzazolyl compounds, Plant hormones regulators,gibberellins compounds, cytokinin compounds, Tang oligosaccharide, amino acid and magnesium, copper, iron, zinc, boron, molybdenum etc., technique is simple, production cost is lower, and the composite biological agent of herbal medicine and marine alga can be prepared into all kinds of herbal medicine seaweed fertilizer, and effect is better.
Production cost of the present invention is low, and solvability is good, not only has growth promoting effects to plant, the effect of extra-nutrition, and the resistance against diseases that can also improve plant self, repellent and kill subterranean pest-insect, to people and animals and physical environment harmless.Through this organic fertilizer pouring so crop plant not only grows fast, cold-resistant drought resisting.And fruit-setting rate is high, ripe early, painted good, disease resistance ability is strong.
The present invention can be used for the cash crop such as soybean, grape, paddy rice, corn, rape, cotton, strawberry, apple, tobacco, tomato, watermelon, cucumber, sponge gourd, greengrocery, nontoxic, noresidue, not only can coordinate plant growth, improve the ability of Genes For Plant Tolerance disease, and can repellent and kill subterranean pest-insect; Marine alga as fertilizer can improve crop drought resistance, cold-resistant, etc. resistance, multiple highly active nutritive substance can also be provided to crop, promote plant growth, improve product quality, improve crop yield, both combine and not only can improve the utilization ratio of fertilizer, reduce the access times of chemical pesticide, and reduce chemical residuals various in farm crop, alleviate the degree that hardens of soil, improve the breeding of profitable strain in soil, strengthen the soil fertility of soil.
Embodiment
Embodiment 1
The herbal medicine of the present embodiment and the composite biological agent of marine alga, comprise following component, and its weight proportion is:
Herbal medicine marine alga mixing base fluid 10 parts, humic acids 10 parts, 15 parts, urea, monoammonium phosphate 5 parts, potassium primary phosphate 10 parts, potassium sulfate 5 parts, 5 parts, magnesium;
Wherein, herbal medicine marine alga mixing base fluid comprises following component, and its weight proportion is:
Extracts of Chinese herbal medicine 20 parts, seaweed extracted liquor 60 parts, buffer reagent metalloprotease 10 parts, 10 parts, stablizer wood vinegar;
The main raw material weight proportion of extracts of Chinese herbal medicine is as follows: hundred step 40g, bark of eucommia 30g, root of langdu 30g, Herba Andrographis 40g, Herba Sophorae alopecuroidis 20g, Radix Astragali 30g, root of large-flowered skullcap 20g, Herba Houttuyniae 100g, kuh-seng 20g, Radix Glycyrrhizae 20g, garlicin 40g, pipe tobacco 90g Rangooncreeper Fruit 50g;
The main raw material of seaweed extracted liquor is brown alga.
The preparation method of the herbal medicine of the present embodiment and the composite biological agent of marine alga, comprises following content:
The first step, produce extracts of Chinese herbal medicine: mixing after being cleaned by the herbal raw material of formula ratio, is crushed to 300 orders, extracts by following technique:
1, mixed with water by herbal powder, water consumption is 15 times of herbal powder weight, adds 30mol/L potassium hydroxide and pH value is adjusted to 9, adds Sumizyme MP 50 degree of extractions Celsius 24 hours;
2, cool to about 0 degree, add 20mol/L hydrochloric acid and pH value is adjusted to 7, be separated and remove residue, obtain tawny liquid, then vacuum pressed is concentrated into proportion is that namely 0.8g/mL obtains extracts of Chinese herbal medicine;
Wherein, the weight proportion of herbal raw material each component is: tuber of stemona 40g, bark of eucommia 30g, root of langdu 30g, Herba Andrographis 40g, Herba Sophorae alopecuroidis 20g, Radix Astragali 30g, root of large-flowered skullcap 20g, Herba Houttuyniae 100g, kuh-seng 20g, Radix Glycyrrhizae 20g, garlicin 40g, pipe tobacco 90g, Rangooncreeper Fruit 40g.
Second step, produce seaweed extracted liquor: marine alga is cleaned ovendry power and be broken to 300 orders, extract by following technique:
1, mixed with water by marine algae powder, water consumption is 15 times of marine algae powder weight, adds 30mol/L potassium hydroxide and pH value is adjusted to 10, extracts 5 hours at 90 degrees Celsius;
2, add 20mol/L hydrochloric acid and pH value is adjusted to 7, then separating and removing impurities;
3, vacuum pressed is concentrated into proportion is 1.2g/mL, namely obtains seaweed extracted liquor.
3rd step, produces herbal medicine marine alga mixing base fluid: fully mixed with seaweed extracted liquor by extracts of Chinese herbal medicine, then add buffer reagent metalloprotease, stablizer wood vinegar;
The weight proportion of each component is: extracts of Chinese herbal medicine 20 parts, seaweed extracted liquor 60 parts, buffer reagent metalloprotease 10 parts, 10 parts, stablizer wood vinegar.
4th step, makes the composite biological agent of herbal medicine and marine alga: add humic acids, urea, monoammonium phosphate, potassium primary phosphate, potassium sulfate and magnesium by herbal medicine marine alga mixing base fluid, mix;
The weight proportion of each component is: herbal medicine marine alga mixing base fluid 10 parts, humic acids 10 parts, 15 parts, urea, monoammonium phosphate 5 parts, potassium primary phosphate 10 parts, potassium sulfate 5 parts, 5 parts, magnesium sulfate.
The herbal medicine of the present embodiment and the composite biological agent result of use of marine alga as follows:
Subjects: plastic tent cucumber, experiment field and contrast each 1 mu of field;
Experimental plot: the composite biological agent 10kg executing herbal medicine and marine alga with the form of topdressing every mu punching;
Contrast field: execute nitrogen, phosphorus, potassium (20wt%-20wt%-20wt%) macroelement Water soluble fertilizer 10kg with the form of topdressing every mu punching;
Contrast effect: punching execute nitrogen, phosphorus, potassium (15wt%-15wt%-15wt%) macroelement Water soluble fertilizer contrast field root knot nematode evil serious, yellow leaf, sugar-preserved gourd dysplasia phenomenon is serious; The experiment field that TCM Algae fertilizer is executed in punching is substantially by insect pest leaf look not dark green, and sugar-preserved gourd is straight, and color is good, and experiment field is to high by about 21% according to real estate amount.
Embodiment 2
The herbal medicine of the present embodiment and the composite biological agent of marine alga, comprise following component, and its weight proportion is:
Herbal medicine marine alga mixing base fluid 20 parts, humic acids 5 parts, 5 parts, urea, monoammonium phosphate 10 parts, potassium primary phosphate 5 parts, potassium sulfate 10 parts, chelated iron 5 parts, 5 parts, zinc sulfate;
Wherein, herbal medicine marine alga mixing base fluid comprises following component, and its weight proportion is:
Extracts of Chinese herbal medicine 45 parts, seaweed extracted liquor 60 parts, buffer reagent metalloprotease 5 parts, 5 parts, stablizer wood vinegar;
The main raw material weight proportion of extracts of Chinese herbal medicine is as follows: tuber of stemona 50g, bark of eucommia 20g, root of langdu 50g, Herba Andrographis 30g, Herba Sophorae alopecuroidis 10g, Radix Astragali 50g, root of large-flowered skullcap 40g, Herba Houttuyniae 90g, kuh-seng 10g, Radix Glycyrrhizae 30g, garlicin 30g, pipe tobacco 80g, Rangooncreeper Fruit 30g;
The main raw material of seaweed extracted liquor is brown alga.
The preparation method of the herbal medicine of the present embodiment and the composite biological agent of marine alga, comprises following content:
The first step, produce extracts of Chinese herbal medicine: mixing after being cleaned by the herbal raw material of formula ratio, is crushed to 300 orders, extracts by following technique:
1, mixed with water by herbal powder, water consumption is 15 times of herbal powder weight, adds 50mol/L sulfuric acid and pH value is adjusted to 3, adds Sumizyme MP 20 degree of extractions Celsius 30 hours;
2, cool to about 0 degree, add 60mol/L sodium hydroxide and pH value is adjusted to 7, be separated and remove residue, obtain tawny liquid, then vacuum pressed is concentrated into proportion is that namely 0.8g/mL obtains extracts of Chinese herbal medicine;
Wherein, the weight proportion of herbal raw material each component is: tuber of stemona 50g, bark of eucommia 20g, root of langdu 50g, Herba Andrographis 30g, Herba Sophorae alopecuroidis 10g, Radix Astragali 50g, root of large-flowered skullcap 40g, Herba Houttuyniae 90g, kuh-seng 10g, Radix Glycyrrhizae 30g, garlicin 30g, pipe tobacco 80g, Rangooncreeper Fruit 30g.
Second step, produce seaweed extracted liquor: marine alga is cleaned ovendry power and be broken to 300 orders, extract by following technique:
1, mixed with water by marine algae powder, water consumption is 15 times of marine algae powder weight, adds 50mol/L sulfuric acid and pH value is adjusted to 1, extracts 4 hours at 50 degrees Celsius;
2, add 60mol/L sodium hydroxide and pH value is adjusted to 7, then separating and removing impurities;
3, vacuum pressed is concentrated into proportion is 1g/mL, namely obtains seaweed extracted liquor.
3rd step, produces herbal medicine marine alga mixing base fluid: fully mixed with seaweed extracted liquor by extracts of Chinese herbal medicine, then add buffer reagent metalloprotease, stablizer wood vinegar;
The weight proportion of each component is: extracts of Chinese herbal medicine 45 parts, seaweed extracted liquor 60 parts, buffer reagent metalloprotease 5 parts, 5 parts, stablizer wood vinegar.
4th step, makes the composite biological agent of herbal medicine and marine alga: add humic acids, urea, monoammonium phosphate, potassium primary phosphate, potassium sulfate and magnesium by herbal medicine marine alga mixing base fluid, mix;
The weight proportion of each component is: herbal medicine marine alga mixing base fluid 20 parts, humic acids 5 parts, 5 parts, urea, monoammonium phosphate 10 parts, potassium primary phosphate 5 parts, potassium sulfate 10 parts, chelated iron 5 parts, 5 parts, zinc sulfate.
The herbal medicine of the present embodiment and the composite biological agent result of use of marine alga as follows:
Subjects: booth sponge gourd, experiment field and contrast each 1 mu of field;
Experimental plot: the composite biological agent 10kg executing herbal medicine and marine alga with the form of topdressing every mu punching;
Contrast field: execute nitrogen, phosphorus, potassium (20wt%-20wt%-20wt%) macroelement Water soluble fertilizer 10kg with the form of topdressing every mu punching;
Contrast effect: punching execute nitrogen, phosphorus, potassium (20wt%-20wt%-20wt%) macroelement Water soluble fertilizer contrast field root knot nematode evil serious, yellow leaf, sugar-preserved gourd eel-like figure phenomenon is serious, and bacterial disease is also heavier; Punching executes the experiment field of TCM Algae fertilizer substantially not by insect pest, and blade is plump, and sugar-preserved gourd is straight and long, is beautiful in design and color, and does not substantially have the disease of leaf class, and experiment field is to high by about 23% according to real estate amount.
Embodiment 3
The herbal medicine of the present embodiment and the composite biological agent of marine alga, comprise following component, and its weight proportion is:
Herbal medicine marine alga mixing base fluid 40 parts, humic acids 15 parts, 15 parts, urea, monoammonium phosphate 15 parts, potassium primary phosphate 15 parts, potassium sulfate 15 parts, 3 parts, copper sulfate, manganous sulfate 3 parts, ammonium molybdate 2 parts, 7 parts, zinc sulfate;
Wherein, herbal medicine marine alga mixing base fluid comprises following component, and its weight proportion is:
Extracts of Chinese herbal medicine 20 parts, seaweed extracted liquor 55 parts, buffer reagent metalloprotease 10 parts, 15 parts, stablizer wood vinegar;
The main raw material weight proportion of extracts of Chinese herbal medicine is as follows: tuber of stemona 40g, bark of eucommia 20g, root of langdu 50g, Herba Andrographis 50g, Herba Sophorae alopecuroidis 30g, Radix Astragali 40g, root of large-flowered skullcap 50g, Herba Houttuyniae 120g, kuh-seng 30g, Radix Glycyrrhizae 40g, garlicin 60g, pipe tobacco 100g, Rangooncreeper Fruit 70g;
The main raw material of seaweed extracted liquor is brown alga.
The preparation method of the herbal medicine of the present embodiment and the composite biological agent of marine alga, comprises following content:
The first step, produce extracts of Chinese herbal medicine: mixing after being cleaned by the herbal raw material of formula ratio, is crushed to 300 orders, extracts by following technique:
1, mixed with water by herbal powder, water consumption is 20 times of herbal powder weight, adds 40mol/L sulfuric acid and pH value is adjusted to 1, adds Sumizyme MP 25 degree of extractions Celsius 30 hours;
2, cool to about 0 degree, add 60mol/L salt of wormwood and pH value is adjusted to 7, be separated and remove residue, obtain tawny liquid, then vacuum pressed is concentrated into proportion is that namely 1.2g/mL obtains extracts of Chinese herbal medicine;
Wherein, the weight proportion of herbal raw material each component is: tuber of stemona 40g, bark of eucommia 20g, root of langdu 50g, Herba Andrographis 50g, Herba Sophorae alopecuroidis 30g, Radix Astragali 40g, root of large-flowered skullcap 50g, Herba Houttuyniae 120g, kuh-seng 30g, Radix Glycyrrhizae 40g, garlicin 60g, pipe tobacco 100g, Rangooncreeper Fruit 70g.
Second step, produce seaweed extracted liquor: marine alga is cleaned ovendry power and be broken to 300 orders, extract by following technique:
1, mixed with water by marine algae powder, water consumption is 10 times of marine algae powder weight, adds 60mol/L salt of wormwood and pH value is adjusted to 9, extracts 7 hours at 75 degrees Celsius;
2, add 40mol/L sulfuric acid and pH value is adjusted to 7, then separating and removing impurities;
3, vacuum pressed is concentrated into proportion is 1.4g/mL, namely obtains seaweed extracted liquor.
3rd step, produces herbal medicine marine alga mixing base fluid: fully mixed with seaweed extracted liquor by extracts of Chinese herbal medicine, then add buffer reagent metalloprotease, stablizer wood vinegar;
The weight proportion of each component is: extracts of Chinese herbal medicine 20 parts, seaweed extracted liquor 55 parts, buffer reagent metalloprotease 10 parts, 15 parts, stablizer wood vinegar.
4th step, makes the composite biological agent of herbal medicine and marine alga: add humic acids, urea, monoammonium phosphate, potassium primary phosphate, potassium sulfate and magnesium by herbal medicine marine alga mixing base fluid, mix;
The weight proportion of each component is: herbal medicine marine alga mixing base fluid 40 parts, humic acids 15 parts, 15 parts, urea, monoammonium phosphate 15 parts, potassium primary phosphate 15 parts, potassium sulfate 15 parts, 3 parts, copper sulfate, manganous sulfate 3 parts, ammonium molybdate 2 parts, 7 parts, zinc sulfate.
The herbal medicine of the present embodiment and the composite biological agent result of use of marine alga as follows:
Subjects: shed capsicum, experiment field and contrast each 1 mu of field;
Experimental plot: the composite biological agent 10kg executing herbal medicine and marine alga with the form of topdressing every mu punching;
Contrast field: execute nitrogen, phosphorus, potassium (14wt%-6wt%-30wt%) macroelement Water soluble fertilizer 10kg with the form of topdressing every mu punching;
Contrast effect: punching execute nitrogen, phosphorus, potassium (14wt%-6wt%-30wt%) macroelement Water soluble fertilizer the root knot nematode insect pest of contrast field heavier, sit flower rate few, trialeurodes vaporariorum is more, punching executes the experiment field of TCM Algae fertilizer substantially not by insect pest, leaf look dark green, and pepper fruit is large, and fruit type is straight, substantially do not have trialeurodes vaporariorum to occur, experiment field is to high by about 19% according to real estate amount.
Embodiment 4
The herbal medicine of the present embodiment and the composite biological agent of marine alga, comprise following component, and its weight proportion is:
Herbal medicine marine alga mixing base fluid 50 parts, humic acids 25 parts, 20 parts, urea, monoammonium phosphate 20 parts, potassium primary phosphate 20 parts, potassium sulfate 2 parts, 5 parts, magnesium sulfate, 3 parts, copper sulfate, manganous sulfate 3 parts, chelated iron 3 parts, boron 3 parts, 3 parts, nitrocalcite;
Wherein, herbal medicine marine alga mixing base fluid comprises following component, and its weight proportion is:
Extracts of Chinese herbal medicine 25 parts, seaweed extracted liquor 50 parts, buffer reagent metalloprotease 10 parts, 15 parts, stablizer wood vinegar;
The main raw material weight proportion of extracts of Chinese herbal medicine is as follows: hundred step 60g, bark of eucommia 50g, root of langdu 60g, Herba Andrographis 30g, Herba Sophorae alopecuroidis 10g, Radix Astragali 60g, root of large-flowered skullcap 60g, Herba Houttuyniae 130g, kuh-seng 40g, Radix Glycyrrhizae 20g, garlicin 50g, pipe tobacco 130g, Rangooncreeper Fruit 30g;
The main raw material of seaweed extracted liquor is brown alga.
The preparation method of the herbal medicine of the present embodiment and the composite biological agent of marine alga, comprises following content:
The first step, produce extracts of Chinese herbal medicine: mixing after being cleaned by the herbal raw material of formula ratio, is crushed to 300 orders, extracts by following technique:
1, mixed with water by herbal powder, water consumption is 20 times of herbal powder weight, adds 50mol/L potassium hydroxide and pH value is adjusted to 11, adds Sumizyme MP 45 degree of extractions Celsius 24 hours;
2, cool to about 0 degree, add 40mol/L hydrochloric acid and pH value is adjusted to 7, be separated and remove residue, obtain tawny liquid, then vacuum pressed is concentrated into proportion is that namely 0.9g/mL obtains extracts of Chinese herbal medicine;
Wherein, the weight proportion of herbal raw material each component is: tuber of stemona 60g, bark of eucommia 50g, root of langdu 60g, Herba Andrographis 30g, Herba Sophorae alopecuroidis 10g, Radix Astragali 60g, root of large-flowered skullcap 60g, Herba Houttuyniae 130g, kuh-seng 40g, Radix Glycyrrhizae 20g, garlicin 50g, pipe tobacco 130g, Rangooncreeper Fruit 30g.
Second step, produce seaweed extracted liquor: marine alga is cleaned ovendry power and be broken to 300 orders, extract by following technique:
1, mixed with water by marine algae powder, water consumption is 20 times of marine algae powder weight, adds 50mol/L potassium hydroxide and pH value is adjusted to 11, extracts 4 hours at 100 degrees Celsius;
2, add 40mol/L hydrochloric acid and pH value is adjusted to 7, then separating and removing impurities;
3, vacuum pressed is concentrated into proportion is 1.2g/mL, namely obtains seaweed extracted liquor.
3rd step, produces herbal medicine marine alga mixing base fluid: fully mixed with seaweed extracted liquor by extracts of Chinese herbal medicine, then add buffer reagent metalloprotease, stablizer wood vinegar;
The weight proportion of each component is: extracts of Chinese herbal medicine 25 parts, seaweed extracted liquor 50 parts, buffer reagent metalloprotease 10 parts, 15 parts, stablizer wood vinegar.
4th step, makes the composite biological agent of herbal medicine and marine alga: add humic acids, urea, monoammonium phosphate, potassium primary phosphate, potassium sulfate and magnesium by herbal medicine marine alga mixing base fluid, mix;
The weight proportion of each component is: herbal medicine marine alga mixing base fluid 50 parts, humic acids 25 parts, 20 parts, urea, monoammonium phosphate 20 parts, potassium primary phosphate 20 parts, potassium sulfate 2 parts, 5 parts, magnesium sulfate, 3 parts, copper sulfate, manganous sulfate 3 parts, chelated iron 3 parts, boron 3 parts, 3 parts, nitrocalcite.
The herbal medicine of the present embodiment and the composite biological agent result of use of marine alga as follows:
Subjects: shed leek (only son and heir is red), experiment field and contrast each 1 mu of field;
Experimental plot: the composite biological agent 10kg executing herbal medicine and marine alga with the form of topdressing every mu punching;
Contrast field: execute nitrogen, phosphorus, potassium (14wt%-6wt%-30wt%) macroelement Water soluble fertilizer 10kg with the form of topdressing every mu punching;
Contrast effect: punching execute nitrogen, phosphorus, potassium (14wt%-6wt%-30wt%) macroelement Water soluble fertilizer contrast field fragrant-flowered garlic maggot sting that to bite phenomenon heavier, stem is thin and have dry sharp phenomenon, and the experiment field that TCM Algae fertilizer is executed in punching does not have insect pest substantially, and leaf look blackish green, stem is sturdy; Quality of vegetable is excellent, and contrast contrast single batch, field, experiment field income is high by more than 30%.
Embodiment 5
The herbal medicine of the present embodiment and the composite biological agent of marine alga, comprise following component, and its weight proportion is:
Herbal medicine marine alga mixing base fluid 70 parts, humic acids 20 parts, 25 parts, urea, monoammonium phosphate 25 parts, potassium primary phosphate 25 parts, potassium sulfate 30 parts, 2 parts, magnesium;
Wherein, herbal medicine marine alga mixing base fluid comprises following component, and its weight proportion is:
Extracts of Chinese herbal medicine 30 parts, seaweed extracted liquor 80 parts, buffer reagent metalloprotease 20 parts, 20 parts, stablizer wood vinegar;
The main raw material weight proportion of extracts of Chinese herbal medicine is as follows: tuber of stemona 70g, bark of eucommia 70g, root of langdu 80g, Herba Andrographis 70g, Herba Sophorae alopecuroidis 40g, Radix Astragali 80g, root of large-flowered skullcap 40g, Herba Houttuyniae 100g, kuh-seng 10g, Radix Glycyrrhizae 50g, garlicin 40g, pipe tobacco 150g, Rangooncreeper Fruit 60g;
The main raw material of seaweed extracted liquor is brown alga.
The preparation method of the herbal medicine of the present embodiment and the composite biological agent of marine alga, comprises following content:
The first step, produce extracts of Chinese herbal medicine: mixing after being cleaned by the herbal raw material of formula ratio, is crushed to 300 orders, extracts by following technique:
1, mixed with water by herbal powder, water consumption is 10 times of herbal powder weight, adds 30mol/L phosphoric acid and pH value is adjusted to 3, adds Sumizyme MP 15 degree of extractions Celsius 30 hours;
2, cool to about 0 degree, add 50mol/L sodium carbonate and pH value is adjusted to 7, be separated and remove residue, obtain tawny liquid, then vacuum pressed is concentrated into proportion is that namely 0.8g/mL obtains extracts of Chinese herbal medicine;
Wherein, the weight proportion of herbal raw material each component is: tuber of stemona 70g, bark of eucommia 70g, root of langdu 80g, Herba Andrographis 70g, Herba Sophorae alopecuroidis 40g, Radix Astragali 80g, root of large-flowered skullcap 40g, Herba Houttuyniae 100g, kuh-seng 10g, Radix Glycyrrhizae 50g, garlicin 40g, pipe tobacco 150g, Rangooncreeper Fruit 70g.
Second step, produce seaweed extracted liquor: marine alga is cleaned ovendry power and be broken to 300 orders, extract by following technique:
1, mixed with water by marine algae powder, water consumption is 15 times of marine algae powder weight, adds 30mol/L phosphoric acid and pH value is adjusted to 3, extracts 5 hours at 45 degrees Celsius;
2, add 50mol/L sodium carbonate and pH value is adjusted to 7, then separating and removing impurities;
3, vacuum pressed is concentrated into proportion is 1.2g/mL, namely obtains seaweed extracted liquor.
3rd step, produces herbal medicine marine alga mixing base fluid: fully mixed with seaweed extracted liquor by extracts of Chinese herbal medicine, then add buffer reagent metalloprotease, stablizer wood vinegar;
The weight proportion of each component is: extracts of Chinese herbal medicine 30 parts, seaweed extracted liquor 80 parts, buffer reagent metalloprotease 20 parts, 20 parts, stablizer wood vinegar;
4th step, makes the composite biological agent of herbal medicine and marine alga: add humic acids, urea, monoammonium phosphate, potassium primary phosphate, potassium sulfate and magnesium by herbal medicine marine alga mixing base fluid, mix;
The weight proportion of each component is: herbal medicine marine alga mixing base fluid 70 parts, humic acids 20 parts, 25 parts, urea, monoammonium phosphate 25 parts, potassium primary phosphate 25 parts, potassium sulfate 30 parts, 2 parts, magnesium sulfate.
The herbal medicine of the present embodiment and the composite biological agent result of use of marine alga as follows:
Subjects: shed great Jiang, experiment field and contrast each 1 mu of field;
Experimental plot: the composite biological agent 10kg executing herbal medicine and marine alga with the form of topdressing every mu punching;
Contrast field: execute nitrogen, phosphorus, potassium (18wt%-7wt%-30wt%) macroelement Water soluble fertilizer 10kg with the form of topdressing every mu punching;
Contrast effect: punching execute nitrogen, phosphorus, potassium (18wt%-7wt%-30wt%) macroelement Water soluble fertilizer contrast field shameless sick heavier, wilt, dry sharp phenomenon be serious; The experiment field that TCM Algae fertilizer is executed in punching does not have shameless sick phenomenon to occur substantially; The growth of leaf look dark green is vigorous, and bacterial wilt of ginger is also than contrast field few 70%, and experiment field is to high by about 26% according to real estate amount.
Embodiment 6
The herbal medicine of the present embodiment and the composite biological agent of marine alga, comprise following component, and its weight proportion is:
Herbal medicine marine alga mixing base fluid 80 parts, humic acids 5 parts, 5 parts, urea, monoammonium phosphate 20 parts, potassium primary phosphate 5 parts, potassium sulfate 2 parts, 5 parts, magnesium sulfate, 3 parts, copper sulfate, manganous sulfate 3 parts, 3 parts, nitrocalcite;
Wherein, herbal medicine marine alga mixing base fluid comprises following component, and its weight proportion is:
Extracts of Chinese herbal medicine 35 parts, seaweed extracted liquor 50 parts, buffer reagent metalloprotease 5 parts, 10 parts, stablizer wood vinegar;
The main raw material weight proportion of extracts of Chinese herbal medicine is as follows: tuber of stemona 60g, bark of eucommia 50g, root of langdu 50g, Herba Andrographis 30g, Herba Sophorae alopecuroidis 10g, Radix Astragali 50g, root of large-flowered skullcap 40g, Herba Houttuyniae 130g, kuh-seng 40g, Radix Glycyrrhizae 20g, garlicin 50g, pipe tobacco 100g, Rangooncreeper Fruit 20g;
The main raw material of seaweed extracted liquor is brown alga.
The preparation method of the herbal medicine of the present embodiment and the composite biological agent of marine alga, comprises following content:
The first step, produce extracts of Chinese herbal medicine: mixing after being cleaned by the herbal raw material of formula ratio, is crushed to 300 orders, extracts by following technique:
1, mixed with water by herbal powder, water consumption is 10 times of herbal powder weight, adds 40mol/L potassium hydroxide and pH value is adjusted to 10, adds Sumizyme MP 65 degree of extractions Celsius 24 hours;
2, cool to about 0 degree, add 40mo1/L hydrochloric acid and pH value is adjusted to 7, be separated and remove residue, obtain tawny liquid, then vacuum pressed is concentrated into proportion is that namely 1.1g/mL obtains extracts of Chinese herbal medicine;
Wherein, the weight proportion of herbal raw material each component is: tuber of stemona 60g, bark of eucommia 50g, root of langdu 50g, Herba Andrographis 30g, Herba Sophorae alopecuroidis 10g, Radix Astragali 50g, root of large-flowered skullcap 40g, Herba Houttuyniae 130g, kuh-seng 40g, Radix Glycyrrhizae 20g, garlicin 50g, pipe tobacco 100g, Rangooncreeper Fruit 20g.
Second step, produce seaweed extracted liquor: marine alga is cleaned ovendry power and be broken to 300 orders, extract by following technique:
1, mixed with water by marine algae powder, water consumption is 20 times of marine algae powder weight, adds 40mol/L hydrochloric acid and pH value is adjusted to 3, extracts 3 hours at 65 degrees Celsius;
2, add 40mol/L potassium hydroxide and pH value is adjusted to 7, then separating and removing impurities;
3, vacuum pressed is concentrated into proportion is 1.2g/mL, namely obtains seaweed extracted liquor.
3rd step, produces herbal medicine marine alga mixing base fluid: fully mixed with seaweed extracted liquor by extracts of Chinese herbal medicine, then add buffer reagent metalloprotease, stablizer wood vinegar;
The weight proportion of each component is: extracts of Chinese herbal medicine 35 parts, seaweed extracted liquor 50 parts, buffer reagent metalloprotease 5 parts, 10 parts, stablizer wood vinegar.
4th step, makes the composite biological agent of herbal medicine and marine alga: add humic acids, urea, monoammonium phosphate, potassium primary phosphate, potassium sulfate and magnesium by herbal medicine marine alga mixing base fluid, mix;
The weight proportion of each component is: herbal medicine marine alga mixing base fluid 80 parts, humic acids 5 parts, 5 parts, urea, monoammonium phosphate 20 parts, potassium primary phosphate 5 parts, potassium sulfate 2 parts, 5 parts, magnesium sulfate, 3 parts, copper sulfate, manganous sulfate 3 parts, 3 parts, nitrocalcite.
The above is only to preferred embodiment of the present invention, and be not do other forms of restriction to the present invention, any those skilled in the art may utilize the technology contents of above-mentioned announcement to be changed or be modified as the Equivalent embodiments of equivalent variations.But, everyly do not depart from the present invention program's content, according to technical spirit of the present invention to any simple modification made for any of the above embodiments, equivalent variations and remodeling, still belong to protection scope of the present invention.

Claims (9)

1. a composite biological agent for herbal medicine and marine alga, is characterized in that: comprise following component, and its weight proportion is:
Herbal medicine marine alga mixing base fluid 10 parts-80 parts, humic acids 5 parts-25 parts, 5 parts-25 parts, urea, monoammonium phosphate 5 parts-25 parts, potassium primary phosphate 5 parts-25 parts, potassium sulfate 2 parts-30 parts, trace element 2 parts-20 parts;
Wherein, herbal medicine marine alga mixing base fluid comprises following component, and its weight proportion is:
Extracts of Chinese herbal medicine 20 parts-45 parts, seaweed extracted liquor 50 parts-80 parts, buffer reagent metalloprotease 5 parts-20 parts, 5 parts-20 parts, stablizer wood vinegar;
Wherein, the main raw material weight proportion of extracts of Chinese herbal medicine is as follows: tuber of stemona 40-70g, bark of eucommia 20-70g, root of langdu 30-80g, Herba Andrographis 30-70g, Herba Sophorae alopecuroidis 10-40g, Radix Astragali 30-80g, root of large-flowered skullcap 20-60g, Herba Houttuyniae 90-130g, kuh-seng 10-40g, Radix Glycyrrhizae 20-50g, garlicin 30-60g, pipe tobacco 80-150g, Rangooncreeper Fruit 20-70g;
Its preparation method comprises the following steps:
The first step, produces extracts of Chinese herbal medicine: mixing after being cleaned by the herbal raw material of formula ratio, is crushed to 300 orders, extracts by following (1), (3) or (2), (3) two kinds of techniques:
(1) herbal powder is mixed with water, pH value is adjusted to 9-11, add Sumizyme MP and extract 24 hours at 45-65 degree Celsius;
(2) herbal powder is mixed with water, pH value is adjusted to 1-3, add aspartic protease and extract 30 hours at 15-25 degree Celsius;
(3) cool to about 0 degree, pH value is adjusted to 7, be separated and remove residue, then vacuum pressed is concentrated into proportion is that namely 0.8-1.2g/mL obtains extracts of Chinese herbal medicine;
Second step, produces seaweed extracted liquor: marine alga is cleaned ovendry power and be broken to 300 orders, extracts by (1), (3), (4) or (2), (3), (4) two kinds of techniques:
(1) marine algae powder is mixed with water, pH value is adjusted to 9-11, extract 4-7 hour at 75-100 degree Celsius;
(2) marine algae powder is mixed with water, pH value is adjusted to 1-3, extract 3-5 hour at 45-65 degree Celsius;
(3) pH value is adjusted to 7, then separating and removing impurities;
(4) vacuum pressed is concentrated into proportion is 1-1.4g/mL, namely obtains seaweed extracted liquor;
3rd step, produces herbal medicine marine alga mixing base fluid: fully mixed with seaweed extracted liquor by extracts of Chinese herbal medicine, then add buffer reagent metalloprotease, with stablizer wood vinegar;
4th step, makes the composite biological agent of herbal medicine and marine alga: add humic acids, urea, potassium sulfate, monoammonium phosphate, potassium primary phosphate and trace element by herbal medicine marine alga mixing base fluid, mix.
2. the composite biological agent of herbal medicine according to claim 1 and marine alga, is characterized in that: the main raw material of seaweed extracted liquor is brown alga.
3. the composite biological agent of herbal medicine according to claim 1 and marine alga, is characterized in that: trace element is one or more in magnesium, copper, manganese, iron, boron, zinc, molybdenum, calcium.
4. a preparation method for the composite biological agent of herbal medicine according to claim 1 and marine alga, is characterized in that: comprise following content:
The first step, produces extracts of Chinese herbal medicine: mixing after being cleaned by the herbal raw material of formula ratio, is crushed to 300 orders, extracts by following (1), (3) or (2), (3) two kinds of techniques:
(1) herbal powder is mixed with water, pH value is adjusted to 9-11, add Sumizyme MP and extract 24 hours at 45-65 degree Celsius;
(2) herbal powder is mixed with water, pH value is adjusted to 1-3, add aspartic protease and extract 30 hours at 15-25 degree Celsius;
(3) cool to about 0 degree, pH value is adjusted to 7, be separated and remove residue, then vacuum pressed is concentrated into proportion is that namely 0.8-1.2g/mL obtains extracts of Chinese herbal medicine;
The weight proportion of described herbal raw material each component is: tuber of stemona 40-70g, bark of eucommia 20-70g, root of langdu 30-80g, Herba Andrographis 30-70g, Herba Sophorae alopecuroidis 10-40g, Radix Astragali 30-80g, root of large-flowered skullcap 20-60g, Herba Houttuyniae 90-130g, kuh-seng 10-40g, Radix Glycyrrhizae 20-50g, garlicin 30-60g, pipe tobacco 80-150g, Rangooncreeper Fruit 20-70g;
Second step, produces seaweed extracted liquor: marine alga is cleaned ovendry power and be broken to 300 orders, extracts by (1), (3), (4) or (2), (3), (4) two kinds of techniques:
(1) marine algae powder is mixed with water, pH value is adjusted to 9-11, extract 4-7 hour at 75-100 degree Celsius;
(2) marine algae powder is mixed with water, pH value is adjusted to 1-3, extract 3-5 hour at 45-65 degree Celsius;
(3) pH value is adjusted to 7, then separating and removing impurities;
(4) vacuum pressed is concentrated into proportion is 1-1.4g/mL, namely obtains seaweed extracted liquor;
3rd step, produces herbal medicine marine alga mixing base fluid: fully mixed with seaweed extracted liquor by extracts of Chinese herbal medicine, then add buffer reagent metalloprotease, with stablizer wood vinegar;
The weight proportion of described each component is: extracts of Chinese herbal medicine 20 parts-45 parts, seaweed extracted liquor 60 parts-80 parts, buffer reagent metalloprotease 5 parts-20 parts, 5 parts-20 parts, stablizer wood vinegar;
4th step, makes the composite biological agent of herbal medicine and marine alga: add humic acids, urea, potassium sulfate, monoammonium phosphate, potassium primary phosphate and trace element by herbal medicine marine alga mixing base fluid, mix;
The weight proportion of described each component is: herbal medicine marine alga mixing base fluid 10 parts-80 parts, humic acids 5 parts-25 parts, 5 parts-25 parts, urea, potassium sulfate 2 parts-30 parts, monoammonium phosphate 5 parts-25 parts, potassium primary phosphate 5 parts-25 parts, trace element 2 parts-20 parts.
5. the preparation method of the composite biological agent of herbal medicine according to claim 4 and marine alga, is characterized in that: the first step, produces in extracts of Chinese herbal medicine, and the water consumption in (1) or (2) is 10-20 times of herbal powder weight.
6. the preparation method of the composite biological agent of herbal medicine according to claim 4 and marine alga, is characterized in that: second step, produces in seaweed extracted liquor, and the water consumption in (1) or (2) is 10-20 times of marine algae powder weight.
7. the preparation method of the composite biological agent of herbal medicine according to claim 4 and marine alga, it is characterized in that: the first step, produce in extracts of Chinese herbal medicine, the alkali that adjust ph uses be potassium hydroxide, sodium hydroxide, salt of wormwood or sodium carbonate, the acid of use is hydrochloric acid, sulfuric acid or phosphoric acid.
8. the preparation method of the composite biological agent of herbal medicine according to claim 4 and marine alga, it is characterized in that: second step, produce in seaweed extracted liquor, the alkali that adjust ph uses be potassium hydroxide, sodium hydroxide, salt of wormwood or sodium carbonate, the acid of use is hydrochloric acid, sulfuric acid or phosphoric acid.
9. the preparation method of the composite biological agent of herbal medicine according to claim 4 and marine alga, is characterized in that: described trace element is one or more in magnesium, copper, manganese, iron, boron, zinc, molybdenum, calcium.
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