CN102771277A - Technical method for nonwoven fabric covering during tissue culture seedling transplanting - Google Patents
Technical method for nonwoven fabric covering during tissue culture seedling transplanting Download PDFInfo
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- CN102771277A CN102771277A CN2012102352491A CN201210235249A CN102771277A CN 102771277 A CN102771277 A CN 102771277A CN 2012102352491 A CN2012102352491 A CN 2012102352491A CN 201210235249 A CN201210235249 A CN 201210235249A CN 102771277 A CN102771277 A CN 102771277A
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- nonwoven
- seedling
- transplanting
- tissue cultivating
- tissue culture
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Abstract
The invention relates to a technical method for nonwoven fabric covering during tissue culture seedling transplanting. The method comprises several steps including seedling bed building, substrate selection, tissue culture seedling clean washing, tissue culture seedling transplanting and management after the transplanting. The technical method for nonwoven fabric covering during tissue culture seedling transplanting overcomes the defects of plastic agricultural films, the time and the labor are saved, the ventilation, the light transmission and the moisture preservation are realized, in addition, the seedling bed and the non-woven fabric can form the rectangular space, the temperature is stable, the temperature fluctuation is avoided, and the method perfectly solves the problems to realize the effects that certain light illumination is needed by seedling growth, higher air humidity is required during the transplanting, in addition, the excessive substrate humidity is avoided, and the condition is very favorable for the transplanting survival of the tissue culture seedling.
Description
Technical field
The present invention relates to a kind of tissue cultivating seedling and transplant the technical method that covers nonwoven.
Background technology
The plant tissue culture technology is to be the technology that base growth is got up 20 beginnings of the century with plant physiology; It is widely used in quick breeding, removes virus, quickens each side such as breeding process and germ plasm resource preservation, has all obtained huge economic benefit, social benefit and ecological benefits.
Production practices prove that group is trained the height of the transplantation of seedlings survival rate of taking root, and are determining the height of group training unit cost production.Because of tissue cultivating seedling is in aseptic, nutritious supply, suitable temperature, illumination and 100% relative moisture environment, to grow; And have suitable plant hormone to regulate physiological requirements such as growth metabolism, in case the bottle outlet plantation, the acute variation of its growth has taken place to be unfavorable in environment; No longer can keep 100% easily such as humidity; Temperature does not have culturing room such suitable yet, has lost nutritional support, transplants the environment flora of possibly mixing and gives birth to or the like.In a word, so tender and lovely seedling is to be difficult to plant live, and is careless slightly, just can cause a large amount of dead seedlings, and the experience of the cost of making it over is irrevocably lost.In order to keep humidity common plastics plastic sheeting for farm use on the seedbed to cover, though the plastic film good permeability, moisture retention is good in the production, stuffiness, and the temperature under covering is unstable.The space temperature that plastic film forms under solar light irradiation sharply rises, and is unfavorable for the growth of tissue cultivating seedling.Be to guarantee that air humidity needs often spraying, but after the spraying how, water supply in media increases air and reduces, cause the root anoxic and seedling death phenomenon takes place.So printing opacity, ventilating, preserving moisture is difficult between this three coordinate all the time, often causes a large amount of dead seedlings, has increased the cost of tissue cultivating seedling.
Summary of the invention
The objective of the invention is to overcome above-mentioned deficiency, provide a kind of tissue cultivating seedling to transplant the technical method that covers nonwoven, the environment that makes the seedling after the transplanting keep a printing opacity, ventilate and preserve moisture has guaranteed the survival rate of transplanted seedling.
The objective of the invention is to realize like this:
A kind of tissue cultivating seedling is transplanted the technical method that covers nonwoven, and said technical method comprises following processing step:
Build in step 1, seedbed
On the seedbed, build a framework, cover at framework external application nonwoven then, make it to form the space of a seedbed and nonwoven formation;
Step 2, matrix are selected
Step 3, tissue cultivating seedling are cleaned
Clean tissue cultivating seedling root agar;
Step 4, tissue cultivating seedling are transplanted
In the booth that shelters from heat or light, contain the tissue cultivating seedling immigration in the seedling dish of matrix, spray liquid medicine, put again in the seedbed of the described nonwoven covering of step 1;
Management after step 5, the transplanting
At first, keep the moistening of nonwoven;
Secondly, every 5-7 days, raise nonwoven and spray diseases prevention once with liquid medicine.
A kind of tissue cultivating seedling of the present invention is transplanted the technical method that covers nonwoven; Matrix is selected turfy soil, perlite and the vermiculite mixed with 1:1:1 or 2:2:1, and adding the mass concentration that 50% carbendazol wettable powder processes then is that millesimal carbendazim solution drenches.
A kind of tissue cultivating seedling of the present invention is transplanted the technical method that covers nonwoven, and liquid medicine is that the mass concentration that 50% carbendazol wettable powder is processed is millesimal carbendazim solution.
Compared with prior art, the invention has the beneficial effects as follows:
A kind of tissue cultivating seedling of the present invention is transplanted the technical method that covers nonwoven and has been overcome the drawback with plastic film; Save time and laborsaving, both ventilation, printing opacity, preserve moisture and to make seedbed and nonwoven form the cuboid space temperature steady, unlikely not high not low; Solved the certain illumination of seedling growth needs preferably; Need the higher humidity of air when just transplanting, and unlikely soil humidity is excessive, is very beneficial for the transplanting survival of tissue cultivating seedling.
Embodiment
A kind of tissue cultivating seedling of the present invention is transplanted the technical method that covers nonwoven, comprises following processing step:
Build in step 1, seedbed
1, selects 50g/m for use
2The nonwoven of fabric width 2450mm;
2, on the seedbed, build a high 0.3m, wide 1.7m with batten or bamboo chip, length is fixed cuboid framework according to booth length, and adds a crosspiece at framework interval 0.7m;
3, cover on the cuboid framework with 1 described nonwoven, nonwoven length and width four limits are hidden into the bottom, seedbed, make it to form a cuboid space.
Step 2, matrix are selected
Select turfy soil, perlite and vermiculite mixed with 1:1:1 or 2:2:1; Adding the mass concentration that 50% carbendazol wettable powder processes then is that millesimal carbendazim solution drenches; Do not drip to hold the agglomerating webs of matrix in hand; And flat breastheight degree falls down to scatter and is degree, in 72 holes or the 56 holes dish of packing into;
Step 3, tissue cultivating seedling are cleaned
Clean tissue cultivating seedling root agar, taking-up group is gently trained the seedling of taking root, and cleans root agar then, and by large, medium and small classification; In the booth that shelters from heat or light,, spray liquid medicine,
Step 4, tissue cultivating seedling are transplanted
In the booth that shelters from heat or light; Move into tissue cultivating seedling that every cave plants a young plant in the seedling dish contain matrix; Having planted a dish mass percent of drenching immediately is that millesimal 50% carbendazol wettable powder solution is made normal root water, puts in the seedbed that the described nonwoven of step 1 covers again.
Management after step 5, the transplanting
1, keep nonwoven moistening with the degree of being that do not drip, nonwoven is done i.e. water spray, in the micropore of nonwoven, forms moisture film, makes through water evaporates in the cuboid space of seedling dish and nonwoven formation, and humidity remains on 95-100%, is very beneficial for the growth of seedling.
2, every 5-7 days, raise mass concentration that nonwoven uses 50% carbendazol wettable powder to process and be the diseases prevention of millesimal carbendazim solution spray medicine once, general logotype 3 times, disease-free basically generation.
The above method has overcome the drawback with plastic film, save time and laborsaving, both ventilation, printing opacity, preserve moisture and to make seedbed and nonwoven form the cuboid space temperature steady; Unlikely not high not low; Solved the certain illumination of seedling growth needs preferably, need the higher humidity of air when just transplanting, and unlikely soil humidity has been excessive; Be very beneficial for the transplanting survival of tissue cultivating seedling, adopt the method draft, woody training tissue culture seedling survival rate all can reach more than 95%.
Embodiment:
Be that example is described further the present invention with the transplanting in June of photinia glabra tissue cultivating seedling below:
1. prepare to transplant tissue cultivating seedling, treat that the photinia glabra seedling of taking root grows to the 2.5-3cm height, the white root of individual plant 3-4 bar during the long 1cm of root, is the transplanting optimum period.
2. build a nonwoven cuboid space of breathing freely, ventilate, preserving moisture by above-mentioned steps one described method.
3. select turfy soil, perlite, vermiculite mixed with 1:1:1; The mass concentration of using 50% carbendazol wettable powder to process is that millesimal carbendazim solution drenches; Do not drip, put down the breastheight degree to hold the agglomerating webs of matrix in hand and fall down the degree of being that to scatter; Pack in the 56 holes dishes, according to being the plane above the dish of cave, and scrape off unnecessary mixed-matrix.
4. clean shoot root portion agar, and divide large, medium and small three grades by the height of photinia glabra seedling.
5. transplant the photinia glabra tissue cultivating seedling.In the booth that shelters from heat or light, to plant the seedling of classification respectively in the dish of cave, the millesimal 50% carbendazol wettable powder solution of the mass percent of drenching is immediately made normal root water.Move in the above-mentioned 2. described seedbed of breathing freely, ventilate, preserving moisture and set, and use a nonwoven that covers a sprayer to squirt with the degree of being that do not drip.
6. the management after transplanting.
Often keep moistening on the nonwoven; Do i.e. water spray; Make that humidity remains on 95-100% in the cuboid space that seedling dish and nonwoven form, every 5-7 days, raise nonwoven and spray the medicine diseases prevention once with the millesimal carbendazim solution of 50% carbendazol wettable powder mass concentration; Logotype 3 times, disease-free basically generation.See that it is that new root forms that photinia glabra transplanted seedling length has 2-3 sheet young leaves, here do not wither with blade is standard, strengthens illumination gradually; Nursery stock is sturdy gradually, and resistance strengthens, and is indicating that the tissue cultivating seedling transplanting has adapted to external environment; The refining seedling is promptly accomplished, and the refining shoot survival percent reaches 96.5%.
Claims (3)
1. a tissue cultivating seedling is transplanted the technical method that covers nonwoven, it is characterized in that said method comprises following processing step:
Build in step 1, seedbed
On the seedbed, build a framework, cover at framework external application nonwoven then, make it to form the space of a seedbed and nonwoven formation;
Step 2, matrix are selected
Step 3, tissue cultivating seedling are cleaned
Clean tissue cultivating seedling root agar;
Step 4, tissue cultivating seedling are transplanted
In the booth that shelters from heat or light, contain the tissue cultivating seedling immigration in the seedling dish of matrix, spray liquid medicine, put again in the seedbed of the described nonwoven covering of step 1;
Management after step 5, the transplanting
At first, keep the moistening of nonwoven;
Secondly, every 5-7 days, raise nonwoven and spray diseases prevention once with liquid medicine.
2. a kind of tissue cultivating seedling according to claim 1 is transplanted the technical method that covers nonwoven; It is characterized in that matrix selects turfy soil, perlite and the vermiculite mixed with 1:1:1 or 2:2:1, adding the mass concentration that 50% carbendazol wettable powder processes then is that millesimal carbendazim solution drenches.
3. a kind of tissue cultivating seedling according to claim 1 and 2 is transplanted the technical method that covers nonwoven, it is characterized in that liquid medicine is that the mass concentration that 50% carbendazol wettable powder is processed is millesimal carbendazim solution.
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| Application Number | Priority Date | Filing Date | Title |
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| CN2012102352491A CN102771277A (en) | 2012-07-09 | 2012-07-09 | Technical method for nonwoven fabric covering during tissue culture seedling transplanting |
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| CN2012102352491A CN102771277A (en) | 2012-07-09 | 2012-07-09 | Technical method for nonwoven fabric covering during tissue culture seedling transplanting |
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| CN102771277A true CN102771277A (en) | 2012-11-14 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105393891A (en) * | 2015-11-26 | 2016-03-16 | 宁夏林业研究所股份有限公司 | Grape tissue culture seedling transplanting method for drought region |
| CN106034654A (en) * | 2016-06-03 | 2016-10-26 | 扬州大学 | Method for culturing cotton seedling in substrates through double layers of adhesive-bonded fabric |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1799338A (en) * | 2005-12-16 | 2006-07-12 | 江苏阳光生态农林开发股份有限公司 | Tissue-culturing and seedling-growing method for rosa canina |
-
2012
- 2012-07-09 CN CN2012102352491A patent/CN102771277A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1799338A (en) * | 2005-12-16 | 2006-07-12 | 江苏阳光生态农林开发股份有限公司 | Tissue-culturing and seedling-growing method for rosa canina |
Non-Patent Citations (2)
| Title |
|---|
| 姚桂峰: "水稻无纺布覆盖育苗技术", 《黑龙江科技信息》, no. 4, 28 February 2009 (2009-02-28), pages 239 - 238 * |
| 无: "有菌条件下的创新植物组培技术操作规范", 《HTTP://BLOG.SINA.COM.CN/S/BLOG_4AE51B5BO10009PQ.HTML》, 1 May 2007 (2007-05-01), pages 1 - 2 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105393891A (en) * | 2015-11-26 | 2016-03-16 | 宁夏林业研究所股份有限公司 | Grape tissue culture seedling transplanting method for drought region |
| CN105393891B (en) * | 2015-11-26 | 2018-05-25 | 宁夏林业研究所股份有限公司 | A kind of Tissue culture the seedling of grape method for transplanting of suitable arid area |
| CN106034654A (en) * | 2016-06-03 | 2016-10-26 | 扬州大学 | Method for culturing cotton seedling in substrates through double layers of adhesive-bonded fabric |
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Application publication date: 20121114 |