CN105393891B - A kind of Tissue culture the seedling of grape method for transplanting of suitable arid area - Google Patents
A kind of Tissue culture the seedling of grape method for transplanting of suitable arid area Download PDFInfo
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- CN105393891B CN105393891B CN201510833942.2A CN201510833942A CN105393891B CN 105393891 B CN105393891 B CN 105393891B CN 201510833942 A CN201510833942 A CN 201510833942A CN 105393891 B CN105393891 B CN 105393891B
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- seedling
- transplanting
- grape
- tissue culture
- gauze
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/02—Cultivation of hops or vines
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
- A01G13/02—Protective coverings for plants; Coverings for the ground; Devices for laying-out or removing coverings
- A01G13/0243—Protective shelters for young plants, e.g. tubular sleeves
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G9/00—Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
- A01G9/02—Receptacles, e.g. flower-pots or boxes; Glasses for cultivating flowers
- A01G9/029—Receptacles for seedlings
- A01G9/0299—Handling or transporting of soil blocks or seedlings
Abstract
The present invention relates to a kind of Tissue culture the seedling of grape method for transplanting of suitable arid area.Its main feature is that include the following steps：(1) hardening；(2) seedling and transplanting are washed；(3) moisturizing is covered；(4) final management and maintenance.The method of the present invention is a kind of simple and easy method of suitable northwest arid area grape detoxication and tissue culture transplantation of seedlings, and its advantage is：1. the method for the root using the quickly dipped grape tissue cultural seedlings of free to be transplanted of the root-growing agent and fungicide of compounding can promote the grape detoxication and tissue culture seedling rooting of transplanting and effectively prevent the Tissue culture the seedling of grape root of transplanting, especially rootstock is rotted；2. it is covered to ensure during Tissue culture the seedling of grape transplanting to the demand of air humidity by using the cotton gauze of bilayer moistening, both solved the problems, such as that bringing soil humidity by adjusting air humidity of spraying can accordingly increase, the technology requires facilities and equipment relatively low simultaneously, easy to operate.
Description
Technical field
The present invention relates to a kind of Tissue culture the seedling of grape method for transplanting of suitable arid area.
Background technology
Tissue culture the seedling of grape transplantation technique Tissue culture the seedling of grape factorial praluction in play the role of it is vital, be determine Portugal
One of key technology of success or failure is factory produced in grape tissue-cultured seedling.Research on Tissue culture the seedling of grape transplanting has a large amount of reports, and
The transplanting survival rate of Tissue culture the seedling of grape can reach more than 90%, but be only limitted to small-scale result of the test, for 10,000 plants of single batch
More than Tissue culture the seedling of grape transplant survival situation, has no and has been reported that.During Tissue culture the seedling of grape transplanting, the tissue-cultured seedling pair after transplanting
The humiture of air and the humiture of matrix are more demanding, especially in northwest arid area, are transplanted to newly from Tissue culture the seedling of grape
How the stage that root long goes out coordinates to resolve the stage Tissue culture the seedling of grape and requires high and soil humidity requirement moderate air humidity
The problem of, it is the key factor for improving Tissue culture the seedling of grape transplanting life rate.
Currently, solve the problems, such as that this is common there are two types of method：1. Tissue culture the seedling of grape intermediate house installation Temperature and Humidity Control system
System adjusts the aerial temperature and humidity in greenhouse by timing spraying, but the time due to spraying and spray amount are with required air
Humidity is standard, and it is excessive to be easy to cause spray amount so that the humidity of matrix is excessive, easy so as to cause the Tissue culture the seedling of grape of transplanting
Rotten, transplanting survival rate is reduced, meanwhile, this measure is more demanding to mating facilities and equipment；2. after Tissue culture the seedling of grape transplanting
It takes Small plastic shed and carries out moisturizing, this method not only can guarantee the air humidity needed for Tissue culture the seedling of grape transplanting in northwest arid area, but also
It can reduce and pour water to ensure that transplanting medium humidity is moderate, promote Tissue culture the seedling of grape transplanting survival rate, but this method is in northwest drought
Area can only apply in spring, for the summer and autumn of high temperature, since temperature is higher in Small plastic shed, can not carry out Tissue culture the seedling of grape
Transplanting.
The content of the invention
The object of the present invention is to provide a kind of Tissue culture the seedling of grape method for transplanting of suitable arid area, this method sets facility
It is standby to require relatively low, easy to operate, Tissue culture the seedling of grape transplanting survival rate height, and can ensure the uniformity of transplanting condition, it can
For the factorial praluction of Tissue culture the seedling of grape.
A kind of Tissue culture the seedling of grape method for transplanting of suitable arid area, is particular in that, includes the following steps：
(1) hardening：
By 5~8cm of plant height, the Tissue culture the seedling of grape of 2~3 roots moves to intermediate house from culturing room and carries out hardening, hardening phase
Between, temperature is controlled at 15~32 DEG C, and humidity relation control is 60%~70%；3~5d of hardening is first covered, then is unscrewed completely but not
Bottle cap hardening 1d is opened, then opens bottle cap, aqueous gauze is covered in bottleneck, until Tissue culture the seedling of grape leaf color is by light green color
Become green or bottle green；
(2) seedling and transplanting are washed：
The Tissue culture the seedling of grape for completing hardening from blake bottle is taken out, with clear water by root wash clean, root is trimmed to 3
After~4cm, it is in active ingredient and ultimate density：0.2mg/L IBA, 40~50 μ L/L hymexazols and the more bacterium of 0.4~0.6mg/L
It is quickly dipped in the mixing liquid of spirit, it is then transplanted in hole tray, transplanting medium ingredient is turf by volume:Vermiculite:Pearl
Rock=1:1:2, while the decay sheep excrement of 5% volume of the sum of turf, vermiculite, perlite is added, wherein transplanting medium is using
It is preceding uniformly to squirt disinfection with 50% carbendazim, 800~1000 times of liquid；
(3) moisturizing is covered：
With the Tissue culture the seedling of grape after aqueous cotton yarn covering transplanting, until new root is grown, gauze is opened；
(4) final management and maintenance：
The tissue-cultured seedling of transplanting is managed and protected after opening gauze：Specifically 50% carbendazim 800 is sprayed for 1 cycle per 10d
Times 1000 times of liquid of liquid or 80% Mancozeb, the two are used alternatingly so as to pre- disease prevention；0.1% is sprayed for 1 cycle per 10d~
0.2% potassium dihydrogen phosphate carries out foliage spray；By soil humidity control 40%~70%, cultivating 2~3 months can go out
Seedling.
Aqueous gauze is covered in step (1) in bottleneck to specifically refer to, the bilayer for being 30~70% in bottleneck covering water content
Cotton gauze, the density of gauze is (23~28) branch × (18~24) branch.
It is specifically referred in step (3) with the Tissue culture the seedling of grape after aqueous cotton yarn covering transplanting, is (23~28) with density
Branch × (18~24) branch, and the Tissue culture the seedling of grape after the double-deck cotton gauze covering transplanting that water content is 30~70%, daily more
Change gauze 4~6 times, the gauze replaced every time is required to 50% carbendazim, 1500~2000 times of liquid rinses, and the cover time is
10~15d.
The method of the present invention is a kind of simple and easy method of suitable northwest arid area grape detoxication and tissue culture transplantation of seedlings, beneficial to effect
Fruit is：1. the method for the root using the quickly dipped grape tissue cultural seedlings of free to be transplanted of the root-growing agent and fungicide of compounding, can promote
Into the grape detoxication and tissue culture seedling rooting of transplanting, and it can effectively prevent the Tissue culture the seedling of grape root of transplanting, especially rootstock is rotted；②
It covers to ensure both to solve the demand of air humidity during Tissue culture the seedling of grape transplanting by using the cotton gauze of bilayer moistening
The problem of soil humidity can accordingly increase brought by adjusting air humidity of spraying, and is solved through Small plastic shed moisturizing
Come band due in Small plastic shed temperature it is excessively high, can not be transplant summer and autumn the problem of, while the technology is to facilities and equipment requirement
It is relatively low, it is easy to operate；3. the grape tissue cultural seedlings of free transplanting survival rate transplanted using the present invention is high, and the uniformity of transplant survival
Good, the grape tissue cultural seedlings of free transplanting survival rate of 10,000 batches can reach more than 92.5%.
Specific embodiment
The method of the present invention includes following steps：
(1) hardening：
By 5~8cm of plant height, the Tissue culture the seedling of grape of 2~3 roots moves to intermediate house from culturing room and carries out hardening.The hardening phase
Between, temperature control in greenhouse is at 20~30 DEG C, and humidity relation control is 60%~70%；3~5d of hardening is first covered, then is unscrewed completely
But bottle cap hardening 1d is not opened, then opens bottle cap, the double-deck cotton gauze for being 30~70% in bottleneck covering humidity, until Portugal
Grape tissue-cultured seedling leaf color becomes green or bottle green by light green color；
(2) seedling and transplanting are washed：
The Tissue culture the seedling of grape for completing hardening from blake bottle is taken out, with clear water by root wash clean, root is trimmed to 3
After~4cm, it is in active ingredient and ultimate density：0.2mg/L IBA, 40~50 μ L/L hymexazols, the more bacterium of 0.4~0.6mg/L
It is quickly dipped in the mixing liquid of spirit, it is then transplanted in hole tray, transplanting medium ingredient is turf by volume:Vermiculite:Pearl
Rock=1:1:2, while the decay sheep excrement of 5% volume of the sum of turf, vermiculite, perlite is added, wherein transplanting medium is using
It is preceding uniformly to squirt disinfection with 50% carbendazim, 800~1000 times of liquid；
(3) moisturizing is covered：
× (18~24) branch is propped up for (23~28) with density, and after the double-deck Quality Pure Cotton Yarn Production covering transplanting that humidity is 30~70%
Tissue culture the seedling of grape, for ensure gauze humidity, according to the difference in season, gauze should be replaced daily 4~6 times, replaced every time
Gauze is required to 50% carbendazim, 1500~2000 times of liquid rinses, and the cover time is 10~15d, until new root is grown, is opened
Gauze.
(4) final management and maintenance：
The tissue-cultured seedling of transplanting is normally managed and protected after opening gauze：Per 10d 800 times of 50% carbendazim is sprayed for 1 cycle
Pre- disease prevention is used alternatingly in 1000 times of liquid of liquid and 80% Mancozeb.Be per 10d sprayed in 1 cycle quality system ratio be 0.1%~
0.2% potassium dihydrogen phosphate carries out foliage spray.40%~70%, cultivating 2~3 months can go out for soil humidity control
Seedling.
The present invention is during the transplanting of entire Tissue culture the seedling of grape, and temperature control is in 15~32 DEG C of models in tissue-cultured seedling intermediate house
Within enclosing, air humidity is controlled 60% or so.
Method for transplanting provided by the invention is for existing during the transplanting of current northwest arid area Tissue culture the seedling of grape
Problem, one is (active ingredient and ultimate density are using the root-growing agent and fungicide compounded：0.2mg/L IBA, 40~50 μ
L/L hymexazols, the mixing liquid of 0.4~0.6mg/L carbendazim) Tissue culture the seedling of grape is promoted to take root after transplanting and controls root rotten
It is rotten, second is that during the Tissue culture the seedling of grape transplanting respectively to uncap hardening and transplanting after to during raw new root (10 after transplanting~
15d) two stages select dual-layer density to be covered for the moistening cotton gauze of (23~28) branch × (18~24) branch to tissue-cultured seedling
Lid, the humidity of gauze is 30~70%, is covered by gauze, and the air humidity needed for control Tissue culture the seedling of grape transplanting subtracts simultaneously
The time sprayed less and spray amount, the effective humidity for controlling transplanting medium improve the transplanting of northwest arid area Tissue culture the seedling of grape
Survival rate.By the application of the method for the present invention, effectively ensure the uniformity of Tissue culture the seedling of grape transplant survival, realize 10,000 batches
Tissue culture the seedling of grape transplanting survival rate reach more than 92.5%, can meet Tissue culture the seedling of grape factorial praluction technology needs.It should
Method is easy to operate, of less demanding to facilities and equipment, easily operated.
Embodiment 1：
The spring transplanting (at the beginning of 4 months-by the end of May) of grape detoxification test tube plantlet.
(1) kind of transplanting：Cabernet Sauvignon, Vidal Blanc, the red earth；
(2) transplant step：
1. hardening：
By 5~8cm of plant height, the Tissue culture the seedling of grape of 2~3 roots moves to intermediate house from culturing room and carries out hardening.The hardening phase
Between, temperature control in greenhouse is at 15~32 DEG C, and humidity relation control is 60%~70%；3~5d of hardening is first covered, then is unscrewed completely
But bottle cap (i.e. bottle cap directly overlays bottleneck, and one, which carries both bottle caps, separates) hardening 1d is not opened, then opens bottle cap,
The double-deck cotton gauze that bottleneck covering humidity is 30~70%, the density of gauze are propped up for (23~28) branch × (18~24), hardening 3
~4d, until Tissue culture the seedling of grape leaf color becomes green or bottle green by light green color.
2. wash seedling and transplanting：
The grape tissue cultural seedlings of free for completing hardening with tweezers carefully from blake bottle is taken out, is carried root with clear water
Agar wash clean, root is trimmed to 3-4cm, is in active ingredient and ultimate density：0.2mg/L IBA, 40 μ L/L dislike mould
Quickly dipped and then be transplanted in 50 hole trays in the mixing liquid of spirit and 0.4mg/L carbendazim, transplanting medium ingredient is turf:Leech
Stone:Perlite=1:1:2 (V/V/V), while the decay sheep excrement of 5% volume of the sum of turf, vermiculite, perlite is added, wherein
Transplanting medium uniformly squirts disinfection with 50% carbendazim, 800~1000 times of liquid before use.
3. cover moisturizing：
× (18~24) branch is propped up for (23~28) with density, and after the double-deck Quality Pure Cotton Yarn Production covering transplanting that humidity is 30~70%
Grape tissue cultural seedlings of free, to ensure the humidity of gauze, replace gauze 4 times daily, the gauze replaced every time is required to 50%
1500~2000 times of liquid rinses of carbendazim, cover time are 12~15d, until new root is grown, open gauze.
4. final management and maintenance：
The tissue-cultured seedling of transplanting is normally managed and protected after opening gauze：Per 10d 800 times of 50% carbendazim is sprayed for 1 cycle
1000 times of liquid of liquid or 80% Mancozeb, two kinds of pre- disease preventions of liquid alternated use.Per 10d mass volume ratio is sprayed for 1 cycle
Foliage spray is carried out for 0.1%~0.2% potassium dihydrogen phosphate.Soil humidity control cultivates 2~3 40%~70%
The moon can emerge.
Embodiment 2：
The summer and autumn of grape detoxification test tube plantlet is transplanted (at the beginning of 6 months at the beginning of -10 months).
(1) kind of transplanting：Cabernet Sauvignon, Vidal Blanc, the red earth；
(2) transplant step：
1. hardening：
The step of with embodiment 1, is 1. identical；
2. wash seedling and transplanting：
The step of with embodiment 1, is 2. identical, but for the formula of the quickly dipped mixing liquid in root：0.2mg/L IBA, 50 μ
L/L hymexazols, it is quickly dipped in the mixing liquid of 0.6mg/L carbendazim.
3. cover moisturizing：
The step of with embodiment 1, is 3. identical, but replaces the number of gauze every time as 6 times, and the cover time is 10~12d；
4. final management and maintenance：
The step of with embodiment 1, is 4. identical.
Claims (1)
1. a kind of Tissue culture the seedling of grape method for transplanting of suitable arid area, which is characterized in that include the following steps：
（1）Hardening：
By 5~8cm of plant height, the Tissue culture the seedling of grape of 2~3 roots moves to intermediate house from culturing room and carries out hardening, during hardening, temperature
Degree control is at 15~32 DEG C, and humidity relation control is 60%~70%；3~5d of hardening is first covered, then unscrews completely but does not open bottle
Lid hardening 1d, then opens bottle cap, and aqueous gauze is covered in bottleneck, until Tissue culture the seedling of grape leaf color becomes green by light green color
Color or bottle green；
（2）Wash seedling and transplanting：
The Tissue culture the seedling of grape for completing hardening is taken out from blake bottle, with clear water by root wash clean, root is trimmed to 3~
After 4cm, it is in active ingredient and ultimate density：0.2mg/L IBA, 40~50 μ L/L hymexazols and 0.4~0.6 mg/L are more
It is quickly dipped in the mixing liquid of bacterium spirit, it is then transplanted in hole tray, transplanting medium ingredient is turf by volume:Vermiculite:It is precious
Zhu Yan=1:1:2, while the decay sheep excrement of 5% volume of the sum of turf, vermiculite, perlite is added, wherein transplanting medium is using
It is preceding uniformly to squirt disinfection with 50% carbendazim, 800~1000 times of liquid；
（3）Cover moisturizing：
With the Tissue culture the seedling of grape after aqueous cotton yarn covering transplanting, until new root is grown, gauze is opened；
（4）Final management and maintenance：
The tissue-cultured seedling of transplanting is managed and protected after opening gauze：Specifically per 10d for 1 cycle spray 50% carbendazim, 800 times of liquid or
80% 1000 times of Mancozeb liquid, the two are used alternatingly so as to pre- disease prevention；Per 10d 0.1%~0.2% di(2-ethylhexyl)phosphate is sprayed for 1 cycle
Hydrogen potassium solution carries out foliage spray；By soil humidity control 40%~70%, cultivating 2~3 months can emerge；
Step（1）In cover aqueous gauze in bottleneck and specifically refer to, the double-deck Quality Pure Cotton Yarn Production for being 30~70% in bottleneck covering water content
Cloth, the density of gauze are（23~28）Branch ×（18~24）Branch；
Step（3）The middle Tissue culture the seedling of grape with after aqueous cotton yarn covering transplanting specifically refers to, and is with density（23~28）Branch ×
（18~24）Branch, and the Tissue culture the seedling of grape after the double-deck cotton gauze covering transplanting that water content is 30~70%, replace gauze daily
4~6 times, the gauze replaced every time is required to 50% carbendazim, 1500~2000 times of liquid rinses, and the cover time is 10~15d.
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| CN106212189A (en) * | 2016-08-29 | 2016-12-14 | 北京神舟绿鹏农业科技有限公司 | A kind of method that Arundo donax is planted at saline and alkaline desert Land Size |
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| CN101564012A (en) * | 2009-05-18 | 2009-10-28 | 杭州蓝天园林建设集团有限公司余杭分公司 | Seedling exercising replanting technique for test-tube plantlet of raisin grape |
| CN102771277A (en) * | 2012-07-09 | 2012-11-14 | 江西省喜果绿化有限公司 | Technical method for nonwoven fabric covering during tissue culture seedling transplanting |
| CN104206242A (en) * | 2014-09-16 | 2014-12-17 | 宁夏农垦西夏王实业有限公司葡萄苗木分公司 | Annual factory production process for grape virus-free tube seedlings |
| CN104938339A (en) * | 2015-06-29 | 2015-09-30 | 大新县科学技术情报研究所 | Rapid propagation method for tissue culture of grape |
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2015
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101564012A (en) * | 2009-05-18 | 2009-10-28 | 杭州蓝天园林建设集团有限公司余杭分公司 | Seedling exercising replanting technique for test-tube plantlet of raisin grape |
| CN102771277A (en) * | 2012-07-09 | 2012-11-14 | 江西省喜果绿化有限公司 | Technical method for nonwoven fabric covering during tissue culture seedling transplanting |
| CN104206242A (en) * | 2014-09-16 | 2014-12-17 | 宁夏农垦西夏王实业有限公司葡萄苗木分公司 | Annual factory production process for grape virus-free tube seedlings |
| CN104938339A (en) * | 2015-06-29 | 2015-09-30 | 大新县科学技术情报研究所 | Rapid propagation method for tissue culture of grape |
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