CN107646479B - A kind of propagation method of five happinesses persimmon seedling - Google Patents
A kind of propagation method of five happinesses persimmon seedling Download PDFInfo
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- CN107646479B CN107646479B CN201711011511.3A CN201711011511A CN107646479B CN 107646479 B CN107646479 B CN 107646479B CN 201711011511 A CN201711011511 A CN 201711011511A CN 107646479 B CN107646479 B CN 107646479B
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- 235000011511 Diospyros Nutrition 0.000 title claims abstract description 53
- 244000236655 Diospyros kaki Species 0.000 title claims abstract description 53
- 238000000034 method Methods 0.000 title claims abstract description 24
- 235000013399 edible fruits Nutrition 0.000 claims abstract description 74
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 22
- 235000016709 nutrition Nutrition 0.000 claims abstract description 9
- 230000035764 nutrition Effects 0.000 claims abstract description 9
- 238000004140 cleaning Methods 0.000 claims abstract description 6
- 230000035784 germination Effects 0.000 claims abstract description 6
- 241000196324 Embryophyta Species 0.000 claims abstract description 4
- 230000001954 sterilising effect Effects 0.000 claims abstract description 3
- 239000002689 soil Substances 0.000 claims description 17
- 239000007921 spray Substances 0.000 claims description 14
- 239000004576 sand Substances 0.000 claims description 11
- 239000006041 probiotic Substances 0.000 claims description 7
- 235000018291 probiotics Nutrition 0.000 claims description 7
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 6
- 229930006000 Sucrose Natural products 0.000 claims description 6
- 239000005720 sucrose Substances 0.000 claims description 6
- -1 pulp Substances 0.000 claims description 5
- 238000002054 transplantation Methods 0.000 claims description 5
- 238000002791 soaking Methods 0.000 claims description 2
- 238000004659 sterilization and disinfection Methods 0.000 claims description 2
- 239000011521 glass Substances 0.000 claims 1
- 230000004083 survival effect Effects 0.000 abstract description 7
- 241000894007 species Species 0.000 abstract description 3
- 238000004519 manufacturing process Methods 0.000 abstract description 2
- TWFZGCMQGLPBSX-UHFFFAOYSA-N carbendazim Chemical compound C1=CC=C2NC(NC(=O)OC)=NC2=C1 TWFZGCMQGLPBSX-UHFFFAOYSA-N 0.000 description 3
- 238000007654 immersion Methods 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 239000002574 poison Substances 0.000 description 3
- 231100000614 poison Toxicity 0.000 description 3
- 239000004563 wettable powder Substances 0.000 description 3
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- 239000011435 rock Substances 0.000 description 2
- 229910000831 Steel Inorganic materials 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008033 biological extinction Effects 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000035040 seed growth Effects 0.000 description 1
- 239000010959 steel Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C21/00—Methods of fertilising, sowing or planting
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Soil Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Botany (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The present invention relates to fruit tree propagation methods, disclose a kind of propagation method of five happinesses persimmon seedling.The present invention after the pulp cleaning and sterilizing by five happinesses persimmon by being made fruit ferment；Five happinesses persimmon seed is put into fruit ferment and is impregnated, germination is promoted to take root；Fruit ferment is equipped with to quantitative clear water after transplanting, periodically sprout pour applying, primary nutrition is provided by seedling of fruit ferment, seedling is finally made to form the plant of normal stalwartness；And then rescue this rareness species that will be become extinct.The propagation method of fruit ferment nursery disclosed by the invention, it is more than 80% to make the survival rate of five happinesses persimmon seed seedling-raising, realizes the five happinesses persimmon sapling multiplication breakthrough of zero, is of great significance to the protection of species, research, production, application and popularization.
Description
Technical field
The present invention relates to fruit tree propagation methods, and in particular to a kind of to breed rare plant five happinesses persimmon kind in imminent danger using fruit ferment
The method of seedling.
Background technology
Five happinesses persimmon, scientific name Beijing persimmon, source area Beijing, fruit surface has protrusion at five, thus is named five happinesses persimmon.Currently,
Five happinesses persimmon in China's only deposits two, and one is grown in Beijing, is planted within maximum monastery Yonghe Palace Temple, Beijing, but this
It will not bloom；Another is taken to south in teacher Mr. Hui Shiqi of Kangxu 60 years by Qianrong from Beijing, is planted general in Guangdong
The Ningnan temples Yan Gu, nearly 300 years so far, during mass movement to make iron and steel in 1958, southern rock Gu temple five happinesses persimmon body split one by axe
Half, it nearly destroys, trunk still has axe mark so far.With the aging of five happinesses persimmon ancient tree, which has the danger of extinction.
In the sixties in last century, the expert of academy of agricultural sciences of Guangdong Province, which was once stationed at southern rock Gu temple, attempts to breed five happinesses persimmon, however
Various methods are used up, the final result of experiment is：Five happinesses persimmon seed is difficult to breed, cuttage will not take root, graft and can not survive,
The scientific research activity finally ends in failure.Agriculture section personnel at different levels breed five using modes such as aerial nursery, cell tissue cultures again later
Good fortune persimmon seedling is all counted out.Therefore, selection appropriate method to the seedling of five happinesses persimmon breed extremely urgent, most important.
Invention content
It is an object of the invention to overcome the problems, such as that the above-mentioned persimmon seedling of five happinesses in the prior art is difficult to breed, one kind five is provided
The propagation method of good fortune persimmon seedling.
To achieve the above object, technical scheme is as follows：
A kind of propagation method of five happinesses persimmon seedling, the propagation steps are as follows successively：
S1, being placed on five happinesses persimmon cleaning and sterilizing in temperature-controlling chamber, when the pericarp of fruit turns yellow, by seed from fruit
It is taken out in reality, after being embedded in wet sand for a period of time, further takes out disinfection；
S2, after fruit ferment is made in the pulp of five happinesses persimmon, pomace is filtered out from fruit ferment, system is mixed with fertile soil
At Nutrition Soil；
S3, by after being impregnated in step S1 treated fruit ferment that seed, which puts into, filters off pomace, pull out, be sprinkling upon Nutrition Soil
On, and one layer of wet sand is capped on seed；
S4, the fruit ferment for impregnating seed in step S3 is sealed in glassware, when seed to be poured, fruit ferment is fallen
Go out to mix with water and spray；
S5, when seed start germination when, adjust fruit ferment and water mixed proportion, periodically spray；When five happinesses persimmon seedling is given birth to
Root and it is long to five leaves when, will kind transplantation of seedlings to flowerpot transplant or garden between be colonized.
When the leaf number of seedling is grown to 5 pieces, the seedling root system is more flourishing, has reached transplanting condition.
Preferably, the five happinesses persimmon of the step S1 be the five happinesses persimmon mature period win completely filled fruit, development it is good
Good fruit.
Preferably, the temperature control room temperature of the step S1 is 20 DEG C -30 DEG C.
Preferably, the seed of the step S1 is embedded in the time in wet sand as 3d-5d.
Further, the fruit ferment of the step S2 is made by the steps：Weigh a certain amount of sucrose, fruit
Meat, water are put into glassware, are sealed after probiotics is added, then placed three to five days in temperature-controlling chamber, that is, fruit ferment is made.
Further, the sucrose, pulp, water ratio be 1:1.5:8, the weight percent of the probiotics
0.1%-2%, the temperature control room temperature are 25 DEG C.
Further, pomace and mixed proportion humous are 1 in the Nutrition Soil of the step S2:12.
Preferably, the seed soaking time of the step S3 is for 24 hours.
Further, the mixed proportion of fruit ferment and water is 1 in the step S4:20, it is daily one to spray number
It is secondary.
Further, the mixed proportion of fruit ferment and water is 1 in the step S5:10, it sprays daily twice.
Beneficial effects of the present invention are：
（1）Present invention employs a kind of completely new seedling-raising techniques：Fruit ferment seedling-raising technique, and success nursery, it is numerous to be difficult to
The seedling grown provides new seedling raising manners.
（2）Propagation method provided by the invention realizes the five happinesses persimmon sapling multiplication breakthrough of zero, and survival rate is more than 80%,
It is of great significance to the species conservation of this rare plant in imminent danger of five happinesses persimmon, research, production, application and popularization.
Specific implementation mode
The present invention is described in detail with reference to specific embodiment.
Embodiment 1
A kind of propagation method of five happinesses persimmon seedling, the propagation steps are as follows successively：
S1, in the five happinesses persimmon mature period（Or so mid-August）, completely filled fruit, well-developed fruit are won, cleaning disappears
Poison is placed in 20 DEG C of temperature-controlling chamber；When the pericarp of fruit is by green flavescence, seed is taken out from fruit, is embedded in 3 in wet sand
It after it, further takes out to be soaked in 50% carbendazol wettable powder, 300 times of liquid and sterilizes, pulled out after ten minutes；
S2, the pulp of five happinesses persimmon is collected, by 1:1.5:8 ratio weighs sucrose, pulp, water, is put into vierics
In ware, is sealed after the probiotics that weight percent is 0.1% is added, then placed three days in 25 DEG C of temperature-controlling chamber, fruit ferment is made
Element；Pomace is filtered out from fruit ferment finally, pomace and fertile soil are pressed 1:12 ratio is mixed and made into Nutrition Soil；
S3, by through step S1, treated that seed puts into filters off in the fruit ferment of pomace, immersion is pulled out afterwards for 24 hours, is sprinkling upon battalion
It supports on soil, and is capped one layer of wet sand on seed；
S4, the fruit ferment for impregnating seed in step S3 is continued to be sealed in glassware, when seed to be poured, by fruit ferment
Element is poured out with water with 1:20 ratio mixing sprays, once a day；
S5, when seed start germination when（Probably after two weeks）, the mixed proportion of fruit ferment and water is adjusted to 1:10, daily
It sprays twice；When five happinesses persimmon kind seedling rooting and it is long to five leaves when, will kind transplantation of seedlings to flowerpot transplant or garden between be colonized.
Embodiment 2
A kind of propagation method of five happinesses persimmon seedling, the propagation steps are as follows successively：
S1, in the five happinesses persimmon mature period（Or so mid-August）, completely filled fruit, well-developed fruit are won, cleaning disappears
Poison is placed in 25 DEG C of temperature-controlling chamber；When the pericarp of fruit is by green flavescence, seed is taken out from fruit, is embedded in 4 in wet sand
It after it, further takes out to be soaked in 50% carbendazol wettable powder, 200 times of liquid and sterilizes, pulled out after ten minutes；
S2, the pulp of five happinesses persimmon is collected, by 1:1.5:8 ratio weighs sucrose, pulp, water, is put into vierics
In ware, is sealed after the probiotics that weight percent is 1% is added, then placed four days in 25 DEG C of temperature-controlling chamber, fruit ferment is made；
Pomace is filtered out from fruit ferment finally, pomace and fertile soil are pressed 1:12 ratio is mixed and made into Nutrition Soil；
S3, by through step S1, treated that seed puts into filters off in the fruit ferment of pomace, immersion is pulled out afterwards for 24 hours, is sprinkling upon battalion
It supports on soil, and is capped one layer of wet sand on seed；
S4, the fruit ferment for impregnating seed in step S3 is continued to be sealed in glassware, when seed to be poured, by fruit ferment
Element is poured out with water with 1:20 ratio mixing sprays, once a day；
S5, when seed start germination when（Probably after two weeks）, the mixed proportion of fruit ferment and water is adjusted to 1:10, daily
It sprays twice；When five happinesses persimmon kind seedling rooting and it is long to five leaves when, will kind transplantation of seedlings to flowerpot transplant or garden between be colonized.
Embodiment 3
A kind of propagation method of five happinesses persimmon seedling, the propagation steps are as follows successively：
S1, in the five happinesses persimmon mature period（Or so mid-August）, completely filled fruit, well-developed fruit are won, cleaning disappears
Poison is placed in 30 DEG C of temperature-controlling chamber；When the pericarp of fruit is by green flavescence, seed is taken out from fruit, is embedded in 5 in wet sand
It after it, further takes out to be soaked in 50% carbendazol wettable powder, 150 times of liquid and sterilizes, pulled out after ten minutes；
S2, the pulp of five happinesses persimmon is collected, by 1:1.5:8 ratio weighs sucrose, pulp, water, is put into vierics
In ware, is sealed after the probiotics that weight percent is 2% is added, then placed five days in 25 DEG C of temperature-controlling chamber, fruit ferment is made；
Pomace is filtered out from fruit ferment finally, pomace and fertile soil are pressed 1:12 ratio is mixed and made into Nutrition Soil；
S3, by through step S1, treated that seed puts into filters off in the fruit ferment of pomace, immersion is pulled out afterwards for 24 hours, is sprinkling upon battalion
It supports on soil, and is capped one layer of wet sand on seed；
S4, the fruit ferment for impregnating seed in step S3 is continued to be sealed in glassware, when seed to be poured, by fruit ferment
Element is poured out with water with 1:20 ratio mixing sprays, once a day；
S5, when seed start germination when（Probably after two weeks）, the mixed proportion of fruit ferment and water is adjusted to 1:10, daily
It sprays twice；When five happinesses persimmon kind seedling rooting and it is long to five leaves when, will kind transplantation of seedlings to flowerpot transplant or garden between be colonized.
Embodiment 4
Following table is the survival rate comparison sheet of embodiment 1-3 and control group, and control group is the implementation that fruit ferment is substituted for water
Example 1
| Cultivate batch | Embodiment 1 | Embodiment 2 | Embodiment 3 | Control group |
| Seed number | 100 | 100 | 100 | 100 |
| Survive number | 86 | 83 | 82 | 0 |
| Survival rate | 86% | 83% | 82% | 0 |
As can be seen from the above table, fruit ferment method for culturing seedlings of the invention is up to 80% or more by the survival rate of five happinesses persimmon seedling,
And the control group survival rate for being not added with fruit ferment is zero.
The fruit ferment of the present invention is made using five happinesses persimmon pulp by raw material, provides the nutrition needed for seed growth, is
Rooting for five happinesses persimmon seed provides good condition.The fruit ferment propagation method of the present invention, realizes five happinesses persimmon seedling
The breakthrough of zero is bred, and survival rate is more than 80%, reproductive-cost is low.
Finally it should be noted that：Obviously, the above embodiment is merely an example for clearly illustrating the present invention, and simultaneously
The non-restriction to embodiment.For those of ordinary skill in the art, it can also do on the basis of the above description
Go out other various forms of variations or variation.There is no necessity and possibility to exhaust all the enbodiments.And thus drawn
The obvious changes or variations that Shen goes out are still in the protection scope of this invention.
Claims (5)
1. a kind of propagation method of five happinesses persimmon seedling, which is characterized in that the propagation steps are as follows successively：
S1, completely filled fruit, the well-developed fruit won of selection five happinesses persimmon mature period, by five happinesses persimmon cleaning and sterilizing it
It is placed in temperature-controlling chamber, temperature control room temperature is 20 DEG C -30 DEG C, when the pericarp of fruit turns yellow, seed is taken out from fruit, is buried
In wet sand after 3d-5d, disinfection is further taken out；
S2, after fruit ferment is made in the pulp of five happinesses persimmon, pomace is filtered out from fruit ferment, battalion is mixed and made into fertile soil
Support soil；
S3, by after being impregnated in step S1 treated fruit ferment that seed, which puts into, filters off pomace, pull out, be sprinkling upon on Nutrition Soil,
And one layer of wet sand is capped on seed；
S4, the fruit ferment that seed was impregnated in step S3 is sealed in glassware, when seed to be poured, by fruit ferment pour out with
Water mixing sprays；
S5, when seed start germination when, adjust fruit ferment and water mixed proportion, periodically spray；When five happinesses persimmon kind seedling rooting simultaneously
When length is to five leaves, kind transplantation of seedlings is colonized between flowerpot plant or garden；
Wherein, the fruit ferment of step S2 is made by the steps：It weighs a certain amount of sucrose, pulp, water and is put into glass
In vessel, is sealed after probiotics is added, placed three to five days in temperature-controlling chamber, that is, fruit ferment is made；The weight percent of probiotics
Compare 0.1%-2%.
2. a kind of propagation method of five happinesses persimmon seedling according to claim 1, which is characterized in that the battalion of the step S2
It is 1 to support pomace and mixed proportion humous in soil:12.
3. a kind of propagation method of five happinesses persimmon seedling according to claim 1, which is characterized in that the kind of the step S3
Sub- soaking time is for 24 hours.
4. a kind of propagation method of five happinesses persimmon seedling according to claim 1, which is characterized in that fruit in the step S4
The mixed proportion of ferment and water is 1:20, it is once a day to spray number.
5. a kind of propagation method of five happinesses persimmon seedling according to claim 1, which is characterized in that fruit in the step S5
The mixed proportion of ferment and water is 1:10, it sprays daily twice.
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| CN109089466B (en) * | 2018-09-05 | 2021-05-11 | 天津农学院 | Method for promoting celery seed germination |
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| KR101374501B1 (en) * | 2012-08-10 | 2014-03-14 | 김형덕 | Culture solution containing taurine and method for cultivating crop containing taurine using the same |
| CN105075634A (en) * | 2015-09-02 | 2015-11-25 | 黄冈师范学院 | Method for cultivating high-quality nursery stocks of sweet persimmon by enabling seeds of sweet persimmon to germinate fast and efficiently |
| CN105493993B (en) * | 2015-12-01 | 2018-07-20 | 厦门腾建绿化工程有限公司 | The fast breeding method of Queensland nut stock |
| CN105557421A (en) * | 2015-12-09 | 2016-05-11 | 安徽和合园林绿化工程有限公司 | Persimmon tree seedling cuttage cultivation method |
| CN106305291A (en) * | 2016-08-22 | 2017-01-11 | 金寨县万紫千红农业科技开发有限公司 | Wild gingko natural seedling healthy planting method |
| CN106386475A (en) * | 2016-09-28 | 2017-02-15 | 河南省长义农业科技有限公司 | Phytoenzyme mutagenesis grafting breeding method |
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| CN106718515A (en) * | 2016-11-26 | 2017-05-31 | 防城港市林木良种繁育中心苗圃 | A kind of method for accelerating the germination of cuttage banyan |
| CN106577006A (en) * | 2017-01-21 | 2017-04-26 | 山东省果树研究所 | Sweet persimmon container seedling raising matrix and method |
| CN107047617A (en) * | 2017-04-27 | 2017-08-18 | 东安县源丰农业科技发展有限公司 | Low temperature abscisic acid and its application method |
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