CN113678703B - Ginger detoxification and probiotics combination protection cultivation - Google Patents
Ginger detoxification and probiotics combination protection cultivation Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/25—Root crops, e.g. potatoes, yams, beet or wasabi
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/08—Immunising seed
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/34—Nitriles
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
- A01N47/28—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N<
- A01N47/34—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N< containing the groups, e.g. biuret; Thio analogues thereof; Urea-aldehyde condensation products
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
- A01N63/22—Bacillus
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Abstract
The invention discloses a novel ginger detoxification and probiotic combination protection carrier culture, which comprises the following steps: step (1): washing off soil on the surface of ginger seeds by using underground water or tap water, soaking the ginger seeds in a washing liquid, washing and cleaning the ginger seeds by using underground water, sending the ginger seeds into a detoxification room which is sterilized by a physical method by means of a combined method, and then fumigating the ginger seeds; step (II): the detoxification room is subjected to germination accelerating treatment, after 15-20 days of heat treatment, when few terminal buds start to germinate, ginger seeds are sprayed with gibberellin liquid to wet the surface, and a plurality of adventitious buds are generated on the surface of the ginger after one week of treatment. The novel ginger detoxification and probiotic combination protection carrier culture can effectively prevent ginger diseases, and utilizes probiotic protection culture, so that green pollution is realized, the yield increasing effect is obvious, the disease resistance and high temperature resistance provide a green culture method for food with homology of medicine and food, and a novel idea and novel approach for ginger industrialization development.
Description
Technical Field
The invention relates to the technical field of chemical industry, in particular to ginger detoxification and probiotic joint protection cultivation.
Background
In the process of the industrialized development of ginger, the production of ginger is restricted by high cost, the consumption of ginger seeds per mu is about 700-1000 jin, and ginger blocks are directly planted in a field, and some viruses in ginger seeds are difficult to remove, so that the ginger cannot be produced in high yield. In addition, ginger is planted, and the planting time is equal to the adverse natural factors such as drought and the like before and after Qingming festival. The ginger is difficult to sprout, the ginger blocks are rotten, the sprout is not regular, the ginger is difficult to produce at high yield, and the phenomena of high planting cost and less recovery are caused.
The ginger bud seedling technology is a biological microecological technology which combines tissue culture technology with traditional asexual propagation and biotechnology, integrates physical, chemical and biological technologies, combines tissue culture, heat treatment detoxification, chemical epidemic prevention and agricultural technology, and cultures beneficial biological bacteria. Ensuring the differentiation of the induced callus, the generation of adventitious buds, increasing the value, and preemptively implanting beneficial microorganisms, so that the microorganisms and plants are symbiotic and mutually promoted, the microorganisms promote the growth of the plants to improve the nutrition, the plants obtain nutrients and microelements which are difficult to obtain by the conventional technology, and a novel method for producing a product without pesticide residues is provided.
Disclosure of Invention
The invention aims to provide a ginger detoxification and probiotics combination protection cultivation method so as to solve the problems that the existing ginger is difficult to emerge, ginger blocks are rotten, the emergence is irregular, the ginger is difficult to produce in a high yield, the planting cost is high, and the recovery is less.
In order to achieve the above purpose, the present invention provides the following technical solutions: ginger detoxification and probiotic combination protection cultivation comprises the following steps:
step (1): washing off soil on the surface of ginger seeds by using underground water or tap water, soaking the ginger seeds in a washing liquid, washing and cleaning the ginger seeds by using underground water, and sending the ginger seeds into a detoxification room which is sterilized by a physical and chemical comprehensive method, and then fumigating the ginger seeds;
step (II): the germination accelerating treatment is carried out on the detoxification room, the detoxification room is kept at the relative humidity of 60%, the temperature is 40 ℃ in the daytime and 35 ℃ at night, after the heat treatment for 15-20 days, when few terminal buds begin to germinate, the surface of ginger seeds is sprayed with gibberellin liquid, and a plurality of adventitious buds are generated on the surface of the ginger seeds after one week of treatment;
step (III): taking adventitious buds as units, cutting a piece of tissue every two buds; soaking cut ginger seeds in 6-BA0.5mg/L+NAA0.1mg/L, placing in a detoxification room for 1 hour, soaking ginger seeds in the comprehensive bacterial liquid of bacillus subtilis and Japanese em bacteria, and then transferring into a greenhouse for cultivation;
step (IV): firstly, filling nutrition mud into which probiotics are implanted into a nutrition cup, then embedding ginger seeds into the nutrition cup to a depth of 1mm, then watering with bacterial liquid which is jointly cultured by 0.1% of ginger block extract and probiotics, enabling ginger seedlings to come out of soil after 15 days, and then culturing until three true leaves are grown in root systems, wherein the root systems are basically full of the nutrition cup;
in the third step, the temperature of the greenhouse is controlled to be 23-25 ℃ and the humidity is 70%;
step (five): separating old tissue, adding thiophanate-methyl into talcum powder, and then treating wounds by using sterilizing powder prepared from chlorothalonil;
in the step (five), the concentration of the thiophanate-methyl is 70 percent, and the concentration of the sterilizing powder prepared from the chlorothalonil is 75 percent;
step (six): the ginger seedlings are transplanted into a field for cultivation, 4600 plants are planted per mu, and the old tissues are subjected to secondary greenhouse cultivation; obtaining two batches of ginger seedlings altogether;
step (seven): and (3) comparing the two batches of ginger seedlings obtained in the step (six).
Preferably, the concentration of the washing liquid in the step (one) is 5%, and the soaking time of the ginger seeds in the washing liquid is 10 minutes.
Preferably, in the step (II), the concentration of gibberellin liquid is 50mg/L, the humidity of the detoxification room after spraying is adjusted to 75%, and the temperature is adjusted to 23 ℃ in the daytime and 20 ℃ at night.
Compared with the prior art, the invention has the beneficial effects that: the ginger is subjected to detoxification and probiotic combination protection cultivation;
1. the obtained ginger seedling has no young leaves, emerald green and relaxed leaves, strong stems and leaves, developed root systems, exuberant plants, disease resistance, high temperature resistance, strong overgrowth resistance, yield of over 5000 kg per mu, reduction of adverse factors of asexual propagation in traditional cultivation, provision of a more superior environment for the plants, increase of yield by 60%, effective reduction of cost, improvement of efficiency, utilization of biotechnology protection, maximum reduction of pesticide use and food safety;
2. the method has the advantages of effectively preventing and treating ginger diseases, protecting and cultivating by utilizing probiotics, realizing green pollution, having obvious yield increasing effect, providing a green cultivation method for food with homology of medicine and food by disease resistance and high temperature resistance, and providing a new way of thinking for the industrialized development of ginger.
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FIG. 1 shows a comparison of the novel method of asexual propagation and cultivation according to the invention.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Embodiment one:
ginger detoxification and probiotic combination protection cultivation, the production and application thereof are described in detail below:
step (1): washing off soil on the surface of ginger seeds by using underground water or tap water, soaking the ginger seeds in a washing liquid, washing the ginger seeds by using underground water, sending the ginger seeds into a detoxification room which is sterilized by a physical and chemical comprehensive method, and fumigating the ginger seeds, wherein the concentration of the washing liquid is 5%, and the soaking time of the ginger seeds in the washing liquid is 10 minutes;
step (II): the method comprises the steps of (1) carrying out germination accelerating treatment on a detoxification room, keeping the detoxification room at a relative humidity of 60%, and carrying out heat treatment at a temperature of 40 ℃ in daytime and 35 ℃ at night, wherein when a few terminal buds begin to germinate, spraying gibberellin liquid on the surface of ginger seeds, and carrying out treatment for one week, wherein a plurality of adventitious buds are generated on the surface of the ginger seeds, the concentration of the gibberellin liquid is 50mg/L, the humidity of the detoxification room is adjusted to 75% after the spraying, the temperature is adjusted to 23 ℃ in daytime and 20 ℃ at night;
step (III): a piece of tissue was cut every two shoots in units of adventitious shoots. Soaking cut ginger in 6-BA0.5mg/L+NAA0.1mg/L, placing in a detoxification room for 1 hour, soaking ginger seeds in bacillus and added bacteria liquid, and then transferring into a greenhouse for cultivation, wherein the bacillus immersed in the ginger seeds is bacillus subtilis (in a probiotic single bacteria combination for easy disease prevention and control of ginger), and the soaked bacteria liquid is a comprehensive bacteria liquid of Japanese em bacteria;
step (IV): firstly, filling nutrition mud into which probiotics are implanted into a nutrition cup, then, embedding ginger seeds into the nutrition cup to a depth of 1mm (the standard that ginger blocks cannot be seen), then, watering with bacterial liquid which is prepared by jointly culturing 0.1% of ginger block extract and probiotics, enabling the humidity to be 75%, after 15 days, enabling ginger seedlings to come out of soil, then, culturing three true leaves, enabling root systems to be basically full of the nutrition cup, and controlling the temperature of a greenhouse to be 23 ℃ and the humidity to be 70%.
Step (five): separating old tissue, adding thiofidaxl methyl into talcum powder, and then preparing sterilizing powder by combining chlorothalonil, wherein the concentration of the thiofidaxl methyl is 70%, and the concentration of the sterilizing powder prepared by the chlorothalonil is 75%;
step (six): ginger seedlings are transplanted into a field for cultivation, about 4600 plants are planted in each mu (667 square), and the old tissues are subjected to secondary greenhouse cultivation; obtaining two batches of ginger seedlings altogether;
step (seven): and (5) comparing the two batches of ginger seedlings obtained in the step (6).
Embodiment two:
ginger detoxification and probiotic combination protection cultivation, the production and application thereof are described in detail below:
step (1): washing off soil on the surface of ginger seeds by using underground water or tap water, soaking the ginger seeds in a washing liquid, washing the ginger seeds by using underground water, sending the ginger seeds into a detoxification room which is sterilized by a physical and chemical comprehensive method, and fumigating the ginger seeds, wherein the concentration of the washing liquid is 5%, and the soaking time of the ginger seeds in the washing liquid is 10 minutes;
step (II): the method comprises the steps of (1) carrying out germination accelerating treatment on a detoxification room, keeping the detoxification room at a relative humidity of 60%, and carrying out heat treatment at a temperature of 40 ℃ in daytime and 35 ℃ at night for 20 days, when a few terminal buds begin to germinate, spraying gibberellin liquid on the surface of ginger seeds, and carrying out treatment for one week to obtain a plurality of adventitious buds on the surface of ginger seeds, wherein the concentration of the gibberellin liquid is 50Mg/L, the humidity of the detoxification room is adjusted to 75% after the spraying, the temperature is adjusted to 23 ℃ in daytime and 20 ℃ at night;
step (III): a piece of tissue was cut every two shoots in units of adventitious shoots. Soaking cut ginger in 6-BA0.5mg/L+NAA0.1mg/L per liter, placing in a detoxification room for 1 hour, soaking ginger seeds in bacillus and added bacteria liquid, and then transferring into a greenhouse for cultivation, wherein the bacillus immersed in the ginger seeds is bacillus subtilis (in a probiotic single bacteria combination for disease prevention and control of ginger), and the soaked bacteria liquid is a comprehensive bacteria liquid of Japanese em bacteria;
step (IV): firstly, filling nutrition mud into which probiotics are implanted into a nutrition cup, then, embedding ginger seeds into the nutrition cup to a depth of 1MM (the standard that ginger blocks cannot be seen), then, watering with bacterial liquid which is prepared by jointly culturing 0.1% of ginger block extract and probiotics, enabling the humidity to be 75%, after 20 days, enabling ginger seedlings to come out of soil, then, culturing three true leaves, enabling root systems to be basically full of the nutrition cup, and controlling the temperature of a greenhouse to be about 25 ℃ and the humidity to be 70%.
Step (five): separating old tissue, adding thiofidaxl methyl into talcum powder, and then preparing sterilizing powder by combining chlorothalonil, wherein the concentration of the thiofidaxl methyl is 70%, and the concentration of the sterilizing powder prepared by the chlorothalonil is 75%;
step (six): ginger seedlings are transplanted into a field for cultivation, about 4600 plants are planted in each mu (667 square), and the old tissues are subjected to secondary greenhouse cultivation; obtaining two batches of ginger seedlings altogether;
step (seven): and (5) comparing the two batches of ginger seedlings obtained in the step (6).
To test the effect of the stiffening agents of the above examples, the following tests were carried out with the three groups of the agents described above:
pulping the slurry to 38+/-1 DEG SR by using domestic waste cartons as raw materials; adding reinforcing agent, stirring for 5 min, shaping by semi-automatic paper shaper, oven drying, naturally regulating water content for 24 hr, and detecting indexes such as burst and stiffness.
As shown in FIG. 1, and the above examples and test examples, the method has the advantage of simple operation, and the reinforcing agent synthesized by the method has good performance
It should also be noted that the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising one … …" does not exclude the presence of other like elements in a process, method, article or apparatus that comprises an element.
Although the present invention has been described with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications may be made to the embodiments described, or equivalents may be substituted for elements thereof, and any modifications, equivalents, improvements and changes may be made without departing from the spirit and principles of the present invention.
Claims (3)
1. The ginger detoxification and probiotics joint protection cultivation method is characterized by comprising the following specific steps of:
step (1): washing off soil on the surface of ginger seeds by using underground water or tap water, soaking the ginger seeds in a washing liquid, washing and cleaning the ginger seeds by using underground water, and sending the ginger seeds into a detoxification room which is sterilized by a physical and chemical comprehensive method, and then fumigating the ginger seeds;
step (II): the germination accelerating treatment is carried out on the detoxification room, the detoxification room is kept at the relative humidity of 60%, the temperature is 40 ℃ in the daytime and 35 ℃ at night, after the heat treatment for 15-20 days, when few terminal buds begin to germinate, the surface of ginger seeds is sprayed with gibberellin liquid, and a plurality of adventitious buds are generated on the surface of the ginger seeds after one week of treatment;
step (III): taking adventitious buds as units, cutting a piece of tissue every two buds; soaking cut ginger seeds in 6-BA0.5mg/L+NAA0.1mg/L, placing in a detoxification room for 1 hour, soaking ginger seeds in the comprehensive bacterial liquid of bacillus subtilis and Japanese em bacteria, and then transferring into a greenhouse for cultivation;
step (IV): firstly, filling nutrition mud into which probiotics are implanted into a nutrition cup, then embedding ginger seeds into the nutrition cup to a depth of 1mm, then watering with bacterial liquid which is jointly cultured by 0.1% of ginger block extract and probiotics, enabling ginger seedlings to come out of soil after 15 days, and then culturing until three true leaves are grown in root systems, wherein the root systems are basically full of the nutrition cup;
in the third step, the temperature of the greenhouse is controlled to be 23-25 ℃ and the humidity is 70%;
step (five): separating old tissue, adding thiophanate-methyl into talcum powder, and then treating wounds by using sterilizing powder prepared from chlorothalonil;
in the step (five), the concentration of the thiophanate-methyl is 70 percent, and the concentration of the sterilizing powder prepared from the chlorothalonil is 75 percent;
step (six): the ginger seedlings are transplanted into a field for cultivation, 4600 plants are planted per mu, and the old tissues are subjected to secondary greenhouse cultivation; obtaining two batches of ginger seedlings altogether;
step (seven): and (3) comparing the two batches of ginger seedlings obtained in the step (six).
2. The ginger detoxification and probiotic-combined protection cultivation according to claim 1, wherein: the concentration of the washing liquid in the step (one) is 5%, and the soaking time of the ginger seeds in the washing liquid is 10 minutes.
3. The ginger detoxification and probiotic-combined protection cultivation according to claim 1, wherein: in the step (II), the concentration of gibberellin liquid is 50mg/L, the humidity of the detoxification room after spraying is adjusted to 75%, and the temperature is adjusted to 23 ℃ in the daytime and 20 ℃ in the night.
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| CN101999285A (en) * | 2010-09-11 | 2011-04-06 | 六安市金土地农业科普示范园 | Method for cultivating gingers completely covered by rice straws and wheat straws |
| KR101653769B1 (en) * | 2014-09-25 | 2016-09-02 | 이성민 | Method cultivating a ginger |
| KR101659473B1 (en) * | 2014-12-05 | 2016-09-23 | 이준백 | A method of cultivation for ginger |
| CN104838857B (en) * | 2015-05-18 | 2017-02-01 | 张其录 | Organic fresh ginger planting method |
| CN106212277B (en) * | 2016-07-21 | 2018-06-22 | 广西壮族自治区农业科学院微生物研究所 | A kind of method for producing ginger mycorhiza tissue culture of sprout |
| CN109418108A (en) * | 2017-06-30 | 2019-03-05 | 郜秋华 | A kind of implantation methods of ginger |
| CN107624585A (en) * | 2017-10-31 | 2018-01-26 | 四川明峰农业开发有限公司 | A kind of ginger cultural method |
| CN107926568B (en) * | 2017-12-08 | 2020-05-12 | 吕诗林 | Ginger planting method |
| TWI712361B (en) * | 2018-06-01 | 2020-12-11 | 謝瑞裕 | Method of ginger plant cultivation |
| CN111742803B (en) * | 2020-06-19 | 2021-08-17 | 唐山市农业科学研究院 | Efficient cultivation method based on ginger micro-sprinkling irrigation water and fertilizer integration |
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