CN105830918B - A kind of method for improving serrate clubmoss herb gemma transplanting survival rate - Google Patents
A kind of method for improving serrate clubmoss herb gemma transplanting survival rate Download PDFInfo
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- CN105830918B CN105830918B CN201610186943.7A CN201610186943A CN105830918B CN 105830918 B CN105830918 B CN 105830918B CN 201610186943 A CN201610186943 A CN 201610186943A CN 105830918 B CN105830918 B CN 105830918B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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Abstract
The invention discloses a kind of method for improving serrate clubmoss herb gemma transplanting survival rate, this method obtains serrate clubmoss herb association fern original foliage using serrate clubmoss herb plant explant, serrate clubmoss herb association fern original foliage is transferred in moss matrix and cultivated, recycles the moss matrix for the original foliage for cultivating serrate clubmoss herb association fern to carry out the transplanting culture of serrate clubmoss herb gemma；The moss of the association fern isolated using cultivating from serrate clubmoss herb cultivates the gemma of serrate clubmoss herb, improves the transplanting survival rate of serrate clubmoss herb gemma.It is of the invention unique easy, provide important channel for the artificial propagation of serrate clubmoss herb.
Description
Technical field
The invention belongs to biological technical field, and in particular to kind is a kind of to improve serrate clubmoss herb (Huperzia serrata
(Thunb.) Trev.) gemma transplanting survival rate method.
Background technology
Serrate clubmoss herb (Huperzia serrata (Thunb.) Trev.) is Lycopodiales (Lycopodiales) Huperziaceae
(Lycopodiaceae) stone araucaria (Lycopodium L.) perennial herb pteridophyte.With eliminating stasis to subdue swelling, removing toxic substances and stop
The effect of pain.Nineteen twenty-seven China scientific worker, which reports the huperzine separated from the plant, has band muscular relaxation work
With numerous studies afterwards find that huperzine is a kind of efficient, less toxic, reversible and high selectivity acetylcholine ester enzyme level
Agent.Now, by huperzine and through the medicine as treatment Alzheimer disease (AD, Alzheimer disease), while
The U.S. is used as the nutrient and healthcare products of intelligence development.
But serrate clubmoss herb natural resources critical shortage.Serrate clubmoss herb is slow-growing, and the breeding of serrate clubmoss herb is main under wild state
Based on sporogenesis, spore germination cycle length, raw gametophyte under possession, needs 6-15 ripe after sprouting.Therefore greatly
Ground limits the regeneration of serrate clubmoss herb wild resource.At present, the extraction of huperzine depends on wild resource, and recovery rate is only
0.006%-0.1%.Therefore, market is very big to the demand of serrate clubmoss herb, and the natural resources for rapidly resulting in serrate clubmoss herb is in extinction
Edge.It is attempted to carry out the quick breeding of serrate clubmoss herb using tissue culture technique or the means of cuttage, but is all in progress
Slowly.
The content of the invention
It is an object of the present invention to provide a kind of method of serrate clubmoss herb gemma transplanting survival rate, this method can be carried significantly
High serrate clubmoss herb gemma transplanting survival rate, important channel is provided for the artificial propagation of serrate clubmoss herb.
In order to realize above-mentioned task, the present invention takes following technical solution：
A kind of method for improving serrate clubmoss herb gemma transplanting survival rate, this method obtain thousand layers using serrate clubmoss herb explant
Tower association fern original foliage, serrate clubmoss herb association fern original foliage is transferred to culture in moss matrix and obtains serrate clubmoss herb gemma transplanting moss
Matrix, serrate clubmoss herb gemma transplanting moss matrix is recycled to carry out the transplanting culture of serrate clubmoss herb gemma.
Specifically, described serrate clubmoss herb explant includes serrate clubmoss herb blade or serrate clubmoss herb stem.
More specifically, described moss matrix is the moss obtained after dry moss saturation absorbs water.
Further, described utilization serrate clubmoss herb explant, which obtains serrate clubmoss herb association fern original foliage, includes：Take outside serrate clubmoss herb
It is inoculated on MS solid mediums and is cultivated until growing serrate clubmoss herb association fern original foliage after implant sterilizing, culture environment temperature is
28 DEG C, culture environment intensity of illumination is 1000Lux, and the culture photoperiod is 12 hours.
Further, serrate clubmoss herb gemma transplants being included for moss matrix：It is 0.3~0.5cm by volume3Thousand layers
Tower association fern original foliage, which is transferred in moss matrix, to be cultivated, and trains moss matrix with the MS containing polyvinylpyrrolidone after one week
Continue to cultivate after nutrient solution filling saturation, culture environment humidity is 60%~75%, 25 DEG C of culture environment temperature, culture environment illumination
Intensity is 2000Lux, and the culture photoperiod is 8 hours, and serrate clubmoss herb gemma transplanting moss matrix is produced after continuing culture three months.
Specifically, in the MS nutrient solutions containing polyvinylpyrrolidone the concentration of polyvinylpyrrolidone for 50~
150mg/L。
In addition, the transplanting culture of moss matrix progress serrate clubmoss herb gemma is transplanted using serrate clubmoss herb gemma to be included：By serrate clubmoss herb
Gemma transplanting moss matrix transplants serrate clubmoss herb luxuriant after spraying saturation with ascorbic acid solution, then is trained with the MS containing indolebutyric acid
Nutrient solution sprays the culture that saturation serrate clubmoss herb gemma transplanting moss matrix carries out serrate clubmoss herb gemma, and culture environment humidity is 60%~
75%, culture environment temperature is 25 DEG C, and culture intensity of illumination is 2000Lux, cultivates 8 hours photoperiods.
Specifically, the concentration of described ascorbic acid solution is 150~250mg/L.
More specifically, indolebutyric acid concentration is 2~4mg/L in the MS nutrient solutions containing indolebutyric acid.
It is characteristic of the invention that：
1) culture of serrate clubmoss herb association fern original foliage is carried out using serrate clubmoss herb plant, serrate clubmoss herb association fern original foliage grew
Moss matrix culture gemma.Association fern is the plant with serrate clubmoss herb association in itself in natural habitat, in long-term existence
The material base of its shared promotion growth is formd in journey.The present invention was cleverly grown using serrate clubmoss herb association fern original foliage
Moss cultivate serrate clubmoss herb gemma, gemma is using the material secreted in association fern growth course because promote its growth；
2) polyvinylpyrrolidone, ascorbic acid and heteroauxin processing moss are utilized, hence it is evident that improve serrate clubmoss herb gemma
Transplanting efficiency；
3) original foliage of the association fern either still obtained with the blade of serrate clubmoss herb with the stem culture of serrate clubmoss herb, to thousand layers
The transplanting difference of tower gemma is little.Optimal parameter is：The concentration of polyvinylpyrrolidone is 100mg/L, ascorbic acid it is dense
Spend for 200mg/L, the concentration of ascorbic acid is 3mg/L.In such a situa-tion, the transplanting survival rate of serrate clubmoss herb gemma reaches
96%.
Brief description of the drawings
Fig. 1 is the concrete operations flow chart of the raising serrate clubmoss herb gemma transplanting survival rate of the present invention；
The present invention is illustrated below in conjunction with specification drawings and specific embodiments.
Embodiment
Gemma breeding is the common modes of reproduction of Huperziaceae plant, is that the one kind being grown on plant is nourished and generated, external
Have been reported the formation mechenism of gemma, breeding effect etc..Wang Deli etc. (2011, Agriculture of Anhui science) is tentatively to the bud of serrate clubmoss herb
Spore transplanting is studied, but reproductive efficiency is not very high.Inventor is on the basis of early-stage Study, from serrate clubmoss herb explant
Turn out association fern original foliage, recycle and grew the moss of association fern original foliage to cultivate the gemma of serrate clubmoss herb, such gemma
The survival rate of transplanting significantly improves.
With reference to Fig. 1, the method for raising serrate clubmoss herb Explant surface sterilizing efficiency of the invention, operate according to the following steps：
Step 1, the acquisition of serrate clubmoss herb association fern original foliage：
The blade or stem of serrate clubmoss herb are taken, surface sterilizing is carried out with conventional plant explant sterilization, is then seeded into MS
On solid medium, the culture that is placed into culturing room, condition of culture is：Temperature is 28 DEG C, intensity of illumination 1000Lux, light
12 hours cycles.After culture 2 months, the original foliage of association fern is grown from serrate clubmoss herb explant edge.
Step 2, the acquisition of the moss matrix for cultivating serrate clubmoss herb gemma：
It is 0.3~0.5cm to treat original foliage length to volume3When, it is transferred in moss matrix.With containing 50 after one week
After the MS nutrient solutions of~150mg/L polyvinylpyrrolidones spray saturation matrix, then it is placed into culturing room's culture.Condition of culture
For：Humidity is 60%~75%, 25 DEG C of temperature, intensity of illumination 2000Lux, photoperiod 8 hours.In incubation, with originally
Water pours, to ensure that moss moistens.Moss after cultivating 30 days can be used for the transplanting of serrate clubmoss herb gemma.
Step 3, the transplanting of serrate clubmoss herb gemma：
The moss matrix 150~250mg/L ascorbic acid solutions sprinkling wetting saturation for having association fern original foliage will be cultivated,
The gemma of serrate clubmoss herb is transplanted in wherein, then saturation matrix is sprayed with the MS nutrient solutions containing 2~4mg/L indolebutyric acids, then
It is positioned over culturing room's culture.Condition of culture is：Humidity is 60%~75%, and temperature is 25 DEG C, intensity of illumination 2000Lux, light
8 hours cycles.In incubation, poured with running water, to ensure that moss moistens.Counted after 60 days into the quantity of living spores,
And calculate transplanting survival rate.
MS culture mediums form：1.65g/L NH4NO3, 1.9g/L KNO3, 0.17g/L KH2PO4, 0.37g/L
MgSO47H2O, 0.44g/L CaCl22H2O, 0.83mg/L KI, 6.2mg/L H3BO3, 22.3mg/L MnSO44H2O、
8.6mg/L ZnSO47H2O, 0.25mg/L Na2MoO42H2O, 0.025mg/L CuCl25H2O, 0.025mg/L
CoCl26H2O, 37.3mg/L Na2EDTA, 27.8mg/L FeSO47H2O。
Here each composition is calculated according to every liter of liquid, MS be than more conventional culture medium in tissue cultures, if
It is solidified MS media, agar powder is added in mentioned component；If MS nutrient solutions are prepared according to mentioned component, contain
There are polyvinylpyrrolidone MS nutrient solutions to add polyvinylpyrrolidone i.e. in original MS nutrient solutions to produce, contain indoles fourth
The MS nutrient solutions of acid add indolebutyric acid in original MS nutrient solutions and produced.
Moss matrix is the drying moss bought on the market, the moss obtained after running water fully absorbs water saturation, with
Usually the moss discrete phase used of raising flowers is same.
Transplanting survival rate refers to：After serrate clubmoss herb gemma transplanting moss matrix culture 60 days, the gemma number taken root
Amount accounts for the percentage of total gemma number.
The conventional surface sterilizing of serrate clubmoss herb explant includes：The dirty of serrate clubmoss herb explant surface first is rinsed with running water,
Then on superclean bench with alcohol 30~60s of surface sterilizing that volume fraction is 70%, then with volume fraction it is 0.1%
Mercuric chloride solution sterilizes 8 minutes, finally with 3~5 surface sterilizings for completing serrate clubmoss herb explant of aseptic water washing
The present invention is illustrated below in conjunction with instantiation：
Embodiment 1：
The gemma of serrate clubmoss herb is transplanted in the moss matrix being sufficiently humidified so as to running water, is then placed into culturing room's training
Support.Condition of culture is：Humidity is more than 75%, 25 DEG C of temperature, intensity of illumination 2000Lux, photoperiod 8 hours.In incubation,
Poured with running water, to ensure that moss moistens.The transplanting survival rate of serrate clubmoss herb gemma is 32% after 60 days.
Embodiment 2：
The gemma of serrate clubmoss herb is transplanted in the moss matrix sprayed with the MS nutrient solutions containing 3mg/L indolebutyric acids,
It is then placed into culturing room's culture.Condition of culture is：Humidity is more than 75%, and 25 DEG C of temperature, intensity of illumination 2000Lux, light are all
8 hours phases.In incubation, poured with running water, to ensure that moss moistens.The transplanting survival rate of serrate clubmoss herb gemma is after 60 days
46%.
Embodiment 3：
Step 1, the acquisition of association fern original foliage in serrate clubmoss herb：
The blade of serrate clubmoss herb is taken, surface sterilizing is carried out with conventional plant explant sterilization, then by the thousand of surface sterilizing
Layer tower blade inoculation be to the culture on MS solid mediums, being placed into culturing room, condition of culture：Temperature is 28 DEG C, illumination
Intensity is 1000Lux, 12 hours photoperiods.After culture 2 months, the original foliage of association fern is grown from serrate clubmoss herb blade edge.
Step 2, the acquisition of the moss matrix for cultivating serrate clubmoss herb gemma：
Treat original foliage length to 0.5cm3More than, it is transferred in moss matrix and is cultivated.Condition of culture is：Humidity is more than
75%, 25 DEG C of temperature, intensity of illumination 2000Lux, photoperiod 8 hours.In incubation, poured with running water, to ensure green grass or young crops
Tongue moistens.Moss after cultivating 30 days can be used for the transplanting of serrate clubmoss herb gemma.
Step 3, the transplanting of serrate clubmoss herb gemma：
The gemma of serrate clubmoss herb is transplanted in the moss matrix for having serrate clubmoss herb prophyll to grow, is then placed into culturing room's training
Support.Condition of culture is：Humidity is more than 75%, 25 DEG C of temperature, intensity of illumination 2000Lux, photoperiod 8 hours.In incubation,
Poured with running water, to ensure that moss moistens.
The transplanting survival rate of serrate clubmoss herb gemma is 57% after 60 days.
Embodiment 4：
Step 1, the acquisition of association fern original foliage in serrate clubmoss herb：
The blade of serrate clubmoss herb is taken, surface sterilizing is carried out with conventional plant explant sterilization, is then seeded into the training of MS solids
Support on base, the culture being placed into culturing room, condition of culture is：Temperature is 28 DEG C, intensity of illumination 1000Lux, the photoperiod 12
Hour.After culture 2 months, the original foliage of association fern is grown from serrate clubmoss herb blade edge.
Step 2, the acquisition of the moss matrix for cultivating serrate clubmoss herb gemma：
Treat original foliage length to 0.5cm3More than, it is transferred in moss matrix.Used after one week and contain the poly- second of 50mg/L
The MS nutrient solutions of alkene pyrrolidone spray once, then are placed into culturing room's culture.Condition of culture is：Humidity is more than 75%, temperature
25 DEG C, intensity of illumination 2000Lux, photoperiod 8 hours.In incubation, poured with running water, to ensure that moss moistens.Training
Moss after supporting 30 days can be used for the transplanting of serrate clubmoss herb gemma.
Step 3, the transplanting of serrate clubmoss herb gemma：
The moss 150mg/L ascorbic acid sprinkling wetting for having association fern original foliage, then the gemma by serrate clubmoss herb will be cultivated
Transplant in wherein, sprayed once with the MS nutrient solutions containing 2mg/L indolebutyric acids, be then placed into culturing room's culture.Cultivate bar
Part is：Humidity is more than 75%, 25 DEG C of temperature, intensity of illumination 2000Lux, photoperiod 8 hours.In incubation, running water is used
Pour, to ensure that moss moistens.
The transplanting survival rate of serrate clubmoss herb gemma is 73% after 60 days.
Embodiment 5：
Step 1：With embodiment 4.
Step 2, the acquisition of the moss matrix for cultivating serrate clubmoss herb gemma：
Treat original foliage length to 0.5cm3More than, it is transferred in moss matrix.Used after one week and contain the poly- second of 100mg/L
The MS nutrient solutions of alkene pyrrolidone spray once, then are placed into culturing room's culture.Condition of culture is：Humidity is more than 75%, temperature
25 DEG C, intensity of illumination 2000Lux, photoperiod 8 hours.In incubation, poured with running water, to ensure that moss moistens.Training
Moss after supporting 30 days can be used for the transplanting of serrate clubmoss herb gemma.
Step 3, the transplanting of serrate clubmoss herb gemma：
The moss 200mg/L ascorbic acid sprinkling wetting for having association fern original foliage, then the gemma by serrate clubmoss herb will be cultivated
Transplant in wherein, sprayed once with the MS nutrient solutions containing 3mg/L indolebutyric acids, be then placed into culturing room's culture.Cultivate bar
Part is：Humidity is more than 75%, 25 DEG C of temperature, intensity of illumination 2000Lux, photoperiod 8 hours.In incubation, running water is used
Pour, to ensure that moss moistens.
The transplanting survival rate of serrate clubmoss herb gemma is 96% after 60 days.
Embodiment 6：
Step 1：With embodiment 4.
Step 2, the acquisition of the moss matrix for cultivating serrate clubmoss herb gemma：
Treat original foliage length to 0.5cm3More than, it is transferred in moss matrix.Used after one week and contain the poly- second of 100mg/L
The MS nutrient solutions of alkene pyrrolidone spray once, then are placed into culturing room's culture.Condition of culture is：Humidity is more than 75%, temperature
25 DEG C, intensity of illumination 2000Lux, photoperiod 8 hours.In incubation, poured with running water, to ensure that moss moistens.Training
Moss after supporting 30 days can be used for the transplanting of serrate clubmoss herb gemma.
Step 3, the transplanting of serrate clubmoss herb gemma：
The moss 250mg/L ascorbic acid sprinkling wetting for having association fern original foliage, then the gemma by serrate clubmoss herb will be cultivated
Transplant in wherein, sprayed once with the MS nutrient solutions containing 4mg/L indolebutyric acids, be then placed into culturing room's culture.Cultivate bar
Part is：Humidity is more than 75%, 25 DEG C of temperature, intensity of illumination 2000Lux, photoperiod 8 hours.In incubation, running water is used
Pour, to ensure that moss moistens.
The transplanting survival rate of serrate clubmoss herb gemma is 80% after 60 days.
Embodiment 7：
Step 1：With embodiment 4.
Step 2, the acquisition of the moss matrix for cultivating serrate clubmoss herb gemma：
Treat original foliage length to 0.5cm3More than, it is transferred in moss matrix.Used after one week and contain the poly- second of 150mg/L
The MS nutrient solutions of alkene pyrrolidone spray once, then are placed into culturing room's culture.Condition of culture is：Humidity is more than 75%, temperature
25 DEG C, intensity of illumination 2000Lux, photoperiod 8 hours.In incubation, poured with running water, to ensure that moss moistens.Training
Moss after supporting 30 days can be used for the transplanting of serrate clubmoss herb gemma.
Step 3, the transplanting of serrate clubmoss herb gemma：
The moss 200mg/L ascorbic acid sprinkling wetting for having association fern original foliage, then the gemma by serrate clubmoss herb will be cultivated
Transplant in wherein, sprayed once with the MS nutrient solutions containing 3mg/L indolebutyric acids, be then placed into culturing room's culture.Cultivate bar
Part is：Humidity is more than 75%, 25 DEG C of temperature, intensity of illumination 2000Lux, photoperiod 8 hours.In incubation, running water is used
Pour, to ensure that moss moistens.
The transplanting survival rate of serrate clubmoss herb gemma is 85% after 60 days.
Embodiment 8：
Step 1, the acquisition of association fern original foliage in serrate clubmoss herb：
The stem of serrate clubmoss herb is taken, surface sterilizing is carried out with conventional plant explant sterilization, is then seeded into MS solid cultures
On base, the culture that is placed into culturing room, condition of culture is：Temperature is 28 DEG C, intensity of illumination 1000Lux, and the photoperiod 12 is small
When.After culture 2 months, the original foliage of association fern is grown from serrate clubmoss herb stem edge.
Step 2, the acquisition of the moss matrix for cultivating serrate clubmoss herb gemma：
Treat original foliage length to 0.5cm3More than, it is transferred in moss matrix.Used after one week and contain the poly- second of 50mg/L
The MS nutrient solutions of alkene pyrrolidone spray once, then are placed into culturing room's culture.Condition of culture is：Humidity is more than 75%, temperature
25 DEG C, intensity of illumination 2000Lux, photoperiod 8 hours.In incubation, poured with running water, to ensure that moss moistens.Training
Moss after supporting 30 days can be used for the transplanting of serrate clubmoss herb gemma.
Step 3, the transplanting of serrate clubmoss herb gemma：
The moss 150mg/L ascorbic acid sprinkling wetting for having association fern original foliage, then the gemma by serrate clubmoss herb will be cultivated
Transplant in wherein, sprayed once with the MS nutrient solutions containing 4mg/L indolebutyric acids, be then placed into culturing room's culture.Cultivate bar
Part is：Humidity is more than 75%, 25 DEG C of temperature, intensity of illumination 2000Lux, photoperiod 8 hours.In incubation, running water is used
Pour, to ensure that moss moistens.
The transplanting survival rate of serrate clubmoss herb gemma is 73% after 60 days.
Embodiment 9：
With embodiment 5, the stem that the blade of serrate clubmoss herb in step 1 is changed into serrate clubmoss herb is a difference in that.
The transplanting survival rate of serrate clubmoss herb gemma is 93%.
Embodiment 10：
Step 1：With embodiment 8.
Step 2, the acquisition of the moss matrix for cultivating serrate clubmoss herb gemma：
Treat original foliage length to 0.5cm3More than, it is transferred in moss matrix.Used after one week and contain the poly- second of 150mg/L
The MS nutrient solutions of alkene pyrrolidone spray once, then are placed into culturing room's culture.Condition of culture is：Humidity is more than 75%, temperature
25 DEG C, intensity of illumination 2000Lux, photoperiod 8 hours.In incubation, poured with running water, to ensure that moss moistens.Training
Moss after supporting 30 days can be used for the transplanting of serrate clubmoss herb gemma.
Step 3, the transplanting of serrate clubmoss herb gemma：
The moss 250mg/L ascorbic acid sprinkling wetting for having association fern original foliage, then the gemma by serrate clubmoss herb will be cultivated
Transplant in wherein, sprayed once with the MS nutrient solutions containing 2mg/L indolebutyric acids, be then placed into culturing room's culture.Cultivate bar
Part is：Humidity is more than 75%, 25 DEG C of temperature, intensity of illumination 2000Lux, photoperiod 8 hours.In incubation, running water is used
Pour, to ensure that moss moistens.
The transplanting survival rate of serrate clubmoss herb gemma is 78% after 60 days.
Claims (8)
- A kind of 1. method for improving serrate clubmoss herb gemma transplanting survival rate, it is characterised in that this method is using serrate clubmoss herb explant Body obtains serrate clubmoss herb association fern original foliage, and serrate clubmoss herb association fern original foliage is transferred into culture in moss matrix obtains serrate clubmoss herb bud Spore transplants moss matrix, recycles serrate clubmoss herb gemma transplanting moss matrix to carry out the transplanting culture of serrate clubmoss herb gemma；Serrate clubmoss herb gemma transplanting moss matrix is included：It is 0.3~0.5cm by volume3Serrate clubmoss herb association fern original foliage turn It is connected in moss matrix and cultivates, it is after one week that moss matrix is follow-up with the MS nutrient solutions filling saturation containing polyvinylpyrrolidone Continuous culture, culture environment humidity are 60%~75%, and 25 DEG C of culture environment temperature, culture environment intensity of illumination is 2000Lux, training It is 8 hours to support the photoperiod, and serrate clubmoss herb gemma transplanting moss matrix is produced after continuing culture 30 days.
- 2. the method for serrate clubmoss herb gemma transplanting survival rate is improved as claimed in claim 1, it is characterised in that described serrate clubmoss herb Explant includes serrate clubmoss herb blade or serrate clubmoss herb stem.
- 3. the method for serrate clubmoss herb gemma transplanting survival rate is improved as claimed in claim 1 or 2, it is characterised in that described green grass or young crops Tongue matrix is the moss obtained after dry moss saturation absorbs water.
- 4. the method for serrate clubmoss herb gemma transplanting survival rate is improved as claimed in claim 1 or 2, it is characterised in that described profit Obtaining serrate clubmoss herb association fern original foliage with serrate clubmoss herb explant includes：Serrate clubmoss herb explant is taken to be inoculated into MS solid cultures after sterilizing Until growing serrate clubmoss herb association fern original foliage, culture environment temperature is 28 DEG C for culture on base, and culture environment intensity of illumination is 1000Lux, culture photoperiod are 12 hours.
- 5. the method for serrate clubmoss herb gemma transplanting survival rate is improved as claimed in claim 1, it is characterised in that described containing is poly- The concentration of polyvinylpyrrolidone is 50~150mg/L in vinylpyrrolidone MS nutrient solutions.
- 6. the method for serrate clubmoss herb gemma transplanting survival rate is improved as claimed in claim 1 or 2, it is characterised in that utilize thousand layers The transplanting culture that tower gemma transplanting moss matrix carries out serrate clubmoss herb gemma includes：By serrate clubmoss herb gemma transplanting moss matrix with anti-bad Hematic acid solution transplants serrate clubmoss herb luxuriant after spraying saturation, then sprays saturation serrate clubmoss herb gemma with the MS nutrient solutions containing indolebutyric acid The culture that moss matrix carries out serrate clubmoss herb gemma is transplanted, culture environment humidity is 60%~75%, and culture environment temperature is 25 DEG C, Culture intensity of illumination is 2000Lux, cultivates 8 hours photoperiods.
- 7. the method for serrate clubmoss herb gemma transplanting survival rate is improved as claimed in claim 6, it is characterised in that described Vitamin C The concentration of acid solution is 150~250mg/L.
- 8. the method for serrate clubmoss herb gemma transplanting survival rate is improved as claimed in claim 6, it is characterised in that described contains Yin Indolebutyric acid concentration is 2~4mg/L in the MS nutrient solutions of diindyl butyric acid.
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| CN107267436B (en) * | 2017-07-04 | 2021-07-13 | 西北大学 | Preparation method of huperzia serrata leaf protoplast |
| CN109526710B (en) * | 2018-07-26 | 2021-04-30 | 皮剑波 | Huperzia serrata and soilless culture method thereof |
| CN109258148A (en) * | 2018-09-29 | 2019-01-25 | 西北大学 | A kind of method of Huperzia serrata rooting of cuttings |
| CN110551635B (en) * | 2019-07-29 | 2021-09-28 | 西北大学 | Huperzia serrata endophytic green algae and application thereof |
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| CN104304025B (en) * | 2014-10-24 | 2016-01-20 | 西北大学 | The artificial fecundation method of a kind of narrow leaf Parathelypteris nipponica |
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