CN107047617A - Low temperature abscisic acid and its application method - Google Patents
Low temperature abscisic acid and its application method Download PDFInfo
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- CN107047617A CN107047617A CN201710290359.0A CN201710290359A CN107047617A CN 107047617 A CN107047617 A CN 107047617A CN 201710290359 A CN201710290359 A CN 201710290359A CN 107047617 A CN107047617 A CN 107047617A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/36—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids
- A01N37/38—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids having at least one oxygen or sulfur atom attached to an aromatic ring system
- A01N37/40—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids having at least one oxygen or sulfur atom attached to an aromatic ring system having at least one carboxylic group or a thio analogue, or a derivative thereof, and one oxygen or sulfur atom attached to the same aromatic ring system
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N61/00—Biocides, pest repellants or attractants, or plant growth regulators containing substances of unknown or undetermined composition, e.g. substances characterised only by the mode of action
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Abstract
The present invention discloses a kind of low temperature abscisic acid and its application method, and category strengthens the abscisic acid field of plant resistance to environment stress, and it ferments chelatase, salicylic acid, the freeze proof immune factors of Glucose according to 1 by selected Xanthohumate, import：1.5‑2：1.5‑2：2.5 3 weight is made than well mixed.The application method of low temperature abscisic acid, comprises the following steps：A, getting fat strong sprout：Autumn, to fruit strain organic fertilizer, phosphorus potash fertilizer, improves carbon-nitrogen rate；B, ferment are stimulated：From late October, the environmental protection ferment being once made after the useless bud flowers and fruits, brown sugar, water by fermentation of same seed pod strain was applied every 12 18 days, it is continuous to use 3 stimulation plant hormones；C, spray low temperature abscisic acid：1 is pressed in each cooling within first 58 days:600 ratio is diluted with water low temperature abscisic acid, then uniform sprinkling plant.The present invention improves cold tolerance, improves vegetables and fruits quality increase yield, and induction produces resistance, increases cold resistance.
Description
Technical field
It is more particularly to a kind of to lure anti-for fruit tree, the low temperature of fruit strain the present invention relates to a kind of abscisic acid and its application method
Element and its application method.
Background technology
Fruit such as dragon fruit, sugarcane, jackfruit etc. of some happiness heat, originate in tropical and subtropical region.With some lifes
Thing characteristic：It is moisture resistance, heat-resisting, not low temperature resistant etc..Such as dragon fruit is resistant to 40-50 DEG C of high temperature, and then has different journeys below 8 degrees Celsius
The cold damage of degree, can freeze to death less than 0 DEG C；Then there is different degrees of cold damage below 5 degrees Celsius of jackfruit, can be freezed to death less than 0 DEG C；Because
The nutrition and taste of these fruit are deep to be favored by market, and some southern provinces and cities introduce a fine variety one after another, and carry out cultivation experiments using greenhouse.
But some areas are survived the winter using monofilm, two-membrane process, still Yield and quality is undesirable.Lacking one kind can effective activated plant body
The factor of the interior degeneration-resistant immune system of cold-resistant.
The content of the invention
It is an object of the invention to provide a kind of low temperature abscisic acid and its application method, resistance, increase are produced to induction fruit strain
Cold resistance；Improve vegetables and fruits quality increase yield, produce resistance.
The present invention proposes a kind of low temperature abscisic acid, it is characterised in that by selected Xanthohumate, import fermentation chelatase, water
Poplar acid, the freeze proof immune factors of Glucose are according to 1：1.5-2：1.5-2：2.5-3 weight is made than well mixed.
The present invention also proposes a kind of application method of above-mentioned low temperature abscisic acid, comprises the following steps：
A, getting fat strong sprout：In October in autumn, an organic fertilizer is applied to fruit strain, then apply a phosphorus potash fertilizer, improve carbon-nitrogen rate；
B, ferment are stimulated：Entered before the winter, from late October, applied every 12-18 days once by same seed pod strain useless bud flowers and fruits,
The environmental protection ferment being made after brown sugar, water by fermentation, by 1:1000 ratio adds water and is diluted rear drip irrigation, pours and apply or foliage dressing,
It is continuous to use 3 stimulation plant hormones；
C, spray low temperature abscisic acid：Press 1 within 5-8 days before each cooling:600 ratio is diluted with water low temperature abscisic acid, then
Even sprinkling plant, until there is droplet.
Preferably, in described " B, ferment are stimulated " step, the environmental protection ferment preparation method is：Utilize the tender of same seed pod
Petal shape fruit, insect pest cull fruit and brown sugar, water under bud, flower and fruit thinning are thin is according to 2-3:1:8-10 weight ratio is mixed and put into
In one nonmetallic sealing container, partial containers space is reserved, bung is opened and deflate and stir within every 2 days the 1st month, from
Sealing container is statically placed in shady place and produced within 2-3 months.
Low temperature abscisic acid whole winter applies 3-4 times, when temperature is reduced to 5 DEG C or less, it is impossible to spray.
The beneficial effects of the invention are as follows：(1) this low temperature abscisic acid, can induce fruit strain to produce resistance, increase cold resistance, have
Degeneration-resistant immune system in activated plant body is imitated, strengthens comprehensive plant resistance, particularly cold tolerance.In environment stress, this is low
Warm abscisic acid is in intercellular trafficking adverse circumstance information, and induction plant part produces the resistivity of various replies, in cold stress
Abscisic acid active cell, which resists, produces Coldproof protein matter and the participation regulation of 150 several genes in cold gene, induction plant, enhancing is anti-
Cold ability.A variety of enzymatic conversion synzyme in antifreeze film, and activated plant body are formed on dragon fruit bough after, are effectively improved
Plant polyoses content, induced low temperature stress protein matter accumulation increases branch plumpness, enhancing insulation, water conservation and freezing tolerance increase
Heating amount, reduces icing ability, improves the tolerable temperature of plant pair low temperature, substantially reduces plant freezing point, improves plant pair low temperature
Resistance, mitigate harm of the freeze injury to plant, while accelerating Nutrient Absorption and operating, increase chlorophyll content, to making produce
Amount and quality are significantly increased.Xanthohumate be it is a kind of be one extracted from low level coal or plant fermentation waste material
Aromatic substance is planted, is also the new high-efficiency plant growth regulator for significantly improving disease resistance of plant, is lived with stronger chemistry
Property, promoting plant growth, improving crop anti-adversity energy, improving crop quality, promote nutrient absorption to utilize, subtract to pesticide synergistic
It can improve the activity of root activity and a variety of enzymes for the effect such as poison, promote absorption, operating of the plant to nutrient and trace element, increase
Plus the content and rate of photosynthisis of chlorophyll, yield and improving quality are improved, being worth internal peroxidase activity increase by raising plants
Thing resistance against diseases；It can be used in mixed way with various sterilization agent in addition, have great role to improving the yield and quality.Salicylic acid is being planted
Effect in thing disease resistance, and the angle Primary Study that is interacted from salicylic acid and hydrogen peroxide and its metabolic enzymes bigcatkin willow
The molecule mechanism of acid induction disease resistance of plant, salicylic acid (SA) is the signaling molecule of inducing plant resistance, can be by inducing plant
Producing the approach such as pathogenesis-related proteins (PR albumen), the related defence enzyme activity of regulation makes plant generation system acquired resistance
(SAR) after, SA is combined with the salicylic acid receptor albumen (SABP) with catalase (CAT) activity, intracellular peroxide is caused
Change resistant gene in the rise of hydrogen (H2O2) concentration, H2O2 energy activated plant bodies；And promotion of the Xanthohumate to peroxidase activity
And raising, it can further promote hydrogen peroxide (H2O2) concentration to raise, resistant gene in activated plant body.Chlorophyll is that plant enters
The photosynthetic primary pigments molecule of row, is also tetrapyrrole most abundant in plant, and the chelatase that ferments refers mainly to hair
The magnesium ion sequestration enzyme of ferment, magnesium ion sequestration enzyme is first enzyme of Chlorophyll synthesis branch approach, is also key enzyme, it can be urged
Change magnesium ion sequestration and magnesium protoporphysin Ⅸ is formed into protoporphysin Ⅸ, photosynthesis, healthy and strong plant can be promoted；And magnesium ion chela
The heterologous polymerase that synthase is made up of three subunits of I, D and H (CHLI, CHLD and CHLH), each subunit of magnesium ion sequestration enzyme is removed
Outside the chelating for participating in catalysis magnesium ion, other biological functions are also actively participated in, are interacted with other albumen, most
Limits the freeze proof immuno-biology function of the freeze proof immune factors of Glucose is ejected.
(1) October in autumn, getting fat strong sprout was that aftermentioned application low temperature abscisic acid is laid to fruit strain organic fertilizer, phosphorus potash fertilizer
Nutrition foundation.Autumn is the period of root growth, applies the biological organic fertilizer once fully become thoroughly decomposed mid-October, and increase soil has
Machine matter, improves soil environment, improves root activity.In increasing application phosphorus potassium fertilizer in October, carbon-nitrogen rate can be improved, phosphorus is in plant internal reference
With photosynthesis, respiration, energy storage and transmission, cell division, cell increase and some other process, phosphorus can promote morning
The formation and growth of phase root system, improve the ability that plant adapts to external environmental condition, contribute to the resistance to severe cold for spending winter of plant, have
Accelerating is acted on and enhancing disease resistance of plant.Potassium can promote photosynthesis, and potassium deficiency weakens photosynthesis；Potassium can be improved significantly
Absorption and utilization of the plant to nitrogen, and protein is converted into quickly；Another feature of potassium contributes to crop anti-adversity, it
One of important physiological action is the adjustment effect for strengthening cell to environmental condition.
(3) by homemade environmental protection ferment drip irrigation, pour apply or foliage dressing carry out ferment stimulation, plant He Er can be stimulated
Cover, training source is consolidated, degeneration-resistant immune system in plant is in stress situation, be that aftermentioned low temperature abscisic acid lays biological basis.
Homemade environmental protection ferment mainly using the dragon fruit tender shoots of this plant and neighbouring strain, flower and fruit thinning dredge lower petal shape really, insect pest it is residual
Inferior fruit is obtained with brown sugar, water by trimestral fermentation, and the various biology enzymes and gene after this plant of luxuriant fruit fermentation are active,
Brown sugar is just to refine sugar, and the inside contains the primitive components such as more mineral matter and is substantially free of adding ingredient, and brown sugar is also micro- in addition
Biological nutrition foundation, sugaring can accelerate fermentation, thoroughly, and ferment is more stable, environmental protection ferment by drip irrigation, pour and apply or blade face is applied
Fertilizer can stimulate plant hormone, improve vegetables and fruits quality increase yield, produce resistance.
The present invention cultivates healthy and strong aging tree body before the winter, promotes nutrient accumulation, improves cold tolerance, can stimulate plant lotus
You cover, and improve vegetables and fruits quality increase yield, produce resistance；Induction produces resistance, increases cold resistance.Such as the dragon fruit present invention
Afterwards, artificial heating can be not required in the relatively low south China northern area of China's winter temperature, monofilm covering is taken with regard to energy safe overwintering
The per mu yield for the dragon fruit next year that the inventive method is survived the winter is 5000 kilograms, to be increased compared with the dragon fruit next year per mu yield that two-membrane process is passed the winter
Production about 2%, two-membrane process needs to spend a large amount of human and material resources, than every mu of member of increase cost more than 1000 of the present invention, is unfavorable for scale
Metaplasia is produced.
Embodiment
Embodiment one
The low temperature abscisic acid of the present embodiment, it is characterised in that by selected Xanthohumate, import ferment chelatase, salicylic acid,
The freeze proof immune factors of Glucose are according to 1：1.6：1.6：2.5 weight is made than well mixed.
The present invention also proposes a kind of application method of above-mentioned low temperature abscisic acid, comprises the following steps：
A, getting fat strong sprout：In October in autumn, an organic fertilizer is applied to fruit strain, then apply a phosphorus potash fertilizer, improve carbon-nitrogen rate；
B, ferment are stimulated：Entered before the winter, from late October, the useless bud flowers and fruits, red once by the strain of same seed pod were applied every 13 days
The environmental protection ferment being made after sugar, water by fermentation, by 1:1000 ratio adds water and is diluted rear drip irrigation, pours and apply or foliage dressing, even
It is continuous to use 3 stimulation plant hormones；
C, spray low temperature abscisic acid：1 is pressed in each cooling within first 6 days:600 ratio is diluted with water low temperature abscisic acid, then uniformly
Plant is sprayed, until there is droplet.
In " the B, ferment are stimulated " step, the environmental protection ferment preparation method is：Dredged using the tender shoots of same seed pod, flower thinning
Petal shape fruit, insect pest cull fruit and brown sugar, water under fruit is thin is according to 2:1:8.5 weight is more nonmetallic than mixing and putting into one
In sealing container, partial containers space is reserved, bung is opened and deflate and stir within every 2 days the 1st month, will be close from 2-3 months
Envelope container is statically placed in shady place and produced.
Low temperature abscisic acid whole winter applies 3-4 times, when temperature is reduced to 5 DEG C or less, it is impossible to spray.Because branch
The frost critical-temperature of bar is at 2-3 DEG C, and increase liquid increases the probability endured cold on the contrary.
Embodiment two
The low temperature abscisic acid of the present embodiment, it is characterised in that by selected Xanthohumate, import ferment chelatase, salicylic acid,
The freeze proof immune factors of Glucose are according to 1：1.8：1.8：2.8 weight is made than well mixed.
The present invention also proposes a kind of application method of above-mentioned low temperature abscisic acid, comprises the following steps：
A, getting fat strong sprout：In October in autumn, an organic fertilizer is applied to fruit strain, then apply a phosphorus potash fertilizer, improve carbon-nitrogen rate；
B, ferment are stimulated：Entered before the winter, from late October, the useless bud flowers and fruits, red once by the strain of same seed pod were applied every 15 days
The environmental protection ferment being made after sugar, water by fermentation, by 1:1000 ratio adds water and is diluted rear drip irrigation, pours and apply or foliage dressing, even
It is continuous to use 3 stimulation plant hormones；
C, spray low temperature abscisic acid：1 is pressed in each cooling within first 7 days:600 ratio is diluted with water low temperature abscisic acid, then uniformly
Plant is sprayed, until there is droplet.
In " the B, ferment are stimulated " step, the environmental protection ferment preparation method is：Dredged using the tender shoots of same seed pod, flower thinning
Petal shape fruit, insect pest cull fruit and brown sugar, water under fruit is thin is according to 2.5:1:9 weight is more nonmetallic than mixing and putting into one
In sealing container, partial containers space is reserved, bung is opened and deflate and stir within every 2 days the 1st month, will be close from 2-3 months
Envelope container is statically placed in shady place and produced.
Low temperature abscisic acid whole winter applies 3-4 times, when temperature is reduced to 5 DEG C or less, it is impossible to spray.
Embodiment three
The low temperature abscisic acid of the present embodiment, it is characterised in that by selected Xanthohumate, import ferment chelatase, salicylic acid,
The freeze proof immune factors of Glucose are according to 1：2：2：3 weight is made than well mixed.
The present invention also proposes a kind of application method of above-mentioned low temperature abscisic acid, comprises the following steps：
A, getting fat strong sprout：In October in autumn, an organic fertilizer is applied to fruit strain, then apply a phosphorus potash fertilizer, improve carbon-nitrogen rate；
B, ferment are stimulated：Entered before the winter, from late October, the useless bud flowers and fruits, red once by the strain of same seed pod were applied every 16 days
The environmental protection ferment being made after sugar, water by fermentation, by 1:1000 ratio adds water and is diluted rear drip irrigation, pours and apply or foliage dressing, even
It is continuous to use 3 stimulation plant hormones；
C, spray low temperature abscisic acid：1 is pressed in each cooling within first 8 days:600 ratio is diluted with water low temperature abscisic acid, then uniformly
Plant is sprayed, until there is droplet.
In " the B, ferment are stimulated " step, the environmental protection ferment preparation method is：Dredged using the tender shoots of same seed pod, flower thinning
Petal shape fruit, insect pest cull fruit and brown sugar, water under fruit is thin is according to 3:1:9.5 weight is more nonmetallic than mixing and putting into one
In sealing container, partial containers space is reserved, bung is opened and deflate and stir within every 2 days the 1st month, will be close from 2-3 months
Envelope container is statically placed in shady place and produced.
Low temperature abscisic acid whole winter applies 3-4 times, when temperature is reduced to 5 DEG C or less, it is impossible to spray.
The preferred embodiments of the present invention are the foregoing is only, are not intended to limit the scope of the invention, it is every to utilize
The equivalent structure transformation that present specification is made, or other related technical fields are directly or indirectly used in, together
Reason is included within the scope of the present invention.
Claims (5)
1. a kind of low temperature abscisic acid, it is characterised in that by selected Xanthohumate, import fermentation chelatase, salicylic acid, Glucose
Freeze proof immune factor is according to 1：1.5-2：1.5-2：2.5-3 weight is made than well mixed.
2. the application method of the low temperature abscisic acid described in a kind of claim 1, it is characterised in that comprise the following steps：
A, getting fat strong sprout：Mid-October in autumn, an organic fertilizer is applied to fruit strain, then apply a phosphorus potash fertilizer, improve carbon-nitrogen rate；
B, ferment are stimulated：Entered before the winter, from late October, the useless bud flowers and fruits, red once by the strain of same seed pod were applied every 12-18 days
The environmental protection ferment being made after sugar, water by fermentation, by 1:1000 ratio adds water and is diluted rear drip irrigation, pours and apply or foliage dressing, even
It is continuous to use 3 stimulation plant hormones；
C, spray low temperature abscisic acid：Press 1 within 5-8 days before each cooling:600 ratio is diluted with water low temperature abscisic acid, then uniform spray
Plant is spilt, until there is droplet.
3. the application method of low temperature abscisic acid according to claim 2, it is characterised in that " the B, ferment are stimulated " step
In, the environmental protection ferment preparation method is：Petal shape fruit under being dredged using the tender shoots of same seed pod, flower and fruit thinning, insect pest cull fruit
With brown sugar, water according to 2-3:1:In the 8-10 weight sealing container more nonmetallic than mixing and putting into one, partial containers are reserved
Space, will open bung and deflate and stir for every 2 days the 1st month, and sealing container is statically placed in into shady place from 2-3 months produces.
4. the application method of low temperature abscisic acid according to claim 2, it is characterised in that low temperature abscisic acid dilutes the whole winter
It is applied 3-4 times, when temperature is reduced to 5 DEG C or less, it is impossible to spray.
5. the application method of low temperature abscisic acid according to claim 2, it is characterised in that in " A, getting fat strong sprout " step,
Carbon-nitrogen rate after improvement is 4：6.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107646479A (en) * | 2017-10-26 | 2018-02-02 | 刘窕敏 | A kind of propagation method of five happinesses persimmon seedling |
| CN110915353A (en) * | 2019-11-28 | 2020-03-27 | 天津大学 | Method for relieving stress injury of nano zinc oxide to plants by using salicylic acid |
| CN114853536A (en) * | 2022-06-02 | 2022-08-05 | 广西起凤橘洲生态农业有限公司 | Preparation method and application of agricultural enzyme |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1528147A (en) * | 2003-09-29 | 2004-09-15 | 广州大学 | Salicylic acid cold resistance agent and method for cold resistance |
| CN104585239A (en) * | 2015-02-02 | 2015-05-06 | 山西省农业科学院棉花研究所 | Multifunctional fruit tree low temperature-resistant preparation and preparation method thereof |
| CN105519581A (en) * | 2014-09-29 | 2016-04-27 | 赖婷婷 | Kiwifruit antifreeze agent |
-
2017
- 2017-04-27 CN CN201710290359.0A patent/CN107047617A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1528147A (en) * | 2003-09-29 | 2004-09-15 | 广州大学 | Salicylic acid cold resistance agent and method for cold resistance |
| CN105519581A (en) * | 2014-09-29 | 2016-04-27 | 赖婷婷 | Kiwifruit antifreeze agent |
| CN104585239A (en) * | 2015-02-02 | 2015-05-06 | 山西省农业科学院棉花研究所 | Multifunctional fruit tree low temperature-resistant preparation and preparation method thereof |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107646479A (en) * | 2017-10-26 | 2018-02-02 | 刘窕敏 | A kind of propagation method of five happinesses persimmon seedling |
| CN110915353A (en) * | 2019-11-28 | 2020-03-27 | 天津大学 | Method for relieving stress injury of nano zinc oxide to plants by using salicylic acid |
| CN114853536A (en) * | 2022-06-02 | 2022-08-05 | 广西起凤橘洲生态农业有限公司 | Preparation method and application of agricultural enzyme |
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Application publication date: 20170818 |