CN104206242A - Annual factory production process for grape virus-free tube seedlings - Google Patents
Annual factory production process for grape virus-free tube seedlings Download PDFInfo
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- CN104206242A CN104206242A CN201410470140.5A CN201410470140A CN104206242A CN 104206242 A CN104206242 A CN 104206242A CN 201410470140 A CN201410470140 A CN 201410470140A CN 104206242 A CN104206242 A CN 104206242A
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Abstract
The invention belongs to the technical field of agricultural planting, and particularly relates to an annual factory production process for grape virus-free tube seedlings. According to the original flow process, the transplanting can only be carried out in spring, the time is short, the equipment, tool and labor pressure can be caused by excessive concentrated transplanting, and the breeding speed and the benefits of the virus-free tube seedlings are influenced. In order to overcome the defect, under the intelligence-free greenhouse conditions, the technical flow process is improved, in a novel technical flow process, transplanting seasons are increased, the transplanting pressure is relieved, the annual production is realized, and the positive significance is realized on the grape virus-free tube seedling breeding acceleration.
Description
technical field:
The invention belongs to agriculture cultivation technology field, specifically belong to a kind of grape detoxification test tube plantlet anniversary factory production process.
background technology:
At present varied for the method for grape fast breeding, the grape seedlings of still breeding can only be transplanted within the short some months time in spring, and transplanting rear guarantee down will have three months growth times, just can produce finished product seedling sells, under the condition without intelligent greenhouse, cannot accomplish anniversary batch production production, like this in the time of commercial distribution, because customer demand is large, nurturing period is short, causes the source of goods in short supply, produces relatively concentrated, cost is high, and efficiency is also low.
summary of the invention:
Therefore, be necessary to invent a kind of grape detoxification test tube plantlet anniversary factory production process.
A kind of grape detoxification test tube plantlet anniversary factory production process, comprises following flow process:
(1) transplant spring:
Every year the 3-5 month carrying out transplanting in spring, step is as follows:
1) light training hardening: the grape vermiculite test-tube plantlet of having grown is unscrewed to bottle cap in light training chamber, under 0.4-2 ten thousand lx light, cultivate after 2-3 days, uncap is moisturizing 90% in seedling bed, after covering glass, humidity reduces by 10% from 90% every day, light intensity increases by 20% from 20,000 lx every day, light training hardening is after 5-8 days, and newborn cauline leaf becomes glossy, and cane is rubescent can transplant to seedling culture hole plate;
2) seedling culture hole plate hardening: after light training hardening, heat and moisture preserving moves between transplanting cart, the light carrying out again in seedling bed 3-5 days is trained after hardening, transplants to seedling culture hole plate moisturizing 90% in seedling bed, after covering glass, humidity reduces by 5% from 90% every day, light intensity increases by 20% from 10,000 lx every day, until be reduced to booth humidity and be increased to booth light intensity, seedling culture hole plate hardening is after 15-25 days, newborn cauline leaf doubles, and can transplant to nutritive cube;
3) nutritive cube hardening: after seedling culture hole plate hardening, transplant to nutritive cube, inside pack flower cultivating soil, vermiculite and perlite into, ratio is 3:1:1, starts cover glass moisturizing in 5-7 days, and glass is opened to entirely and takes off gradually afterwards, after 30-45 days, seedling length is twice, and through taking off the canopy high light 9-11 days that is exposed to the sun, can be used as commercial seedling and sells;
4) if do not sell as commercial seedling, cave can be coiled to seedling or nutrition pot seedling directly plants to sun light green house with the seeding row spacing of 20x25 centimetre, prophase management is avoided high light direct projection, humidity is controlled at 70%～80%, temperature is between 22～28 DEG C, after two weeks, increase gradually illumination, reduce humidity, allow transplanted seedling adapt to gradually environment in canopy and do not stop growth, transplant after one month, regularly spray nitrogen, phosphorus, potassium foliage fertilizer, be equipped with iron, zinc, boron, in whole vegetative period, execute altogether foliage fertilizer 6 times, the extermination of disease and insect pest 5 times, cultivates into superfine seedling and sells;
(2) retarding of growing in summer:
The 6-8 month, shoot survival percent was low because temperature is high, thus do not transplant, and test-tube grape seedling is inoculated in vermiculite medium, cultivate in air-conditioned culturing room, also can carry out shoot proliferation, after aestivating, carry out again transplanting in autumn to mid-August;
(3) transplant autumn:
Grape detoxification test tube plantlet is bred for the simple and easy vermiculite medium that places penicillin in propagation, summer through agar medium, autumn is again through light training hardening, seedling culture hole plate hardening, move in green house ground, finished product seedling is sold, the seedling that does not reach finished product standard continues to stay in booth cultivates and survives the winter, and next year cultivates into superfine seedling and sells.
The present invention has following technical advantage:
1. improved the fast numerous technological process of production of test-tube grape, realized and produced and transplant for many months.
2. under without intelligent greenhouse condition, the anniversary of accomplishing produces.
3. the simple and easy vermiculite Propagations of Teat Tube Seedlings of grape, just planting the first two months spring in the past carries out, because of sugar-free and organic principle, at most can only subculture once, can not long term subculture, now innovation, in the season that can not transplant, proceeds to agar seedling to add in the simple and easy vermiculite medium of penicillin test tube and goes to cultivate, and can continue non-stop expansion numerous.
4. vermiculite medium detoxification test tube plantlet is not transplanted in the time that summer weather is too warm, and utilizing has the culturing room of temperature equipment to carry out vermiculite medium to continue to cultivate the 2-3 month, transplants after spending summer high temperature season again.
5. vermiculite medium detoxic seedling test-tube plantlet, in the way of air-conditioned culturing room employing retarding of growing, promotes the aging maturation of test-tube plantlet, and next year transplants again, can tide over severe winter.
6. former technological process can not be transplanted autumn, and this technological process can be transplanted in the fall, and has high survival rate, and in booth, continue grow and tide over severe winter, exceed three months vegetative period, and twig can be all aging and ripe, safe overwintering, next year, most of can germination survived, and can produce superfine seedling.
7. this new technological flow increases considerably transplanting time, alleviate the pressure that equipment, apparatus and artificial undue concentration are transplanted, the anniversary of accomplishing produces and transplants, and has expanded breeding quantity and transplanting, reduce costs, to accelerating the production of grape detoxic seedling and applying significant.
brief description of the drawings:
Fig. 1 transplants light training hardening spring;
Fig. 2 is seedling culture hole plate hardening;
Fig. 3 is nutritive cube hardening;
Fig. 4 is cave dish seedling direct transplantation to growing state in sun light green house.
Fig. 5 transplants to growing state in sun light green house autumn;
Fig. 6 transplants to survive germination to spring next year in sun light green house autumn;
Fig. 7 be transplant autumn in sun light green house spring next year growing state.
embodiment:
A kind of grape detoxification test tube plantlet anniversary factory production process, comprises following flow process:
(1) transplant spring:
Every year the 3-5 month carrying out transplanting in spring, step is as follows:
1) light training hardening (as shown in Figure 1): the grape vermiculite test-tube plantlet of having grown is unscrewed to bottle cap in light training chamber, under 0.4-2 ten thousand lx light, cultivate after 2-3 days, uncap is moisturizing 90% in seedling bed, after covering glass, humidity reduces by 10% from 90% every day, light intensity increases by 20% from 20,000 lx every day, light training hardening is after 5-8 days, and newborn cauline leaf becomes glossy, and cane is rubescent can transplant to seedling culture hole plate;
2) seedling culture hole plate hardening (as shown in Figure 2): after light training hardening, heat and moisture preserving moves between transplanting cart, the light carrying out again in seedling bed 3-5 days is trained after hardening, transplants to seedling culture hole plate moisturizing 90% in seedling bed, after covering glass, humidity reduces by 5% from 90% every day, light intensity increases by 20% from 10,000 lx every day, until be reduced to booth humidity and be increased to booth light intensity, seedling culture hole plate hardening is after 15-25 days, newborn cauline leaf doubles, and can transplant to nutritive cube;
3) nutritive cube hardening (as shown in Figure 3): after seedling culture hole plate hardening, transplant to nutritive cube, inside pack flower cultivating soil, vermiculite and perlite into, ratio is 3:1:1, starts cover glass moisturizing in 5-7 days, and glass is opened to entirely and takes off gradually afterwards, after 30-45 days, seedling length is twice, and through taking off the canopy high light 9-11 days that is exposed to the sun, can be used as commercial seedling and sells;
4) (as shown in Figure 4) be not if sell as commercial seedling, cave can be coiled to seedling or nutrition pot seedling directly plants to sun light green house with the seeding row spacing of 20x25 centimetre, prophase management is avoided high light direct projection, humidity is controlled at 70%～80%, temperature is between 22～28 DEG C, after two weeks, increase gradually illumination, reduce humidity, allow transplanted seedling adapt to gradually environment in canopy and do not stop growth, transplant after one month, regularly spray nitrogen, phosphorus, potassium foliage fertilizer, be equipped with iron, zinc, boron, in whole vegetative period, execute altogether foliage fertilizer 6 times, the extermination of disease and insect pest 5 times, cultivates into superfine seedling and sells;
(2) retarding of growing in summer:
The 6-8 month, shoot survival percent was low because temperature is high, thus do not transplant, and test-tube grape seedling is inoculated in vermiculite medium, cultivate in air-conditioned culturing room, also can carry out shoot proliferation, after aestivating, carry out again transplanting in autumn to mid-August;
(3) transplant autumn:
Grape detoxification test tube plantlet is bred for the simple and easy vermiculite medium that places penicillin in propagation, summer through agar medium, autumn is again through light training hardening, seedling culture hole plate hardening, move in green house ground, finished product seedling is sold, the seedling that does not reach finished product standard continues to stay in booth cultivates and survives the winter, and next year cultivates into superfine seedling and sells.
Table 1: spring and summer transplanting survival rate
PINOT NOIR spring and summer is transplanted to monthly statistics and transplant number, the results are shown in Table 1, as known from Table 1, spring and summer difference month transplanting survival rate difference, 2 month temperatures are lower, unfavorable transplanting.May is the highest, is secondly April, March, enters 6 month temperature rising survival rates and reduces, drop to more than 43%, and excess Temperature thereafter, without intelligent greenhouse, temperature can not be fallen, and just stops transplanting, and therefore, from transplanting, preferably month is the 3-5 month.
Table 2: different grape variety transplanting survival rates in autumn and minor matters aging conditions
Table 3: different grape variety is transplanted next year survival rate autumn
Table 3 has been added up the Merlot of transplanting autumn, Cabernet Sauvignon, Cabernet Gernischt, Chardonnay, Gao Te, 1103 stocks and anti-seven grape varieties of three stocks and stock, 11069 strains before surviving the winter, 9058 strains after surviving the winter, overwintering survival rate 81.80%, although plant and cause seedling quality not reach out garden requirement late, but twig degree of aging is high, thereby overwintering survival rate is also high, shows that transplant this autumn is successful.
Table 4: the survival rate that lifting next year in autumn transplants
Table 4 shows: the vermiculite seedling that three of PINOT NOIRs strain is transplanted autumn, next year survival rate all high, Second Year takes 2947 young plants, separately plant in nutritive cube, survive 2144 strains after first quarter moon, survival rate reaches 72.75%, majority survives, thereby transplant in this kind of autumn can be for the production of.
From table 2-4, Fig. 5, Fig. 6 and Fig. 7, in temperature applicable autumn, through light training hardening → seedling culture hole plate hardening → be colonizated in into canopy, survival rate is still high, average survival 74.58%, grow also normal, after severe winter, in the nothing greenhouse of heating, twig is aging maturation also, and leading thread rugosity is 1.36-3.93mm, ripe aging full bud 1.5-3.92, all there is aging full bud, no matter strong sprout and weak seedling are absolutely aging, for next year sprouting lays the first stone.But this slow cultivation seedling, leading thread rugosity is less than 6mm, and ripe aging full bud less than 5 saves, and does not reach out garden requirement, can only cultivate again.Table 2 is also shown in simultaneously, and along with the postponement of shift time in autumn, twig also degree of aging and leading thread rugosity declines thereupon.
The whole year in 2013 is produced nursery stock 76719 strains altogether, transplants vermiculite medium seedling autumn and survives 21866 strains, and the seedling of whole year production seedling number nearly 1/3 is the increase that new technological flow is produced, and this is initial trial result, is bound to thereafter carry out a step raising.The another this seedling that does not reach out garden requirement, continuing this year to cultivate to become superfine seedling.Simultaneously also can lifting, separately plant in nutritive cube, for the PINOT NOIR seedling that the has order pot seedling that can partly supplement the nutrients again, for Field planting.It can be said that bright this new technological process, increase considerably transplanting time, alleviate the pressure that equipment, apparatus and artificial undue concentration are transplanted, be worth advocating and promoting.
Claims (1)
1. a grape detoxification test tube plantlet anniversary factory production process, is characterized in that: comprise following flow process:
(1) transplant spring:
Every year the 3-5 month carrying out transplanting in spring, step is as follows:
1) light training hardening: the grape vermiculite test-tube plantlet of having grown is unscrewed to bottle cap in light training chamber, under 0.4-2 ten thousand lx light, cultivate after 2-3 days, uncap is moisturizing 90% in seedling bed, after covering glass, humidity reduces by 10% from 90% every day, light intensity increases by 20% from 20,000 lx every day, light training hardening is after 5-8 days, and newborn cauline leaf becomes glossy, and cane is rubescent can transplant to seedling culture hole plate;
2) seedling culture hole plate hardening: after light training hardening, heat and moisture preserving moves between transplanting cart, the light carrying out again in seedling bed 3-5 days is trained after hardening, transplants to seedling culture hole plate moisturizing 90% in seedling bed, after covering glass, humidity reduces by 5% from 90% every day, light intensity increases by 20% from 10,000 lx every day, until be reduced to booth humidity and be increased to booth light intensity, seedling culture hole plate hardening is after 15-25 days, newborn cauline leaf doubles, and can transplant to nutritive cube;
3) nutritive cube hardening: after seedling culture hole plate hardening, transplant to nutritive cube, inside pack flower cultivating soil, vermiculite and perlite into, ratio is 3:1:1, starts cover glass moisturizing in 5-7 days, and glass is opened to entirely and takes off gradually afterwards, after 30-45 days, seedling length is twice, and through taking off the canopy high light 9-11 days that is exposed to the sun, can be used as commercial seedling and sells;
4) if do not sell as commercial seedling, cave can be coiled to seedling or nutrition pot seedling directly plants to sun light green house with the seeding row spacing of 20x25 centimetre, prophase management is avoided high light direct projection, humidity is controlled at 70%～80%, temperature is between 22～28 DEG C, after two weeks, increase gradually illumination, reduce humidity, allow transplanted seedling adapt to gradually environment in canopy and do not stop growth, transplant after one month, regularly spray nitrogen, phosphorus, potassium foliage fertilizer, be equipped with iron, zinc, boron, in whole vegetative period, execute altogether foliage fertilizer 6 times, the extermination of disease and insect pest 5 times, cultivates into superfine seedling and sells;
(2) retarding of growing in summer:
The 6-8 month, shoot survival percent was low because temperature is high, thus do not transplant, and test-tube grape seedling is inoculated in vermiculite medium, cultivate in air-conditioned culturing room, also can carry out shoot proliferation, after aestivating, carry out again transplanting in autumn to mid-August;
(3) transplant autumn:
Grape detoxification test tube plantlet is bred for the simple and easy vermiculite medium that places penicillin in propagation, summer through agar medium, autumn is again through light training hardening, seedling culture hole plate hardening, move in green house ground, finished product seedling is sold, the seedling that does not reach finished product standard continues to stay in booth cultivates and survives the winter, and next year cultivates into superfine seedling and sells.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410470140.5A CN104206242B (en) | 2014-09-16 | 2014-09-16 | A kind of Fructus Vitis viniferae detoxification test tube plantlet anniversary factory production process |
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| CN201410470140.5A CN104206242B (en) | 2014-09-16 | 2014-09-16 | A kind of Fructus Vitis viniferae detoxification test tube plantlet anniversary factory production process |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105393891A (en) * | 2015-11-26 | 2016-03-16 | 宁夏林业研究所股份有限公司 | Grape tissue culture seedling transplanting method for drought region |
| CN107333655A (en) * | 2017-08-17 | 2017-11-10 | 宁夏农垦西夏王实业有限公司葡萄苗木分公司 | A kind of micro- numerous screening and culturing medium of alkali resistance grape rootstock and micro- numerous screening technique |
| CN115053723A (en) * | 2017-03-07 | 2022-09-16 | 三菱化学水解决方案株式会社 | Seedling raising device and seedling raising method |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102293111A (en) * | 2011-06-24 | 2011-12-28 | 凉山州中泽新技术开发有限责任公司 | Method for raising seedlings by cutting olives |
-
2014
- 2014-09-16 CN CN201410470140.5A patent/CN104206242B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102293111A (en) * | 2011-06-24 | 2011-12-28 | 凉山州中泽新技术开发有限责任公司 | Method for raising seedlings by cutting olives |
Non-Patent Citations (5)
| Title |
|---|
| 孙腾飞，卢诚，于海森，张建军: "葡萄单芽育苗技术", 《中外葡萄与葡萄酒》 * |
| 曹孜义等: "葡萄试管快速繁殖工厂化生产", 《农业科技通讯》 * |
| 沈传进，王利民，张平，闫晕花，安旭军，李振德: "鲜食葡萄组织培养快速繁殖系统的建立", 《河北农业科学》 * |
| 裴宝霞: "葡萄试管苗移栽技术要点", 《河北农业科技》 * |
| 郝会军, 陈美霞, 巨荣峰, 房师: "半夏组培苗炼苗技术研究", 《安徽农业科学》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105393891A (en) * | 2015-11-26 | 2016-03-16 | 宁夏林业研究所股份有限公司 | Grape tissue culture seedling transplanting method for drought region |
| CN105393891B (en) * | 2015-11-26 | 2018-05-25 | 宁夏林业研究所股份有限公司 | A kind of Tissue culture the seedling of grape method for transplanting of suitable arid area |
| CN115053723A (en) * | 2017-03-07 | 2022-09-16 | 三菱化学水解决方案株式会社 | Seedling raising device and seedling raising method |
| CN107333655A (en) * | 2017-08-17 | 2017-11-10 | 宁夏农垦西夏王实业有限公司葡萄苗木分公司 | A kind of micro- numerous screening and culturing medium of alkali resistance grape rootstock and micro- numerous screening technique |
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Effective date of registration: 20200901 Address after: 750104 the Ningxia Hui Autonomous Region County, Yongning County, Yuquanying, Yinchuan Patentee after: Ningxia Yuquan nursery stock Co., Ltd Address before: 750001, Yongning County, the Ningxia Hui Autonomous Region, Yuquanying farm Yinchuan Yong Huang Road next to Patentee before: GRAPE NURSERY STOCK BRANCH OF NINGXIA NONGKEN XIXIAWANG INDUSTRIAL Co.,Ltd. |
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