CN104206242B - A kind of Fructus Vitis viniferae detoxification test tube plantlet anniversary factory production process - Google Patents

A kind of Fructus Vitis viniferae detoxification test tube plantlet anniversary factory production process Download PDF

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CN104206242B
CN104206242B CN201410470140.5A CN201410470140A CN104206242B CN 104206242 B CN104206242 B CN 104206242B CN 201410470140 A CN201410470140 A CN 201410470140A CN 104206242 B CN104206242 B CN 104206242B
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seedling
light
test tube
transplant
exercising
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CN104206242A (en
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曹孜义
何金柱
王宏
张新勤
任彦青
郎凤红
杨贵荷
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Ningxia Yuquan nursery stock Co., Ltd
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GRAPE NURSERY STOCK BRANCH OF NINGXIA NONGKEN XIXIAWANG INDUSTRIAL Co Ltd
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Abstract

The invention belongs to technical field of agricultural cultivation, particularly belong to a kind of Fructus Vitis viniferae detoxification test tube plantlet anniversary factory production process, former technological process, can only transplant in spring, time is short, undue concentration is transplanted, and causes equipment, apparatus and artificial pressure, has influence on detoxification test tube plantlet reproduction speed and benefit, in order to overcome this not enough, under the conditions of without intelligent greenhouse, we improve technological process, new technological process, add and transplant season, alleviate and transplant pressure, accomplish whole year production, have positive effect to accelerating the breeding of Fructus Vitis viniferae detoxification test tube plantlet.

Description

A kind of Fructus Vitis viniferae detoxification test tube plantlet anniversary factory production process
Technical field:
The invention belongs to technical field of agricultural cultivation, particularly belong to a kind of Fructus Vitis viniferae detoxification test tube plantlet week Year factory production process.
Background technology:
The most varied for the method for Fructus Vitis viniferae fast breeding, but the grape seedlings bred is only Can transplant within the short some months time in spring, ensure to have three months when growing after transplanting lower ground Between, just can produce finished product Seedling and sell, under conditions of without intelligent greenhouse, it is impossible to accomplish week Year factorial praluction, so when commercial distribution, owing to customer demand is big, the nurturing period is short, Causing the source of goods in short supply, produce Relatively centralized, cost is high, and efficiency is the lowest.
Summary of the invention:
Therefore, it is necessary to a kind of Fructus Vitis viniferae detoxification test tube plantlet anniversary factory production process of invention.
A kind of Fructus Vitis viniferae detoxification test tube plantlet anniversary factory production process, including below scheme:
(1) transplant spring:
Carrying out transplanting in spring in the annual 3-5 month, step is as follows:
1) light training seedling exercising: the Fructus Vitis viniferae Vermiculitum test tube Seedling grown is unscrewed bottle cap, at 0.4-2 in light training room After cultivating 2-3 days under ten thousand lx light, open lid moisturizing 90% in seedling bed, cover glass After glass, humidity reduces by 10% from 90% every day, and light intensity increases by 20% from 20,000 lx every day, light training refining After Seedling 5-8 days, newborn stem and leaf becomes glossy, and cane is rubescent can transplant to seedling culture hole plate;
2) seedling culture hole plate seedling exercising: after light training seedling exercising, heat and moisture preserving moves into transplants workshop, at seedling bed After carrying out the light training seedling exercising of 3-5 days the most again, transplant to seedling culture hole plate, moisturizing in seedling bed 90%, after covering glass, humidity reduces by 5% from 90% every day, and light intensity increases from 10,000 lx every day 20%, until being reduced to booth humidity and increasing to booth light intensity, seedling culture hole plate seedling exercising 15-25 After it, newborn stem and leaf doubles, and can transplant to nutritive cube;
3) nutritive cube seedling exercising: after seedling culture hole plate seedling exercising, transplants to nutritive cube, interior loading flower cultivating soil, Vermiculitum and perlite, ratio is 3:1:1, starts 5-7 days cover glass moisturizing, afterwards glass Being gradually opened and entirely take off, after 30-45 days, Seedling length is twice, and is exposed to the sun 9-11 through taking off canopy high light My god, can be used as commercial seedling and sell;
4) if selling not as commercial seedling, can be by Plug seedling or nutrition pot seedling with the strain of 20x25 centimetre Line-spacing is directly planted to sun light green house, and prophase management avoids high light direct projection, humid control 70%~ 80%, temperature, between 22~28 DEG C, is gradually increased illumination after two weeks, reduces humidity, allows shifting Plant Seedling gradually to adapt to environment in canopy and do not stop growth, after transplanting one month, periodically spray nitrogen, phosphorus, Potassium foliage fertilizer, is equipped with ferrum, zinc, boron, executes foliage fertilizer altogether 6 times, pest and disease damage in whole trophophase Prevent and treat 5 times, be trained superfine Seedling and sell;
(2) retarding of growing in summer:
The 6-8 month is high due to temperature, and shoot survival percent is low, so not transplanting, and by Fructus Vitis viniferae test tube Seedling It is inoculated in Vermiculitum culture medium, cultivates in air-conditioned culturing room, also can carry out subculture multiplication, Carry out transplanting in autumn again to mid-August after aestivating;
(3) transplant autumn:
Fructus Vitis viniferae detoxification test tube plantlet is through agar culture medium subculture multiplication, the letter that places penicillin summer Easily Vermiculitum culture medium breeding, trains seedling exercising, seedling culture hole plate seedling exercising through light again, moves into greenhouse big autumn In canopy ground, finished product Seedling is sold, and does not reaches the Seedling of finished product standard and continues to stay to cultivate in booth and survive the winter, Next year is trained superfine Seedling and sells.
The present invention has a following technical advantage:
1. improve the Fructus Vitis viniferae tube rapid propagation technological process of production, it is achieved that within many months, produce and transplant.
2., under the conditions of without intelligent greenhouse, whole year production can be accomplished.
3. Fructus Vitis viniferae simple Vermiculitum Propagations of Teat Tube Seedlings, the most simply plants the first two months and carry out, because of nothing spring Sugar and organic principle, at most can only subculture once, it is impossible to long term subculture, existing innovation for In the season that can not transplant, agar Seedling is proceeded to add in penicillin simple Vermiculitum culture medium test tube Cultivate, non-stop expanding propagation can be continued.
4. Vermiculitum culture medium detoxification test tube plantlet is not transplanted when summer weather is the warmmest, utilizes and has homoiothermic The culturing room of equipment carries out Vermiculitum culture medium and persistently cultivates the 2-3 month, after spending summer high temperature season Transplant again.
5. Vermiculitum culture medium detoxic seedling test tube Seedling, uses retarding of growing in air-conditioned culturing room Way, promote the aging maturation of test tube Seedling, next year transplants, can tide over severe winter.
The most former technological process can not be transplanted autumn, and this technological process can be transplanted in the fall, and has High survival rate, and continued propagation tiding over severe winter in booth, trophophase more than three months, Twig can be the most aging and ripe, safe overwintering, and next year, most of can germination survived, and can produce Superfine Seedling.
The most this new technological flow increases considerably transplanting time, alleviates equipment, apparatus and people The pressure that work undue concentration is transplanted, accomplishes whole year production and transplanting, expands reproductive number and shifting Plant, reduce cost, to accelerating the production of Fructus Vitis viniferae detoxic seedling and applying significant.
Accompanying drawing illustrates:
Fig. 1 is to transplant light training seedling exercising spring;
Fig. 2 is seedling culture hole plate seedling exercising;
Fig. 3 is nutritive cube seedling exercising;
Fig. 4 is that hole tray seedling direct transplantation is to growing state in sun light green house.
Fig. 5 is to transplant to growing state in sun light green house autumn;
Fig. 6 is to transplant autumn to survive germination to spring next year in sun light green house;
Fig. 7 is to transplant to growing state in spring next year in sun light green house autumn.
Detailed description of the invention:
A kind of Fructus Vitis viniferae detoxification test tube plantlet anniversary factory production process, including below scheme:
(1) transplant spring:
Carrying out transplanting in spring in the annual 3-5 month, step is as follows:
1) light training seedling exercising (as shown in Figure 1): the Fructus Vitis viniferae Vermiculitum test tube Seedling grown is unscrewed in light training room Bottle cap, after cultivating 2-3 days, opens lid and protects in seedling bed under 0.4-2 ten thousand lx light Wet 90%, after covering glass, humidity reduces by 10% from 90% every day, and light intensity increases from 20,000 lx every day Adding 20%, light training seedling exercising is after 5-8 days, and newborn stem and leaf becomes glossy, and cane is rubescent can transplant To seedling culture hole plate;
2) seedling culture hole plate seedling exercising (as shown in Figure 2): after light training seedling exercising, heat and moisture preserving moves into transplanting cart Between, after carrying out the light training seedling exercising of 3-5 days in seedling bed again, transplant to seedling culture hole plate, educating Moisturizing 90% in seedbed, after covering glass, humidity reduces by 5% from 90% every day, and light intensity is from 10,000 lx Every day increases by 20%, until being reduced to booth humidity and increasing to booth light intensity, seedling culture hole plate refines After Seedling 15-25 days, newborn stem and leaf doubles, and can transplant to nutritive cube;
3) nutritive cube seedling exercising (as shown in Figure 3): after seedling culture hole plate seedling exercising, transplant to nutritive cube, interior Loading flower cultivating soil, Vermiculitum and perlite, ratio is 3:1:1, starts 5-7 days cover glasses and protects Wet, glass is gradually opened and entirely takes off afterwards, and after 30-45 days, Seedling length is twice, strong through taking off canopy Light is exposed to the sun 9-11 days, can be used as commercial seedling and sells;
4) (as shown in Figure 4) if selling not as commercial seedling, can by Plug seedling or nutrition pot seedling with The seeding row spacing of 20x25 centimetre is directly planted to sun light green house, and prophase management avoids high light direct projection, Humid control is 70%~80%, and temperature, between 22~28 DEG C, is gradually increased illumination after two weeks, Reducing humidity, in allowing transplanted seedling gradually adapt to canopy, environment does not stop growth, after transplanting one month, Periodically spray nitrogen, phosphorus, potassium foliage fertilizer, be equipped with ferrum, zinc, boron, in whole trophophase, execute blade face altogether Fertile 6 times, the prevention and control of plant diseases, pest control 5 times, it is trained superfine Seedling and sells;
(2) retarding of growing in summer:
The 6-8 month is high due to temperature, and shoot survival percent is low, so not transplanting, and by Fructus Vitis viniferae test tube Seedling It is inoculated in Vermiculitum culture medium, cultivates in air-conditioned culturing room, also can carry out subculture multiplication, Carry out transplanting in autumn again to mid-August after aestivating;
(3) transplant autumn:
Fructus Vitis viniferae detoxification test tube plantlet is through agar culture medium subculture multiplication, the letter that places penicillin summer Easily Vermiculitum culture medium breeding, trains seedling exercising, seedling culture hole plate seedling exercising through light again, moves into greenhouse big autumn In canopy ground, finished product Seedling is sold, and does not reaches the Seedling of finished product standard and continues to stay to cultivate in booth and survive the winter, Next year is trained superfine Seedling and sells.
Table 1: spring and transplanting survival rate in summer
PINOT NOIR spring and summer is transplanted monthly statistics and transplants number, the results are shown in Table 1, as known from Table 1, Spring and summer difference month, transplanting survival rate was different, and 2 month temperatures are relatively low, unfavorable transplanting.May The highest, next to that April, March, enter 6 month temperatures and rise high-survival rate reduction, under Reducing to more than 43%, temperature is too high thereafter, and without intelligent greenhouse, temperature fall is not got off, and just stops Only transplanting, therefore from the point of view of transplanting, preferably month is the 3-5 month.
Table 2: different grape variety transplanting survival rates in autumn and minor matters aging conditions
Table 3: different grape varieties transplant next year survival rate autumn
Table 3 added up autumn transplant Merlot, Cabernet Sauvignon, Cabernet Gernischt, Chardonnay, Gao Te, 1103 stocks and anti-seven grape varieties of three stocks and stock, 11069 strain before surviving the winter, survive the winter Rear 9058 strains, overwintering survival rate 81.80%, although plant late and cause seedling quality not reach out garden Requirement, but twig degree of aging is high, thus overwintering survival rate is the highest, shows that this autumn transplants It is successful.
Table 4: the survival rate that lifting next year in autumn transplants
Table 4 shows: the Vermiculitum Seedling of PINOT NOIR three strains transplanting in autumn, and next year survival rate is the highest, Second Year takes 2947 young plants, separately plants in nutritive cube, survives 2144 strains, survive after first quarter moon Rate reaches 72.75%, and majority survives, thus transplants and may be used for producing this kind of autumn.
From upper table 2-4, Fig. 5, Fig. 6 and Fig. 7, in the autumn that temperature is suitable for, through light Training seedling exercising → seedling culture hole plate seedling exercising → be colonizated in into canopy, survival rate is the highest, average survival 74. 58%, growth is also normal, after severe winter, in the greenhouse without heating, and the most aging maturation of twig, Footpath, ground rugosity is 1.36-3.93mm, ripe aging full bud 1.5-3.92, all with always Change full bud, no matter strong sprout and weak Seedling are the most aging, sprout for next year and lay the first stone.But This slow cultivation Seedling, footpath, ground rugosity is less than 6mm, and ripe aging full bud saves less than 5, does not reaches Go out garden requirement, can only be further cultured for.Table 2 is also shown in simultaneously, along with the postponement of shift time in autumn, Twig also degree of aging and footpath, ground rugosity decline therewith.
The whole year in 2013 produces nursery stock 76719 strain altogether, transplants Vermiculitum culture medium Seedling autumn 21866 strains alive, the Seedling of whole year production Seedling number nearly 1/3 is the increase that new technological flow produces, This is initial trial result, is bound to thereafter carry out a step raising.The most this garden that do not reaches out is wanted The Seedling asked, continues cultivation this year and can become superfine Seedling.The most also can lifting, separately plant into nutritive cube In, for there being the PINOT NOIR Seedling of order can partly supplement the nutrients again pot seedling, for Field planting. It can be said that bright this new technological process, increase considerably transplanting time, alleviate equipment, The pressure that apparatus and artificial undue concentration are transplanted, is worth advocating and promoting.

Claims (1)

1. a Fructus Vitis viniferae detoxification test tube plantlet anniversary factory production process, it is characterised in that: include with Lower flow process:
(1) transplant spring:
Carrying out transplanting in spring in the annual 3-5 month, step is as follows:
1) light training seedling exercising: the Fructus Vitis viniferae Vermiculitum test tube Seedling grown is unscrewed bottle cap, at 0.4-2 in light training room After cultivating 2-3 days under ten thousand lx light, open lid moisturizing 90% in seedling bed, cover glass After glass, humidity reduces by 10% from 90% every day, and light intensity increases by 20% from 20,000 lx every day, light training refining After Seedling 5-8 days, newborn stem and leaf becomes glossy, and cane is rubescent can transplant to seedling culture hole plate;
2) seedling culture hole plate seedling exercising: after light training seedling exercising, heat and moisture preserving moves into transplants workshop, at seedling bed After carrying out the light training seedling exercising of 3-5 days the most again, transplant to seedling culture hole plate, moisturizing in seedling bed 90%, after covering glass, humidity reduces by 5% from 90% every day, and light intensity increases from 10,000 lx every day 20%, until being reduced to booth humidity and increasing to booth light intensity, seedling culture hole plate seedling exercising 15-25 After it, newborn stem and leaf doubles, and can transplant to nutritive cube;
3) nutritive cube seedling exercising: after seedling culture hole plate seedling exercising, transplants to nutritive cube, interior loading flower cultivating soil, Vermiculitum and perlite, ratio is 3:1:1, starts 5-7 days cover glass moisturizing, afterwards glass Being gradually opened and entirely take off, after 30-45 days, Seedling length is twice, and is exposed to the sun 9-11 through taking off canopy high light My god, can be used as commercial seedling and sell;
4) if selling not as commercial seedling, by Plug seedling or nutrition pot seedling with the plant of 20x25 centimetre Away from directly planting in sun light green house, prophase management avoids high light direct projection, humid control 70%~ 80%, temperature, between 22~28 DEG C, is gradually increased illumination after two weeks, reduces humidity, allows shifting Plant Seedling gradually to adapt to environment in canopy and do not stop growth, after transplanting one month, periodically spray nitrogen, phosphorus, Potassium foliage fertilizer, is equipped with ferrum, zinc, boron, executes foliage fertilizer altogether 6 times, pest and disease damage in whole trophophase Prevent and treat 5 times, be trained superfine Seedling and sell;
(2) retarding of growing in summer:
The 6-8 month is high due to temperature, and shoot survival percent is low, so not transplanting, and by Fructus Vitis viniferae test tube Seedling It is inoculated in Vermiculitum culture medium, cultivates in air-conditioned culturing room, or carry out subculture multiplication, degree Carry out transplanting in autumn again to mid-August after summer;
(3) transplant autumn:
Fructus Vitis viniferae detoxification test tube plantlet is through agar culture medium subculture multiplication, the letter that places penicillin summer Easily Vermiculitum culture medium breeding, trains seedling exercising, seedling culture hole plate seedling exercising through light again, moves into greenhouse big autumn In canopy ground, finished product Seedling is sold, and does not reaches the Seedling of finished product standard and continues to stay to cultivate in booth and survive the winter, Next year is trained superfine Seedling and sells.
CN201410470140.5A 2014-09-16 2014-09-16 A kind of Fructus Vitis viniferae detoxification test tube plantlet anniversary factory production process Active CN104206242B (en)

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CN105393891B (en) * 2015-11-26 2018-05-25 宁夏林业研究所股份有限公司 A kind of Tissue culture the seedling of grape method for transplanting of suitable arid area
WO2018163629A1 (en) * 2017-03-07 2018-09-13 三菱ケミカルアグリドリーム株式会社 Rice seedling cultivation device and rice seedling cultivation method
CN107333655A (en) * 2017-08-17 2017-11-10 宁夏农垦西夏王实业有限公司葡萄苗木分公司 A kind of micro- numerous screening and culturing medium of alkali resistance grape rootstock and micro- numerous screening technique

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Effective date of registration: 20200901

Address after: 750104 the Ningxia Hui Autonomous Region County, Yongning County, Yuquanying, Yinchuan

Patentee after: Ningxia Yuquan nursery stock Co., Ltd

Address before: 750001, Yongning County, the Ningxia Hui Autonomous Region, Yuquanying farm Yinchuan Yong Huang Road next to

Patentee before: GRAPE NURSERY STOCK BRANCH OF NINGXIA NONGKEN XIXIAWANG INDUSTRIAL Co.,Ltd.