CN102712912A - 在乳酸菌中改善流向乙酰乳酸来源产物的流量 - Google Patents

在乳酸菌中改善流向乙酰乳酸来源产物的流量 Download PDF

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CN102712912A
CN102712912A CN2010800539407A CN201080053940A CN102712912A CN 102712912 A CN102712912 A CN 102712912A CN 2010800539407 A CN2010800539407 A CN 2010800539407A CN 201080053940 A CN201080053940 A CN 201080053940A CN 102712912 A CN102712912 A CN 102712912A
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gene
cell
lactic
seq
sequence
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B·J·保罗
W·苏
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Butamax Advanced Biofuels LLC
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    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/16—Butanols
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/746—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for lactic acid bacteria (Streptococcus; Lactococcus; Lactobacillus; Pediococcus; Enterococcus; Leuconostoc; Propionibacterium; Bifidobacterium; Sporolactobacillus)
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01027—L-Lactate dehydrogenase (1.1.1.27)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C12Y203/01054—Formate C-acetyltransferase (2.3.1.54), i.e. pyruvate formate-lyase or PFL
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y401/00—Carbon-carbon lyases (4.1)
    • C12Y401/01—Carboxy-lyases (4.1.1)
    • C12Y401/01005—Acetolactate decarboxylase (4.1.1.5)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
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  • Molecular Biology (AREA)
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  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
CN2010800539407A 2009-09-29 2010-09-29 在乳酸菌中改善流向乙酰乳酸来源产物的流量 Pending CN102712912A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US24671709P 2009-09-29 2009-09-29
US61/246717 2009-09-29
PCT/US2010/050705 WO2011041402A1 (en) 2009-09-29 2010-09-29 Improved flux to acetolactate-derived products in lactic acid bacteria

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US (1) US9169499B2 (US07125668-20061024-C00013.png)
EP (1) EP2483400A1 (US07125668-20061024-C00013.png)
JP (1) JP2013505739A (US07125668-20061024-C00013.png)
CN (1) CN102712912A (US07125668-20061024-C00013.png)
AU (1) AU2010300722A1 (US07125668-20061024-C00013.png)
BR (1) BR112012006941A2 (US07125668-20061024-C00013.png)
CA (1) CA2776151A1 (US07125668-20061024-C00013.png)
IN (1) IN2012DN02396A (US07125668-20061024-C00013.png)
WO (1) WO2011041402A1 (US07125668-20061024-C00013.png)

Cited By (9)

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CN107922917A (zh) * 2015-06-12 2018-04-17 Cj第制糖株式会社 乳酸生产率提高的微生物和使用其生产乳酸的方法
CN108368523A (zh) * 2015-10-09 2018-08-03 丹麦技术大学 在代谢工程化的乳酸菌中高水平生产双乙酰
CN108486176A (zh) * 2018-05-18 2018-09-04 天津科技大学 一种产乳酸乙酯的酿酒酵母及其构建方法与应用
CN108570438A (zh) * 2017-03-08 2018-09-25 中国科学院上海高等研究院 提升克雷伯氏肺炎杆菌生产α-酮异戊酸的方法及改造菌
CN110431229A (zh) * 2016-12-14 2019-11-08 瓦赫宁根大学 热稳定的Cas9核酸酶
CN111893083A (zh) * 2019-05-05 2020-11-06 中国科学院上海高等研究院 改造的克雷伯氏属细菌及其生产2,3-二羟基异戊酸的应用
CN112601809A (zh) * 2018-08-03 2021-04-02 米兰-比科卡大学 细胞生物合成聚d-乳酸和聚l-乳酸的方法
CN113473998A (zh) * 2019-02-01 2021-10-01 农业食品和环境国家研究中心 用于预防和/或治疗内脏痛的乳酸乳球菌菌株
CN114457122A (zh) * 2020-05-13 2022-05-10 安徽华恒生物科技股份有限公司 生产l-缬氨酸的重组微生物及构建方法、应用

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US8188250B2 (en) 2008-04-28 2012-05-29 Butamax(Tm) Advanced Biofuels Llc Butanol dehydrogenase enzyme from the bacterium Achromobacter xylosoxidans
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BR112012006939A2 (pt) * 2009-09-29 2015-09-08 Butamax Tm Advanced Biofuels célula hospedeira de produção de levedura recombinante e método para a produção de um produto selecionado do grupo que consiste em 2,3-butanodiol, isobutanol, 2-butanol, 1-butanol, 2-butanona, valina, leucina, ácido lático, ácido málico, álcool, isoampilico e isoprenoides
WO2011082248A1 (en) 2009-12-29 2011-07-07 Butamax(Tm) Advanced Biofuels Llc Expression of hexose kinase in recombinant host cells
KR20120115349A (ko) 2009-12-29 2012-10-17 부타맥스 어드밴스드 바이오퓨얼스 엘엘씨 저급 알킬 알코올의 발효 생성에 유용한 알코올 탈수소효소(ａｄｈ)
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JP2014519811A (ja) 2011-04-29 2014-08-21 ダニスコ・ユーエス・インク チオラーゼ、ＨＭＧ−ＣｏＡ合成酵素及びＨＭＧ−ＣｏＡ還元酵素活性を有するポリペプチドをコードしている遺伝子を使用するメバロン酸、イソプレン、及びイソプレノイドの生産
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JP2015510774A (ja) 2012-03-23 2015-04-13 ビュータマックス・アドバンスド・バイオフューエルズ・エルエルシー ブタノール産生菌用培地の酢酸補給
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CN107922917A (zh) * 2015-06-12 2018-04-17 Cj第制糖株式会社 乳酸生产率提高的微生物和使用其生产乳酸的方法
CN108368523A (zh) * 2015-10-09 2018-08-03 丹麦技术大学 在代谢工程化的乳酸菌中高水平生产双乙酰
CN110431229B (zh) * 2016-12-14 2024-03-12 瓦赫宁根大学 热稳定的Cas9核酸酶
CN110431229A (zh) * 2016-12-14 2019-11-08 瓦赫宁根大学 热稳定的Cas9核酸酶
US12071639B2 (en) 2016-12-14 2024-08-27 Wageningen Universiteit Thermostable Cas9 nucleases
US11976306B2 (en) 2016-12-14 2024-05-07 Stichting Voor De Technische Wetenschappen Thermostable CAS9 nucleases
US11939605B2 (en) 2016-12-14 2024-03-26 Wageningen Universiteit Thermostable CAS9 nucleases
CN108570438A (zh) * 2017-03-08 2018-09-25 中国科学院上海高等研究院 提升克雷伯氏肺炎杆菌生产α-酮异戊酸的方法及改造菌
CN108486176A (zh) * 2018-05-18 2018-09-04 天津科技大学 一种产乳酸乙酯的酿酒酵母及其构建方法与应用
CN108486176B (zh) * 2018-05-18 2021-08-03 天津科技大学 一种产乳酸乙酯的酿酒酵母及其构建方法与应用
CN112601809A (zh) * 2018-08-03 2021-04-02 米兰-比科卡大学 细胞生物合成聚d-乳酸和聚l-乳酸的方法
CN113473998A (zh) * 2019-02-01 2021-10-01 农业食品和环境国家研究中心 用于预防和/或治疗内脏痛的乳酸乳球菌菌株
CN111893083A (zh) * 2019-05-05 2020-11-06 中国科学院上海高等研究院 改造的克雷伯氏属细菌及其生产2,3-二羟基异戊酸的应用
CN114457122B (zh) * 2020-05-13 2023-06-20 安徽华恒生物科技股份有限公司 生产l-缬氨酸的重组微生物及构建方法、应用
CN114457122A (zh) * 2020-05-13 2022-05-10 安徽华恒生物科技股份有限公司 生产l-缬氨酸的重组微生物及构建方法、应用

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