CN102657060A - Transplantation and cultivation method for tissue-culture bottle seedlings of betula alnoides - Google Patents
Transplantation and cultivation method for tissue-culture bottle seedlings of betula alnoides Download PDFInfo
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- CN102657060A CN102657060A CN2012101506882A CN201210150688A CN102657060A CN 102657060 A CN102657060 A CN 102657060A CN 2012101506882 A CN2012101506882 A CN 2012101506882A CN 201210150688 A CN201210150688 A CN 201210150688A CN 102657060 A CN102657060 A CN 102657060A
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Abstract
The invention discloses a transplantation and cultivation method for tissue-culture bottle seedlings of betula alnoides. The transplantation and cultivation method comprises the steps of: exercising the tissue-culture bottle seedlings of the betula alnoides, preparing substrates, processing the substrates before transplantation, transplanting, and cultivating the seedlings of the betula alnoides in greenhouse seedling nurseries, wherein a seedling container is made of degradable non-woven fabrics, the transmittance of a shading net ranges from 40% to 50%, the seedling container is arranged in a seedling disc, holes are formed in the bottom surface of the seedling disc, the distance from the seedling disc to the ground is more than 15cm, the temperature is controlled between 20 to 26 DEG C, the relative air humidity ranges from 75% to 90%, the water content of the substrate ranges from 60% to 70%, and multiple-chromobacterium-prodigiosum wettable powder can be used for atomizing; after nursery-grown plants survive for 28 to 32 days, the transmittance of the shading net is controlled to 60% to 70%, and the relative air humidity is controlled to 60% to 70%; and after the nursery-grown plants survive for 50 to 60 days, the shading net is uncovered. According to the method disclosed by the invention, the bottle seedlings only need to be cultivated for 90 to 100 days to obtain finished seedlings, the survival rate is more than 91%, and the survival rate of the seedlings is improved by 1.94 times compared with that in the prior art.
Description
Technical field
The present invention relates to forest seed cultivation technical field, be specifically related to the breeding method of Birch finished product seedling.
Background technology
Birch (
Betula alnoides) Betulaceae, Betula; Belong to speed and give birth to deciduous species; China mainly is distributed in western part, the northwestward, the west and south and southern areas, Guizhou Province and Vietnam, Laos and the Burma of hot-zone, Yunnan Province, Guangxi province; Birch since its timber be difficult for warping and fracturing, not yielding, decorative pattern and color and luster are attractive in appearance to have extensive use at traditional top-grade furniture, timber floor, house decorative material, building, military project, musical instrument, plywood etc.Hot-zone, Yunnan Province area accounts for 1/4th of the whole province's area, and Birch becomes the precious speed in hot-zone, Yunnan Province and gives birth to deciduous species, also is main reproducting tree species.
Developing Clonal Forestry is a kind of advanced method that pierre technology such as the group training, grafting, cuttage through defect individual is cultivated the afforestation that good asexual nursery stock uses, it have the purpose product yield high, manage the advantage that intensity is high, operating cycle is short, product quality is neat and good in economic efficiency.Recent two decades comes; Developing Clonal Forestry becomes the common the most popular brainstorm subject of forestry of most of in the world developed countries and many developing countries; At present; Technology such as Birch asexual propagation, seed selection are day by day ripe, but also there are some problems in the transplant of the tissue-culture container seedling that matches with Birch pierre technology.From the Birch tissue-culture container seedling to become can go up a hill afforestation the finished product seedling; The existing polythene bagged plant of adopting more; Whole seedling raising process is not had meticulous administrative skill measure,, make that the survival rate of cultivating into the finished product seedling is very low easily because of water stain Birch nursery stock stem rot, the root-rot etc. of causing.
Summary of the invention
The technical problem that the present invention will solve is to overcome the low shortcoming of existing Birch tissue-culture container seedling transplant survival rate and not
Foot its objective is the transplant method that the high Birch tissue-culture container seedling of a kind of cultivating survival rate is provided.
For solving the problems of the technologies described above, technical scheme of the present invention is following:
A kind of transplant method of Birch tissue-culture container seedling, carry out according to the following steps:
(1) the refining seedling of Birch tissue-culture container seedling
When treating that the Birch tissue-culture container seedling grows to 2～3 merogenesis, the Birch tissue culture bottle taken out of from culturing room to place temperature be under 20～27 ℃ the natural daylight 10-15 days;
(2) preparation of seedling medium
Selecting peat and red heart soil is the preparation of raw material seedling medium, and red heart soil is apart from the laterite below the 50cm of laterite surface; Volume ratio by peat and red heart soil is: peat is 60-70%, and red heart soil promptly is made into described seedling medium for the ratio of 30-40% with native mixing of peat and red heart;
(3) selection of container for plant growth
The selection diameter is 6～8cm, and height is the degradable nonwoven container for plant growth of 11～13cm;
(4) processing of matrix before the transplanting
With step (2) the matrix prepared described container for plant growth of step (3) of packing into, water permeable after, be made into 1000～1500 times of liquid with 50% carbendazol wettable powder and water the matrix of drenching in the container for plant growth in second day;
(5) transplant
The described Birch tissue-culture container seedling of step (1) is taken out from bottle, is 2～3cm with root pruning, and 800～1000 times of liquid putting into 50% carbendazol wettable powder again dip in the matrix that is planted in the described container for plant growth of step (4) behind the root;
(6) cultivation of Birch nursery stock in the booth nursery
1. take light transmittance at top, booth nursery and be 40%～50% shading screen; The container for plant growth of step (5) having been planted the Birch tissue cultivating seedling is placed in the seedling-cultivating tray; The bottom surface of seedling-cultivating tray is provided with porose, and the aperture is placed on seedling-cultivating tray in the booth nursery less than the diameter of this container for plant growth; With the yoke plate rail height of will growing seedlings, the bottom surface that makes seedling-cultivating tray is apart from more than the ground 15cm in the booth nursery;
2. canopy temperature is controlled at 20～26 ℃; The spraying of Birch nursery stock is watered; Control air relative moisture is 75%～90%, and the mass water content of matrix is 60%～70%, transplants in back 7 days every day with 1000～1500 times of liquid sprayings of more than 50% clever bacterium wetting powders or water and drench the Birch nursery stock 1 time; Later on whenever, sprayed with 1000～1500 times of liquid of more than 50% clever bacterium wetting powders at a distance from 3～7 days or water pouring Birch nursery stock 1 time; Treat that the Birch nursery stock becomes to live back 28 days～32 days, the light transmittance of control shading screen is 60%～70%, and control air relative moisture is 60～70%; Treat that the Birch nursery stock becomes back alive 50-60 days, throws off shading screen fully;
(7) outplanting
Height of seedling reaches 20～25cm can go out the garden.
Compared with prior art, the invention has the beneficial effects as follows:
1, the inventive method is according to the physiological development characteristic of Birch seeds; Adopt aggregate measures such as the Birch tissue cultivating seedling carries out the cultivation of finished product seedling, suitable matrix preparation in the cultivating process of Birch finished product seedling, sterilization, illumination, air humidity, water management can reach about 20cm the Birch tissue cultivating seedling being cultivated into height of seedling in short 90-110 days; The taper seedling hat that forms multi-branched is the finished product seedling that can go out the garden; Can directly afforest; To the survival rate of cultivating the finished product seedling that can directly be used to afforest more than 91%, shortened 10～15 day than the growing-seedling period of existing polythene bagged plant from the Birch tissue cultivating seedling, survival rate has improved 194%.
2, the Birch finished product seedling seedling root that adopts the inventive method to breed is complete, and balance stretches, and helps forming main root and avoids twining root, helps to form sturdy, a large amount of root systems.
3, adopt the inventive method to transplant the Birch tissue cultivating seedling, can reduce the generation of soil-borne disease effectively, the generation of stem rot, root-rot significantly is lower than existing polythene bagged plant.
Embodiment
Below among each embodiment material such as used degradable nonwoven container for plant growth, 50% carbendazol wettable powder, Birch tissue cultivating seedling all can buy in market.The time of Birch tissue cultivating seedling transplant is got final product according to the cultivation of 3 months in advance local afforestation time in rainy season.There is not the conventional method that is that specifies among the embodiment.
Implement the place: the Puer City, Yunnan Province
Embodiment 1 the inventive method embodiment
Carry out (3 repetitions) according to the following steps:
(1) the refining seedling of Birch tissue-culture container seedling
When treating that the Birch tissue-culture container seedling grows to 2～3 merogenesis, Birch tissue cultivating seedling bottle taken out of from culturing room to place temperature be 20 ℃～23 ℃ following 10 days of natural daylight;
(2) preparation of seedling medium
Selecting peat and red heart soil is the preparation of raw material seedling medium, and described red heart soil is apart from the laterite below the 50cm of laterite surface; Volume ratio by peat and red heart soil is: peat is 60%, and to be 40% ratio with peat and red heart soil be mixed red heart soil promptly is made into described seedling medium;
(3) selection of container for plant growth
The selection diameter is 6cm, and height is the degradable nonwoven container for plant growth of 11cm;
(4) processing of matrix before the transplanting
With step (2) the matrix prepared described container for plant growth of step (3) of packing into, water permeable after, be made into 1000 times of liquid with 50% carbendazol wettable powder and water the matrix of drenching in the container for plant growth in second day;
(5) transplant
The described Birch tissue-culture container seedling through the refining seedling of step (1) is taken out from bottle, is 2～3cm with root pruning, and 800 times of liquid putting into 50% carbendazol wettable powder again dip in the matrix that is planted in the described container for plant growth of step (4) behind the root;
(6) cultivation of Birch seedling in the booth nursery
1. take light transmittance at top, booth nursery and be 40% shading screen, place the frame of growing seedlings in the booth nursery
,The container for plant growth of step (5) having been planted the Birch tissue cultivating seedling is placed in the seedling-cultivating tray; The bottom surface of described seedling-cultivating tray is provided with porose; The aperture is placed on seedling-cultivating tray in the booth nursery less than the diameter of this container for plant growth, with the yoke plate rail height of will growing seedlings; The outer bottom that makes seedling-cultivating tray except that with the yoke contact portion, remaining outer bottom part of seedling-cultivating tray only contacts with air; The bottom surface of seedling-cultivating tray is apart from more than the ground 15cm in the booth nursery;
2. canopy temperature is controlled at 20～23 ℃; The spraying of Birch nursery stock is watered, and control air relative moisture remains on 75%～80%, and the mass water content of matrix is 60%; Transplant in back 7 days every day with 1000 times of liquid sprayings of more than 50% clever bacterium wetting powders Birch nursery stock 1 time; Later on every at a distance from 3 days with 1000 times of liquid sprayings of more than 50% clever bacterium wetting powders Birch nursery stock 1 time, treated Birch nursery stock one-tenth back 28 days alive, throw off the part shading screen; The light transmittance of control shading screen is 60%, and control air relative moisture is 60%～65%; Treat that the Birch nursery stock becomes to live back 50 days, throws off shading screen fully;
(7) outplanting
Height of seedling reaches 20～25cm, and the taper seedling hat that forms multi-branched can go out the garden becomes the finished product seedling, the Birch nursery stock is connected whole degradable nonwoven container for plant growth directly be planted in field planting.
Present embodiment sees table 1 from the Birch tissue-culture container seedling for details to the transplant survival rate of cultivating into the finished product seedling.
Embodiment 2
Embodiment 2 is except that following measure difference, and all the other measures are identical with embodiment 1, no longer repeat.
(1) the refining seedling of Birch tissue-culture container seedling
Birch tissue cultivating seedling bottle taken out of from culturing room to place temperature be 25～27 ℃ following 15 days of natural daylight;
(2) preparation of seedling medium
Volume ratio by peat and red heart soil is: peat is 70%, and to be 30% ratio with peat and red heart soil be mixed red heart soil promptly is made into described seedling medium;
(3) selection of container for plant growth
The selection diameter is 8cm, and height is the degradable nonwoven container for plant growth of 13cm;
(4) processing of matrix before the transplanting
Be made into 1500 times of liquid with 50% carbendazol wettable powder in second day and water the matrix of drenching in the container for plant growth;
(5) transplant
50% carbendazol wettable powder that the Birch tissue cultivating seedling dips in root is 1000 times of liquid;
(6) cultivation of Birch nursery stock in the booth nursery
1. take light transmittance at top, booth nursery and be 50% shading screen;
2. canopy temperature is controlled at 24～26 ℃, and the spraying of Birch nursery stock is watered, and control air relative moisture remains on 80～90%; The mass water content of matrix is 70%; Transplant in back 7 days to water with 1500 times of liquid of more than 50% clever bacterium wetting powders every day and drench the Birch nursery stock 1 time, every later on watering with 1500 times of liquid of more than 50% clever bacterium wetting powders at a distance from 7 days drenched the Birch nursery stock 1 time, treats nursery stock one-tenth back 32 days alive; The light transmittance of control shading screen is 70%, and control air relative moisture is 65%～70%; Treat that nursery stock becomes to live back 60 days, throws off shading screen fully;
Present embodiment sees table 1 from the Birch tissue cultivating seedling for details to the transplant survival rate of cultivating into the finished product seedling.
Contrast (CK) is to adopt conventional seedbed system, in little shed, and the 100% forest table soil of packing in educating with polythene bagged plant, plastic sack, water 1 time/day, one deck light transmittance of manual work is 55% shading screen, becomes to live back about 40 days the removal shading screen.
The transplant survival rate application form of finished product seedling is bred in table 1 embodiment 1-embodiment 2 and contrast
Claims (1)
1. the transplant method of a Birch tissue-culture container seedling, carry out according to the following steps:
(1) the refining seedling of Birch tissue-culture container seedling
When treating that the Birch tissue-culture container seedling grows to 2～3 merogenesis, the Birch tissue culture bottle taken out of from culturing room to place temperature be under 20～27 ℃ the natural daylight 10-15 days;
(2) preparation of seedling medium
Selecting peat and red heart soil is the preparation of raw material seedling medium, and red heart soil is apart from the laterite below the 50cm of laterite surface; Volume ratio by peat and red heart soil is: peat is 60-70%, and red heart soil promptly is made into described seedling medium for the ratio of 30-40% with native mixing of peat and red heart;
(3) selection of container for plant growth
The selection diameter is 6～8cm, and height is the degradable nonwoven container for plant growth of 11～13cm;
(4) processing of matrix before the transplanting
With step (2) the matrix prepared described container for plant growth of step (3) of packing into, water permeable after, be made into 1000～1500 times of liquid with 50% carbendazol wettable powder and water the matrix of drenching in the container for plant growth in second day;
(5) transplant
The described Birch tissue-culture container seedling of step (1) is taken out from bottle, is 2～3cm with root pruning, and 800～1000 times of liquid putting into 50% carbendazol wettable powder again dip in the matrix that is planted in the described container for plant growth of step (4) behind the root;
(6) cultivation of Birch nursery stock in the booth nursery
1. in the booth nursery inner top to take light transmittance be 40%～50% shading screen; The described container for plant growth of step (5) is placed in the seedling-cultivating tray; The bottom surface of described seedling-cultivating tray is provided with porose, and the aperture is placed on seedling-cultivating tray in the booth nursery less than the diameter of this container for plant growth; With the yoke plate rail height of will growing seedlings, make the seedling-cultivating tray bottom surface apart from more than the 15cm of ground;
2. canopy temperature is controlled at 20～26 ℃; The spraying of Birch nursery stock is watered; Control air relative moisture is 75%～90%, and the mass water content of matrix is 60%～70%, transplants in back 7 days every day with 1000～1500 times of liquid sprayings of more than 50% clever bacterium wetting powders or water and drench the Birch nursery stock 1 time; Later on whenever, sprayed with 1000～1500 times of liquid of more than 50% clever bacterium wetting powders at a distance from 3～7 days or water pouring Birch nursery stock 1 time; Treat that the Birch nursery stock becomes to live back 28 days～32 days, the light transmittance of control shading screen is 60%～70%, and control air relative moisture is 60～70%; Treat that the Birch nursery stock becomes back alive 50～60 days, throws off shading screen fully;
(7) outplanting
When reaching 20～25cm, height of seedling goes out the garden.
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| CN2012101506882A CN102657060B (en) | 2012-05-16 | 2012-05-16 | Transplantation and cultivation method for tissue-culture bottle seedlings of betula alnoides |
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| CN2012101506882A CN102657060B (en) | 2012-05-16 | 2012-05-16 | Transplantation and cultivation method for tissue-culture bottle seedlings of betula alnoides |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102934572A (en) * | 2012-10-11 | 2013-02-20 | 中国林业科学研究院热带林业研究所 | Method for cultivating seedlings of betula alnoides in shallow water |
| CN103039240A (en) * | 2012-12-28 | 2013-04-17 | 重庆新开源农业开发有限公司 | Seeding and seedling raising method for waterbirch in Three Gorges Reservoir region |
| CN104012367A (en) * | 2014-06-23 | 2014-09-03 | 云南省林业技术推广总站 | Afforestation method of betula alnoides clone tissue culture seedlings |
| CN104641918A (en) * | 2015-03-16 | 2015-05-27 | 黄振忠 | Seedling growing method for betula alnoides |
| CN105532398A (en) * | 2015-12-18 | 2016-05-04 | 成都天开锦城植物科技有限公司 | Light-matrix degradable-nonwoven-fabric ex-vitro-rooting seedling raising method for Hosta plantaginea tissue culture seedlings |
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| CN101796922A (en) * | 2009-02-11 | 2010-08-11 | 中国林业科学研究院热带林业实验中心 | Betula alnoides leaf bud tissue culture rapid propagation technique |
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- 2012-05-16 CN CN2012101506882A patent/CN102657060B/en not_active Expired - Fee Related
Patent Citations (1)
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| CN101796922A (en) * | 2009-02-11 | 2010-08-11 | 中国林业科学研究院热带林业实验中心 | Betula alnoides leaf bud tissue culture rapid propagation technique |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102934572A (en) * | 2012-10-11 | 2013-02-20 | 中国林业科学研究院热带林业研究所 | Method for cultivating seedlings of betula alnoides in shallow water |
| CN102934572B (en) * | 2012-10-11 | 2014-04-23 | 中国林业科学研究院热带林业研究所 | Method for cultivating seedlings of betula alnoides in shallow water |
| CN103039240A (en) * | 2012-12-28 | 2013-04-17 | 重庆新开源农业开发有限公司 | Seeding and seedling raising method for waterbirch in Three Gorges Reservoir region |
| CN103039240B (en) * | 2012-12-28 | 2014-11-05 | 重庆新开源农业开发有限公司 | Seeding and seedling raising method for waterbirch in Three Gorges Reservoir region |
| CN104012367A (en) * | 2014-06-23 | 2014-09-03 | 云南省林业技术推广总站 | Afforestation method of betula alnoides clone tissue culture seedlings |
| CN104012367B (en) * | 2014-06-23 | 2016-03-30 | 云南省林业技术推广总站 | A kind of method of forestation of Birch clonal tissue culture seedling |
| CN104641918A (en) * | 2015-03-16 | 2015-05-27 | 黄振忠 | Seedling growing method for betula alnoides |
| CN105532398A (en) * | 2015-12-18 | 2016-05-04 | 成都天开锦城植物科技有限公司 | Light-matrix degradable-nonwoven-fabric ex-vitro-rooting seedling raising method for Hosta plantaginea tissue culture seedlings |
| CN105532398B (en) * | 2015-12-18 | 2018-07-27 | 四川农业大学 | A kind of hosta tissue culture seedling Light media degradable nonwoven fabrics outside sprout-cultivating-bottle seedling method |
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Granted publication date: 20130327 Termination date: 20190516 |