CN102599374A - Selenium-enriched kelp feed and application thereof to culture of selenium-enriched abalone - Google Patents
Selenium-enriched kelp feed and application thereof to culture of selenium-enriched abalone Download PDFInfo
- Publication number
- CN102599374A CN102599374A CN2012100789564A CN201210078956A CN102599374A CN 102599374 A CN102599374 A CN 102599374A CN 2012100789564 A CN2012100789564 A CN 2012100789564A CN 201210078956 A CN201210078956 A CN 201210078956A CN 102599374 A CN102599374 A CN 102599374A
- Authority
- CN
- China
- Prior art keywords
- selenium
- sea
- rich
- tangle
- abalone
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Landscapes
- Fodder In General (AREA)
- Feed For Specific Animals (AREA)
Abstract
The invention discloses a method for using nano-selenium contained kelp feed to culture selenium-enriched abalone, which includes: adding 440-1485ml of water to evenly dilute 0.50mg/ml red nano-selenium solution with particle size of 100-140nm, spraying the mixed liquor to kelp feed, and culturing for 120-180 days, wherein 0.50-2.0mg of nano-selenium is added per kilogram of the kelp feed, and shell length, weight, SOD (superoxide dismutase), GPX (glutathione peroxidase), survival rate and weight gain rate of the commercial selenium-enriched abalone are all increased evidently as compared with those of a control group. In addition, by comparative test for parameters such as nano-selenium dosage, diluted concentration, shape of kelp feed, culture duration and the like, a best method for culturing the commercial selenium-enriched abalone is further determined. The method is also applicable to production of selenium-enriched sea cucumber, fishes and shrimps.
Description
Technical field
The invention belongs to the nutrition immune field that learns a skill, relate to the application of nanometer microelement in aquaculture production, be specifically related to a kind of method that contains the sea-tangle feed of nanometer selenium and utilize its breeding selenium rich abalone
Background technology
Selenium is trace element necessary in human and animal's life, aspect the guarantee health multiple critical function being arranged, comprises immunological regulation, antifatigue, anti-oxidant, anti-aging.Immunity and anti-oxidation function lowly are the major reasons of multiple chronic disease (hypertension, coronary heart disease, diabetes, cancer).Science is mended selenium and is helped preventing the generation of above-mentioned disease, also can help human body opposing acute infectious disease, for example influenza.Bring into play these effects of selenium element, " the Chinese residents diet nutritional is with reference to the amount of taking the photograph " pointed out: the intake of selenium should be higher than 50 micrograms for each person every day.But Diet cures more than the doctors, and people should take in selenium from food chain.
As everyone knows, China has a soil to lack the selenium area from northeast to southwest, and China lacks selenium big country, and the area of area 72% is in low selenium area, and wherein 30% is the serious selenium area that lacks, and food selenium contents such as grain and vegetables are extremely low.The big and medium-sized cities of northeast, North China, northwest, southwest, south China, East China, the Delta of the Pearl River, Yangtze River Delta etc. all belong to low selenium area.At present; Still do not reach the above minimum selenium absorption level of 40 micrograms for each person every day in the population of China food, especially lack the more difficult purpose of mending selenium that from food, reaches of selenium area crowd, 28 micrograms are only arranged every day to 40 micrograms; Directly influence lacks selenium area resident's the general level of the health (Fu Shanlai, 2009).
Domestic animals and fowls lack selenium and are prone to suffer from the high white muscle disease of the death rate, have been the standard measure of animal husbandry so feed adds sodium selenite raising survival rate.The selenium element is by existing form can be divided into inorganic selenium (sodium selenite, sodium selenate), organic selenium (selenomethionine, selenium yeast) and zeroth order elemental selenium.Known grey and black elements selenium do not have biologically active.If the particle of zeroth order elemental selenium is a nanoscale, then take on a red color, be called red nano selenium.It is to thermally-stabilised, and is soluble in water, the characteristics that biologically active is high, acute toxicity is low.Its acute toxicity is 1/7 of an inorganic selenium, organic selenium 1/3, biological safety is high.Nanometer selenium also has stronger anti-oxidation function, higher bioavailability.Red nano selenium as feed addictive produce Se-enriched egg, to the existing reports such as influence (Wang Fuxiang etc., 2008, Wang Liang etc., 2011) of growth of meat chicken and oxidation resistance.The patent of invention ZL200810011963.6 of Tian Fuping contains nanometer selenium, zinc oxide organic biological feedstuff additive, method for making and method for using; With nanometer selenium, zinc oxide organic biological feedstuff additive; Produce selenium-rich milk, goat milk and egg, improve the immunity of domestic animals and fowls simultaneously.
Disease is that the important abalone culture kind of obstruction culture fishery development key problem, especially China---the sustainable development of haliotis discus hannai Ino receives serious harm, causes bigger economic loss always.The disease problem is from cause of disease, host and environment three factors.So doing well under the condition of breeding environment, the autoimmunity that improves abalone is the important factor of resist the disease.Selenium is the essential nutrient element of aquatic livestock, lacks or the excessive resistance and the neurological susceptibility that all can change animal to disease.Selenium is not directly to play a role as a kind of anti-oxidant, but as glutathione peroxidase (GPX) core part, and (Fang etc. 2002 to participate in the reset procedure of organic and inorganic peroxide; ).The shortage of selenium suppresses the aquatic animal growth, is the requisite nutritional trace element of animal.Excessive selenium has toxic action again in the feed on the other hand; Can cause that poor growth, the death rate increase etc., therefore need best selenium throwing amount, could keep antioxidant enzyme such as catalase (CAT) in the abalone body; Superoxide dismutase (SOD) (Zou Guolin etc.; 1986), glutathione peroxidase (GPX) (Bell etc., 1985) etc., eliminate the free radical (H that metabolism is produced
2O
2, O
2 -, MDA etc.), thereby effectively resist oxidational losses.Demand for selenium in the aquatic animal feed has had many research reports, like fish.And shell-fish also has some reports (Wang etc., 1994).For mollusk such as abalone Chinese Marine University Master's thesis (ten thousand is quick, 2003) report is arranged once.Above in a word report all is to use inorganic selenium---sodium selenite is the selenium source nutrient, does not see as yet with nanometer selenium and researchs and produces rich selenium abalone as the sea-tangle feed addictive and to the influence of anti-oxidant immunity.The mensuration of selenium is according to the method for GB5009.93-2010.
Summary of the invention
The purpose of this invention is to provide a kind of method that contains the sea-tangle feed breeding selenium rich abalone of nanometer selenium.It is little to make full use of nano granules of selenium, and penetration power is big, and abalone absorbs, and utilization rate is high, and toxicity is low, the wide advantage of safe dosage scope.Rich selenium abalone has good immunity, resistance to oxidation, is embodied as the motility rate original text, and raising the output is high, and selenium content is high.
Mentioned " red liquid nanometer selenium " is meant the nanometer selenium product of purchasing in Chemical Engineering College, Dalian Univ. of Science and Engineering (phone 0411-83020358) among the present invention in full; Its preparation method is; According among the patent of invention ZL200810011963.6, the method for " nanometer selenium organic-biological intermediate " among the embodiment 1 preparation is (that is, according in the former license text; Proportioning raw materials in Instructions Page 9 [0053] the row institute table, the method preparation of [0056]); The particle diameter of this product is 100～140nm, is mixed with concentration before using and is the concentration of the nanometer selenium liquid as 0.50mg/mL.
In order to solve the problems of the technologies described above, technical scheme of the present invention is:
One side of the present invention is; Disclose the Herba Zosterae marinae rich in selenium feed of a kind of breeding selenium rich abalone, it prepares through following steps: to particle diameter is that 100～140nm, concentration are in the red nano selenium solution of 0.50mg/mL, adds the water of 7～100 times of volumes; Make the nanometer selenium dilution; Again in nanometer selenium: bright sea-tangle is the ratio of 0.50～2.00mg: 1kg, whole dilutions evenly is sprayed on the bright sea-tangle of strip or pulp-like, processes after sea-tangle is dried.
Wherein, spray before to the red liquid nanometer selenium in different ratio thin ups; Can control the adhesive force of nanometer selenium through the difference of diluted concentration, thereby cause influence the rich selenium abalone feeding effect of feeding on the sea-tangle surface.That is: the higher relatively group of diluted concentration, it is not enough to be sprayed at the sea-tangle surface evenness, and feeding effect is not best; The group that diluted concentration is less relatively, nanometer selenium is little in sea-tangle adsorption power, and nanometer selenium is lost to the water from the sea-tangle surface easily in dropping into the breed pond, is unfavorable for abalone picked-up nanometer selenium, and feeding effect is not best.Therefore, even under sea-tangle and the situation of nanometer selenium at same ratio, because the diluted concentration of dilution nanometer selenium is different before spraying, the difference of the abalone effect that also can cause feeding.Therefore, spray the key that preceding diluted concentration difference of diluting nanometer selenium also is a preparation Herba Zosterae marinae rich in selenium feed.Though the method is simple and easy to do remarkably productive, not record in the prior art so far.
Herba Zosterae marinae rich in selenium feed mentioned above, under the optimal conditions, the nanometer selenium dilution is 1: 17～67 to make by the volume ratio of red nano selenium solution and water.
Herba Zosterae marinae rich in selenium feed mentioned above, under the optimal conditions, the nanometer selenium dilution is 1: 30～35 to make by the volume ratio of red nano selenium solution and water.
Herba Zosterae marinae rich in selenium feed mentioned above, under the optimal conditions, the nanometer selenium dilution is to make at 30: 970 by the volume ratio of red nano selenium solution and water.
Herba Zosterae marinae rich in selenium feed mentioned above, under the optimal conditions, in nanometer selenium: bright sea-tangle is that the ratio of 0.80～1.20mg: 1kg is sprayed onto the nanometer selenium dilution on the bright sea-tangle.
The throwing selenium amount of feeding sea-ear; Nanometer selenium just: the ratio of bright sea-tangle, along with its ratio increases, selenium content increases thereupon in the rich selenium abalone body; SOD vigor and GPX vigor not necessarily are linear enhancing in the body; Phenomenons such as poisoning or retarding of growing also can take place when excessive in the nutritious effect of nanometer selenium though this is, so nanometer selenium: the ratio of bright sea-tangle also is the key of preparation Herba Zosterae marinae rich in selenium feed.
Herba Zosterae marinae rich in selenium feed mentioned above, under the optimal conditions, in nanometer selenium: bright sea-tangle is that the ratio of 1.00mg: 1kg is sprayed onto the nanometer selenium dilution on the bright sea-tangle.
Another aspect of the present invention is, the method for a kind of breeding selenium rich abalone is provided, the Herba Zosterae marinae rich in selenium feed feeding sea-ear that it is mentioned above.
The method of breeding selenium rich abalone mentioned above, under the optimal conditions, its concrete method for breeding comprises:
1. get concentration 0.50mg/mL particle diameter 100～140nm red nano selenium solution 30.0mL, add water 970mL dilution evenly after, all evenly be sprayed at the surfaces of the bright sea-tangle of 15kg strip or slurry respectively, sea-tangle obtains Herba Zosterae marinae rich in selenium after placing and drying in 1-2 days;
The processing sea-tangle is 5～10cm * 5～10cm bar shaped, is the strip sea-tangle;
Be broken into less than 0.5cm with the hock type pulverizer
2The sea-tangle sheet is the slurry sea-tangle;
2. utilize the Herba Zosterae marinae rich in selenium feeding sea-ear 120～180 days that 1. step obtain, the raising condition is:
Indoor cement is cultured in the pond, and Chi Dipu goes into to culture the Bao lamina affixad, adds 3000/15m of abalone
2, piling the clean sea water after deposition, filtration treatment in the pond, seawater salt content 30 ‰, pH are 7.8～8.2; Dissolved oxygen 6～8mg/L; Water temperature remains on 10-24 ℃ in breeding process, the feed of throwing something and feeding during 16-17 every day; Inventory is 10% of an abalone TBW, and change breeding seawater next day during 9-11, washes away pool wall.
The method of the rich selenium abalone of above-mentioned raising that reaches described herein also can be widely used in aspects such as producing rich selenium sea cucumber, rich selenium fish or rich selenium shrimp.
Those skilled in the art can be through combining prior art or common practise; In the bait of sea cucumber, fish, shrimp etc., add nanometer selenium 1.00-1.50mg/kg; After drying,, feed sea cucumber, fish, shrimp etc. by different rate of charges; Promptly get the rich selenium sea cucumber of selenium content 0.50-1.00mg/kg, rich selenium fish and rich selenium shrimp, or the like.Owing to improved anti-oxidant immunity, reduce the incidence of disease, so can reduce or the use amount of added with antibiotic medicine not, also improve 5-15% output simultaneously, in concrete production application, its economic benefit increases significantly.
The invention has the beneficial effects as follows:
(1) at nanometer selenium dosage 0.50-2.00mg/kg; The rich selenium commodity abalone selenium content of strip and slurry sea-tangle is 0.205-0.706mg/kg and 0.282-0.781mg/kg respectively; And the commodity abalone selenium content of control group strip and slurry sea-tangle is respectively 0.132-0.166mg/kg and 0.175-0.202mg/kg; Rich selenium commodity abalone selenium content is 1.6-5.8 a times and 1.6-4.6 times of control group commodity abalone; Belong to rich selenium abalone, and meet the limit the quantity of requirement of sanitary standard (GB13105-91) Se content≤1.00mg/kg of selenium in the state food, the commodity abalone of safe selenium-complement is provided for people.
(2) SOD vigor in its body of breeding selenium rich commodity abalone process, the GPX vigor constantly increases, and is respectively 2.1 times and 1.9 times, 1.4 times and 1.3 times of control group (strip and slurry sea-tangle), the anti-oxidant immunity raising of self is described, the survival rate rising.Rich selenium strip sea-tangle survival rate rises to 91.3% from 85.0%, and rich selenium slurry sea-tangle survival rate rises to 92.7% from 88.5%, and rate of body weight gain rises to 19.6% and 21.53% respectively, is 1.3 times and 1.4 times of control group, and its economic benefit is appreciable.
(3) rich selenium commodity abalone breeding method is easy, has operability, saves energy consumption, raw material, operation, and economic effect is considerable.
(4) nanometer selenium is safe to use, and nanometer selenium sea-tangle processing, operation, control selenium concentration, method for using are simple and convenient; The feeding sea-ear absorbability is good, does not have problem of environmental pollution.
(5) also can be used for the production of rich selenium sea cucumber, precious fishes and shrimps etc.
(6) rich selenium abalone is not through the disease-resistant medicine of adding in the breeding environment, but through improving abalone self resistance, survival rate is beneficial to food security, the control of environmental pollution or radical cure.
The specific embodiment
Following non-limiting example can make those of ordinary skill in the art more fully understand the present invention, but does not limit the present invention in any way.
Mentioned " red liquid nanometer selenium " is meant the nanometer selenium product of purchasing in Chemical Engineering College, Dalian Univ. of Science and Engineering (phone 0411-83020358) in the embodiment of the invention; Its preparation method is; According among the patent of invention ZL200810011963.6, the method for " nanometer selenium organic-biological intermediate " among the embodiment 1 preparation is (that is, according in the former license text; Proportioning raw materials in Instructions Page 9 [0053] the row institute table, the method preparation of [0056]); The particle diameter of this product is 100～140nm, is mixed with concentration before using and is the concentration of the nanometer selenium liquid as 0.50mg/mL.
The SOD vigour-testing method is accomplished according to the method in the following list of references: Zou Guolin etc. the measuring method for activity of a kind of SOD.Biochemistry and biophysics progress, 1986,4:71-73.
The GPX vigour-testing method is accomplished according to the method in the following list of references: Bell; J.G.et, 1985.Some effects of vitamin E and selenium deprivation on tissue enzyme levels and indices of tissue peroxidation in rainbow trout (Salmon gairdneri) .Br.J.Nutr.53:149-157.
The mensuration of selenium content is according to the method for GB5009.93-2010.
The breeding environment of abalone described in the following embodiment is all carried out by following requirement:
Breeding environment: with the semi-finished product abalone (haliotis discus hannai Ino) of autotrophy, the long 6～7cm of average shell about heavy 50g, is placed in the cement pit in Dalian Shan Shui marine products Co., Ltd indoor culture base in the Lvshunkou District North Sea, Daliang City street pond floor space 15m
2, every pond Di Pu goes into to culture the Bao lamina affixad.Add 3000 of above-mentioned semi-finished product abalones, about 150kg, every like this pond is one group, is divided into 3 lattice.Pile the clean sea water after deposition, filtration treatment in the pond, salt content 30 ‰, pH are that 7.8～8.2. water temperature in breeding process remains on 10-24 ℃, dissolved oxygen 6～8mg/L.The feed of throwing something and feeding during 16-17 every day.Inventory is 10% of an abalone TBW, and promptly every lattice 4-5kg can adjust according to the situation of ingesting.Change breeding seawater next day during 9-11, washes away pool wall.Culturing fate is 120-180 days.
In identical breeding environment, with different forage feed abalones, feed divides following several kinds:
(1) control group feed:
Bright sea-tangle strip feed: bright sea-tangle, bar shaped, 5～10cm * 5-10cm; Be called the strip sea-tangle; That is, Control is 1 group.
Bright sea-tangle slurry feed: get bright sea-tangle, be broken into less than 0.5cm with the hock type pulverizer
2The sea-tangle sheet; Be called the slurry sea-tangle; That is, Control is 2 groups.
(2) experimental group feed:
Red liquid nanometer selenium after will diluting according to the ratio of " nanometer selenium: sea-tangle=0.50～2.00mg: 1kg " is sprayed at bright sea-tangle (being divided into strip sea-tangle, slurry sea-tangle) surface; Place and dried in 1-2 days; Promptly make the sea-tangle feed that contains the various dose nanometer selenium, subsequent use.
Before the sprinkling, to the red liquid nanometer selenium in a plurality of different ratio thin ups; Its objective is and grope the influence of diluted concentration the rich selenium abalone various features index of feeding, that is: the higher relatively group of diluted concentration, it is inhomogeneous to be sprayed at the sea-tangle surface, and local concentration is too high; The group that diluted concentration is less relatively, nanometer selenium is little in sea-tangle adsorption power, and nanometer selenium is discharged into the water from the sea-tangle surface easily in dropping into the breed pond, is unfavorable for abalone picked-up nanometer selenium, so the various features value is on the low side.Therefore, even under the certain situation of sea-tangle and nanometer selenium ratio, because the diluted concentration of dilution nanometer selenium is different before spraying, the difference of the abalone effect that also can cause feeding.Below in conjunction with specific embodiment the present invention is further specified:
Following table is among each embodiment, contains the preparation method of the dilution process and the Herba Zosterae marinae rich in selenium feed of various dose nanometer selenium:
Embodiment 1
Get red liquid nanometer selenium 15.0mL (calculating can know that containing the nanometer selenium amount is 7.5mg), each three parts.Add water 485,985 and 1485mL dilution respectively, mix, the nanometer selenium concentration in these three kinds of nanometer selenium dilutions is respectively 0.015mg/mL, 0.0075mg/mL, 0.0050mg/mL.Take by weighing three parts of 15kg strip sea-tangles in addition, respectively three kinds of dilutions all evenly are sprayed on the strip sea-tangle with vaporific, place and to dry 1-2 days, just make three kinds of (selenium: the rich selenium strip sea-tangle feed of sea-tangle=0.50mg/kg).According to above-mentioned breeding environment, it is added respectively in culturing the pond through 120 days breed, the result sees table 1.
It is as shown in the table: when thin up amount 985mL (group a-2); Rich selenium commodity abalone selenium content is 0.224mg/kg to the maximum; SOD vigor GPX vigor in the body is the highest; Reflect that anti-oxidant immunity is strong, the therefore average long 7.3cm of shell, body weight 80.5g, survival rate 90.5% and rate of body weight gain 16.1% are than other two groups all high.This is that it is inhomogeneous to be sprayed at the sea-tangle surface because of the little group 485mL of thin up amount (group a-1), and local concentration is too high; The group 1485mL that the thin up amount is big (group a-3), nanometer selenium is little in sea-tangle adsorption power, and nanometer selenium was discharged into the water from the sea-tangle surface easily after input was cultured the pond, was unfavorable for abalone picked-up nanometer selenium, so the various features value is on the low side.
Table 1. amount of water is to 0.50mg/kg
The strip sea-tangleCulture 120 days rich selenium commodity abalone influence
Embodiment 2
Respectively get three parts of 30.0mL red liquid nanometer selenium (calculating can know that containing the nanometer selenium amount is 15mg).Add water 470,970 and 1470mL dilution respectively; Mix; Nanometer selenium concentration in these three kinds of nanometer selenium dilutions is respectively 0.030mg/mL; 0.015mg/mL, the solution of 0.010mg/mL, other are operated with embodiment 1, just make three kinds of (selenium: the rich selenium strip sea-tangle feed of sea-tangle=1.00mg/kg).Add in culturing the pond through 120 days breed, the result sees table 2.
It is as shown in the table: when thin up amount 970mL (group b-1), rich selenium commodity abalone selenium content is 0.365mg/kg to the maximum, and SOD vigor and GPX vigor in the body are the highest, reflect that anti-oxidant immunity is strong, and all the other various features values also are the highest, than other 3 groups all high.
Selenium content, survival rate and rate of body weight gain than embodiment 1 (group a-2) have increased by 63%, 0.8% and 6.8% respectively.Comparison has increased by 1.8 times, 14.7% and 7.3% respectively according to group.Therefore best amount of water is 970mL (group b-2).
Table 2. amount of water is to 1.00mg/kg
The strip sea-tangleCulture 120 days rich selenium commodity abalone influence
Embodiment 3
Respectively get three parts of 45.0mL red liquid nanometer selenium.Add water 455,955 and 1455mL dilution respectively; Mix; Nanometer selenium concentration in these three kinds of nanometer selenium dilutions is respectively 0.045mg/mL; 0.0225mg/mL, the solution of 0.015mg/mL, other are operated with embodiment 1, just make three kinds of (selenium: the rich selenium strip sea-tangle feed of sea-tangle=1.50mg/kg).Add in culturing the pond through 120 days breed, the result sees table 3.
It is as shown in the table: when thin up amount 955mL (group c-2), rich selenium commodity abalone selenium content is 0.510mg/kg to the maximum, and selenium content increases by 39.7% than embodiment 2 (group b-2), but survival rate and rate of body weight gain reduce than embodiment 2 slightly.Though explain that throwing the selenium amount increases, the abalone selenium content increases thereupon, SOD vigor and GPX vigor no longer strengthen in the body.Though this is because the nutritious effect of nanometer selenium, also intoxicating phenomenon can take place when excessive, like retarding of growing etc.Therefore 955mL (group c-2) is best amount of water.
Table 3. amount of water is to 1.50mg/kg
The strip sea-tangleCulture 120 days rich selenium commodity abalone influence
Embodiment 4
Respectively get three parts of 60.0mL red liquid nanometer selenium; Add water 440,940 and 1440mL dilution respectively; Mix, the nanometer selenium concentration in these three kinds of nanometer selenium dilutions is respectively 0.060mg/mL, the solution of 0.030mg/mL, 0.020mg/mL; Other operations just make three kinds of (selenium: the rich selenium strip sea-tangle feed of sea-tangle=2.00mg/kg) with embodiment 1.Add in culturing the pond through 120 days breed, the result sees table 4.
It is as shown in the table: when thin up amount 940mL (group d-2), rich selenium commodity abalone selenium content is 0.706mg/kg to the maximum, and this is because the maximum reason of nanometer selenium dosage.And other various features values are all low than embodiment 2 and embodiment 3, throw selenium amount excess, and survival rate and rate of body weight gain begin to descend, and the trend of potentiality selenosis is arranged, unsuitable breeding selenium rich commodity abalone.
Table 4. amount of water is to 2.00mg/kg
The strip sea-tangleCulture 120 days rich selenium commodity abalone influence
Embodiment 5
Embodiment 2 is rich selenium commodity abalones in culturing the pond through 120 days breed result.
If the method according to embodiment 2 (group b-2) continued to culture 150 days to 180 days, detect the various features value of rich selenium commodity abalone respectively and see table 5.
The result shows; Along with the increase of culturing fate; Rich selenium commodity abalone various features value is also along with increase; Cultured 150 days and 180 days average shell length, average weight, SOD vigor, GPX vigor, abalone selenium content, survival rate and rate of body weight gain comparison increase respectively according to group to some extent, wherein selenium content increases the most obvious.
Table 5. different breeding fate is to the influence of rich selenium commodity abalone
Embodiment 6
Bright sea-tangle is broken into less than 0.5cm with the hock type pulverizer
2The sea-tangle sheet is referred to as the slurry sea-tangle.When sea-tangle is ground into slurry, to compare with the strip sea-tangle, specific area increases many, helps abalone contact feed, increases the chance of edible sea-tangle.Yet the slurry sea-tangle is also flowed out with the seawater of discharging easily, causes the loss of feed.Therefore with embodiment 6 and 7, prove respectively:
According to the method breeding selenium rich commodity abalone of embodiment 1, difference is, changes feed into slurry sea-tangle, result such as table 6 by the strip sea-tangle.
Table 6 is the result prove, the experimental group of amount of water 985mL (group a-5), and its rich selenium commodity characteristic value all is higher than other groups.Proof adds the disease resistance and increase output that nanometer selenium helps rich selenium commodity abalone.Compare with control group, survival rate improves 3.3%, and rate of body weight gain improves 2.0%; Selenium content than strip sea-tangle feed group has improved 0.086mg/kg, and survival rate improves 1.3%, and rate of body weight gain improves 1.9%.The characteristic value of the commodity abalone of slurry sea-tangle experimental group is all high than strip sea-tangle feed control group, explains that the slurry sea-tangle more helps abalone and takes in feed.
Table 6. amount of water is to 0.50mg/kg
The slurry sea-tangleCulture 120 days rich selenium commodity abalone influence
Embodiment 7
According to the method breeding selenium rich commodity abalone of embodiment 2, difference is, changes feed into slurry sea-tangle, result such as table 7 by the strip sea-tangle.
Table 7 is the result prove, the experimental group of amount of water 970mL (group b-5), and its rich selenium commodity characteristic value all very significantly is higher than other groups.Compare with control group, selenium content has improved 0.333mg/kg, and survival rate improves 4.2%, and rate of body weight gain improves 2.1%; Selenium content than strip sea-tangle feed group has improved 0.043mg/kg, and survival rate improves 1.5%, and rate of body weight gain improves 0.9%.The characteristic value of the commodity abalone of slurry sea-tangle experimental group is all high than strip sea-tangle feed control group, explains that the slurry sea-tangle more helps abalone and takes in feed.
Table 7. amount of water is to 1.00mg/kg
The slurry sea-tangleCulture 120 days rich selenium commodity abalone influence
Embodiment 8
According to the method breeding selenium rich commodity abalone of embodiment 3, difference is, changes feed into slurry sea-tangle, result such as table 8 by the strip sea-tangle.
Table 8 is the result prove, the experimental group of amount of water 955mL (group c-5), and some characteristic value of its rich selenium commodity abalone very significantly is higher than other groups.Compare with control group, selenium content has improved 0.438mg/kg, and survival rate improves 3.7%, and rate of body weight gain improves 2.0%; Though selenium content increases than embodiment 7 height, this is because the result that the nanometer selenium input amount increases.But survival rate and rate of body weight gain are all little than the amplitude that embodiment 7 improves, and explain that the nanometer selenium dosage begins to influence the growth of rich selenium commodity abalone.
Table 8. amount of water is to 1.50mg/kg
The slurry sea-tangleCulture 120 days rich selenium commodity abalone influence
Embodiment 9
According to the method breeding selenium rich commodity abalone of embodiment 4, difference is, changes feed into slurry sea-tangle, result such as table 9 by the strip sea-tangle.
Table 9 is the result prove, the experimental group of amount of water 940mL (group d-5), and some characteristic value of its rich selenium commodity abalone very significantly is higher than other groups.Compare with control group, especially the selenium content recruitment is high especially, is 0.606mg/kg, is 3.5 times of control group, and survival rate improves 3.0%, and rate of body weight gain improves 1.9%; Selenium content, survival rate and rate of body weight gain than strip sea-tangle feed group (group d-2) have improved 0.075mg/kg, 1.3% and 1.1% respectively; Increase 0.373mg/kg than the visible selenium content of embodiment 7 (group b-5); But survival rate reduces by 1.2%, and rate of body weight gain reduces by 0.2%.Originally calculated from the one-tenth of nanometer dosage, exceed 1 times more than embodiment, and the output of rich selenium commodity abalone was less than embodiment 7.
Table 9. amount of water is to 2.00mg/kg
The slurry sea-tangleCulture 120 days rich selenium commodity abalone influence
Embodiment 10
Embodiment 7 is rich selenium commodity abalones in culturing the pond through 120 days breed result.
If the method according to embodiment 7 (group b-5) continued to culture 150 days to 180 days, detect the various features value of rich selenium commodity abalone respectively and see table 10.
The result shows that along with the increase of culturing fate, rich selenium commodity abalone various features value is also along with increase, and amplification is higher than the effect of strip sea-tangle forage feed.Culture after 150 days and 180 days, its average shell length, average weight, SOD vigor, GPX vigor, abalone selenium content, survival rate and rate of body weight gain increase respectively than control group, strip sea-tangle feed breed group to some extent.Therefore can be according to the abalone market price and demand, account cost and benefit, the fate that decision is cultured.
Table 10. different breeding fate is to the influence of rich selenium commodity abalone
In sum:
(1) uses strip or the slurry sea-tangle of nanometer selenium dosage as 0.50-2.00mg/kg; The rich selenium commodity abalone selenium content of being raised is 0.205-0.706mg/kg and 0.282-0.781mg/kg respectively; Belong to rich selenium abalone; And meet the limit the quantity of requirement of sanitary standard (GB13105-91) Se content≤1.00mg/kg of selenium in the state food, the commodity abalone of safe selenium-complement is provided for people.
(2) SOD vigor, GPX vigor constantly increase in its body of breeding selenium rich commodity abalone process, explain that the anti-oxidant immunity of self improves, and survival rate rises.The rich selenium commodity abalone that rich selenium strip sea-tangle is raised; Its survival rate rises to 91.3% from 85.0%; The rich selenium commodity abalone that rich selenium slurry sea-tangle is raised, its survival rate rise to 92.7% from 88.5%, and rate of body weight gain rises to 19.6% and 21.5% respectively; Be 1.3 and 1.4 times of control group, its economic benefit is appreciable.
(3) instance cited according to the embodiment of the invention; Those skilled in the art can add nanometer selenium 1.00-1.50mg/kg, after drying through combining prior art or common practise in the bait of sea cucumber, fish, shrimp etc.; By different rate of charges; Feed sea cucumber, fish, shrimp etc., promptly get the rich selenium sea cucumber of selenium content 0.50-1.00mg/kg, rich selenium fish and rich selenium shrimp, or the like.Owing to improved anti-oxidant immunity, reduce the incidence of disease, so can reduce or the use amount of added with antibiotic medicine not, also improve 5-15% output simultaneously, in concrete production application, its economic benefit increases significantly.
Claims (9)
1. the Herba Zosterae marinae rich in selenium feed of a breeding selenium rich abalone; It is characterized in that, prepare through following steps: to particle diameter is that 100～140nm, concentration are in the red nano selenium solution of 0.50mg/mL, adds the water of 7～100 times of volumes; Make the nanometer selenium dilution; Again in nanometer selenium: bright sea-tangle is the ratio of 0.50～2.00mg: 1kg, whole dilutions evenly is sprayed on the bright sea-tangle of strip or pulp-like, processes after sea-tangle is dried.
2. Herba Zosterae marinae rich in selenium feed according to claim 1 is characterized in that, the nanometer selenium dilution is 1: 16～67 to make by the volume ratio of red nano selenium solution and water.
3. Herba Zosterae marinae rich in selenium feed according to claim 2 is characterized in that, the nanometer selenium dilution is 1: 30～35 to make by the volume ratio of red nano selenium solution and water.
4. Herba Zosterae marinae rich in selenium feed according to claim 3 is characterized in that, the nanometer selenium dilution is to make at 30: 970 by the volume ratio of red nano selenium solution and water.
5. according to each described Herba Zosterae marinae rich in selenium feed in the claim 1,2,3 or 4, it is characterized in that in nanometer selenium: bright sea-tangle is that the ratio of 0.80～1.20mg: 1kg is sprayed onto the nanometer selenium dilution on the bright sea-tangle.
6. Herba Zosterae marinae rich in selenium feed according to claim 5 is characterized in that, in nanometer selenium: bright sea-tangle is that the ratio of 1.00mg: 1kg is sprayed onto the nanometer selenium dilution on the bright sea-tangle.
7. the method for a breeding selenium rich abalone is characterized in that, utilizes the described Herba Zosterae marinae rich in selenium feed of claim 1 feeding sea-ear.
8. the method for breeding selenium rich abalone according to claim 7 is characterized in that, concrete method for breeding comprises:
1. get concentration 0.50mg/mL particle diameter 100～140nm red nano selenium solution 30.0mL, add water 970mL dilution evenly after, all evenly be sprayed at the bright sea-tangle surface of 15kg strip or slurry, after sea-tangle is dried, obtain Herba Zosterae marinae rich in selenium;
2. utilize the Herba Zosterae marinae rich in selenium feeding sea-ear 120～180 days that 1. step obtain, the raising condition is:
In the indoor culture cement pit, Chi Dipu goes into to culture the Bao lamina affixad, adds 3000/15m of abalone
2, piling the clean sea water after deposition, filtration treatment in the pond, seawater salt content 30 ‰, pH are 7.8～8.2; Dissolved oxygen 6～8mg/L; Water temperature remains on 10-24 ℃ in breeding process, the feed of throwing something and feeding during 16-17 every day; Inventory is 10% of an abalone TBW, and change breeding seawater next day during 9-11, washes away pool wall.
9. the application of method as claimed in claim 7 aspect production rich selenium sea cucumber, rich selenium fish or rich selenium shrimp.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210078956.4A CN102599374B (en) | 2012-03-23 | 2012-03-23 | Selenium-enriched kelp feed and application thereof to culture of selenium-enriched abalone |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210078956.4A CN102599374B (en) | 2012-03-23 | 2012-03-23 | Selenium-enriched kelp feed and application thereof to culture of selenium-enriched abalone |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102599374A true CN102599374A (en) | 2012-07-25 |
| CN102599374B CN102599374B (en) | 2014-11-26 |
Family
ID=46517450
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201210078956.4A Active CN102599374B (en) | 2012-03-23 | 2012-03-23 | Selenium-enriched kelp feed and application thereof to culture of selenium-enriched abalone |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102599374B (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102805047A (en) * | 2012-08-15 | 2012-12-05 | 苏州硒谷科技有限公司 | Cultivating method for selenium-rich feed and selenium-rich abalone |
| CN108739562A (en) * | 2018-05-25 | 2018-11-06 | 佛山推启农业研究院（普通合伙） | A kind of abalone breeding method |
| CN110301378A (en) * | 2019-07-29 | 2019-10-08 | 广西壮族自治区水产科学研究院 | A kind of selenium-rich Tilapia mossambica production cultural method |
| CN114365711A (en) * | 2022-01-24 | 2022-04-19 | 威海金牌生物科技有限公司 | Selenium-rich oyster cultivation method |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1126552A (en) * | 1995-01-09 | 1996-07-17 | 淄博市营养保健食品研究所 | Disease-preventing mixed bait for abalone and its prepn |
| CN101313729A (en) * | 2008-06-20 | 2008-12-03 | 田福平 | Organic biological feedstuff additive containing nano-selenium, zinc oxide, preparation and use method thereof |
| CN101341939A (en) * | 2008-09-10 | 2009-01-14 | 大连太平洋海珍品有限公司 | Abalone compound feed |
| CN101411390A (en) * | 2008-11-14 | 2009-04-22 | 兰传强 | Method for storing abalone bait |
| CN101642194A (en) * | 2009-09-03 | 2010-02-10 | 吴敦虎 | Nutrient solution for breeding selenium rich snail, breeding method and selenium rich snail product |
-
2012
- 2012-03-23 CN CN201210078956.4A patent/CN102599374B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1126552A (en) * | 1995-01-09 | 1996-07-17 | 淄博市营养保健食品研究所 | Disease-preventing mixed bait for abalone and its prepn |
| CN101313729A (en) * | 2008-06-20 | 2008-12-03 | 田福平 | Organic biological feedstuff additive containing nano-selenium, zinc oxide, preparation and use method thereof |
| CN101341939A (en) * | 2008-09-10 | 2009-01-14 | 大连太平洋海珍品有限公司 | Abalone compound feed |
| CN101411390A (en) * | 2008-11-14 | 2009-04-22 | 兰传强 | Method for storing abalone bait |
| CN101642194A (en) * | 2009-09-03 | 2010-02-10 | 吴敦虎 | Nutrient solution for breeding selenium rich snail, breeding method and selenium rich snail product |
Non-Patent Citations (2)
| Title |
|---|
| 《微量元素与健康研究》 20090930 田福平等 "生物纳米硒饲料添加剂对淡水养殖鱼类影响的研究" 第26卷, 第5期 * |
| 田福平等: ""生物纳米硒饲料添加剂对淡水养殖鱼类影响的研究"", 《微量元素与健康研究》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102805047A (en) * | 2012-08-15 | 2012-12-05 | 苏州硒谷科技有限公司 | Cultivating method for selenium-rich feed and selenium-rich abalone |
| CN108739562A (en) * | 2018-05-25 | 2018-11-06 | 佛山推启农业研究院（普通合伙） | A kind of abalone breeding method |
| CN110301378A (en) * | 2019-07-29 | 2019-10-08 | 广西壮族自治区水产科学研究院 | A kind of selenium-rich Tilapia mossambica production cultural method |
| CN114365711A (en) * | 2022-01-24 | 2022-04-19 | 威海金牌生物科技有限公司 | Selenium-rich oyster cultivation method |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102599374B (en) | 2014-11-26 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Venkataramiah et al. | Effect of protein level and vegetable matter on growth and food conversion efficiency of brown shrimp | |
| CN103380872B (en) | Artificial pellet feed for adult turbot | |
| CN104026413B (en) | The special slow heavy property feed of a kind of bighead and preparation technology thereof | |
| CN107927458B (en) | Immunity-enhanced penaeus vannamei feed and preparation method thereof | |
| CN102972660A (en) | Chinese herbal medicine compound bait for improving growing power, immunity and disease resistance of stichopus japonicus | |
| CN103621448A (en) | Pond poly-culture method for tilapia mossambica and litopenaeus vannamei | |
| CN104664164A (en) | Feed additive capable of reducing white spot syndrome virus disease of penaeus vannamei boone | |
| CN107494340A (en) | A kind of ecological fish culture method | |
| CN102599374B (en) | Selenium-enriched kelp feed and application thereof to culture of selenium-enriched abalone | |
| CN101591040A (en) | Method for preventing and controlling water bloom pollution organisms in eutrophicated water body | |
| CN102132776B (en) | All-natural feed additive for crustacean and preparation method thereof | |
| CN102106863B (en) | Water sprinkling anti-stress agent for aquaculture | |
| CN106212392A (en) | A kind of improve wheel animalcule growth and breeding ability and strengthening its as the method for food nutrition | |
| CN101273760B (en) | Black rockfish phagostimulant preparation method and usage method | |
| CN108782367A (en) | The method and feed and preparation method and application of raising pseudosciaena crocea larvae survival rate | |
| CN102657114A (en) | Mixed culture method for hippocampi and sea cucumbers | |
| CN105533167A (en) | Alga oligosaccharide fish feed additive | |
| CN111248117A (en) | Breeding method of acrossocheilus fasciatus | |
| CN103734544B (en) | A kind of Chinese herbal medicine food attractant for Liza haematocheila | |
| Xie et al. | Aerated sea mud is beneficial for post-nursery culture of early juvenile sea cucumber Apostichopus japonicus (Selenka) | |
| CN101480232A (en) | Feed addictive for promoting upgrowth of Litopenaeus vannamei under low-salt environment | |
| CN101455281A (en) | Preparation method of green algae enteromorpha as turbot attractant | |
| CN113854441A (en) | Special functional compound feed for siniperca chuatsi and application of special functional compound feed | |
| LU502707B1 (en) | Sustained-release pellet feed for southward transplanted apostichopus japonicus in overwintering period and its preparation method | |
| Pratiwy et al. | The effect of enzymatic Sargassum extract on feed consumption, feed utlitization efficiency, and growth rate of Nile tilapia (Oreochromis niloticus) |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |