CN106212392A - A kind of improve wheel animalcule growth and breeding ability and strengthening its as the method for food nutrition - Google Patents

A kind of improve wheel animalcule growth and breeding ability and strengthening its as the method for food nutrition Download PDF

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Publication number
CN106212392A
CN106212392A CN201610628037.8A CN201610628037A CN106212392A CN 106212392 A CN106212392 A CN 106212392A CN 201610628037 A CN201610628037 A CN 201610628037A CN 106212392 A CN106212392 A CN 106212392A
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wheel animalcule
rich
growth
biological product
aquatic
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刘建国
李虎
董乃畅
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Institute of Oceanology of CAS
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Institute of Oceanology of CAS
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/033Rearing or breeding invertebrates; New breeds of invertebrates

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Zoology (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Fodder In General (AREA)

Abstract

The present invention is subordinate to Aquatic product field, relates to a kind of method improving wheel animalcule growth and breeding power and strengthening food nutrition.In the incubation of aquatic feed wheel animalcule, by adding the microalgae biological product rich in natural bioactivity substance, and then improve the growth and breeding ability of wheel animalcule, strengthen its nutrition as bait.The present invention, with the microalgae rich in bioactive substance as pure natural biological product, has been ensured the natural sex of aquatic products, has used safety, have no side effect by food chain transmission;Microalgae biological product do not contain only various bioactivators, also rich in other nutrition and energy matters such as certain polysaccharide and protein, can comprehensively improve production effect.The present invention is by antioxygenic property, radioprotective power and the nutritive effect of natural active matter, can improve and feed aquatic animal Fish, shrimps, Eriocheir sinensis class and the survival rate of other aquatic product animal raise seedling, growth rate and resistance against diseases, comprehensive raising aquaculture efficiency.

Description

A kind of improve wheel animalcule growth and breeding ability and strengthening its as the method for food nutrition
Technical field
The present invention is subordinate to Aquatic product field, relates to a kind of method improving wheel animalcule growth and breeding power and strengthening food nutrition.
Background technology
Along with society's development and growth in the living standard, the demand of the aquatic products such as fish shrimp crab is constantly increased by people Adding, the catch of the most above-mentioned aquatic products reduces year by year, and Efforts To Develop artificial cultivation provides more aquatic products, satisfies social needs It it is the major trend of current and following aquatic products industry development.
But, in current Fish, shrimps, Eriocheir sinensis class and other aquaculture of aquatic animal, particularly at nursery stage above-mentioned water Produce the germling of animal generally there is hatching life rate be relatively low, poor growth, hypoimmunity or disease occur even dead etc. be Row problem.One of major reason is that the bait that artificial cultivation is provided is the most single, abundant in malnutrition, with natural environment Various natural bait has the biggest difference.How by simulation nature food chain (net), (particularly opening is fresh and alive to improve bait Bait) quality be aquaculture new proposition and need break through key technical problem.
Wheel animalcule (Rotifer) is the open-mouthed bait of most of aquatic animal germling, for trochelminth Rotatoria (Rotifera Or Rotaria) the general name of a small invertebrates of class.Wheel animalcule body is small, is about 0.04-2 millimeter, is usually no more than 0.5 millimeter.Rotifer distribution is wide, most free livings, also has parasitism, i.e. has individual Ye You colony.Wheel animalcule health is elongated, is divided into Head, trunk and afterbody.Head is made up of 1-2 circle cilium by one, the wheel disc that can rotate, shape such as wheel thus cry wheel animalcule.Many The same direction of cilium hoop on number wheel animalcule roller dish rotates, and makes water form vortex and absorb antibacterial, unicellular microalgae and chip Granule.Wheel animalcule bisexualism, oviparity, it mostly is parthenogenesis, it is possible to syngenesis occurs.The proliferative speed that wheel animalcule is exceedingly fast because of it, Volume of production is the highest.At nature, wheel animalcule is distributed widely in all kinds of light, the salt water water body such as lake, pond, rivers, coastal waters, can seek Swimming or facultative life of swimming, dwells, adheres to or set life in the most separately battalion end having.
At nature aquatic ecosystem, wheel animalcule is the open-mouthed bait of most of aquatic animal germling, is maintaining Aquatic Ecosystem 2 Structure function is played an important role, to ensureing that fish production also has the irreplaceable important function of performance.At present manually In aquaculture, no matter from the wheel animalcule of capture naturally, or the wheel animalcule of artificial cultivation all can not meet the great demand amount of Aquatic product, Meanwhile, in artificial cultivation wheel animalcule, unicellular microalgae supply is restricted, and bait of throwing something and feeding depends on yeast, bean milk, Testa Tritici Deng.But above-mentioned substance only meets the energy requirement of wheel animalcule, and cannot enough offer astaxanthins, carotenoid, EPA and DHA The natural active matters such as polyunsaturated fatty acid, the wheel animalcule bait produced be difficult to meet aquatic animal Fish, shrimps, Eriocheir sinensis class with And the demand that other aquatic animal is to above-mentioned active substance, cause aquatic animal survival rate, growth rate and resistance against diseases to be subject to Have a strong impact on, thus govern the further development of culture fishery
Summary of the invention
For aquaculture and the Present nutrition status of bait wheel animalcule thereof, the present invention proposes a kind of raising wheel animalcule growth and breeding ability Method with strengthening food nutrition.
For achieving the above object, the used technical scheme of the present invention is:
A kind of method improving wheel animalcule growth and breeding ability and strengthening food nutrition, in the incubation of aquatic feed wheel animalcule In, by adding the microalgae biological product rich in natural bioactivity substance, and then improve wheel animalcule growth rate and fertility, And strengthen antioxygenic property and the nutritive effect of wheel animalcule bait.
The microalgae goods rich in bioactive substance added, there is certain domain of walker in its using dosage, this model Enclose and be based primarily upon its bioactive substance content difference and cause there is certain difference.Generally bioactive substance content is high, Then addition is few;Otherwise, the most.It is said that in general, the microalgae biological product consumption rich in bioactive substance added accounts for and raises Feed the 1.0%-30.0% of the wheel animalcule abundance of food, optimize dosage 2.0%-20.0%, optimal dose 5.0%-10.0%.
The described microalgae biological product rich in natural bioactivity substance are that one or more are rich in natural bioactive thing The algae powder of matter, fresh algae mud or the biological product of algae-residue;Wherein, biological product be cell wall breaking or without cell wall breaking.
Described natural bioactivity substance is rich in a kind or several natural bioactivity substance;Natural bioactive thing Matter is one or more unsaturated fatty acids in astaxanthin, carotenoid and AA, EPA, DHA.
The described microalgae biological product rich in natural bioactivity substance are the Haematococcus Pluvialis rich in astaxanthin, rich in Radix Dauci Sativae The salt alga of element, rich in one or more biological product split in kettle algae of DHA unsaturated fatty acid, wherein, biological product are thin Born of the same parents' breaking cellular wall or without cell wall breaking.
A kind of application utilizing the method improving wheel animalcule growth and breeding power and strengthening food nutrition, utilizes described method to raise Wheel animalcule obtains the wheel animalcule that growth rate, fertility, antioxygenic property and nutritive effect are strong, using gained wheel animalcule as bait, makees The application of aquatic biological feed is fed for preparation.
Specifically: first with microalgae biological product fortification wheel animalcule bait, reapply above-mentioned fortification wheel animalcule bait Feed Fish, shrimps, Eriocheir sinensis class and other aquatic animal, increase Fish, shrimps, Eriocheir sinensis class and the one-tenth of other aquatic product animal raise seedling Motility rate, growth rate and resistance against diseases, strengthen radioprotective power and the Strong oxdiative performance of aquatic animal, increase comprehensive production efficiency.
Characteristic and the advantage of the present invention are:
The present invention, in the incubation of aquatic feed wheel animalcule, first passes through interpolation appropriate rich in natural bioactivity substance Microalgae biological product, improve wheel animalcule growth and breeding ability, strengthen its food nutrition;Then, then by this fortification wheel animalcule Bait is applied in aquaculture, and purpose meets aquatic animal Fish, shrimps, Eriocheir sinensis class and other aquatic animal to above-mentioned activity The primary demand of material, thus realize increasing the survival rate of aquatic product animal raise seedling, growth rate and resistance against diseases, increase comprehensive raw Produce the purpose of efficiency;Particularly as follows:
1., with the microalgae rich in bioactive substance as pure natural biological product, aquatic products have been ensured by food chain transmission Natural sex, use safety, have no side effect;Microalgae biological product do not contain only various bioactivators, also rich in necessarily Polysaccharide and other nutrition and the energy matter such as protein, can comprehensively improve production effect.
2. microalgae natural active matter such as natural astaxanthin, carotenoid, EPA and DHA polyunsaturated fatty acid etc., many Existing in ester matter mode, its stability is strong, therefore it is high to feed wheel animalcule absorption efficiency;
3. microalgae biological product are with algae mud as a raw material, can reduce dry link and energy resource consumption, reduce production cost;With algae Slag is biological product, not only can realize equivalence effect of the present invention, and the environment that it also avoid waste sludge discharge and be likely to result in is dirty Dye pressure.
4. the present invention is by antioxygenic property, radioprotective power and the nutritive effect of natural active matter, can improve and feed Aquatic animal Fish, shrimps, Eriocheir sinensis class and the survival rate of other aquatic product animal raise seedling, growth rate and resistance against diseases, comprehensively carry High aquaculture efficiency.
Accompanying drawing description
The different bait that utilizes that Fig. 1 provides for the embodiment of the present invention feeds the culture effect figure of wheel animalcule;Wherein, A, micro-plan ball Algae feeds group;B, yeast feeds group;C, micro-plan ball algae+feed group rich in the Haematococcus Pluvialis of astaxanthin.
Specific embodiment
Further illustrate the invention of the application below in conjunction with embodiment, but embodiment is not construed as the limit to right of the present invention Fixed, the usual variations and alternatives etc. that the person skilled of this area is carried out, also belong to the scope that the invention is protected.
Embodiment 1
Take the cultivation water tank of 3 10 liters, be added to the sea water of 8 liter 25 ‰, and inoculate isopycnic wheel animalcule (16/ Milliliter), abundant aerobic culture 8 days.Wherein in an incubator, every day adds the fresh micro-plan ball algae mud of 3.0g;And train to another Support micro-plan ball algae fresh algae mud and 1.5% Haematococcus Pluvialis fresh algae mud mixed product, wherein chlorella and the Haematococcus Pluvialis of weight such as adding in case Quality ratio be 4:1 (i.e. Haematococcus Pluvialis accounts for the 20% of total bait);It addition, in the 3rd incubator every day add the fresh of 2.0g Yeast, as comparison (seeing Fig. 1).
Result shows: the wheel animalcule growth and breeding feeding microalgae two groups is better than feeding yeast group, wherein feed micro-plan ball algae and The wheel animalcule growth of Haematococcus Pluvialis algae mud group is best, and proliferative speed is the fastest, and wheel animalcule density is the highest, and every milliliter contains 160 wheel animalcules, than list Feed micro-plan ball algae group (100/milliliter) the highest by 60%;And individually one group of wheel animalcule growth and breeding speed of feeding yeast is the slowest, wheel Worm yield is minimum, and its wheel animalcule density only has 50/milliliter, feeds about the 50% of micro-plan ball algae group wheel animalcule Density Density for single.
Equally, no matter cherishing ovum wheel borer population, wherein bosom ovum sum, feeds the wheel animalcule group of microalgae apparently higher than feeding yeast Group.Carrying a lot of ovum the wheel animalcule feeding microalgae group, some carries 2 even more ovum (ginseng Figure 1A), and at feeding yeast The wheel animalcule of group does not the most cherish ovum (ginseng Figure 1B).
It addition, the wheel animalcule bosom ovum rate feeding micro-plan ball algae and Haematococcus Pluvialis algae mud group is the highest, the individuality that some individuality is less Also nourishing 1-2 ovum (ginseng Fig. 1 C), the most no matter cherish ovum wheel borer population, wherein bosom ovum sum binomial index, feeds at three In group the highest.But, just bosom ovum wheel animalcule is at the ratio of total group's number, feeds micro-plan ball algae group basic with Haematococcus Pluvialis group with micro-plan ball algae Unanimously, about 20% is accounted for.
Embodiment 2
Separately take the cultivation water tank of 3 10 liters, be added to the sea water of 8 liter 25 ‰, and inoculate isopycnic wheel animalcule (15 Individual/milliliter), abundant aerobic culture 6 days.Wherein in an incubator, every day adds the fresh micro-plan ball algae algae mud of 3.0g and (is roughly equal to 0.86g dry weight);And in another incubator, micro-plan ball algae fresh algae mud and the 0.5% Haematococcus Pluvialis algae-residue mixing system of weight such as add Product, wherein, chlorella addition accounts for the 70% of total interpolation quality, and Haematococcus Pluvialis addition accounts for the 30% of total interpolation quality;It addition, to 3rd incubator adds the fresh yeast of 3.0g, as comparison every day.
Experimental result shows the similar effect with embodiment 1: the wheel animalcule growth and breeding effect feeding microalgae two groups is the best In independent feeding yeast group, the wheel animalcule growth wherein feeding micro-plan ball algae and Haematococcus Pluvialis algae-residue group is best, and proliferative speed is the fastest, wheel Worm density is the highest, and every milliliter contains 100 wheel animalcules, higher by about 60% than single hello micro-plan ball algae group (58/milliliter) is wanted equally;And Individually one group of wheel animalcule growth and breeding speed of feeding yeast is the slowest, and wheel animalcule yield is minimum, and its wheel animalcule density feeds micro-plan ball than single Algae group is low by about 50%.
Equally, no matter cherish ovum wheel borer population, or wheel animalcule colony bosom ovum sum, and bosom ovum wheel animalcule account for ratio in colony with The result that embodiment 1 is similar.I.e. feed the wheel animalcule group of microalgae apparently higher than feeding yeast group, and feed micro-plan ball algae and red ball Algae algae-residue group bosom ovum wheel borer population, bosom ovum sum in three experimental grouies the highest, but its bosom ovum wheel animalcule is at the ratio of total group's number, with Individually feed micro-plan ball algae group substantially uniform.
Embodiment 3
Further with Haematococcus Pluvialis breaking cellular wall algae powder and the Haematococcus Pluvialis breaking cellular wall fresh algae mud of 2.0% of astaxanthin-containing 4.0%, respectively Mix with micro-plan ball algae, again carry out above-mentioned wheel animalcule administering transgenic.Also the similar similar result of case study on implementation 1 and 2 is obtained.Its In, after Haematococcus Pluvialis algae powder, algae mud breaking cellular wall, its granule is less, and more conducively wheel animalcule ingests and absorbs, growth and breeding and the bosom to wheel animalcule Ovum increased, and increased the growth and breeding of wheel animalcule and bosom ovum although adding two kinds of breaking cellular wall compositions, but between the two at system Meter not yet observes significant difference on learning.
Embodiment 4
Further with said method, amplification culture wheel animalcule (brachionus plicatilis) scale, after results, it is used for shrimp seed rearing, Result shows thrown something and fed prawn Seedling, and the prawn shrimp Seedling rate of development that relatively conventional art is cultivated is fast, growing-seedling period shortens, and shrimp Seedling is more Homogeneous and have more vigor.
Above-described embodiment shows: in the incubation of aquatic feed wheel animalcule, by adding rich in natural bioactive thing The microalgae biological product of matter, can increase growth and the fertility of wheel animalcule, the trophic function of strengthening wheel animalcule bait, and then by this its It is applied in aquaculture, increases aquatic product animal raise seedling effect.

Claims (7)

1. the method improving wheel animalcule growth and breeding ability and strengthening food nutrition, it is characterised in that: aquatic feed wheel animalcule Incubation in, by adding rich in the microalgae biological product of natural bioactivity substance, and then the growth improving wheel animalcule is numerous Grow ability, strengthen its nutrition as bait.
2. the method improving wheel animalcule growth and breeding power and strengthening food nutrition as described in claim 1, it is characterised in that: described The 1.0%-30.0% feeding wheel animalcule bait gross mass is accounted for rich in the interpolation of the microalgae biological product of natural bioactivity substance.
3. the method improving wheel animalcule growth and breeding power and strengthening food nutrition as described in claim 2, it is characterised in that: described The 2.0%-20.0% feeding wheel animalcule bait gross mass is accounted for rich in the addition of the microalgae biological product of natural bioactivity substance.
4. the method improving wheel animalcule growth and breeding power and strengthening food nutrition as described in claim 1-3 any one, it is special Levy and be: the described microalgae biological product rich in natural bioactivity substance are that one or more are rich in natural bioactivity substance Algae powder, fresh algae mud or the biological product of algae-residue;Wherein, biological product be cell wall breaking or without cell wall breaking.
5. the method improving wheel animalcule growth and breeding power and strengthening food nutrition as described in claim 4, it is characterised in that: described Natural bioactivity substance is rich in a kind or several natural bioactivity substance;Natural bioactivity substance be astaxanthin, One or more unsaturated fatty acids in carotenoid, AA, EPA, DHA.
6. the method improving wheel animalcule growth and breeding power and strengthening food nutrition as described in claim 4, it is characterised in that: described Rich in the microalgae biological product of natural bioactivity substance be the Haematococcus Pluvialis rich in astaxanthin, rich in carotene salt alga, rich in One or more biological product split in kettle algae of DHA unsaturated fatty acid, wherein, biological product be cell wall breaking or without Cell wall breaking.
7. utilize the application improving wheel animalcule growth and breeding power and the method for strengthening food nutrition described in claim 1, its It is characterised by: utilize described method to raise wheel animalcule and obtain the wheel that growth rate, fertility, antioxygenic property and nutritive effect are strong Worm, using gained wheel animalcule as bait, feeds the application of aquatic biological feed as preparation.
CN201610628037.8A 2016-08-01 2016-08-01 A kind of improve wheel animalcule growth and breeding ability and strengthening its as the method for food nutrition Pending CN106212392A (en)

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107794115A (en) * 2017-12-06 2018-03-13 哈尔滨工业大学深圳研究生院 A kind of method that broken wall assisted extraction microalgae grease is digested using protozoal predation
CN110999836A (en) * 2019-12-31 2020-04-14 海南晨海水产有限公司 Nutrition strengthening method for promoting growth of penaeus orientalis larvae
CN113396843A (en) * 2021-07-12 2021-09-17 海南绿藻世界生物科技有限公司 Culture medium rich in microalgae and method for culturing L-type rotifers
CN116076405A (en) * 2022-12-30 2023-05-09 中国科学院青岛生物能源与过程研究所 Method for cultivating fish, shrimp and shellfish and artemia by utilizing nervonic acid microalgae

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CN101653106A (en) * 2009-09-22 2010-02-24 中国水产科学研究院渔业机械仪器研究所 Closed circulating rotifer continuous culturing system
CN101810246A (en) * 2009-11-13 2010-08-25 厦门汇盛生物有限公司 Composite nutrition enhancer and preparation method thereof
CN105613433A (en) * 2016-03-03 2016-06-01 武汉康立斯科技发展有限公司 Method and system for culturing rotifer in freshwater

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1653938A (en) * 2005-03-09 2005-08-17 青岛森淼实业有限公司 Mirco algal food nutrient intensifying agent prescription and its preparing process
CN101653106A (en) * 2009-09-22 2010-02-24 中国水产科学研究院渔业机械仪器研究所 Closed circulating rotifer continuous culturing system
CN101810246A (en) * 2009-11-13 2010-08-25 厦门汇盛生物有限公司 Composite nutrition enhancer and preparation method thereof
CN105613433A (en) * 2016-03-03 2016-06-01 武汉康立斯科技发展有限公司 Method and system for culturing rotifer in freshwater

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107794115A (en) * 2017-12-06 2018-03-13 哈尔滨工业大学深圳研究生院 A kind of method that broken wall assisted extraction microalgae grease is digested using protozoal predation
CN110999836A (en) * 2019-12-31 2020-04-14 海南晨海水产有限公司 Nutrition strengthening method for promoting growth of penaeus orientalis larvae
CN113396843A (en) * 2021-07-12 2021-09-17 海南绿藻世界生物科技有限公司 Culture medium rich in microalgae and method for culturing L-type rotifers
CN116076405A (en) * 2022-12-30 2023-05-09 中国科学院青岛生物能源与过程研究所 Method for cultivating fish, shrimp and shellfish and artemia by utilizing nervonic acid microalgae

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