CN111248117A - Breeding method of acrossocheilus fasciatus - Google Patents
Breeding method of acrossocheilus fasciatus Download PDFInfo
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- CN111248117A CN111248117A CN202010045170.7A CN202010045170A CN111248117A CN 111248117 A CN111248117 A CN 111248117A CN 202010045170 A CN202010045170 A CN 202010045170A CN 111248117 A CN111248117 A CN 111248117A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/33—Heterocyclic compounds
- A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
- A61K31/435—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with one nitrogen as the only ring hetero atom
- A61K31/44—Non condensed pyridines; Hydrogenated derivatives thereof
- A61K31/445—Non condensed piperidines, e.g. piperocaine
- A61K31/4523—Non condensed piperidines, e.g. piperocaine containing further heterocyclic ring systems
- A61K31/454—Non condensed piperidines, e.g. piperocaine containing further heterocyclic ring systems containing a five-membered ring with nitrogen as a ring hetero atom, e.g. pimozide, domperidone
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/04—Peptides having up to 20 amino acids in a fully defined sequence; Derivatives thereof
- A61K38/08—Peptides having 5 to 11 amino acids
- A61K38/09—Luteinising hormone-releasing hormone [LHRH], i.e. Gonadotropin-releasing hormone [GnRH]; Related peptides
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- A61K38/22—Hormones
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- A61K38/22—Hormones
- A61K38/24—Follicle-stimulating hormone [FSH]; Chorionic gonadotropins, e.g. HCG; Luteinising hormone [LH]; Thyroid-stimulating hormone [TSH]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/0012—Galenical forms characterised by the site of application
- A61K9/0019—Injectable compositions; Intramuscular, intravenous, arterial, subcutaneous administration; Compositions to be administered through the skin in an invasive manner
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P15/00—Drugs for genital or sexual disorders; Contraceptives
- A61P15/08—Drugs for genital or sexual disorders; Contraceptives for gonadal disorders or for enhancing fertility, e.g. inducers of ovulation or of spermatogenesis
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Abstract
The invention belongs to the technical field of fishery breeding, and particularly relates to a method for breeding acrossocheilus fasciatus, which comprises the following specific steps: s1, selecting and strengthening parent fishes; s2, artificial induced spawning, injecting induced spawning drugs into parent fish for induced spawning, S3, artificial insemination, and fully mixing fish eggs and male fish semen according to the male-female ratio of 1:1-2 to form fertilized eggs; s4, artificial incubation, namely incubation in still water, intervening incubation and shelling periods of fertilized eggs by adjusting the temperature of culture water, and simultaneously adding a water quality modifier to improve the water quality; s5, raising seedlings, namely breeding the hatched fries in pond water to culture fries of lipped fries; the method has the advantages that the hatchability of fertilized eggs of the acrossocheilus fasciatus bred by the method is higher, the incidence rate of nutritional diseases of fry is reduced in the breeding process, and the high yield of the acrossocheilus fasciatus is ensured.
Description
Technical Field
The invention belongs to the technical field of fishery breeding, and particularly relates to a method for breeding acrossocheilus fasciatus.
Background
The liptophus belongs to the order of carps, carpidae, is usually distributed in mountainous areas in the south of China, lives in high turbulence environments such as mountain streams with clear water quality and middle and upper reaches of rivers, is fond of inhabiting in sandy bottom or rivers with high water flow turbulence, spawns at the water temperature of 18-27 ℃, the spawning season is generally 5-8 months, the breeding period is generally 6-7 months, and has development and utilization values.
Patent document No. CN106577393A discloses an artificial propagation method for culturing lipped fries, which is based on the on-site collection of juvenile lipped fries of wild lipped fries for training and cultivation until sexual maturity, uses exogenous oxytocic drugs of chorionic gonadotropin (HCG), luteinizing hormone releasing hormone analogue (LHRH-A3) and Diospyrone (DOM), promotes the gonads of the naturally mature lipped fries to further develop by one-shot injection, obtains fertilized eggs by dry fertilization, and incubates the fertilized eggs to come out of membranes by special incubation equipment in running water, thus solving the purpose of large-scale production. However, the method has low hatchability, cannot reduce the incidence rate of fry nutritional diseases in the breeding process, and has low survival rate and yield of the lipsticks.
Disclosure of Invention
The invention aims to solve the problems that the hatchability and the yield of the liptophagus arguta are low and the incidence of nutritional diseases of fry is high at present, and provides a breeding method of the liptophagus arguta.
In order to achieve the purpose, the invention adopts the following technical scheme:
a method for breeding liptophus fasciatus comprises the following specific steps:
s1, selecting and strengthening parent fishes, namely, putting the liptophus fasciatus with the weight of 50-200g into a net cage for culture, putting the male and female parent fishes into a spawning pond according to the quantity ratio of 1:1.2, and putting 4-5 tails of the female and male parent fishes in each cubic meter of pond water;
s2, artificially inducing spawning, injecting an oxytocic into the parent fish for inducing spawning, wherein the injection dosage of the male fish is 1/3-1/2 of that of the female fish, and placing the parent fish in a temporary rearing pond for shading and rearing after injecting the oxytocic;
s3, artificial insemination, namely, fully mixing the fish eggs and the male fish semen according to the male-female ratio of 1:1-2 to form fertilized eggs;
s4, artificial incubation, namely incubation in still water, putting fertilized eggs into incubators filled with culture water, putting 3 with the density of 3-6 granules/cm into each incubator for incubation, shading and standing for incubation for 160h by 150-fold, intervening the incubation and shelling periods of the fertilized eggs by adjusting the temperature of the culture water, changing water 1 time in the morning and evening each day, changing water 1/2 each time, and simultaneously adding a water quality modifier to improve the water quality;
s5, breeding seedlings, namely breeding the hatched fries in pond water, breeding the fries of the lipped fries, breeding 5000-6000 fries of the fries in pond water per cubic meter, wherein the dissolved oxygen is not less than 8mg/l, the pH value is 6.8-7.2, and adding a water quality modifier.
As a preferred scheme, the water quality modifier comprises, by mass, 10% -12% of liquorice, 15% of photosynthetic bacteria culture solution, 5% of molasses alcohol waste liquid, 3% of fulvic acid, 2% of EM (effective microorganisms), 2% of potassium trichloride, 20% -30% of folium isatidis and the balance of water.
Preferably, the temperature of the culture water in the step S4 is controlled to be 18-24 ℃.
Preferably, the dead eggs and unfertilized eggs are timely discharged in step S3, and the water is replaced every 3-5 hours after the dead eggs and unfertilized eggs are discharged, so as to keep the incubation water quality clean.
Preferably, in step S3, the fish eggs and the sperms of the male fish are squeezed into a wiped white porcelain basin and stirred, the well stirred fertilized eggs are placed into a plastic big basin container filled with clear water and are kept still for 40min, the fertilized eggs are washed with clear water for 1-2 times after the fertilized eggs fully absorb water and swell the egg membranes and are lifted for 30-40 min.
Preferably, in step S1, feeding shrimp, squid, crab and small trash fish alternately, wherein the feeding is performed for 2-3 times per day, the feeding is performed until the feeding of parent fish stops, the daily feeding amount is 1% -1.5% of the parent fish, and the protein content of the feed is 40-50% per day.
Preferably, in step S1, the pool water dissolved oxygen is not less than 8.0mg/l, and the pH value is 6.8-7.2.
Preferably, the oxytocin combination dose used in the step S2 is the dosage of the fish hormone Zhongnan No. 3: 5ml/kg, chorionic gonadotropin: 1500IU/kg, luteinizing hormone releasing hormone analog: 10 μ g/kg, diutanone: 5 mg/kg.
Preferably, the tubificidae is fed in step S5, the feeding is slowly changed to the mixing feeding of the tubificidae and the feed, and finally the artificial feed is fed.
Preferably, the water temperature for artificial induced spawning in the step S2 is controlled at 16-18 ℃, and the induced spawning effect time is 30-32 h.
Compared with the prior art, the invention has the beneficial effects that: the method has the advantages that the hatchability of fertilized eggs of acrossocheilus fasciatus bred by the method is higher, the incidence rate of nutritional diseases is reduced in the breeding process by adding the water quality improver, the survival rate is improved, and the high yield of the acrossocheilus fasciatus is ensured.
Drawings
FIG. 1 is a flow chart of a method for breeding acrossocheilus fasciatus according to example 1 of the present invention.
Detailed Description
The technical solution of the present invention is further described below by means of specific examples.
Example 1:
as shown in fig. 1, the breeding method of acrossocheilus fasciatus of this embodiment specifically includes the following steps:
s1, selecting and strengthening parent fishes, selecting mature individuals of liptophagus argus of 3-4 ages and 50-200g in weight for culturing, and placing the liptophagus argus in a net cage for culturing with the culture density of 10 fish/m3Feeding the shrimps, the squid, the crabs and the small trash fish alternately for 2 times every day, wherein the feeding is performed until the parent fish stops feeding, the daily feeding amount is 1 to 1.5 percent of the weight of the fish, the protein content of the feed is 40 to 50 percent every day, the dendritic seaweed is fed in an auxiliary manner, 5kg is fed every month, and the culture time is 2 to 3 months; in 11 months of the previous year of the breeding year, the male and female parent fishes are put into a spawning pond according to the quantity ratio of 1:1.2, 4-5 fish of the female parent fishes are put into pond water of each cubic meter, the dissolved oxygen in the pond water is not less than 8.0mg/l, and the pH value is 6.8-7.2.
S2, artificially inducing spawning, starting in 5 months every year, inducing spawning of parent fish when the water temperature is 16-25 ℃, injecting an oxytocic drug at the base of pectoral fins, wherein the injection dosage of the male fish is 1/3-1/2 of female fish, placing the parent fish in a temporary rearing pond for shading rearing after the oxytocic drug is injected, controlling the water temperature at 16-18 ℃, and controlling the effect time to be 30 hours; the oxytocic drug combination dose is No. 3 of fish hormone Zhongnan: 5ml/kg, chorionic gonadotropin: 1500IU/kg, luteinizing hormone releasing hormone analog: 10 μ g/kg, diutanone: 5 mg/kg.
S3, artificial insemination, squeezing the roes and the sperms of the male fishes into a wiped white porcelain basin according to a male-female ratio of 1:1-2, fully stirring the roes and the sperms for 1-2 minutes by using feathers, adding a little clear water, stirring the sperms and the sperms for 1-2 minutes, fully mixing the sperms and the sperms, placing the stirred fertilized eggs into a plastic big basin container filled with clear water, standing the fertilized eggs for 40 minutes, lifting an egg membrane for 35 minutes after the fertilized eggs fully absorb water, and then cleaning the fertilized eggs for 2 times by using clear water; when dead eggs and unfertilized eggs are found, the dead eggs and unfertilized eggs are discharged in time, and after the dead eggs and unfertilized eggs are discharged, water is replaced once every 4 hours, so that the hatching water quality is kept clean.
S4, artificial incubation, namely, incubation in running water or still water, putting incubators into an incubation groove, adding water and putting fertilized eggs into the incubators, wherein the putting density of each incubator is 5 grains/cm3Hatching, wherein the temperature of culture water is 18 ℃, and fertilized eggs begin to rupture membranes and come out after 150-hour hatching; putting 3500 fertilized eggs, controlling the indoor temperature at 16-20 ℃, shading, standing and incubating, and changing water for 2 times every morning and evening, wherein the water is changed for 1/2 times.
S5, raising seedlings, namely putting the fries into pond water with the water temperature of 22-25 ℃, the dissolved oxygen of not less than 8mg/l and the pH value of 7, putting 5000 tails of the fries in each cubic meter of pond water, adding a water quality modifier periodically, feeding earthworm paste three times every day in the first week, wherein the feeding amount is 10-15 g/tail each time; feeding powdered crude protein and earthworm slurry with the mass ratio of 1:1-2 and the protein content of 40-50% in the morning and evening respectively after one week, thereby obtaining the young lipper balm. The morbidity of seedling culture is calculated to be 11.2 percent, the hatchability of fertilized eggs is 91.5 percent, and the yield of seedlings is 24 cubic/kg.
Example 2:
the breeding method of acrossocheilus fasciatus of this embodiment is implemented by the following steps based on embodiment 1,
adding a water quality improving agent to improve water quality in step S4 and step S5; the water quality modifier comprises 12 percent of liquorice, 15 percent of photosynthetic bacteria culture solution, 5 percent of molasses alcohol waste liquid, 3 percent of fulvic acid, 2 percent of EM bacteria, 2 percent of potassium trichloride, 25 percent of dyers woad leaf and the balance of water by mass. In step S4, the temperature of the incubation culture water is controlled to be about 18.3 ℃, so as to intervene in the incubation and shelling period of the fertilized eggs, and the morbidity of seedling culture is calculated to be 1.2%, the hatchability of the fertilized eggs is calculated to be 91.8%, and the yield of seedlings is calculated to be 27 cubic/kg, thereby reducing the morbidity.
Other specific steps are carried out with reference to example 1.
Example 3
The breeding method of acrossocheilus fasciatus of this embodiment is implemented by the following steps based on embodiment 1,
adding a water quality improving agent to improve water quality in step S4 and step S5; the water quality modifier comprises 12 percent of liquorice, 15 percent of photosynthetic bacteria culture solution, 5 percent of molasses alcohol waste liquid, 3 percent of fulvic acid, 2 percent of EM bacteria, 2 percent of potassium trichloride, 25 percent of dyers woad leaf and the balance of water by mass. In step S4, the temperature of the incubation water is adjusted to be higher than 24 ℃, so as to calculate that the incidence rate of seedling is 0.8%, the hatchability of the fertilized eggs is 93.6%, the yield of the seedlings is 28 cubic/kg, and the temperature of the incubation water intervenes in the hatching and shelling period of the fertilized eggs, thereby improving the hatchability of the fertilized eggs.
Other embodiments refer to example 1.
Example 4
The breeding method of acrossocheilus fasciatus of this embodiment is implemented by the following steps based on embodiment 1,
adding a water quality improving agent to improve water quality in step S4 and step S5; the water quality modifier comprises 12 percent of liquorice, 15 percent of photosynthetic bacteria culture solution, 5 percent of molasses alcohol waste liquid, 3 percent of fulvic acid, 2 percent of EM bacteria, 2 percent of potassium trichloride, 25 percent of dyers woad leaf and the balance of water by mass. In step S4, the incubation and shelling period of the fertilized eggs is intervened by adjusting the temperature of the incubation and breeding water to be controlled to be about 20 ℃, and the seedling incidence rate is calculated to be 0.9%, the incubation rate of the fertilized eggs is 97.3%, and the yield of the seedlings is 30 cubic/kg, so that the incubation rate of the fertilized eggs is increased, the incidence rate is reduced, and the yield is increased.
Other embodiments refer to example 1.
Comparative example 1:
the temperature of the hatching culture water of the comparative example is controlled to be less than 18 ℃, no water quality modifier is added, and other operation steps refer to example 1.
The breeding method of the above example was compared with the breeding method of the comparative example, and the evaluation was carried out specifically by the disease incidence, hatchability and seedling yield, as shown in table 1.
TABLE 1 evaluation and comparison table of the above examples and comparative examples
From the above table, the hatchability of the acrossocheilus fasciatus obtained by the breeding method of the invention is improved by at least 5%, the morbidity is reduced by nearly 10%, and the yield is improved by at least 20%.
Although the present invention has been described in detail with reference to the above embodiments, the present invention is not limited to the above embodiments, and may include other equivalent embodiments without departing from the spirit of the present invention, and the scope of the present invention is determined by the scope of the appended claims.
Claims (10)
1. A method for breeding acrossocheilus fasciatus is characterized by comprising the following specific steps:
s1, selecting and strengthening parent fishes, namely, putting the liptophus fasciatus with the weight of 50-200g into a net cage for culture, putting the male and female parent fishes into a spawning pond according to the quantity ratio of 1:1.2, and putting 4-5 tails of the female and male parent fishes in each cubic meter of pond water;
s2, artificially inducing spawning, injecting an oxytocic into the parent fish for inducing spawning, wherein the injection dosage of the male fish is 1/3-1/2 of that of the female fish, and placing the parent fish in a temporary rearing pond for shading and rearing after injecting the oxytocic;
s3, artificial insemination, namely, fully mixing the fish eggs and the male fish semen according to the male-female ratio of 1:1-2 to form fertilized eggs;
s4, artificial incubation, namely incubation in still water, putting the fertilized eggs into incubators filled with culture water, and putting the fertilized eggs into each incubator with the density of 3-6 eggs/cm3Hatching is carried out, shading and standing hatching are carried out for 150-160h, the hatching and shelling period of the fertilized eggs is intervened by adjusting the temperature of culture water, water is changed for 1 time in the morning and at the evening every day, water is changed for 1/2 times, and meanwhile, a water quality modifier is added to improve the water quality;
s5, raising seedlings, namely breeding the hatched fries in pond water, breeding 5000-6000 fries of the fries in each cubic meter of pond water, wherein the dissolved oxygen is not less than 8mg/l, the pH value is 6.8-7.2, and adding a water quality modifier.
2. The breeding method of acrossocheilus fasciatus as claimed in claim 1, wherein the water quality modifier comprises 10% -12% of liquorice, 15% of photosynthetic bacteria culture solution, 5% of molasses alcohol waste liquid, 3% of fulvic acid, 2% of EM bacteria, 2% of potassium trichloride, 20% -30% of folium isatidis and the balance of water by mass.
3. The method for breeding acrossocheilus fasciatus as claimed in claim 1, wherein the temperature of the culture water in step S4 is controlled to 18-24 ℃.
4. The method for breeding acrossocheilus fasciatus as claimed in claim 1, wherein the dead eggs and unfertilized eggs are timely discharged in step S3, and the water is changed every 3-5 hours after the dead eggs and unfertilized eggs are discharged, so as to keep the hatching water clean.
5. The method for breeding lipped fries according to claim 1, wherein in step S3, the fish eggs and the sperms of the male fries are squeezed into a wiped white porcelain basin and stirred, the stirred fertilized eggs are placed in a plastic large basin container filled with clear water and are kept still for 40min, the fertilized eggs are cleaned with clear water for 1-2 times after the fertilized eggs fully absorb water and swell the egg membranes and are lifted for 30-40 min.
6. The method for breeding acrossocheilus fasciatus as claimed in claim 1, wherein the shrimp, squid, crab and small trash fish are fed alternately in step S1, the feeding is performed 2-3 times a day, the feeding is performed until the feeding of parent fish stops, the daily feeding amount is 1% -1.5% of the parent fish, and the protein content of the feed fed every day is 40-50%.
7. The method of claim 1, wherein in step S1, the pond water has a dissolved oxygen content of not less than 8.0mg/l and a pH of 6.8-7.2.
8. The method for breeding lipped fishes as claimed in claim 1, wherein the oxytocin combination dose used in the S2 is the fish hormone Nam No. 3: 5ml/kg, chorionic gonadotropin: 1500IU/kg, luteinizing hormone releasing hormone analog: 10 μ g/kg, diutanone: 5 mg/kg.
9. The method for breeding acrossocheilus fasciatus as claimed in claim 1, wherein the step S5 comprises feeding tubificidae, slowly changing to the step of feeding tubificidae and fodder in a mixed manner, and finally feeding artificial fodder.
10. The method for breeding acrossocheilus fasciatus as claimed in claim 1, wherein the water temperature for artificial induced spawning in step S2 is controlled at 16-18 ℃, and the induced spawning effect time is 30-32 h.
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| CN117296756A (en) * | 2023-10-31 | 2023-12-29 | 珠江水资源保护科学研究所 | Artificial propagation method for Alcheilus longifolia |
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| CN114885867A (en) * | 2022-05-26 | 2022-08-12 | 黄山跃腾渔业有限责任公司 | Breeding and domesticating method for acrossocheilus fasciatus |
| CN117296756A (en) * | 2023-10-31 | 2023-12-29 | 珠江水资源保护科学研究所 | Artificial propagation method for Alcheilus longifolia |
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