CN102574026B - 分离系统和方法 - Google Patents

分离系统和方法 Download PDF

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Publication number
CN102574026B
CN102574026B CN201080043193.9A CN201080043193A CN102574026B CN 102574026 B CN102574026 B CN 102574026B CN 201080043193 A CN201080043193 A CN 201080043193A CN 102574026 B CN102574026 B CN 102574026B
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Prior art keywords
separation
column
line
separation system
pump
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Chinese (zh)
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CN102574026A (zh
Inventor
A·M·威廉斯
A·卡莱特斯基
L·斯塔克
D·G·戈
J·克拉斯南斯基
D·A·瓦雷海姆
B·林
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Globegroup Life Technology Consulting America Co ltd
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GE Healthcare Bio Sciences Corp
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
    • B01D15/18—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns
    • B01D15/1864—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns using two or more columns
    • B01D15/1871—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns using two or more columns placed in series
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/26—Conditioning of the fluid carrier; Flow patterns
    • G01N30/28—Control of physical parameters of the fluid carrier
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/26—Conditioning of the fluid carrier; Flow patterns
    • G01N30/38—Flow patterns
    • G01N30/46—Flow patterns using more than one column
    • G01N30/461—Flow patterns using more than one column with serial coupling of separation columns
    • G01N30/465—Flow patterns using more than one column with serial coupling of separation columns with specially adapted interfaces between the columns
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
    • B01D15/18—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns
    • B01D15/1864—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns using two or more columns
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
    • B01D15/361—Ion-exchange
    • B01D15/362—Cation-exchange
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/04—Preparation or injection of sample to be analysed
    • G01N30/24—Automatic injection systems
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/26—Conditioning of the fluid carrier; Flow patterns
    • G01N30/28—Control of physical parameters of the fluid carrier
    • G01N30/34—Control of physical parameters of the fluid carrier of fluid composition, e.g. gradient

Landscapes

  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Peptides Or Proteins (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Separation Using Semi-Permeable Membranes (AREA)
CN201080043193.9A 2009-09-25 2010-09-20 分离系统和方法 Active CN102574026B (zh)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US12/566,701 2009-09-25
US12/566701 2009-09-25
US12/566,701 US9527010B2 (en) 2009-09-25 2009-09-25 Separation system and method
PCT/SE2010/051003 WO2011037522A1 (en) 2009-09-25 2010-09-20 Separation system and method

Publications (2)

Publication Number Publication Date
CN102574026A CN102574026A (zh) 2012-07-11
CN102574026B true CN102574026B (zh) 2015-10-07

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ID=43779133

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201080043193.9A Active CN102574026B (zh) 2009-09-25 2010-09-20 分离系统和方法

Country Status (7)

Country Link
US (1) US9527010B2 (enExample)
EP (1) EP2480305B1 (enExample)
JP (1) JP5749270B2 (enExample)
CN (1) CN102574026B (enExample)
AU (1) AU2010298768B2 (enExample)
IN (1) IN2012DN01850A (enExample)
WO (1) WO2011037522A1 (enExample)

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CN109453545A (zh) * 2018-12-20 2019-03-12 上海兆维科技发展有限公司 层析柱洗脱液的配液装置
US10429359B2 (en) 2014-02-14 2019-10-01 Ge Healthcare Bio-Sciences Ab Automated multi-step purification system
CN110325853A (zh) * 2016-12-29 2019-10-11 通用电气健康护理生物科学股份公司 生物过程纯化系统和方法

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CN110325853A (zh) * 2016-12-29 2019-10-11 通用电气健康护理生物科学股份公司 生物过程纯化系统和方法
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Also Published As

Publication number Publication date
EP2480305B1 (en) 2020-10-28
US9527010B2 (en) 2016-12-27
US20110073548A1 (en) 2011-03-31
JP2013506127A (ja) 2013-02-21
AU2010298768B2 (en) 2015-07-02
WO2011037522A1 (en) 2011-03-31
EP2480305A4 (en) 2014-07-02
EP2480305A1 (en) 2012-08-01
AU2010298768A1 (en) 2012-03-15
IN2012DN01850A (enExample) 2015-08-21
CN102574026A (zh) 2012-07-11
JP5749270B2 (ja) 2015-07-15

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Patentee before: GE HEALTHCARE BIO-SCIENCES Corp.