JP5749270B2 - 分離システム及び方法 - Google Patents
分離システム及び方法 Download PDFInfo
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- JP5749270B2 JP5749270B2 JP2012530844A JP2012530844A JP5749270B2 JP 5749270 B2 JP5749270 B2 JP 5749270B2 JP 2012530844 A JP2012530844 A JP 2012530844A JP 2012530844 A JP2012530844 A JP 2012530844A JP 5749270 B2 JP5749270 B2 JP 5749270B2
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- separation
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- separation unit
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- 238000000926 separation method Methods 0.000 title claims description 90
- 238000000034 method Methods 0.000 title claims description 41
- 238000004587 chromatography analysis Methods 0.000 claims description 35
- 102000004169 proteins and genes Human genes 0.000 claims description 30
- 108090000623 proteins and genes Proteins 0.000 claims description 30
- 239000012530 fluid Substances 0.000 claims description 23
- 238000000746 purification Methods 0.000 claims description 22
- 241000700605 Viruses Species 0.000 claims description 14
- 239000007788 liquid Substances 0.000 claims description 11
- 238000011068 loading method Methods 0.000 claims description 11
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- 239000013626 chemical specie Substances 0.000 claims description 6
- 238000005349 anion exchange Methods 0.000 claims description 4
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- 238000007405 data analysis Methods 0.000 claims description 4
- 238000001742 protein purification Methods 0.000 claims description 2
- 238000003672 processing method Methods 0.000 claims 1
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- 238000001042 affinity chromatography Methods 0.000 description 3
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- 238000009509 drug development Methods 0.000 description 1
- 238000004128 high performance liquid chromatography Methods 0.000 description 1
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Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
- B01D15/18—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns
- B01D15/1864—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns using two or more columns
- B01D15/1871—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns using two or more columns placed in series
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/26—Conditioning of the fluid carrier; Flow patterns
- G01N30/28—Control of physical parameters of the fluid carrier
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/26—Conditioning of the fluid carrier; Flow patterns
- G01N30/38—Flow patterns
- G01N30/46—Flow patterns using more than one column
- G01N30/461—Flow patterns using more than one column with serial coupling of separation columns
- G01N30/465—Flow patterns using more than one column with serial coupling of separation columns with specially adapted interfaces between the columns
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
- B01D15/18—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns
- B01D15/1864—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns using two or more columns
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
- B01D15/361—Ion-exchange
- B01D15/362—Cation-exchange
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/24—Automatic injection systems
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/26—Conditioning of the fluid carrier; Flow patterns
- G01N30/28—Control of physical parameters of the fluid carrier
- G01N30/34—Control of physical parameters of the fluid carrier of fluid composition, e.g. gradient
Landscapes
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Peptides Or Proteins (AREA)
- Treatment Of Liquids With Adsorbents In General (AREA)
- Separation Using Semi-Permeable Membranes (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US12/566,701 US9527010B2 (en) | 2009-09-25 | 2009-09-25 | Separation system and method |
| US12/566,701 | 2009-09-25 | ||
| PCT/SE2010/051003 WO2011037522A1 (en) | 2009-09-25 | 2010-09-20 | Separation system and method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2013506127A JP2013506127A (ja) | 2013-02-21 |
| JP5749270B2 true JP5749270B2 (ja) | 2015-07-15 |
Family
ID=43779133
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2012530844A Active JP5749270B2 (ja) | 2009-09-25 | 2010-09-20 | 分離システム及び方法 |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US9527010B2 (enExample) |
| EP (1) | EP2480305B1 (enExample) |
| JP (1) | JP5749270B2 (enExample) |
| CN (1) | CN102574026B (enExample) |
| AU (1) | AU2010298768B2 (enExample) |
| IN (1) | IN2012DN01850A (enExample) |
| WO (1) | WO2011037522A1 (enExample) |
Families Citing this family (49)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2480943B1 (en) * | 2009-09-25 | 2017-07-12 | GE Healthcare Bio-Sciences AB | Method and system for preparation of liquid mixtures |
| AU2011214361C1 (en) * | 2010-02-12 | 2016-01-14 | Dpx Holdings B.V. | Single unit antibody purification |
| ES2603396T3 (es) * | 2010-07-30 | 2017-02-27 | Pfizer Inc | Purificación en tándem de proteínas |
| KR20120030971A (ko) * | 2010-09-20 | 2012-03-29 | 아사히 카세이 바이오프로세스 인코포레이티드 | 단백질 분리 및 정제를 위한 액체 크로마토그래피 시스템 및 방법 |
| WO2012059308A1 (en) * | 2010-11-01 | 2012-05-10 | Dsm Ip Assets B.V. | Single unit ion exchange chromatography antibody purification |
| KR20140033126A (ko) * | 2011-06-16 | 2014-03-17 | 디에스엠 아이피 어셋츠 비.브이. | 단일 유닛 크로마토그래피 항체 정제 |
| US9096648B2 (en) * | 2011-08-19 | 2015-08-04 | Emd Millipore Corporation | Methods of reducing level of one or more impurities in a sample during protein purification |
| EP2578286A1 (en) | 2011-10-04 | 2013-04-10 | Merck Patent GmbH | Method and apparatus for chromatographic purification |
| ES2985370T3 (es) * | 2011-11-10 | 2024-11-05 | Hoffmann La Roche | Sistema de cromatografía en columna preparativa |
| WO2013075740A1 (en) | 2011-11-23 | 2013-05-30 | Sanofi | Antibody purification method |
| CN104411820B (zh) * | 2012-06-29 | 2017-05-03 | Emd密理博公司 | 在蛋白纯化过程中灭活病毒的方法 |
| TWI631132B (zh) * | 2013-05-06 | 2018-08-01 | 賽諾菲公司 | 用於純化抗體之連續多步驟方法 |
| CN104237418B (zh) * | 2013-09-11 | 2015-11-18 | 青岛普仁仪器有限公司 | 免稀释作线性曲线的色谱仪及线性曲线测量方法 |
| JPWO2015041218A1 (ja) * | 2013-09-17 | 2017-03-02 | 株式会社カネカ | 新規抗体精製方法及びそれから得られる抗体（ＮｏｖｅｌＡｎｔｉｂｏｄｙＰｕｒｉｆｉｃａｔｉｏｎＭｅｔｈｏｄａｎｄＡｎｔｉｂｏｄｙｏｂｔａｉｎｅｄｔｈｅｒｅｆｒｏｍ）、並びに陽イオン交換基を用いた新規抗体精製法及びそれから得られる抗体（ＮｏｖｅｌＡｎｔｉｂｏｄｙＰｕｒｉｆｉｃａｔｉｏｎｍｅｔｈｏｄｕｓｉｎｇＣａｔｉｏｎＥｘｃｈａｎｇｅｒａｎｄＡｎｔｉｂｏｄｙｏｂｔａｉｎｅｄｔｈｅｒｅｆｒｏｍ） |
| US11286292B2 (en) * | 2014-02-07 | 2022-03-29 | Cmc Biologics A/S | Bioreactor arrangement and continuous process for producing and capturing a biopolymer |
| CN105980847B (zh) * | 2014-02-14 | 2018-12-04 | 通用电气健康护理生物科学股份公司 | 自动多步纯化系统 |
| JP6331485B2 (ja) * | 2014-03-04 | 2018-05-30 | 株式会社島津製作所 | 液体クロマトグラフ及び液体クロマトグラフ分析方法 |
| JP2017515501A (ja) * | 2014-05-02 | 2017-06-15 | ノヴォ ノルディスク アー／エス | 統合型連続バイオマニュファクチャリング方法 |
| US11835501B2 (en) | 2015-07-13 | 2023-12-05 | Sartorius Stedim Chromatography Systems Ltd. | Optimizing operating binding capacity for a multiple column chromatography process |
| JP6579515B2 (ja) * | 2015-08-10 | 2019-09-25 | 国立大学法人 岡山大学 | アポｅ含有ｈｄｌの測定方法及び測定装置 |
| SG10202009671UA (en) * | 2016-04-04 | 2020-11-27 | Boehringer Ingelheim Rcv Gmbh | Real time monitoring of product purification |
| GB201622343D0 (en) * | 2016-12-29 | 2017-02-15 | Ge Healthcare Bio Sciences Ab | Method in bioprocess purification system |
| US11016110B2 (en) | 2017-01-26 | 2021-05-25 | Myriad Women's Health, Inc. | Reagent delivery and waste management system |
| US11185830B2 (en) | 2017-09-06 | 2021-11-30 | Waters Technologies Corporation | Fluid mixer |
| FR3073424B1 (fr) | 2017-11-16 | 2022-03-25 | Novasep Process | Procede regule de separation d’un melange |
| GB201802593D0 (en) * | 2018-02-16 | 2018-04-04 | Fujifilm Diosynth Biotechnologies Uk Ltd | Rig |
| KR102825164B1 (ko) * | 2018-03-27 | 2025-06-26 | 브리스톨-마이어스 스큅 컴퍼니 | 자외선 신호를 사용한 단백질 농도의 실시간 모니터링 |
| EP3546475A1 (en) | 2018-03-27 | 2019-10-02 | Sanofi | Full flow-through process for purifying recombinant proteins |
| CN109453545A (zh) * | 2018-12-20 | 2019-03-12 | 上海兆维科技发展有限公司 | 层析柱洗脱液的配液装置 |
| CA3129330A1 (en) * | 2019-02-11 | 2020-08-20 | Bayer Aktiengesellschaft | Influencing a sequential chromatography in real-time |
| EP3763429A1 (en) * | 2019-07-08 | 2021-01-13 | Bayer Aktiengesellschaft | Influencing a sequential chromatography in real-time |
| EP3693732A1 (en) * | 2019-02-11 | 2020-08-12 | Bayer Aktiengesellschaft | Influencing a sequential chromatography in real-time |
| EP3699697A1 (en) * | 2019-02-25 | 2020-08-26 | Bayer Aktiengesellschaft | Automated simultaneous process control |
| FR3099066B1 (fr) | 2019-07-26 | 2021-08-13 | Novasep Process | Procédé de purification d’une substance cible avec inactivation virale |
| WO2021030245A1 (en) | 2019-08-12 | 2021-02-18 | Waters Technologies Corporation | Mixer for chromatography system |
| GB201911686D0 (en) * | 2019-08-15 | 2019-10-02 | Fujifilm Diosynth Biotechnologies Uk Ltd | Process for purifying target substances |
| GB201911685D0 (en) | 2019-08-15 | 2019-10-02 | Fujifilm Diosynth Biotechnologies Uk Ltd | Process for purifying monoclonal antibodies |
| CA3151967A1 (en) | 2019-09-23 | 2021-04-01 | Marina Hincapie | Product quality attribute measurement |
| JP7388206B2 (ja) * | 2020-01-22 | 2023-11-29 | 株式会社島津製作所 | 液体クロマトグラフおよび分析方法 |
| CN113663364B (zh) * | 2020-05-14 | 2025-02-21 | 佛山汉腾生物科技有限公司 | 层析装置 |
| WO2022010666A1 (en) | 2020-07-07 | 2022-01-13 | Waters Technologies Corporation | Combination mixer arrangement for noise reduction in fluid chromatography |
| CN116134312B (zh) | 2020-07-07 | 2025-12-02 | 沃特世科技公司 | 液相色谱用混合器 |
| TW202227797A (zh) * | 2020-09-17 | 2022-07-16 | 美商計算國際有限公司 | 用於監測元素的存在之系統和方法 |
| CN116194767A (zh) | 2020-09-22 | 2023-05-30 | 沃特世科技公司 | 连续流混合器 |
| JP2022091322A (ja) | 2020-12-09 | 2022-06-21 | 株式会社島津製作所 | 液体クロマトグラフのｐＨ管理システムおよびプログラム |
| CN117355747A (zh) | 2021-05-20 | 2024-01-05 | 沃特世科技公司 | 等分散分流混合器 |
| CN117580636A (zh) | 2021-06-23 | 2024-02-20 | 沃特世科技公司 | 用于液相色谱法的被动溶剂混合器 |
| WO2023165947A1 (en) * | 2022-03-03 | 2023-09-07 | Chromacon Ag | Chromatographic purification method and uses thereof |
| CN114797173A (zh) * | 2022-04-10 | 2022-07-29 | 南通星中细胞工程有限公司 | 一种用于外泌体的高效制备装置及制备方法 |
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2009
- 2009-09-25 US US12/566,701 patent/US9527010B2/en active Active
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2010
- 2010-09-20 EP EP10819116.4A patent/EP2480305B1/en active Active
- 2010-09-20 AU AU2010298768A patent/AU2010298768B2/en active Active
- 2010-09-20 IN IN1850DEN2012 patent/IN2012DN01850A/en unknown
- 2010-09-20 WO PCT/SE2010/051003 patent/WO2011037522A1/en not_active Ceased
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Also Published As
| Publication number | Publication date |
|---|---|
| EP2480305B1 (en) | 2020-10-28 |
| JP2013506127A (ja) | 2013-02-21 |
| AU2010298768A1 (en) | 2012-03-15 |
| CN102574026A (zh) | 2012-07-11 |
| US20110073548A1 (en) | 2011-03-31 |
| AU2010298768B2 (en) | 2015-07-02 |
| CN102574026B (zh) | 2015-10-07 |
| US9527010B2 (en) | 2016-12-27 |
| EP2480305A1 (en) | 2012-08-01 |
| WO2011037522A1 (en) | 2011-03-31 |
| IN2012DN01850A (enExample) | 2015-08-21 |
| EP2480305A4 (en) | 2014-07-02 |
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