JP5749270B2 - 分離システム及び方法 - Google Patents
分離システム及び方法 Download PDFInfo
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- JP5749270B2 JP5749270B2 JP2012530844A JP2012530844A JP5749270B2 JP 5749270 B2 JP5749270 B2 JP 5749270B2 JP 2012530844 A JP2012530844 A JP 2012530844A JP 2012530844 A JP2012530844 A JP 2012530844A JP 5749270 B2 JP5749270 B2 JP 5749270B2
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- separation
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- separation unit
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- 238000000926 separation method Methods 0.000 title claims description 90
- 238000000034 method Methods 0.000 title claims description 41
- 238000004587 chromatography analysis Methods 0.000 claims description 35
- 102000004169 proteins and genes Human genes 0.000 claims description 30
- 108090000623 proteins and genes Proteins 0.000 claims description 30
- 239000012530 fluid Substances 0.000 claims description 23
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- 238000005349 anion exchange Methods 0.000 claims description 4
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- 238000007405 data analysis Methods 0.000 claims description 4
- 238000001742 protein purification Methods 0.000 claims description 2
- 238000003672 processing method Methods 0.000 claims 1
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Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
- B01D15/18—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns
- B01D15/1864—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns using two or more columns
- B01D15/1871—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns using two or more columns placed in series
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/26—Conditioning of the fluid carrier; Flow patterns
- G01N30/28—Control of physical parameters of the fluid carrier
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/26—Conditioning of the fluid carrier; Flow patterns
- G01N30/38—Flow patterns
- G01N30/46—Flow patterns using more than one column
- G01N30/461—Flow patterns using more than one column with serial coupling of separation columns
- G01N30/465—Flow patterns using more than one column with serial coupling of separation columns with specially adapted interfaces between the columns
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
- B01D15/18—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns
- B01D15/1864—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns using two or more columns
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
- B01D15/361—Ion-exchange
- B01D15/362—Cation-exchange
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/24—Automatic injection systems
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/26—Conditioning of the fluid carrier; Flow patterns
- G01N30/28—Control of physical parameters of the fluid carrier
- G01N30/34—Control of physical parameters of the fluid carrier of fluid composition, e.g. gradient
Landscapes
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Peptides Or Proteins (AREA)
- Treatment Of Liquids With Adsorbents In General (AREA)
- Separation Using Semi-Permeable Membranes (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US12/566,701 US9527010B2 (en) | 2009-09-25 | 2009-09-25 | Separation system and method |
| US12/566,701 | 2009-09-25 | ||
| PCT/SE2010/051003 WO2011037522A1 (en) | 2009-09-25 | 2010-09-20 | Separation system and method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2013506127A JP2013506127A (ja) | 2013-02-21 |
| JP5749270B2 true JP5749270B2 (ja) | 2015-07-15 |
Family
ID=43779133
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2012530844A Active JP5749270B2 (ja) | 2009-09-25 | 2010-09-20 | 分離システム及び方法 |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US9527010B2 (enExample) |
| EP (1) | EP2480305B1 (enExample) |
| JP (1) | JP5749270B2 (enExample) |
| CN (1) | CN102574026B (enExample) |
| AU (1) | AU2010298768B2 (enExample) |
| IN (1) | IN2012DN01850A (enExample) |
| WO (1) | WO2011037522A1 (enExample) |
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| CN107983261A (zh) * | 2009-09-25 | 2018-05-04 | 通用电气健康护理生物科学股份公司 | 用于液体混合物的制备的方法和系统 |
| EA201201132A1 (ru) * | 2010-02-12 | 2013-03-29 | ДСМ АйПи АССЕТС Б.В. | Очистка антител с помощью единого функционального блока |
| EP2598515B1 (en) * | 2010-07-30 | 2016-09-21 | Pfizer Inc | Tandem purification of proteins |
| US20130109102A1 (en) * | 2010-09-20 | 2013-05-02 | Hiroyuki Yabe | Liquid chromatography system and method for protein separation and purification |
| AU2011325341B2 (en) * | 2010-11-01 | 2015-12-17 | Dpx Holdings B.V. | Single unit ion exchange chromatography antibody purification |
| EA201400024A1 (ru) * | 2011-06-16 | 2014-04-30 | ДСМ АйПи АССЕТС Б.В. | Хроматографическая очистка антитела в одном устройстве |
| WO2013028330A2 (en) * | 2011-08-19 | 2013-02-28 | Emd Millipore Corporation | Methods of reducing level of one of more impurities in a sample during protein purification |
| EP2578286A1 (en) | 2011-10-04 | 2013-04-10 | Merck Patent GmbH | Method and apparatus for chromatographic purification |
| CA2850581A1 (en) * | 2011-11-10 | 2013-05-16 | F. Hoffmann-La Roche Ag | Preparative column chromatography system |
| WO2013075740A1 (en) | 2011-11-23 | 2013-05-30 | Sanofi | Antibody purification method |
| CN104411820B (zh) | 2012-06-29 | 2017-05-03 | Emd密理博公司 | 在蛋白纯化过程中灭活病毒的方法 |
| HUE038741T2 (hu) * | 2013-05-06 | 2018-11-28 | Sanofi Sa | Folyamatos többlépéses eljárás antitestek tisztítására |
| CN103439433B (zh) * | 2013-09-11 | 2015-06-10 | 青岛普仁仪器有限公司 | 免稀释作线性曲线的色谱仪及线性曲线测量方法 |
| EP3048109A4 (en) * | 2013-09-17 | 2017-04-19 | Kaneka Corporation | Novel antibody purification method and antibody obtained therefrom, and novel antibody purification method using cation exchanger and antibody obtained therefrom |
| WO2015117883A1 (en) * | 2014-02-07 | 2015-08-13 | Cmc Biologics A/S | A bioreactor arrangement and continuous process for producing and capturing a biopolymer |
| CN105980847B (zh) * | 2014-02-14 | 2018-12-04 | 通用电气健康护理生物科学股份公司 | 自动多步纯化系统 |
| JP6331485B2 (ja) * | 2014-03-04 | 2018-05-30 | 株式会社島津製作所 | 液体クロマトグラフ及び液体クロマトグラフ分析方法 |
| CN106661083A (zh) * | 2014-05-02 | 2017-05-10 | 诺和诺德股份有限公司 | 集成的连续生物制造处理方法 |
| US11835501B2 (en) | 2015-07-13 | 2023-12-05 | Sartorius Stedim Chromatography Systems Ltd. | Optimizing operating binding capacity for a multiple column chromatography process |
| JP6579515B2 (ja) * | 2015-08-10 | 2019-09-25 | 国立大学法人 岡山大学 | アポｅ含有ｈｄｌの測定方法及び測定装置 |
| EP3440518B1 (en) * | 2016-04-04 | 2025-11-05 | Boehringer Ingelheim RCV GmbH & Co KG | Real time monitoring of product purification |
| GB201622343D0 (en) * | 2016-12-29 | 2017-02-15 | Ge Healthcare Bio Sciences Ab | Method in bioprocess purification system |
| WO2018140485A1 (en) | 2017-01-26 | 2018-08-02 | Counsyl, Inc. | Reagent delivery and waste management system |
| US11185830B2 (en) | 2017-09-06 | 2021-11-30 | Waters Technologies Corporation | Fluid mixer |
| FR3073424B1 (fr) * | 2017-11-16 | 2022-03-25 | Novasep Process | Procede regule de separation d’un melange |
| GB201802593D0 (en) * | 2018-02-16 | 2018-04-04 | Fujifilm Diosynth Biotechnologies Uk Ltd | Rig |
| US20210024873A1 (en) * | 2018-03-27 | 2021-01-28 | Bristol-Myers Squibb Company | Real-time monitoring of protein concentration using ultraviolet signal |
| EP3546475A1 (en) | 2018-03-27 | 2019-10-02 | Sanofi | Full flow-through process for purifying recombinant proteins |
| CN109453545A (zh) * | 2018-12-20 | 2019-03-12 | 上海兆维科技发展有限公司 | 层析柱洗脱液的配液装置 |
| EP3763429A1 (en) * | 2019-07-08 | 2021-01-13 | Bayer Aktiengesellschaft | Influencing a sequential chromatography in real-time |
| EP3693732A1 (en) * | 2019-02-11 | 2020-08-12 | Bayer Aktiengesellschaft | Influencing a sequential chromatography in real-time |
| BR112021013056A2 (pt) * | 2019-02-11 | 2021-11-23 | Bayer Ag | Influência de uma cromatografia sequencial em tempo real |
| EP3699697A1 (en) * | 2019-02-25 | 2020-08-26 | Bayer Aktiengesellschaft | Automated simultaneous process control |
| FR3099066B1 (fr) | 2019-07-26 | 2021-08-13 | Novasep Process | Procédé de purification d’une substance cible avec inactivation virale |
| CN114207433B (zh) | 2019-08-12 | 2025-01-14 | 沃特世科技公司 | 用于色谱系统的混合器 |
| GB201911685D0 (en) * | 2019-08-15 | 2019-10-02 | Fujifilm Diosynth Biotechnologies Uk Ltd | Process for purifying monoclonal antibodies |
| GB201911686D0 (en) | 2019-08-15 | 2019-10-02 | Fujifilm Diosynth Biotechnologies Uk Ltd | Process for purifying target substances |
| TW202532844A (zh) * | 2019-09-23 | 2025-08-16 | 美商健臻公司 | 用於測量生物樣品的分析物的多個產品品質屬性的方法 |
| JP7388206B2 (ja) * | 2020-01-22 | 2023-11-29 | 株式会社島津製作所 | 液体クロマトグラフおよび分析方法 |
| CN113663364B (zh) * | 2020-05-14 | 2025-02-21 | 佛山汉腾生物科技有限公司 | 层析装置 |
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| WO2022010665A1 (en) | 2020-07-07 | 2022-01-13 | Waters Technologies Corporation | Mixer for liquid chromatography |
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| JP2022091322A (ja) | 2020-12-09 | 2022-06-21 | 株式会社島津製作所 | 液体クロマトグラフのｐＨ管理システムおよびプログラム |
| CN117355747A (zh) | 2021-05-20 | 2024-01-05 | 沃特世科技公司 | 等分散分流混合器 |
| WO2023165947A1 (en) * | 2022-03-03 | 2023-09-07 | Chromacon Ag | Chromatographic purification method and uses thereof |
| CN114797173A (zh) * | 2022-04-10 | 2022-07-29 | 南通星中细胞工程有限公司 | 一种用于外泌体的高效制备装置及制备方法 |
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| EP2421631B1 (en) | 2009-04-23 | 2021-05-26 | Global Life Sciences Solutions USA LLC | Chromatography system and method with feedback control for variable speed loading |
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| WO2011037522A1 (en) | 2011-03-31 |
| CN102574026A (zh) | 2012-07-11 |
| EP2480305A1 (en) | 2012-08-01 |
| US20110073548A1 (en) | 2011-03-31 |
| EP2480305A4 (en) | 2014-07-02 |
| CN102574026B (zh) | 2015-10-07 |
| AU2010298768A1 (en) | 2012-03-15 |
| AU2010298768B2 (en) | 2015-07-02 |
| JP2013506127A (ja) | 2013-02-21 |
| EP2480305B1 (en) | 2020-10-28 |
| US9527010B2 (en) | 2016-12-27 |
| IN2012DN01850A (enExample) | 2015-08-21 |
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