CN102459603B - 岩藻糖基化缺陷型细胞 - Google Patents

岩藻糖基化缺陷型细胞 Download PDF

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CN102459603B
CN102459603B CN2010800294912A CN201080029491A CN102459603B CN 102459603 B CN102459603 B CN 102459603B CN 2010800294912 A CN2010800294912 A CN 2010800294912A CN 201080029491 A CN201080029491 A CN 201080029491A CN 102459603 B CN102459603 B CN 102459603B
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cells
cell
fucosylation
fucose
glycoprotein
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CN102459603A (zh
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陈刚
D·布拉科夫
J·P·凡德尔
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Regeneron Pharmaceuticals Inc
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12P21/00—Preparation of peptides or proteins
    • C12P21/005—Glycopeptides, glycoproteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2863—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against receptors for growth factors, growth regulators
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2866—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against receptors for cytokines, lymphokines, interferons
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01271—GDP-L-fucose synthase (1.1.1.271)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/40—Immunoglobulins specific features characterized by post-translational modification
    • C07K2317/41—Glycosylation, sialylation, or fucosylation
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts

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  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
CN2010800294912A 2009-06-02 2010-06-01 岩藻糖基化缺陷型细胞 Expired - Fee Related CN102459603B (zh)

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Application Number Priority Date Filing Date Title
US18340009P 2009-06-02 2009-06-02
US61/183,400 2009-06-02
US34885810P 2010-05-27 2010-05-27
US61/348,858 2010-05-27
PCT/US2010/036914 WO2010141478A1 (en) 2009-06-02 2010-06-01 Fucosylation-deficient cells

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CN102459603A CN102459603A (zh) 2012-05-16
CN102459603B true CN102459603B (zh) 2013-11-06

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US (8) US8409838B2 (enExample)
EP (3) EP2808393B1 (enExample)
JP (4) JP5744856B2 (enExample)
KR (2) KR101441437B1 (enExample)
CN (1) CN102459603B (enExample)
AU (1) AU2010256753B2 (enExample)
BR (1) BRPI1010035A2 (enExample)
CA (1) CA2764370C (enExample)
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KR102399005B1 (ko) 2014-03-11 2022-05-17 리제너론 파마슈티칼스 인코포레이티드 항-egfrviii 항체 및 그것의 용도
CN106604930B (zh) * 2014-05-30 2021-10-29 新英格兰生物实验室公司 去糖基化试剂和方法
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CA2764370C (en) 2018-08-21
ES2527173T3 (es) 2015-01-21
US9206455B2 (en) 2015-12-08
PL2808393T3 (pl) 2018-04-30
DK2438171T3 (en) 2015-01-26
ES2661074T3 (es) 2018-03-27
US11560550B2 (en) 2023-01-24
US20170211115A1 (en) 2017-07-27
EP2438171B1 (en) 2014-12-17
WO2010141478A1 (en) 2010-12-09
JP2016104045A (ja) 2016-06-09
US20200080124A1 (en) 2020-03-12
US20160090410A1 (en) 2016-03-31
US8409838B2 (en) 2013-04-02
JP6211642B2 (ja) 2017-10-11
CN102459603A (zh) 2012-05-16
EP2438171A1 (en) 2012-04-11
US20190024129A1 (en) 2019-01-24
JP5744856B2 (ja) 2015-07-08
HUE038596T2 (hu) 2018-10-29
TW201107469A (en) 2011-03-01
IL216624A (en) 2015-02-26
IL216624A0 (en) 2012-02-29
PL3279326T3 (pl) 2021-03-08
EP3279326B1 (en) 2020-10-14
KR101441437B1 (ko) 2014-09-25
DK2808393T3 (en) 2018-03-12
JP2015051021A (ja) 2015-03-19
EP2808393A3 (en) 2015-03-11
AU2010256753B2 (en) 2013-11-28
KR101641526B1 (ko) 2016-07-21
TWI513818B (zh) 2015-12-21
US20100304436A1 (en) 2010-12-02
KR20120029443A (ko) 2012-03-26
US20130164786A1 (en) 2013-06-27
US10006070B2 (en) 2018-06-26
DK3279326T3 (da) 2020-12-21
JP2017006147A (ja) 2017-01-12
US20210254022A1 (en) 2021-08-19
AU2010256753A1 (en) 2012-01-12
CA2764370A1 (en) 2010-12-09
SG176251A1 (en) 2011-12-29
EP2808393B1 (en) 2018-01-10
HK1163745A1 (en) 2012-09-14
JP2012528594A (ja) 2012-11-15
EP3279326A1 (en) 2018-02-07
HK1249547A1 (en) 2018-11-02
US9550823B2 (en) 2017-01-24
US20230295583A1 (en) 2023-09-21
US12054751B2 (en) 2024-08-06
BRPI1010035A2 (pt) 2015-08-25
PL2438171T3 (pl) 2015-04-30
KR20140039340A (ko) 2014-04-01
EP2808393A2 (en) 2014-12-03
HK1198180A1 (en) 2015-03-13

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