JP5744856B2 - フコシル化欠損細胞 - Google Patents

フコシル化欠損細胞 Download PDF

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Publication number
JP5744856B2
JP5744856B2 JP2012514045A JP2012514045A JP5744856B2 JP 5744856 B2 JP5744856 B2 JP 5744856B2 JP 2012514045 A JP2012514045 A JP 2012514045A JP 2012514045 A JP2012514045 A JP 2012514045A JP 5744856 B2 JP5744856 B2 JP 5744856B2
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cell
cells
protein
glycoprotein
fucose
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Expired - Fee Related
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JP2012514045A
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Japanese (ja)
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JP2012528594A (ja
Inventor
ギャング チェン，
ギャング チェン，
ダーリア ブラコフ，
ダーリア ブラコフ，
ジェームス ピー． ファンドル，
ジェームス ピー． ファンドル，
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Regeneron Pharmaceuticals Inc
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Regeneron Pharmaceuticals Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/005—Glycopeptides, glycoproteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2863—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against receptors for growth factors, growth regulators
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2866—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against receptors for cytokines, lymphokines, interferons
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01271—GDP-L-fucose synthase (1.1.1.271)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/40—Immunoglobulins specific features characterized by post-translational modification
    • C07K2317/41—Glycosylation, sialylation, or fucosylation
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
JP2012514045A 2009-06-02 2010-06-01 フコシル化欠損細胞 Expired - Fee Related JP5744856B2 (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US18340009P 2009-06-02 2009-06-02
US61/183,400 2009-06-02
US34885810P 2010-05-27 2010-05-27
US61/348,858 2010-05-27
PCT/US2010/036914 WO2010141478A1 (en) 2009-06-02 2010-06-01 Fucosylation-deficient cells

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2014251541A Division JP2015051021A (ja) 2009-06-02 2014-12-12 フコシル化欠損細胞

Publications (2)

Publication Number Publication Date
JP2012528594A JP2012528594A (ja) 2012-11-15
JP5744856B2 true JP5744856B2 (ja) 2015-07-08

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Family Applications (4)

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JP2012514045A Expired - Fee Related JP5744856B2 (ja) 2009-06-02 2010-06-01 フコシル化欠損細胞
JP2014251541A Withdrawn JP2015051021A (ja) 2009-06-02 2014-12-12 フコシル化欠損細胞
JP2016043151A Expired - Fee Related JP6211642B2 (ja) 2009-06-02 2016-03-07 フコシル化欠損細胞
JP2016202634A Pending JP2017006147A (ja) 2009-06-02 2016-10-14 フコシル化欠損細胞

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JP2014251541A Withdrawn JP2015051021A (ja) 2009-06-02 2014-12-12 フコシル化欠損細胞
JP2016043151A Expired - Fee Related JP6211642B2 (ja) 2009-06-02 2016-03-07 フコシル化欠損細胞
JP2016202634A Pending JP2017006147A (ja) 2009-06-02 2016-10-14 フコシル化欠損細胞

Country Status (16)

Country Link
US (8) US8409838B2 (enExample)
EP (3) EP3279326B1 (enExample)
JP (4) JP5744856B2 (enExample)
KR (2) KR101641526B1 (enExample)
CN (1) CN102459603B (enExample)
AU (1) AU2010256753B2 (enExample)
BR (1) BRPI1010035A2 (enExample)
CA (1) CA2764370C (enExample)
DK (3) DK3279326T3 (enExample)
ES (2) ES2661074T3 (enExample)
HU (1) HUE038596T2 (enExample)
IL (1) IL216624A (enExample)
PL (3) PL2808393T3 (enExample)
SG (1) SG176251A1 (enExample)
TW (1) TWI513818B (enExample)
WO (1) WO2010141478A1 (enExample)

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AU2012226398B9 (en) 2011-03-06 2017-04-13 Merck Serono S.A. Low fucose cell lines and uses thereof
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TW201823460A (zh) 2012-05-29 2018-07-01 美商再生元醫藥公司 生產細胞株增強子
EP2722673B1 (en) 2012-10-17 2017-07-12 Hexal AG Improved method of mapping glycans of glycoproteins
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US20150093782A1 (en) * 2013-10-01 2015-04-02 The University Of Wyoming Compositions and methods for reducing fucosylation of glycoproteins in insect cells and methods of use thereof for production of recombinant glycoproteins
AU2015229591B2 (en) 2014-03-11 2020-10-22 Regeneron Pharmaceuticals, Inc. Anti-EGFRvlll antibodies and uses thereof
CN106604930B (zh) * 2014-05-30 2021-10-29 新英格兰生物实验室公司 去糖基化试剂和方法
CA2954534C (en) * 2014-07-07 2023-04-04 Targazyme, Inc. Manufacture and cryopreservation of fucosylated cells for therapeutic use
CN105671109B (zh) * 2014-11-20 2019-08-23 中国人民解放军军事医学科学院生物工程研究所 用糖基工程酵母制备具有动物细胞糖基化修饰流感血凝素糖蛋白的方法
TWI710573B (zh) 2015-01-26 2020-11-21 美商再生元醫藥公司 抗伊波拉病毒醣蛋白之人類抗體
TW202440903A (zh) 2015-08-04 2024-10-16 美商再生元醫藥公司 補充牛磺酸之細胞培養基及用法（一）
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KR20230017815A (ko) 2020-05-26 2023-02-06 리제너론 파아마슈티컬스, 인크. 항-sars-cov-2 스파이크 당단백질 항체 및 항원-결합 단편
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US20200080124A1 (en) 2020-03-12
DK2808393T3 (en) 2018-03-12
US9206455B2 (en) 2015-12-08
JP2015051021A (ja) 2015-03-19
WO2010141478A1 (en) 2010-12-09
HK1198180A1 (en) 2015-03-13
US20100304436A1 (en) 2010-12-02
TW201107469A (en) 2011-03-01
KR101641526B1 (ko) 2016-07-21
PL2808393T3 (pl) 2018-04-30
US20190024129A1 (en) 2019-01-24
EP2808393A3 (en) 2015-03-11
US10006070B2 (en) 2018-06-26
JP2016104045A (ja) 2016-06-09
US20160090410A1 (en) 2016-03-31
JP2017006147A (ja) 2017-01-12
DK3279326T3 (da) 2020-12-21
EP2808393A2 (en) 2014-12-03
HK1249547A1 (en) 2018-11-02
EP3279326A1 (en) 2018-02-07
PL2438171T3 (pl) 2015-04-30
US12054751B2 (en) 2024-08-06
EP2438171A1 (en) 2012-04-11
KR20120029443A (ko) 2012-03-26
US20170211115A1 (en) 2017-07-27
EP2808393B1 (en) 2018-01-10
ES2527173T3 (es) 2015-01-21
US20130164786A1 (en) 2013-06-27
AU2010256753A1 (en) 2012-01-12
IL216624A0 (en) 2012-02-29
SG176251A1 (en) 2011-12-29
US11560550B2 (en) 2023-01-24
BRPI1010035A2 (pt) 2015-08-25
JP6211642B2 (ja) 2017-10-11
IL216624A (en) 2015-02-26
HUE038596T2 (hu) 2018-10-29
JP2012528594A (ja) 2012-11-15
KR20140039340A (ko) 2014-04-01
US20230295583A1 (en) 2023-09-21
KR101441437B1 (ko) 2014-09-25
US8409838B2 (en) 2013-04-02
AU2010256753B2 (en) 2013-11-28
DK2438171T3 (en) 2015-01-26
CN102459603A (zh) 2012-05-16
HK1163745A1 (en) 2012-09-14
EP2438171B1 (en) 2014-12-17
TWI513818B (zh) 2015-12-21
CA2764370A1 (en) 2010-12-09
EP3279326B1 (en) 2020-10-14
PL3279326T3 (pl) 2021-03-08
US9550823B2 (en) 2017-01-24
CA2764370C (en) 2018-08-21
CN102459603B (zh) 2013-11-06
US20210254022A1 (en) 2021-08-19
ES2661074T3 (es) 2018-03-27

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