CN102428173B - Nk细胞的扩增 - Google Patents

Nk细胞的扩增 Download PDF

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Publication number
CN102428173B
CN102428173B CN201080021237.8A CN201080021237A CN102428173B CN 102428173 B CN102428173 B CN 102428173B CN 201080021237 A CN201080021237 A CN 201080021237A CN 102428173 B CN102428173 B CN 102428173B
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cells
cell
expansion
phenotype
natural killer
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CN102428173A (zh
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E.阿里西
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Avaris AB
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Avaris LLC
Cellprotect Nordic Pharmaceuticals AB
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/38—Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factors; Stimulation of growth by removal of a chemical compound
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0646—Natural killers cells [NK], NKT cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/23—Interleukins [IL]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/23—Interleukins [IL]
    • C12N2501/2302—Interleukin-2 (IL-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/50—Cell markers; Cell surface determinants
    • C12N2501/515—CD3, T-cell receptor complex
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/11—Coculture with; Conditioned medium produced by blood or immune system cells
    • C12N2502/1164—NK cells

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • Cell Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Public Health (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Virology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Veterinary Medicine (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CN201080021237.8A 2009-03-26 2010-03-25 Nk细胞的扩增 Active CN102428173B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US16359009P 2009-03-26 2009-03-26
US61/163,590 2009-03-26
PCT/SE2010/050333 WO2010110734A1 (en) 2009-03-26 2010-03-25 Expansion of nk cells

Publications (2)

Publication Number Publication Date
CN102428173A CN102428173A (zh) 2012-04-25
CN102428173B true CN102428173B (zh) 2014-07-30

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CN201080021237.8A Active CN102428173B (zh) 2009-03-26 2010-03-25 Nk细胞的扩增

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Country Link
US (1) US8877182B2 (enExample)
EP (1) EP2411507B1 (enExample)
JP (3) JP2012521215A (enExample)
KR (1) KR101881520B1 (enExample)
CN (1) CN102428173B (enExample)
DK (1) DK2411507T3 (enExample)
ES (1) ES2763169T3 (enExample)
PL (1) PL2411507T3 (enExample)
PT (1) PT2411507T (enExample)
WO (1) WO2010110734A1 (enExample)

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JP5996533B2 (ja) 2010-07-13 2016-09-21 アントフロゲネシス コーポレーション ナチュラルキラー細胞を生成させる方法
JP5572863B2 (ja) * 2011-06-24 2014-08-20 国立大学法人九州大学 Ｎｋ細胞の増幅方法
JP6010136B2 (ja) * 2011-12-22 2016-10-19 モガム バイオテクノロジー インスティチュート ナチュラルキラー細胞の製造方法、その方法により製造されたナチュラルキラー細胞、並びにそれを含む腫瘍及び感染性疾患治療用組成物
US10066207B2 (en) 2012-04-18 2018-09-04 Board Of Trustees Of Michigan State University Natural killer cells with enhanced immune response
KR101525199B1 (ko) * 2012-09-13 2015-06-04 (주)케이셀바이오 100㎖ 이하 말초혈액으로부터 ｎｋ세포의 대량 증식 방법
CN102994449A (zh) * 2012-12-13 2013-03-27 上海柯莱逊生物技术有限公司 一种体外扩增nk细胞的方法
US9511092B2 (en) 2013-01-28 2016-12-06 St. Jude Children's Research Hospital, Inc. Chimeric receptor with NKG2D specificity for use in cell therapy against cancer and infectious disease
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US20160075996A1 (en) * 2013-03-27 2016-03-17 Biotherapy Institute Of Japan Method for producing nk cell-enriched blood preparation
US10260038B2 (en) 2013-05-10 2019-04-16 Whitehead Institute For Biomedical Research Protein modification of living cells using sortase
WO2015125113A1 (en) * 2014-02-21 2015-08-27 Dr. Reddy's Laboratories Limited Method of culturing high cell density pbmc's
CN105002139B (zh) * 2014-04-25 2019-02-01 中国医学科学院肿瘤医院 一种cd3+cd8+cd56+t细胞亚型促进nk细胞增殖的方法
AU2015259877B2 (en) 2014-05-15 2021-02-25 National University Of Singapore Modified natural killer cells and uses thereof
CN104357390B (zh) * 2014-10-15 2017-07-07 恒瑞源正（深圳）生物科技有限公司 同时扩增cd3+cd56+cik细胞和cd3‑cd56+nk细胞的方法
KR101697473B1 (ko) 2014-11-26 2017-01-18 주식회사 녹십자랩셀 T 세포를 이용한 자연살해세포의 배양방법
JP7572700B2 (ja) * 2015-10-19 2024-10-24 ユニバーシティ オブ メリーランド ボルチモア 改変ヒト初代血液樹状細胞株を生成するための方法
CN105624109B (zh) * 2016-02-26 2019-07-12 广州赛莱拉干细胞科技股份有限公司 一种使用生物反应器培养nkt细胞的方法
CN107779434A (zh) * 2016-08-30 2018-03-09 天津市康婷生物工程有限公司 高效扩增人外周血nk细胞的实验方法
EP3580341A4 (en) 2017-02-09 2020-11-04 Indapta Therapeutics, Inc. Engineered natural killer (nk) cells and compositions and methods thereof
BR112019019917A2 (pt) 2017-03-27 2020-04-22 Nat Univ Singapore receptores quiméricos nkg2d truncados e seus usos em imunoterapia de células exterminadoras naturais
WO2018182511A1 (en) 2017-03-27 2018-10-04 National University Of Singapore Stimulatory cell lines for ex vivo expansion and activation of natural killer cells
AU2018271755B2 (en) 2017-05-26 2021-12-23 Green Cross Lab Cell Corporation Method for culturing natural killer cell, using transformed T cell
CN107541491A (zh) * 2017-10-12 2018-01-05 广州金晟生物科技有限公司 一种nk细胞体外培养方法
KR102265437B1 (ko) 2017-11-24 2021-06-15 의료법인 성광의료재단 Nk 배양용 조성물 및 이를 이용하여 nk 세포를 배양하는 방법
CN108060130B (zh) * 2017-12-29 2018-12-07 广州沙艾生物科技有限公司 控制培养箱适于免疫细胞的诱导扩增的方法
CN108192868B (zh) * 2017-12-29 2018-12-07 广州沙艾生物科技有限公司 免疫细胞的诱导扩增方法
EP3746095A4 (en) 2018-02-01 2021-04-21 Nkmax Co., Ltd. Method of producing natural killer cells and composition for treating cancer
AU2019219454A1 (en) 2018-02-09 2020-08-27 National University Of Singapore Activating chimeric receptors and uses thereof in natural killer cell immunotherapy
ES2973728T3 (es) * 2018-03-13 2024-06-24 Fundacion Para La Investigacion Biomedica Del Hospital Univ La Paz Anticuerpo anti-CXCR4 combinado con linfocitos citolíticos naturales activados y expandidos para inmunoterapia contra el cáncer
KR102232321B1 (ko) * 2018-03-23 2021-03-26 주식회사 녹십자랩셀 자연살해세포의 제조방법
WO2019182392A1 (ko) * 2018-03-23 2019-09-26 주식회사 녹십자랩셀 자연살해세포의 제조방법
SG11202008976YA (en) 2018-04-02 2020-10-29 Nat Univ Singapore Neutralization of human cytokines with membrane-bound anti-cytokine non-signaling binders expressed in immune cells
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KR102069704B1 (ko) * 2018-05-16 2020-01-23 고려대학교 산학협력단 Hdac 억제제를 이용한 사람 유래 자연살해세포의 확장 배양법
EP3822341A4 (en) * 2018-05-31 2022-03-09 Korea University Research and Business Foundation EXPANSION AND CULTURE OF HUMAN-DERIVED NATURAL KILLER CELLS USING IGFBP2
CN110628715B (zh) * 2018-06-21 2022-11-25 精准生技股份有限公司 体外扩增自然杀手细胞及自然杀手t细胞的方法及其医药组成物
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Publication number Publication date
EP2411507A4 (en) 2013-06-19
PL2411507T3 (pl) 2020-07-13
KR20110132618A (ko) 2011-12-08
CN102428173A (zh) 2012-04-25
EP2411507A1 (en) 2012-02-01
JP2016105713A (ja) 2016-06-16
JP6574823B2 (ja) 2019-09-11
DK2411507T3 (da) 2020-01-06
KR101881520B1 (ko) 2018-07-24
WO2010110734A1 (en) 2010-09-30
US8877182B2 (en) 2014-11-04
PT2411507T (pt) 2020-01-09
ES2763169T3 (es) 2020-05-27
EP2411507B1 (en) 2019-09-25
US20120258085A1 (en) 2012-10-11
JP2018046840A (ja) 2018-03-29
JP2012521215A (ja) 2012-09-13
HK1170258A1 (en) 2013-02-22

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