CN102409004A - 组织形成乳酸细菌 - Google Patents
组织形成乳酸细菌 Download PDFInfo
- Publication number
- CN102409004A CN102409004A CN200910224873XA CN200910224873A CN102409004A CN 102409004 A CN102409004 A CN 102409004A CN 200910224873X A CN200910224873X A CN 200910224873XA CN 200910224873 A CN200910224873 A CN 200910224873A CN 102409004 A CN102409004 A CN 102409004A
- Authority
- CN
- China
- Prior art keywords
- seq
- bacterial strain
- milk
- bacterium
- sequence
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 241000894006 Bacteria Species 0.000 title claims abstract description 34
- 238000000034 method Methods 0.000 claims abstract description 22
- 235000013305 food Nutrition 0.000 claims abstract description 15
- 108020004707 nucleic acids Proteins 0.000 claims abstract description 15
- 102000039446 nucleic acids Human genes 0.000 claims abstract description 15
- 150000007523 nucleic acids Chemical class 0.000 claims abstract description 15
- 239000013612 plasmid Substances 0.000 claims abstract description 13
- 235000013336 milk Nutrition 0.000 claims abstract description 11
- 239000008267 milk Substances 0.000 claims abstract description 11
- 210000004080 milk Anatomy 0.000 claims abstract description 11
- 230000001580 bacterial effect Effects 0.000 claims description 120
- 241000194017 Streptococcus Species 0.000 claims description 48
- 235000015140 cultured milk Nutrition 0.000 claims description 33
- 239000000203 mixture Substances 0.000 claims description 24
- 239000002773 nucleotide Substances 0.000 claims description 19
- 125000003729 nucleotide group Chemical group 0.000 claims description 19
- 241000194020 Streptococcus thermophilus Species 0.000 claims description 18
- 241000186660 Lactobacillus Species 0.000 claims description 14
- 229940039696 lactobacillus Drugs 0.000 claims description 14
- 241000194036 Lactococcus Species 0.000 claims description 13
- 241000186000 Bifidobacterium Species 0.000 claims description 12
- 241000192132 Leuconostoc Species 0.000 claims description 12
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 8
- 241000131448 Mycosphaerella Species 0.000 claims description 6
- 241000192001 Pediococcus Species 0.000 claims description 6
- 235000013351 cheese Nutrition 0.000 claims description 6
- 238000013519 translation Methods 0.000 claims description 5
- 239000006071 cream Substances 0.000 claims description 4
- 235000012041 food component Nutrition 0.000 claims description 4
- 239000005417 food ingredient Substances 0.000 claims description 4
- 235000021262 sour milk Nutrition 0.000 claims description 4
- 239000013604 expression vector Substances 0.000 claims description 3
- 239000000463 material Substances 0.000 claims description 3
- 230000000813 microbial effect Effects 0.000 claims description 3
- 239000008194 pharmaceutical composition Substances 0.000 claims description 3
- 239000000126 substance Substances 0.000 claims description 3
- 241001465754 Metazoa Species 0.000 claims description 2
- 235000013361 beverage Nutrition 0.000 claims description 2
- 235000021185 dessert Nutrition 0.000 claims description 2
- 235000013350 formula milk Nutrition 0.000 claims description 2
- 235000015061 fromage frais Nutrition 0.000 claims description 2
- 235000011868 grain product Nutrition 0.000 claims description 2
- 235000013622 meat product Nutrition 0.000 claims description 2
- 235000020124 milk-based beverage Nutrition 0.000 claims description 2
- 239000011148 porous material Substances 0.000 claims description 2
- 230000008521 reorganization Effects 0.000 claims description 2
- 230000001131 transforming effect Effects 0.000 claims description 2
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract description 4
- 238000004519 manufacturing process Methods 0.000 abstract description 3
- 239000013598 vector Substances 0.000 abstract description 3
- LWGJTAZLEJHCPA-UHFFFAOYSA-N n-(2-chloroethyl)-n-nitrosomorpholine-4-carboxamide Chemical compound ClCCN(N=O)C(=O)N1CCOCC1 LWGJTAZLEJHCPA-UHFFFAOYSA-N 0.000 description 49
- 150000004676 glycans Chemical class 0.000 description 35
- 229920001282 polysaccharide Polymers 0.000 description 34
- 239000005017 polysaccharide Substances 0.000 description 34
- 108020004414 DNA Proteins 0.000 description 20
- 108090000623 proteins and genes Proteins 0.000 description 13
- 238000012360 testing method Methods 0.000 description 13
- 238000004458 analytical method Methods 0.000 description 10
- 108700023372 Glycosyltransferases Proteins 0.000 description 7
- 102000051366 Glycosyltransferases Human genes 0.000 description 7
- 238000011160 research Methods 0.000 description 7
- 230000006870 function Effects 0.000 description 6
- 238000003860 storage Methods 0.000 description 6
- 238000000855 fermentation Methods 0.000 description 5
- 230000004151 fermentation Effects 0.000 description 5
- 230000014759 maintenance of location Effects 0.000 description 5
- 230000001953 sensory effect Effects 0.000 description 5
- 229920001817 Agar Polymers 0.000 description 4
- 238000003639 Student–Newman–Keuls (SNK) method Methods 0.000 description 4
- 239000008272 agar Substances 0.000 description 4
- 239000011248 coating agent Substances 0.000 description 4
- 238000000576 coating method Methods 0.000 description 4
- 101150072043 deoD gene Proteins 0.000 description 4
- 238000013461 design Methods 0.000 description 4
- 238000005259 measurement Methods 0.000 description 4
- 238000003752 polymerase chain reaction Methods 0.000 description 4
- 238000006116 polymerization reaction Methods 0.000 description 4
- 229920001184 polypeptide Polymers 0.000 description 4
- 102000004196 processed proteins & peptides Human genes 0.000 description 4
- 102000004169 proteins and genes Human genes 0.000 description 4
- 101150042478 punA gene Proteins 0.000 description 4
- OVSKIKFHRZPJSS-UHFFFAOYSA-N 2,4-D Chemical compound OC(=O)COC1=CC=C(Cl)C=C1Cl OVSKIKFHRZPJSS-UHFFFAOYSA-N 0.000 description 3
- 241001478240 Coccus Species 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 3
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Chemical compound OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 3
- 102000008579 Transposases Human genes 0.000 description 3
- 108010020764 Transposases Proteins 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- 230000015572 biosynthetic process Effects 0.000 description 3
- 210000001772 blood platelet Anatomy 0.000 description 3
- 230000008859 change Effects 0.000 description 3
- 230000002596 correlated effect Effects 0.000 description 3
- 235000013365 dairy product Nutrition 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 230000035807 sensation Effects 0.000 description 3
- 235000019615 sensations Nutrition 0.000 description 3
- 238000003756 stirring Methods 0.000 description 3
- 230000009772 tissue formation Effects 0.000 description 3
- 241000193798 Aerococcus Species 0.000 description 2
- 108010046068 N-Acetyllactosamine Synthase Proteins 0.000 description 2
- 230000003321 amplification Effects 0.000 description 2
- 238000000540 analysis of variance Methods 0.000 description 2
- 238000003556 assay Methods 0.000 description 2
- 239000000969 carrier Substances 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 101150106475 epsD gene Proteins 0.000 description 2
- 238000009920 food preservation Methods 0.000 description 2
- 238000013467 fragmentation Methods 0.000 description 2
- 238000006062 fragmentation reaction Methods 0.000 description 2
- 239000000499 gel Substances 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 238000003780 insertion Methods 0.000 description 2
- 230000037431 insertion Effects 0.000 description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
- 238000003199 nucleic acid amplification method Methods 0.000 description 2
- 230000001105 regulatory effect Effects 0.000 description 2
- 238000000518 rheometry Methods 0.000 description 2
- 230000002103 transcriptional effect Effects 0.000 description 2
- 241001112741 Bacillaceae Species 0.000 description 1
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- 241000588807 Bordetella Species 0.000 description 1
- 241000283707 Capra Species 0.000 description 1
- 241000206594 Carnobacterium Species 0.000 description 1
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 1
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 1
- 241000194033 Enterococcus Species 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 101100022281 Escherichia coli O157:H7 manC1 gene Proteins 0.000 description 1
- 241000192125 Firmicutes Species 0.000 description 1
- 244000068988 Glycine max Species 0.000 description 1
- 235000010469 Glycine max Nutrition 0.000 description 1
- 241000202223 Oenococcus Species 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 108020004511 Recombinant DNA Proteins 0.000 description 1
- 101100005430 Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) cpsA1 gene Proteins 0.000 description 1
- 238000012300 Sequence Analysis Methods 0.000 description 1
- 108091081024 Start codon Proteins 0.000 description 1
- 108020005038 Terminator Codon Proteins 0.000 description 1
- 241001537924 Tetracoccus <angiosperm> Species 0.000 description 1
- 241000500334 Tetragenococcus Species 0.000 description 1
- 241000207194 Vagococcus Species 0.000 description 1
- 241000202221 Weissella Species 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000000692 anti-sense effect Effects 0.000 description 1
- 238000003491 array Methods 0.000 description 1
- 230000037396 body weight Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 101150002095 capB gene Proteins 0.000 description 1
- 101150055997 capD gene Proteins 0.000 description 1
- 238000010367 cloning Methods 0.000 description 1
- 230000001332 colony forming effect Effects 0.000 description 1
- 230000000295 complement effect Effects 0.000 description 1
- 101150027335 cpsB gene Proteins 0.000 description 1
- 101150091586 cpsC gene Proteins 0.000 description 1
- 101150030402 cpsD gene Proteins 0.000 description 1
- 101150094781 cpsE gene Proteins 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000004520 electroporation Methods 0.000 description 1
- 101150055072 epsA gene Proteins 0.000 description 1
- 101150107965 epsB gene Proteins 0.000 description 1
- 101150045989 epsC gene Proteins 0.000 description 1
- 101150043985 epsE gene Proteins 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 125000003147 glycosyl group Chemical group 0.000 description 1
- 238000009396 hybridization Methods 0.000 description 1
- 238000009776 industrial production Methods 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 239000004310 lactic acid Substances 0.000 description 1
- 235000014655 lactic acid Nutrition 0.000 description 1
- 101150032120 manC gene Proteins 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 108020004999 messenger RNA Proteins 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 230000020477 pH reduction Effects 0.000 description 1
- 238000009928 pasteurization Methods 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 239000006041 probiotic Substances 0.000 description 1
- 230000000529 probiotic effect Effects 0.000 description 1
- 235000018291 probiotics Nutrition 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 230000000630 rising effect Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 235000020183 skimmed milk Nutrition 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 238000012916 structural analysis Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 238000012549 training Methods 0.000 description 1
- 230000032895 transmembrane transport Effects 0.000 description 1
- 238000007492 two-way ANOVA Methods 0.000 description 1
- 241001515965 unidentified phage Species 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
- A23C9/00—Milk preparations; Milk powder or milk powder preparations
- A23C9/12—Fermented milk preparations; Treatment using microorganisms or enzymes
- A23C9/123—Fermented milk preparations; Treatment using microorganisms or enzymes using only microorganisms of the genus lactobacteriaceae; Yoghurt
- A23C9/1238—Fermented milk preparations; Treatment using microorganisms or enzymes using only microorganisms of the genus lactobacteriaceae; Yoghurt using specific L. bulgaricus or S. thermophilus microorganisms; using entrapped or encapsulated yoghurt bacteria; Physical or chemical treatment of L. bulgaricus or S. thermophilus cultures; Fermentation only with L. bulgaricus or only with S. thermophilus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/21—Streptococcus, lactococcus
- A23V2400/249—Thermophilus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/46—Streptococcus ; Enterococcus; Lactococcus
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Polymers & Plastics (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Food Science & Technology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Dairy Products (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
本发明涉及一种新的乳酸细菌菌株，其包含选自由核苷酸序列SEQID No.1，SEQ ID No.2，SEQ ID No.3，SEQ ID No.4，SEQ ID No.5，SEQID No.6，SEQ ID No.7，SEQ ID No.8组成的组的至少一个序列，以及涉及生产所述菌株的方法。本发明也涉及SEQ ID Nos.1-8的序列以及包含所述序列的核酸、载体和质粒。本发明还涉及食品，特别是含有所述菌株的奶制品。
Description
本申请是200480007243.2(PCT/FR2004/000610)的分案申请。
原案申请日：2004年03月12日
原案申请号：200480007243.2
原申请名称：组织形成乳酸细菌
技术领域
本发明的主题是包含选自由核苷酸序列SEQ ID No.1、SEQ ID No.2、SEQ ID No.3、SEQ ID No.4、SEQ ID No.5、SEQ ID No.6、SEQ ID No.7、SEQ ID No.8组成的组的至少一个序列的乳酸细菌菌株，以及构建这些菌株的方法。最后，本发明涉及包含所述菌株和/或由所述菌株生产的食品。
背景技术
食品工业使用很多细菌，尤其是在发酵形式下，尤其是乳酸细菌，为了改善食品味道和质地，和为了延长这些食品的贮存期限。在乳制品工业中，充分使用乳酸细菌，为了致使牛奶酸化(通过发酵)，和为了使掺入它们的产品达到一定稠度。
在食品工业中使用的乳酸细菌当中，可以提及链球菌属(Streptococcus)、乳球菌属(Lactococcus)、乳杆菌属(Lactobacillus)、明串珠菌属(Leuconostoc)、小球菌属(Pediococcus)和双歧杆菌属(Bifidobacterium)。
嗜热链球菌(Streptococcus thermophilus)种的乳酸细菌被广泛地单独或与其它细菌联合使用生产食品，尤其是发酵产品。它们尤其用于用来生产发酵乳的发酵制剂中，例如酸奶。它们中的某些在发酵产品的质地发展中起着主要作用。这个特征与多糖的生产息息相关。在嗜热链球菌菌株当中，有可能区分组织形成和非组织形成菌株。
嗜热链球菌的组织形成菌株意思是在如实施例所述条件下，产生粘度大于大约35Pa.s、触变域小于大约2000Pa/s和屈服点小于大约14Pa的发酵乳的菌株。嗜热链球菌菌株可以定义为强烈组织形成，因为在如实施例所述条件下，它产生粘度大于大约50Pa.s、触变域小于大约1000Pa/s和屈服点小于大约10Pa的发酵乳。
发明内容
为了满足工业需要，提出乳酸细菌，尤其是嗜热链球菌的新的组织形成菌株变得必需。
因此，本发明提出解决的问题是提供具有好的组织形成食品性能的乳酸细菌菌株。
为此目的，本发明提出了包含选自由核苷酸序列SEQ ID No.1、SEQ IDNo.2、SEQ ID No.3、SEQ ID No.4、SEQ ID No.5、SEQ ID No.6、SEQ IDNo.7、SEQ ID No.8组成的组的至少一个序列的乳酸细菌菌株。
本发明也提出了2003年2月26日保藏在国立微生物保藏中心，保藏号为I-2980的新的嗜热链球菌菌株。
核苷酸序列SEQ ID No.1、SEQ ID No.2、SEQ ID No.3、SEQ ID No.4、SEQ ID No.5、SEQ ID No.6、SEQ ID No.7、SEQ ID No.8，以及包含这些核苷酸序列中至少一个的核酸也是本发明的主题。
包含核苷酸序列SEQ ID No.1、SEQ ID No.2、SEQ ID No.3、SEQ IDNo.4、SEQ ID No.5、SEQ ID No.6、SEQ ID No.7、SEQ ID No.8中的至少一个的质粒、克隆和/或表达载体，以及包含这些核苷酸序列中至少一个的核酸也是本发明的另一主题。
本发明也提供由上述质粒或载体转化的宿主细菌。
本发明还涉及构建上述菌株的方法，其特征在于这些菌株是通过使用包含核苷酸序列SEQ ID No.1、SEQ ID No.2、SEQ ID No.3、SEQ ID No.4、SEQ ID No.5、SEQ ID No.6、SEQ ID No.7、SEQ ID No.8中至少一个的质粒或载体，或包含这些核苷酸序列中至少一个的核酸的载体转化获得的。
本发明还提出了包含上述至少一个菌株或包含根据本发明方法获得的至少一个菌株的细菌组合物。
最后，本发明涉及包含根据本发明的至少一个菌株或根据本发明方法获得的至少一个菌株或细菌组合物的食品或药物组合物。
本发明在将介质组织形成方面具有很多优点。尤其是，它使从例如发酵乳获得凝胶成为可能，该凝胶浓稠、粘稠、有涂布性、有粘性和耐搅拌并且不是粒状的。
阅读下列说明书和纯粹为例证而给出的非限制性实施例后，本发明的其它优点和特征将变得显而易见。
本发明首先涉及包含选自由核苷酸序列SEQ ID No.1、SEQ ID No.2、SEQ ID No.3、SEQ ID No.4、SEQ ID No.5、SEQ ID No.6、SEQ ID No.7、SEQ ID No.8组成的组的至少一个序列的乳酸细菌菌株。
该乳酸细菌是革兰氏阳性原核生物，属于厚壁菌门分类群。它们是异养的和亲化学器官的；一般是厌氧的或耐氧的，它们的新陈代谢可以是同型或异型发酵；该乳酸细菌基本上通过glucidic底物发酵产生乳酸。缺少过氧化氢酶，该乳酸细菌组成气球菌属(Aerococcus)、肠球菌(Enterococcus)、乳球菌属(Lactococcus)、明串珠菌属(Leuconostoc)、酒球菌属(Oenococcus)、小球菌属(Pediococcus)、链球菌属(Streptococcus)、四联球菌属(Tetragenococcus)、漫游球菌属(Vagococcus)和魏斯氏菌属(Weissella)的球菌形式，或乳杆菌属(Lactobacillus)和肉杆菌属(Carnobacterium)的棒状形式的细菌异种群。该名称乳酸细菌常常延至其它相关细菌，如双歧杆菌属(Bifidobacterium)。
在适于本发明的乳酸细菌菌株当中，可以提及链球菌属(Streptococcus)、乳球菌属(Lactococcus)、乳杆菌属(Lactobacillus)、明串珠菌属(Leuconostoc)、小球菌属(Pediococcus)和双歧杆菌属(Bifidobacterium)。
根据本发明优选的乳酸细菌菌株是嗜热链球菌。
嗜热链球菌是天然存在于牛奶中的种并广泛用于食品中，尤其是乳业，因为它使牛奶变酸和达到一定稠度成为可能。它是同型发酵嗜热菌。
然后，本发明涉及2003年2月26日保藏在国立微生物保藏中心，保藏号为I-2980的嗜热链球菌菌株。
核苷酸序列SEQ ID No.1、SEQ ID No.2、SEQ ID No.3、SEQ ID No.4、SEQ ID No.5、SEQ ID No.6、SEQ ID No.7、SEQ ID No.8也是本发明的主题。
核苷酸序列SEQ ID No.2至SEQ ID No.8形成参与多糖(PS)合成的大约14,350个碱基对的操纵子的一部分。这个操纵子包括在序列SEQ IDNo.1中。这意思是根据本发明，序列SEQ ID No.2至SEQ ID No.8包括在序列SEQ ID No.1中。
已经确定了根据本发明的操纵子结构(参见图1)。在deoD(操纵子PS的上游)和orf14.9(下游)基因之间，已经鉴定了称作eps13A、eps13B、eps13C、eps13D、eps13E、eps13F、eps13G、eps13H、eps13I、eps13J、eps13K、eps13L、eps13M、eps13N、eps130、eps13P和IS1193的17个ORFs(开放阅读框)。位于“正义”链上的前16个ORFs可能编码参与生产胞外或荚膜多糖的多肽；位于“反义”链上的第17个ORF可能编码属于IS1193家族的功能转座酶(插入序列)。
有可能对每一ORF进行命名和定位它。下面给出每一ORF的名称，然后其次是推定的衍生蛋白的功能，和最后，第三是包含这个ORF的区域的位置(与如序列表表示的序列SEQ ID No.1有关)：
-eps13A：转录调节子(342..1802)
-eps13B：多糖的聚合(链长调节)和/或输出(1803..2534)
-eps13C：多糖的聚合(链长调节)和/或输出(2543..3235)
-eps13D：多糖的聚合(链长调节)和/或输出(3245..3985)
-eps13E：Undecaprenyl-磷酸盐糖基转移酶(4042..5409)
-eps13F：Undecaprenyl-磷酸盐糖基转移酶(5611..6195)
-eps13G：Undecaprenyl-磷酸盐糖基转移酶(6251..6634)
-eps13H：β-1，4-半乳糖基转移酶(6643..7092)
-eps13I：β-1，4-半乳糖基转移酶(7092..7607)
-eps13J：鼠李糖基转移酶(7597..8493)
-eps13K：糖基转移酶(87639797)
-eps13L：重复单位聚合酶(9827..10969)
-eps13M：重复单位聚合酶(10984..11793)
-eps13N：糖基转移酶(11844..12578)
-eps13O：糖基转移酶(12633..13016)
-eps13P：跨膜转运蛋白(13049..14482)
-IS1193：转座酶(互补物(14614..15870))
本发明还涉及包含选自由核苷酸序列SEQ ID No.1、SEQ ID No.2、SEQ ID No.3、SEQ ID No.4、SEQ ID No.5、SEQ ID No.6、SEQ ID No.7、SEQ ID No.8组成的组的至少一个序列的核酸。在本发明的意思中，包含上面所列序列中至少一个的核酸的意思是包含至少一个ORF的核酸，该ORF的翻译产物与序列SEQ ID No.1至SEQ ID No.8中鉴定的ORFs所得到的至少一个多肽序列具有显著的序列相似性(残基位置之间最大一致序列的比对后，相同残基大于或等于80％的百分比)。
核苷酸序列SEQ ID No.1、SEQ ID No.2、SEQ ID No.3、SEQ ID No.4、SEQ ID No.5、SEQ ID No.6、SEQ ID No.7、SEQ ID No.8可以通过基因工程插入载体，尤其是通过本领域技术人员普遍认识的重组DNA技术。
本发明还涉及包含选自核苷酸序列SEQ ID No.1、SEQ ID No.2、SEQID No.3、SEQ ID No.4、SEQ ID No.5、SEQ ID No.6、SEQ ID No.7、SEQID No.8组成的组的至少一个序列或如上定义的核酸的克隆和/或表达载体。
根据本发明的优选载体是质粒。它可以是复制型或整合型质粒。
从这些载体和/或质粒开始，为了将这些载体和/或质粒包括入细菌中，有可能转化细菌。可以通过电穿孔法或通过结合技术以对本领域技术人员来说的标准方式进行这种转化。
本发明也涉及由上面定义的质粒或载体转化的宿主细菌。
优选，转化的细菌是乳酸细菌。尤其是，它们是选自链球菌属(Streptococcus)、乳球菌属(Lactococcus)、乳杆菌属(Lactobacillus)、明串珠菌属(Leuconostoc)、小球菌属(Pediococcus)和双歧杆菌属(Bifidobacterium)的乳酸细菌。
根据本发明优选的乳酸细菌菌株是嗜热链球菌。
本发明还涉及构建根据本发明的菌株或转化的宿主细菌的方法，其特征在于它们是通过使用包含选自核苷酸序列SEQ ID No.1、SEQ ID No.2、SEQ ID No.3、SEQ ID No.4、SEQ ID No.5、SEQ ID No.6、SEQ ID No.7、SEQ ID No.8组成的组的至少一个序列的载体，或包含选自核苷酸序列SEQ ID No.1、SEQ ID No.2、SEQ ID No.3、SEQ ID No.4、SEQ ID No.5、SEQ ID No.6、SEQ ID No.7、SEQ ID No.8组成的组的至少一个序列的核酸的载体转化获得的。
根据本发明的方法，优选载体是质粒。
有利地，根据本发明的方法，转化之后是通过至少一个重组结果插入到菌株或宿主细菌的基因组。
本发明还涉及包含根据本发明的至少一个菌株或包含根据本发明方法获得的至少一个菌株或包含根据本发明转化的至少一个细菌的细菌组合物。
细菌组合物的意思是不同菌株的混合物，尤其是发酵物，或发酵剂。
根据本发明优选菌株的混合物是嗜热链球菌与其它嗜热链球菌的混合物，或嗜热链球菌与德氏乳杆菌保加利亚亚种的混合物，或嗜热链球菌与其它乳杆菌和/或与双歧杆菌的混合物，或嗜热链球菌与乳球菌的混合物，或嗜热链球菌与乳酸细菌的其它菌株和/或酵母的混合物。
本发明还涉及根据本发明的菌株或根据本发明方法获得的菌株或根据本发明的细菌组合物为生产食品或食品成分的用途。
作为根据本发明的优选食品或食品成分，可以提及乳制品、肉制品、谷类制品、饮料、起泡剂或粉剂。
本发明还涉及包含根据本发明的至少一个菌株或根据本发明方法获得的至少一个菌株或根据本发明的细菌组合物的食品或药物组合物。
包含根据本发明的至少一个菌株或根据本发明方法获得的至少一个菌株或根据本发明的细菌组合物的乳制品也是本发明的主题。
在生产乳制品的情况下，后者以这个领域通常的方式进行，和尤其是通过掺入根据本发明的菌株进行乳制品的发酵。
作为根据本发明的乳制品可以提及发酵乳、酸奶、成熟奶油、乳酪、清爽干酪、乳制饮料、乳制品截留物、融化干酪、奶油甜点、酪农干酪或婴儿奶。
优选根据本发明的乳制品包含动物和/或植物来源的奶。
作为动物来源的奶，可以提及母牛、母羊、山羊或水牛的奶。
作为植物来源的奶，可以提及根据本发明可以使用的植物来源的任何可发酵物质，尤其是来源于大豆种子、大米或麦芽。
附图说明
图1表示与其它已经知道的嗜热链球菌菌株的PS操纵子相比，根据本发明菌株的PS操纵子的基因结构。
图2表示由本研究的不同菌株得到的发酵乳的感觉数据获得的主要成分分析(PCA)的析因设计1-2。
图3表示对于本研究的每一菌株匙上质地的描述信息获得得分的平均值。Newman-Keuls检验结果的解释：相同字母相关的菌株之间的差异不显著。
图4表示对于本研究的每一菌株口中质地的描述信息获得得分的平均值。Newman-Keuls检验结果的解释：相同字母相关的菌株之间的差异不显著。
具体实施方式
现在将描述具体但是非限制性的实施例。
实施例
1/嗜热链球菌CNCM I-2980的PS操纵子的序列
携带嗜热链球菌菌株CNCM I-2980的PS操纵子的DNA片段通过PCR(聚合酶链式反应)扩增所述菌株提取的基因组DNA获得。用Mastercycler热循环仪(Eppendorf)，使用LA-Tag DNA聚合酶(BioWhittaker/Cambrex)和下列引物：5’-GGGTGAACGTATCTCAGTAATGGGGACTGG-3’和5’-CCTGAGTTATGCGACGATTACTTGGCTG-3’进行扩增。这个扩增的实验条件是下列：14个循环交替的98℃变性30秒和68℃杂交延伸15分钟，然后16个循环交替的98℃变性30秒和68℃杂交延伸15分钟，每个循环增加15秒，然后72℃另外延伸循环10分钟。根据片段克隆方法对PCR产物测序。
2/与已经知道的嗜热链球菌菌株相比，根据本发明菌株的表征
·PS操纵子的序列
嗜热链球菌菌株CNCM I-2980的PS操纵子的序列是在该菌株的纯化基因组DNA模板存在下，使用文献中描述的通常构架嗜热链球菌中PS操纵子的保守基因(编码嘌呤核苷酸磷酸化酶的deoD和功能未知的orf14.9)的两个特异性引物，通过PCR合成大约17,100个碱基对的DNA片段获得的。用序列表给出了包含基因deoD和orf14.9之间相当于序列SEQ IDNo.1的序列。
·PS操纵子的基因结构
图1图解显示了通过菌株CNCM I-2980的PS操纵子的核苷酸序列分析确定的它的基因结构，以及嗜热链球菌其他7个菌株的PS操纵子结构。对于不同的结构，用菌株名称和GENBANK登录号(在括号中)鉴别，箭头表示位置，基因的大小和方向是从起始密码子至终止密码子。图1的图例说明给出了箭头内颜色和/或部件的意义。
·文献比较
菌株CNCM I-2980的PS操纵子的结构分析表明它具有类似于已经知道的PS操纵子的总体结构(参见图1)；可能参与PS操纵子转录调节的第一个ORF，后面是可能参与PS重复单位聚合和/或它们的输出调节的3个ORFs，后面是编码糖基转移酶和确保重复单位装配的聚合酶的11个ORFs，它们自己后面是可能参与重复单位通过质粒膜运输的1个ORF。
菌株CNCM I-2980的PS操纵子的基因环境也类似于其它已知的PS操纵子的基因环境：上游的ORF deoD编码嘌呤核苷酸磷酸化酶，和下游的相反方向上ORFs IS1193和orf14.9分别编码转座酶(属于IS1193家族插入序列，可动基因元件)和功能未知的蛋白。
然而，菌株CNCM I-2980的操纵子的ORFs可能编码的蛋白和公共数据库(GENBANK)中可获得的蛋白之间进行的序列比较表明菌株CNCMI-2980的PS操纵子的基因内容在其远端部分是新的。
-嗜热链球菌，和目前已知的链球菌属更常见菌株的PS操纵子的近端部分，包含称作epsA(或cpsA，或capA，或wzg)、epsB(或cpsB，或capB，或wzh)、epsC(或cpsC，或cpaC，或wzd)和epsD(或cpsD，或capD，或wze)的4个ORFs。对于这4个ORFs的每一个，菌株之间其得到的多肽产物具有显著的序列相似性。在这个水平，由菌株I-2980的PS操纵子的ORFs eps13A、eps13B、eps13C和eps13D得到的多肽产物不能与其它PS操纵子得到的产物有显著不同(相同氨基酸的百分比大约或等于94％)。
-就在ORF epsD之后，在嗜热链球菌的大多数已知PS操纵子中存在ORF epsE(或cpsE，或capE，或wchA)。在这种情况下，同样，来源于不同已知序列的同种多肽产物之间的序列相似性是显著的；菌株CNCMI-2980的ORF eps13E的翻译产物与来源于其它菌株的它的同系物强烈类似。
尽管已经描述过，但是在嗜热链球菌的PS操纵子的不同已知结构当中，菌株CNCM I-2980的PS操纵子的下列4个ORFs(eps13F、eps13G、eps13H和eps13I)的结构较少见。有时不完全地在菌株IP6757(GENBANK登录号AJ289861)、“VIl型”(GENBANK登录号AF454498)和“III型”(GENBANK登录号AY057915)中发现这个结构。
-在该操纵子的远端部分，7个ORFs eps13J、eps13K、eps13L、eps13M、eps13N、eps13O和eps13P对菌株CNCM I-2980的PS操纵子是新的和特异性的。尽管细微，但是在蛋白水平它们的序列相似性，或在特异蛋白单位存在的某些情况下，使得赋予这些ORFs的产物的推定功能成为可能：ORFs eps13J至eps130的产物具有潜在的糖基转移酶或聚合酶活性，ORFeps13P的产物具有PS的跨膜转运活性。
3/嗜热链球菌菌株CNCM I-2980的对比流变学试验
使用的嗜热链球菌菌株CNCM I-2423、CNCM I-2429、CNCM I-2432、CNCM I-2978和CNCM I-2979是来自Rhodia Food保藏的菌株。它们用于大部分发酵乳或酸奶的工业生产并且公认它们的组织形成性能。它们是文献中描述菌株的代表。此后它们同菌株CNCM I-2980比较起来研究并认为是当前用于农食品工业的组织形成菌株代表。
嗜热链球菌菌株RD736和RD676是Rhodia Food工业菌株，具有低组织形成能力的声誉。它们可以生产的多糖和它们的PS操纵子未知。此后它们同菌株CNCM I-2980比较起来研究并认为是非组织形成菌株的代表。
使用的发酵乳是通过给100ml UHT脱脂乳(Le Petit
)补充3％(体重/体积)的脱脂奶粉获得的。在90℃巴氏灭菌10分钟达到溶液的灭菌，在奶的中心测量该温度。以10E+6cfu/ml(菌落形成单位/ml)的比率给由此获得的发酵载体接种待检测的菌株，然后在43℃(在水浴中)培养直至pH达到4.6。连续记录pH的监测。由此获得的发酵乳置于6℃通风烘箱中，直至分析。
进行两种类型的流变性测量：粘度和流量。在6℃贮存1、7、14和28天后，在8℃对发酵乳进行粘度测量。使用的仪器是固定在Helipath支架(Brookfield Engineering Laboratories，Inc.)上的RVF-type
粘度计(Brookfield Engineering Laboratories，Inc.)。该粘度计安装有C型针和施加于该针的振动速度是10转/分。在6℃贮存14天并且预先已经搅拌过后，在8℃对发酵乳进行流量测量。使用的仪器是装备着同轴圆筒(半径1＝15mm、半径2＝13.83mm、高度＝32mm、气隙＝2mm)的AR1000-N血流速度计(TA Instruments)。对于上升段，连续扫描中应用的应力从0至60Pa变化，根据线性模型，持续时间1分钟。对于下降段，连续扫描中应用的应力从60至0Pa变化，根据线性模型，持续时间1分钟。考虑的值是触变域和屈服点；后者根据卡森模式计算。
在6℃贮存1、7、14和28天后测量由嗜热链球菌菌株CNCM I-2980得到的发酵乳的粘度(表1)。贮存一天后测量的粘度值是53.3Pa-s。这个值随表明由嗜热链球菌菌株CNCM I-2980得到的发酵乳的稳定性的时间变化很小。相比之下(表1)，测量用具有弱组织形成能力声誉的菌株RD736和RD676生产的发酵乳的粘度包括在26和30Pa-s之间。试验的其它菌株产生比由菌株CNCM I-2980得到的粘度较低的发酵乳。有可能区分产生40Pa.s级别(CNCM I-2979、CNCM I-2423和CNCM I-2432)粘度的一组菌株，和产生50Pa.s级别粘度的第二组-CNCM I-2980所属的组。
表1：由试验的不同菌株得到的发酵乳在6℃不同贮存时间后的粘度和pH
流量测量使定义对于发酵乳流变性描述的两个重要流变性描述信息(屈服点和触变域)成为可能(表2)。对于由菌株CNCMI-2980得到的发酵乳，屈服点和触变域的平均值分别是5.89Pa和488Pa/s。这些值显著不同于由认为是非组织形成菌株得到的发酵乳获得的值。(RD676和RD736)例如对于由菌株RD676得到的发酵乳，这些平均值分别是17.01Pa和17083Pa/s。在认为是组织形成菌株的情况下，屈服点和触变域值非常接近于由菌株CNCM I-2980得到的值，但是然而明显更大，表明比菌株CNCMI-2980更大的组织形成能力。
表2：由试验的不同菌株得到的发酵乳在6℃贮存14天后，使用AR1000-N装置测量的屈服点和触变域值(3次重复的平均值)
| 菌株 | 屈服点(Pa) | 触变域(Pa/s) |
| CNCM I-2980 | 5.89 | 488 |
| CNCM I-2429 | 13.32 | 1215(2个值) |
| CNCM I-2978 | 10.51 | 728 |
| CNCM I-2432 | 12.27 | 1245 |
| CNCM I-2423 | 8.86 | 1344 |
| CNCM I-2979 | 13.56 | 1786 |
| RD676 | 17.01 | 17083 |
| RD736 | 15.91 | 33100 |
4/与参照菌株比较，嗜热链球菌菌株CNCM I-2980的感觉特征
在6℃贮存14天后通过感觉分析评估发酵乳。维持在12℃的最佳味道温度，9位专家的陪审团以0至6点的非构造的线性刻度进行发酵乳的定量描述性分析。以几天的间隔重复这个感觉课题分析。所选择的和预先训练的鉴定人以匙上质地的4个描述信息：脆性、耐搅拌性、粘性、粒度，和以口中质地的4个描述信息：熔化性、粘稠性、浓稠度、涂布性进行它们的评估。通过固定模式-双因素方差分析(称作ANOVA)，随后是对每一描述信息的5％α阈的Newman-Keuls均数比较检验法评估感觉差异。为了直观化所产生的空间，进行各个菌株的感觉描述信息作为变量的主要成分分析(PCA)。上升等级分类(AHC)使根据PCA分离菌株组成为可能。这些统计分析使用的软件是
(Biosystèmes)、
和Uniwin
由嗜热链球菌菌株CNCM I-2980得到的发酵乳数据与进行由其它参照菌株得到的发酵乳的数据比较。表3示出了由采用的不同菌株质地描述信息得到的平均值和表4和图3和4列出了由ANOVA和平均比较检验法出现的显著性差异
表3：通过陪审团对于由本研究的不同菌株得到的发酵乳的质地描述信息的感觉分析分配的分数的平均值
表4：Newman-Keuls检验以5％进行的匙上质地和口中质地的每一描述信息的平均值比较。结果解释：相同字母相关的菌株之间的差异不显著。
图3和4显示了表4中获得结果的直方图表示。
对于所有描述信息，认为是非组织形成的菌株RD676和RD736与其它菌株有显著区别。在组织形成菌株当中，菌株CNCM I-2980除了粒度的所有描述信息具有清晰和显著区别，粒度的情况不能与菌株I-2978和CNCM I-2423区别。
PCA使菌株位置作为它们的涉及感觉描述信息的距离的函数成为可能。
图2中PCA的设计1-2表示97.3％的产生空间。成分1与两组感觉变量相对照。变量：耐搅拌性、在口中的浓稠度、在口中的涂布性、在匙上的粘性和在口中的粘稠性组成的第一组说明右边的成分1。由变量：在口中熔化性、在匙上脆性和在匙上成粒度组成的第二组说明左边的成分1。第一组变量与第二组反向相关。这些变量使分析菌株在这个平面图上的位置成为可能。此外，AHC分析使对析因设计1-2上以点圆形式表示的不同组的菌株进行分类成为可能。
析因设计对照了提供不同质地性能的几组菌株。从这些分析显露出菌株RD736和RD676给予发酵乳匙上脆性和粒状质地和口中熔化质地。同样地，与另一组菌株比较，它们在口中不产生浓稠、粘稠或涂布质地，在匙上既无抗性也无粘性。因此它们产生非组织形成发酵乳。相反，菌株CNCM I-2429、CNCM I-2432和CNCM I-2979产生平均稠度的发酵乳；菌株CNCM I-2423和CNCM I-2978产生稠度的发酵乳，和菌株CNCM I-2980产生高稠度的发酵乳。这些分析表明与所有参照菌株相比，菌株CNCMI-2980在发酵乳中产生非常特殊的质地特征。
5/嗜热链球菌菌株CNCM I-2980对噬菌体的抗性的对比试验
溶解血小板法确定菌株对噬菌体的敏感性。用100μl待试验菌株的培养物和100μl含有待研究噬菌体的适当稀释度的血清接种在补充10mMCaCl2的5ml M17葡糖琼脂培养基中不至于溢出(琼脂0.6％重量/体积)。全部倒在补充10mM CaCl2的固化M17葡糖琼脂培养基(琼脂1.5％重量/体积)表面上。在42℃培养16小时后，在溶解血小板存在下评估菌株对噬菌体的敏感性。溶解血小板的缺乏象征这个菌株对试验的噬菌体有抗性。菌株对噬菌体(也称作噬菌体型)的敏感性谱由所有对所研究噬菌体的敏感性和抗性组成。
下表5定义了本研究使用的噬菌体和它们的菌株的来源/繁殖。这些是来源于Rhodia Food保藏的菌株和噬菌体。根据对参照组织形成菌株vis-à-vis的传染性选择噬菌体。
表5：噬菌体
| 噬菌体名称 | 菌株来源 |
| 2972 | CNCM I-2423 |
| 4082 | RD729 |
| 4074 | CNCM I-2429 |
| 4154 | CNCM I-2429 |
| 1272 | CNCM I-2978 |
| 4128 | RD852 |
| 1255 | CNCM I-2432 |
| 1765 | RD728 |
| 4121 | RD862 |
为了评估菌株CNCM I-2980关于与噬菌体相关问题的工业益处，评估菌株CNCM I-2980的噬菌体型并与参照组织形成菌株的噬菌体型相比。表6显示了用溶解血小板法确定的菌株对这些不同噬菌体(噬菌体型)的敏感性。看来本研究的六个菌株具有不同的噬菌体型。尤其是，菌株CNCMI-2980具有与试验的其它组织形成菌株不同的噬菌体型。事实上，菌株CNCM I-2980不能被试验的噬菌体感染。
表6：试验的菌株的噬菌体型
+：对试验的噬菌体敏感；-：对试验的噬菌体有抗性
序列表
<110>罗迪亚化学
<120>组织形成乳酸细菌
<130>0910613
<150>FR 0303242
<151>2003-03-17
<160>8
<170>PatentIn version 3.2
<210>1
<211>16032
<212>DNA
<213>Streptococcus thermophilus
<400>1
tttgtaaaag gacgccattt ggtcgtcctt ttgtgttgta gctaatatct gttcgaagtg 60
ataataagtt aaaatttttc aaactactag aaaaaataaa aatatttgga agaagaagac 120
ttttaataaa taggtaaata tctgacaatt taaagtttaa ctactaaaaa tgtgaaagat 180
agttcacaat ataatggaaa atgatataaa ttaaatgatt gatatcataa tgaaaacgtt 240
tttttgtttt tttttgaaaa aaatgacaat tgaaatgaaa ttgtattaat gttacaataa 300
taatggggaa tacttaattt taatttttag gagcaattta tatgagttcg cgtacgaatc 360
gtaagcaaaa acgtacgggt aatagatcat gggggatggt caacgttgga ttgaccattc 420
tgtatgctat tttagcattg gtcttattat tcaccatgtt caattataat ttcctatcct 480
ttaggttttt gaacatcatt atcactattg gtttgttggt agttcttgct attagcatct 540
tccttcagaa gactaagaaa tcaccactag tgacaacggt tgtattggtt atcttctcgc 600
tagtttctct ggttggtatt tttggtttta aacaaatgat tgacatcact aaccgtatga 660
atcagacggc agcattttct gaagtagaaa tgagcatcgt ggttcctaag gaaagtgaca 720
tcaaagatgt gagccagctt actagcgtac aggcacctac taaggttgat aagaacaata 780
tcgagatctt gatgtcagct ctcaaaaaag ataaaaaagt tgatgttaaa gttgatgatg 840
ttgcctcata tcaagaagct tatgataatc ttaagtctgg caaatctaaa gctatggtct 900
tgagtgggtc ttatgctagc ctactagagt ctgtcgatag taattatgct tcaaatctaa 960
aaacaattta tacttataaa attaaaaaga agaatagcaa ctctgcaaac caagtagatt 1020
caaaagtctt caatatttat attagtggta ttgataccta cggttcgatt tcaacagtgt 1080
cacgttcaga tgtcaatatt attatgacag taaacatgaa tacacataag attctcttga 1140
cgactactcc acgtgatgca tacgttaaga ttcctggtgg tggagcagac cagtatgata 1200
aattaaccca cgcaggtatt tatggcgttg aaacatctga acaaactctg gaagatctat 1260
atggtactaa gattgattac tatgcccgaa ttaacttcac atctttcctt aagttgattg 1320
accaacttgg tggtgtgaca gtccataatg atcaagcttt cacaagtctt catgggaagt 1380
ttgatttccc agttggagat atccaaatga attcagagca agcacttgga tttgttcgtg 1440
aacgctatag tttagatggc ggagataatg atcgtggtaa aaaccaggaa aaagtcattt 1500
ctgcgattgt aaacaagttg gcttctctaa agtctgtatc aaactttact tcaatcgtta 1560
ataatctcca agactctgtt cagacaaata tttctttgga taccattaat gctttggcta 1620
atacacaact tgattcaggt tctaaattta cggtgacttc tcaagcagta actggtacag 1680
gttcaaccgg acaattgacc tcttatgcga tgccaaattc tagtctttac atgatgaaac 1740
tagataattc gagtgtggca agtgcctctc aagctatcaa aaatctaatg gaggaaaaat 1800
aagtgattga cgttcactca catattgttt ttgatgttga tgatggtcct aaaactttag 1860
aagaaagttt agatctcatt ggtgaaagtt acgcccaggg ggtacgtaag attgtttcaa 1920
catcccatcg tcgtaagggg atgtttgaga ctccagagga taaaattttt gccaactttt 1980
ctaaggtaaa agcagaagca gaagcacttt atccagactt aactatttat tatggaggtg 2040
aactttatta caccttagac attgtggaga aacttgaaaa gaatctcatt ccgcgcatgc 2100
acaacactca atttgctttg attgagttta gtgctcgcac atcttggaaa gaaattcata 2160
gtgggcttag taatgttttg agagcggggg taacgcctat tgttgctcat attgagcgct 2220
atgatgccct cgaagaaaat gctgaccgtg ttcgagaaat catcaacatg gggtgctata 2280
ctcaagttaa tagctctcat gttctaaaac caaagctctt tggagataaa gataaagtaa 2340
gaaaaaaacg tgttcgttat tttttggaga aaaatttggt tcatatggtt gctagcgaca 2400
tgcataatct tgggccgaga ccaccattta tgaaagatgc ttatgaaatt gttaaaaaga 2460
actacggccc caaacgtgct aagaatcttt ttattgaaaa tcccaaaaca ttactagaaa 2520
atcaatattt ataggagata ttatgaatca agataacact aaaagtgatg aaatcgacgt 2580
actagcattg ctacataaac tttggacgaa gaagcttttg attcttttca cagcttttta 2640
tttcgctgct ttcagtttct taggtactta tttctttatc caaccaacat atacatcaac 2700
aacgcgtatc tatgttgtta atcaggcaac agataataat aatctttctg ctcaagattt 2760
gcaagctggt acctatttgg caaatgacta taaagagttt attacatcaa atgatgtatt 2820
atcagaagtt attaaagatg aaaaattgaa tttgagtgag gcagaactgt ctaaaatggt 2880
ttcagttaat attcctactg atactcgtct tatttcaatt tctgttaatg ctaaaactgg 2940
tcaagatgcg caaacacttg ctaataaggt tcgtgaagtt gcttcaaaaa aaatcaagaa 3000
ggtgacaaaa gttgaagatg tcacaacgct cgaagaagct aaattgccag agtcaccatc 3060
ttcaccaaat atcaaacgta atgtgcttct tggggcagtg cttggaggat tccttgcagt 3120
ggttggtgta ttggtacgtg aaatcctaga tgatcgtgtt cgccgtccag aagatgtgga 3180
agatgccctt ggaatgacac ttcttggaat tgtccctgat acagataaga tttaaggaga 3240
agaaatgcct ttattaaagt tagttaaatc aaaagtagac tttgctaaaa agacggaaga 3300
gtattataac gctattcgca caaatattca attttctggt gctcagatta aagtgattgc 3360
gattagctct gttgaaactg gtgaaggaaa atcaacgaca tctgttaact tggcgatttc 3420
atttgctagt gttgggctcc gaacacttct gattgatgcg gatacgcgta attctgtttt 3480
gtcgggtaca tttaaatcaa atgagcctta taaaggtctt tcaaatttcc tttcaggaaa 3540
tgccgatcta aatgaaacga tttgccaaac tgatatttct ggtttagatg ttattgcatc 3600
tggtcctgta ccacctaatc caacaagtct tttgcaaaat gacaatttta gacatttgat 3660
ggaagttgct cgtagtcgtt atgattatgt tatcatttat acaccaccaa ttggtctggt 3720
cattgatgct ggtattattg cccatcaggc tgatgctagt cttttggtta cagcagctgg 3780
aaaaattaaa cgtcgtttcg taactaaggc ggtcgaacaa ttgaaacaaa gtggttctca 3840
gttcttaggt gtcgtcctta ataaagttga catgacagtt gataaatatg gatcatatgg 3900
ttcttacgga tcatatggtg agtacgggaa aaaaacagac caaaaagaag gtcattcaag 3960
agcacatcgt cgtagaaaag gatagcatta atggggatga tgcggctcct tataccttaa 4020
cagattaaaa aggggtttag agtgaaagaa aaacaagaaa ttcgtcgcat tgaaattggt 4080
attatacagt tggttgtggt tgttttcgca gccatggtag ctagtaaaat accatataca 4140
gagattaccc aaggaagtat tgtcctttta ggtgtcgtac atgtagtgtc ttactatatc 4200
agtagttatt atgaaaatct taagtataga ggctacttgg atgaactcat tgcaactgtc 4260
aaatattgtt tcatatttgc tctaattgca acatttctct cgttttttgc agatggaagt 4320
ttttcaatct cacgtcgcgg acttctttac gtcaccatga tttcaggtgt tctcttatac 4380
gttacaaata ctgttcttaa gtatttccgc tcatctattt atacacgtcg taaaagtaac 4440
aagaatattc tcttgatttc tgatcaggca cgtcttgata atgttttatc tcgtatgaag 4500
gacaatatgg atggtaggat ttcagcagtt tgtgtcttgg ataatcctta tttcactgat 4560
ccatttatca agagtgttaa acctgaaaat ttgattgaat atgcgacaca ctcagtagta 4620
gaccaagttt tgattaatct gccaagtgag cagtacaaga tttgggatta tgcgtcacca 4680
tttgaactta tgggaatccc agtatccatt aatttgaatg cccttgaatt tatgagtcag 4740
ggtgaaaaac gtatccaaca attgggtcct ttcaaagttg ttacgttttc tacgcatttt 4800
tatagctatg gagatatctt ggcgaaacgc ttcctcgata tctgtggagc tctagttggt 4860
ttggtgctct gtgggattgt tggaatcttc ctttatccac ttattcgtaa ggatggtggg 4920
ccagccattt ttgctcaaga ccgtgtggga gaaaatggac gtatcttcaa gttttataaa 4980
ttccgttcta tgcgtgttga tgctgaggaa attaaaaagc aattgatgga taaaaatcaa 5040
atgtctggtg gtatgtttaa gatagacaat gatccacgta ttaccaaaat tggacatttc 5100
attcgtaaaa caagtcttga tgaacttcca caattttgga atgttctaaa aggtgatatg 5160
agcttggtag gaacacgtcc accaacattg gatgagtacg aatcttatac accggaacaa 5220
aaacgtcgtt taagttttaa acctggtatc acaggtcttt ggcaagtaag tggacgaagt 5280
gaaattactg attttgatga agttgtaaaa ctagatgttg cttatatgga tgggtggaca 5340
atctggcgag atatcaaaat cttattgaaa acgattaaag tagtagtaat gaaggatgga 5400
gcaaaatgat aacttcaaag atgattagat gagaaaaata atttatattg ttgcttctaa 5460
agggattcct gcaaaatatg gtggatttga gacctttgtt gagaagttga cagagttcca 5520
ataagacaaa gatatccaat attatgtagc ttgtatgcgt gaaaactctg caaaatcagg 5580
aattagagca gatactttat caactgcact gtgcgtagat acaaatatct attccttatg 5640
actgctgaac aacagaagaa tacattagtc attaccacca ctgagagatt gcgattaatg 5700
gaggggctct ttgagtcaga tcaacttcct atcaaatatt tggcaggtat agtagtcatc 5760
ggagatggtg aagtggcgtt tccagaggga gtcccaataa ttccttttga tgatgcgatt 5820
gactttgcga ctcatgaagt tgtcgaccat gtgtttatca acttaccgag tgaacattac 5880
gatctcaaac atcttgtttc cgattttgaa gtcatgggta ttgatgtgag tgtagatatt 5940
aacttatttg atttcagggc tttaaaaaat aaaaaaatca aacaagttgg agaccatagt 6000
atcgtgactt ttaactccaa ttactacaaa catagccata tctttctaaa gcgcatgttg 6060
gatatctttg gggctgtgat tggtcttcta atttgtggtc tggtcgggat tgtcttagct 6120
cctatcattc ggaaagatgg aggaacctgc tattttcgtt cagaaacgag tagggaaaaa 6180
cggtcgtatc tttaattttt ataaattccg ttctatgtat atagatgcag aaaaacggaa 6240
aaaagaactt atggctcaaa accagatgca gggtggtatg tttaaaatgg ataatgaccc 6300
acggattaca ccaattggtc agtttatccg aaaaacaagt ctggatgaat tgccacagtt 6360
ttataatgta ctagttggtg atatgagctt ggtaggtact cgtccaccga cagtggatga 6420
atttgaaaag tatacaccaa gtcaaaaacg acgacttaat tttaaaccgg ggattaccgg 6480
cttatggcaa gttagtgggc gtagcaatat tacggacttt gacgaagtag ttaaactcga 6540
tgttgaatac atagataact ggtcaatttg gtcggatatc aagatattgt tgaagactat 6600
cttcgtagta tttaaaaaag agggaagtaa gtagagtata tcatgaaagt ttgtttagta 6660
ggttcttctg gtgggcattt ggcacacttg aatatgctta aacctttttg gagtgaacag 6720
gaccgctttt gggttacctt tgataaagaa gatgcaagaa gtattttaaa agatgagcag 6780
ttttatccgt gctattttcc tactaacaga aattttaaaa atttagtaaa gaatactttt 6840
ttagcactta aaattttaag aaaagaaaga cctgacgtta ttatttcatc aggagcagcg 6900
gtagcagttc cgttttttta tcttggtaaa ctgtttggag cgaaaacggt ttatatagaa 6960
gtatttgata gaatagataa accgacagta actgggaaat tggtttatcc agtgacagat 7020
aaatttattg ttcagtggga ggagatgaaa actgtctatc ccaaagctat taatctgggg 7080
agtatttttt aatgattttt gttacagtgg gaacccacga acagcccttt aataggctta 7140
ttaaggaagt tgatcgttta aaaaaagaag gtattattac agatgaggtt tttattcaga 7200
caggtttttc aacttatgaa cctcaatact gtgactggaa aaaacttatc tcttattctg 7260
agatggacaa ctacatgact cactctgata tcattataac ccatggtggt ccagcgacat 7320
tcatgggagc aattgccaaa ggcaaaaaac caattgttgt tccaagacaa gaaaaatatg 7380
gagaacatgt aaatgatcat cagttagagt ttgcagaaca ggtttctgga cgttttggga 7440
gtattattgt tgtagaagat attacttcac tctgtgaaat tttattaaaa ggaagtaacg 7500
ttatttgcca aaagcagagt caattttacc ctaaaggatc acagtttatt tttaaattca 7560
aagagatagt tgatgaactt gtagaaggag agattcgtga aacctaaaat acttgtactt 7620
atggcaacct ataatggagg aaaatactta cgagagcaat tagatagtgt ttttttacaa 7680
aaaaatgtgg atattactgt cctggttcgt gacgatggtt caattgacaa tacgtgtgct 7740
attcttgatg aatatagtaa gagatacaac ttaatatggt attcagggca acatctaaat 7800
gttgcgaatg gattttataa tttgatggaa gaagcagtca aaatggattt tgactacttc 7860
gctttctgtg atcaggatga tgtttgggat acagataaac tttccatcgg agtttctgca 7920
atttgtagtt ttaatgaacc tgctttatat tattgtggtc aaagattggc cgacggtaat 7980
ttaaatttta tagcaaatca tgaactcaac acagaacgga cactccatac aaggtttatc 8040
ctttctgatt ttgctggttg tacaggagtt ttcaataaat cgttattaaa ggaagttgtt 8100
agctatagac cgaagtatat gttgatgcat gatacatgga tattaaaagt ctgtttggct 8160
ctaggtggac aggttattat tgatacaaga ccgcatatgt attataggca acatggtggt 8220
aatacagttg gtcttggaag aagtctatct gcatacttca aacaggtaaa acagtatata 8280
actgaatata aagtggaacc acagatgaag gaacttctct taggatatgg tgatagaatt 8340
atttcggagt atgctgtaat tgcaaatgct tgttgtaatt ataaaacgga tcgtgaggca 8400
cgtacttttt tattaaatag aaataatgtt gatttttgca cgatgggatt aaatatcacc 8460
tttaaattaa aagttctatt aaataaatta taatttatta ggacgttggt attggaaata 8520
ttttaatatt acctatattc ttattacaat aggaaagtta tttgcgattt taatataaaa 8580
tttgtaggac caatgaaata tttaaaagga accttgctat ttacaatatt aaaagacagc 8640
aatccttaat ttgttaaata atttgctttt tctttgggat tattccaaag aaataagatt 8700
atatttttgt tgttaaaatt ggatatatag taaatttaaa atatcacttt aaaagtaagt 8760
aaatggagga agagtttttg ctttttagta ttataatacc tgtttttaat gtcgaaaaat 8820
atcttgagga gtgccttctc tcgattatag ggcagactga tgaaatatct gaaggatgtg 8880
aaataattct tattaatgac gggtcaacag atgatagtgc gaaaatttgt gatgagtatg 8940
agcgaaaata tcctgaattg ataaaggtat ttcataggtc aaatcatggt ttgcttctta 9000
cacgaaggtt tggatatcaa catgccgtag gaagatacat aattaattgt gactcagatg 9060
attcgttgga accgattgca cttgaggtgc tcaaagacgc aatcgtcgaa tacaacaacc 9120
cagatgttat tatatttaac cacaatacat ataaaaatgg tgtaaaggag attgcataca 9180
ataacatttt tacagagtct gtttctgccc agatagataa gctagatgtg ttgaaagagt 9240
ttctgactgg aaatcgtatt aatagcgttt gtggtgcaat ttgtaaaaag gattgtattg 9300
atataaatag agactataat aaatataaag gtcttaataa tggagaagat agccttcaaa 9360
agattgaaca attcaaaagt gcgaacacgt ttgcatatat aaataaacct ctctataatt 9420
atagggcagg atcgggtatg accacaaaat ttgatcgaaa ttattttaaa tcttttagaa 9480
tagttctgga tgaaattcaa aaagaacgag aaatgtggga ttttcctgat gtagatagat 9540
atttttcaat taaagttttg tctattgctg gaagagcaat cacacagtca agatacaatt 9600
gttgggagaa taaaagcgag caaataagat acttgcatga tattcgtaat gatgactact 9660
ttaaaaaagc agtgcttcaa ttggctacaa taaggaaata tcttcagaaa gaccatattt 9720
tactaataaa gatgttggat aagggtttga ttcgtcttat agttttgatg ttggatatta 9780
aaaataagat aggctgagtt tgagagggaa agtttttaga ggataaatga atagtataga 9840
taagaagtat cgtattaaac ttgatacgtt aatatttttg atttttatta tgttagcaac 9900
tttcaactat tcgatggagt tatcaatagt tggaaatcca ttcaggcaag tgattcttgt 9960
actgatgctc atattattgt tgtttacaat catccttaag aaatacacaa taaaaaaaat 10020
gattgtattt attacggcta tagtgtatgg attagttaac tatcgagttt caggttatac 10080
agacttattt attttattgc ttgcggcgta tgttgcggat caagtagatt ttaataacgt 10140
gttaaaagtt ctattttggg aaaaattagt gatattttta tcgcttaata tattttcagt 10200
gcttggtgta atagagatga ctaaattctc catcaataag tatctatcca ttgtagaagc 10260
atatgctcca ggatatgttt cttcgaatgt atatggctgc caagcaggag ttttattttt 10320
actttattta tcaattaatc ggtataaatt gacaaagtta aaagttctat tagtttggtt 10380
tttgagtgtt ttagtataca ttatttgtag atcaaggaca gagctaatct tagtatcagt 10440
aacaacagtt cttttattaa tatgtaataa tccaaaaaac tttaataaag ttaagaggat 10500
actctcgtgg ggatatccat ctattttggt tttaaatttt gggttaatat atactttttc 10560
aatactcggt tacggtaatc ccattatgag cactgtaaat gatgtattat tcaatgggag 10620
aattggcttg gctctcatga attttaatac ttatggtatt tcattatttg gctccccaat 10680
tgatgtttcg atagtagcta aaacgaatag atattatgct ttagataatg gttatacagt 10740
tctcgtttta tattacggcc taataggatt attatggtat tcatatattc aaattcagac 10800
tgcaaaaaaa ttagagaaga taaatgagtt ggtcctgatg gttgtactct ttatcatcaa 10860
tatctggggg atttatgaag gaaacatggt ttctttaggt ggaaatttca tgattatagc 10920
ttttctttct aaagtaacag ataattattc agaagaatta aaaagttagt agggagctag 10980
tatatgatac caaaggttat tcactattgt tggtttggag gaaaacctct accagaagat 11040
gcaaaaatgt atatcgagtc atggaaaaaa tatttaccga attatgagat aaaagagtgg 11100
aatgaaacaa actttgattt gaattgttgc gattatgtta aagaggcata tcaagaaaag 11160
aaatgggctt ttgtttcgga ttatgcaaga ctatggatta tttataatta cggtggaatt 11220
tattttgata ctgatgtcga gctaataagt tctcctgaac caatcataga aaatggagct 11280
tttttaggat gtgaactcaa tttcgaaaag gaattatcag gtgatactaa ccaaactgaa 11340
cttgttaacc caggtctagg ttttggtgcg gaaccgaaga aggattttta tcgtgagcta 11400
ttaaatttat acgaatcaca acactttatt gataaaaatg gagacctcaa tttggaaaca 11460
atagtagtga aaacaactag actgttgaag gggcatggtt ttacaggtaa tgattctatt 11520
gaacgagttg caggaattaa tatttactct catgaatatt tttgcccaat taactatcgt 11580
acaggtagaa tttcagtcac tgaaaaaact gtatctatac accattacca agaaagttgg 11640
ctttcggctt tagataagat tattaataag atagaacaga gtagaagtgg agttggaact 11700
cttgaataca aagtcagaag aattgtttct aagccattta gaggagttaa tcgtctgaag 11760
aagaagggaa ttttgggatt tctaaaaaaa tgataagact gtggtattct taataggtct 11820
cataagttct tcctaatgat ggtttggttg cttttcatta tagttcttat tggacgtttt 11880
gtctgcattc aaaaagctct ataatctcta cagtagtttt acccactata gaaattatag 11940
agccgaaaaa aatactgtcc aaggctaagg tgcctatcaa atcaaaaatg tccatagaag 12000
aagcgtattc gcatttaaat gcttgtagtc cgagacccaa tgggacttgt tatactatga 12060
acaaaataga aaatgcatat gatttgcaaa ttatcattgc ggcctataat accgaacaat 12120
atataaaaga ttgcttgaat tctgtctgtg gtcaacattc aaaatataaa actttggtga 12180
cagttataaa tgatggttca acagatggaa cagagcatat acttgctggt ataacttcgg 12240
aaaatagggg tggaagcgag ctatgcatca gagttataaa tcaggacaac cgagggcttt 12300
cgggtgccag aaatgctgct ctttctaata ttgaagcaaa gtatgtaatg tttttagact 12360
cagatgacat tctcccagag aatactattt ctgttatgtt agataaagca tttataactg 12420
atgcagatat tttacaagaa agttggtatt cgtttgatga tgaaaatgtg agtgaaaaca 12480
ttctggagga aaaagttttt gaagttggaa caaaaggata tgtttcggga tatccttggg 12540
gtaaactata caaaagttct gtattaaaaa atttttaatt tccaaaagga tactggtttg 12600
aagatacacc aatcactttt attcttgcag caatgccaat taaagtggta accataaagg 12660
atattgttta tggctatcga ctgaatccaa atggaattac agcaaagtca atctttcata 12720
agaaatcagt tgatactttt tgggttacgg aattatgttt gaacgaacta ccaagattca 12780
aagtgtcata tgatcagcaa tcatatgaat atttattaag acaatcattg atgaatgaag 12840
ccaggataaa gaatcaggga aagaaaatac gagaagcagt atttgtcctg acatcagaat 12900
taatgaaaat ttattttagt ggtttttctt caaaagattc aaagatgaag aaaatagaaa 12960
tagcacttag aaatagacaa tttatccagt ttgaattatc aaaactgtat ttgtaatgat 13020
agattaagtg agacagggag aacagttagt gaatagatac agatatttgt taaaaaacat 13080
aggccttttg accctaagta gtttttcaac caaattactt agtttctttt tggtaccgtt 13140
atataccaat atattatcga ctactgaata tggaacctat gatttattta atactacaat 13200
aggtgtatta ctcccgatcc tcacattgaa tatacaggaa gcggtgatga gattttccat 13260
tgacagtaag tatgatagaa aatcaatcgt gacagtgaca gcaaggttct ttattctttc 13320
aaatttaatt gttattcttg ggctttgggt aaattataca tttgtattta gcgtgattgc 13380
caagcagtat gccatatttt tcttcctgat gtttttatcg cagtcgctat ccgggatgat 13440
aaccatgtat gtcagaggaa tagacaaaat atctgacttg tctttttcaa gtgtaatagc 13500
atctgttatt actatatgct taaatgtttc gttcttagca tttcttcact ggggacttgt 13560
tggatatttt atggccaata taatcggccc gatggttcaa tcactttatt tgattgttaa 13620
agcacatatt ttaggggata ttcatttaga gcaagcatat cagtctgaaa aaaatgaaat 13680
ggttaattat agtaaaccat tgattgcaaa cagcatcgcg tggtgggtaa ataatttttc 13740
tgacagatat atcgttgtga ttttttgtgg gttggctgaa aatggaatat attcagtggc 13800
atcgaaaatt ccttcgatac ttaatatttt tcagacaatt tttaatcagg cgtggacgct 13860
gtctgctgta aaggatttcg accctgagga taaaaacggc tttttcacca atacatataa 13920
agcatataat tgcatgatgg ttgttctatg ctctggaatt attgtattta acaaactgtt 13980
agccagtttt ttgtatgcaa aagactttta tgttgcttgg aaatacgtac catggttaac 14040
aattgcaatc gtattcggag caatgtccgg atatattgga ggaatttttg ctgctgtcaa 14100
ggattctaaa atatttgcga aatcaactgt ttgtggagcg ataactaatg taatattaaa 14160
tttaatactt acacccatta tgggaccatt gggagctgct attgctacgg cagtatccta 14220
ttttgaggta tggattttta gatatttgca atccaggaga tatattagga ttagggttaa 14280
tatatttagg gatcttatta cttatttctt gctgttttgt cagtcgatta tacttttgat 14340
tgaaatggaa aattcttatt tatatgttct tgaaatcgga atttttattt tgatagtttt 14400
gctatatttt aaggatattc ttttgctatt gaacaaaggt agtaattcaa tcaaaatgaa 14460
gaaaagggga agtgaattat agtatgaaga tagggataat aacgtggttt acaggctcta 14520
attatggcac taaccttcag gcaatcgctc ttcaatatta cttgagaagt cgtggttatg 14580
ggctctttgt caactgtagt gggtgacgaa aagctaacat ctagagagga ccggataggt 14640
ccttttttta tgtatgttca gtgtgatgaa gacacgcttc ttaaagttga taaagtttct 14700
aaaaccgaag cccaagcgtt tgatgtcttt gatcaactta ttagtcgctt caagtttcgc 14760
gtttgaatag tccgtttcta gtgcgttttt gatgtattgc ttgtgtctaa gaaaagtcct 14820
aaagacagtt tgaaaataat gattgacctt gctcctattt tcctctatca gttcaaagaa 14880
ctcatctact ctcttctcct gaaagtgaaa aaacaaaagc tgataaagtg tatagtagtc 14940
agtaagctct tttgaaaaga ctagtgtctt cgcaacaact tcatgtggtg ctaaagtttg 15000
gcggaaagtc tttgaataaa aagaattgag agatagttta cagctgtcct tttggaagag 15060
tcgccagtga tttttcaagg ctcgataggg tagtgacttc ttatcgaact ggttcatgat 15120
tgcaattctt gtctttaaaa aggcaagtcc aaggtgctgg atgatgtgga aacgatcaag 15180
aacgattttg acgtttggaa agagtctgcg ggctagtggg atataggctc cagacatatc 15240
cattgtgata aactgtacct gttgtcggac tttcaatgga tacttcaaaa agtagtttcg 15300
tatagtagtt tggcggcgat tatcaaggat ggttatgagt tcgtttgtct cataattctg 15360
cgccacaaaa gccaattccc ctttcttgaa cccaaactca tcccaggaca taacagcagg 15420
gagtttgtca taatgttcct tgaaagtaaa ctgatcaagc ttacgataga cagtggacgt 15480
cgacacgcga agtcttcttg caatatcagt tagtgacact ttctcagtta ggagttgtgt 15540
aactttttgt cggactagat tggagatttg gcagtttttc tcaacgatag atgtctcagc 15600
cacccttact ctcctacaac ttttacactg gaaacgacgt tttttcagac gtagtagagt 15660
tggcgttccc gcttgctcga gaagagagat tttagagttt ttttgaaagt catacttgat 15720
catctttcca tggcaatgag gacatgatgg tgaagggtaa tcaagttttg cttgaatctc 15780
gatatgagtg tcagtttcaa aaacaagtga aatcttgata ttttggtctt tgattccgat 15840
taattctgtg ttattcttaa taagtttcat aagttcttcc taatgatggt ttggttgctt 15900
ttcattatag ttcttatggg actttttgtg tacactcaaa aagctctata atccctacag 15960
tagttttacc cactacagaa attatagagc caatatatct cctgtctatt tttatgctac 16020
ttttgggtta gc 16032
<210>2
<211>906
<212>DNA
<213>Streptococcus thermophilus
<400>2
aaggagagat tcgtgaaacc taaaatactt gtacttatgg caacctataa tggaggaaaa 60
tacttacgag agcaattaga tagtgttttt ttacaaaaaa atgtggatat tactgtcctg 120
gttcgtgacg atggttcaat tgacaatacg tgtgctattc ttgatgaata tagtaagaga 180
tacaacttaa tatggtattc agggcaacat ctaaatgttg cgaatggatt ttataatttg 240
atggaagaag cagtcaaaat ggattttgac tacttcgctt tctgtgatca ggatgatgtt 300
tgggatacag ataaactttc catcggagtt tctgcaattt gtagttttaa tgaacctgct 360
ttatattatt gtggtcaaag attggccgac ggtaatttaa attttatagc aaatcatgaa 420
ctcaacacag aacggacact ccatacaagg tttatccttt ctgattttgc tggttgtaca 480
ggagttttca ataaatcgtt attaaaggaa gttgttagct atagaccgaa gtatatgttg 540
atgcatgata catggatatt aaaagtctgt ttggctctag gtggacaggt tattattgat 600
acaagaccgc atatgtatta taggcaacat ggtggtaata cagttggtct tggaagaagt 660
ctatctgcat acttcaaaca ggtaaaacag tatataactg aatataaagt ggaaccacag 720
atgaaggaac ttctcttagg atatggtgat agaattattt cggagtatgc tgtaattgca 780
aatgcttgtt gtaattataa aacggatcgt gaggcacgta cttttttatt aaatagaaat 840
aatgttgatt tttgcacgat gggattaaat atcaccttta aattaaaagt tctattaaat 900
aaatta 906
<210>3
<211>1032
<212>DNA
<213>Streptococcus thermophilus
<400>3
atggaggaag agtttttgct ttttagtatt ataatacctg tttttaatgt cgaaaaatat 60
cttgaggagt gccttctctc gattataggg cagactgatg aaatatctga aggatgtgaa 120
ataattctta ttaatgacgg gtcaacagat gatagtgcga aaatttgtga tgagtatgag 180
cgaaaatatc ctgaattgat aaaggtattt cataggtcaa atcatggttt gcttcttaca 240
cgaaggtttg gatatcaaca tgccgtagga agatacataa ttaattgtga ctcagatgat 300
tcgttggaac cgattgcact tgaggtgctc aaagacgcaa tcgtcgaata caacaaccca 360
gatgttatta tatttaacca caatacatat aaaaatggtg taaaggagat tgcatacaat 420
aacattttta cagagtctgt ttctgcccag atagataagc tagatgtgtt gaaagagttt 480
ctgactggaa atcgtattaa tagcgtttgt ggtgcaattt gtaaaaagga ttgtattgat 540
ataaatagag actataataa atataaaggt cttaataatg gagaagatag ccttcaaaag 600
attgaacaat tcaaaagtgc gaacacgttt gcatatataa ataaacctct ctataattat 660
agggcaggat cgggtatgac cacaaaattt gatcgaaatt attttaaatc ttttagaata 720
gttctggatg aaattcaaaa agaacgagaa atgtgggatt ttcctgatgt agatagatat 780
ttttcaatta aagttttgtc tattgctgga agagcaatca cacagtcaag atacaattgt 840
tgggagaata aaagcgagca aataagatac ttgcatgata ttcgtaatga tgactacttt 900
aaaaaagcag tgcttcaatt ggctacaata aggaaatatc ttcagaaaga ccatatttta 960
ctaataaaga tgttggataa gggtttgatt cgtcttatag ttttgatgtt ggatattaaa 1020
aataagatag gc 1032
<210>4
<211>1140
<212>DNA
<213>Streptococcus thermophilus
<400>4
atgaatagta tagataagaa gtatcgtatt aaacttgata cgttaatatt tttgattttt 60
attatgttag caactttcaa ctattcgatg gagttatcaa tagttggaaa tccattcagg 120
caagtgattc ttgtactgat gctcatatta ttgttgttta caatcatcct taagaaatac 180
acaataaaaa aaatgattgt atttattacg gctatagtgt atggattagt taactatcga 240
gtttcaggtt atacagactt atttatttta ttgcttgcgg cgtatgttgc ggatcaagta 300
gattttaata acgtgttaaa agttctattt tgggaaaaat tagtgatatt tttatcgctt 360
aatatatttt cagtgcttgg tgtaatagag atgactaaat tctccatcaa taagtatcta 420
tccattgtag aagcatatgc tccaggatat gtttcttcga atgtatatgg ctgccaagca 480
ggagttttat ttttacttta tttatcaatt aatcggtata aattgacaaa gttaaaagtt 540
ctattagttt ggtttttgag tgttttagta tacattattt gtagatcaag gacagagcta 600
atcttagtat cagtaacaac agttctttta ttaatatgta ataatccaaa aaactttaat 660
aaagttaaga ggatactctc gtggggatat ccatctattt tggttttaaa ttttgggtta 720
atatatactt tttcaatact cggttacggt aatcccatta tgagcactgt aaatgatgta 780
ttattcaatg ggagaattgg cttggctctc atgaatttta atacttatgg tatttcatta 840
tttggctccc caattgatgt ttcgatagta gctaaaacga atagatatta tgctttagat 900
aatggttata cagttctcgt tttatattac ggcctaatag gattattatg gtattcatat 960
attcaaattc agactgcaaa aaaattagag aagataaatg agttggtcct gatggttgta 1020
ctctttatca tcaatatctg ggggatttat gaaggaaaca tggtttcttt aggtggaaat 1080
ttcatgatta tagcttttct ttctaaagta acagataatt attcagaaga attaaaaagt 1140
<210>5
<211>810
<212>DNA
<213>Streptococcus thermophilus
<400>5
tatatgatac caaaggttat tcactattgt tggtttggag gaaaacctct accagaagat 60
gcaaaaatgt atatcgagtc atggaaaaaa tatttaccga attatgagat aaaagagtgg 120
aatgaaacaa actttgattt gaattgttgc gattatgtta aagaggcata tcaagaaaag 180
aaatgggctt ttgtttcgga ttatgcaaga ctatggatta tttataatta cggtggaatt 240
tattttgata ctgatgtcga gctaataagt tctcctgaac caatcataga aaatggagct 300
tttttaggat gtgaactcaa tttcgaaaag gaattatcag gtgatactaa ccaaactgaa 360
cttgttaacc caggtctagg ttttggtgcg gaaccgaaga aggattttta tcgtgagcta 420
ttaaatttat acgaatcaca acactttatt gataaaaatg gagacctcaa tttggaaaca 480
atagtagtga aaacaactag actgttgaag gggcatggtt ttacaggtaa tgattctatt 540
gaacgagttg caggaattaa tatttactct catgaatatt tttgcccaat taactatcgt 600
acaggtagaa tttcagtcac tgaaaaaact gtatctatac accattacca agaaagttgg 660
ctttcggctt tagataagat tattaataag atagaacaga gtagaagtgg agttggaact 720
cttgaataca aagtcagaag aattgtttct aagccattta gaggagttaa tcgtctgaag 780
aagaagggaa ttttgggatt tctaaaaaaa 810
<210>6
<211>750
<212>DNA
<213>Streptococcus thermophilus
<400>6
gttcttccta atgatggttt ggttgctttt cattatagtt cttattggac gttttgtctg 60
cattcaaaaa gctctataat ctctacagta gttttaccca ctatagaaat tatagagccg 120
aaaaaaatac tgtccaaggc taaggtgcct atcaaatcaa aaatgtccat agaagaagcg 180
tattcgcatt taaatgcttg tagtccgaga cccaatggga cttgttatac tatgaacaaa 240
atagaaaatg catatgattt gcaaattatc attgcggcct ataataccga acaatatata 300
aaagattgct tgaattctgt ctgtggtcaa cattcaaaat ataaaacttt ggtgacagtt 360
ataaatgatg gttcaacaga tggaacagag catatacttg ctggtataac ttcggaaaat 420
aggggtggaa gcgagctatg catcagagtt ataaatcagg acaaccgagg gctttcgggt 480
gccagaaatg ctgctctttc taatattgaa gcaaagtatg taatgttttt agactcagat 540
gacattctcc cagagaatac tatttctgtt atgttagata aagcatttat aactgatgca 600
gatattttac aagaaagttg gtattcgttt gatgatgaaa atgtgagtga aaacattctg 660
gaggaaaaag tttttgaagt tggaacaaaa ggatatgttt cgggatatcc ttggggtaaa 720
ctatacaaaa gttctgtatt aaaaaatttt 750
<210>7
<211>435
<212>DNA
<213>Streptococcus thermophilus
<400>7
tttccaaaag gatactggtt tgaagataca ccaatcactt ttattcttgc agcaatgcca 60
attaaagtgg taaccataaa ggatattgtt tatggctatc gactgaatcc aaatggaatt 120
acagcaaagt caatctttca taagaaatca gttgatactt tttgggttac ggaattatgt 180
ttgaacgaac taccaagatt caaagtgtca tatgatcagc aatcatatga atatttatta 240
agacaatcat tgatgaatga agccaggata aagaatcagg gaaagaaaat acgagaagca 300
gtatttgtcc tgacatcaga attaatgaaa atttatttta gtggtttttc ttcaaaagat 360
tcaaagatga agaaaataga aatagcactt agaaatagac aatttatcca gtttgaatta 420
tcaaaactgt atttg 435
<210>8
<211>1452
<212>DNA
<213>Streptococcus thermophilus
<400>8
gtgagacagg gagaacagtt agtgaataga tacagatatt tgttaaaaaa cataggcctt 60
ttgaccctaa gtagtttttc aaccaaatta cttagtttct ttttggtacc gttatatacc 120
aatatattat cgactactga atatggaacc tatgatttat ttaatactac aataggtgta 180
ttactcccga tcctcacatt gaatatacag gaagcggtga tgagattttc cattgacagt 240
aagtatgata gaaaatcaat cgtgacagtg acagcaaggt tctttattct ttcaaattta 300
attgttattc ttgggctttg ggtaaattat acatttgtat ttagcgtgat tgccaagcag 360
tatgccatat ttttcttcct gatgttttta tcgcagtcgc tatccgggat gataaccatg 420
tatgtcagag gaatagacaa aatatctgac ttgtcttttt caagtgtaat agcatctgtt 480
attactatat gcttaaatgt ttcgttctta gcatttcttc actggggact tgttggatat 540
tttatggcca atataatcgg cccgatggtt caatcacttt atttgattgt taaagcacat 600
attttagggg atattcattt agagcaagca tatcagtctg aaaaaaatga aatggttaat 660
tatagtaaac cattgattgc aaacagcatc gcgtggtggg taaataattt ttctgacaga 720
tatatcgttg tgattttttg tgggttggct gaaaatggaa tatattcagt ggcatcgaaa 780
attccttcga tacttaatat ttttcagaca atttttaatc aggcgtggac gctgtctgct 840
gtaaaggatt tcgaccctga ggataaaaac ggctttttca ccaatacata taaagcatat 900
aattgcatga tggttgttct atgctctgga attattgtat ttaacaaact gttagccagt 960
tttttgtatg caaaagactt ttatgttgct tggaaatacg taccatggtt aacaattgca 1020
atcgtattcg gagcaatgtc cggatatatt ggaggaattt ttgctgctgt caaggattct 1080
aaaatatttg cgaaatcaac tgtttgtgga gcgataacta atgtaatatt aaatttaata 1140
cttacaccca ttatgggacc attgggagct gctattgcta cggcagtatc ctattttgag 1200
gtatggattt ttagatattt gcaatccagg agatatatta ggattagggt taatatattt 1260
agggatctta ttacttattt cttgctgttt tgtcagtcga ttatactttt gattgaaatg 1320
gaaaattctt atttatatgt tcttgaaatc ggaattttta ttttgatagt tttgctatat 1380
tttaaggata ttcttttgct attgaacaaa ggtagtaatt caatcaaaat gaagaaaagg 1440
ggaagtgaat ta 1452
序列表
<110>罗迪亚化学
<120>组织形成乳酸细菌
<130>0910613
<150>FR 0303242
<151>2003-03-17
<160>8
<170>PatentIn version 3.2
<210>1
<211>16032
<212>DNA
<213>Streptococcus thermophilus
<400>1
tttgtaaaag gacgccattt ggtcgtcctt ttgtgttgta gctaatatct gttcgaagtg 60
ataataagtt aaaatttttc aaactactag aaaaaataaa aatatttgga agaagaagac 120
ttttaataaa taggtaaata tctgacaatt taaagtttaa ctactaaaaa tgtgaaagat 180
agttcacaat ataatggaaa atgatataaa ttaaatgatt gatatcataa tgaaaacgtt 240
tttttgtttt tttttgaaaa aaatgacaat tgaaatgaaa ttgtattaat gttacaataa 300
taatggggaa tacttaattt taatttttag gagcaattta tatgagttcg cgtacgaatc 360
gtaagcaaaa acgtacgggt aatagatcat gggggatggt caacgttgga ttgaccattc 420
tgtatgctat tttagcattg gtcttattat tcaccatgtt caattataat ttcctatcct 480
ttaggttttt gaacatcatt atcactattg gtttgttggt agttcttgct attagcatct 540
tccttcagaa gactaagaaa tcaccactag tgacaacggt tgtattggtt atcttctcgc 600
tagtttctct ggttggtatt tttggtttta aacaaatgat tgacatcact aaccgtatga 660
atcagacggc agcattttct gaagtagaaa tgagcatcgt ggttcctaag gaaagtgaca 720
tcaaagatgt gagccagctt actagcgtac aggcacctac taaggttgat aagaacaata 780
tcgagatctt gatgtcagct ctcaaaaaag ataaaaaagt tgatgttaaa gttgatgatg 840
ttgcctcata tcaagaagct tatgataatc ttaagtctgg caaatctaaa gctatggtct 900
tgagtgggtc ttatgctagc ctactagagt ctgtcgatag taattatgct tcaaatctaa 960
aaacaattta tacttataaa attaaaaaga agaatagcaa ctctgcaaac caagtagatt 1020
caaaagtctt caatatttat attagtggta ttgataccta cggttcgatt tcaacagtgt 1080
cacgttcaga tgtcaatatt attatgacag taaacatgaa tacacataag attctcttga 1140
cgactactcc acgtgatgca tacgttaaga ttcctggtgg tggagcagac cagtatgata 1200
aattaaccca cgcaggtatt tatggcgttg aaacatctga acaaactctg gaagatctat 1260
atggtactaa gattgattac tatgcccgaa ttaacttcac atctttcctt aagttgattg 1320
accaacttgg tggtgtgaca gtccataatg atcaagcttt cacaagtctt catgggaagt 1380
ttgatttccc agttggagat atccaaatga attcagagca agcacttgga tttgttcgtg 1440
aacgctatag tttagatggc ggagataatg atcgtggtaa aaaccaggaa aaagtcattt 1500
ctgcgattgt aaacaagttg gcttctctaa agtctgtatc aaactttact tcaatcgtta 1560
ataatctcca agactctgtt cagacaaata tttctttgga taccattaat gctttggcta 1620
atacacaact tgattcaggt tctaaattta cggtgacttc tcaagcagta actggtacag 1680
gttcaaccgg acaattgacc tcttatgcga tgccaaattc tagtctttac atgatgaaac 1740
tagataattc gagtgtggca agtgcctctc aagctatcaa aaatctaatg gaggaaaaat 1800
aagtgattga cgttcactca catattgttt ttgatgttga tgatggtcct aaaactttag 1860
aagaaagttt agatctcatt ggtgaaagtt acgcccaggg ggtacgtaag attgtttcaa 1920
catcccatcg tcgtaagggg atgtttgaga ctccagagga taaaattttt gccaactttt 1980
ctaaggtaaa agcagaagca gaagcacttt atccagactt aactatttat tatggaggtg 2040
aactttatta caccttagac attgtggaga aacttgaaaa gaatctcatt ccgcgcatgc 2100
acaacactca atttgctttg attgagttta gtgctcgcac atcttggaaa gaaattcata 2160
gtgggcttag taatgttttg agagcggggg taacgcctat tgttgctcat attgagcgct 2220
atgatgccct cgaagaaaat gctgaccgtg ttcgagaaat catcaacatg gggtgctata 2280
ctcaagttaa tagctctcat gttctaaaac caaagctctt tggagataaa gataaagtaa 2340
gaaaaaaacg tgttcgttat tttttggaga aaaatttggt tcatatggtt gctagcgaca 2400
tgcataatct tgggccgaga ccaccattta tgaaagatgc ttatgaaatt gttaaaaaga 2460
actacggccc caaacgtgct aagaatcttt ttattgaaaa tcccaaaaca ttactagaaa 2520
atcaatattt ataggagata ttatgaatca agataacact aaaagtgatg aaatcgacgt 2580
actagcattg ctacataaac tttggacgaa gaagcttttg attcttttca cagcttttta 2640
tttcgctgct ttcagtttct taggtactta tttctttatc caaccaacat atacatcaac 2700
aacgcgtatc tatgttgtta atcaggcaac agataataat aatctttctg ctcaagattt 2760
gcaagctggt acctatttgg caaatgacta taaagagttt attacatcaa atgatgtatt 2820
atcagaagtt attaaagatg aaaaattgaa tttgagtgag gcagaactgt ctaaaatggt 2880
ttcagttaat attcctactg atactcgtct tatttcaatt tctgttaatg ctaaaactgg 2940
tcaagatgcg caaacacttg ctaataaggt tcgtgaagtt gcttcaaaaa aaatcaagaa 3000
ggtgacaaaa gttgaagatg tcacaacgct cgaagaagct aaattgccag agtcaccatc 3060
ttcaccaaat atcaaacgta atgtgcttct tggggcagtg cttggaggat tccttgcagt 3120
ggttggtgta ttggtacgtg aaatcctaga tgatcgtgtt cgccgtccag aagatgtgga 3180
agatgccctt ggaatgacac ttcttggaat tgtccctgat acagataaga tttaaggaga 3240
agaaatgcct ttattaaagt tagttaaatc aaaagtagac tttgctaaaa agacggaaga 3300
gtattataac gctattcgca caaatattca attttctggt gctcagatta aagtgattgc 3360
gattagctct gttgaaactg gtgaaggaaa atcaacgaca tctgttaact tggcgatttc 3420
atttgctagt gttgggctcc gaacacttct gattgatgcg gatacgcgta attctgtttt 3480
gtcgggtaca tttaaatcaa atgagcctta taaaggtctt tcaaatttcc tttcaggaaa 3540
tgccgatcta aatgaaacga tttgccaaac tgatatttct ggtttagatg ttattgcatc 3600
tggtcctgta ccacctaatc caacaagtct tttgcaaaat gacaatttta gacatttgat 3660
ggaagttgct cgtagtcgtt atgattatgt tatcatttat acaccaccaa ttggtctggt 3720
cattgatgct ggtattattg cccatcaggc tgatgctagt cttttggtta cagcagctgg 3780
aaaaattaaa cgtcgtttcg taactaaggc ggtcgaacaa ttgaaacaaa gtggttctca 3840
gttcttaggt gtcgtcctta ataaagttga catgacagtt gataaatatg gatcatatgg 3900
ttcttacgga tcatatggtg agtacgggaa aaaaacagac caaaaagaag gtcattcaag 3960
agcacatcgt cgtagaaaag gatagcatta atggggatga tgcggctcct tataccttaa 4020
cagattaaaa aggggtttag agtgaaagaa aaacaagaaa ttcgtcgcat tgaaattggt 4080
attatacagt tggttgtggt tgttttcgca gccatggtag ctagtaaaat accatataca 4140
gagattaccc aaggaagtat tgtcctttta ggtgtcgtac atgtagtgtc ttactatatc 4200
agtagttatt atgaaaatct taagtataga ggctacttgg atgaactcat tgcaactgtc 4260
aaatattgtt tcatatttgc tctaattgca acatttctct cgttttttgc agatggaagt 4320
ttttcaatct cacgtcgcgg acttctttac gtcaccatga tttcaggtgt tctcttatac 4380
gttacaaata ctgttcttaa gtatttccgc tcatctattt atacacgtcg taaaagtaac 4440
aagaatattc tcttgatttc tgatcaggca cgtcttgata atgttttatc tcgtatgaag 4500
gacaatatgg atggtaggat ttcagcagtt tgtgtcttgg ataatcctta tttcactgat 4560
ccatttatca agagtgttaa acctgaaaat ttgattgaat atgcgacaca ctcagtagta 4620
gaccaagttt tgattaatct gccaagtgag cagtacaaga tttgggatta tgcgtcacca 4680
tttgaactta tgggaatccc agtatccatt aatttgaatg cccttgaatt tatgagtcag 4740
ggtgaaaaac gtatccaaca attgggtcct ttcaaagttg ttacgttttc tacgcatttt 4800
tatagctatg gagatatctt ggcgaaacgc ttcctcgata tctgtggagc tctagttggt 4860
ttggtgctct gtgggattgt tggaatcttc ctttatccac ttattcgtaa ggatggtggg 4920
ccagccattt ttgctcaaga ccgtgtggga gaaaatggac gtatcttcaa gttttataaa 4980
ttccgttcta tgcgtgttga tgctgaggaa attaaaaagc aattgatgga taaaaatcaa 5040
atgtctggtg gtatgtttaa gatagacaat gatccacgta ttaccaaaat tggacatttc 5100
attcgtaaaa caagtcttga tgaacttcca caattttgga atgttctaaa aggtgatatg 5160
agcttggtag gaacacgtcc accaacattg gatgagtacg aatcttatac accggaacaa 5220
aaacgtcgtt taagttttaa acctggtatc acaggtcttt ggcaagtaag tggacgaagt 5280
gaaattactg attttgatga agttgtaaaa ctagatgttg cttatatgga tgggtggaca 5340
atctggcgag atatcaaaat cttattgaaa acgattaaag tagtagtaat gaaggatgga 5400
gcaaaatgat aacttcaaag atgattagat gagaaaaata atttatattg ttgcttctaa 5460
agggattcct gcaaaatatg gtggatttga gacctttgtt gagaagttga cagagttcca 5520
ataagacaaa gatatccaat attatgtagc ttgtatgcgt gaaaactctg caaaatcagg 5580
aattagagca gatactttat caactgcact gtgcgtagat acaaatatct attccttatg 5640
actgctgaac aacagaagaa tacattagtc attaccacca ctgagagatt gcgattaatg 5700
gaggggctct ttgagtcaga tcaacttcct atcaaatatt tggcaggtat agtagtcatc 5760
ggagatggtg aagtggcgtt tccagaggga gtcccaataa ttccttttga tgatgcgatt 5820
gactttgcga ctcatgaagt tgtcgaccat gtgtttatca acttaccgag tgaacattac 5880
gatctcaaac atcttgtttc cgattttgaa gtcatgggta ttgatgtgag tgtagatatt 5940
aacttatttg atttcagggc tttaaaaaat aaaaaaatca aacaagttgg agaccatagt 6000
atcgtgactt ttaactccaa ttactacaaa catagccata tctttctaaa gcgcatgttg 6060
gatatctttg gggctgtgat tggtcttcta atttgtggtc tggtcgggat tgtcttagct 6120
cctatcattc ggaaagatgg aggaacctgc tattttcgtt cagaaacgag tagggaaaaa 6180
cggtcgtatc tttaattttt ataaattccg ttctatgtat atagatgcag aaaaacggaa 6240
aaaagaactt atggctcaaa accagatgca gggtggtatg tttaaaatgg ataatgaccc 6300
acggattaca ccaattggtc agtttatccg aaaaacaagt ctggatgaat tgccacagtt 6360
ttataatgta ctagttggtg atatgagctt ggtaggtact cgtccaccga cagtggatga 6420
atttgaaaag tatacaccaa gtcaaaaacg acgacttaat tttaaaccgg ggattaccgg 6480
cttatggcaa gttagtgggc gtagcaatat tacggacttt gacgaagtag ttaaactcga 6540
tgttgaatac atagataact ggtcaatttg gtcggatatc aagatattgt tgaagactat 6600
cttcgtagta tttaaaaaag agggaagtaa gtagagtata tcatgaaagt ttgtttagta 6660
ggttcttctg gtgggcattt ggcacacttg aatatgctta aacctttttg gagtgaacag 6720
gaccgctttt gggttacctt tgataaagaa gatgcaagaa gtattttaaa agatgagcag 6780
ttttatccgt gctattttcc tactaacaga aattttaaaa atttagtaaa gaatactttt 6840
ttagcactta aaattttaag aaaagaaaga cctgacgtta ttatttcatc aggagcagcg 6900
gtagcagttc cgttttttta tcttggtaaa ctgtttggag cgaaaacggt ttatatagaa 6960
gtatttgata gaatagataa accgacagta actgggaaat tggtttatcc agtgacagat 7020
aaatttattg ttcagtggga ggagatgaaa actgtctatc ccaaagctat taatctgggg 7080
agtatttttt aatgattttt gttacagtgg gaacccacga acagcccttt aataggctta 7140
ttaaggaagt tgatcgttta aaaaaagaag gtattattac agatgaggtt tttattcaga 7200
caggtttttc aacttatgaa cctcaatact gtgactggaa aaaacttatc tcttattctg 7260
agatggacaa ctacatgact cactctgata tcattataac ccatggtggt ccagcgacat 7320
tcatgggagc aattgccaaa ggcaaaaaac caattgttgt tccaagacaa gaaaaatatg 7380
gagaacatgt aaatgatcat cagttagagt ttgcagaaca ggtttctgga cgttttggga 7440
gtattattgt tgtagaagat attacttcac tctgtgaaat tttattaaaa ggaagtaacg 7500
ttatttgcca aaagcagagt caattttacc ctaaaggatc acagtttatt tttaaattca 7560
aagagatagt tgatgaactt gtagaaggag agattcgtga aacctaaaat acttgtactt 7620
atggcaacct ataatggagg aaaatactta cgagagcaat tagatagtgt ttttttacaa 7680
aaaaatgtgg atattactgt cctggttcgt gacgatggtt caattgacaa tacgtgtgct 7740
attcttgatg aatatagtaa gagatacaac ttaatatggt attcagggca acatctaaat 7800
gttgcgaatg gattttataa tttgatggaa gaagcagtca aaatggattt tgactacttc 7860
gctttctgtg atcaggatga tgtttgggat acagataaac tttccatcgg agtttctgca 7920
atttgtagtt ttaatgaacc tgctttatat tattgtggtc aaagattggc cgacggtaat 7980
ttaaatttta tagcaaatca tgaactcaac acagaacgga cactccatac aaggtttatc 8040
ctttctgatt ttgctggttg tacaggagtt ttcaataaat cgttattaaa ggaagttgtt 8100
agctatagac cgaagtatat gttgatgcat gatacatgga tattaaaagt ctgtttggct 8160
ctaggtggac aggttattat tgatacaaga ccgcatatgt attataggca acatggtggt 8220
aatacagttg gtcttggaag aagtctatct gcatacttca aacaggtaaa acagtatata 8280
actgaatata aagtggaacc acagatgaag gaacttctct taggatatgg tgatagaatt 8340
atttcggagt atgctgtaat tgcaaatgct tgttgtaatt ataaaacgga tcgtgaggca 8400
cgtacttttt tattaaatag aaataatgtt gatttttgca cgatgggatt aaatatcacc 8460
tttaaattaa aagttctatt aaataaatta taatttatta ggacgttggt attggaaata 8520
ttttaatatt acctatattc ttattacaat aggaaagtta tttgcgattt taatataaaa 8580
tttgtaggac caatgaaata tttaaaagga accttgctat ttacaatatt aaaagacagc 8640
aatccttaat ttgttaaata atttgctttt tctttgggat tattccaaag aaataagatt 8700
atatttttgt tgttaaaatt ggatatatag taaatttaaa atatcacttt aaaagtaagt 8760
aaatggagga agagtttttg ctttttagta ttataatacc tgtttttaat gtcgaaaaat 8820
atcttgagga gtgccttctc tcgattatag ggcagactga tgaaatatct gaaggatgtg 8880
aaataattct tattaatgac gggtcaacag atgatagtgc gaaaatttgt gatgagtatg 8940
agcgaaaata tcctgaattg ataaaggtat ttcataggtc aaatcatggt ttgcttctta 9000
cacgaaggtt tggatatcaa catgccgtag gaagatacat aattaattgt gactcagatg 9060
attcgttgga accgattgca cttgaggtgc tcaaagacgc aatcgtcgaa tacaacaacc 9120
cagatgttat tatatttaac cacaatacat ataaaaatgg tgtaaaggag attgcataca 9180
ataacatttt tacagagtct gtttctgccc agatagataa gctagatgtg ttgaaagagt 9240
ttctgactgg aaatcgtatt aatagcgttt gtggtgcaat ttgtaaaaag gattgtattg 9300
atataaatag agactataat aaatataaag gtcttaataa tggagaagat agccttcaaa 9360
agattgaaca attcaaaagt gcgaacacgt ttgcatatat aaataaacct ctctataatt 9420
atagggcagg atcgggtatg accacaaaat ttgatcgaaa ttattttaaa tcttttagaa 9480
tagttctgga tgaaattcaa aaagaacgag aaatgtggga ttttcctgat gtagatagat 9540
atttttcaat taaagttttg tctattgctg gaagagcaat cacacagtca agatacaatt 9600
gttgggagaa taaaagcgag caaataagat acttgcatga tattcgtaat gatgactact 9660
ttaaaaaagc agtgcttcaa ttggctacaa taaggaaata tcttcagaaa gaccatattt 9720
tactaataaa gatgttggat aagggtttga ttcgtcttat agttttgatg ttggatatta 9780
aaaataagat aggctgagtt tgagagggaa agtttttaga ggataaatga atagtataga 9840
taagaagtat cgtattaaac ttgatacgtt aatatttttg atttttatta tgttagcaac 9900
tttcaactat tcgatggagt tatcaatagt tggaaatcca ttcaggcaag tgattcttgt 9960
actgatgctc atattattgt tgtttacaat catccttaag aaatacacaa taaaaaaaat 10020
gattgtattt attacggcta tagtgtatgg attagttaac tatcgagttt caggttatac 10080
agacttattt attttattgc ttgcggcgta tgttgcggat caagtagatt ttaataacgt 10140
gttaaaagtt ctattttggg aaaaattagt gatattttta tcgcttaata tattttcagt 10200
gcttggtgta atagagatga ctaaattctc catcaataag tatctatcca ttgtagaagc 10260
atatgctcca ggatatgttt cttcgaatgt atatggctgc caagcaggag ttttattttt 10320
actttattta tcaattaatc ggtataaatt gacaaagtta aaagttctat tagtttggtt 10380
tttgagtgtt ttagtataca ttatttgtag atcaaggaca gagctaatct tagtatcagt 10440
aacaacagtt cttttattaa tatgtaataa tccaaaaaac tttaataaag ttaagaggat 10500
actctcgtgg ggatatccat ctattttggt tttaaatttt gggttaatat atactttttc 10560
aatactcggt tacggtaatc ccattatgag cactgtaaat gatgtattat tcaatgggag 10620
aattggcttg gctctcatga attttaatac ttatggtatt tcattatttg gctccccaat 10680
tgatgtttcg atagtagcta aaacgaatag atattatgct ttagataatg gttatacagt 10740
tctcgtttta tattacggcc taataggatt attatggtat tcatatattc aaattcagac 10800
tgcaaaaaaa ttagagaaga taaatgagtt ggtcctgatg gttgtactct ttatcatcaa 10860
tatctggggg atttatgaag gaaacatggt ttctttaggt ggaaatttca tgattatagc 10920
ttttctttct aaagtaacag ataattattc agaagaatta aaaagttagt agggagctag 10980
tatatgatac caaaggttat tcactattgt tggtttggag gaaaacctct accagaagat 11040
gcaaaaatgt atatcgagtc atggaaaaaa tatttaccga attatgagat aaaagagtgg 11100
aatgaaacaa actttgattt gaattgttgc gattatgtta aagaggcata tcaagaaaag 11160
aaatgggctt ttgtttcgga ttatgcaaga ctatggatta tttataatta cggtggaatt 11220
tattttgata ctgatgtcga gctaataagt tctcctgaac caatcataga aaatggagct 11280
tttttaggat gtgaactcaa tttcgaaaag gaattatcag gtgatactaa ccaaactgaa 11340
cttgttaacc caggtctagg ttttggtgcg gaaccgaaga aggattttta tcgtgagcta 11400
ttaaatttat acgaatcaca acactttatt gataaaaatg gagacctcaa tttggaaaca 11460
atagtagtga aaacaactag actgttgaag gggcatggtt ttacaggtaa tgattctatt 11520
gaacgagttg caggaattaa tatttactct catgaatatt tttgcccaat taactatcgt 11580
acaggtagaa tttcagtcac tgaaaaaact gtatctatac accattacca agaaagttgg 11640
ctttcggctt tagataagat tattaataag atagaacaga gtagaagtgg agttggaact 11700
cttgaataca aagtcagaag aattgtttct aagccattta gaggagttaa tcgtctgaag 11760
aagaagggaa ttttgggatt tctaaaaaaa tgataagact gtggtattct taataggtct 11820
cataagttct tcctaatgat ggtttggttg cttttcatta tagttcttat tggacgtttt 11880
gtctgcattc aaaaagctct ataatctcta cagtagtttt acccactata gaaattatag 11940
agccgaaaaa aatactgtcc aaggctaagg tgcctatcaa atcaaaaatg tccatagaag 12000
aagcgtattc gcatttaaat gcttgtagtc cgagacccaa tgggacttgt tatactatga 12060
acaaaataga aaatgcatat gatttgcaaa ttatcattgc ggcctataat accgaacaat 12120
atataaaaga ttgcttgaat tctgtctgtg gtcaacattc aaaatataaa actttggtga 12180
cagttataaa tgatggttca acagatggaa cagagcatat acttgctggt ataacttcgg 12240
aaaatagggg tggaagcgag ctatgcatca gagttataaa tcaggacaac cgagggcttt 12300
cgggtgccag aaatgctgct ctttctaata ttgaagcaaa gtatgtaatg tttttagact 12360
cagatgacat tctcccagag aatactattt ctgttatgtt agataaagca tttataactg 12420
atgcagatat tttacaagaa agttggtatt cgtttgatga tgaaaatgtg agtgaaaaca 12480
ttctggagga aaaagttttt gaagttggaa caaaaggata tgtttcggga tatccttggg 12540
gtaaactata caaaagttct gtattaaaaa atttttaatt tccaaaagga tactggtttg 12600
aagatacacc aatcactttt attcttgcag caatgccaat taaagtggta accataaagg 12660
atattgttta tggctatcga ctgaatccaa atggaattac agcaaagtca atctttcata 12720
agaaatcagt tgatactttt tgggttacgg aattatgttt gaacgaacta ccaagattca 12780
aagtgtcata tgatcagcaa tcatatgaat atttattaag acaatcattg atgaatgaag 12840
ccaggataaa gaatcaggga aagaaaatac gagaagcagt atttgtcctg acatcagaat 12900
taatgaaaat ttattttagt ggtttttctt caaaagattc aaagatgaag aaaatagaaa 12960
tagcacttag aaatagacaa tttatccagt ttgaattatc aaaactgtat ttgtaatgat 13020
agattaagtg agacagggag aacagttagt gaatagatac agatatttgt taaaaaacat 13080
aggccttttg accctaagta gtttttcaac caaattactt agtttctttt tggtaccgtt 13140
atataccaat atattatcga ctactgaata tggaacctat gatttattta atactacaat 13200
aggtgtatta ctcccgatcc tcacattgaa tatacaggaa gcggtgatga gattttccat 13260
tgacagtaag tatgatagaa aatcaatcgt gacagtgaca gcaaggttct ttattctttc 13320
aaatttaatt gttattcttg ggctttgggt aaattataca tttgtattta gcgtgattgc 13380
caagcagtat gccatatttt tcttcctgat gtttttatcg cagtcgctat ccgggatgat 13440
aaccatgtat gtcagaggaa tagacaaaat atctgacttg tctttttcaa gtgtaatagc 13500
atctgttatt actatatgct taaatgtttc gttcttagca tttcttcact ggggacttgt 13560
tggatatttt atggccaata taatcggccc gatggttcaa tcactttatt tgattgttaa 13620
agcacatatt ttaggggata ttcatttaga gcaagcatat cagtctgaaa aaaatgaaat 13680
ggttaattat agtaaaccat tgattgcaaa cagcatcgcg tggtgggtaa ataatttttc 13740
tgacagatat atcgttgtga ttttttgtgg gttggctgaa aatggaatat attcagtggc 13800
atcgaaaatt ccttcgatac ttaatatttt tcagacaatt tttaatcagg cgtggacgct 13860
gtctgctgta aaggatttcg accctgagga taaaaacggc tttttcacca atacatataa 13920
agcatataat tgcatgatgg ttgttctatg ctctggaatt attgtattta acaaactgtt 13980
agccagtttt ttgtatgcaa aagactttta tgttgcttgg aaatacgtac catggttaac 14040
aattgcaatc gtattcggag caatgtccgg atatattgga ggaatttttg ctgctgtcaa 14100
ggattctaaa atatttgcga aatcaactgt ttgtggagcg ataactaatg taatattaaa 14160
tttaatactt acacccatta tgggaccatt gggagctgct attgctacgg cagtatccta 14220
ttttgaggta tggattttta gatatttgca atccaggaga tatattagga ttagggttaa 14280
tatatttagg gatcttatta cttatttctt gctgttttgt cagtcgatta tacttttgat 14340
tgaaatggaa aattcttatt tatatgttct tgaaatcgga atttttattt tgatagtttt 14400
gctatatttt aaggatattc ttttgctatt gaacaaaggt agtaattcaa tcaaaatgaa 14460
gaaaagggga agtgaattat agtatgaaga tagggataat aacgtggttt acaggctcta 14520
attatggcac taaccttcag gcaatcgctc ttcaatatta cttgagaagt cgtggttatg 14580
ggctctttgt caactgtagt gggtgacgaa aagctaacat ctagagagga ccggataggt 14640
ccttttttta tgtatgttca gtgtgatgaa gacacgcttc ttaaagttga taaagtttct 14700
aaaaccgaag cccaagcgtt tgatgtcttt gatcaactta ttagtcgctt caagtttcgc 14760
gtttgaatag tccgtttcta gtgcgttttt gatgtattgc ttgtgtctaa gaaaagtcct 14820
aaagacagtt tgaaaataat gattgacctt gctcctattt tcctctatca gttcaaagaa 14880
ctcatctact ctcttctcct gaaagtgaaa aaacaaaagc tgataaagtg tatagtagtc 14940
agtaagctct tttgaaaaga ctagtgtctt cgcaacaact tcatgtggtg ctaaagtttg 15000
gcggaaagtc tttgaataaa aagaattgag agatagttta cagctgtcct tttggaagag 15060
tcgccagtga tttttcaagg ctcgataggg tagtgacttc ttatcgaact ggttcatgat 15120
tgcaattctt gtctttaaaa aggcaagtcc aaggtgctgg atgatgtgga aacgatcaag 15180
aacgattttg acgtttggaa agagtctgcg ggctagtggg atataggctc cagacatatc 15240
cattgtgata aactgtacct gttgtcggac tttcaatgga tacttcaaaa agtagtttcg 15300
tatagtagtt tggcggcgat tatcaaggat ggttatgagt tcgtttgtct cataattctg 15360
cgccacaaaa gccaattccc ctttcttgaa cccaaactca tcccaggaca taacagcagg 15420
gagtttgtca taatgttcct tgaaagtaaa ctgatcaagc ttacgataga cagtggacgt 15480
cgacacgcga agtcttcttg caatatcagt tagtgacact ttctcagtta ggagttgtgt 15540
aactttttgt cggactagat tggagatttg gcagtttttc tcaacgatag atgtctcagc 15600
cacccttact ctcctacaac ttttacactg gaaacgacgt tttttcagac gtagtagagt 15660
tggcgttccc gcttgctcga gaagagagat tttagagttt ttttgaaagt catacttgat 15720
catctttcca tggcaatgag gacatgatgg tgaagggtaa tcaagttttg cttgaatctc 15780
gatatgagtg tcagtttcaa aaacaagtga aatcttgata ttttggtctt tgattccgat 15840
taattctgtg ttattcttaa taagtttcat aagttcttcc taatgatggt ttggttgctt 15900
ttcattatag ttcttatggg actttttgtg tacactcaaa aagctctata atccctacag 15960
tagttttacc cactacagaa attatagagc caatatatct cctgtctatt tttatgctac 16020
ttttgggtta gc 16032
<210>2
<211>906
<212>DNA
<213>Streptococcus thermophilus
<400>2
aaggagagat tcgtgaaacc taaaatactt gtacttatgg caacctataa tggaggaaaa 60
tacttacgag agcaattaga tagtgttttt ttacaaaaaa atgtggatat tactgtcctg 120
gttcgtgacg atggttcaat tgacaatacg tgtgctattc ttgatgaata tagtaagaga 180
tacaacttaa tatggtattc agggcaacat ctaaatgttg cgaatggatt ttataatttg 240
atggaagaag cagtcaaaat ggattttgac tacttcgctt tctgtgatca ggatgatgtt 300
tgggatacag ataaactttc catcggagtt tctgcaattt gtagttttaa tgaacctgct 360
ttatattatt gtggtcaaag attggccgac ggtaatttaa attttatagc aaatcatgaa 420
ctcaacacag aacggacact ccatacaagg tttatccttt ctgattttgc tggttgtaca 480
ggagttttca ataaatcgtt attaaaggaa gttgttagct atagaccgaa gtatatgttg 540
atgcatgata catggatatt aaaagtctgt ttggctctag gtggacaggt tattattgat 600
acaagaccgc atatgtatta taggcaacat ggtggtaata cagttggtct tggaagaagt 660
ctatctgcat acttcaaaca ggtaaaacag tatataactg aatataaagt ggaaccacag 720
atgaaggaac ttctcttagg atatggtgat agaattattt cggagtatgc tgtaattgca 780
aatgcttgtt gtaattataa aacggatcgt gaggcacgta cttttttatt aaatagaaat 840
aatgttgatt tttgcacgat gggattaaat atcaccttta aattaaaagt tctattaaat 900
aaatta 906
<210>3
<211>1032
<212>DNA
<213>Streptococcus thermophilus
<400>3
atggaggaag agtttttgct ttttagtatt ataatacctg tttttaatgt cgaaaaatat 60
cttgaggagt gccttctctc gattataggg cagactgatg aaatatctga aggatgtgaa 120
ataattctta ttaatgacgg gtcaacagat gatagtgcga aaatttgtga tgagtatgag 180
cgaaaatatc ctgaattgat aaaggtattt cataggtcaa atcatggttt gcttcttaca 240
cgaaggtttg gatatcaaca tgccgtagga agatacataa ttaattgtga ctcagatgat 300
tcgttggaac cgattgcact tgaggtgctc aaagacgcaa tcgtcgaata caacaaccca 360
gatgttatta tatttaacca caatacatat aaaaatggtg taaaggagat tgcatacaat 420
aacattttta cagagtctgt ttctgcccag atagataagc tagatgtgtt gaaagagttt 480
ctgactggaa atcgtattaa tagcgtttgt ggtgcaattt gtaaaaagga ttgtattgat 540
ataaatagag actataataa atataaaggt cttaataatg gagaagatag ccttcaaaag 600
attgaacaat tcaaaagtgc gaacacgttt gcatatataa ataaacctct ctataattat 660
agggcaggat cgggtatgac cacaaaattt gatcgaaatt attttaaatc ttttagaata 720
gttctggatg aaattcaaaa agaacgagaa atgtgggatt ttcctgatgt agatagatat 780
ttttcaatta aagttttgtc tattgctgga agagcaatca cacagtcaag atacaattgt 840
tgggagaata aaagcgagca aataagatac ttgcatgata ttcgtaatga tgactacttt 900
aaaaaagcag tgcttcaatt ggctacaata aggaaatatc ttcagaaaga ccatatttta 960
ctaataaaga tgttggataa gggtttgatt cgtcttatag ttttgatgtt ggatattaaa 1020
aataagatag gc 1032
<210>4
<211>1140
<212>DNA
<213>Streptococcus thermophilus
<400>4
atgaatagta tagataagaa gtatcgtatt aaacttgata cgttaatatt tttgattttt 60
attatgttag caactttcaa ctattcgatg gagttatcaa tagttggaaa tccattcagg 120
caagtgattc ttgtactgat gctcatatta ttgttgttta caatcatcct taagaaatac 180
acaataaaaa aaatgattgt atttattacg gctatagtgt atggattagt taactatcga 240
gtttcaggtt atacagactt atttatttta ttgcttgcgg cgtatgttgc ggatcaagta 300
gattttaata acgtgttaaa agttctattt tgggaaaaat tagtgatatt tttatcgctt 360
aatatatttt cagtgcttgg tgtaatagag atgactaaat tctccatcaa taagtatcta 420
tccattgtag aagcatatgc tccaggatat gtttcttcga atgtatatgg ctgccaagca 480
ggagttttat ttttacttta tttatcaatt aatcggtata aattgacaaa gttaaaagtt 540
ctattagttt ggtttttgag tgttttagta tacattattt gtagatcaag gacagagcta 600
atcttagtat cagtaacaac agttctttta ttaatatgta ataatccaaa aaactttaat 660
aaagttaaga ggatactctc gtggggatat ccatctattt tggttttaaa ttttgggtta 720
atatatactt tttcaatact cggttacggt aatcccatta tgagcactgt aaatgatgta 780
ttattcaatg ggagaattgg cttggctctc atgaatttta atacttatgg tatttcatta 840
tttggctccc caattgatgt ttcgatagta gctaaaacga atagatatta tgctttagat 900
aatggttata cagttctcgt tttatattac ggcctaatag gattattatg gtattcatat 960
attcaaattc agactgcaaa aaaattagag aagataaatg agttggtcct gatggttgta 1020
ctctttatca tcaatatctg ggggatttat gaaggaaaca tggtttcttt aggtggaaat 1080
ttcatgatta tagcttttct ttctaaagta acagataatt attcagaaga attaaaaagt 1140
<210>5
<211>810
<212>DNA
<213>Streptococcus thermophilus
<400>5
tatatgatac caaaggttat tcactattgt tggtttggag gaaaacctct accagaagat 60
gcaaaaatgt atatcgagtc atggaaaaaa tatttaccga attatgagat aaaagagtgg 120
aatgaaacaa actttgattt gaattgttgc gattatgtta aagaggcata tcaagaaaag 180
aaatgggctt ttgtttcgga ttatgcaaga ctatggatta tttataatta cggtggaatt 240
tattttgata ctgatgtcga gctaataagt tctcctgaac caatcataga aaatggagct 300
tttttaggat gtgaactcaa tttcgaaaag gaattatcag gtgatactaa ccaaactgaa 360
cttgttaacc caggtctagg ttttggtgcg gaaccgaaga aggattttta tcgtgagcta 420
ttaaatttat acgaatcaca acactttatt gataaaaatg gagacctcaa tttggaaaca 480
atagtagtga aaacaactag actgttgaag gggcatggtt ttacaggtaa tgattctatt 540
gaacgagttg caggaattaa tatttactct catgaatatt tttgcccaat taactatcgt 600
acaggtagaa tttcagtcac tgaaaaaact gtatctatac accattacca agaaagttgg 660
ctttcggctt tagataagat tattaataag atagaacaga gtagaagtgg agttggaact 720
cttgaataca aagtcagaag aattgtttct aagccattta gaggagttaa tcgtctgaag 780
aagaagggaa ttttgggatt tctaaaaaaa 810
<210>6
<211>750
<212>DNA
<213>Streptococcus thermophilus
<400>6
gttcttccta atgatggttt ggttgctttt cattatagtt cttattggac gttttgtctg 60
cattcaaaaa gctctataat ctctacagta gttttaccca ctatagaaat tatagagccg 120
aaaaaaatac tgtccaaggc taaggtgcct atcaaatcaa aaatgtccat agaagaagcg 180
tattcgcatt taaatgcttg tagtccgaga cccaatggga cttgttatac tatgaacaaa 240
atagaaaatg catatgattt gcaaattatc attgcggcct ataataccga acaatatata 300
aaagattgct tgaattctgt ctgtggtcaa cattcaaaat ataaaacttt ggtgacagtt 360
ataaatgatg gttcaacaga tggaacagag catatacttg ctggtataac ttcggaaaat 420
aggggtggaa gcgagctatg catcagagtt ataaatcagg acaaccgagg gctttcgggt 480
gccagaaatg ctgctctttc taatattgaa gcaaagtatg taatgttttt agactcagat 540
gacattctcc cagagaatac tatttctgtt atgttagata aagcatttat aactgatgca 600
gatattttac aagaaagttg gtattcgttt gatgatgaaa atgtgagtga aaacattctg 660
gaggaaaaag tttttgaagt tggaacaaaa ggatatgttt cgggatatcc ttggggtaaa 720
ctatacaaaa gttctgtatt aaaaaatttt 750
<210>7
<211>435
<212>DNA
<213>Streptococcus thermophilus
<400>7
tttccaaaag gatactggtt tgaagataca ccaatcactt ttattcttgc agcaatgcca 60
attaaagtgg taaccataaa ggatattgtt tatggctatc gactgaatcc aaatggaatt 120
acagcaaagt caatctttca taagaaatca gttgatactt tttgggttac ggaattatgt 180
ttgaacgaac taccaagatt caaagtgtca tatgatcagc aatcatatga atatttatta 240
agacaatcat tgatgaatga agccaggata aagaatcagg gaaagaaaat acgagaagca 300
gtatttgtcc tgacatcaga attaatgaaa atttatttta gtggtttttc ttcaaaagat 360
tcaaagatga agaaaataga aatagcactt agaaatagac aatttatcca gtttgaatta 420
tcaaaactgt atttg 435
<210>8
<211>1452
<212>DNA
<213>Streptococcus thermophilus
<400>8
gtgagacagg gagaacagtt agtgaataga tacagatatt tgttaaaaaa cataggcctt 60
ttgaccctaa gtagtttttc aaccaaatta cttagtttct ttttggtacc gttatatacc 120
aatatattat cgactactga atatggaacc tatgatttat ttaatactac aataggtgta 180
ttactcccga tcctcacatt gaatatacag gaagcggtga tgagattttc cattgacagt 240
aagtatgata gaaaatcaat cgtgacagtg acagcaaggt tctttattct ttcaaattta 300
attgttattc ttgggctttg ggtaaattat acatttgtat ttagcgtgat tgccaagcag 360
tatgccatat ttttcttcct gatgttttta tcgcagtcgc tatccgggat gataaccatg 420
tatgtcagag gaatagacaa aatatctgac ttgtcttttt caagtgtaat agcatctgtt 480
attactatat gcttaaatgt ttcgttctta gcatttcttc actggggact tgttggatat 540
tttatggcca atataatcgg cccgatggtt caatcacttt atttgattgt taaagcacat 600
attttagggg atattcattt agagcaagca tatcagtctg aaaaaaatga aatggttaat 660
tatagtaaac cattgattgc aaacagcatc gcgtggtggg taaataattt ttctgacaga 720
tatatcgttg tgattttttg tgggttggct gaaaatggaa tatattcagt ggcatcgaaa 780
attccttcga tacttaatat ttttcagaca atttttaatc aggcgtggac gctgtctgct 840
gtaaaggatt tcgaccctga ggataaaaac ggctttttca ccaatacata taaagcatat 900
aattgcatga tggttgttct atgctctgga attattgtat ttaacaaact gttagccagt 960
tttttgtatg caaaagactt ttatgttgct tggaaatacg taccatggtt aacaattgca 1020
atcgtattcg gagcaatgtc cggatatatt ggaggaattt ttgctgctgt caaggattct 1080
aaaatatttg cgaaatcaac tgtttgtgga gcgataacta atgtaatatt aaatttaata 1140
cttacaccca ttatgggacc attgggagct gctattgcta cggcagtatc ctattttgag 1200
gtatggattt ttagatattt gcaatccagg agatatatta ggattagggt taatatattt 1260
agggatctta ttacttattt cttgctgttt tgtcagtcga ttatactttt gattgaaatg 1320
gaaaattctt atttatatgt tcttgaaatc ggaattttta ttttgatagt tttgctatat 1380
tttaaggata ttcttttgct attgaacaaa ggtagtaatt caatcaaaat gaagaaaagg 1440
ggaagtgaat ta 1452
Claims (17)
1.包含选自由核苷酸序列SEQ ID No.1、SEQ ID No.2、SEQ ID No.3、SEQ ID No.4、SEQ ID No.5、SEQ ID No.6、SEQ ID No.7、SEQ ID No.8组成的组的至少一个序列的乳酸细菌菌株；或包含核酸的乳酸细菌菌株，该核酸包含至少一个ORF，该ORF的翻译产物具有相同残基大于或等于80％的百分比且具有序列SEQ ID No.1至SEQ ID No.8中鉴定的ORFs所得到的至少一个多肽序列。
2.根据权利要求1的乳酸细菌菌株，其特征在于它选自链球菌属(Streptococcus)、乳球菌属(Lactococcus)、乳杆菌属(Lactobacillus)、明串珠菌属(Leuconostoc)、小球菌属(Pediococcus)和双歧杆菌属(Bifidobacterium)。
3.根据权利要求1或2的乳酸细菌菌株，其特征在于它属于嗜热链球菌种。
4.2003年2月26日保藏在国立微生物保藏中心，保藏号为I-2980的嗜热链球菌菌株。
5.由包含选自核苷酸序列SEQ ID No.1、SEQ ID No.2、SEQ ID No.3、SEQ ID No.4、SEQ ID No.5、SEQ ID No.6、SEQ ID No.7、SEQ ID No.8组成的组的至少一个序列或包含选自由核苷酸序列SEQ ID No.1、SEQ IDNo.2、SEQ ID No.3、SEQ ID No.4、SEQ ID No.5、SEQ ID No.6、SEQ IDNo.7、SEQ ID No.8组成的组的至少一个序列的核酸；或包含至少一个ORF，该ORF的翻译产物具有相同残基大于或等于80％的百分比且具有序列SEQ ID No.1至SEQ ID No.8中鉴定的ORFs所得到的至少一个多肽序列的核酸的克隆和/或表达载体或质粒转化的宿主细菌。
6.根据权利要求5的细菌，其特征在于它是乳酸细菌。
7.根据权利要求5或6的细菌，其特征在于它是选自链球菌属(Streptococcus)、乳球菌属(Lactococcus)、乳杆菌属(Lactobacillus)、明串珠菌属(Leuconostoc)、小球菌属(Pediococcus)和双歧杆菌属(Bifidobacterium)的乳酸细菌。
8.构建根据权利要求1至4的菌株或根据权利要求5至7的细菌的方法，其特征在于该菌株或细菌是通过使用包含选自由核苷酸序列SEQ IDNo.1、SEQ ID No.2、SEQ ID No.3、SEQ ID No.4、SEQ ID No.5、SEQ IDNo.6、SEQ ID No.7、SEQ ID No.8组成的组的至少一个序列或包含选自由核苷酸序列SEQ ID No.1、SEQ ID No.2、SEQ ID No.3、SEQ ID No.4、SEQ ID No.5、SEQ ID No.6、SEQ ID No.7、SEQ ID No.8组成的组的至少一个序列的核酸，或包含至少一个ORF，该ORF的翻译产物具有相同残基大于或等于80％的百分比且具有序列SEQ ID No.1至SEQ ID No.8中鉴定的ORFs所得到的至少一个多肽序列的核酸的载体转化获得的。
9.根据权利要求8的方法，其特征在于转化之后是通过至少一个重组结果插入到菌株或宿主细菌的基因组。
10.根据权利要求8或9的方法，其特征在于该载体是质粒。
11.包含根据权利要求1或4的至少一个菌株或根据权利要求8至10的方法获得的至少一个菌株或根据权利要求5至7的至少一个转化细菌的细菌组合物。
12.根据权利要求1至4的菌株或根据权利要求8至10的方法获得的菌株或根据权利要求11的细菌组合物为生产食品或食品成分的用途。
13.根据权利要求12的用途，其特征在于食品或食品成分是乳制品、肉制品、谷类制品、饮料、起泡剂或粉剂。
14.包含根据权利要求1至4的至少一个菌株或根据权利要求8至10的方法获得的至少一个菌株或根据权利要求11的细菌组合物的食品或药物组合物。
15.包含根据权利要求1至4的至少一个菌株或根据权利要求8至10的方法获得的至少一个菌株或根据权利要求11的细菌组合物的乳制品。
16.根据权利要求15的乳制品，其特征在于它是发酵乳、酸奶、成熟奶油、乳酪、清爽干酪、乳制饮料、乳制品截留物、融化干酪、奶油甜点、酪农干酪或婴儿奶。
17.根据权利要求15或16的乳制品，其特征在于它包含动物和/或植物来源的奶。
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FR0303242A FR2852604B1 (fr) | 2003-03-17 | 2003-03-17 | Bacteries lactiques texturantes |
| FR0303242 | 2003-03-17 | ||
| CNA2004800072432A CN1761752A (zh) | 2003-03-17 | 2004-03-12 | 组织形成乳酸细菌 |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNA2004800072432A Division CN1761752A (zh) | 2003-03-17 | 2004-03-12 | 组织形成乳酸细菌 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102409004A true CN102409004A (zh) | 2012-04-11 |
| CN102409004B CN102409004B (zh) | 2016-09-07 |
Family
ID=32922222
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNA2004800072432A Pending CN1761752A (zh) | 2003-03-17 | 2004-03-12 | 组织形成乳酸细菌 |
| CN200910224873.XA Expired - Lifetime CN102409004B (zh) | 2003-03-17 | 2004-03-12 | 组织形成乳酸细菌 |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNA2004800072432A Pending CN1761752A (zh) | 2003-03-17 | 2004-03-12 | 组织形成乳酸细菌 |
Country Status (13)
| Country | Link |
|---|---|
| US (1) | US7582743B2 (zh) |
| EP (1) | EP1604025B2 (zh) |
| JP (1) | JP4669835B2 (zh) |
| CN (2) | CN1761752A (zh) |
| AR (1) | AR043607A1 (zh) |
| AU (1) | AU2004223691B2 (zh) |
| DE (1) | DE602004022028D1 (zh) |
| DK (1) | DK1604025T4 (zh) |
| ES (1) | ES2329470T5 (zh) |
| FR (1) | FR2852604B1 (zh) |
| NZ (1) | NZ542597A (zh) |
| RU (1) | RU2376367C2 (zh) |
| WO (1) | WO2004085607A2 (zh) |
Families Citing this family (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| PL1991660T3 (pl) * | 2006-02-24 | 2011-10-31 | Chr Hansen As | Bakterie kwasu mlekowego dostarczające poprawionej tekstury fermentowanym produktom mlecznym |
| AU2007258872A1 (en) | 2006-06-16 | 2007-12-21 | Danisco A/S | Bacterium |
| EP1869983A1 (en) * | 2006-06-23 | 2007-12-26 | Chr. Hansen A/S | Low post-acidifying lactic acid bacteria |
| FR2906536B1 (fr) * | 2006-10-03 | 2008-12-26 | Danisco | Cluster genetique de souches de streptococcus thermophilus presentant des proprietes acidifiantes et texturantes appropriees pour les fermentations laitieres. |
| EP2789687A1 (en) | 2008-12-12 | 2014-10-15 | DuPont Nutrition Biosciences ApS | Genetic cluster of strains of Streptococcus thermophilus having unique rheological properties for dairy fermentation |
| MX2011013188A (es) * | 2009-06-30 | 2012-01-31 | Chr Hansen As | Metodo para producir un producto lacteo fermentado. |
| WO2011004012A2 (en) | 2009-07-10 | 2011-01-13 | Chr. Hansen A/S | Production of cottage cheese by using streptococcus thermophilus |
| KR101773994B1 (ko) * | 2009-09-01 | 2017-09-01 | 시에이치알. 한센 에이/에스 | 엑소다당류의 과발현에 의하여 식품을 조직화하기 위하여 갈락토키나아제 발현을 변형시킨 젖산균 |
| TR201807378T4 (tr) * | 2010-01-28 | 2018-06-21 | Chr Hansen As | Faj dayanımına göre seçilen gıda ürünlerinin tekstüre edilmesi için laktik bakteriler. |
| KR101579556B1 (ko) * | 2010-06-21 | 2015-12-22 | 시에이치알. 한센 에이/에스 | 요거트용 젖산 박테리아 |
| MX346975B (es) | 2010-10-22 | 2017-04-07 | Chr Hansen As | Cepas texturizadoras de bacterias de acido lactico. |
| RU2455363C1 (ru) * | 2011-03-23 | 2012-07-10 | Государственное научное учреждение Институт экспериментальной ветеринарии Сибири и Дальнего Востока Российской академии сельскохозяйственных наук (ГНУ ИЭВСиДВ Россельхозакадемии) | Синтетические олигонуклеотидные праймеры и способ выявления streptococcus thermophilus в заквасочных культурах при производстве твердых сыров |
| US20140134292A1 (en) | 2011-04-20 | 2014-05-15 | Dupont Nutrition Biosciences Aps | Production of cheese with s. thermophilus |
| EP3116994B1 (en) * | 2014-02-20 | 2019-07-24 | DSM IP Assets B.V. | Phage-insensitive streptococcus thermophilus |
| EP3133929A1 (en) * | 2014-04-23 | 2017-03-01 | DSM IP Assets B.V. | Fermented milk product |
| WO2016164096A1 (en) | 2015-04-06 | 2016-10-13 | Dupont Nutrition Biosciences Aps | Proteases for high protein fermented milk products |
| KR20190044664A (ko) | 2016-09-01 | 2019-04-30 | 시에이치알. 한센 에이/에스 | 새로운 세균 |
| CN109735556A (zh) * | 2019-02-22 | 2019-05-10 | 昆明理工大学 | 引导糖基转移酶基因的用途 |
| JPWO2022080246A1 (zh) * | 2020-10-12 | 2022-04-21 | ||
| WO2022080245A1 (ja) * | 2020-10-12 | 2022-04-21 | 株式会社明治 | 乳酸菌の菌体外多糖の免疫賦活活性の向上作用を有するタンパク質、並びに、それを用いた発酵乳及びその製造方法 |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2001079500A2 (fr) * | 2000-04-18 | 2001-10-25 | Institut National De La Recherche Agronomique (Inra) | Operons de streptococcus thermophilus impliques dans la synthese des exopolysaccharides (eps) |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2627779B1 (fr) † | 1988-02-26 | 1991-03-15 | Sodima Union Coop Agricoles | Procede d'epaississement d'un produit du type lait fermente, notamment de yaourt et nouveau polysaccharide utile pour la mise en oeuvre de ce procede |
| EP0750042A1 (fr) * | 1995-06-20 | 1996-12-27 | Societe Des Produits Nestle S.A. | Bactéries lactiques produisant des exopolysaccharides |
| WO2001057234A2 (en) * | 2000-02-02 | 2001-08-09 | The State Of Oregon Acting By And Through The State Board Of Higher Education On Behalf Of Oregon State University | Biopolymer thickener |
| HU224134B1 (hu) † | 2000-07-24 | 2005-05-30 | Magyar Tejgazdasági Kísérleti Intézet Kft. | Probiotikus hatású, a vér koleszterinszintjét csökkentő kefir és eljárás előállítására |
-
2003
- 2003-03-17 FR FR0303242A patent/FR2852604B1/fr not_active Expired - Lifetime
-
2004
- 2004-03-12 DE DE602004022028T patent/DE602004022028D1/de not_active Expired - Lifetime
- 2004-03-12 DK DK04720033.2T patent/DK1604025T4/en active
- 2004-03-12 US US10/548,940 patent/US7582743B2/en active Active
- 2004-03-12 ES ES04720033T patent/ES2329470T5/es not_active Expired - Lifetime
- 2004-03-12 WO PCT/FR2004/000610 patent/WO2004085607A2/fr active Application Filing
- 2004-03-12 NZ NZ542597A patent/NZ542597A/en not_active IP Right Cessation
- 2004-03-12 RU RU2005131964/13A patent/RU2376367C2/ru active
- 2004-03-12 CN CNA2004800072432A patent/CN1761752A/zh active Pending
- 2004-03-12 EP EP04720033.2A patent/EP1604025B2/fr not_active Expired - Lifetime
- 2004-03-12 CN CN200910224873.XA patent/CN102409004B/zh not_active Expired - Lifetime
- 2004-03-12 JP JP2006505724A patent/JP4669835B2/ja not_active Expired - Lifetime
- 2004-03-12 AU AU2004223691A patent/AU2004223691B2/en not_active Expired
- 2004-03-15 AR ARP040100848A patent/AR043607A1/es active IP Right Grant
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2001079500A2 (fr) * | 2000-04-18 | 2001-10-25 | Institut National De La Recherche Agronomique (Inra) | Operons de streptococcus thermophilus impliques dans la synthese des exopolysaccharides (eps) |
Non-Patent Citations (2)
| Title |
|---|
| BROADBENT JR等: "Biochemistry, genetics, and applications of exopolysaccharide production in Streptococcus thermophilus: a review", 《J DAIRY SCI.》 * |
| PLUVINET,A.等: "Streptococcus thermophilus eps locus", 《GENBANK: AJ289861.1》 * |
Also Published As
| Publication number | Publication date |
|---|---|
| RU2005131964A (ru) | 2006-03-10 |
| EP1604025A2 (fr) | 2005-12-14 |
| CN1761752A (zh) | 2006-04-19 |
| EP1604025B1 (fr) | 2009-07-15 |
| AU2004223691A1 (en) | 2004-10-07 |
| RU2376367C2 (ru) | 2009-12-20 |
| FR2852604A1 (fr) | 2004-09-24 |
| AR043607A1 (es) | 2005-08-03 |
| DE602004022028D1 (de) | 2009-08-27 |
| US20060240539A1 (en) | 2006-10-26 |
| WO2004085607A2 (fr) | 2004-10-07 |
| WO2004085607A3 (fr) | 2005-03-17 |
| AU2004223691A2 (en) | 2004-10-07 |
| EP1604025B2 (fr) | 2018-10-03 |
| DK1604025T3 (da) | 2009-11-02 |
| NZ542597A (en) | 2008-03-28 |
| ES2329470T3 (es) | 2009-11-26 |
| AU2004223691B2 (en) | 2008-04-03 |
| JP2006520590A (ja) | 2006-09-14 |
| US7582743B2 (en) | 2009-09-01 |
| DK1604025T4 (en) | 2019-01-28 |
| ES2329470T5 (es) | 2019-04-16 |
| CN102409004B (zh) | 2016-09-07 |
| FR2852604B1 (fr) | 2005-05-13 |
| JP4669835B2 (ja) | 2011-04-13 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102409004B (zh) | 组织形成乳酸细菌 | |
| EP2034848B1 (en) | Streptococcus thermophilus bacterium | |
| CN102695420B (zh) | 用于通过过量表达胞外多糖质构化食品的具有修饰的半乳糖激酶表达的乳酸细菌 | |
| CN103025863B (zh) | 用于酸奶的乳酸细菌 | |
| TW200823288A (en) | Genetic cluster of strains of streptococcus thermophilus having appropriate acidifying and texturizing properties for dairy fermentations | |
| US8741622B2 (en) | Stress tolerant Bifidobacteria | |
| CN109312292A (zh) | 乳酸菌发酵促进剂 | |
| US6258587B1 (en) | Recombinant, sequence-modified L. johnsonii bacteria which produce only L(+)-lactate | |
| US20030186392A1 (en) | Biopolymer thickener | |
| CN114616319A (zh) | 含有nizo b40样eps基因簇的质地化乳酸乳球菌 | |
| JP2000102380A (ja) | 乳酸菌生育促進剤及びその利用 | |
| WO2024062838A1 (ja) | 発酵組成物及び発酵組成物の製造方法 | |
| Knoshaug | Exopolysaccharide biosynthesis by a natural lactococcal ropy isolate | |
| Chaiña Salas | Identification and Characterization of Exopolysaccharide Biosynthesis Gene Cluster in Lactobacillus fermentum TDS030603 | |
| AU2001239737A1 (en) | Biopolymer thickener | |
| MXPA98003427A (en) | Production of l (+) - lact | |
| UA113270C2 (xx) | Молочнокисла бактерія streptococcus thermophilus з модифікованою галактокіназною експресією для текстурування харчових продуктів за рахунок надекспресії екзополісахариду |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C53 | Correction of patent of invention or patent application | ||
| CB02 | Change of applicant information |
Address after: Copenhagen, Denmark Applicant after: DUPONT NUTRITION BIOSCIENCES APS Address before: Copenhagen, Denmark Applicant before: DANISCO A/S |
|
| COR | Change of bibliographic data |
Free format text: CORRECT: APPLICANT; FROM: DANISCO TO: DUPONT NUTRITION BIOSCIENCES CO., LTD. |
|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CX01 | Expiry of patent term |
Granted publication date: 20160907 |
|
| CX01 | Expiry of patent term |