CN102406556A - Cosmetic composition favorable for keeping biological activity of epidermal growth factor (EGF) - Google Patents

Cosmetic composition favorable for keeping biological activity of epidermal growth factor (EGF) Download PDF

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CN102406556A
CN102406556A CN2011103621240A CN201110362124A CN102406556A CN 102406556 A CN102406556 A CN 102406556A CN 2011103621240 A CN2011103621240 A CN 2011103621240A CN 201110362124 A CN201110362124 A CN 201110362124A CN 102406556 A CN102406556 A CN 102406556A
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growth factor
epidermal growth
biological activity
cosmetic composition
egf
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CN2011103621240A
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Chinese (zh)
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刘光荣
李建华
马忠华
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无限极(中国)有限公司
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Abstract

The invention discloses a cosmetic composition favorable for keeping the biological activity of an epidermal growth factor (EGF). The composition mainly comprises the EGF, hydrolyzed collagen powder, sodium hyaluronate, silk peptide powder, palmitoyl pentapeptide and carnosine. The cosmetic composition disclosed by the invention has the characteristics that polypeptides with different structures and molecules are used as main raw materials, polysaccharides are compounded, and various components are similar to the EGF in structure, thereby protecting the EGF; and under the protective action of multiple components which are reasonably combined and matched, the biological activity of the EGF in a cosmetic formula is kept.

Description

一种有助于保持表皮生长因子生物活性的化妆品组合物 A method that facilitates retaining the biological activity of epidermal growth factor cosmetic composition

技术领域 FIELD

[0001] 本发明涉及化妆品领域,具体涉及一种有助于保持表皮生长因子生物活性的组合物。 [0001] The present invention relates to the field of cosmetics, particularly, to a composition helps to maintain the biological activity of the epidermal growth factor.

背景技术 Background technique

[0002] 目前市场上含表皮生长因子(EGF)的护肤品,通过表皮生长因子(EGF)刺激表皮细胞新陈代谢的作用,加速皮肤新生细胞替代衰老细胞的进程,促使皮肤细胞生长,保持皮肤细胞年轻化,从而达到保持皮肤年轻状态、焕发青春的目的。 [0002] currently contains epidermal growth factor (EGF) on the market skin care products, stimulate skin cell metabolism by the action of epidermal growth factor (EGF), to accelerate new skin cell replacement cell aging process, promote the growth of skin cells and keep skin cells young and technology, so as to achieve to keep the skin young state, rejuvenated purposes. 因为它的效果比一般护肤品成份好,且天然无副作用,因此表皮生长因子(EGF)已成为高档美容护肤产品的有效成份之一,并在市场上广受欢迎。 Because it works better than the average skin care ingredients, natural and without side effects, so the epidermal growth factor (EGF) has become one of the active ingredients of high-end skin care products, and popular in the market.

[0003] 但是表皮生长因子作为一种具生物活性的多肽分子,在实际的化妆品配方中的生物活性比较难保持,因为化妆品配方中的成分比较复杂且不同的配方成分对其生物活性有较大的影响,有的可能降解而失去生物活性。 [0003] However, epidermal growth factor polypeptide as a molecule having biological activity, the biological activity of the actual cosmetic formulations more difficult to maintain because of cosmetic formulations and distinct component complex formulation ingredients its greater biological activity effects, and some may degradation and loss of biological activity. 只有确保添加到护肤品中的表皮生长因子(EGF)维持生物活性,才能保证产品的功效。 Only by ensuring added to skin care products in the epidermal growth factor (EGF) to maintain biological activity, in order to ensure the effectiveness of the product. 因此如何在化妆品配方中能更好的保持表皮生长因子(EGF)的生物活性,是化妆品领域中一个普遍关心且具有重要意义的问题。 So how can better maintain the biological activity of the epidermal growth factor (EGF) in cosmetic formulations, it is a common concern in the field of cosmetics and significance of the problem.

发明内容 SUMMARY

[0004] 本发明的目的是提供一种有助于保持表皮生长因子(EGF)生物活性的化妆品组合物。 [0004] The object of the present invention is to provide a help to maintain epidermal growth factor (EGF) the biological activity of cosmetic compositions.

[0005] 本发明提供的一种有助于保持表皮生长因子生物活性的化妆品组合物的主要成分是表皮生长因子(EGF)、水解胶原蛋白粉、透明质酸钠、丝肽粉、棕榈酰五肽和肌肽,辅助成分为化妆品外用剂型的常用基质以及去离子水或纯化水或蒸馏水。 The main component [0005] A method of the present invention to provide aid in maintaining the biological activity of the epidermal growth factor cosmetic composition epidermal growth factor (EGF), hydrolyzed collagen powder, sodium hyaluronate, silk peptide powders, Palmitoyl Pentapeptide and peptide carnosine, cosmetic adjunct ingredients commonly used in topical form of a matrix and deionized water or purified water or distilled water.

[0006] 制备本发明化妆品组合物的组分的百分数含量为: [0006] Preparation of the percentage content of component cosmetic composition of the present invention are:

表皮生长因子水解胶原蛋白粉透明质酸钠丝肽粉棕榈酰五肽肌肽 EGF hydrolyzed collagen peptide powder silk powder of sodium hyaluronate Palmitoyl Pentapeptide carnosine

0.0000001 〜0. 000003 0. 01 〜4. 00 3. 00 5. 00 8. 00 -3. 00 0.0000001 ~ 0. 000003 ~ 4 0.01 00 3.00 5.00 8.00 -3. 00

0. 01 -0. 01 -0. 50 -0. 01 0. 01-0. 01-0. 50-0. 01

余量为化妆品外用剂型的常用基质以及去离子水或纯化水或蒸馏水。 The balance being conventional cosmetic topical dosage form matrix and deionized water or purified water or distilled water. [0007] 制备本发明化妆品组合物的组分的优选百分数含量为: [0007] Preferably the percentage content of the component of the preparation of cosmetic compositions of the present invention are:

0.000003 0.000003

表皮生长因子 0. 000001 I水解胶原蛋白粉 0. 20 - -3. 00透明质酸钠 0. 10 - -2. 00丝肽粉 0. 10 - -3. 00棕榈酰五肽 1. 00 - -5. 00肌肽 0. 20〜2. 00 Epidermal growth factor 0. 000001 I collagen hydrolyzate powder 0.20 - sodium hyaluronate 0.10 -300 -. -200 silk peptide powder 0.10 - -300 Palmitoyl Pentapeptide A peptide 00 - -5. 00 carnosine 0.5 20~2. 00

余量为化妆品外用剂型的常用基质以及去离子水或纯化水或蒸馏水。 The balance being conventional cosmetic topical dosage form matrix and deionized water or purified water or distilled water. [0008] 制备本发明化妆品组合物的组分的最佳百分数含量为: [0008] The optimum percentage content of the component for preparing the cosmetic composition according to the present invention are:

表皮生长因子 0.0000015水解胶原蛋白粉 1. 50透明质酸钠 0. 20丝肽粉 0. 80棕榈酰五肽 2. 00肌肽 0. 40余量为化妆品外用剂型的常用基质以及去离子水或纯化水或蒸馏水。 EGF 0.0000015 hydrolyzed collagen powder of sodium hyaluronate 0.20 1.50 0.80 Silk peptide powder Palmitoyl Pentapeptide Carnosine 0.40 2.00 balance common cosmetic topical dosage form or matrix purified and deionized water water or distilled water.

[0009] 本发明化妆品组合物可以采用常规工艺制成含膏、霜、乳液、化妆水或凝胶等形式。 [0009] The cosmetic composition of the present invention may be made by conventional processes including cream, cream, emulsion, lotion, or gel form and the like.

[0010] 制备本发明化妆品组合物的原料中,水解胶原蛋白粉是以动物胶原蛋白,深海鱼皮为原料经过净华提纯、酶解、过滤、浓缩、喷雾干燥等处理而制成的粉末状产品,其颜色一般为白色或蛋黄色,并且主要由分子量在500〜20000道尔顿之间的多肽组成。 [0010] The cosmetic composition of the starting material was prepared according to the present invention, the hydrolysis of animal collagen is a collagen powder, as raw material after deep skin net Chinese purified enzyme, filtered, concentrated, and spray drying to prepare a powdered processing product, which color is generally white or yellow eggs, and is mainly composed of a molecular weight between 500~20000 Daltons polypeptide. 一般分子量在1000〜5000道尔顿,发挥最佳作用的平均分子量应在700〜1000道尔顿范围。 Generally 1000~5000 daltons molecular weight, works best when the average molecular weight should be in the range of 700~1000 Daltons.

[0011] 透明质酸钠是透明质酸的钠盐形式,是由葡糖醛酸-N-乙酰氨基葡糖为双糖单位组成的直连高分子多糖。 [0011] sodium hyaluronate are hyaluronic acid sodium salt, glucuronic acid is -N- acetylglucosamine disaccharide units of the polysaccharide polymer are directly connected. 透明质酸是天然存在的黏多糖中唯一没有硫酸化的多糖,亲水性强,是无支链的直链线性分子,是皮肤天然存在的成分物质,与组织细胞有高度亲和力。 Hyaluronic acid is a naturally occurring mucopolysaccharide not only sulfated polysaccharides, hydrophilicity, unbranched straight chain linear molecules, is a naturally occurring component of the skin material, a high affinity with tissue cells. 由微生物发酵法生产制得,分子量控制在小于50万道尔顿。 Produced by the microbial fermentation obtained, a molecular weight of less than 500,000 daltons in the control.

[0012] 丝肽粉是将蚕丝脱胶后的丝素经溶解、透析、提纯、降解、浓缩,喷雾干燥等处理而制成的分子量较小的丝蛋白制品。 [0012] Silk peptide powder is after degummed silk fibroin was dissolved, dialyzed, purification, degradation, concentration, and spray drying process and made of silk protein products of smaller molecular weight. 富含十八种氨基酸,其分子量一般在2000以下,这种分子量的丝肽水溶性良好,并能保持丝素结晶的优良特性。 Rich in eighteen amino acids, generally having a molecular weight below 2000, the molecular weight of such water-soluble fine silk peptide, and can keep excellent characteristics crystalline silk fibroin. 丝肽用于化妆品中具有天然保湿、 调湿和营养肌肤的作用,能有效抑制皮肤黑色素的生成,其良好的吸收性和吸附性,使其具有极为优异的护发、养发功能。 Silk peptide having a natural moisturizer used in cosmetics, skin nutrients and the humidity, can effectively inhibit the formation of melanin in the skin, its good absorption and adsorption, it has extremely excellent hair conditioning, hair care function.

[0013] 棕榈酰五肽是是指由赖氨酸-苏氨酸-苏氨酸-赖氨酸-丝氨酸(Lys-Thr-Thr-Lys-Ser)(缩写为KTTKS)这五种氨基酸按顺序组成的五肽的衍生物即棕榈酰-Lys-Thr-Thr-Lys-Ser (缩写为Pal-KTTKS),KTTKS是胶原蛋白前体I最短的片断,在胶原蛋白溶解的过程中被释放出来,可以刺激合成胶原蛋白I、III和人体成纤维细胞中的纤连蛋白,原始的KTTKS多肽经棕榈酰化处理,以提高生物利用度,增强其穿透角质层的能力。 [0013] Palmitoyl Pentapeptide yes refers lysine - Thr - Thr - Lys - Ser (Lys-Thr-Thr-Lys-Ser) (abbreviated as KTTKS) five amino acid sequence pentapeptide derivative composition, i.e. palmitoyl -Lys-Thr-Thr-Lys-Ser (abbreviated Pal-KTTKS), KTTKS body is shortest fragment I procollagen, collagen during the dissolved is released, can stimulate the synthesis of collagen I, III, and fibroblasts of human fibronectin, KTTKS original polypeptide by palmitoylated processed to enhance bioavailability, enhance its ability to penetrate the stratum corneum.

[0014] 肌肽(丙氨酰组氨酸,Carnosine)是由β -丙氨酸和组氨酸组成的二肽,肌肽具有很强的抗氧化能力,它能够稳定地保护细胞膜,特别是作为一种水溶性的自由基清除剂,它能阻止细胞膜的过延氧化反应。 [0014] Carnosine (alanyl-histidine, Carnosine) by β - alanine dipeptide consisting of histidine and carnosine has strong antioxidant capacity, it is possible to stably protect cell membranes, particularly as a water-soluble free-radical scavengers, it can prevent the extension of the oxidation reaction over the cell membrane. 与以往其它的生物类抗氧化剂如VE、V。 Other conventional biological antioxidants such as VE, V. 相比,肌肽的抗氧化能力更强,从而可以延缓因自由基引起的肌肤衰老。 Compared the antioxidant capacity of stronger carnosine, which can delay due to skin aging caused by free radicals. 此外,肌肽还有助于肌肤组织的修复, 对因紫外引起的皮肤过敏具有治疗和预防作用。 In addition, carnosine also helps repair skin tissue with treatment and prevention of skin allergies due to UV-induced.

[0015] 本发明化妆品组合物的特点是以不同结构和分子的多肽为主要原料,复配多糖, 利用各种成分结构和EGF相似从而对其进行保护,合理组合、配合共用,通过多组份的保护作用来达到在化妆品配方中保持EGF活性的作用。 [0015] The cosmetic composition of the features of the present invention is based on different structures and polypeptide molecules as the main raw material, complex polysaccharides, with various components and structures similar to EGF so as to protect it, the rational combination with a common, multi-component by the protective effect of EGF to achieve to keep active role in cosmetic formulations. [0016] 下面通过具体实施方式及实验研究结果对本发明作进一步说明。 [0016] The following experimental results and by way of specific embodiments of the present invention will be further described. 附图说明 BRIEF DESCRIPTION

[0017] 图1为本发明实施例4的产品与空白对照组的EGF生物活性随时间变化趋势统计图。 [0017] FIG. 1 EGF biological activity is related to the control group of Example 4 the trend versus time chart of the present invention.

具体实施方式 Detailed ways

[0018] 本发明提供的一种有助于保持表皮生长因子(EGF)生物活性的化妆品组合物以表皮生长因子(EGF)、水解胶原蛋白粉、透明质酸钠、丝肽粉、棕榈酰五肽、肌肽为主要成分, 并添加化妆品常用基质成分乳化剂、油脂、润肤剂、保湿剂、胶凝剂、甘油、丙二醇、防腐剂、 香精以及去离子水或纯化水或蒸馏水制成。 [0018] A present invention is provided to help to maintain epidermal growth factor (EGF) The biological activity of the cosmetic composition of epidermal growth factor (EGF), hydrolyzed collagen powder, sodium hyaluronate, silk peptide powders, Palmitoyl Pentapeptide peptide, carnosine as a main component, conventional matrix components and add cosmetic emulsifiers, oils, emollients, humectants, gelling agents, glycerol, propylene glycol, a preservative, a perfume and deionized water or purified water or distilled water is made. 下面列举一部分具体实施例对本发明进行说明,有必要在此指出的是以下具体实施例只用于对本发明作进一步说明,不代表对本发明保护范围的限制。 The following partial list of specific embodiments of the present invention will be described, it is necessary to point out the following specific examples are only for the present invention will be further described, it does not mean to limit the scope of the present invention. 其他人根据本发明做出的一些非本质的修改和调整仍属于本发明的保护范围。 Others still fall within the scope of the invention, according to some non-essential modifications and adaptations of the present invention is made.

[0019] 实施例1 [0019] Example 1

含EGF的护肤凝胶: EGF-containing skin care gel:

组成上述护肤凝胶产品的组分及其百分数含量为: The percentage composition of the components and their contents skin gel product is:

EGF 0.0000005 EGF 0.0000005

水解胶原蛋白粉 0.20 Hydrolyzed collagen powder 0.20

透明质酸钠 0. 50 Sodium hyaluronate 0.50

丝肽粉 0.60 Silk peptide powder 0.60

棕榈酰五肽 1. 00 Palmitoyl Pentapeptide 1.00

肌肽 0. 20 Carnosine 0.20

卡波姆 0. 50 Carbomer 0.50

三乙醇胺 0. 25 Triethanolamine 0.25

甘油 14.00 Glycerin 14.00

防腐剂 0. 10 Preservatives 0.10

去离子水 余量 Deionized water Balance

上述护肤凝胶产品的制备方法,包括下列步骤: Skin gel products above preparation method, comprising the steps of:

(1)将卡波姆、透明质酸钠与甘油、去离子水混合,搅拌使其溶解; (1) carbomer, sodium hyaluronate and glycerin, deionized water, stirred and dissolved;

(2)将水解胶原蛋白粉、丝肽粉、棕榈酰五肽、肌肽、防腐剂分别加入以上溶液搅拌溶解均勻,然后加入EGF搅拌均勻; (2) hydrolyzing collagen powder, silk peptide powders, palmitoyl pentapeptide, carnosine, preservatives are added to the above solution and stirred to dissolve uniformly, followed by addition of EGF stir;

(3)三乙醇胺用水溶解成10%溶液; (3) Triethanolamine was dissolved in water to a 10% solution;

(4)将所配制的三乙醇胺溶液加入卡波姆甘油溶液,边加边搅拌,搅拌均勻后即得透明凝胶基质; (4) The prepared triethanolamine carbomer glycerol solution, with stirring, after stirring uniformly to obtain a transparent gel matrix;

(5)调节PH值至6. 8〜7. 5之间; (5) to adjust the PH value 8~7 between 6. 5;

(6)将上述凝胶进行检验,合格后,再进行灌装即得肌肤凝胶护肤品。 (6) The above gel test, after passing, and then filling to obtain a gel skin care products.

[0020] 实施例2 [0020] Example 2

含EGF的化妆水:组成上述化妆水产品的组分及其百分数含量为 EGF 0.0000008水解胶原蛋白粉 0. 50透明质酸钠 0. 02丝肽粉 0. 60棕榈酰五肽 0. 50肌肽 0. 21甘油 6. 00乙二胺四乙酸二钠 0. 10杰马防腐剂 0. 10氨甲基丙醇 0. 01去离子水 余量 EGF-containing lotion: content percentage composition of the components and the makeup of EGF .0000008 hydrolyzed fish collagen powder of sodium hyaluronate 0.02 0.50 0.60 Silk peptide powder Palmitoyl Pentapeptide 0.50 carnosine 0 21 glycerin 6.00 disodium edetate 0.10 preservative 0.10 Pierre aminomethyl propanol 0.01 deionized water balance

上述护肤化妆水产品的制备方法,包括下列步骤: Skin cosmetic preparation method of the fish, comprising the steps of:

(1)先将乙二胺四乙酸二钠、透明质酸钠、甘油、去离子水混合,搅拌溶解均勻; (1) First disodium edetate, sodium hyaluronate, glycerine, deionized water, uniformly stirred and dissolved;

(2)将水解胶原蛋白粉、丝肽粉、棕榈酰五肽、肌肽、防腐剂分别加入以上溶液搅拌溶解均勻; (2) hydrolyzing collagen powder, silk peptide powders, palmitoyl pentapeptide, carnosine, preservatives are added to the above solution and stirred to dissolve uniformly;

(3)再加入EGF搅拌均勻; (3) EGF added stir;

(5)加入氨甲基丙醇搅拌均勻,调节PH值至6. 5〜7. 5之间; (5) Stir aminomethyl propanol was added to adjust the PH value between 5 to 6. 5 ~ 7.;

(6)将上述化妆水过200目滤布后,进行检验,合格后,再进行灌装即得含EGF的化妆水护肤品。 (6) The above make-up water through a 200 mesh filter cloth, inspection, after passing makeup water filling and then obtain the EGF-containing skin care products.

[0021] 实施例3 [0021] Example 3

含EGF的润肤露: EGF-containing lotion:

组成上述润肤露的组分及其百分数含量为: The percentage composition of the components and their contents of lotion:

A组份: Component A:

PEG-100硬脂酸脂 2. 00 Lipid PEG-100 stearate 2.00

单甘油脂 1. 50 Monoglycerides 1.50

PEG-8 蜂蜡 1. 50 1.50 PEG-8 beeswax

聚二甲基硅氧烷 2.00 Polydimethylsiloxane 2.00

角鲨烷 4.00 Squalane 4.00

棕榈酸异丙酯 6. 00 Isopropyl palmitate 6.00

十六-十八醇 0. 50 SIXTEEN - octadecanol 0.50

尼泊金甲酯 0.20 Methyl paraben 0.20

尼泊金丙酯 0. 10 B组分: Propylparaben 0. 10 B Component:

去离子水 余量 Deionized water Balance

乙二胺四乙酸二钠 0.10 Disodium EDTA 0.10

甘油 5.00 Glycerin 5.00

汉生胶 0. 30 C组分:EGF 0.000001 Xanthan gum 0. 30 C components: EGF 0.000001

水解胶原蛋白粉 1.00 Hydrolyzed collagen powder 1.00

透明质酸钠 0. 30 Sodium hyaluronate 0.30

棕榈酰五肽 3. 50 Palmitoyl Pentapeptide 3.50

去离子水 1. 00 Deionized water 1.00

丝肽粉 1. 20 Silk peptide powder 1.20

肌肽 0.80 D组分: Carnosine 0.80 D component:

杰马防腐剂 0. 10 Jemma preservatives 0.10

香精 0.10 Flavor 0.10

上述润肤露的制备方法是: The method of the above-described lotion is prepared:

将B组分中的汉生胶分散均勻,溶解后,再将A组分、B组分分别加热到80°C后,两相混合均质数分钟,搅拌降温,降温到40°C,加入事先溶解均勻的C组分,搅拌均勻后加入D组分,搅拌均勻,35°C成白色细腻均勻的膏体后,进行检验,合格后,再进行灌装即得润肤露。 The xanthan gum is dispersed uniformly in component B, dissolved, and then component A, component B are heated separately to 80 ° C, the homogeneous two-phase mixture for several minutes, cooled with stirring, cooled to 40 ° C, was added prior after dissolution uniform component C, component D is added after mixing, stir, 35 ° C to a white fine uniform paste, inspection, after passing, and then filling to obtain lotion. [0022] 实施例4 含EGF的营养霜: [0022] EGF-containing embodiment of Example 4 Cream:

組成上述营养霜的组分及其百分数含量为: A组份: The percentage composition of the components and their nutritional content cream are: A Component:

PEG-100硬脂酸脂 2. 00 Lipid PEG-100 stearate 2.00

单甘油脂 2. 00 Monoglycerides 2.00

PEG-8 蜂蜡 2. 50 2.50 PEG-8 beeswax

聚ニ甲基硅氧烷 2.00 Poly ni dimethicone 2.00

角鲨烷 8.00 Squalane 8.00

棕榈酸异丙酯 6. 00 Isopropyl palmitate 6.00

白矿油 7.00 White mineral oil 7.00

十六-十八醇 1. 50 SIXTEEN - octadecanol 1.50

尼泊金甲酯 0.20 Methyl paraben 0.20

尼泊金丙酯 0. 10 B组分: Propylparaben 0. 10 B Component:

乙ニ胺四乙酸ニ钠 0.10 B ni ni tetraacetic acid 0.10 Sodium

去离子水 余量 Deionized water Balance

甘油 5. 00 Glycerin 5.00

丁ニ醇 5.00 5.00 ni butyl alcohol

汉生胶 0. 30 C组分: Xanthan gum 0. 30 C component:

EGF 0.0000015 EGF 0.0000015

水解胶原蛋白粉 1.50 Hydrolyzed collagen powder 1.50

透明质酸钠 0. 20 Sodium hyaluronate 0.20

棕榈酰五肽 2. 00去离子水 1. 00 Palmitoyl Pentapeptide 2.00 deionized water 1.00

丝肽粉 0.80 Silk peptide powder 0.80

肌肽 0.40 D组分: Carnosine 0.40 D component:

杰马防腐剂 0.10 Jemma preservatives 0.10

香精 0.10 Flavor 0.10

上述润肤露的制备方法是: The method of the above-described lotion is prepared:

将B组分中的汉生胶分散均勻,溶解后,再将A组分、B组分分别加热到80°C后,两相混合均质数分钟,搅拌降温,降温到40°C,加入事先溶解均勻的C组分,搅拌均勻后加入D组分,搅拌均勻,35°C成白色细腻均勻的膏体后,进行检验,合格后,再进行灌装即得润肤露。 The xanthan gum is dispersed uniformly in component B, dissolved, and then component A, component B are heated separately to 80 ° C, the homogeneous two-phase mixture for several minutes, cooled with stirring, cooled to 40 ° C, was added prior after dissolution uniform component C, component D is added after mixing, stir, 35 ° C to a white fine uniform paste, inspection, after passing, and then filling to obtain lotion.

[0023] 稳定性研究 [0023] Stability

对于在化妆品配方中EGF生物活性的保持情况,采用细胞增殖测量法测定EGF生物活性。 For the case of maintaining the biological activity of EGF in cosmetic formulations, the biological activity of EGF was measured by cell proliferation measurement. 细胞增殖方法是根据人表皮生长因子(hEGF)对小鼠胚胎成纤维细胞(Balb/c 3T3 细胞)的生长具有刺激作用,通过测量被活细胞中线粒体内的脱氢酶作用转化为甲臜(Formazan)的颜色的变化来表述EGF的效能。 Cell proliferation is a method of stimulating effect according to the human epidermal growth (of hEGF) on mouse embryonic fibroblasts (Balb / c 3T3 cells), growth factors, is converted by measuring dehydrogenase in viable cells to formazan in the mitochondria ( changes formazan) colors to express the EGF performance. BALB/C 3T3 No. A31是由SA Aaronson和GT Todaro于1968年从BALB/C小鼠胚胎上分离出来的细胞系之一。 BALB / C 3T3 No. A31 is one of the cell lines isolated from the SA Aaronson GT Todaro and in 1968 from the BALB / C mouse embryos out. 此类细胞的EGF受体,也可以与人表皮生长因子(hEGF)结合,进而会促进细胞的生长和分裂。 EGF receptor such cells, may be the human epidermal growth factor (of hEGF) binding, and thus will promote cell growth and division. 这个过程产生的代谢导致细胞内脱氢酶总体水平增加。 This metabolic process produces results in overall increased levels of intracellular dehydrogenase. 脱氢酶将反应底物MTS变成红色的甲臜,这可以通过测量含不同浓度hEGF的测试品在490 nm处吸收峰值来计算hEGF对于细胞生长的半数有效剂量(ED5tl)。 The dehydrogenase reaction substrate becomes red MTS formazan, which can be calculated from the absorption peak at 490 nm by measuring the test article containing different concentrations of hEGF hEGF for median effective dose (ED5tl) cell growth. 并由此半数有效剂量(ED5tl)来检测评价表皮生长因子的生物活性水平。 And thereby median effective dose (ED5tl) to detect the level of biological activity evaluation of epidermal growth factor.

[0024] 为证明该组合物在化妆品配方中有助于对EGF活性的保持,发明者使用上述实施例4制备得到的相应的产品,分别在25°C,40°C放置6个月加速老化实验,6个月后测试产品中的EGF相对活性,由此判定该组合物在化妆品配方中是否有助于EGF的活性保持,试用结果如图1所示。 [0024] To demonstrate that the composition facilitates retention of EGF activity, the inventors using the corresponding products described in the example 4 obtained, respectively at 25 ° C, 40 ° C for 6 months accelerated aging in cosmetic formulations experiment, six months after EGF relative activity of the test product, thereby determining the composition in cosmetic formulations it help to maintain the activity of EGF, trial results are shown in FIG. 由图1可见,该组合物在配方中明显有助于EGF活性的保持,而没加该组合物的对照组的EGF的活性随时间的变化,EGF活性消失很快。 As seen in FIG. 1, the composition formulation significantly helps to maintain the activity of EGF, EGF and did not increase the activity of the control group composition change with time, the activity of EGF soon disappear.

Claims (10)

1. 一种有助于保持表皮生长因子生物活性的化妆品组合物,其特征在于,该组合物的主要成分是表皮生长因子、水解胶原蛋白粉、透明质酸钠、丝肽粉、棕榈酰五肽和肌肽,辅助成分为化妆品外用剂型的常用基质以及去离子水或纯化水或蒸馏水。 An epidermal growth factor helps to maintain the biological activity of a cosmetic composition, characterized in that the main component of the composition is epidermal growth factor, hydrolyzed collagen powder, sodium hyaluronate, silk peptide powders, Palmitoyl Pentapeptide and peptide carnosine, cosmetic adjunct ingredients commonly used in topical form of a matrix and deionized water or purified water or distilled water.
2.根据权利要求1所述的保持表皮生长因子生物活性的化妆品组合物,其特征在于, 该组合物的组分的百分数含量为:表皮生长因子 0.0000001 〜0. 000003 水解胶原蛋白粉 0. 01 - -4. 00透明质酸钠 0. 01 - -3. 00丝肽粉 0. 01 - -5. 00棕榈酰五肽 0. 50 - -8. 00肌肽 0. 01 〜3. 00余量为化妆品外用剂型的常用基质以及去离子水或纯化水或蒸馏水。 The holder 1 Epidermal growth factor according to the biological activity of a cosmetic composition as claimed in claim, wherein the percentage amounts of the components of the composition are: epidermal growth factor 0.0000001 000003 ~ 0 0.01 hydrolyzed collagen powder. - sodium hyaluronate 0.01 -400 - -300 silk peptide powder 0.01 - -500 palmitoyl pentapeptide 0.50 - -8 00 00 carnosine 0.01 ~ 3 balance commonly used in topical form of cosmetic matrix and deionized water or purified water or distilled water.
3.根据权利要求1所述的保持表皮生长因子生物活性的化妆品组合物,其特征在于, 该组合物的组分的百分数含量为:表皮生长因子 0. 000001 〜0.000003水解胶原蛋白粉 0. 20 - -3. 00透明质酸钠 0. 10 - -2. 00丝肽粉 0. 10 - -3. 00棕榈酰五肽 1. 00 - -5. 00肌肽 0. 20 〜2. 00余量为化妆品外用剂型的常用基质以及去离子水或纯化水或蒸馏水。 The holder 1 Epidermal growth factor according to the biological activity of a cosmetic composition as claimed in claim, wherein the percentage amounts of the components of the composition are: epidermal growth factor 0.000001 ~0.000003 hydrolyzed collagen powder 0.20 - sodium hyaluronate 0.10 -300 - -200 silk peptide powder 0.10 - -300 palmitoyl pentapeptide 1.00 - -5 00 00 carnosine 0.20 ~2 balance commonly used in topical form of cosmetic matrix and deionized water or purified water or distilled water.
4.根据权利要求1所述的保持表皮生长因子生物活性的化妆品组合物,其特征在于, 该组合物的组分的百分数含量为:表皮生长因子 0.0000015水解胶原蛋白粉 1. 50透明质酸钠 0. 20丝肽粉 0. 80棕榈酰五肽 2. 00肌肽 0. 40余量为化妆品外用剂型的常用基质以及去离子水或纯化水或蒸馏水。 The holder 1 Epidermal growth factor according to the biological activity of a cosmetic composition as claimed in claim, wherein the percentage amounts of the components of the composition are: epidermal growth factor 0.0000015 hydrolyzed collagen powder of sodium hyaluronate 1.50 silk peptide powder 0.20 0.80 2.00 palmitoyl pentapeptide carnosine 0.40 cosmetics in topical form to balance common matrix and deionized water or purified water or distilled water.
5.根据权利要求1-4任一项所述的有助于保持表皮生长因子生物活性的化妆品组合物,其特征在于,所述水解胶原蛋白粉水解胶原蛋白粉是以深海鱼皮为原料经过净华提纯、 酶解、过滤、浓缩、喷雾干燥等处理而制成的粉末状产品,其分子量在500〜20000道尔顿之间,蛋白质含量要求大于等于90%。 According to claims helps maintain the biological activity of the epidermal growth factor cosmetic composition any of claims 1-4, wherein said hydrolyzed collagen, hydrolyzed collagen powder is a powder of raw material after deep skin Chinese net purification, enzymatic, filtered, concentrated, and spray drying process and made of powdered products, having a molecular weight between 500~20000 Dalton, requires a protein content greater than or equal to 90%.
6.根据权利要求1-4任一项所述的有助于保持表皮生长因子生物活性的化妆品组合物,其特征在于,所述透明质酸钠是由微生物发酵法生产制得,分子量控制在小于50万道尔顿,透明质酸钠的含量大于等于92%。 According to any of claims 1-4 according to facilitate retaining the biological activity of the epidermal growth factor cosmetic composition, wherein the sodium hyaluronate is obtained by the microbial fermentation production, molecular weight control less than 500,000 daltons, the content of sodium hyaluronate is not less than 92%.
7.根据权利要求1-4任一项所述的有助于保持表皮生长因子生物活性的化妆品组合物,其特征在于,所述丝肽粉是将蚕丝脱胶后的丝素经溶解、透析、提纯、降解、浓缩,喷雾干燥等处理而制成的分子量较小的丝蛋白制品,其分子量在2000以下,蛋白质含量大于等于90%。 The claims of claims 1-4 helps to maintain the biological activity of the epidermal growth factor cosmetic composition, characterized in that the silk fibroin peptide powder is dissolved by the degumming of silk, dialysis, purification, degradation, concentration, and spray drying process is made smaller molecular weight silk protein product, having a molecular weight of 2000 or less, the protein content of 90% or more.
8.根据权利要求1-4任一项所述的有助于保持表皮生长因子生物活性的化妆品组合物,其特征在于,所述棕榈酰五肽是指由赖氨酸-苏氨酸-苏氨酸-赖氨酸-丝氨酸这五种氨基酸按顺序组成的五肽的衍生物即棕榈酰-Lys-Thr-Thr-Lys-Ser。 According to claims it helps maintain the biological activity of the epidermal growth factor cosmetic composition any of claims 1-4, characterized in that said means palmitoyl pentapeptide lysine - Thr - Su leucine - lysine - serine amino acid sequence consisting of five pentapeptide derivatives i.e. palmitoyl -Lys-Thr-Thr-Lys-Ser.
9.根据权利要求1-4任一项所述的有助于保持表皮生长因子生物活性的化妆品组合物,其特征在于,所述的肌肽是指由丙氨酸和组氨酸组成的二肽,其含量大于等于99%。 According to claims it helps maintain the biological activity of the epidermal growth factor cosmetic composition any of claims 1-4, characterized in that said means dipeptide carnosine and histidine alanine consisting of in an amount greater than or equal to 99%.
10.根据权利要求1-4任一项所述的有助于保持表皮生长因子生物活性的化妆品组合物,其特征在于,所述的化妆品组合物为膏、霜、乳液、化妆水或凝胶。 According to any of claims 1-4 of the epidermal growth factor helps maintain the biological activity of a cosmetic composition, wherein said composition is a cosmetic cream, cream, lotion, gel or lotion .
CN2011103621240A 2011-11-16 2011-11-16 Cosmetic composition favorable for keeping biological activity of epidermal growth factor (EGF) CN102406556A (en)

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CN103070790A (en) * 2012-09-11 2013-05-01 苏州宝丽洁日化有限公司 Fragrance type makeup removing wet tissue
CN103340762A (en) * 2013-07-05 2013-10-09 上海珍馨化工科技有限公司 Hydrolyzed cocoon fiber toner
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CN103705415B (en) * 2013-12-13 2015-10-28 华南理工大学 Carrier containing EGF epidermal repair milk mesoporous silica and its preparation method
CN103948525A (en) * 2014-04-23 2014-07-30 广州宝生堂化妆品有限公司 Skin anti-ageing active composition
CN103948525B (en) * 2014-04-23 2016-04-06 广州宝生堂化妆品有限公司 An epidermal anti-active composition
CN104173212A (en) * 2014-09-10 2014-12-03 百莲凯国际企业管理(北京)有限公司 Regenerative cream and preparation technology thereof
CN104856892A (en) * 2014-11-14 2015-08-26 广州远想生物科技有限公司 Cosmetic or skin care product preparation rich in growth factors and preparation method therefor
CN104721067B (en) * 2015-03-13 2017-12-22 广州市白云联佳精细化工厂 An active compositions long-term storage of epidermal growth factor
CN104721067A (en) * 2015-03-13 2015-06-24 广州市白云联佳精细化工厂 Composition for preserving epidermal growth factor activity for long time
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CN106913510A (en) * 2017-04-20 2017-07-04 合肥卡迪尔化妆品有限公司 High-quality collagen mask
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