Background
The skin is used as the first barrier of human body, the skin is aged and aged all the time, the elasticity of the skin is slowly reduced, the skin becomes thinner, weaker and loose, and the visual impression of the skin is wrinkle and senile plaque. Skin aging is the prevention of gradual functional and organic degenerative changes in the skin over time, manifested by the prevention of wrinkles, dryness, scaling, sagging and discolorations. Anti-aging cosmetics are important functional cosmetics for realizing anti-aging effect.
The hydrolyzed collagen is prepared by a scientific processing method, can be completely dissolved in water relative to collagen polypeptide with small molecular weight, and has higher absorption and utilization rate by human bodies. The hydrolyzed collagen contains a plurality of amino acids, wherein the total amount of the amino acids is more than ninety percent of the hydrolyzed collagen by the maximum amount of the dry amino acids, and the secondary amino acids are alanine, glutamic acid, arginine, hydroxyproline and the like, and the content of the strong proline is up to 11.8-12.6 percent. These amino acids are important raw materials for the synthesis of autologous collagen by humans. Only when a human takes in enough amount of various amino acids, various proteins required by the human can be synthesized, the shape and structure of skin and tissues and organs are maintained, and various damaged tissues are repaired.
The polypeptide has various biological activities of scavenging free radicals, resisting oxidation, resisting aging and the like, can promote the proliferation of skin cells, can provide nutrition for skin, delays skin aging and promotes skin wound repair, and is widely applied to skin cosmetics and anti-aging cosmetics.
However, the efficacy of hydrolyzed collagen and polypeptides, particularly palmitoyl polypeptides, in lightening and removing freckles has not been described in the prior art. For example, patent document CN 109646324A describes a tightening and whitening spot-lightening cream extracted from collagen complex plant, which is prepared by mixing hydrolyzed collagen zinc, acetyl tyrosine, glutathione, alanine, valine, nicotinamide, squalane, xanthan gum, butylene glycol, carbomer, arbutin, citric acid, strawberry saxifrage extract, peony root extract, aminopropanol ascorbic acid phosphate, scutellaria membranaceus root extract, saposhnikovia divaricata root extract, rose oil, zanthoxylum piperitum fruit extract, usnea extract and deionized water. However, the skin can be moisturized and firmed by adding the collagen with small molecular weight, and the components with the effects of whitening and lightening the spots are acetyl-tyrosine, glutathione, arbutin, citric acid, a peony root extract, aminopropanol ascorbic acid phosphate and a divaricate saposhnikovia root extract. Patent document CN107714525A describes a specific essence for whitening and removing freckles, which includes metallothionein, tranexamic acid, hyaluronic acid, hydrolyzed collagen, top-level natural whitening moisturizing factors, amino acid moisturizers, licorice extract, olive oil, arbutin, lecithin wax, bitter orange flower essential oil, brown algae gel, and ceramide. However, the components which exert the whitening and spot-lightening effects are metallothionein, tranexamic acid, hyaluronic acid, liquorice extract and arbutin, and the spot-lightening effect of hydrolyzed collagen is not recorded. Patent document CN 109288753 a describes a whitening spot-lightening cream, which contains multiple oligopeptides, multiple plant extracts and a powerful humectant, and simultaneously, glabrous licorice anus and tranexamic acid, which are called whitening gold, are added to play a role in whitening and spot-lightening; the skin moisture can be supplemented, and the damaged skin can be repaired in time, so that the skin is white and red without stripes. As can be seen, although palmitoyl peptide is added in this document, the effect is only to reduce fine wrinkles, and the effects of spot lightening and spot removal, particularly the spot lightening effect for senile plaques, are not described.
Senile plaque is a skin degeneration of the elderly, and is represented by scattered pigmented plaque pieces which are different in size, flat or slightly higher than the skin surface, smooth in surface and tan in color. It is often seen in the exposed areas, such as the face, forehead, back of the hand, etc. The traditional method for removing senile plaque comprises CO2Laser, electric ion cauterization, freezing, grinding, surgical grafting, etc., but theseThe method is easy to produce adverse reactions such as scar and pigmentation. And the existing cosmetics are only rarely reported to the products for fading and removing the senile plaques, so that the research and development of the cosmetics with the obvious effects of fading and removing the senile plaques are urgently needed.
Disclosure of Invention
In view of the defects of the prior art, the invention aims to provide a peptide composition and application thereof, in particular to application of the peptide composition in preparing wrinkle-removing and/or spot-lightening skin-brightening products.
The purpose of the invention is realized by the following technical scheme:
in a first aspect, the invention provides a peptide composition, which comprises the following components in parts by weight: 0.2-4 parts of hydrolyzed collagen, 0.01-2 parts of palmitoyl polypeptide and 90-99 parts of solvent.
Preferably, the peptide composition comprises the following components in parts by weight: 1-1.5 parts of hydrolyzed collagen, 0.7-1.0 part of palmitoyl polypeptide and 95-98 parts of solvent.
Preferably, the palmitoyl polypeptide is at least one of palmitoyl dipeptide, palmitoyl tripeptide, palmitoyl pentapeptide, and palmitoyl hexapeptide.
Preferably, the palmitoyl polypeptide is palmitoyl tripeptide-5 or a mixture of palmitoyl tripeptide-5 and palmitoyl dipeptide-7.
Preferably, when the palmitoyl polypeptide is a mixture of palmitoyl tripeptide and palmitoyl dipeptide-7, the mixing weight ratio of the palmitoyl tripeptide and the palmitoyl dipeptide-7 is 6-8: 2-4.
Preferably, the hydrolyzed collagen is at least one of hydrolyzed fish collagen and hydrolyzed pig skin collagen. More preferably hydrolyzed pigskin collagen.
Preferably, the peptide composition also comprises 0.05 to 0.2 weight part of humectant; the humectant is selected from ethylhexyl glycerin;
the solvent is a mixture of water and polyhydric alcohol, and the mixing ratio of the water to the polyhydric alcohol is 70-90: 10-30;
the polyol is at least one selected from 1, 2-hexanediol and glycerol.
Preferably, the polyol is 1, 2-hexanediol. 1, 2-hexanediol has moisturizing and skin conditioning effects.
The peptide composition prepared by the invention has the effects of removing wrinkles, lightening spots and brightening skin, and particularly, when the combination of hydrolyzed pigskin collagen, palmitoyl tripeptide-5 and palmitoyl dipeptide-7 is adopted, more outstanding wrinkle removing effect and senile plaque lightening effect can be obtained.
In a second aspect, the invention provides an application of a peptide composition in preparing a spot-lightening and skin-brightening product, wherein the peptide composition comprises the following components in parts by weight: 0.2-4 parts of hydrolyzed collagen, 0.01-2 parts of palmitoyl polypeptide and 90-99 parts of solvent.
Preferably, the peptide composition comprises the following components in parts by weight: 1-1.5 parts of hydrolyzed collagen, 0.7-1.0 part of palmitoyl polypeptide and 95-98 parts of solvent.
Preferably, the palmitoyl polypeptide is at least one of palmitoyl dipeptide, palmitoyl tripeptide, palmitoyl pentapeptide, and palmitoyl hexapeptide.
Preferably, the palmitoyl polypeptide is palmitoyl tripeptide-5 or a mixture of palmitoyl tripeptide-5 and palmitoyl dipeptide-7.
Preferably, when the palmitoyl polypeptide is a mixture of palmitoyl tripeptide and palmitoyl dipeptide-7, the mixing weight ratio of the palmitoyl tripeptide and the palmitoyl dipeptide-7 is 6-8: 2-4.
Preferably, the hydrolyzed collagen is at least one of hydrolyzed fish collagen and hydrolyzed pig skin collagen. More preferably hydrolyzed pigskin collagen.
Preferably, the peptide composition also comprises 0.05 to 0.2 weight part of humectant; the humectant is selected from ethylhexyl glycerin;
the solvent is a mixture of water and polyhydric alcohol, and the mixing ratio of the water to the polyhydric alcohol is 70-90: 10-30;
the polyol is at least one selected from 1, 2-hexanediol and glycerol.
Preferably, the polyol is 1, 2-hexanediol. 1, 2-hexanediol has moisturizing and skin conditioning effects.
Preferably, the peptide composition is contained in the spot-lightening and skin-brightening product in an amount of 0.5-10%.
Preferably, the spot lightening and skin lightening product comprises an age spot lightening product and/or a skin lightening product.
In a third aspect, the invention also provides an application of the peptide composition in preparing a wrinkle-removing and/or speckle-lightening product, wherein the peptide composition comprises the following components in parts by weight: 0.2-4 parts of hydrolyzed collagen, 0.01-2 parts of palmitoyl polypeptide and 90-99 parts of solvent;
in the wrinkle-removing and/or speckle-lightening product, the content of the peptide composition is 0.5-10%.
Compared with the prior art, the invention has the following beneficial effects:
1. according to the invention, the hydrolyzed collagen and the palmitoyl polypeptide are compounded, so that the peptide composition prepared from the hydrolyzed collagen and the palmitoyl polypeptide has a good wrinkle removing effect, and also has the effects of spot lightening and skin brightening, and especially has an obvious effect of senile plaque lightening.
2. The invention further adopts the compounding of the palmitoyl tripeptide-5 and the palmitoyl dipeptide-7, combines the hydrolyzed pigskin collagen, and can further improve the wrinkle removing effect and remarkably improve the effects of senile plaque lightening and skin color brightening through the synergistic effect of the palmitoyl tripeptide-5 and the palmitoyl dipeptide-7.
Detailed Description
The present invention will be described in detail with reference to specific examples. The following examples will assist those skilled in the art in further understanding the invention, but are not intended to limit the invention in any way. It should be noted that variations and modifications can be made by persons skilled in the art without departing from the spirit of the invention. All falling within the scope of the present invention.
Example 1
The embodiment provides a peptide composition, which comprises the following components in parts by weight: 2 parts of hydrolyzed fish collagen, 51 parts of palmitoyl tripeptide, 87 parts of water and 10 parts of 1, 2-hexanediol.
The peptide composition is prepared by the following steps:
1) uniformly mixing 10 parts of water and 10 parts of 1, 2-hexanediol, and adding palmitoyl tripeptide-5 to obtain a clear and transparent solution A;
2) dissolving hydrolyzed fish collagen in 77 parts of water to obtain solution B;
3) and uniformly mixing the solution A and the solution B to obtain the clear and transparent peptide composition.
Example 2
The embodiment provides a peptide composition, which comprises the following components in parts by weight: 0.5 part of hydrolyzed fish collagen, 50.1 parts of palmitoyl tripeptide, 91.3 parts of water, 8 parts of 1, 2-hexanediol and 0.1 part of ethylhexyl glycerol.
The peptide composition is prepared by the following steps:
1) uniformly mixing 20 parts of water and 8 parts of 1, 2-hexanediol, and adding palmitoyl tripeptide-5 to obtain a clear and transparent solution A;
2) dissolving hydrolyzed fish collagen in 71.3 parts of water to obtain a solution B;
3) and uniformly mixing the solution A and the solution B to obtain the clear and transparent peptide composition.
Example 3
The embodiment provides a peptide composition, which comprises the following components in parts by weight: hydrolyzed pigskin collagen 1 part, palmitoyl tripeptide-50.7 parts, palmitoyl dipeptide-70.3 parts, water 87.5 parts, 1, 2-hexanediol 10 parts, and ethylhexyl glycerin 0.5 part.
The peptide composition is prepared by the following steps:
1) uniformly mixing 15 parts of water and 10 parts of 1, 2-hexanediol, and adding palmitoyl tripeptide-5 and palmitoyl dipeptide-7 to obtain a clear and transparent solution A;
2) dissolving hydrolyzed pigskin collagen in 72.5 parts of water to obtain solution B;
3) and uniformly mixing the solution A and the solution B, and then adding ethylhexyl glycerol and mixing to obtain the clear and transparent peptide composition.
Example 4
The embodiment provides a peptide composition, which comprises the following components in parts by weight: 1 part of hydrolyzed fish collagen, 50.7 parts of palmitoyl tripeptide, 70.3 parts of palmitoyl dipeptide, 87.5 parts of water, 10 parts of 1, 2-hexanediol and 0.5 part of ethylhexyl glycerol.
The peptide composition is prepared by the following steps:
1) uniformly mixing 15 parts of water and 10 parts of 1, 2-hexanediol, and adding palmitoyl tripeptide-5 and palmitoyl dipeptide-7 to obtain a clear and transparent solution A;
2) dissolving the hydrolyzed fish skin collagen in 72.5 parts of water to obtain a solution B;
3) and uniformly mixing the solution A and the solution B, and then adding ethylhexyl glycerol and mixing to obtain the clear and transparent peptide composition.
Example 5
The embodiment provides a peptide composition, which comprises the following components in parts by weight: hydrolyzed pigskin collagen 1 part, palmitoyl tripeptide-51 parts, water 87.5 parts, 1, 2-hexanediol 10 parts, and ethylhexyl glycerin 0.5 part.
The peptide composition is prepared by the following steps:
1) uniformly mixing 15 parts of water and 10 parts of 1, 2-hexanediol, and adding palmitoyl tripeptide-5 to obtain a clear and transparent solution A;
2) dissolving hydrolyzed pigskin collagen in 72.5 parts of water to obtain solution B;
3) and uniformly mixing the solution A and the solution B, and then adding ethylhexyl glycerol and mixing to obtain the clear and transparent peptide composition.
Example 6
The embodiment provides a peptide composition, which comprises the following components in parts by weight: hydrolyzed pigskin collagen 1 part, palmitoyl tripeptide-50.5 parts, palmitoyl dipeptide-70.5 parts, water 87.5 parts, 1, 2-hexanediol 10 parts, and ethylhexyl glycerin 0.5 part.
The peptide composition is prepared by the following steps:
1) uniformly mixing 15 parts of water and 10 parts of 1, 2-hexanediol, and adding palmitoyl tripeptide-5 and palmitoyl dipeptide-7 to obtain a clear and transparent solution A;
2) dissolving hydrolyzed pigskin collagen in 72.5 parts of water to obtain solution B;
3) and uniformly mixing the solution A and the solution B, and then adding ethylhexyl glycerol and mixing to obtain the clear and transparent peptide composition.
Example 7
The embodiment provides a peptide composition, which comprises the following components in parts by weight: hydrolyzed pigskin collagen 1 part, palmitoyl tripeptide-50.7 parts, palmitoyl pentapeptide-40.3 parts, water 87.5 parts, 1, 2-hexanediol 10 parts, and ethylhexyl glycerol 0.5 part.
The peptide composition is prepared by the following steps:
1) uniformly mixing 15 parts of water and 10 parts of 1, 2-hexanediol, and adding palmitoyl tripeptide-5 and palmitoyl pentapeptide-4 to obtain a clear and transparent solution A;
2) dissolving hydrolyzed pigskin collagen in 72.5 parts of water to obtain solution B;
3) and uniformly mixing the solution A and the solution B, and then adding ethylhexyl glycerol and mixing to obtain the clear and transparent peptide composition.
Example 8
The embodiment provides a peptide composition, which comprises the following components in parts by weight: hydrolyzed pigskin collagen 1.5 parts, palmitoyl tripeptide-50.5 parts, palmitoyl dipeptide-70.2 parts, water 87.5 parts, 1, 2-hexanediol 10 parts, and ethylhexyl glycerin 0.5 part.
The peptide composition is prepared by the following steps:
1) uniformly mixing 15 parts of water and 10 parts of 1, 2-hexanediol, and adding palmitoyl tripeptide-5 and palmitoyl dipeptide-7 to obtain a clear and transparent solution A;
2) dissolving hydrolyzed pigskin collagen in 72.5 parts of water to obtain solution B;
3) and uniformly mixing the solution A and the solution B, and then adding ethylhexyl glycerol and mixing to obtain the clear and transparent peptide composition.
Example 9
The embodiment provides a peptide composition, which comprises the following components in parts by weight: hydrolyzed pigskin collagen 1.2 parts, palmitoyl tripeptide-50.6 parts, palmitoyl dipeptide-70.4 parts, water 87.3 parts, 1, 2-hexanediol 10 parts, and ethylhexyl glycerin 0.5 part.
The peptide composition is prepared by the following steps:
1) uniformly mixing 15 parts of water and 10 parts of 1, 2-hexanediol, and adding palmitoyl tripeptide-5 and palmitoyl dipeptide-7 to obtain a clear and transparent solution A;
2) dissolving hydrolyzed pigskin collagen in 72.3 parts of water to obtain solution B;
3) and uniformly mixing the solution A and the solution B, and then adding ethylhexyl glycerol and mixing to obtain the clear and transparent peptide composition.
Example 10
The embodiment provides a peptide composition, which comprises the following components in parts by weight: 0.5 part of hydrolyzed pigskin collagen, 50.7 parts of palmitoyl tripeptide, 70.3 parts of palmitoyl dipeptide, 88 parts of water, 10 parts of 1, 2-hexanediol and 0.5 part of ethylhexyl glycerol.
The peptide composition is prepared by the following steps:
1) uniformly mixing 15 parts of water and 10 parts of 1, 2-hexanediol, and adding palmitoyl tripeptide-5 and palmitoyl dipeptide-7 to obtain a clear and transparent solution A;
2) dissolving hydrolyzed pigskin collagen in 73 parts of water to obtain solution B;
3) and uniformly mixing the solution A and the solution B, and then adding ethylhexyl glycerol and mixing to obtain the clear and transparent peptide composition.
Example 11
The embodiment provides a peptide composition, which comprises the following components in parts by weight: hydrolyzed pigskin collagen 1 part, palmitoyl tripeptide-50.35 parts, palmitoyl dipeptide-70.15 parts, water 88 parts, 1, 2-hexanediol 10 parts, and ethylhexyl glycerin 0.5 part.
The peptide composition is prepared by the following steps:
1) uniformly mixing 15 parts of water and 10 parts of 1, 2-hexanediol, and adding palmitoyl tripeptide-5 and palmitoyl dipeptide-7 to obtain a clear and transparent solution A;
2) dissolving hydrolyzed pigskin collagen in 73 parts of water to obtain solution B;
3) and uniformly mixing the solution A and the solution B, and then adding ethylhexyl glycerol and mixing to obtain the clear and transparent peptide composition.
Example 12
The embodiment provides a peptide composition, which comprises the following components in parts by weight: 1 part of hydrolyzed pigskin collagen, 71 parts of palmitoyl dipeptide, 88 parts of water, 10 parts of 1, 2-hexanediol and 0.5 part of ethylhexyl glycerol.
The peptide composition is prepared by the following steps:
1) uniformly mixing 15 parts of water and 10 parts of 1, 2-hexanediol, and adding palmitoyl tripeptide-1 to obtain a clear and transparent solution A;
2) dissolving hydrolyzed pigskin collagen in 73 parts of water to obtain solution B;
3) and uniformly mixing the solution A and the solution B, and then adding ethylhexyl glycerol and mixing to obtain the clear and transparent peptide composition.
Comparative example 1
The comparative example provides a peptide composition comprising the following components in parts by weight: hydrolyzed pigskin collagen 1 part, palmitoyl tripeptide-10.7 parts, palmitoyl dipeptide-70.3 parts, water 87.5 parts, 1, 2-hexanediol 10 parts, and ethylhexyl glycerin 0.5 part.
The peptide composition is prepared by the following steps:
1) uniformly mixing 15 parts of water and 10 parts of 1, 2-hexanediol, and adding palmitoyl tripeptide-1 and palmitoyl dipeptide-7 to obtain a clear and transparent solution A;
2) dissolving hydrolyzed pigskin collagen in 72.5 parts of water to obtain solution B;
3) and uniformly mixing the solution A and the solution B, and then adding ethylhexyl glycerol and mixing to obtain the clear and transparent peptide composition.
Comparative example 2
The comparative example provides a peptide composition comprising the following components in parts by weight: hydrolyzed pigskin collagen 1 part, palmitoyl tripeptide-50.7 parts, palmitoyl tetrapeptide-70.3 parts, water 87.5 parts, 1, 2-hexanediol 10 parts, and ethylhexyl glycerol 0.5 part.
The peptide composition is prepared by the following steps:
1) uniformly mixing 15 parts of water and 10 parts of 1, 2-hexanediol, and adding palmitoyl tripeptide-5 and palmitoyl tetrapeptide-7 to obtain a clear and transparent solution A;
2) dissolving hydrolyzed pigskin collagen in 72.5 parts of water to obtain solution B;
3) and uniformly mixing the solution A and the solution B, and then adding ethylhexyl glycerol and mixing to obtain the clear and transparent peptide composition.
Effect verification:
1. wrinkle removing effect
The experimental method comprises the following steps:
selecting the left and right canthus parts, and performing by an instrument evaluation method. The age of the testees is 25-50 years, the number of the testees is 60, and the testees are divided into 6 groups on average. The face is cleaned on the test day, the moisture is absorbed by paper towels, and the face is dried and balanced for 20-30 min in a constant temperature and humidity chamber (the temperature is 20 +/-2 ℃ and the relative humidity is 30% -60%). The positions of the left and right canthi are marked, and the canthus wrinkles before use are photographed and analyzed by using a skin microscope VC98 and an active skin surface analysis system SELS.
Using samples after cleaning every morning and evening, the left canthus of 6 groups of subjects were smeared with experimental samples 1-6 (sample matrix comprising 0.3% U21, 0.3% methyl paraben, 0.3% triethanolamine, balance water) containing the peptide compositions prepared in example 2, example 3, example 5, example 12, comparative example 1, comparative example 2 at a concentration of 5%, respectively, and the right canthus of two groups of subjects were smeared with a matrix control sample containing no peptide composition, each time the size of mung bean was evenly smeared in the test area and kept dry. After 2, 4, 6, 8 weeks of use, the test day after the cleaning balance the control pre-use area was again photographed using the skin microscope VC98 and the active skin surface analysis system SELS for the corner wrinkles and analyzed for changes in SEw.
The experimental results are as follows:
the effect of experimental samples 1 and 2 on skin wrinkles was calculated by calculating the stroma control test value at each time point as 100% based on the test value before use. Significant differences were calculated with the method of TTest after use compared to before use, P <0.05, P < 0.01. The calculation formula is as follows:
a) the change rate%
b) Relative change% (% change in sample group/change in matrix group × 100%)
The evaluation results of skin wrinkles SEw using VC98 are shown in table 1 (results are average values). The experimental sample containing 5% peptide composition began to decrease in skin wrinkle parameter SEw 2 weeks after use, with a significant difference after 6 weeks of use, compared to before use (time 0 weeks). After 8 weeks of continuous use, the skin wrinkles are obviously reduced, and the reduction rate can reach 80.2% at least. Thus, it was demonstrated that skin wrinkles can be improved by using a peptide composition containing 5% of the peptide of the present invention for a long period of time.
TABLE 1
2. Speckle reducing effect
The experimental method comprises the following steps:
subjects aged 55-75 years and having obvious senile plaques were randomly divided into 15 groups of 5 persons each. Subjects applied each day after cleansing their face in the morning and evening the area size of the senile plaques in the test area after applying each of the experimental samples 1-12 (sequentially corresponding to 5% of the peptide composition prepared in examples 1-12, using a sample matrix comprising 0.3% U21, 0.3% methyl paraben, 0.3% triethanolamine, and the balance water) and the comparative samples 1-2 (sequentially corresponding to 5% of the peptide composition prepared in comparative examples 1-2, using a sample matrix comprising 0.3% U21, 0.3% methyl paraben, 0.3% triethanolamine, and the balance water) to the test area, continuously for 8 weeks, before application and after applying the experimental samples for 2, 4 and 8 weeks. And the same test was performed using the sample matrix without the peptide composition as a control sample.
The experimental results are as follows:
calculating the change rate (%) of the area of the senile plaque according to the change of the area of the senile plaque before and after use, wherein the calculation formula is as follows:
senile plaque area change rate (%) gamma 100%
The results obtained are shown in table 2 (average values), and the peptide composition prepared by hydrolyzing collagen and palmitoyl tripeptide-5 or palmitoyl dipeptide-7 (sample 1/2/12) has a certain senile plaque-lightening effect; when the palmitoyl tripeptide-5 and palmitoyl dipeptide-7 are compounded, the senile plaque lightening effect of the prepared peptide composition is obviously improved; particularly, when the hydrolyzed pigskin collagen with specific content and the palmitoyl tripeptide-5 and the palmitoyl dipeptide-7 with specific proportion are adopted, the effect of remarkably lightening the senile plaques can be obtained. While there was little or no effect of lightening age spots when compounded with other types of palmitoyl polypeptides (comparative examples 1 and 2). Therefore, the invention can obtain very obvious spot-lightening effect through the coordination of the selection of specific palmitoyl polypeptide types and the compounding of the palmitoyl polypeptide types and hydrolyzed collagen, particularly hydrolyzed pigskin collagen.
FIG. 1 is a photograph showing a comparison of senile plaques of one subject before and after application of the test sample 2. Fig. 2 is a photograph showing a comparison of senile plaques of one subject before and after application of the experimental sample 3. As can be seen from fig. 1 and 2, the effect of lightening age spots of the smear test sample 3 is significantly better than that of the smear test sample 2.
TABLE 2
3. Skin lightening effect
The experimental method comprises the following steps:
subjects aged 25-50 years were randomized into 15 groups of 5 individuals each. Subjects applied each test sample 1-12 (sequentially containing 5% of the peptide composition prepared in examples 1-12, a sample base comprising 0.3% U21, 0.3% methyl paraben, 0.3% triethanolamine, and the balance water) and comparative sample 1-2 (sequentially containing 5% of the peptide composition prepared in comparative examples 1-2, a sample base comprising 0.3% U21, 0.3% methyl paraben, 0.3% triethanolamine, and the balance water) to the subject site after daily cleansing of the face in the morning and evening, for 8 weeks, and skin color was measured 2, 4, and 8 weeks before application (0 weeks) and after application with the test samples. And the same test was performed using the sample matrix without the peptide composition as a control sample.
Skin chromaticity values of L, a, b of the skin at the test site were measured separately using a skin colorimeter, three times for each test area, and the ITA ° values were recorded and calculated. The larger the ITA DEG value of the tested skin area, the lighter the skin color and vice versa.
The experimental results are as follows:
the results are shown in table 3, and the peptide composition prepared by the invention has obvious skin lightening effect. Particularly, when the palmitoyl tripeptide-5 and palmitoyl dipeptide-7 are compounded with hydrolyzed collagen, the skin color can be remarkably lightened through the coordination effect of the palmitoyl tripeptide-5 and the palmitoyl dipeptide-7.
TABLE 3
4. Safety test
The experimental method comprises the following steps:
this experiment researchThe safety of each peptide composition was evaluated by examining the toxicity of the peptide composition obtained in each example to human primary fibroblast NHF cells after the peptide composition acted on the cells. Taking NHF cells growing in logarithmic phase, discarding culture solution, washing with PBS for 2 times, adding 0.05% pancreatin, standing at 37 deg.C for 5min, stopping digestion with DMEM complete culture solution containing 10% FBS and 1% PS, collecting cells, centrifuging at 1000rpm for 3min, discarding supernatant, resuspending cells with complete culture solution, counting, and adjusting cell concentration to 1 × 105Each well was 100. mu.l/ml in a 96-well plate. After 24h, the cells are attached, and 100. mu.l of the test sample with the peptide composition concentration ranging from 1% to 10% after dilution is added to each well. Mixing, and further incubating in the incubator for 24 + -1 h.
The experimental results are as follows: the peptide composition prepared in all the examples has no obvious toxicity to cells and no safety problem at the concentration of 1-10%.
The invention has many applications, and the above description is only a preferred embodiment of the invention. It should be noted that the above examples are only for illustrating the present invention, and are not intended to limit the scope of the present invention. It will be apparent to those skilled in the art that various modifications can be made without departing from the principles of the invention and these modifications are to be considered within the scope of the invention.