KR20190037094A - Skin external composition for promoting skin keratin turnover - Google Patents

Abstract

The present invention relates to a composition for external application for skin, which comprises polypeptides derived from Jeju green soybean (scientific name: Glycine max, generic name: soybean, cultivar name: Pureunkong) and sodium lactate as an active ingredient. The composition is excellent in promoting skin keratin turnover and also has an effect of reducing the amount of skin keratin and improving skin texture, skin tone, skin transparency and application uniformity of make-up cosmetics.

Description

(Skin external composition for promoting skin keratin turnover)

The present invention relates to a method for promoting keratinocyte turnover by containing a polypeptide derived from Jeju pea fermented product and sodium lactate, more precisely, a Jeju peach ( Glycine max , generic name: Pureunkong) The present invention also relates to a composition for external application for skin, which is excellent in the effect of reducing skin mass, skin texture, skin tone, transparency and make-up uniformity.

The stratum corneum of the skin is the outermost layer in direct contact with the outside, protecting the skin and the body from external hazards. The stratum corneum accounts for 50% of the protein called keratin, and the rest is composed of 20% fat, 23% water-soluble material and 7% moisture. Skin cells continue to be produced, pushing existing skin cells to the stratum corneum gradually, where the cells reaching the stratum corneum are flat and flat dead cells, also called 'keratin'. In the skin, millions of dead dead keratin cells fall apart and replaced with new keratinocytes.

This change in position of the new cell layer in the epidermis is called turnover. Depending on area and age, it takes about 4 weeks for this skin to turn over in normal skin. If these stratum corneum remain on the surface of the skin without peeling normally, the stratum corneum becomes thicker and the face light becomes dark and dark. In addition, impurities remaining on the surface of the skin or hair follicles may be oxidized or decomposed by oxygen or microorganisms, causing skin troubles such as inflammation.

Alpha Hydroxy Acids (AHA) is a typical chemical exfoliating ingredient, which helps remove the connective ring between the stratum corneum and the stratum corneum by the action of acid, and has the effect of gently dissolving keratin. Particularly, the components corresponding to AHA are glycolic acid, lactic acid, malic acid, citric acid, tartaric acid and the like. Among them, lactic acid It is used as a natural moisturizing factor (NMF) in cosmetics.

Jeju peach ( Glycine max , generic name: Pureunkong) is called "purin poisonous bean" in Jeju, which means blue egg-shaped bean. It is also called 'Jangkong' when used to soak the cabbage. Jeju is a native resource produced only in the southern part of Jeju Island. It is difficult to preserve the seeds and it is in danger of extinction. It grows taller than general beans and is vulnerable to winds. It has a longer growing period. If it is planted on land rather than Jeju, its quality and composition may be lowered. Jeju peanut contains much more isoflavone than regular beans. Traditional miso sauce, which is soaked with Jeju pea, has many different fungi, fungi and enzymes that are produced from nature compared to the modified soybean paste that is sold in the market. It is known to have excellent efficacy.

The present inventors have found through studies and experiments that a polypeptide derived from Jeju pea kee promotes collagen gene expression in skin cells and has an effect on cell regeneration. It has also been found that the use of sodium lactate as a moisturizing agent as a natural moisturizing factor can exert an excellent skin horny turnover promoting effect on the polypeptide derived from the Jeolla ginkgo in order to complete the present invention.

 1. Internet document "Do you know Jeju Peak" (internet url; https://farmer-story.kakao.com/farmer-story/pureunbeans)  2. Analysis of Protein Composition of Domestic and Herbicide Resistant Soybean (Chun Hua Wei et al., "Protein Composition of Domestic and Glyphosate-Tolerant Soybean", Journal of the Korean Society of Food Science and Nutrition v.35 no.4, 2006, pp.470 - 475)

In the present invention, in addition to obtaining the conventionally known effects of the soybean-derived peptides and sodium lactate, which are effects of improving the elasticity and moisturizing effect, the two components of Jeju peanut-derived polypeptide and sodium lactate are used together to promote the skin horny turnover To provide an excellent composition for skin external application. It is also intended to provide a composition for external application for skin which is excellent in effect of reducing mass, skin texture, skin tone, transparency, and make-up uniformity. The present invention also relates to the use of a mixture of Jeju peanut, more precisely a peptide derived from Jeju peanut fermentation product and sodium lactate, more particularly a skin corneal turnover promoting and / or skin texture, , Transparency, and make-up uniformity of a mixture of Jeju pea derived polypeptides and sodium lactate.

The present invention relates to a skin care composition which contains Jeju peanut derived polypeptide and sodium lactate at a specific ratio and which is excellent in accelerating skin corneal turnover effect and having skin angle mass reduction effect, skin texture, skin tone, transparency and makeup uniformity improvement effect Thereby providing an external application composition. A low molecular weight peptide prepared by fermentation of Bacillus subtilis in liquid fermentation and ultrafiltration process is used as a polypeptide derived from Jeolla peat.

The composition for external application for skin according to the present invention is excellent in the exfoliation ability of the skin, and has a superior horny turnover promoting effect by reducing the exfoliation turnover cycle. Also, it does not disappear and remains on the skin for a long time to reduce the amount of keratin which causes the skin to become dull or cause troubles, and shows the effect of improving skin texture, skin tone, transparency and makeup uniformity.

Fig. 1 is a photograph of each mass obtained before use of the composition for external application for skin of Example 1, 3 days after use, 1 week after use, and 2 weeks after use using Visioscan VC98.
Fig. 2 is a photograph of the keratin before the use of the composition for external application of the skin of Example 1, 3 days after use, 1 week after use, and 2 weeks after use using i-Scope.
FIG. 3 is a photograph of the skin external application composition prepared in Example 1 to examine the keratin peel ability of the composition, after applying fluorescent dyes to the horny part of the test piece, and then applying the sample to the fluorescence image. The dense appearance of fluorescence means that the exfoliation does not occur relatively well. (Left of each photograph: non-embossed, right of each photograph: when applying Example 1)
FIG. 4 shows the results of analyzing the amino acid components of the peptides derived from the peony root of the present invention using liquid chromatography (UPLC).
FIG. 5 is a graph showing the composition of the amino acid content of the Jeolla ginkgo-derived polypeptide according to the present invention as a percentage.
Fig. 6 shows the results of the dipeptide analysis of the peptides derived from Jeolla ginseng according to the present invention, which was analyzed by LC-ESI-MS. FIG. 6A shows the total ion current, and FIG. 6B shows the ion (Leu / Val-related peptide ion) extracted from the total ion current.

The present invention relates to a skin care composition comprising Jeju peanut derived polypeptide and sodium lactate as an active ingredient and having excellent effect of accelerating skin horny turnover and having skin angle mass reduction effect, skin texture, skin tone, transparency and makeup uniformity improvement effect And an external preparation composition.

The soybean-derived peptide or the hydrolyzed soybean protein has been conventionally known to have anti-aging and moisturizing effects. The present inventors have confirmed through experiments that the polypeptide derived from Jeju pea keong promotes collagen gene expression and is effective for cell regeneration.

In addition, sodium lactate is a sodium salt of lactic acid and is widely used in moisturizing cosmetics. It is known to act as a humectant as one of natural moisturizing factors (NMF).

In the present invention, the Jeju pea derived polypeptide and sodium lactate are used at the same time to increase the exfoliating effect and accelerate skin horn turnover. The existing exfoliating products mainly depend on exfoliation function on the epidermis. However, for the more fundamental exfoliation, activation of new cell formation and differentiation in the skin granules and basal layer as well as exfoliation on the epidermis can promote turnover and maintain skin homeostasis.

The present inventors have confirmed that a polypeptide derived from Jeju pea keon promotes collagen gene expression and cell regeneration in skin cells. And it was predicted that it would accelerate skin keratin turnover when it was combined with sodium lactate. It has been predicted that improved skin regeneration will actively push healthy cells onto the skin to reduce turnover cycles and help maintain skin homeostasis after exfoliation. The synergy effect was confirmed, and the present invention was completed.

The Jeju sweet potato-derived polypeptide used in the composition for external application of the skin according to the present invention is obtained by fermenting Jeju sweet potato as a Jeju native plant produced in the southern part of Jeju Island by liquid fermentation with Bacillus subtilis and decomposing into low molecular peptide, . More specifically, Jeju peanut is crushed and filtered, and the filtered Jeju peanut is added to purified water, followed by fermentation with Bacillus subtilis, followed by filtration through an ultrafiltration membrane, concentrating the filtrate, and spray drying to finally obtain a powdery peel- Can be obtained.

In the present invention, the peanut derived peptides are low molecular weight peptides having an average molecular weight of less than 3,000 Da. The low molecular weight peptide preferably has an average molecular weight of 1,000 Da to 2,500 Da, more preferably 1,000 Da to 1,500 Da.

The composition for external application for skin according to the present invention may contain 0.000001 to 5% by weight, based on the total weight of the composition, of the Jeju peanut derived polypeptide as a dry weight. If the content is less than 0.000001% by weight, the content is too low and it is difficult to expect the effect as an external preparation for skin, and if it exceeds 5% by weight, there is a fear of skin safety. More specifically, it is preferably contained in an amount of 0.005 to 0.05% by weight.

The composition for external application for skin according to the present invention may contain sodium lactate in an amount of 0.000001 to 20% by weight based on the total weight of the composition. If the content is less than 0.000001% by weight, the content is too low and it is difficult to expect the effect as an external preparation for skin. If the content is more than 20% by weight, there is a fear of skin safety. More specifically, it is preferably contained in an amount of 1 to 10% by weight.

The composition for external application for skin according to the present invention has a skin cornea turnover promoting effect. It also has effects of reducing mass, skin texture, skin tone, transparency, and makeup uniformity.

In the composition according to the present invention, it is preferred that mixing the Jeolla ganoderma derived polypeptide and sodium lactate at a mixing ratio of 1: 100 to 1: 2000, preferably 1: 100 to 1: 500 on the basis of weight, Effect of at least one of skin texture, skin tone, transparency, and make-up uniformity.

The composition for external application for skin according to the present invention may be in the form of a solution, a gel, a solid, a paste anhydrous product, an emulsion obtained by dispersing an oil phase in a water phase, a suspension, a microemulsion, a microcapsule, a microgranule or an ionic (liposome) In the form of a dispersant, or in the form of a skin, lotion, cream, pack, gel, powder, ointment or spray.

The composition for external application for skin according to the present invention can be used in the form of a skin, an organic solvent, a solubilizer, a thickening agent, a gelling agent, a softening agent, an antioxidant, a suspending agent, a stabilizer, a foaming agent, a fragrance, The active ingredient may be combined with any other ingredients commonly used in cosmetics, such as ionic emulsifiers, fillers, sequestering agents, chelating agents, preservatives, vitamins, blockers, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, And may further contain adjuvants conventionally used in the same cosmetic or dermatological field.

The composition for external application for skin according to the present invention may further contain additional additives conventionally used in the art in addition to the above-mentioned components.

Hereinafter, the present invention will be described in more detail by way of Examples and Test Examples. However, the following examples and test examples are provided only to aid understanding of the present invention, and the present invention is not limited thereto.

[Referential Example 1] Preparation of polypeptides derived from Jeju peanut

The peptides derived from the Jeju pea kerosene used in the present invention were prepared by fermenting Bacillus subtilis in Jeju alpine produced in Jeongmun-dong, Seogwipo city, Jeju, Korea, and applying a microfiltration process to prepare low molecular weight peptides having an average molecular weight of less than 3,000 Da . Specifically, 4 kg of Jeju pea pods, which were ground and filtered with 100 mesh, were added to 40 kg of purified water, 40 g of Bacillus subtilis was added, and the mixture was fermented at 50 ° C for 6 hours, deactivated at 90 to 95 ° C for 30 minutes And cooled to 50 to 60 ° C. The filtrate was concentrated at 60 ° C and finally spray-dried (condition Stirring speed 30 rpm, Input 185 ° C, Output 85 to 90 ° C). The filtrate was filtered through a 10 kDa ultrafiltration membrane, To obtain a powdery peptides derived from Jeju pea ke.

[Referential Example 2] Preparation of Peptide Derived from White Soybean

Peptide derived from Baekun bean was prepared through the same procedure as in [Referential Example 1], except that Jeju peach was replaced with white bean.

[Referential Example 3] Production of Peptide Derived from Pungsan Soybean

The peptides derived from the peonies were obtained through the same procedure as in [Referential Example 1], except that the Jeju peas were replaced with the peonies.

[Reference Example 4] Sodium lactate

Sodium lactate was PURASAL S / PF 60 from Corbion.

[Preparation of Examples and Comparative Examples]

Examples 1 to 3, which contained the peptides derived from the Jeju pea kerks of Reference Example 1, the sodium lactate of Reference Example 4, Comparative Example 1, which contained peptides derived from Baekun soybean and sodium lactate, Peeling cream of Comparative Example 2 containing Peptide derived from Jeju pea kerosene and Potassium lactate and Peeling cream of Comparative Example 3 containing Peptide derived from Poongsan bean derived peptide of Reference Example 3 and sodium lactate was prepared according to the composition shown in Table 1 below: weight%)

ingredient Example 1 Example 2 Example 3 Comparative Example 1 Comparative Example 2 Comparative Example 3 Peptide derived from Jeju peanut 0.01  0.01 0.005 0 0.01 0 Pea derived from white bean soybean 0 0 0 0.01 0 0 Peptide derived from poison soybean 0 0 0 0 0 0.01 Sodium lactate 10 5 10 10 0 10 Potassium lactate 0 0 0 0 10 0 Squalane 5 5 5 5 5 5 Polysorbate 60 0.5 0.5 0.5 0.5 0.5 0.5 Cetearyl alcohol 1.5 1.5 1.5 1.5 1.5 1.5 glycerin 2 2 2 2 2 2 Purified water to 100 to 100 to 100 to 100 to 100 to 100 Sum 100 100 100 100 100 100

The effects of improving the mass, skin texture, skin tone, skin transparency, makeup uniformity and promoting skin horny turnover of the prepared examples and comparative compositions were confirmed through human body application tests.

The subjects were 25 to 35 years old (mean age: 29.391 ± 3.313). The final validity was 23, and the number of dropouts was 2. The test period lasted for a total of 4 weeks (before use, 3 days after use, 7 days after use, 2 weeks after use, 4 weeks after use), and the above Example and Comparative Example creams were applied once daily And tested.

[Test Example 1: skin angle mass measurement]

Visioscan VC98 (Courage-Khazaka electronic GmbH, Germany) was used to measure the skin mass, and it was measured before use of the peeling cream of Example 1 and 3 days after use, 1 week after use, and 2 weeks after use. The corneal epithelium was collected by using a special film (Corneofix), and images were taken with Visioscan VC98 and analyzed. The measured value DI (Desquamation Index) Respectively.

Where A is the percentage of the area covered with keratinocytes, Tn is the percentage of keratinocytes associated with thickness, and n is the level of thickness (1 to 5).

The average value of the resulting D.I. and the number of improved subjects are shown in Tables 2 and 3, respectively.

medium Before use After 3 days of use After 1 week of use After 2 weeks of use Each mass (D.I) 18.413 16.951 15.983 15.481

Number of improved subjects (%) Before use - After 3 days of use Before use - After 1 week of use Before use - After 2 weeks of use Each mass (D.I) 17 (73.9) 20 (87.0) 20 (87.0)

As shown in the above Table 2, the average value of the mass DIs was 7.940%, 13.197%, and 15.924% after 3 days of use, 1 week after use, and 2 weeks after use, (P <0.017), respectively.

Also, as shown in Table 3, the number of subjects having improved the respective mass D.I scores was 73.9% after 3 days of use, 87% after 1 week and 87% after 2 weeks of use.

Thus, it was confirmed that the composition for external application for skin according to the present invention is excellent in reducing the respective masses.

[Test Example 2: Visual evaluation of each mass (each mass + keratin mature visual evaluation)]

Each mass enhancement visual evaluation was carried out using i-Scope before the peeling cream of Example 1 and after 3 days of use, 1 week after use, 2 weeks after use, and visual evaluation of skin mass and keratin maturity.

Table 4 shows the average value of each of the mass visual evaluation scores, the average value of the maturation visual evaluation scores, and the sum of the scores of the obtained mass visual evaluation scores, and the number of improved test subjects is shown in Table 5.

medium Before use After 3 days of use After 1 week of use After 2 weeks of use Each mass visual evaluation 2.087 2.435 2.826 3.261 Evaluation of keratin maturity 1.870 2.217 2.174 1.957 Horny visual evaluation (summed) 3.957 4.652 5.000 5.217

Number of improved subjects (%) Before use - After 3 days of use Before use - After 1 week of use Before use - After 2 weeks of use Each mass visual evaluation 10 (43.5) 16 (69.6) 19 (82.6) Evaluation of keratin maturity 7 (30.4) 10 (43.5) 8 (34.8) Horny visual evaluation (summed) 13 (56.5) 16 (69.6) 17 (73.9)

As shown in Table 4, the average value of each visual inspection score was significantly increased by 35.410% and 56.253% (p < 0.017) after one week of use and two weeks after use, respectively. There was no significant difference in the average value of keratin mature visual assessment score compared with before use. The cumulative score (each visual and horny maturation visual evaluation) was significantly increased (p <0.017) after 3 days of use, 1 week after use, 17.564%, 26.358%, and 31.842% after 2 weeks of use compared to before use.

Also, as shown in Table 5, the ratio of improved number of test subjects was 56.5% at 3 days, 69.6% at 1 week, and 73.9% at 2 weeks after use.

Therefore, it can be confirmed that the composition for external application for skin according to the present invention has many testers judged to be effective in mass reduction when evaluated visually.

[Test Example 3: Measurement of makeup uniformity]

To measure the makeup uniformity, the face was measured using Visia-CR (Canfield Imaging Systems, USA), and the same amount of makeup product was applied to the face before and 3 days after the peeling cream of Example 1 was used. After specifying a specific area of the photographed image, the standard deviation of the intensity, which means skin uniformity of the area, was analyzed using Image-pro plus. The decrease in the standard deviation value of the intensity means that the makeup uniformity is improved.

The average value of the resulting make-up uniformity (Intensity's std) is shown in Table 6, and the number of improved test subjects is shown in Table 7.

medium Before use After 3 days of use Makeup Uniformity (Intensity's std) 5.922 5.399

Number of improved subjects (%)
(Before use - after 3 days of use) Makeup Uniformity (Intensity's std) 22 (95.7)

As shown in Table 6, the mean value of the makeup uniformity (std of intensity) when using the composition for external application according to the present invention was significantly decreased (p <0.05) by 8.831% after 3 days of use as compared with before use.

Also, as shown in Table 7, the number of test subjects whose makeup uniformity was improved was very high at 95.7% after 3 days of use.

Accordingly, it was confirmed that the composition for external application for skin according to the present invention is excellent in the make-up uniformity improvement effect.

[Test Example 4: skin texture, skin tone, skin transparency measurement]

The skin texture was measured using the 3D skin imaging system PRIMOS premium (GFMesstechnik GmbH, Germany). The measurements were taken at the same site and the skin Ra values of the stored images were analyzed. Ra means Average Roughness, which means that the skin texture is improved as the value decreases.

Skin tones were measured using the Visia-CR (Canfield Imaging Systems, USA). After specifying a specific area of the photographed image, L * value indicating the skin brightness of the area was analyzed using Image-pro plus. This means that as the L * value of the image increases, the skin tone improves.

Skin transparency was measured using Translucency (TLS850). The Alpha value was used as the skin transparency evaluation data when the translucency probe was applied to the skin 3 times. The lower the Alpha value, the better the skin transparency.

Each was measured before use of the peeling cream of Example 1 and 3 days after use and one week after use.

The average values of skin roughness (Ra), skin tone (L *) and skin transparency (Alpha) obtained as a result are shown in Table 8, and the number of improved subjects is shown in Table 9.

medium Before use After 3 days of use After 1 week of use Skin texture (Ra) 13.090 12.603 12.276 Skin tone (L *) 66.815 67.425 68.061 Transparency (Alpha) 0.033 0.032 0.032

Number of improved subjects (%) Before use - After 3 days of use Before use - After 1 week of use Skin texture (Ra) 17 (73.9) 19 (82.6) Skin tone (L *) 22 (95.7) 22 (95.7) Transparency (Alpha) 20 (87.0) 20 (87.0)

As shown in Table 8, the average value of skin roughness (Ra) was 6.218% (p < 0.05) after 1 week of use compared to before use when using the composition for external application according to the present invention. The mean value of skin tone (L *) was significantly increased (p <0.025) by 0.913% and 1.865% after 3 days of use compared to before use. The mean value of skin transparency (Alpha) was 1.220% and 1.524% significantly decreased (p <0.05) 3 days after use and 1 week after use compared to before use.

As shown in Table 9, the number of subjects having improved skin texture, skin tone, and transparency was 82.6%, 95.7%, and 87.0%, respectively, after 1 week of use.

Therefore, it was confirmed that the composition for external application for skin according to the present invention is excellent in improving skin texture, skin tone and transparency.

[Test Example 5: Measurement of horny turnover effect]

The measurement of the horny turnover effect was made by evaluating the exfoliating ability of keratin in Examples 1 to 3 and Comparative Examples 1 to 3 prepared above. This study was conducted on 23 women aged 25 to 35 years. The test period was 4 weeks. The evaluation method was as follows:

1. After washing the entire area, the area was selected as 4 cm 2.

2. 100 μl of Dansyl chloride (in triglyceride oil) was loaded on a filter paper having a diameter of 20 mm and then spread on the test site.

3. After stabilization for 24 hours after confirming fluorescent staining, 32 ㎕ of sample was applied to the test site once a day (one sample was applied by rolling 20 times).

4. Daily light was blocked and stratum corneum fluorescence was taken under UV.

<Photography Conditions>

In digital camera manual mode, aperture 3.5, shutter speed 5

<Evaluation method of peelability>

- After dyes were fluorescently dyed with dansyl chloride, the exfoliation ability of the product was analyzed by decreasing the fluorescence intensity.

Significant differences were analyzed using continuous t-test (p <0.05), ANOVA test and technical statistical analysis, and the results are shown in Tables 10 and 11 and FIG.

Intensity Average Before use After 1 week of use After 2 weeks of use After 4 weeks of use Martial arts 188.425 109.459 94.722 90.839 Example 1 186.410 101.081 92.320 66.524 Example 2 186.562 103.283 92.043 67.452 Example 3 187.933 103.582 92.723 69.324 Comparative Example 1 189.345 114.532 94.806 82.235 Comparative Example 2 186.356 124.457 93.875 81.245 Comparative Example 3 187.259 119.533 94.974 87.253

Number of improved subjects (%) Before use - After 1 week of use Before use - After 2 weeks of use Martial arts 17 (73.9) 14 (60.9) Example 1 23 (100.0) 23 (100.0) Example 2 23 (100.0) - Example 3 22 (95.6) - Comparative Example 1 17 (73.9) - Comparative Example 2 18 (78.2) - Comparative Example 3 17 (73.9) -

As shown in Table 10, when the composition of Example 1 was used, the average value of the fluorescence intensity of keratin removing ability was 45.775% after 1 week of use, 50.475% after 2 weeks of use, 64.313% after 4 weeks of use, (P < 0.05). Example 2 significantly reduced (p <0.05) from 44.638% after 1 week of use, 50.664% after 2 weeks of use and 63.845% after 4 weeks of use, whereas Example 3 was 44.883% after 1 week of use, 50.662 %, And 63.112% after 4 weeks of use (p <0.05).

In contrast, in Comparative Example 1, the Intensity value was significantly reduced (p <0.05) after 39.51% of the first week, 49.929% after 2 weeks, 56.569% , 49.626% at 2 weeks after use, 56.403% at 4 weeks after use, and 36.167% at 1 week after use, 49.282% at 2 weeks after use, 53.405% after 4 weeks of use (P < 0.05).

Intensity values without application of the compositions of the examples and the comparative examples were significantly reduced (p <0.05) by 41.908% after 1 week, 49.730% after 2 weeks, and 51.790% after 4 weeks of use, respectively.

Examples 1 to 3 showed a significant difference (p < 0.05) before use, after 1 week of use, after 2 weeks of use, after 4 weeks of use, and significant differences compared to Comparative Examples 1 to 3 (p < 0.05). In particular, after 4 weeks of use, the intensity reduction rates of Examples 1 to 3 were about 11.3% to about 12.5% higher than those of the non-coated type, and about 6.5% to 10.9% higher than those of Comparative Examples 1 to 3. This shows that the exfoliating ability of the embodiment according to the present invention shows the most pronounced effect at the point of 4 weeks after the ordinary turnover cycle.

As shown in Table 11, the number of subjects to be improved in the evaluation of keratin peel ability was as high as 100% after one week of use and two weeks after use as compared with before using the composition of Example 1. Also, the composition of the compositions of Example 2 and Example 3 showed a very high ratio of 100% and 95.6%, respectively, after 1 week of use compared to before use.

FIG. 3 is a photograph showing fluorescence images obtained by applying fluorescent dyes to the keratin materials of the area coated with Example 1 and the non-coated area, and then irradiating the sample with fluorescence to examine the exfoliation performance of the external composition for skin according to the present invention. It means that the exfoliation does not occur relatively well. It can be seen from FIG. 3 that the fluorescence intensity of the composition of Example 1 is significantly reduced after one week of use compared to non-application.

Therefore, the results of Tables 10 to 11 and FIG. 3 show that when the compositions of Examples 1 to 3 containing the Jeju peanut derived polypeptide and sodium lactate were used, there was. In addition, the above Examples 1 to 3 are superior to Comparative Examples 1 and 3 containing peptides derived from other soybean peptides in place of the Jeju peanut derived polypeptide, and to Comparative Example 2 containing potassium lactate in place of sodium lactate, In particular, it was found that the peel-off performance of Example 1 containing 0.01% by weight of peach-derived peach-derived polypeptide and 10% by weight of sodium lactate was the best. As a result, it was confirmed that the composition for external application for skin containing the peanut-derived peptides and sodium lactate according to the present invention is excellent in the exfoliating ability of the keratin and promotes the skin horny turnover.

[Test Example 6] Amino acid analysis of polypeptides derived from Jeju pea

The amino acid components contained in the final powder-form Jeju pea derived polypeptide prepared in Referential Example 1 were analyzed by Ultra Performance Liquid Chromatography (Waters UPLC H-Class, Waters Co. Ltd., USA).

The chromatograms analyzed by the UPLC are shown in FIG. 4, and the amino acid contents of the peptides derived from Jeju pea kwon according to the amino acid analysis were as shown in Table 12 below. FIG. 5 shows a graph of the percentage conversion.

amino acid content
(μg / mg) Isoleucine 9.18 Phenylalanine 7.30 Tyrosine 5.08 Methionine 5.06 Glutamic acid 4.82 Leucine 4.57 Serine 3.67 Alanine 3.57 Lysine 2.99 Arginine 2.88 Glycine 2.67 Theanine 2.60 Asparagine 2.55 Aspartic acid 2.43 Tryptophan 2.32 Proline 2.28 Histidine 1.60 Glutamine 0.75 Cystine 0.73 GABA 0.68 Valine 0.23 Total content 67.96

From the results shown in Table 12 and FIG. 5, it was confirmed that isoleucine (13.5%) and phenylalanine (10.7%) were the most abundant in the peptides derived from Jeju peanut over 10 μg / mg.

According to the above amino acid analysis results, it is understood that the polypeptide of Jeju alpine root is very rich in isoleucine as compared with other bean of domestic varieties. Analysis of Protein Composition of Domestic and Herbicide Resistant Soybean (Chun Hua Wei et al., Protein Composition of Domestic and Glyphosate-Tolerant Soybean , Journal of the Korean Society of Food Science and Nutrition v.35 no.4, 2006, pp. 470 - 475), the amino acid composition of the peonies is richest in glutamine (15.54%), followed by phenylalanine (9.00%) and glycine (8.50%). In this document, the amino acid composition ratio of the other domestic soybean such as golden brown bean and soybean soy bean is also described as being similar to that of poison soybean except for tryptophan and pyrrolamine. In contrast, according to the present invention, the content of glutamine was low in 1.1% of the peptides derived from Jeolla peat, while the content of isoleucine was 13.5%, which is superior to other domestic varieties.

[Test Example 7] Dipeptide analysis of polypeptides derived from Jeju kelp

The final powder-form Jeju pea-derived polypeptide prepared in Referential Example 1 was pretreated and analyzed for peptide ions by LC-ESI-MS. 1 ml of 1 ml of distilled water per 1,000 ml of the sample was added to a 1.5 ml glass vial, sonicated for 10 minutes, centrifuged at 13,000 rpm at 4 ° C for 20 minutes, and 100 μl of the supernatant was analyzed. The pretreated supernatant was analyzed by Liquid Chromatography Mass Spectrometry (Thermo Q Exxtive Plus, Thermo Fisher Scientific, USA).

The total ionic current of the Jeju pea derived polypeptides analyzed by the LC-ESI-MS is shown in FIG. 6A. From this total ion current, Leu / Val-associated dipeptide ions in the range of m / z 231.16942 to 231.17061 were extracted. The results are shown in FIG. 6B, and the extracted Leu / Val-related peptide ion content is shown in Table 5 below. Val-Ile and Val-Leu were relatively quantified using Ile-Val standards.

RT [M + H] &lt; ⁺ Identification Peak area Concentration (μg / ml) Content (%) of polypeptides derived from Jeju peanut 2.33 231.17047 Ile-Val 1.092E + 08 0.25 0.0025 2.70 231.16948 Val-Ile 2.723E + 08 0.57 0.0057 3.10 231.17039 Val-Leu 5.214E + 08 1.06 0.0106

Through the ion analysis, it was confirmed that the Val-Leu, Val-Ile and Ile-Val dipeptides, which are one kind of the Leu-Val dipeptide group, are contained at a high concentration of 106, 57 and 25 ppm, respectively Respectively.

Claims (7)

A cosmetic composition for accelerating horny turnover comprising as an active ingredient a polypeptide derived from Jeju alpine ( Glycine max , generic name: Pureunkong, variety name: Pureunkong) and sodium lactate. The cosmetic composition for promoting skin horny turnover according to claim 1, wherein the peptides derived from the Jeolla peat is a low molecular weight peptide having an average molecular weight of less than 3,000 Da. [Claim 3] The cosmetic composition for accelerating skin keratinocyte turnover according to claim 2, wherein the Jeolla peat derived polypeptide is obtained by fermenting Jeju peanut with Bacillus subtilis in liquid phase followed by ultrafiltration. The cosmetic composition for accelerating horny keratinous turnover according to claim 1, wherein the content of the peanut derived peptides is 0.000001 to 5 wt% based on the total weight of the composition. The cosmetic composition for promoting skin horny turnover according to claim 1, wherein the content of sodium lactate is 0.000001 to 20% by weight based on the total weight of the composition. A cosmetic composition for improving make-up uniformity, comprising as an active ingredient, a polypeptide derived from Jeju alpine ( Glycine max , generic name: Pureunkong, variety name: Pureunkong) and sodium lactate. A cosmetic composition for improving skin texture, skin tone, and transparency, comprising as an active ingredient, a polypeptide derived from Jeju alpaca (generic name: Glycine max , generic name: Pureunkong, variety name: Pureunkong) and sodium lactate.

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