KR101871927B1 - Skin external composition containing sugar apple seed extract - Google Patents

Abstract

The present invention relates to a composition for external application for skin containing an extract of sugar apple, and more particularly, to a composition for external application for skin comprising an extract of sugar apple seeds germinated or a sugar apple seed extract using a subcritical extraction method, The present invention relates to a composition for external application for skin, which provides an extract having a higher content of cyclic peptides and various amino acids than that of an apple seed extract and can exert higher efficacy by using the extract.

Description

[0001] The present invention relates to a skin external composition containing sugar apple seed extract,

The present invention relates to a composition for external application for skin containing an extract of sugar apple, and more particularly, to a composition for external application for skin comprising an extract of sugar apple seeds germinated or a sugar apple seed extract using a subcritical extraction method, The present invention relates to a composition for external application for skin, which provides an extract having a higher content of cyclic peptides and various amino acids than an extract of apple seeds and can exert higher efficacy by using the extract.

As the aging progresses, the content and arrangement of collagen, elastin, hyaluronic acid and glycoprotein, which constitute the skin, are changed or decreased, and oxidative stress due to free radicals and active noxious oxygen is experienced. In addition, most of the cells constituting the skin undergo aging or ultraviolet rays, Cox-2 (cyclooxygenase), an enzyme that produces a proinflammatory cytokine known to cause inflammation, And the biosynthesis of matrix metalloproteinase (MMP), which is an enzyme that degrades skin tissue, is increased by these inflammatory factors, and nitric oxide (NO) production by iNOS (inducible nitric oxide synthase) Respectively. In other words, there is a decrease in cell activity due to natural endogenous aging and a decrease in the biosynthesis of substrate materials due to microinflammation, an increase in stress due to various harmful environments, and an increase in active coral species caused by sunlight As the degradation and denaturation are accelerated, the skin substrate is destroyed and thinned, and various symptoms of skin aging appear. Therefore, it is a reality that many researches have been conducted on the active ingredients which can prevent and improve such aging phenomena.

Korean Patent Publication No. 2011-0125247

Therefore, the present inventors have found that the extract of Sugar Apple Seed can provide an excellent anti-aging effect in natural extracts. Especially, the extract of Sugar Apple Seed germinated has a higher content of amino acids than Sugar Apple Seed, The present inventors have found that the use of the critical extraction method can extract more various kinds and a large amount of the efficacious ingredient and can provide an excellent anti-aging effect.

Accordingly, it is an object of the present invention to provide a composition for external application for skin which shows various skin improving effects including an anti-aging effect by containing sugar extract.

To achieve the above object, the present invention provides a composition for external application for skin comprising an extract of germinated sugar apple seed as an active ingredient.

The present invention also provides a composition for external application for skin comprising an extract of Sugar Apple Seed prepared by a subcritical extraction method as an active ingredient.

Also, the present invention provides an anti-aging external preparation for skin comprising an extract of Sugar Apple Seed germinated or a Sugar Apple Seed Extract prepared by a sub-critical extraction method as an active ingredient.

The composition according to the present invention contains a larger amount of amino acid components than the sugar extract obtained by conventional extraction methods using germinated sugar apple seed extract or sub-critical extraction method, The biosynthesis enhancing effect is excellent and a higher anti-aging effect can be provided.

The composition for external application for skin of the present invention contains an extract of Sugar Apple Seed as an active ingredient.

Sugar apple seeds used in the present invention are seeds of Sugar Apple tree. Sugar apple is known as Anona Cherimolia, Anona Squamosa, Anona Reticulata and Anona Muricata.

Sugar Apple fruit is a small lump of 2-4 inches with soft creamy flesh, sweet and mint-like as custard, and 1 cm black seed in the fruit.

Sugar apple seeds contain eight cyclic peptides, which are known to enhance the elasticity of the skin to impart lifting efficacy and inhibit inflammatory cytokines. Examples of such treatments include treatment of skin diseases and ringworm It has been used in various folk remedies from.

Sugar apple seed extract used in the present invention is extracted from sugar beet seeds, preferably from germinated sugar beet seeds. In case of germinated sugar apple, it contains more active ingredient needed for life phenomenon as compared to the state before germination, so that it can provide stronger effect.

The germination conditions of sugar apple seeds are not particularly limited, but they can be germinated by the following method. Put the seeds in water at 21 ~ 27 ℃ temperature condition and keep for 5 ~ 8 hours. The seeds are immersed in water and germinated by repeated treatment of light and dark conditions at a temperature of 25 to 30 ° C. At this time, the light condition is treated for 16 ~ 18 hours, the dark condition is treated for 5 ~ 7 hours, and the culture is continued for 5 ~ 7 days.

In addition, the composition of the present invention contains an extract of Sugar Apple Seed produced by Subcritical Water Extraction as an active ingredient. At this time, Sugar Apple can use both pre-germinated Sugar Apple and germinated Sugar Apple seeds.

In conventional supercritical extraction methods, the density of supercritical fluids is similar to that of liquids, and viscosity and diffusion coefficients are similar to those of gases. Therefore, when a supercritical fluid is used as an extraction solvent, it can be easily penetrated into a micro-particle of a plant and has an appropriate molecular density, so that a desired substance extract can be obtained by surrounding molecules of a desired substance and coming out. At this time, as a substance which can be used as an extraction solvent by making it into a supercritical state, it should first be non-toxic, and since the supercritical state is high temperature / high pressure, there is no danger of explosion and excellent dissolving power is required as an extraction solvent. Once these conditions are met, any substance can be made into a supercritical fluid for extraction.

On the other hand, the subcritical technology used in the present invention is used as an extraction solvent in a state in which the solvent does not exceed the critical point, and can perform the same performance as supercritical extraction depending on what solvent is selected. In the present invention, Water is used. Since supercritical carbon dioxide can not extract polar substances, organic solvent should be used as an auxiliary solvent. Supercritical carbon dioxide, however, is superior in extraction performance and range of operating conditions is large. Therefore, extraction of both nonpolar and polar materials It is possible. In addition, in the case of extraction with subcritical water, a hydrolysis reaction can also proceed.

Sugar apple seed extract by the subcritical extraction method used in the present invention can be specifically prepared as follows. Sugar apple seeds are prepared, and sugar components are extracted at a high pressure of 50 to 200 MPa, preferably 100 MPa using a subcritical extraction device to extract an active ingredient. The effective components are not sufficiently extracted at a pressure of less than 50 MPa and the increase in the extraction of the active ingredient is insignificant compared with the high pressure at a pressure exceeding 200 MPa. After extraction, the filter is filtered once with a filter having a pore size of 1.2 mu m, once with a filter having a pore size of 0.45 mu m, and once with a filter having a pore size of 0.2 mu m to remove impurities and microorganisms to prepare a subcritical sugar apple seed extract .

The composition of the present invention is preferably used in an amount of 0.01 to 95.0% by weight, preferably 5.0 to 95.0% by weight, based on the total weight of the composition, of germinated sugar apple seed extract or subcritical sugar apple seed extract prepared by the above- May be contained in an amount of 10.0 to 90.0% by weight. If the content of the extract is less than 0.01% by weight, it is difficult to expect the skin effect of the ingredient. If the content of the extract is more than 95.0% by weight, the efficacy of the extract is not greatly increased.

The composition of the present invention enhances collagen synthesis, thereby enhancing skin elasticity and improving wrinkles, thereby realizing an excellent anti-aging effect.

In addition, the composition of the present invention can provide an excellent antioxidant ability, can improve the overall condition of the skin in addition to the anti-aging effect, and can provide a skin whitening effect, skin moisturizing power improving effect, anti-inflammatory effect, It is possible to provide an overall improvement effect on the overall skin condition such as improvement effect, sebum control effect, pore contraction effect, keratinization effect, skin convergence or tidiness effect, improvement of complexion through improvement of blood circulation, skin irritation mitigation effect, .

The composition according to the invention may be formulated containing a cosmetically or dermatologically acceptable medium or base. These are all formulations suitable for topical application, for example as a solution, a gel, a solid, a paste anhydrous product, an emulsion obtained by dispersing an oil phase in water, a suspension, a microemulsion, a microcapsule, a microgranule or an ionic form (liposome) In the form of ionic fibrin dispersions, or in the form of creams, skins, lotions, powders, ointments, sprays or conical sticks. It can also be used in the form of a foam or in the form of an aerosol composition further containing a compressed propellant. These compositions may be prepared according to conventional methods in the art.

In addition, the composition according to the present invention may further comprise at least one selected from the group consisting of fatty substances, organic solvents, solubilizers, thickening agents, gelling agents, softening agents, antioxidants, suspending agents, stabilizing agents, Such as fillers, emulsifiers, emulsifiers, fillers, sequestering agents, chelating agents, preservatives, vitamins, blockers, moisturizers, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, lipid vesicles or any other ingredient commonly used in cosmetics May contain adjuvants commonly used in the field of cosmetics or dermatology. Such adjuvants are introduced in amounts commonly used in the cosmetics or dermatological fields.

In addition, the composition of the present invention may contain a skin absorption promoting substance to increase the anti-aging effect.

Hereinafter, the constitution and effects of the present invention will be described in more detail with reference to test examples and formulation examples. However, these test examples and formulation examples are provided for illustrative purposes only for the sake of understanding of the present invention, and the scope and scope of the present invention are not limited by the following examples.

[Example 1] Preparation of general germinated sugar apple seed extract

The germinated sugar apple seeds were placed in a 70% purified water and 30% alcohol solution and left at 25 ° C for 6 hours to extract active ingredients. After the extraction, the filter was filtered once with a filter having a diameter of 3 μm and once with a filter having a diameter of 1 μm to remove foreign matter. Finally, a microorganism was removed with a filter to obtain a sugar apple seed extract.

[Example 2] Preparation of subcritical sugar apple seed extract

Sugar apple seeds, which are not germinated, were extracted with a subcritical extraction device at a high pressure of 100 MPa. After extraction, the extract was filtered once with a filter having a pore size of 1.2 mu m, once with a filter having a pore size of 0.45 mu m, and once with a filter having a pore size of 0.2 mu m to remove impurities and microorganisms.

[Example 3] Preparation of subcritical germinated sugar apple seed extract

The germinated sugar apple seeds were extracted by using a subcritical extraction device at a high pressure of 100 MPa. After extraction, the extract was filtered once with a filter having a pore size of 1.2 mu m, once with a filter having a pore size of 0.45 mu m, and once with a filter having a pore size of 0.2 mu m to remove impurities and microorganisms.

[Comparative Example 1] Production of general sugar apple seed extract

Sugar apple seeds, which are not germinated, were put into 70% purified water and 30% alcohol and left at 25 ℃ for 6 hours to extract the active ingredients. After the extraction, the filter was filtered once with a filter having a diameter of 3 μm and once with a filter having a diameter of 1 μm to remove foreign matter. Finally, a microorganism was removed with a filter to obtain a sugar apple seed extract.

[Test Example 1] Comparison of extraction method of sugar apple seed extract and content of active ingredient according to germination state

Sugar apple seed extracts of Examples 1 to 3 and Comparative Example 1 were analyzed by amino acid analysis method (OPA method) as follows. The results are shown in Table 1 below.

<Amino acid analysis method (OPA method)>

1) HPLC conditions

- Column: zorbax column (amino acid analysis only)

- Mobile:

A = 1.36 g Sodium acetate trihydrate + 100 μl Triethylamine → 500 ml Conjugation → pH 7.2 (adjusted with acetic acid) → THF 1.5 ml

B = 1.36 g Sodium acetate trihydrate / 100 ml H ₂ O → pH 7.2 + Methanol 200 ml + ACN 200 ml

- Flow rate: 0.5 ml / min

- Injection: injection program for online derivatization *

- Detector: 338 nm

- Gradient: Online program *

2) Reagent preparation

- Amino acid standard solution: Aspartic acid, glutamic acid, proline, glycine, alanine, valine, etc. 10 mg / 100 ml H ₂ O

- OPA Reagent: reagent supplied by HP Company, 6 month shelf life, ampoule form

- Borate buffer: Supplied in 100ml unit, needed for amino acid color development

amino acid Content (μg / mL) Common germinated sugar apple seed extract
(Example 1) Subcritical Sugar Apple Seed Extract
(Example 2) Subcritical sprouting sugar apple seed extract
(Example 3) Common sugar apple seed extract
(Comparative Example 1) Asp 0.53 0.56 0.69 0.31 Glu 0.51 0.53 0.88 0.27 Ser 0.40 0.47 0.53 0.33 His 0.38 0.35 0.26 - Gly 0.37 0.36 0.97 0.17 The 0.26 0.23 0.38 - Arg 0.33 0.31 0.41 0.11 Ala 0.25 0.26 0.33 0.13 Tyr 0.37 0.38 0.38 0.22 Cys 1.31 1.33 1.56 0.53 Val 0.24 0.26 0.59 - Met 0.47 0.47 0.75 0.51 Phe 0.38 0.34 0.20 0.31 Ile 0.21 0.22 0.35 0.21 Leu 0.34 0.34 0.63 0.19 Lys 0.42 0.45 0.58 0.32 Pro 0.56 0.51 0.65 0.52 Total 7.33 7.37 10.14 4.13

As can be seen from Table 1, when the amino acid content of the Sugar Apple Seed Extract (Comparative Example 1) obtained using the general extraction method was regarded as 100%, the germinated sugar apple seed extract (Example 1) or the subcritical extract The total amino acid content of the sugar-apple seed extract (Example 2) prepared by the method of the present invention was increased to 178%. In particular, Sugar apple seed extract (Example 3) using the germinated seeds was 246% Amino acid content was increased.

Therefore, it can be confirmed that the germinated sugar apple seed extract or subcritical germinated sugar apple seed extract used in the present invention contains more potent components than the conventional common sugar apple seed extract.

[Referential Example 1] Preparation of Formulation Examples 1 to 3 and Comparative Formulation Example 1

Lotions of Formulation Examples 1 to 3 and Comparative Formulation Example 1 were prepared according to the composition shown in Table 2 below.

division Raw material name Formulation Example 1 Formulation Example 2 Formulation Example 3 Comparative Formulation Example 1 Comparative Formulation Example 2 Awards Common germinated sugar apple seed extract
(Example 1) 10 - - - - Subcritical Sugar Apple Seed Extract
(Example 2) - 10 - - - Subcritical sprouting sugar apple seed extract
(Example 3) - - 10 - - Common sugar apple seed extract
(Comparative Example 1) - - - 10 - Purified water To 100 To 100 To 100 To 100 To 100 Disodium iodide 0.02 0.02 0.02 0.02 0.02 glycerin 5.0 5.0 5.0 5.0 5.0 Xanthan gum 0.15 0.15 0.15 0.15 0.15 Methylglucose-20 2.0 2.0 2.0 2.0 2.0 Pentyleneglycol 5.0 5.0 5.0 5.0 5.0 Glycereth-26 2.0 2.0 2.0 2.0 2.0 Ammonium acryloyldimethyltaurate / Vpicopolymer 0.3 0.3 0.3 0.3 0.3 Ethanol phase ethanol 3.0 3.0 3.0 3.0 3.0 Hydrogenated castor oil 0.3 0.3 0.3 0.3 0.3 incense 0.1 0.1 0.1 0.1 0.1 antiseptic 0.3 0.3 0.3 0.3 0.3

<Formulation Example and Manufacturing Method of Comparative Formulation Example>

(1) The aqueous phase components are homogeneously mixed at room temperature.

(2) Ethanol phase components are heated (50-55 ° C) to dissolve and mix uniformly.

(3) The above-mentioned (1) is added to the above-mentioned (2) under agitation so as to be solubilized uniformly.

[Test Example 2] Skin elasticity improvement effect

In order to test the effect of improving the elasticity in the skin of an actual person, the above-mentioned Formulation Examples 1 to 3 and Comparative Formulation Examples 1 to 2 were used as follows. The skin elasticity was measured using a skin elasticity tester (Cutometer SEM 575, C + K Electronic Co., Germany) after applying morning and evening twice daily for 4 weeks. And the results are shown in Table 3 below. The results in the table indicate the viscoelasticity of the skin elasticity meter.

Test substance Skin elasticity effect Formulation Example 1 0.48 0.10 Formulation Example 2 0.47 + 0.10 Formulation Example 3 0.53 + - 0.10 Comparative Formulation Example 1 0.29 ± 0.09 Comparative Formulation Example 2 0.20 ± 0.09

As shown in the results of Table 3, Comparative Formulation Example 1 containing the common Sugar Apple Seed extract showed a skin elasticity improvement effect of about 150% as compared to Comparative Formulation Example 2 containing no Sugar Apple Seed extract. However, when Formulation 1 containing ginger sugar apple seed extract was used and Formulation Example 2 containing sub-critical sugar apple seed extract was used, 250% of Comparative Example 2 and 165% of Comparative Formulation Example 1 In the case of Formulation 3 containing subcutaneous germinated sugar apple seed extract, the skin elasticity was improved by 265% compared to Comparative Formulation 2, and the best efficacy was shown.

[Test Example 3] Collagen synthesis promotion effect

Human fibroblasts were cultured in a 24-well plate incubator and then replaced with a medium containing common sugar apple seed extract and germinated sugar apple seed extract as shown in Table 4. On the third day of culture, 0.5 ml of DMEM medium containing 10% fetal bovine serum was added to each well, and then 10 μCi of L [2,3,4,5- ³ H] -proline was added. After 24 hours, the media and cells in each well were scraped off, washed with 5% trichloroacetic acid (TCA) solution, and then divided into two test tubes. One test tube contained Type I collagen One unit / ㎕ of type I collagenase was added, incubated at 37 ° C for 90 minutes, and the other tubes were stored at 4 ° C. Then, 0.05 ml of 50% trichloroacetic acid was added to all the tubes, and the mixture was allowed to stand at 4 ° C for 20 minutes, and then centrifuged at 12,000 rpm for 10 minutes at each time. The supernatant and the precipitate were separated by a liquid scintillation counter Counter), and the value of RCM (Relative Collagen Biosynthesis) was determined for the control group and the experimental group according to the following equation (1), and the results are shown in Table 4 below .

Common germinated sugar apple seed extract Subcritical Sugar Apple Seed Extract Subcritical sprouting sugar apple seed extract Common sugar apple seed extract Collagen Biosynthetic Rate (RCB) (%) 10 - - - 138 - 10 - - 138 - - 10 - 153 - - - 10 118 - - - - (Control group) 100

As shown in the results of Table 4, in the case of ordinary sugar apple seed extract, the ratio of collagen biosynthesis was about 118% as compared with the control sugar not containing sugar apple seed extract. However, in the case of germinated sugar apple extract and subcritical sugar apple seed extract, the ratio of collagen biosynthesis was 138% as compared with the control and that of the subcritical germinated sugar apple seed extract was 153% as the collagen biosynthesis ratio. Could know.

[Test Example 4] Sensory evaluation of lifting efficacy

Among the women aged 25 to 35, 50 persons suffering from skin sagging were divided into 5 groups of 10 persons each, and the lotion formulations of Formulation Examples 1 to 3 and Comparative Formulation Examples 1 and 2 were used for four weeks, , The degree of skin lifting efficacy according to the user's opinion was evaluated according to the following evaluation criteria. The results are shown in Table 5 below.

<Evaluation Criteria>

+++: Very good improvement.

++: Significant improvement.

+: There is a slight improvement.

±: No improvement, but no deterioration.

-: No improvement, but rather worse.

Test substance term 1 week 2 weeks 3 weeks 4 weeks Formulation Example 1 ++ ++ +++ +++ Formulation Example 2 ++ ++ +++ +++ Formulation Example 3 ++ +++ +++ +++ Comparative Formulation Example 1 + ++ ++ ++ Comparative Formulation Example 2 ± + + +

As can be seen in Table 5 above, Comparative Formulation Example 2, which does not contain sugar apple seed extract at all, Comparative Formulation Example 1, which contains common sugar apple seed extract, Formulation Example 2 containing the critical sugar apple seed extract and Formulation Example 3 containing the sub-critical germinated sugar apple seed extract showed rapid and excellent lifting efficacy.

Claims (14)

A cosmetic composition for anti-aging skin comprising an extract of germinated sugar apple seed as an active ingredient,
Wherein the skin aging is at least one selected from the group consisting of skin elasticity improvement, skin lifting enhancement, skin sagging improvement, and skin wrinkle improvement.
The cosmetic composition for skin antiaging according to claim 1, wherein the germinated sugar apple seed extract promotes collagen synthesis of the skin.
The cosmetic composition for skin antiaging according to claim 1, wherein the content of the germinated sugar apple seed extract is 0.01 to 95% by weight based on the total weight of the composition.
The germinated sugar apple seed according to claim 1, wherein the germinated sugar apple seed is dipped in water at a temperature of 21 to 27 캜 for 5 to 8 hours and then cultured at a temperature of 25 to 30 캜 for 16 to 18 hours And culturing for 5 to 7 hours under dark conditions, and germinating by repeating the treatment for 5 to 7 days.
[Claim 7] The cosmetic composition for skin antiaging according to claim 1, wherein the germinated sugar apple seed extract is obtained by extracting germinated sugar apple seed with an alcohol aqueous solution and then filtering it with a filter.
A cosmetic composition for skin aging comprising an extract of Sugar Apple Seed produced by subcritical extraction as an active ingredient,
Wherein the skin aging is at least one selected from the group consisting of skin elasticity improvement, skin lifting enhancement, skin sagging improvement, and skin wrinkle improvement.
[Claim 7] The cosmetic composition for skin aging according to claim 6, wherein the extract of Sugar Apple seed prepared by the subcritical extraction method promotes collagen synthesis of the skin.
[Claim 7] The cosmetic composition for skin aging according to claim 6, wherein the content of the sugar extract produced by the subcritical extraction method is 0.01 to 95% by weight based on the total weight of the composition.
[Claim 7] The method according to claim 6, wherein the extract of Sugar Apple seed prepared by the subcritical extraction method is obtained by extracting Sugar Apple seed at a high pressure of 50 to 200 MPa in a subcritical extraction apparatus, Cosmetic composition.
A cosmetic composition for anti-aging skin comprising an extract of germinated sugar apple seed prepared by subcritical extraction as an active ingredient,
Wherein the skin aging is at least one selected from the group consisting of skin elasticity improvement, skin lifting enhancement, skin sagging improvement, and skin wrinkle improvement.
[Claim 11] The cosmetic composition for skin aging according to claim 10, wherein the extract of germinated sugar apple seed prepared by the subcritical extraction method promotes collagen synthesis of the skin.
[Claim 11] The cosmetic composition for skin antiaging according to claim 10, wherein the content of germinated sugar apple seed extract prepared by the subcritical extraction method is 0.01 to 95% by weight based on the total weight of the composition.
The method according to claim 10, wherein the germinated sugar apple seed is obtained by dipping sugar apple seed in water at a temperature of 21 to 27 캜 for 5 to 8 hours and then culturing at 25 to 30 캜 for 16 to 18 hours And culturing for 5 to 7 hours under dark conditions, and germinating by repeating the treatment for 5 to 7 days.
[Claim 11] The method according to claim 10, wherein the germinated sugar apple seed extract prepared by the subcritical extraction method is obtained by extracting germinated sugar apple seeds at a high pressure of 50 to 200 MPa in a subcritical extraction apparatus, , A cosmetic composition for skin aging.