CN103705415A - Epidermal repair emulsion of meso-porous silicon carrying epidermal growth factor and preparation method thereof - Google Patents
Epidermal repair emulsion of meso-porous silicon carrying epidermal growth factor and preparation method thereof Download PDFInfo
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- CN103705415A CN103705415A CN201310692135.4A CN201310692135A CN103705415A CN 103705415 A CN103705415 A CN 103705415A CN 201310692135 A CN201310692135 A CN 201310692135A CN 103705415 A CN103705415 A CN 103705415A
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Abstract
The invention discloses an epidermal repair emulsion of meso-porous silicon carrying an epidermal growth factor and a preparation method thereof. The method comprises the following steps: (1) preparing a beauty emulsion matrix; (2) preparing meso-porous silicon carrying an epidermal growth factor; and (3) compounding to obtain the epidermal repair emulsion. The epidermal repair emulsion comprises a beauty emulsion matrix, meso-porous silicon carrying an epidermal growth factor and dimethicone at a mass ratio of (60-100): (1-5): (5-25). The epidermal repair emulsion of meso-porous silicon carrying an epidermal growth factor can promote cell metabolism and has the functions of moistening skin, enhancing skin elasticity, reducing skin wrinkles and delaying skin aging. After being coated with dimethicone, the meso-porous silicon carrying an epidermal growth factor can be added into any emulsion matrix, which is safe and stable and convenient and quick; the adhesiveness of the emulsion can be improved to realize good touch; and moreover, the biological activity of the epidermal growth factor protein can be maintained, and an aim of slowly releasing the epidermal growth factor can be achieved.
Description
Technical field
The present invention relates to technical field of skin care, particularly a kind of epidermis of the mesoporous silicon containing year epidermal growth factor is repaired breast and preparation method thereof.
Background technology
Along with growth in the living standard, people are more and more higher to the requirement of skin care item, and the skin care item of traditional general type more and more can not meet people's requirement, and biology gene engineering technology has brought brand-new development opportunity to cosmetics industry.Have in the market the skin care item containing epidermal growth factor, because its effect is well more a lot of than traditional skin care item composition, and natural having no side effect, so be deeply subject to liking of consumers in general.
Epidermal growth factor (Epidermal Growth Factor is called for short EGF) is formed and is comprised the short chain polypeptides of 3 disulfide bond by 53 amino acid residues; It has very strong biological effect, and the EGF of denier just can promote division and the growth of Skin Cell strongly, can also irritation cell the synthetic and secretion of outer macromole (as hyaluronic acid and glycoprotein etc.), there is the effect that promotes epidermal growth metabolism.Epidermal growth factor promotes body epidermis cell, epithelial cell, fibroblastic growth, division and metabolism in vivo, promotes microvascular growth, improves the microenvironment of Growth of Cells.In addition, epidermal growth factor can promote the synthetic of hydroxyproline, promotes the synthetic of collagen and collagenase, secretion Collagen material, hyaluronic acid and glycoprotein, regulate collagen fiber, there is skin care, strengthen skin elasticity, reduce wrinkle of skin and the effect that prevents skin aging.
But EGF is a kind of biological activity protein, very responsive to ambient temperature.In complicated cosmetic formulations, its biological activity is relatively hard to keep.And after EGF contact skin, can by the proteasome degradation of skin surface, be lost activity very soon.Yet EGF must have its effect of regular hour competence exertion, so must guarantee that EGF keeps its biological activity in skin care item, contact makes after skin EGF slowly discharge and can be fast by proteasome degradation, and its effect of guarantee is normally brought into play.
So far, epidermal growth factor has been reported in the application of skin care item industry.Chinese patent 201310287157.2 discloses a kind of vegetable oils facial treatment milk that contains activities of epidermal growth factor polypeptide, and activities of epidermal growth factor polypeptide is wrapped up by vegetable oil, protects its activity.Chinese patent 201110362124.0 discloses and has a kind ofly contributed to keep the bioactive cosmetic composition of epiderm skin somatomedin, thereby utilize, various constituent structure are similar with EGF to be protected it.Chinese patent 200810050569.3 discloses human growth hormone and the application of human epidermal growth factor combination in beauty treatment, and their applied in any combination is in beauty treatment, mutually collaborative.They still make us relieved not to the protection of activities of epidermal growth factor, and after contact skin, can not reach the effect of slow release.
Summary of the invention
The object of the invention is to for the deficiencies in the prior art; provide mesoporous silicon in emulsion, to bring into play the new application of protection EGF effect; the epidermis that is a kind of mesoporous silicon containing year epidermal growth factor is repaired breast and preparation method thereof, and epidermal growth factor is written in hexagonal mesoporous silicon, by dimethicone, wraps up; be positioned in oil phase; then add in beauty treatment emulsion matrix, by homogenizing effect, it is evenly distributed in emulsion; by the Transdermal absorption of oil phase, it is more easily played a role.Both the biological activity of egf protein can be kept, and the object of slow release EGF can be reached again.
Containing the epidermis that carries the mesoporous silicon of epidermal growth factor, repair a newborn preparation method, comprise the following steps:
(1) preparation beauty treatment emulsion matrix:
1) Rosehips oil, almond oil, liquid paraffin, spermaceti, spermol, glyceryl monostearate being mixed, be heated to 75 ~ 85 ℃, is under 200 ~ 300rpm condition, to carry out magnetic agitation dissolving at rotating speed, obtains A phase;
2) by glycerol, triethanolamine, at rotating speed, be under 150 ~ 300rpm condition, to carry out magnetic agitation mixing, be heated to 75 ~ 85 ℃, obtain B phase;
3) B is added to A mutually in, at rotating speed, be under 150 ~ 300rpm condition, to carry out magnetic agitation, be warming up to 90 ~ 95 ℃ and keep 8 ~ 10min to be cooled to again 60 ~ 65 ℃, then add deionized water, mix, with high-shear homogenizer homogenizing 10 ~ 15min under 5000 ~ 8000rpm speed;
4) remain on 60 ~ 65 ℃, under 300 ~ 500rpm condition, with dasher, stir 30 ~ 40min, fully emulsified after, continue to stir until bubble is all driven away, then add antiseptic, spice, be cooled to room temperature and obtain the emulsion matrix of improving looks;
(2) carry the preparation of the mesoporous silicon of epidermal growth factor:
Take in the phosphate-buffered liquor that epidermal growth factor is dissolved in 0.02M and be placed in seal glass tank, add hexagonal mesoporous silicon, put into 36 ~ 38 ℃ of shaking tables, centrifugal through deionized water wash 3 times, lyophilization 24 ~ 30h in freezer dryer, the mesoporous silicon that obtains carrying epidermal growth factor, is put in-4 ℃ of refrigerators standby;
(3) carry epidermal growth factor mesoporous silicon and beauty treatment emulsion matrix composite:
1) at room temperature by carrying mesoporous silicon and the dimethicone agate mortar mix homogeneously of epidermal growth factor, form after homogeneous mixture, add in beauty treatment emulsion matrix;
2) at rotating speed, be with dasher, constantly to stir and be cooled to room temperature under 300 ~ 500rpm condition, obtain repairing breast containing the epidermis that carries the mesoporous silicon of epidermal growth factor.
In above-mentioned preparation method, described beauty treatment emulsion matrix comprises the emulsion matrix that following quality percentage composition material forms: Rosehips oil 8% ~ 16%, almond oil 10% ~ 16%, liquid paraffin 6% ~ 8%, spermaceti 4% ~ 6%, spermol 2% ~ 4%, glyceryl monostearate 2% ~ 4%, glycerol 6% ~ 8%, triethanolamine 4% ~ 8%, antiseptic 0.3% ~ 0.5%, spice 0.5% ~ 0.7%, deionized water 29% ~ 57%.
In above-mentioned preparation method, described in step (2), the concentration of epidermal growth factor in phosphate-buffered liquor is 3 ~ 5mg/L; The mass ratio of described epidermal growth factor and mesoporous silicon is 1:30 ~ 50.
The epidermis reparation breast of above-mentioned preparation method gained comprises beauty treatment emulsion matrix, carries mesoporous silicon and the dimethicone of epidermal growth factor, and the emulsion matrix of wherein improving looks, year mesoporous silicon of epidermal growth factor are, the mass ratio of dimethicone is 60 ~ 100:1 ~ 5:5 ~ 25.
Compared with prior art, the invention has the advantages that:
(1) containing the epidermis reparation breast that carries the mesoporous silicon of epidermal growth factor, can promote cell metabolism, there is skin care, strengthen skin elasticity, reduce the effect of wrinkle of skin and delay skin aging.
(2) after being wrapped up by dimethicone, can add to the mesoporous silicon that carries epidermal growth factor arbitrarily in emulsion matrix, safety and stability, and convenient and swift, can improve the cohesiveness of emulsion, make sense of touch good.
(3) epidermal growth factor is written in hexagonal mesoporous silicon, can keep its biological activity, improves its stability.And due to the special pore passage structure of mesoporous silicon, reach the effect to epidermal growth factor slow release.
accompanying drawing explanation:
Fig. 1 does not repair the release in vitro figure of products obtained therefrom in breast and embodiment 1 containing the epidermis of mesoporous silicon.
The specific embodiment
Following examples will further illustrate the preferred embodiment belonging in the scope of the invention.These embodiment are only for illustrational object, but protection scope of the present invention is not limited to this, because the present invention can have many change programmes under the prerequisite that does not deviate from essence of the present invention or scope.
case study on implementation 1:
(1) preparation beauty treatment emulsion matrix:
1) 8g Rosehips oil, 10g almond oil, 6g liquid paraffin, 4g spermaceti, 2g spermol, 2g glyceryl monostearate being mixed, be heated to 75 ℃, is under 200rpm condition, to carry out magnetic agitation dissolving at rotating speed, obtains A phase;
2) by 6g glycerol, 4g triethanolamine, at rotating speed, be under 150rpm condition, to carry out magnetic agitation mixing, be heated to 75 ℃, obtain B phase;
3) B is added to A mutually in, at rotating speed, be under 150rpm condition, to carry out magnetic agitation, be warming up to 90 ℃ and keep 8min to be cooled to again 60 ℃, then add 57g deionized water, mix, with high-shear homogenizer homogenizing 10min under 5000rpm speed;
4) remain on 60 ℃, under 300rpm condition, with dasher, stir 30min, fully emulsified after, continue to stir until bubble is all driven away, then add 0.3g antiseptic, 0.7g spice, be cooled to room temperature and obtain the emulsion matrix of improving looks;
(2) carry the preparation of the mesoporous silicon of epidermal growth factor:
Take in the phosphate-buffered liquor of 0.02M that 0.3mg epidermal growth factor is dissolved in 100mL and be placed in seal glass tank, add the hexagonal mesoporous silicon of 15mg, put into 36 ℃ of shaking tables, centrifugal through deionized water wash 3 times, lyophilization 24h in freezer dryer, obtains product and is put in-4 ℃ of refrigerators standby;
(3) carry epidermal growth factor mesoporous silicon and beauty treatment emulsion matrix composite:
1) at room temperature 0.1g is carried to mesoporous silicon and the 0.5g dimethicone agate mortar mix homogeneously of epidermal growth factor, form after homogeneous mixture, add in 6g beauty treatment emulsion matrix;
2) at rotating speed, be with dasher, constantly to stir and be cooled to room temperature under 300rpm condition, obtain repairing breast containing the epidermis that carries the mesoporous silicon of epidermal growth factor.
Repair the mensuration of newborn release in vitro: the sample that precision measures 500 μ L is placed in bag filter, bag filter is put into the beaker of 500mL, add 500mL's, heating in water bath maintains the temperature at 37 ± 1 ℃, the mixing speed of 200 rpm, respectively at 0.5h, 1h, 2h, 3h, 4h, 5h, 6h, 7h, 8h, 9h, 10h, 11h, 12h, 24h takes out 5mL acceptable solution, the blank 50% dehydrated alcohol-PBS buffer that simultaneously adds 5mL, utilize the epidermal growth factor concentration in determined by ultraviolet spectrophotometry dialysis solution, total content * 100% of amount/reparation Ruzhong epidermal growth factor of release=release medium Concentrations of Epidermal Growth Factor concentration.Release total time is 24h, obtains drug release curve according to time and cumulative release percentage ratio.
Containing the epidermis reparation breast that carries the mesoporous silicon of epidermal growth factor, repair newborn release in vitro result as shown in Figure 1 with the epidermal growth factor that does not contain mesoporous silicon, can find out very not fast containing reparation breast rate of release before 3h of mesoporous silicon, during 3h, just discharged 50.8%, discharge afterwards slowly, burst size is less, prominent releasing acts on by force, without slow release effect.And very fast containing reparation breast drug release rate before 10h of mesoporous silicon, burst size accounts for 49.1 % of total content.After 24h, release amount accounts for 52.4% of total dose.Without obvious burst effect, epidermal growth factor shows good sustained release performance.
case study on implementation 2:
(1) preparation beauty treatment emulsion matrix:
1) 12g Rosehips oil, 13g almond oil, 7g liquid paraffin, 5g spermaceti, 3g spermol, 3g glyceryl monostearate being mixed, be heated to 80 ℃, is under 250rpm condition, to carry out magnetic agitation dissolving at rotating speed, obtains A phase;
2) by 7g glycerol, 6g triethanolamine, at rotating speed, be under 200rpm condition, to carry out magnetic agitation mixing, be heated to 80 ℃, obtain B phase;
3) B is added to A mutually in, at rotating speed, be under 200rpm condition, to carry out magnetic agitation, be warming up to 90 ℃ and keep 9min to be cooled to again 60 ℃, then add 43g deionized water, mix, with high-shear homogenizer homogenizing 15min under 6500rpm speed;
4) remain on 60 ℃, under 400rpm condition, with dasher, stir 35min, fully emulsified after, continue to stir until bubble is all driven away, then add 0.4g antiseptic, 0.6g spice, be cooled to room temperature and obtain the emulsion matrix of improving looks;
(2) carry the preparation of the mesoporous silicon of epidermal growth factor:
Take in the phosphate-buffered liquor of 0.02M that 0.4mg epidermal growth factor is dissolved in 100mL and be placed in seal glass tank, add the hexagonal mesoporous silicon of 16mg, put into 37 ℃ of shaking tables, centrifugal through deionized water wash 3 times, lyophilization 27h in freezer dryer, obtains product and is put in-4 ℃ of refrigerators standby;
(3) carry epidermal growth factor mesoporous silicon and beauty treatment emulsion matrix composite:
1) at room temperature 0.3g is carried to mesoporous silicon and the 1.5g dimethicone agate mortar mix homogeneously of epidermal growth factor, form after homogeneous mixture, add in 8g beauty treatment emulsion matrix;
2) at rotating speed, be with dasher, constantly to stir and be cooled to room temperature under 400rpm condition, obtain repairing breast containing the epidermis that carries the mesoporous silicon of epidermal growth factor.
The epidermis reparation breast containing carrying the mesoporous silicon of epidermal growth factor that this embodiment is made carries out epidermal growth factor and discharges test, and before 10h, drug release rate is very fast, and burst size accounts for 51.3 % of total content.After 24h, release amount accounts for 54.8% of total dose.Without obvious burst effect, epidermal growth factor shows good sustained release performance.
case study on implementation 3:
(1) preparation beauty treatment emulsion matrix:
1) 16g Rosehips oil, 16g almond oil, 8g liquid paraffin, 6g spermaceti, 4g spermol, 4g glyceryl monostearate being mixed, be heated to 85 ℃, is under 300rpm condition, to carry out magnetic agitation dissolving at rotating speed, obtains A phase;
2) by 8g glycerol, 8g triethanolamine, at rotating speed, be under 300rpm condition, to carry out magnetic agitation mixing, be heated to 85 ℃, obtain B phase;
3) B is added to A mutually in, at rotating speed, be under 300rpm condition, to carry out magnetic agitation, be warming up to 95 ℃ and keep 10min to be cooled to again 65 ℃, then add 29g deionized water, mix, with high-shear homogenizer homogenizing 15min under 8000rpm speed;
4) remain on 65 ℃, under 500rpm condition, with dasher, stir 40min, fully emulsified after, continue to stir until bubble is all driven away, then add 0.5g antiseptic, 0.5g spice, be cooled to room temperature and obtain the emulsion matrix of improving looks;
(2) carry the preparation of the mesoporous silicon of epidermal growth factor:
Take in the phosphate-buffered liquor of 0.02M that 0.5mg epidermal growth factor is dissolved in 100mL and be placed in seal glass tank, add the hexagonal mesoporous silicon of 15mg, put into 38 ℃ of shaking tables, centrifugal through deionized water wash 3 times, lyophilization 30h in freezer dryer, obtains product and is put in-4 ℃ of refrigerators standby;
(3) carry epidermal growth factor mesoporous silicon and beauty treatment emulsion matrix composite:
1) at room temperature 0.5g is carried to mesoporous silicon and the 2.5g dimethicone agate mortar mix homogeneously of epidermal growth factor, form after homogeneous mixture, add in 10g beauty treatment emulsion matrix;
2) at rotating speed, be with dasher, constantly to stir and be cooled to room temperature under 500rpm condition, obtain repairing breast containing the epidermis that carries the mesoporous silicon of epidermal growth factor.
The epidermis reparation breast containing carrying the mesoporous silicon of epidermal growth factor that this embodiment is made carries out epidermal growth factor and discharges test, and before 10h, drug release rate is very fast, and burst size accounts for 54.7 % of total content.After 24h, release amount accounts for 57.3% of total dose.Without obvious burst effect, epidermal growth factor shows good sustained release performance.
case study on implementation 4:
The epidermis reparation breast containing carrying the mesoporous silicon of epidermal growth factor of case being implemented to 1,2,3 preparations carries out stability experiment, and experimental technique is as follows:
1, centrifugal stability: reparation breast is put into the centrifugal 30min with centrifuge 4000r/min, and reparation breast is stablized not stratified, keeps clear, illustrates and repairs newborn centrifugal good stability.
2, low-temperature stability: reparation breast is placed 30 days in 4 ℃ of refrigerators, recovered to observe after room temperature, reparation breast is stablized not stratified, illustrates that the newborn low-temperature stability of reparation is good.
3, temperature cycles stability: reparation breast is placed to 24h in 4 ℃ of refrigerators, then place 24h in 40 ℃ of baking ovens, circulate 30 days, reparation breast is stablized not stratified, illustrates and repairs newborn centrifugal good stability.
4, accelerated stability: place 3 months for 40 ℃, respectively at 1,2, observe March, repair breast and stablize not stratifiedly, illustrate that the newborn accelerated stability of reparation is good.
5, long-time stability: be sealed in finger bottle, room temperature is placed 6 months, respectively at 1,2,3,4,5, observe June, repair breast and stablize not stratifiedly, illustrates that the newborn long-time stability of reparation are good.
Experimental result sees the following form:
| Stability | Phenomenon | Conclusion |
| Centrifugal stability | Keep clear, stablize not stratified | Centrifugal good stability |
| Low-temperature stability | Keep clear, stablize not stratified | Low-temperature stability is good |
| Temperature cycles stability | Keep clear, stablize not stratified | Temperature cycles good stability |
| Accelerated stability | Keep clear, stablize not stratified | Accelerated stability is good |
| Long-time stability | Keep clear, stablize not stratified | Long-time stability are good |
case study on implementation 5:
To executing the epidermis of the prepared a kind of mesoporous silicon containing year epidermal growth factor of case 1, repair newborn measure of merit:
For understanding the result of use of product of the present invention, with the form of questionnaire survey, respectively the effect of wrinkle removing, skin care, anti-acne is launched to investigation on probation widely.
Select trier 50 people to investigate the effect of its wrinkle removing, wherein men and women half and half, at 40 ~ 50 years old age, regains altogether 50 parts of questionnaires.After clean of every day in morning, be applied to face and exposed part, once a day, adhere to 60 days.
Select trier 50 people to investigate the effect of its skin care, wherein men and women half and half, at 30 ~ 48 years old age, regains altogether 50 parts of questionnaires.After clean of every day in morning, be applied to face and exposed part, once a day, adhere to 60 days.
Select trier 100 people to investigate the effect of its anti-acne, wherein men and women half and half, at 16 ~ 25 years old age, regains altogether 98 parts of questionnaires.After clean of every day in morning, be applied to face and exposed part, once a day, adhere to 60 days.
According to trier's range estimation and sense learning through practice, indices and result of use self evaluation result are as follows:
1. wrinkle removing effect: 68% trier has obvious wrinkle to alleviate light effect, and 20% trier has slight wrinkle to subtract light use sense, and 12% trier is without obvious sensation.
2. skin care effect: 72% trier has obvious sensation skin to become careful soft, 22% trier feels that skin has and becomes a little careful soft, 6% trier is without obvious sensation.
3. anti-acne effect: 53% trier has the disappear effect of pox of obvious anti-acne, 35% the trier small pox symptom of feeling has and alleviates or the effect of slight anti-acne, and 12% trier is without obvious sensation.
4. safety: 100% trier is without anaphylaxis.
Same, for case study on implementation 2,3, done corresponding investigation on probation simultaneously, the effect evaluation result of survey result and case study on implementation 1 is substantially without significant difference, can find out containing the epidermis that carries the mesoporous silicon of epidermal growth factor and repair breast to skin nonirritant, and use comfortable, and have the good crease-resistant effect of anti-acne skin care, reach the effect of looks improving and the skin nourishing.
Claims (4)
1. containing the epidermis that carries the mesoporous silicon of epidermal growth factor, repair a newborn preparation method, it is characterized in that, comprise the following steps:
(1) preparation beauty treatment emulsion matrix:
1) Rosehips oil, almond oil, liquid paraffin, spermaceti, spermol, glyceryl monostearate being mixed, be heated to 75 ~ 85 ℃, is under 200 ~ 300rpm condition, to carry out magnetic agitation dissolving at rotating speed, obtains A phase;
2) by glycerol, triethanolamine, at rotating speed, be under 150 ~ 300rpm condition, to carry out magnetic agitation mixing, be heated to 75 ~ 85 ℃, obtain B phase;
3) B is added to A mutually in, at rotating speed, be under 150 ~ 300rpm condition, to carry out magnetic agitation, be warming up to 90 ~ 95 ℃ and keep 8 ~ 10min to be cooled to again 60 ~ 65 ℃, then add deionized water, mix, with high-shear homogenizer homogenizing 10 ~ 15min under 5000 ~ 8000rpm speed;
4) remain on 60 ~ 65 ℃, under 300 ~ 500rpm condition, with dasher, stir 30 ~ 40min, fully emulsified after, continue to stir until bubble is all driven away, then add antiseptic, spice, be cooled to room temperature and obtain the emulsion matrix of improving looks;
(2) carry the preparation of the mesoporous silicon of epidermal growth factor:
Take in the phosphate-buffered liquor that epidermal growth factor is dissolved in 0.02M and be placed in seal glass tank, add hexagonal mesoporous silicon, put into 36 ~ 38 ℃ of shaking tables, centrifugal through deionized water wash 3 times, lyophilization 24 ~ 30h in freezer dryer, the mesoporous silicon that obtains carrying epidermal growth factor, is put in-4 ℃ of refrigerators standby;
(3) carry epidermal growth factor mesoporous silicon and beauty treatment emulsion matrix composite:
1) at room temperature by carrying mesoporous silicon and the dimethicone agate mortar mix homogeneously of epidermal growth factor, form after homogeneous mixture, add in beauty treatment emulsion matrix;
2) at rotating speed, be with dasher, constantly to stir and be cooled to room temperature under 300 ~ 500rpm condition, obtain repairing breast containing the epidermis that carries the mesoporous silicon of epidermal growth factor.
2. the epidermis of a kind of mesoporous silicon containing year epidermal growth factor according to claim 1 is repaired newborn preparation method, it is characterized in that, described beauty treatment emulsion matrix comprises the emulsion matrix that following quality percentage composition material forms: Rosehips oil 8% ~ 16%, almond oil 10% ~ 16%, liquid paraffin 6% ~ 8%, spermaceti 4% ~ 6%, spermol 2% ~ 4%, glyceryl monostearate 2% ~ 4%, glycerol 6% ~ 8%, triethanolamine 4% ~ 8%, antiseptic 0.3% ~ 0.5%, spice 0.5% ~ 0.7%, deionized water 29% ~ 57%.
3. the epidermis of a kind of mesoporous silicon containing year epidermal growth factor according to claim 1 is repaired newborn preparation method, it is characterized in that, described in step (2), the concentration of epidermal growth factor in phosphate-buffered liquor is 3 ~ 5mg/L; The mass ratio of described epidermal growth factor and mesoporous silicon is 1:30 ~ 50.
4. a kind of epidermis reparation breast containing carrying the mesoporous silicon of epidermal growth factor being prepared by preparation method described in claim 1-3, it is characterized in that, described epidermis reparation breast comprises beauty treatment emulsion matrix, carries mesoporous silicon and the dimethicone of epidermal growth factor, and the emulsion matrix of wherein improving looks, year mesoporous silicon of epidermal growth factor are, the mass ratio of dimethicone is 60 ~ 100:1 ~ 5:5 ~ 25.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104225600A (en) * | 2014-09-11 | 2014-12-24 | 华南理工大学 | Preparation method of wound antibacterial emulsion containing hexagonal mesoporous silica |
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| CN102380102A (en) * | 2011-11-02 | 2012-03-21 | 东华大学 | Method for preparing environment-responsive mesoporous silicon nanoparticles |
| CN102406556A (en) * | 2011-11-16 | 2012-04-11 | 无限极（中国）有限公司 | Cosmetic composition favorable for keeping biological activity of epidermal growth factor (EGF) |
| CN103271837A (en) * | 2013-06-07 | 2013-09-04 | 王迎峰 | Formula and preparation method for bioactive cosmetic |
| CN103343137A (en) * | 2013-07-10 | 2013-10-09 | 吉林农业大学 | Vegetable oil body skin-care emulsion containing epidermal growth factor active polypeptide |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102380102A (en) * | 2011-11-02 | 2012-03-21 | 东华大学 | Method for preparing environment-responsive mesoporous silicon nanoparticles |
| CN102406556A (en) * | 2011-11-16 | 2012-04-11 | 无限极（中国）有限公司 | Cosmetic composition favorable for keeping biological activity of epidermal growth factor (EGF) |
| CN103271837A (en) * | 2013-06-07 | 2013-09-04 | 王迎峰 | Formula and preparation method for bioactive cosmetic |
| CN103343137A (en) * | 2013-07-10 | 2013-10-09 | 吉林农业大学 | Vegetable oil body skin-care emulsion containing epidermal growth factor active polypeptide |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104225600A (en) * | 2014-09-11 | 2014-12-24 | 华南理工大学 | Preparation method of wound antibacterial emulsion containing hexagonal mesoporous silica |
| CN104225600B (en) * | 2014-09-11 | 2016-09-21 | 华南理工大学 | A kind of preparation method of the Wound antibiotic breast containing hexagonal mesoporous silicon |
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