CN103705415B - A kind of epidermis of the mesoporous silicon containing carrying epidermal growth factor repairs breast and preparation method thereof - Google Patents
A kind of epidermis of the mesoporous silicon containing carrying epidermal growth factor repairs breast and preparation method thereof Download PDFInfo
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Abstract
The epidermis that the invention discloses a kind of mesoporous silicon containing carrying epidermal growth factor repairs breast and preparation method thereof.Its method is as follows: (1) preparation beauty treatment emulsion matrix; (2) preparation of the mesoporous silicon of epidermal growth factor is carried; (3) composite, obtain epidermis and repair breast.Described epidermis reparation breast comprises beauty treatment emulsion matrix, the mesoporous silicon carrying epidermal growth factor and dimethicone, and its mass ratio is 60 ~ 100:1 ~ 5:5 ~ 25.Epidermis reparation breast containing the mesoporous silicon carrying epidermal growth factor can promote cell metabolism, have skin care, strengthens skin elasticity, reduces wrinkle of skin and delay skin aging.And carry after the mesoporous silicon of epidermal growth factor is wrapped up by dimethicone and can add in arbitrary emulsion matrix, safety and stability, convenient and swift, the cohesiveness of emulsion can be improved, make sense of touch good, both can keep the biological activity of egf protein, the object of slow release epidermal growth factor can have been reached again.
Description
Technical field
The present invention relates to technical field of skin care, particularly a kind of epidermis of the mesoporous silicon containing carrying epidermal growth factor repairs breast and preparation method thereof.
Background technology
Along with growth in the living standard, the requirement of people to skin care item is more and more higher, and the skin care item of traditional general type more and more can not meet require that of people, and biology gene engineering technology brings brand-new development opportunity to cosmetics industry.The existing skin care item containing epidermal growth factor in the market, because its effect is well more a lot of than traditional skin care item composition, and naturally to have no side effect, so dark liking by consumers in general.
Epidermal growth factor (Epidermal Growth Factor is called for short EGF) is made up of 53 amino acid residues and comprises the short chain polypeptides of 3 disulfide bond; It has very strong biological effect, and the EGF of denier just can promote division and the growth of Skin Cell strongly, can also the synthesis of the outer macromole (as hyaluronic acid and glycoprotein etc.) of irritation cell and secretion, has the effect promoting epidermal growth metabolism.Epidermal growth factor promotes body epidermis cell, epithelial cell, fibroblastic growth, division and metabolism in vivo, promotes microvascular growth, improves the microenvironment of Growth of Cells.In addition, epidermal growth factor can promote the synthesis of hydroxyproline, promotes the synthesis of collagen and collagenase, secretion Collagen material, hyaluronic acid and glycoprotein, regulate collagen fiber, there is skin care, strengthen skin elasticity, reduce wrinkle of skin and the effect preventing skin aging.
But EGF is a kind of biological activity protein, very responsive to ambient temperature.In the cosmetic formulations of complexity, its biological activity compares and is hard to keep.And after EGF contacts skin, can be lost activity by the proteasome degradation of skin surface very soon.But EGF must have its effect of regular hour competence exertion, so must guarantee that EGF keeps its biological activity in skin care item, make EGF slow releasing after contact skin and can not be easily degraded by proteases fast, its effect of guarantee normally plays.
So far, epidermal growth factor has been reported in the application of skin care item industry.Chinese patent 201310287157.2 discloses a kind of vegetable oils facial treatment milk containing activities of epidermal growth factor polypeptide, is wrapped up by activities of epidermal growth factor polypeptide by vegetable oil, protects it active.Chinese patent 201110362124.0 discloses one and contributes to keeping the bioactive cosmetic composition of epiderm skin somatomedin, utilizes various constituent structure similar with EGF thus protects it.Chinese patent 200810050569.3 discloses human growth hormone and the application of human epidermal growth factor combination in beauty treatment, by their Combination application in beauty treatment, mutually works in coordination with.They still make us relieved not to the protection of activities of epidermal growth factor, and can not reach the effect of slow releasing after contact skin.
Summary of the invention
The object of the invention is to for the deficiencies in the prior art; there is provided mesoporous silicon in emulsion, play the new application of protection EGF effect; namely a kind of epidermis of the mesoporous silicon containing carrying epidermal growth factor repairs breast and preparation method thereof, is loaded in hexagonal mesoporous silicon by epidermal growth factor, is wrapped up by dimethicone; be positioned in oil phase; then add in beauty treatment emulsion matrix, by homogenizing effect, make it be evenly distributed in emulsion; by the Transdermal absorption of oil phase, it is made more easily easily to play a role.Both can keep the biological activity of egf protein, the object of slow release EGF can have been reached again.
Epidermis containing the mesoporous silicon carrying epidermal growth factor repairs a preparation method for breast, comprises the following steps:
(1) preparation beauty treatment emulsion matrix:
1) by Rosehips oil, almond oil, liquid paraffin, spermaceti, spermol, glyceryl monostearate mixing, be heated to 75 ~ 85 DEG C, under rotating speed is 200 ~ 300rpm condition, carries out magnetic agitation dissolving, obtain A phase;
2) glycerol, triethanolamine are carried out magnetic agitation mixing under rotating speed is 150 ~ 300rpm condition, be heated to 75 ~ 85 DEG C, obtain B phase;
3) B is added in A phase, under rotating speed is 150 ~ 300rpm condition, carries out magnetic agitation, is warming up to 90 ~ 95 DEG C and keeps 8 ~ 10min to be cooled to 60 ~ 65 DEG C again, then add deionized water, mixing, with high-shear homogenizer homogenizing 10 ~ 15min under 5000 ~ 8000rpm speed;
4) remain on 60 ~ 65 DEG C, under 300 ~ 500rpm condition, stir 30 ~ 40min with dasher, after fully emulsified, continue to stir until bubble is all driven away, then add antiseptic, spice, be cooled to room temperature and obtain emulsion matrix of improving looks;
(2) preparation of the mesoporous silicon of epidermal growth factor is carried:
Take epidermal growth factor to be dissolved in the phosphate buffer of 0.02M and to be placed in seal glass tank, add hexagonal mesoporous silicon, put into 36 ~ 38 DEG C of shaking tables, centrifugal through deionized water wash 3 times, lyophilization 24 ~ 30h in freezer dryer, obtain the mesoporous silicon carrying epidermal growth factor, be put in-4 DEG C of refrigerators for subsequent use;
(3) mesoporous silicon and the beauty treatment emulsion matrix that carry epidermal growth factor are composite:
1) at room temperature will carry mesoporous silicon and the dimethicone agate mortar mix homogeneously of epidermal growth factor, after forming homogeneous mixture, add in beauty treatment emulsion matrix;
2) constantly stir with dasher under rotating speed is 300 ~ 500rpm condition and be cooled to room temperature, the epidermis obtained containing the mesoporous silicon carrying epidermal growth factor repairs breast.
In above-mentioned preparation method, described beauty treatment emulsion matrix comprises the emulsion matrix of following mass percentage material composition: Rosehips oil 8% ~ 16%, almond oil 10% ~ 16%, liquid paraffin 6% ~ 8%, spermaceti 4% ~ 6%, spermol 2% ~ 4%, glyceryl monostearate 2% ~ 4%, glycerol 6% ~ 8%, triethanolamine 4% ~ 8%, antiseptic 0.3% ~ 0.5%, spice 0.5% ~ 0.7%, deionized water 29% ~ 57%.
In above-mentioned preparation method, described in step (2), the concentration of epidermal growth factor in phosphate buffer is 3 ~ 5mg/L; The mass ratio of described epidermal growth factor and mesoporous silicon is 1:30 ~ 50.
The epidermis reparation breast of above-mentioned preparation method gained comprises beauty treatment emulsion matrix, the mesoporous silicon carrying epidermal growth factor and dimethicone, and emulsion matrix of wherein improving looks, year mesoporous silicon of epidermal growth factor, the mass ratio of dimethicone are 60 ~ 100:1 ~ 5:5 ~ 25.
Compared with prior art, the invention has the advantages that:
(1) the epidermis reparation breast containing the mesoporous silicon carrying epidermal growth factor can promote cell metabolism, have skin care, strengthens skin elasticity, reduces the effect of wrinkle of skin and delay skin aging.
(2) can add in arbitrary emulsion matrix after the mesoporous silicon carrying epidermal growth factor is wrapped up by dimethicone, safety and stability, convenient and swift, the cohesiveness of emulsion can be improved, make sense of touch good.
(3) epidermal growth factor is loaded in hexagonal mesoporous silicon, can keep its biological activity, improves its stability.And due to the special pore passage structure of mesoporous silicon, reach the effect to epidermal growth factor slow release.
accompanying drawing illustrates:
Fig. 1 does not repair release in vitro figure that is newborn and products obtained therefrom in embodiment 1 containing the epidermis of mesoporous silicon.
Detailed description of the invention
Following examples will illustrate the preferred embodiment belonged in the scope of the invention further.These embodiments are only for illustrational object, but protection scope of the present invention is not limited to this, because the present invention can have many change programmes under the prerequisite not deviating from essence of the present invention or scope.
case study on implementation 1:
(1) preparation beauty treatment emulsion matrix:
1) by 8g Rosehips oil, 10g almond oil, 6g liquid paraffin, 4g spermaceti, 2g spermol, the mixing of 2g glyceryl monostearate, be heated to 75 DEG C, under rotating speed is 200rpm condition, carries out magnetic agitation dissolving, obtain A phase;
2) 6g glycerol, 4g triethanolamine are carried out magnetic agitation mixing under rotating speed is 150rpm condition, be heated to 75 DEG C, obtain B phase;
3) be added to by B in A phase, under rotating speed is 150rpm condition, carries out magnetic agitation, be warming up to 90 DEG C and keep 8min to be cooled to 60 DEG C again, then add 57g deionized water, mixing, with high-shear homogenizer homogenizing 10min under 5000rpm speed;
4) remain on 60 DEG C, under 300rpm condition, stir 30min with dasher, after fully emulsified, continue to stir until bubble is all driven away, then add 0.3g antiseptic, 0.7g spice, be cooled to room temperature and obtain emulsion matrix of improving looks;
(2) preparation of the mesoporous silicon of epidermal growth factor is carried:
Take 0.3mg epidermal growth factor to be dissolved in the phosphate buffer of the 0.02M of 100mL and to be placed in seal glass tank, add the hexagonal mesoporous silicon of 15mg, put into 36 DEG C of shaking tables, centrifugal through deionized water wash 3 times, lyophilization 24h in freezer dryer, obtains product and is put in-4 DEG C of refrigerators for subsequent use;
(3) mesoporous silicon and the beauty treatment emulsion matrix that carry epidermal growth factor are composite:
1) at room temperature 0.1g is carried mesoporous silicon and the 0.5g dimethicone agate mortar mix homogeneously of epidermal growth factor, after forming homogeneous mixture, add 6g to and improve looks in emulsion matrix;
2) constantly stir with dasher under rotating speed is 300rpm condition and be cooled to room temperature, the epidermis obtained containing the mesoporous silicon carrying epidermal growth factor repairs breast.
Repair the mensuration of newborn release in vitro: the sample that precision measures 500 μ L is placed in bag filter, bag filter is put into the beaker of 500mL, add 500mL's, heating in water bath maintains the temperature at 37 ± 1 DEG C, the mixing speed of 200 rpm, respectively at 0.5h, 1h, 2h, 3h, 4h, 5h, 6h, 7h, 8h, 9h, 10h, 11h, 12h, 24h takes out 5mL acceptable solution, add the blank 50% dehydrated alcohol-PBS buffer of 5mL simultaneously, utilize the epidermal growth factor concentration in determined by ultraviolet spectrophotometry dialysis solution, total content × 100% of the amount/reparation Ruzhong epidermal growth factor of release=release medium Concentrations of Epidermal Growth Factor concentration.Release total time is 24h, obtains drug release patterns according to time and cumulative release percentage ratio.
Epidermis containing the mesoporous silicon carrying epidermal growth factor is repaired breast and is not repaired the release in vitro result of breast as shown in Figure 1 containing the epidermal growth factor of mesoporous silicon, can find out that the reparation breast rate of release before 3h not containing mesoporous silicon is very fast, just 50.8% is released during 3h, release afterwards slowly, burst size is less, prominent releasing acts on by force, without slow release effect.And very fast containing reparation breast drug release rate before 10h of mesoporous silicon, burst size accounts for 49.1 % of total content.After 24h, release amount accounts for 52.4% of total dose.Without obvious burst effect, epidermal growth factor shows good sustained release performance.
case study on implementation 2:
(1) preparation beauty treatment emulsion matrix:
1) by 12g Rosehips oil, 13g almond oil, 7g liquid paraffin, 5g spermaceti, 3g spermol, the mixing of 3g glyceryl monostearate, be heated to 80 DEG C, under rotating speed is 250rpm condition, carries out magnetic agitation dissolving, obtain A phase;
2) 7g glycerol, 6g triethanolamine are carried out magnetic agitation mixing under rotating speed is 200rpm condition, be heated to 80 DEG C, obtain B phase;
3) be added to by B in A phase, under rotating speed is 200rpm condition, carries out magnetic agitation, be warming up to 90 DEG C and keep 9min to be cooled to 60 DEG C again, then add 43g deionized water, mixing, with high-shear homogenizer homogenizing 15min under 6500rpm speed;
4) remain on 60 DEG C, under 400rpm condition, stir 35min with dasher, after fully emulsified, continue to stir until bubble is all driven away, then add 0.4g antiseptic, 0.6g spice, be cooled to room temperature and obtain emulsion matrix of improving looks;
(2) preparation of the mesoporous silicon of epidermal growth factor is carried:
Take 0.4mg epidermal growth factor to be dissolved in the phosphate buffer of the 0.02M of 100mL and to be placed in seal glass tank, add the hexagonal mesoporous silicon of 16mg, put into 37 DEG C of shaking tables, centrifugal through deionized water wash 3 times, lyophilization 27h in freezer dryer, obtains product and is put in-4 DEG C of refrigerators for subsequent use;
(3) mesoporous silicon and the beauty treatment emulsion matrix that carry epidermal growth factor are composite:
1) at room temperature 0.3g is carried mesoporous silicon and the 1.5g dimethicone agate mortar mix homogeneously of epidermal growth factor, after forming homogeneous mixture, add 8g to and improve looks in emulsion matrix;
2) constantly stir with dasher under rotating speed is 400rpm condition and be cooled to room temperature, the epidermis obtained containing the mesoporous silicon carrying epidermal growth factor repairs breast.
Carry out epidermal growth factor release test to the epidermis reparation breast containing the mesoporous silicon carrying epidermal growth factor that this embodiment obtains, before 10h, drug release rate is very fast, and burst size accounts for 51.3 % of total content.After 24h, release amount accounts for 54.8% of total dose.Without obvious burst effect, epidermal growth factor shows good sustained release performance.
case study on implementation 3:
(1) preparation beauty treatment emulsion matrix:
1) by 16g Rosehips oil, 16g almond oil, 8g liquid paraffin, 6g spermaceti, 4g spermol, the mixing of 4g glyceryl monostearate, be heated to 85 DEG C, under rotating speed is 300rpm condition, carries out magnetic agitation dissolving, obtain A phase;
2) 8g glycerol, 8g triethanolamine are carried out magnetic agitation mixing under rotating speed is 300rpm condition, be heated to 85 DEG C, obtain B phase;
3) be added to by B in A phase, under rotating speed is 300rpm condition, carries out magnetic agitation, be warming up to 95 DEG C and keep 10min to be cooled to 65 DEG C again, then add 29g deionized water, mixing, with high-shear homogenizer homogenizing 15min under 8000rpm speed;
4) remain on 65 DEG C, under 500rpm condition, stir 40min with dasher, after fully emulsified, continue to stir until bubble is all driven away, then add 0.5g antiseptic, 0.5g spice, be cooled to room temperature and obtain emulsion matrix of improving looks;
(2) preparation of the mesoporous silicon of epidermal growth factor is carried:
Take 0.5mg epidermal growth factor to be dissolved in the phosphate buffer of the 0.02M of 100mL and to be placed in seal glass tank, add the hexagonal mesoporous silicon of 15mg, put into 38 DEG C of shaking tables, centrifugal through deionized water wash 3 times, lyophilization 30h in freezer dryer, obtains product and is put in-4 DEG C of refrigerators for subsequent use;
(3) mesoporous silicon and the beauty treatment emulsion matrix that carry epidermal growth factor are composite:
1) at room temperature 0.5g is carried mesoporous silicon and the 2.5g dimethicone agate mortar mix homogeneously of epidermal growth factor, after forming homogeneous mixture, add 10g to and improve looks in emulsion matrix;
2) constantly stir with dasher under rotating speed is 500rpm condition and be cooled to room temperature, the epidermis obtained containing the mesoporous silicon carrying epidermal growth factor repairs breast.
Carry out epidermal growth factor release test to the epidermis reparation breast containing the mesoporous silicon carrying epidermal growth factor that this embodiment obtains, before 10h, drug release rate is very fast, and burst size accounts for 54.7 % of total content.After 24h, release amount accounts for 57.3% of total dose.Without obvious burst effect, epidermal growth factor shows good sustained release performance.
case study on implementation 4:
The epidermis reparation breast containing the mesoporous silicon carrying epidermal growth factor case being implemented 1,2,3 preparations carries out stability experiment, and experimental technique is as follows:
1, dewatering ability: put into by reparation breast with the centrifugal 30min of centrifuge 4000r/min, repairs breast and stablizes not stratified, keep clear, illustrates that the newborn dewatering ability of reparation is good.
2, low-temperature stability: will repair breast and place 30 days in 4 DEG C of refrigerators, and observe after recovering room temperature, repairs breast and stablizes not stratified, illustrates that the newborn low-temperature stability of reparation is good.
3, temperature cycles stability: reparation breast is placed 24h in 4 DEG C of refrigerators, then place 24h in 40 DEG C of baking ovens, circulate 30 days, repairs breast and stablizes not stratified, illustrates that the newborn dewatering ability of reparation is good.
4, accelerated stability: place 3 months for 40 DEG C, respectively at 1,2, March observes, repair breast and stablize not stratified, illustrates that the newborn accelerated stability of reparation is good.
5, long-time stability: be sealed in finger bottle, room temperature places 6 months, respectively at 1,2,3,4,5, June observes, repair breast and stablize not stratified, illustrates that the newborn long-time stability of reparation are good.
Experimental result sees the following form:
| Stability | Phenomenon | Conclusion |
| Dewatering ability | Keep clear, stablize not stratified | Dewatering ability is good |
| Low-temperature stability | Keep clear, stablize not stratified | Low-temperature stability is good |
| Temperature cycles stability | Keep clear, stablize not stratified | Temperature cycles good stability |
| Accelerated stability | Keep clear, stablize not stratified | Accelerated stability is good |
| Long-time stability | Keep clear, stablize not stratified | Long-time stability are good |
case study on implementation 5:
Newborn measure of merit is repaired to the epidermis of a kind of mesoporous silicon containing carrying epidermal growth factor executed prepared by case 1:
For understanding the result of use of product of the present invention, respectively investigation on probation is widely launched to the effect of wrinkle removing, skin care, anti-acne with the form of questionnaire survey.
Select trier 50 people to investigate the effect of its wrinkle removing, wherein men and women half and half, 40 ~ 50 years old age, regain 50 parts of questionnaires altogether.Be applied to face and exposed part after clean of every day in morning, once a day, adhere to 60 days.
Select trier 50 people to investigate the effect of its skin care, wherein men and women half and half, 30 ~ 48 years old age, regain 50 parts of questionnaires altogether.Be applied to face and exposed part after clean of every day in morning, once a day, adhere to 60 days.
Select trier 100 people to investigate the effect of its anti-acne, wherein men and women half and half, 16 ~ 25 years old age, regain 98 parts of questionnaires altogether.Be applied to face and exposed part after clean of every day in morning, once a day, adhere to 60 days.
According to range estimation and the sense learning through practice of trier, indices and result of use self evaluation result as follows:
1. wrinkle removing effect: the trier of 68% has obvious wrinkle to alleviate light effect, the trier of 20% has slight wrinkle to subtract light use sense, and the trier of 12% is without obvious sensation.
2. skin care effect: the trier of 72% has obviously sensation skin to become careful soft, and the trier of 22% feels that skin has and becomes careful soft a little, and the trier of 6% is without obvious sensation.
3. anti-acne effect: the trier of 53% has obvious anti-acne to disappear the effect of pox, the trier of 35% small pox symptom of feeling has and alleviates or the effect of slight anti-acne, and the trier of 12% is without obvious sensation.
4. safety: the trier of 100% is without anaphylaxis.
Same, corresponding investigation on probation has been done simultaneously for case study on implementation 2,3, the efficacy assessments result of survey result and case study on implementation 1 is substantially without significant difference, can find out that the epidermis containing the mesoporous silicon carrying epidermal growth factor repairs breast to no skin irritation, and use comfortable, and the effect having good anti-acne skin care crease-resistant, reach the effect of looks improving and the skin nourishing.
Claims (3)
1. the epidermis containing the mesoporous silicon carrying epidermal growth factor repairs a newborn preparation method, it is characterized in that, comprises the following steps:
(1) preparation beauty treatment emulsion matrix:
1) by Rosehips oil, almond oil, liquid paraffin, spermaceti, spermol, glyceryl monostearate mixing, be heated to 75 ~ 85 DEG C, under rotating speed is 200 ~ 300rpm condition, carries out magnetic agitation dissolving, obtain A phase;
2) glycerol, triethanolamine are carried out magnetic agitation mixing under rotating speed is 150 ~ 300rpm condition, be heated to 75 ~ 85 DEG C, obtain B phase;
3) B is added in A phase, under rotating speed is 150 ~ 300rpm condition, carries out magnetic agitation, is warming up to 90 ~ 95 DEG C and keeps 8 ~ 10min to be cooled to 60 ~ 65 DEG C again, then add deionized water, mixing, with high-shear homogenizer homogenizing 10 ~ 15min under 5000 ~ 8000rpm speed;
4) remain on 60 ~ 65 DEG C, under 300 ~ 500rpm condition, stir 30 ~ 40min with dasher, after fully emulsified, continue to stir until bubble is all driven away, then add antiseptic, spice, be cooled to room temperature and obtain emulsion matrix of improving looks;
(2) preparation of the mesoporous silicon of epidermal growth factor is carried:
Take epidermal growth factor to be dissolved in the phosphate buffer of 0.02M and to be placed in seal glass tank, add hexagonal mesoporous silicon, put into 36 ~ 38 DEG C of shaking tables, centrifugal through deionized water wash 3 times, lyophilization 24 ~ 30h in freezer dryer, obtain the mesoporous silicon carrying epidermal growth factor, be put in-4 DEG C of refrigerators for subsequent use;
(3) mesoporous silicon and the beauty treatment emulsion matrix that carry epidermal growth factor are composite:
1) at room temperature will carry mesoporous silicon and the dimethicone agate mortar mix homogeneously of epidermal growth factor, after forming homogeneous mixture, add in beauty treatment emulsion matrix;
2) constantly stir with dasher under rotating speed is 300 ~ 500rpm condition and be cooled to room temperature, the epidermis obtained containing the mesoporous silicon carrying epidermal growth factor repairs breast;
Described beauty treatment emulsion matrix comprises the emulsion matrix of following mass percentage material composition: Rosehips oil 8% ~ 16%, almond oil 10% ~ 16%, liquid paraffin 6% ~ 8%, spermaceti 4% ~ 6%, spermol 2% ~ 4%, glyceryl monostearate 2% ~ 4%, glycerol 6% ~ 8%, triethanolamine 4% ~ 8%, antiseptic 0.3% ~ 0.5%, spice 0.5% ~ 0.7%, deionized water 29% ~ 57%.
2. the epidermis of a kind of mesoporous silicon containing carrying epidermal growth factor according to claim 1 repairs the preparation method of breast, and it is characterized in that, described in step (2), the concentration of epidermal growth factor in phosphate buffer is 3 ~ 5mg/L; The mass ratio of described epidermal growth factor and mesoporous silicon is 1:30 ~ 50.
3. the epidermis of a kind of mesoporous silicon containing carrying epidermal growth factor prepared by preparation method described in claim 1 or 2 repairs breast, it is characterized in that, described epidermis reparation breast comprises beauty treatment emulsion matrix, the mesoporous silicon carrying epidermal growth factor and dimethicone, and emulsion matrix of wherein improving looks, year mesoporous silicon of epidermal growth factor, the mass ratio of dimethicone are 60 ~ 100:1 ~ 5:5 ~ 25.
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| CN102380102A (en) * | 2011-11-02 | 2012-03-21 | 东华大学 | Method for preparing environment-responsive mesoporous silicon nanoparticles |
| CN102406556A (en) * | 2011-11-16 | 2012-04-11 | 无限极（中国）有限公司 | Cosmetic composition favorable for keeping biological activity of epidermal growth factor (EGF) |
| CN103271837A (en) * | 2013-06-07 | 2013-09-04 | 王迎峰 | Formula and preparation method for bioactive cosmetic |
| CN103343137A (en) * | 2013-07-10 | 2013-10-09 | 吉林农业大学 | Vegetable oil body skin-care emulsion containing epidermal growth factor active polypeptide |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102380102A (en) * | 2011-11-02 | 2012-03-21 | 东华大学 | Method for preparing environment-responsive mesoporous silicon nanoparticles |
| CN102406556A (en) * | 2011-11-16 | 2012-04-11 | 无限极（中国）有限公司 | Cosmetic composition favorable for keeping biological activity of epidermal growth factor (EGF) |
| CN103271837A (en) * | 2013-06-07 | 2013-09-04 | 王迎峰 | Formula and preparation method for bioactive cosmetic |
| CN103343137A (en) * | 2013-07-10 | 2013-10-09 | 吉林农业大学 | Vegetable oil body skin-care emulsion containing epidermal growth factor active polypeptide |
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Effective date of registration: 20160204 Address after: 510663 Guangdong city of Guangzhou province Luogang District Science City Nanxiang Road No. 62 Patentee after: GUANGZHOU RUI SEN BIOTECHNOLOGY CO., LTD. Address before: 510640 Tianhe District, Guangdong, No. five road, No. 381, Patentee before: South China University of Technology |