CN102272145B - 亲和色谱基质 - Google Patents

亲和色谱基质 Download PDF

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Publication number
CN102272145B
CN102272145B CN201080004708.4A CN201080004708A CN102272145B CN 102272145 B CN102272145 B CN 102272145B CN 201080004708 A CN201080004708 A CN 201080004708A CN 102272145 B CN102272145 B CN 102272145B
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CN
China
Prior art keywords
protein
immunoglobulin
ligand
mab
ligands
Prior art date
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CN201080004708.4A
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English (en)
Chinese (zh)
Other versions
CN102272145A (zh
Inventor
H·J·约翰逊
A·伦格勒夫
R·帕尔姆格伦
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Cytiva Bioprocess R&D AB
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GE Healthcare Bio Sciences AB
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Publication of CN102272145A publication Critical patent/CN102272145A/zh
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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/06—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum
    • C07K16/065—Purification, fragmentation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography
    • B01D15/3804—Affinity chromatography
    • B01D15/3809—Affinity chromatography of the antigen-antibody type, e.g. protein A, G or L chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/281—Sorbents specially adapted for preparative, analytical or investigative chromatography
    • B01J20/286—Phases chemically bonded to a substrate, e.g. to silica or to polymers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3231—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the coating or impregnating layer
    • B01J20/3242—Layers with a functional group, e.g. an affinity material, a ligand, a reactant or a complexing group
    • B01J20/3244—Non-macromolecular compounds
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3231—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the coating or impregnating layer
    • B01J20/3242—Layers with a functional group, e.g. an affinity material, a ligand, a reactant or a complexing group
    • B01J20/3244—Non-macromolecular compounds
    • B01J20/3246—Non-macromolecular compounds having a well defined chemical structure
    • B01J20/3248—Non-macromolecular compounds having a well defined chemical structure the functional group or the linking, spacer or anchoring group as a whole comprising at least one type of heteroatom selected from a nitrogen, oxygen or sulfur, these atoms not being part of the carrier as such
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3231—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the coating or impregnating layer
    • B01J20/3242—Layers with a functional group, e.g. an affinity material, a ligand, a reactant or a complexing group
    • B01J20/3268—Macromolecular compounds
    • B01J20/3272—Polymers obtained by reactions otherwise than involving only carbon to carbon unsaturated bonds
    • B01J20/3274—Proteins, nucleic acids, polysaccharides, antibodies or antigens

Landscapes

  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Organic Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Peptides Or Proteins (AREA)
CN201080004708.4A 2009-01-12 2010-01-11 亲和色谱基质 Active CN102272145B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
SE0900029-0 2009-01-12
SE0900029 2009-01-12
PCT/SE2010/050016 WO2010080065A1 (en) 2009-01-12 2010-01-11 Affinity chromatography matrix

Publications (2)

Publication Number Publication Date
CN102272145A CN102272145A (zh) 2011-12-07
CN102272145B true CN102272145B (zh) 2014-07-30

Family

ID=42316657

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201080004708.4A Active CN102272145B (zh) 2009-01-12 2010-01-11 亲和色谱基质

Country Status (5)

Country Link
US (1) US20120149875A1 (enExample)
EP (1) EP2389386A4 (enExample)
JP (1) JP2012515160A (enExample)
CN (1) CN102272145B (enExample)
WO (1) WO2010080065A1 (enExample)

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SE0200943D0 (sv) * 2002-03-25 2002-03-25 Amersham Biosciences Ab Mutant protein
CN101198698B (zh) 2005-03-31 2014-03-19 中外制药株式会社 通过调节多肽缔合制备多肽的方法
EP3056568B1 (en) 2006-03-31 2021-09-15 Chugai Seiyaku Kabushiki Kaisha Methods for controlling blood pharmacokinetics of antibodies
CN101874042B9 (zh) 2007-09-26 2019-01-01 中外制药株式会社 利用cdr的氨基酸取代来改变抗体等电点的方法
RU2526512C2 (ru) 2007-09-26 2014-08-20 Чугаи Сейяку Кабусики Кайся Модифицированная константная область антитела
US8592555B2 (en) 2008-08-11 2013-11-26 Emd Millipore Corporation Immunoglobulin-binding proteins with improved specificity
EP3757090B1 (en) * 2008-11-10 2024-06-12 Arbutus Biopharma Corporation Novel lipids and compositions for the delivery of therapeutics
SG195555A1 (en) 2008-12-24 2013-12-30 Emd Millipore Corp Caustic stable chromatography ligands
SG10201912450XA (en) * 2009-06-10 2020-03-30 Arbutus Biopharma Corp Improved lipid formulation
JP5917417B2 (ja) * 2010-03-05 2016-05-11 ベーリンガー インゲルハイム インターナショナル ゲゼルシャフト ミット ベシュレンクテル ハフツング 抗体の選択的強化
US8859726B2 (en) 2010-11-29 2014-10-14 Ge Healthcare Bio-Sciences Ab Affinity chromatography matrix
EP4279513A3 (en) 2010-11-30 2024-02-28 Chugai Seiyaku Kabushiki Kaisha Cytotoxicity-inducing therapeutic agent
JP5974342B2 (ja) 2010-12-20 2016-08-23 ジーイー・ヘルスケア・バイオプロセス・アールアンドディ・アクチボラグ アフィニティークロマトグラフィーマトリックス
US9187555B2 (en) * 2010-12-20 2015-11-17 Ge Healthcare Bio-Sciences Ab Affinity chromatography matrix
SG186552A1 (en) 2011-06-08 2013-01-30 Emd Millipore Corp Chromatography matrices including novel staphylococcus aureus protein a based ligands
US20140221613A1 (en) * 2011-08-04 2014-08-07 Daicel Corporation Novel modified protein comprising tandem-type multimer of mutant extracellular domain of protein g
WO2013147691A1 (en) 2012-03-28 2013-10-03 Ge Healthcare Bio-Sciences Ab Affinity chromatography matrix
US8921113B2 (en) 2012-12-21 2014-12-30 Dionex Corporation Buffer kit and method of generating a linear pH gradient
JP6544808B2 (ja) * 2013-07-10 2019-07-17 ジーイー・ヘルスケア・バイオプロセス・アールアンドディ・アクチボラグ 突然変異免疫グロブリン結合ポリペプチド
JP6464089B2 (ja) 2013-09-06 2019-02-06 株式会社カネカ アフィニティー分離マトリックス用分離能強化リガンド
US11566082B2 (en) 2014-11-17 2023-01-31 Cytiva Bioprocess R&D Ab Mutated immunoglobulin-binding polypeptides
WO2016159213A1 (ja) 2015-04-01 2016-10-06 中外製薬株式会社 ポリペプチド異種多量体の製造方法
BR112018009312A8 (pt) * 2015-12-28 2019-02-26 Chugai Pharmaceutical Co Ltd método para promover eficiência de purificação de polipeptídeo contendo região de fc
SG11201807765PA (en) 2016-04-28 2018-10-30 Chugai Pharmaceutical Co Ltd Antibody-containing preparation
US10730908B2 (en) 2016-05-11 2020-08-04 Ge Healthcare Bioprocess R&D Ab Separation method
JP7031934B2 (ja) 2016-05-11 2022-03-08 サイティバ・バイオプロセス・アールアンドディ・アクチボラグ 分離マトリックス
US10889615B2 (en) 2016-05-11 2021-01-12 Cytiva Bioprocess R&D Ab Mutated immunoglobulin-binding polypeptides
US10654887B2 (en) 2016-05-11 2020-05-19 Ge Healthcare Bio-Process R&D Ab Separation matrix
CN109311948B (zh) 2016-05-11 2022-09-16 思拓凡生物工艺研发有限公司 清洁和/或消毒分离基质的方法
EP3455240B1 (en) * 2016-05-11 2025-01-01 Cytiva BioProcess R&D AB Method of storing a separation matrix
US10703774B2 (en) 2016-09-30 2020-07-07 Ge Healthcare Bioprocess R&D Ab Separation method
EP3455243B1 (en) 2016-05-11 2021-03-24 Cytiva BioProcess R&D AB Separation matrix
US12448411B2 (en) 2016-09-30 2025-10-21 Cytiva Bioprocess R&D Ab Separation method
CN106543274B (zh) * 2016-10-18 2020-10-27 天津大学 一种对抗体具有特异性结合作用的a蛋白结构域z的衍生物及其应用
GB201703116D0 (en) * 2017-02-27 2017-04-12 Ge Healthcare Bioprocess R&D Ab A seperation matrix and a method of seperating antibodies
CR20240273A (es) 2017-11-01 2024-08-27 Chugai Pharmaceutical Co Ltd Variantes e isoformas de anticuerpos con actividad biológica reducida (divisional 2020-229)
JP7335881B2 (ja) * 2018-08-24 2023-08-30 Jsr株式会社 イムノグロブリン結合タンパク質、及びそれを用いたアフィニティー担体
CN109182364A (zh) * 2018-08-30 2019-01-11 武汉百意欣生物技术有限公司 一种特异性识别蛋白a的多克隆抗体及其制备方法和应用
US11022585B2 (en) 2019-06-09 2021-06-01 Dionex Corporation Methods and systems for optimizing buffer conditions with liquid chromatography
EP4541444A1 (en) * 2023-10-20 2025-04-23 Cytiva BioProcess R&D AB Fusion protein for affinity capture

Citations (2)

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Publication number Priority date Publication date Assignee Title
US20060194950A1 (en) * 2002-03-25 2006-08-31 Sophia Hober Mutant protein
US20060194955A1 (en) * 2002-03-25 2006-08-31 Sophia Hober Protein ligands

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Publication number Priority date Publication date Assignee Title
ATE333469T1 (de) * 2001-06-05 2006-08-15 Genetics Inst Llc Verfahren zur reinigung hochanionischer proteine
US8592555B2 (en) * 2008-08-11 2013-11-26 Emd Millipore Corporation Immunoglobulin-binding proteins with improved specificity
SG195555A1 (en) * 2008-12-24 2013-12-30 Emd Millipore Corp Caustic stable chromatography ligands

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060194950A1 (en) * 2002-03-25 2006-08-31 Sophia Hober Mutant protein
US20060194955A1 (en) * 2002-03-25 2006-08-31 Sophia Hober Protein ligands

Also Published As

Publication number Publication date
US20120149875A1 (en) 2012-06-14
WO2010080065A1 (en) 2010-07-15
JP2012515160A (ja) 2012-07-05
EP2389386A1 (en) 2011-11-30
CN102272145A (zh) 2011-12-07
EP2389386A4 (en) 2013-11-06

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Patentee after: Situofan Biotechnology R & D Co., Ltd

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