CN102257163A - 在样品中检测目标核酸的方法 - Google Patents

在样品中检测目标核酸的方法 Download PDF

Info

Publication number
CN102257163A
CN102257163A CN2009801517027A CN200980151702A CN102257163A CN 102257163 A CN102257163 A CN 102257163A CN 2009801517027 A CN2009801517027 A CN 2009801517027A CN 200980151702 A CN200980151702 A CN 200980151702A CN 102257163 A CN102257163 A CN 102257163A
Authority
CN
China
Prior art keywords
quencher
nucleic acid
fluorescence
target nucleic
reporter molecule
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN2009801517027A
Other languages
English (en)
Chinese (zh)
Inventor
N.牛顿
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
F Hoffmann La Roche AG
Original Assignee
F Hoffmann La Roche AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by F Hoffmann La Roche AG filed Critical F Hoffmann La Roche AG
Publication of CN102257163A publication Critical patent/CN102257163A/zh
Pending legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN2009801517027A 2008-12-22 2009-12-17 在样品中检测目标核酸的方法 Pending CN102257163A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US12/341130 2008-12-22
US12/341,130 US20100159452A1 (en) 2008-12-22 2008-12-22 Method For Detecting a Target Nucleic Acid in a Sample
PCT/EP2009/009053 WO2010072366A1 (en) 2008-12-22 2009-12-17 Method for detecting a target nucleic acid in a sample

Publications (1)

Publication Number Publication Date
CN102257163A true CN102257163A (zh) 2011-11-23

Family

ID=41800824

Family Applications (1)

Application Number Title Priority Date Filing Date
CN2009801517027A Pending CN102257163A (zh) 2008-12-22 2009-12-17 在样品中检测目标核酸的方法

Country Status (6)

Country Link
US (1) US20100159452A1 (enExample)
EP (1) EP2379741B1 (enExample)
JP (1) JP2012513191A (enExample)
CN (1) CN102257163A (enExample)
CA (1) CA2746716A1 (enExample)
WO (1) WO2010072366A1 (enExample)

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102471805A (zh) * 2009-08-14 2012-05-23 霍夫曼-拉罗奇有限公司 检测核酸差异的探针形式
CN105300942A (zh) * 2015-10-29 2016-02-03 锦州奥鸿药业有限责任公司 一种检测小牛血清去蛋白注射液中单磷酸腺苷含量的方法
CN108410964A (zh) * 2017-02-10 2018-08-17 豪夫迈·罗氏有限公司 一种用于减小数字聚合酶链式反应中由光学假象引起的量化误差的方法
CN108531549A (zh) * 2017-03-03 2018-09-14 长庚大学 核酸分子检测方法及检测试剂盒
CN108642165A (zh) * 2018-05-22 2018-10-12 美林美邦（厦门）生物科技有限公司 一种用于实时荧光pcr的探针及其使用方法
CN110621783A (zh) * 2017-05-25 2019-12-27 豪夫迈·罗氏有限公司 多重核酸扩增测定
CN111683897A (zh) * 2017-11-03 2020-09-18 华盛顿大学 使用编码颗粒的数字核酸扩增

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10273525B2 (en) 2010-11-10 2019-04-30 Brandeis University Compositions, methods, and kits for detecting and identifying mycobacteria
CN108342453A (zh) 2011-05-09 2018-07-31 富鲁达公司 基于探针的核酸检测
US9644231B2 (en) 2011-05-09 2017-05-09 Fluidigm Corporation Nucleic acid detection using probes
WO2014077822A1 (en) * 2012-11-15 2014-05-22 Fluidigm Corporation Nucleic acid detection using probes

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5861124A (en) * 1993-07-15 1999-01-19 Hamamatsu Photonics Kk Method and apparatus for detecting denaturation of nucleic acid
US6174670B1 (en) * 1996-06-04 2001-01-16 University Of Utah Research Foundation Monitoring amplification of DNA during PCR
WO2004074447A2 (en) * 2003-02-18 2004-09-02 Applera Corporation Compositions and methods for multiplex analysis of polynucleotides
WO2005090610A2 (en) * 2004-03-18 2005-09-29 Advandx, Inc. Methods, kits and compositions pertaining to nucleic acid probes using fluorescence quenching
CN1727895A (zh) * 2004-07-30 2006-02-01 王进科 核酸酶消化fret标记核酸探针检测转录因子蛋白
EP1780291A1 (en) * 2006-03-01 2007-05-02 Institut Biokhimii I Genetiki Ufimskogo Nauchnogo Tsentra Ran Method of detecting specific fragments of DNA or RNA with the aid of a polymerase chain reaction
US20070248994A1 (en) * 2004-03-17 2007-10-25 University Of Hawaii Sensor constructs and detection methods

Family Cites Families (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5466591A (en) * 1986-08-22 1995-11-14 Hoffmann-La Roche Inc. 5' to 3' exonuclease mutations of thermostable DNA polymerases
US5366860A (en) * 1989-09-29 1994-11-22 Applied Biosystems, Inc. Spectrally resolvable rhodamine dyes for nucleic acid sequence determination
US5188934A (en) * 1989-11-14 1993-02-23 Applied Biosystems, Inc. 4,7-dichlorofluorescein dyes as molecular probes
EP0550687B1 (en) * 1990-09-28 1999-06-09 F. Hoffmann-La Roche Ag 5' to 3' exonuclease mutations of thermostable dna polymerases
US5994056A (en) * 1991-05-02 1999-11-30 Roche Molecular Systems, Inc. Homogeneous methods for nucleic acid amplification and detection
US5538848A (en) * 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
US6020481A (en) * 1996-04-01 2000-02-01 The Perkin-Elmer Corporation Asymmetric benzoxanthene dyes
US5863727A (en) * 1996-05-03 1999-01-26 The Perkin-Elmer Corporation Energy transfer dyes with enhanced fluorescence
US5945526A (en) * 1996-05-03 1999-08-31 Perkin-Elmer Corporation Energy transfer dyes with enhanced fluorescence
US5800996A (en) * 1996-05-03 1998-09-01 The Perkin Elmer Corporation Energy transfer dyes with enchanced fluorescence
US5847162A (en) * 1996-06-27 1998-12-08 The Perkin Elmer Corporation 4, 7-Dichlororhodamine dyes
JP2001514859A (ja) * 1997-09-04 2001-09-18 バイエル コーポレイション クエンチ可能な蛍光標識を有するオリゴヌクレオチドプローブおよびその使用方法
US6008379A (en) * 1997-10-01 1999-12-28 The Perkin-Elmer Corporation Aromatic-substituted xanthene dyes
US5936087A (en) * 1997-11-25 1999-08-10 The Perkin-Elmer Corporation Dibenzorhodamine dyes
EP1246935B1 (en) * 1999-09-28 2013-08-14 Geneohm Sciences Canada Inc. Highly conserved genes and their use to generate probes and primers for detection of microorganisms
US6191278B1 (en) * 1999-11-03 2001-02-20 Pe Corporation Water-soluble rhodamine dyes and conjugates thereof
US6960436B2 (en) * 2002-02-06 2005-11-01 Epigenomics Ag Quantitative methylation detection in DNA samples
US20040022764A1 (en) * 2002-07-31 2004-02-05 Hanan Polansky Inhibition of microcompetition with a foreign polynucleotide as treatment of chronic disease
JP4235902B2 (ja) * 2002-09-02 2009-03-11 東洋紡績株式会社 塩基多型の同定方法
US20090162863A1 (en) * 2007-12-13 2009-06-25 Hitachi High-Technologies Corporation Nucleic acid detection probe

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5861124A (en) * 1993-07-15 1999-01-19 Hamamatsu Photonics Kk Method and apparatus for detecting denaturation of nucleic acid
US6174670B1 (en) * 1996-06-04 2001-01-16 University Of Utah Research Foundation Monitoring amplification of DNA during PCR
WO2004074447A2 (en) * 2003-02-18 2004-09-02 Applera Corporation Compositions and methods for multiplex analysis of polynucleotides
US20070248994A1 (en) * 2004-03-17 2007-10-25 University Of Hawaii Sensor constructs and detection methods
WO2005090610A2 (en) * 2004-03-18 2005-09-29 Advandx, Inc. Methods, kits and compositions pertaining to nucleic acid probes using fluorescence quenching
CN1727895A (zh) * 2004-07-30 2006-02-01 王进科 核酸酶消化fret标记核酸探针检测转录因子蛋白
EP1780291A1 (en) * 2006-03-01 2007-05-02 Institut Biokhimii I Genetiki Ufimskogo Nauchnogo Tsentra Ran Method of detecting specific fragments of DNA or RNA with the aid of a polymerase chain reaction

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
DE SILVA ET AL.: "Rapid genotyping and quantification on the lightCyclerTM with hybridization probes", 《BIOCHEMICA》 *

Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102471805A (zh) * 2009-08-14 2012-05-23 霍夫曼-拉罗奇有限公司 检测核酸差异的探针形式
CN105300942A (zh) * 2015-10-29 2016-02-03 锦州奥鸿药业有限责任公司 一种检测小牛血清去蛋白注射液中单磷酸腺苷含量的方法
CN105300942B (zh) * 2015-10-29 2017-11-21 锦州奥鸿药业有限责任公司 一种检测小牛血清去蛋白注射液中单磷酸腺苷含量的方法
CN108410964A (zh) * 2017-02-10 2018-08-17 豪夫迈·罗氏有限公司 一种用于减小数字聚合酶链式反应中由光学假象引起的量化误差的方法
CN108410964B (zh) * 2017-02-10 2023-08-25 豪夫迈·罗氏有限公司 一种用于减小数字聚合酶链式反应中由光学假象引起的量化误差的方法
CN108531549A (zh) * 2017-03-03 2018-09-14 长庚大学 核酸分子检测方法及检测试剂盒
CN110621783A (zh) * 2017-05-25 2019-12-27 豪夫迈·罗氏有限公司 多重核酸扩增测定
CN110621783B (zh) * 2017-05-25 2024-05-10 豪夫迈·罗氏有限公司 多重核酸扩增测定
CN111683897A (zh) * 2017-11-03 2020-09-18 华盛顿大学 使用编码颗粒的数字核酸扩增
CN108642165A (zh) * 2018-05-22 2018-10-12 美林美邦（厦门）生物科技有限公司 一种用于实时荧光pcr的探针及其使用方法

Also Published As

Publication number Publication date
WO2010072366A1 (en) 2010-07-01
EP2379741A1 (en) 2011-10-26
US20100159452A1 (en) 2010-06-24
CA2746716A1 (en) 2010-07-01
JP2012513191A (ja) 2012-06-14
EP2379741B1 (en) 2016-03-23

Similar Documents

Publication Publication Date Title
CN102257163A (zh) 在样品中检测目标核酸的方法
JP4540844B2 (ja) 核酸の蛍光定量的検出システム
JP5112592B2 (ja) ハイブリダイゼーション・ビーコン、並びに迅速に配列を検出および判別する方法
CN101680029B (zh) 核酸检测
JP4540754B2 (ja) Ｐｃｒ中のハイブリダイゼーションのモニタリング
JP5543065B2 (ja) 核酸増幅のためのプライマー、プローブおよび方法
CN101831496B (zh) 多重定量核酸扩增及解链测定法
JP5354216B2 (ja) 核酸測定用の核酸プローブ、核酸プローブセットおよびそれらを用いる標的核酸の測定方法
JP5235905B2 (ja) 核酸配列の検出方法
US20100227326A1 (en) Detection System
HU226518B1 (en) Method for detection of target nucleic acids using pcr
JP2004033216A (ja) アンプリコン融解曲線分析による遺伝子型分析
CA2340458A1 (en) Fluorescence polarization in nucleic acid analysis
CN102171366A (zh) 多重扩增与检测
AU2002311414A1 (en) Nucleic acid detection method
CN105431550A (zh) 多重等位基因检测
US20040214176A1 (en) Multiplexed DNA assays using structure-specific endonucleases
JP2004313120A (ja) β３アドレナリン受容体変異遺伝子の検出法ならびにそのための核酸プローブおよびキット
HK1161746B (en) Multiplex amplification and detection

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C12 Rejection of a patent application after its publication
RJ01 Rejection of invention patent application after publication

Application publication date: 20111123