JP2012513191A - 試料中の標的核酸を検出するための方法 - Google Patents

試料中の標的核酸を検出するための方法 Download PDF

Info

Publication number
JP2012513191A
JP2012513191A JP2011541207A JP2011541207A JP2012513191A JP 2012513191 A JP2012513191 A JP 2012513191A JP 2011541207 A JP2011541207 A JP 2011541207A JP 2011541207 A JP2011541207 A JP 2011541207A JP 2012513191 A JP2012513191 A JP 2012513191A
Authority
JP
Japan
Prior art keywords
nucleic acid
quencher
reporter
target nucleic
fluorescence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP2011541207A
Other languages
English (en)
Japanese (ja)
Other versions
JP2012513191A5 (enExample
Inventor
ニュートン ニコラス
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
F Hoffmann La Roche AG
Original Assignee
F Hoffmann La Roche AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by F Hoffmann La Roche AG filed Critical F Hoffmann La Roche AG
Publication of JP2012513191A publication Critical patent/JP2012513191A/ja
Publication of JP2012513191A5 publication Critical patent/JP2012513191A5/ja
Pending legal-status Critical Current

Links

Images

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6816Hybridisation assays characterised by the detection means
    • C12Q1/6818Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2011541207A 2008-12-22 2009-12-17 試料中の標的核酸を検出するための方法 Pending JP2012513191A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US12/341,130 US20100159452A1 (en) 2008-12-22 2008-12-22 Method For Detecting a Target Nucleic Acid in a Sample
US12/341,130 2008-12-22
PCT/EP2009/009053 WO2010072366A1 (en) 2008-12-22 2009-12-17 Method for detecting a target nucleic acid in a sample

Publications (2)

Publication Number Publication Date
JP2012513191A true JP2012513191A (ja) 2012-06-14
JP2012513191A5 JP2012513191A5 (enExample) 2012-08-16

Family

ID=41800824

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2011541207A Pending JP2012513191A (ja) 2008-12-22 2009-12-17 試料中の標的核酸を検出するための方法

Country Status (6)

Country Link
US (1) US20100159452A1 (enExample)
EP (1) EP2379741B1 (enExample)
JP (1) JP2012513191A (enExample)
CN (1) CN102257163A (enExample)
CA (1) CA2746716A1 (enExample)
WO (1) WO2010072366A1 (enExample)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8409802B2 (en) * 2009-08-14 2013-04-02 Roche Molecular Systems, Inc. Format of probes to detect nucleic acid differences
EP3135775B1 (en) * 2010-11-10 2018-06-27 Brandeis University Kit for detecting and identifying mycobacteria
US9644231B2 (en) 2011-05-09 2017-05-09 Fluidigm Corporation Nucleic acid detection using probes
WO2012154876A1 (en) 2011-05-09 2012-11-15 Fluidigm Corporation Probe based nucleic acid detection
WO2014077822A1 (en) * 2012-11-15 2014-05-22 Fluidigm Corporation Nucleic acid detection using probes
CN105300942B (zh) * 2015-10-29 2017-11-21 锦州奥鸿药业有限责任公司 一种检测小牛血清去蛋白注射液中单磷酸腺苷含量的方法
EP3360970B1 (en) * 2017-02-10 2019-10-30 Roche Diagnostics GmbH A method for reducing quantification errors caused by an optical artefact in digital polymerase chain reaction
TWI617667B (zh) * 2017-03-03 2018-03-11 長庚大學 核酸分子檢測方法及檢測套組
US20190093155A1 (en) * 2017-05-25 2019-03-28 Roche Molecular Systems, Inc. Multiplex Nucleic Acid Amplification Assay
WO2019089996A1 (en) * 2017-11-03 2019-05-09 University Of Washington Digital nucleic acid amplification using encoded particles
CN108642165A (zh) * 2018-05-22 2018-10-12 美林美邦(厦门)生物科技有限公司 一种用于实时荧光pcr的探针及其使用方法

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH05506364A (ja) * 1990-09-28 1993-09-22 エフ.ホフマンーラ ロシュ アーゲー 熱安定性 dnaポリメラーゼの5→3′のエキソヌクレアーゼ突然変異
JP2000509608A (ja) * 1996-06-04 2000-08-02 ユニバーシティ オブ ユタ リサーチ ファウンデーション Pcr中のハイブリダイゼーションのモニタリング
JP2004121232A (ja) * 2002-09-02 2004-04-22 Toyobo Co Ltd 塩基多型の同定方法
JP2005516628A (ja) * 2002-02-06 2005-06-09 エピゲノミクス アーゲー Dnaサンプルの定量的メチル化検出

Family Cites Families (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5466591A (en) * 1986-08-22 1995-11-14 Hoffmann-La Roche Inc. 5' to 3' exonuclease mutations of thermostable DNA polymerases
US5366860A (en) * 1989-09-29 1994-11-22 Applied Biosystems, Inc. Spectrally resolvable rhodamine dyes for nucleic acid sequence determination
US5188934A (en) * 1989-11-14 1993-02-23 Applied Biosystems, Inc. 4,7-dichlorofluorescein dyes as molecular probes
US5994056A (en) * 1991-05-02 1999-11-30 Roche Molecular Systems, Inc. Homogeneous methods for nucleic acid amplification and detection
US5861124A (en) * 1993-07-15 1999-01-19 Hamamatsu Photonics Kk Method and apparatus for detecting denaturation of nucleic acid
US5538848A (en) * 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
US6020481A (en) * 1996-04-01 2000-02-01 The Perkin-Elmer Corporation Asymmetric benzoxanthene dyes
US5863727A (en) * 1996-05-03 1999-01-26 The Perkin-Elmer Corporation Energy transfer dyes with enhanced fluorescence
US5945526A (en) * 1996-05-03 1999-08-31 Perkin-Elmer Corporation Energy transfer dyes with enhanced fluorescence
US5847162A (en) * 1996-06-27 1998-12-08 The Perkin Elmer Corporation 4, 7-Dichlororhodamine dyes
US5800996A (en) * 1996-05-03 1998-09-01 The Perkin Elmer Corporation Energy transfer dyes with enchanced fluorescence
WO1999011813A2 (en) * 1997-09-04 1999-03-11 Bayer Corporation Oligonucleotide probes bearing quenchable fluorescent labels, and methods of use thereof
US6008379A (en) * 1997-10-01 1999-12-28 The Perkin-Elmer Corporation Aromatic-substituted xanthene dyes
US5936087A (en) * 1997-11-25 1999-08-10 The Perkin-Elmer Corporation Dibenzorhodamine dyes
CA2388461C (en) * 1999-09-28 2013-06-11 Infectio Diagnostic (I.D.I.) Inc. Highly conserved genes and their use to generate probes and primers for the detection of microorganisms
US6191278B1 (en) * 1999-11-03 2001-02-20 Pe Corporation Water-soluble rhodamine dyes and conjugates thereof
US20040022764A1 (en) * 2002-07-31 2004-02-05 Hanan Polansky Inhibition of microcompetition with a foreign polynucleotide as treatment of chronic disease
CA2516299A1 (en) * 2003-02-18 2004-09-02 Applera Corporation Compositions and methods for multiplex analysis of polynucleotides
EP1759186A4 (en) * 2004-03-17 2009-02-11 Univ Hawaii SENSOR CONSTRUCTS AND DETECTION METHODS
US20090143237A1 (en) * 2004-03-18 2009-06-04 Advandx, Inc. Methods, kits and compositions pertaining to fluorescence quenching using pna probes
CN1727895A (zh) * 2004-07-30 2006-02-01 王进科 核酸酶消化fret标记核酸探针检测转录因子蛋白
RU2005132940A (ru) * 2006-03-01 2007-11-27 Институт биохимии и генетики Уфимского научного центра РАН (RU) Способ детекции специфических фрагментов днк или рнк с помощью полимеразной цепной реакции в режиме реального времени
US20090162863A1 (en) * 2007-12-13 2009-06-25 Hitachi High-Technologies Corporation Nucleic acid detection probe

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH05506364A (ja) * 1990-09-28 1993-09-22 エフ.ホフマンーラ ロシュ アーゲー 熱安定性 dnaポリメラーゼの5→3′のエキソヌクレアーゼ突然変異
JP2000509608A (ja) * 1996-06-04 2000-08-02 ユニバーシティ オブ ユタ リサーチ ファウンデーション Pcr中のハイブリダイゼーションのモニタリング
JP2005516628A (ja) * 2002-02-06 2005-06-09 エピゲノミクス アーゲー Dnaサンプルの定量的メチル化検出
JP2004121232A (ja) * 2002-09-02 2004-04-22 Toyobo Co Ltd 塩基多型の同定方法

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
JPN6014003607; J. Forensic Sci. (Nov 2008) vol.53, no.6, p.1316-1324 *
JPN6014003608; Roche LightCycler 480 Genotyping Master (Feb 2008)<URL: https://cssportal.roche.com/LFR_PublicDocs/r *

Also Published As

Publication number Publication date
CA2746716A1 (en) 2010-07-01
CN102257163A (zh) 2011-11-23
US20100159452A1 (en) 2010-06-24
WO2010072366A1 (en) 2010-07-01
EP2379741B1 (en) 2016-03-23
EP2379741A1 (en) 2011-10-26

Similar Documents

Publication Publication Date Title
EP2379741B1 (en) Method for detecting a target nucleic acid in a sample
CN101831496B (zh) 多重定量核酸扩增及解链测定法
JP5112592B2 (ja) ハイブリダイゼーション・ビーコン、並びに迅速に配列を検出および判別する方法
CN102171366B (zh) 多重扩增与检测
EP3394287B1 (en) A method of fluorescent detection of isothermal loop-mediated amplification (lamp) of a target nucleic acid
JP4972541B2 (ja) 標識されたプローブおよび3’→5’エキソヌクレアーゼ活性を用いた定量的増幅方法
JP6021813B2 (ja) 多重増幅および検出
JP5770738B2 (ja) Thdプライマーターゲット検出
EP2438189B1 (en) Multiplex amplification and detection
US20140274779A1 (en) Multiplex allele detection
KR20040024556A (ko) 검출 시스템
US20070231808A1 (en) Methods of nucleic acid analysis by single molecule detection
ES2980651T3 (es) Método de detección de una secuencia de ácido nucleico
US9689026B2 (en) Detecting single nucleotide polymorphism using overlapping hydrolysis probes
US20060194222A1 (en) Triplex probe compositions and methods for polynucleotide detection
Tang et al. A single quantum dot-based biosensor for DNA point mutation assay
TWI617667B (zh) 核酸分子檢測方法及檢測套組
JP2004313120A (ja) β3アドレナリン受容体変異遺伝子の検出法ならびにそのための核酸プローブおよびキット
HK1161746B (en) Multiplex amplification and detection
HK1262086A1 (en) A method of fluorescent detection of isothermal loop-mediated amplification (lamp) of a target nucleic acid
HK1262086B (en) A method of fluorescent detection of isothermal loop-mediated amplification (lamp) of a target nucleic acid
WO2017044651A2 (en) Short oligonucleotide quenchers for reduction of taqman probe baseline fluorescence

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20120625

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20120625

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20140204

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20140410

A602 Written permission of extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A602

Effective date: 20140417

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20140804

A02 Decision of refusal

Free format text: JAPANESE INTERMEDIATE CODE: A02

Effective date: 20150303