JP2012513191A5 - - Google Patents

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Publication number
JP2012513191A5
JP2012513191A5 JP2011541207A JP2011541207A JP2012513191A5 JP 2012513191 A5 JP2012513191 A5 JP 2012513191A5 JP 2011541207 A JP2011541207 A JP 2011541207A JP 2011541207 A JP2011541207 A JP 2011541207A JP 2012513191 A5 JP2012513191 A5 JP 2012513191A5
Authority
JP
Japan
Prior art keywords
nucleic acid
quencher
target nucleic
fluorescent reporter
thermus
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP2011541207A
Other languages
English (en)
Japanese (ja)
Other versions
JP2012513191A (ja
Filing date
Publication date
Priority claimed from US12/341,130 external-priority patent/US20100159452A1/en
Application filed filed Critical
Publication of JP2012513191A publication Critical patent/JP2012513191A/ja
Publication of JP2012513191A5 publication Critical patent/JP2012513191A5/ja
Pending legal-status Critical Current

Links

JP2011541207A 2008-12-22 2009-12-17 試料中の標的核酸を検出するための方法 Pending JP2012513191A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US12/341,130 US20100159452A1 (en) 2008-12-22 2008-12-22 Method For Detecting a Target Nucleic Acid in a Sample
US12/341,130 2008-12-22
PCT/EP2009/009053 WO2010072366A1 (en) 2008-12-22 2009-12-17 Method for detecting a target nucleic acid in a sample

Publications (2)

Publication Number Publication Date
JP2012513191A JP2012513191A (ja) 2012-06-14
JP2012513191A5 true JP2012513191A5 (enExample) 2012-08-16

Family

ID=41800824

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2011541207A Pending JP2012513191A (ja) 2008-12-22 2009-12-17 試料中の標的核酸を検出するための方法

Country Status (6)

Country Link
US (1) US20100159452A1 (enExample)
EP (1) EP2379741B1 (enExample)
JP (1) JP2012513191A (enExample)
CN (1) CN102257163A (enExample)
CA (1) CA2746716A1 (enExample)
WO (1) WO2010072366A1 (enExample)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8409802B2 (en) * 2009-08-14 2013-04-02 Roche Molecular Systems, Inc. Format of probes to detect nucleic acid differences
EP2638178B1 (en) * 2010-11-10 2016-10-05 Brandeis University Methods, and kits for detecting and identifying mycobacteria
WO2014077822A1 (en) * 2012-11-15 2014-05-22 Fluidigm Corporation Nucleic acid detection using probes
US9644231B2 (en) 2011-05-09 2017-05-09 Fluidigm Corporation Nucleic acid detection using probes
SG194722A1 (en) 2011-05-09 2013-12-30 Fluidigm Corp Probe based nucleic acid detection
CN105300942B (zh) * 2015-10-29 2017-11-21 锦州奥鸿药业有限责任公司 一种检测小牛血清去蛋白注射液中单磷酸腺苷含量的方法
EP3360970B1 (en) * 2017-02-10 2019-10-30 Roche Diagnostics GmbH A method for reducing quantification errors caused by an optical artefact in digital polymerase chain reaction
TWI617667B (zh) * 2017-03-03 2018-03-11 長庚大學 核酸分子檢測方法及檢測套組
US20190093155A1 (en) * 2017-05-25 2019-03-28 Roche Molecular Systems, Inc. Multiplex Nucleic Acid Amplification Assay
WO2019089996A1 (en) * 2017-11-03 2019-05-09 University Of Washington Digital nucleic acid amplification using encoded particles
CN108642165A (zh) * 2018-05-22 2018-10-12 美林美邦（厦门）生物科技有限公司 一种用于实时荧光pcr的探针及其使用方法
CN119365596A (zh) * 2022-06-20 2025-01-24 凸版控股株式会社 用于检测荧光的方法

Family Cites Families (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5466591A (en) * 1986-08-22 1995-11-14 Hoffmann-La Roche Inc. 5' to 3' exonuclease mutations of thermostable DNA polymerases
US5366860A (en) * 1989-09-29 1994-11-22 Applied Biosystems, Inc. Spectrally resolvable rhodamine dyes for nucleic acid sequence determination
US5188934A (en) * 1989-11-14 1993-02-23 Applied Biosystems, Inc. 4,7-dichlorofluorescein dyes as molecular probes
EP0894860B1 (en) * 1990-09-28 2012-12-12 F. Hoffmann-La Roche AG Thermostable DNA polymerases and deletion mutants thereof
US5994056A (en) * 1991-05-02 1999-11-30 Roche Molecular Systems, Inc. Homogeneous methods for nucleic acid amplification and detection
US5861124A (en) * 1993-07-15 1999-01-19 Hamamatsu Photonics Kk Method and apparatus for detecting denaturation of nucleic acid
US5538848A (en) * 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
US6020481A (en) * 1996-04-01 2000-02-01 The Perkin-Elmer Corporation Asymmetric benzoxanthene dyes
US5847162A (en) * 1996-06-27 1998-12-08 The Perkin Elmer Corporation 4, 7-Dichlororhodamine dyes
US5800996A (en) * 1996-05-03 1998-09-01 The Perkin Elmer Corporation Energy transfer dyes with enchanced fluorescence
US5863727A (en) * 1996-05-03 1999-01-26 The Perkin-Elmer Corporation Energy transfer dyes with enhanced fluorescence
US5945526A (en) * 1996-05-03 1999-08-31 Perkin-Elmer Corporation Energy transfer dyes with enhanced fluorescence
ATE428801T1 (de) * 1996-06-04 2009-05-15 Univ Utah Res Found Überwachung der hybridisierung während pcr
WO1999011813A2 (en) * 1997-09-04 1999-03-11 Bayer Corporation Oligonucleotide probes bearing quenchable fluorescent labels, and methods of use thereof
US6008379A (en) * 1997-10-01 1999-12-28 The Perkin-Elmer Corporation Aromatic-substituted xanthene dyes
US5936087A (en) * 1997-11-25 1999-08-10 The Perkin-Elmer Corporation Dibenzorhodamine dyes
EP1246935B1 (en) * 1999-09-28 2013-08-14 Geneohm Sciences Canada Inc. Highly conserved genes and their use to generate probes and primers for detection of microorganisms
US6191278B1 (en) * 1999-11-03 2001-02-20 Pe Corporation Water-soluble rhodamine dyes and conjugates thereof
US6960436B2 (en) * 2002-02-06 2005-11-01 Epigenomics Ag Quantitative methylation detection in DNA samples
US20040022764A1 (en) * 2002-07-31 2004-02-05 Hanan Polansky Inhibition of microcompetition with a foreign polynucleotide as treatment of chronic disease
JP4235902B2 (ja) * 2002-09-02 2009-03-11 東洋紡績株式会社 塩基多型の同定方法
WO2004074447A2 (en) * 2003-02-18 2004-09-02 Applera Corporation Compositions and methods for multiplex analysis of polynucleotides
JP2007529747A (ja) * 2004-03-17 2007-10-25 ユニバーシティ オブ ハワイ センサ構造物及び検出方法
US20090143237A1 (en) * 2004-03-18 2009-06-04 Advandx, Inc. Methods, kits and compositions pertaining to fluorescence quenching using pna probes
CN1727895A (zh) * 2004-07-30 2006-02-01 王进科 核酸酶消化fret标记核酸探针检测转录因子蛋白
RU2005132940A (ru) * 2006-03-01 2007-11-27 Институт биохимии и генетики Уфимского научного центра РАН (RU) Способ детекции специфических фрагментов днк или рнк с помощью полимеразной цепной реакции в режиме реального времени
US20090162863A1 (en) * 2007-12-13 2009-06-25 Hitachi High-Technologies Corporation Nucleic acid detection probe

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