JP2013540449A5 - - Google Patents
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- JP2013540449A5 JP2013540449A5 JP2013535508A JP2013535508A JP2013540449A5 JP 2013540449 A5 JP2013540449 A5 JP 2013540449A5 JP 2013535508 A JP2013535508 A JP 2013535508A JP 2013535508 A JP2013535508 A JP 2013535508A JP 2013540449 A5 JP2013540449 A5 JP 2013540449A5
- Authority
- JP
- Japan
- Prior art keywords
- probe
- tho
- case
- pco
- probes
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Links
- 239000000523 sample Substances 0.000 claims 71
- 238000002844 melting Methods 0.000 claims 25
- 230000008018 melting Effects 0.000 claims 25
- 238000003199 nucleic acid amplification method Methods 0.000 claims 18
- 230000003321 amplification Effects 0.000 claims 15
- 238000006243 chemical reaction Methods 0.000 claims 10
- 108091034117 Oligonucleotide Proteins 0.000 claims 9
- 238000000034 method Methods 0.000 claims 9
- 108020004707 nucleic acids Proteins 0.000 claims 9
- 102000039446 nucleic acids Human genes 0.000 claims 9
- 150000007523 nucleic acids Chemical class 0.000 claims 9
- 230000000295 complement effect Effects 0.000 claims 7
- 239000011541 reaction mixture Substances 0.000 claims 7
- 238000009396 hybridization Methods 0.000 claims 6
- 108700028369 Alleles Proteins 0.000 claims 4
- 125000002467 phosphate group Chemical group [H]OP(=O)(O[H])O[*] 0.000 claims 4
- 239000013615 primer Substances 0.000 claims 4
- 239000002773 nucleotide Substances 0.000 claims 3
- 125000003729 nucleotide group Chemical group 0.000 claims 3
- 102000054765 polymorphisms of proteins Human genes 0.000 claims 3
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 claims 2
- 238000000295 emission spectrum Methods 0.000 claims 2
- 239000003155 DNA primer Substances 0.000 claims 1
- 108090000790 Enzymes Proteins 0.000 claims 1
- 102000004190 Enzymes Human genes 0.000 claims 1
- 235000010469 Glycine max Nutrition 0.000 claims 1
- 244000068988 Glycine max Species 0.000 claims 1
- 101710163270 Nuclease Proteins 0.000 claims 1
- 238000004458 analytical method Methods 0.000 claims 1
- 238000003556 assay Methods 0.000 claims 1
- 230000015556 catabolic process Effects 0.000 claims 1
- 238000006731 degradation reaction Methods 0.000 claims 1
- 230000000694 effects Effects 0.000 claims 1
- 238000010348 incorporation Methods 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GBGB1017978.6A GB201017978D0 (en) | 2010-10-25 | 2010-10-25 | Multiplex amplification and detection |
| GB1017978.6 | 2010-10-25 | ||
| PCT/GB2011/052068 WO2012056227A2 (en) | 2010-10-25 | 2011-10-25 | Multiplex amplification and detection |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2013540449A JP2013540449A (ja) | 2013-11-07 |
| JP2013540449A5 true JP2013540449A5 (enExample) | 2014-12-11 |
| JP6021813B2 JP6021813B2 (ja) | 2016-11-09 |
Family
ID=43365464
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2013535508A Active JP6021813B2 (ja) | 2010-10-25 | 2011-10-25 | 多重増幅および検出 |
Country Status (5)
| Country | Link |
|---|---|
| US (2) | US9284607B2 (enExample) |
| EP (2) | EP2633072A2 (enExample) |
| JP (1) | JP6021813B2 (enExample) |
| GB (1) | GB201017978D0 (enExample) |
| WO (1) | WO2012056227A2 (enExample) |
Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB201017978D0 (en) | 2010-10-25 | 2010-12-08 | Oxitec Ltd | Multiplex amplification and detection |
| EP2880184B1 (en) | 2012-08-03 | 2021-03-31 | California Institute of Technology | Multiplexing and quantification in pcr with reduced hardware and requirements |
| EP2889381A1 (en) * | 2013-12-27 | 2015-07-01 | Universite De Liege | Detection method for species specific identification of nucleic acid of interest |
| WO2015147370A1 (en) | 2014-03-28 | 2015-10-01 | Seegene, Inc. | Detection of target nucleic acid sequences using different detection temperatures |
| CN107208139B (zh) | 2014-12-09 | 2020-11-10 | Seegene株式会社 | 与靶核酸序列有关的信号的分辨 |
| US11485998B2 (en) | 2015-12-15 | 2022-11-01 | Seegene, Inc. | Signal extraction for a target nucleic acid sequence |
| WO2017131463A1 (en) * | 2016-01-26 | 2017-08-03 | Seegene, Inc. . | Methods for providing signal for target nucleic acid sequence |
| EP3548634B1 (en) * | 2016-12-02 | 2021-02-24 | CONGEN Biotechnologie GmbH | Probe for detection of snps |
| AU2019209444A1 (en) * | 2018-01-22 | 2020-07-30 | Luminex Corporation | Methods and compositions for discrete melt analysis |
| WO2019204357A1 (en) | 2018-04-17 | 2019-10-24 | ChromaCode, Inc. | Methods and systems for multiplex analysis |
| US12203129B2 (en) | 2018-07-03 | 2025-01-21 | ChromaCode, Inc. | Formulations and signal encoding and decoding methods for massively multiplexed biochemical assays |
| WO2020031156A1 (en) | 2018-08-09 | 2020-02-13 | Speedx Pty Ltd | Multiplex detection of nucleic acids |
| CA3181184A1 (en) * | 2020-06-30 | 2020-10-15 | Speedx Pty Ltd | Multiplex detection of nucleic acids using mixed reporters |
Family Cites Families (25)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0232967B1 (en) | 1986-01-10 | 1993-04-28 | Amoco Corporation | Competitive homogeneous assay |
| US5210015A (en) | 1990-08-06 | 1993-05-11 | Hoffman-La Roche Inc. | Homogeneous assay system using the nuclease activity of a nucleic acid polymerase |
| US5925517A (en) | 1993-11-12 | 1999-07-20 | The Public Health Research Institute Of The City Of New York, Inc. | Detectably labeled dual conformation oligonucleotide probes, assays and kits |
| AU726501B2 (en) | 1996-06-04 | 2000-11-09 | University Of Utah Research Foundation | Monitoring hybridization during PCR |
| US6117635A (en) | 1996-07-16 | 2000-09-12 | Intergen Company | Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon |
| GB9812768D0 (en) | 1998-06-13 | 1998-08-12 | Zeneca Ltd | Methods |
| US6140054A (en) | 1998-09-30 | 2000-10-31 | University Of Utah Research Foundation | Multiplex genotyping using fluorescent hybridization probes |
| US6472156B1 (en) | 1999-08-30 | 2002-10-29 | The University Of Utah | Homogeneous multiplex hybridization analysis by color and Tm |
| ATE332398T1 (de) | 2000-08-11 | 2006-07-15 | Univ Utah Res Found | Einfach markierte oligonukleotidsonden |
| US7785776B2 (en) | 2002-05-13 | 2010-08-31 | Idaho Technology, Inc. | Genotyping by amplicon melting curve analysis |
| US20040022764A1 (en) | 2002-07-31 | 2004-02-05 | Hanan Polansky | Inhibition of microcompetition with a foreign polynucleotide as treatment of chronic disease |
| JP4608483B2 (ja) | 2003-02-18 | 2011-01-12 | アプライド バイオシステムズ， エルエルシー | ポリヌクレオチドの複合的分析のための組成物および方法 |
| US20050053950A1 (en) | 2003-09-08 | 2005-03-10 | Enrique Zudaire Ubani | Protocol and software for multiplex real-time PCR quantification based on the different melting temperatures of amplicons |
| JP5162133B2 (ja) * | 2003-12-03 | 2013-03-13 | アボット・ラボラトリーズ | 二本鎖直鎖核酸プローブ及びその使用 |
| US7671184B2 (en) | 2004-09-24 | 2010-03-02 | Universitact Bern | Molecular beacons |
| ATE494295T1 (de) | 2004-10-18 | 2011-01-15 | Univ Brandeis | Verfahren zur nukleinsäureamplifikation |
| AU2006204087B2 (en) | 2005-01-03 | 2010-09-16 | The Government Of The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services, Centers For Disease Control And Prevention | Primer for nucleic acid detection |
| WO2007045890A1 (en) | 2005-10-21 | 2007-04-26 | Primerdesign Ltd | Probe |
| JP2010505438A (ja) | 2006-10-09 | 2010-02-25 | オキシテック・リミテッド | 核酸配列を増幅および検出する方法 |
| PT1911852E (pt) | 2006-10-12 | 2009-10-09 | Bio Rad Pasteur | Sondas de cadeia dupla para a detecção fluorescente de ácidos nucleicos |
| GB0703997D0 (en) * | 2007-03-01 | 2007-04-11 | Oxitec Ltd | Methods for detecting nucleic sequences |
| ATE554187T1 (de) * | 2007-03-08 | 2012-05-15 | Idaho Technology | Verfahren für die schmelzanalyse der nukleinsäuren |
| US9234232B2 (en) | 2008-07-31 | 2016-01-12 | Oxitec Limited | Multiplex amplification and detection |
| GB0909333D0 (en) | 2009-06-01 | 2009-07-15 | Fu Guoliang | Multiplex amplification and detection |
| GB201017978D0 (en) | 2010-10-25 | 2010-12-08 | Oxitec Ltd | Multiplex amplification and detection |
-
2010
- 2010-10-25 GB GBGB1017978.6A patent/GB201017978D0/en not_active Ceased
-
2011
- 2011-10-25 EP EP11781583.7A patent/EP2633072A2/en not_active Withdrawn
- 2011-10-25 US US13/822,129 patent/US9284607B2/en active Active
- 2011-10-25 JP JP2013535508A patent/JP6021813B2/ja active Active
- 2011-10-25 WO PCT/GB2011/052068 patent/WO2012056227A2/en not_active Ceased
- 2011-10-25 EP EP16198104.8A patent/EP3150726B1/en active Active
-
2016
- 2016-03-14 US US15/069,114 patent/US10400276B2/en active Active
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