CN102187779A - Low-altitude cultivation method for polygonum viviparum - Google Patents
Low-altitude cultivation method for polygonum viviparum Download PDFInfo
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Abstract
The invention provides a low-altitude cultivation method for polygonum viviparum. The problem that the polygonum viviparum cannot be cultivated under low-altitude condition is solved by adopting the means of formulated fat ball and shed house temperature control. Meanwhile, the polygonum viviparum cultivated by the method has the characteristics of high glucose, high ash content and high K, and also has the characteristics that arginine serves as a main component and glutamic acid, histidine and aspartic acid serve as auxiliary components on the basis of multiple amino acids.
Description
Technical field
The present invention relates to a kind of cultivation method of polygonaceae plant, related to the low height above sea level cultivation method of a kind of serpentgrass in particular.
Technical background
Serpentgrass Polygonum viviparum L. is a polygonaceae Polygonum herbaceos perennial, grows in moist meadow, river shoal, shrub etc. the ground of height above sea level 2300~4000m, is distributed widely in ground such as each provinces and regions of China, Southeast Asia, the Central Asia, Europe and North America.Serpentgrass is widely used simply Chinese herbal medicine, have bring down a fever, antidiarrheal, menstruation regulating, anastaltic effect, the illnesss such as treatment rheumatic arthritis, dysentery, chronic hepatitis, venomous snake bite, traumatic injury, indigestion, tuberculosis, diarrhoea, irregular menstruation, uterine bleeding, leukorrhea, haematemesis, traumatism and bleeding that are mainly used among the people.But the serpentgrass rhizome is very slow because of the growth of low temperature cause in the highlands, is difficult to meet the need of market; And excavate the serpentgrass rhizome in a large number and can cause the destruction that is difficult to recover to the grassy marshland system of highlands, have a strong impact on the ecotope of this area.Low height above sea level artificial cultivation serpentgrass both can utilize the high temperature of low height above sea level to promote its root growth, and the product of artificial propagation simultaneously replace wild resource, also help preserving the ecological environment.At present, the report of also not planting about the low height above sea level of serpentgrass.
Summary of the invention
The invention provides the low height above sea level cultivation method of a kind of serpentgrass for addressing the above problem, may further comprise the steps:
1) spreading thickness is the mud particles of 3~4cm particle diameter less than 0.5cm on the wooden case seedbed;
2) humus and earth are added the fertile ball of 10ml nutrient solution mixing manufacture diameter 6cm in the ratio of 1:1;
3) then before June daily mean temperature rise to 22 ℃ ± 3 ℃, average daily soil temperature and rise to 20.5 ± 3 ℃, average relative humidity 81 ± 5% o'clock, with the bulbil tip of serpentgrass up, spheric end is planted down into fertile ball, expose the first half, place in the seedbed, the insulation of lid plastic film must be taked cooling measure when the canopy temperature is higher than 27 ℃;
4) 24 ± 2 ℃ of daily mean temperatures after June then, 23 ± 2 ℃ of average daily soil temperatures, average relative humidity 81 ± 5% o'clock, the seedling that about 2~5cm is long is with fertile ball to be transplanted in the diameter 10-20cm flowerpot, fill up soil 2~5cm in the alms bowl earlier, add the about 8~15g of dried barnyard manure, blinding 2~5cm, the fertile ball of packing into, banket around the fertile ball, expose seedling, water normal root water, Lin Yin avoids at the place sunlight direct irradiation;
5) transplant the back 10~15, impose N-P-K mixed fertilizer for the first time, after September, 23.6 ± 2 ℃ of daily mean temperatures, 23.1 ± 2 ℃ of average daily soil temperatures, average relative humidity 94.1 ± 5% o'clock imposes N-P-K mixed fertilizer for the second time, the soil moisture conservation of watering every day.
Described step 2) nutrient solution in comprises macroelement and trace element, and wherein, the content that macroelement is every liter is respectively: KNO
3350~400mg, NH
4NO
3300~350mg, KH
2PO
420~55mg, MgSO
4.7H
2O 50~100 mg, CaCl
2.2H
2O 60~100mg;
The content that trace element is every liter is: KI 0.1~0.2mg, H
3BO
31~2 mg, MnSO
4.4H
2O 3~5 mg, ZnSO
4.7H
2O 1.5~2 mg, Na
2MoO
4.2H
2O 0.02~0.06 mg, CuSO
4.5H
2O 0.001~0.006mg, CoCl
2.6H
2O 0.003~0.008mg.
N-P-K mixed fertilizer in the described step 5) is: dig pit to bury and execute urea 2g, contain K in plant soil table root far away place
2The potash fertilizer 2g of O 60%, superphosphate 8g.
Useful technique effect of the present invention is: the low height above sea level cultivation method of the described serpentgrass of the present patent application has solved the difficult problem that serpentgrass can not be cultivated under low height above sea level condition, the serpentgrass of adopting this method to cultivate simultaneously has the characteristics of high sugar, high ash content, high K, based on arginine, glutamic acid, histidine and asparatate are the characteristics of assisting on the basis of several amino acids.
Embodiment
Embodiment 1
Select height above sea level to be lower than two identical plot of 650 meters, optional one is the experiment plot, and other one is to duplicate the contrast plot of serpentgrass in high altitude localities clay fertilizer condition; Make length and be respectively 100cm, 60cm, the big boxes of 10cm in the experiment plot, spread the thick fine earth particle of about 3-4cm at the bottom of the case, the spray clear water is wetting.3 2cm are wide, and the bending of the long bamboo bar of 100cm is fixed on the wooden case top as support.
By following prescription take by weighing raw material preparation nutrient solution (unit :/L): KNO
3380mg, NH
4NO
3330 mg, KH
2PO
434mg, MgSO
4.7H
2O 74 mg, CaCl
2.2H
2O 88mg, KI 0.166 mg, H
3BO
31.24 mg, MnSO
4.4H
2O 4.46 mg, ZnSO
4.7H
2O 1.72 mg, Na
2MoO
4.2 H
2O 0.05 mg, CuSO
4.5 H
2O 0.005 mg, CoCl
2.6 H
2O 0.005mg;
Make fertile ball: in humus soil: normal soil ratio 1:1, mix, Ensure Liquid liquid 10ml makees the fertile ball of diameter 6cm.
Late May (22 ℃ of daily mean temperatures, 20.5 ℃ of average daily soil temperatures, the average relative humidity 81%) growing way of gathering stalwartness, the full bulbil of profile, the bulbil tip up, spheric end is planted down into fertile ball, exposes the first half; Neatly place in turn on the booth seedbed, the gap is spread fine earth and is filled up spray normal root water between fertile ball.Cover transparent plastic film on the support of seedbed, film four limits are pushed down with brick.In the time of temperature of shed 27-30 ℃, open the film radiate heat and ventilate;
Late June (24.3 ℃ of daily mean temperatures, 23.6 ℃ of average daily soil temperatures, average relative humidity 81.3%), be transplanted into the little flowerpot of the big plastics of diameter 15cm with fertile ball when the about 2-5cm of seedling is long, the about 4cm of pad soil is thick earlier in the flowerpot, add the about 10g of dried barnyard manure, blinding 4cm is thick, reinstalls fertile ball, bankets around the fertile ball, expose seedling, water normal root water; Place Lin Yin to cultivate down;
Transplant (27.5 ℃ of daily mean temperatures, 26.8 ℃ of average daily soil temperatures, average relative humidity 83.3% after 15-20 days.), about 90% transplanted seedling grows young leaves, shows to survive, and uses and topdresses: urea 2g/ strain, potash fertilizer (contains K
2O 60%) 2 g/ strains, superphosphate 8g/ strain.Dig a hole and imbed in Yu Tubiao root far away place; Look the soil moisture conservation of watering of soil moisture situation.Especially at late July-mid-August (33.5 ℃ of daily mean temperatures, 31.5 ℃ of average daily soil temperatures, average relative humidity 54.1%.) during this period of time, the temperature height, air humidity is little, the soil moisture conservation of watering every day; Early September, (23.6 ℃ of daily mean temperatures, daily soil temperature was 23.1 ℃, average relative humidity 94.1%.) topdress 1 time: urea 2g/ strain, potash fertilizer (contains K
2O 60%) 2 g/ strains, superphosphate 8 g/ strains, loose stool water is a small amount of.
Get thin river sand in contrast in the plot, choose impurity, clean up, dry, the long 30cm that packs into, wide 20cm, high 5cm, the iron pan that the bottom has aperture to leak, the spray proper amount of clear water, all wetting with river sand, hand is pinched river sand and do not dripped and be advisable, and is standby;
Late May, (22 ℃ of daily mean temperatures, daily soil temperature was 20.5 ℃, average relative humidity 81%.) growing way of gathering stalwartness, the full bulbil of profile, the bulbil tip up, spheric end is planted in the wet river sand down, exposes the first half, sprays a small amount of normal root water, cultivates in open country;
Late June, (24.3 ℃ of daily mean temperatures, daily soil temperature was 23.6 ℃, average relative humidity 81.3%.), the suitable quantity of water of falling is earlier gone into sand table, makes river sand soft, prize from sand with tweezers and to get the long seedling of 2-5cm, clean the root river sand in clear water, be transplanted into the little flowerpot of the big plastics of diameter 15cm, the about 4cm of pad soil is thick earlier in the flowerpot, add the about 10g of dried barnyard manure, blinding 4cm is thick, is transplanted into seedling again, notes keeping root to stretch, water normal root water, cultivate in open country;
Transplant (27.5 ℃ of daily mean temperatures, 26.8 ℃ of average daily soil temperatures, average relative humidity 83.3% after 15-20 days.), about 90% transplanted seedling grows young leaves, shows to survive, and uses and topdresses: urea 2g/ strain, potash fertilizer (contains K
2O 60%) 2 g/ strains, superphosphate 8 g/ strains; Dig a hole and imbed in Yu Tubiao root far away place; Look the soil moisture conservation of watering of soil moisture situation; Especially at late July-mid-August (33.5 ℃ of daily mean temperatures, 31.5 ℃ of average daily soil temperatures, average relative humidity 54.1%.) during this period of time, the temperature height, air humidity is little, and the soil moisture conservation of watering every day covers the sunshade net, and cover when 10:00-4:00 in afternoon temperature is high the morning, and all the other times open.
The germination rate in statistical experiment plot and contrast plot respectively, surviving of plot of experiment is 42.35%, the contrast plot survival rate be 0.
Embodiment 2
Adopt the sampling of multidraw method.The serpentgrass of adopting embodiment 1 described low height above sea level experiment plot to gather is first group; The serpentgrass of picking up from the black fascine dam of Wuxi County, Chongqing City height above sea level 1 650m is second group; The serpentgrass that the conch ditch that picks up from Luding County, state, Ganzi height above sea level 3500m is produced in Sichuan is the 3rd group.Sample remove foreign material and with running water silt is rinsed well after dry in the shade, pulverize, mistake 50 mesh sieves, standby.
Assay method is respectively: moisture: gravimetric method; Ash content: calcination mass method; Crude fat: Soxhlet extraction process; Crude protein: use guild gram Kai Shi system of Foster Kato company to carry out the kjeldahl determination analysis; Total reducing sugar: film reagent method; Amino acid: adopt the L-8800 of Hitachi automatic amino acid analyzer to measure; Mineral element: adopt the TRS-990 atomic absorption spectrophotometer.Every duplicate samples repeats 3 times and measures, and averages.Concrete outcome sees Table 1-table 3;
Table 1 main nutrient composition and content
Table 1 is the result show, the total sugar content in low height above sea level experiment plot is significantly higher than the Sichuan sample and the Chongqing sample is approaching, and in 5 projects that detect, the ash content of Chongqing sample and total sugar content are significantly higher than the Sichuan sample; The moisture and the content of crude fat in low height above sea level experiment plot will be higher than the Chongqing sample.
Table 2 essential mineral prime element kind and content are relatively
As seen from Table 2, the serpentgrass rhizome in three places of production all detects P, K, 4 kinds of macroelement of Ca, Mg and Fe, Mn, Zn, Cu, 5 kinds of trace elements of B.In the constituent content ordering, the content of serpentgrass main mineral elements order from high to low is K〉Ca〉Mg〉Fe〉P〉Mn〉B〉Zn〉Cu; The content order from high to low that the serpentgrass main mineral elements is produced in low height above sea level experiment plot is K〉Ca〉Mg〉Fe〉P〉Mn〉B〉Zn〉Cu; The content order from high to low that the serpentgrass main mineral elements is produced in Sichuan is Mg〉K〉Ca〉Fe〉P〉Mn〉B〉Zn〉Cu, low height above sea level experiment plot serpentgrass except that the content ordering of Mg, K and three kinds of elements of Ca in two places of production variant, the content ordering of all the other elements is identical.Wherein the content difference of K, Mg, Fe and 4 kinds of elements of Mn reaches significance level (P<0.05), and the difference of all the other 5 kinds of constituent contents is remarkable (P ﹤ 0.05) not.
Main amino acid kind of table 3 and content are relatively
As seen from Table 3, three samples all detect l7 kind hydrolysis amino acid and l6 kind free amino acid.Hydrolysis and free amino acid total amount that serpentgrass is produced in low height above sea level experiment plot are 18.438mg/g, there is not significant difference (P〉0.05) with the total amino acid content of Sichuan and Chongqing sample, wherein the total amount of hydrolysis and free arginine is close at two place of production samples, and is significantly higher than the total amount of other hydrolysis and free amino acid in the sample of the corresponding place of production.The total amount difference of all the other various hydrolysis and free amino acid is (P〉O.05) not significantly.
The hydrolysis amino acid total amount that serpentgrass is produced in low height above sea level experiment plot is 17441mg/g, be significantly higher than the hydrolysis amino acid total amount (P<0.05) of Sichuan sample, wherein the content of 9 seed amino acids such as hydrolysis arginine, glutamic acid, histidine, lysine, tyrosine, valine, phenyl alanine, cystine and methionine will be higher than the Sichuan sample, except that the content difference of hydrolysis arginine, histidine and methionine is remarkable (P<O.05), all the other hydrolysis amino acid total amount differences are remarkable (P ﹥ 0.05) not.The content of 8 seed amino acids such as the amino acid of hydrolysis asparagus, glycine, serine, leucine, threonine, alanine, proline and isoleucine that the Sichuan sample is contained will be higher than low height above sea level experiment plot and produce serpentgrass, but difference is all not remarkable.In sum, low height above sea level experiment plot is produced serpentgrass and is had the characteristics of high sugar, high ash content, high K, and based on arginine, glutamic acid, histidine and asparatate are the nutrient component characteristics of assisting on the basis of several amino acids.
Claims (3)
1. a serpentgrass hangs down the height above sea level cultivation method, it is characterized in that: may further comprise the steps:
1) spreading thickness is the mud particles of 3~4cm particle diameter less than 0.5cm on the wooden case seedbed;
2) humus and earth are added the fertile ball of 10ml nutrient solution mixing manufacture diameter 6cm in the ratio of 1:1;
3) then before June daily mean temperature rise to 22 ℃ ± 3 ℃, average daily soil temperature and rise to 20.5 ± 3 ℃, average relative humidity 81 ± 5% o'clock, with the bulbil tip of serpentgrass up, spheric end is planted down into fertile ball, expose the first half, place in the seedbed, the insulation of lid plastic film must be taked cooling measure when the canopy temperature is higher than 27 ℃;
4) 24 ± 2 ℃ of daily mean temperatures after June then, 23 ± 2 ℃ of average daily soil temperatures, average relative humidity 81 ± 5% o'clock, the seedling that about 2~5cm is long is with fertile ball to be transplanted in the diameter 10-20cm flowerpot, fill up soil 2~5cm in the alms bowl earlier, add the about 8~15g of dried barnyard manure, blinding 2~5cm, the fertile ball of packing into, banket around the fertile ball, expose seedling, water normal root water, Lin Yin avoids at the place sunlight direct irradiation;
5) transplant the back 10~15, impose N-P-K mixed fertilizer for the first time, after September, 23.6 ± 2 ℃ of daily mean temperatures, 23.1 ± 2 ℃ of average daily soil temperatures, average relative humidity 94.1 ± 5% o'clock imposes N-P-K mixed fertilizer for the second time, the soil moisture conservation of watering every day.
2. serpentgrass according to claim 1 hangs down the height above sea level cultivation method, and it is characterized in that: the nutrient solution described step 2) comprises macroelement and trace element, and wherein, the content that macroelement is every liter is respectively: KNO
3350~400mg, NH
4NO
3300~350mg, KH
2PO
420~55mg, MgSO
4.7H
2O 50~100 mg, CaCl
2.2H
2O 60~100mg;
The content that trace element is every liter is: KI 0.1~0.2mg, H
3BO
31~2 mg, MnSO
4.4H
2O 3~5 mg, ZnSO
4.7H
2O 1.5~2 mg, Na
2MoO
4.2H
2O 0.02~0.06 mg, CuSO
4.5H
2O 0.001~0.006mg, CoCl
2.6H
2O 0.003~0.008mg.
3. serpentgrass according to claim 1 hangs down the height above sea level cultivation method, and it is characterized in that: the N-P-K mixed fertilizer in the described step 5) is: dig pit to bury and execute urea 2g, contain K in plant soil table root far away place
2The potash fertilizer 2g of O 60%, superphosphate 8g.
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Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
RU2497867C1 (en) * | 2012-07-10 | 2013-11-10 | Федеральное государственное бюджетное образовательное учреждение высшего профессионального образования "Горский государственный аграрный университет" | Substrate for planting seedlings of fruit crops |
CN105192003A (en) * | 2015-10-19 | 2015-12-30 | 广东省昆虫研究所 | Application of polygonum viviparum root in preparing insecticide for plutella xylostella and spodoptera litura fabricius |
CN109734498A (en) * | 2019-03-04 | 2019-05-10 | 西南大学 | A kind of serpentgrass water planting nutrient solution and serpentgrass ciltivating process |
CN110012827A (en) * | 2019-03-20 | 2019-07-16 | 广东东阳光药业有限公司 | A kind of serpentgrass method for culturing seedlings |
CN112119894A (en) * | 2020-08-13 | 2020-12-25 | 神农架祥玺农业科技有限公司 | Seedling growing method of polygonum bulbiferum in plastic greenhouse |
CN112219678A (en) * | 2020-11-12 | 2021-01-15 | 东莞市东阳光冬虫夏草研发有限公司 | Polygonum criopolitanum planting matrix and method for promoting growth of polygonum criollis |
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CN1481666A (en) * | 2002-09-12 | 2004-03-17 | 贵州威门药业股份有限公司 | Method for planting polygonum capilalum |
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2011
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CN1481666A (en) * | 2002-09-12 | 2004-03-17 | 贵州威门药业股份有限公司 | Method for planting polygonum capilalum |
Non-Patent Citations (2)
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《中国优秀硕士学位论文全文数据库》 20100615 邹园 珠芽蓼的开花结实与胎生繁殖 摘要 1-3 , 第6期 2 * |
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Cited By (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
RU2497867C1 (en) * | 2012-07-10 | 2013-11-10 | Федеральное государственное бюджетное образовательное учреждение высшего профессионального образования "Горский государственный аграрный университет" | Substrate for planting seedlings of fruit crops |
CN105192003A (en) * | 2015-10-19 | 2015-12-30 | 广东省昆虫研究所 | Application of polygonum viviparum root in preparing insecticide for plutella xylostella and spodoptera litura fabricius |
CN105192003B (en) * | 2015-10-19 | 2017-11-21 | 广东省生物资源应用研究所 | Serpentgrass root is preparing diamondback moth and the application in prodenia litura insecticide |
CN109734498A (en) * | 2019-03-04 | 2019-05-10 | 西南大学 | A kind of serpentgrass water planting nutrient solution and serpentgrass ciltivating process |
CN110012827A (en) * | 2019-03-20 | 2019-07-16 | 广东东阳光药业有限公司 | A kind of serpentgrass method for culturing seedlings |
CN112119894A (en) * | 2020-08-13 | 2020-12-25 | 神农架祥玺农业科技有限公司 | Seedling growing method of polygonum bulbiferum in plastic greenhouse |
CN112219678A (en) * | 2020-11-12 | 2021-01-15 | 东莞市东阳光冬虫夏草研发有限公司 | Polygonum criopolitanum planting matrix and method for promoting growth of polygonum criollis |
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