CN105192003A - Application of polygonum viviparum root in preparing insecticide for plutella xylostella and spodoptera litura fabricius - Google Patents
Application of polygonum viviparum root in preparing insecticide for plutella xylostella and spodoptera litura fabricius Download PDFInfo
- Publication number
- CN105192003A CN105192003A CN201510680995.5A CN201510680995A CN105192003A CN 105192003 A CN105192003 A CN 105192003A CN 201510680995 A CN201510680995 A CN 201510680995A CN 105192003 A CN105192003 A CN 105192003A
- Authority
- CN
- China
- Prior art keywords
- serpentgrass
- prodenia litura
- diamond
- root
- back moth
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Feed For Specific Animals (AREA)
- Fodder In General (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention discloses application of polygonum viviparum root in preparing insecticide for plutella xylostella and spodoptera litura fabricius. Experiments prove that the polygonum viviparum root has relatively high growth inhibitory activity and toxic activity for first-fourth instar larvae of plutella xylostella and spodoptera litura fabricius, so that the polygonum viviparum root can be applied to the preparation of insecticide for plutella xylostella and spodoptera litura fabricius, and a new resource is provided for development of plant source insecticide.
Description
Technical field:
The invention belongs to pesticide field, be specifically related to the application of serpentgrass root in preparation diamond-back moth and prodenia litura insecticide.
Background technology:
Diamond-back moth Plutellaxylostella (L.), prodenia litura SpodopteralituraFabricius are the important pests of worldwide brassicaceous vegetable, fertility is strong, and generation overlap is serious, and pesticide resistance is strong, difficulty of prevention and cure high (Ahmadetal., 2008; Feng Xia etc., 2011; Zhangetal., 2015).
Serpentgrass (PolygonumviviparumL.) is polygonaceae Polygonum perennial herb, distribution is all had in China Tibet, Qinghai, Jilin, the Inner Mongol, Shaanxi, Gansu, Sichuan etc., primary growth is in the damp area of the alpine steppe of height above sea level 2220 ~ 5100m (Li Youzhong etc., 1996).Serpentgrass is not only traditional Tibetan medicine, herbage and liquor-making raw material, main foodstuff (Li Youzhong etc., 1996 of its rhizome Ye Shi China rare medicinal material host of Cordyceps sinensis insect; Wang Junli etc., 2010; Weng Hua, 2011; Shandong is added lustre, and 2013).
Summary of the invention:
The object of this invention is to provide the application of serpentgrass root in preparation diamond-back moth or prodenia litura insecticide.
The present invention to feed diamond-back moth and prodenia litura respectively at the fresh serpentgrass root powder adding 5%, 15%, 25% in the artificial feed of diamond-back moth and prodenia litura, determine the impact of fresh serpentgrass root on diamond-back moth and Development of Spodoptera litura F, result shows, the 1-4 larva of serpentgrass root to diamond-back moth and prodenia litura has very strong growth inhibitory activity and cytotoxicity, has obvious cytotoxicity to prodenia litura 5 instar larvae.
Therefore, serpentgrass root can be applied in preparation diamond-back moth or prodenia litura insecticide.
Described insecticide is preferably stomach insecticide.
Another object of the present invention is to provide a kind of diamond-back moth or prodenia litura insecticide, it is characterized in that, comprises diamond-back moth or prodenia litura feed, and serpentgrass root powder, and the content of described serpentgrass root powder is mass fraction 5 ~ 25%.
The present invention is confirmed by test, the 1-4 larva of serpentgrass root to diamond-back moth and prodenia litura has very strong growth inhibitory activity and cytotoxicity, to prodenia litura 5 instar larvae, there is obvious cytotoxicity, therefore serpentgrass root can application in preparation diamond-back moth or prodenia litura insecticide, thus provides new resources and path for the exploitation of plant insecticide.
Accompanying drawing illustrates:
The percentage that Fig. 1 is diamond-back moth 1 instar larvae feeding 3d (A), 8d (B) respectively grows the length of time afterwards, note: the different letter representations significant difference to each other on identical column-shape strip figure;
Fig. 2 is the percentage that diamond-back moth 2 instar larvae feeding 3d (A) and 9d (B) respectively grow the length of time afterwards, note: the different letter representations significant difference to each other on identical column-shape strip figure;
Fig. 3 is the percentage that diamond-back moth 3 instar larvae feeding 3d (A) and 7d (B) respectively grow the length of time afterwards, note: the different letter representations significant difference to each other on identical column-shape strip figure;
Fig. 4 is the percentage that diamond-back moth 4 instar larvae feeding 3d (A) and 6d (B) respectively grow the length of time afterwards, note: the different letter representations significant difference to each other on identical column-shape strip figure;
The percentage that Fig. 5 is prodenia litura 1 instar larvae feeding 9d (A), 12d (B) and 20d (C) respectively grows the length of time afterwards, note: the different letter representations significant difference to each other on identical column-shape strip figure;
The percentage that Fig. 6 is prodenia litura 2 instar larvae feeding 9d (A), 12d (B) and 20d (C) respectively grows the length of time afterwards, note: the different letter representations significant difference to each other on identical column-shape strip figure;
The percentage that Fig. 7 is prodenia litura 3 instar larvae feeding 8d (A), 12d (B) and 20d (C) respectively grows the length of time afterwards, note: the different letter representations significant difference to each other on identical column-shape strip figure;
The percentage that Fig. 8 is prodenia litura 4 instar larvae feeding 4d (A), 12d (B), 28d (C) and 34d (D) respectively grow the length of time afterwards, note: the different letter representations significant difference to each other on identical column-shape strip figure;
The percentage that Fig. 9 is prodenia litura 5 instar larvae feeding 5d (A), 12d (B) and 28d (C) respectively grows the length of time afterwards, note: the different letter representations significant difference to each other on identical column-shape strip figure;
The percentage that Figure 10 is prodenia litura 6 instar larvae feeding 3d (A), 8d (B), 20d (C) and 24d (D) respectively grow the length of time afterwards, note: the different letter representations significant difference to each other on identical column-shape strip figure.
Embodiment:
Following examples further illustrate of the present invention, instead of limitation of the present invention.
Embodiment 1:
1.1 for examination insect
Eggs of Diamondback Moth (P.xylostella) is provided by Institute of Plant Protection of academy of agricultural sciences of Guangdong Province, and prodenia litura (S.litura) ovum provided by Zhongshan University Life Science College insect.After the egg hatching of two kinds of insects, get newly hatched larvae as 1 instar larvae for administering transgenic, diamond-back moth newly hatched larvae is raised with diamond-back moth artificial feed and is used for administering transgenic to 2,3,4 instar larvaes, and prodenia litura newly hatched larvae is raised to 2,3,4,5,6 instar larvaes for administering transgenic with prodenia litura artificial feed.
1.2 test plant
Serpentgrass root (P.viviparum) gathers from kangding, with aseptic water washing at least 3 times after clean with clean water, be placed in after super-clean bench dries up, package, 4 DEG C of preservations.Before preparation artificial feed, remove the coring of serpentgrass root, cut fritter, then smashing with meat grinder becomes powder.
1.3 artificial feed
The artificial feed of diamond-back moth are purchased from Institute of Plant Protection of academy of agricultural sciences of Guangdong Province, and the artificial feed of prodenia litura are purchased from Life Science College insect institute of Zhongshan University.
1.4.1 the mensuration of feeding of diamond-back moth
1.4 feed mensuration
The serpentgrass root powder of 5%, 15%, 25% (w/w) content is added respectively in the artificial feed of diamond-back moth, abundant mixing, be prepared into the feed that serpentgrass root powder content is 5%, 15%, 25%, do not add the artificial feed of serpentgrass root powder in contrast.Get the culture dish (diameter 9cm) being lined with 2 metafiltration paper, add feed 1 fritter toward culture dish, every ware accesses 20 diamondback moth larvaes, often processes repetition 3 times, often processes total larva number 60.Culture dish is placed in the climate box that temperature is 25 DEG C, relative moisture is 70%, light application time is 14:10 (L:D) to cultivate.Observe every day with record the taking food of larva, grow the situation such as the length of time, death, within 2-3 days, add or replacing feed.
1.4.2 the mensuration of feeding of prodenia litura
The serpentgrass root powder of 5%, 15%, 25% (w/w) content is added respectively in the artificial feed of prodenia litura, abundant mixing, be prepared into the feed that serpentgrass root powder content is 5%, 15%, 25%, do not add the artificial feed of serpentgrass root powder (0%) in contrast.Get the plastic casing being lined with 2 metafiltration paper, add feed 1-4 block toward plastic casing, add the size of amount depending on larva of feed, every ware accesses 20 Spodoptera litura larvae, often processes repetition 3 times, often processes total larva number 60.Culture dish is placed in the climate box that temperature is 25 DEG C, relative moisture is 70%, light application time is 14:10 (L:D) to cultivate.Observe every day with record the taking food of larva, grow the situation such as the length of time, death, every day adds feed.
1.5 data analysis
The each budding ratio of insect, lethality percentage represent, percentage is through evolution, arcsine transformation process.Carry out one-way analysis of variance with SPSS16.0 software, Multiple range test adopts Duncan inspection, significance level P<0.05.
2 results and analysis
The impact that 2.1 serpentgrass roots grow on diamondback moth larvae
2.1.1 serpentgrass root impact that diamond-back moth 1 instar larvae is grown
Diamond-back moth 1 instar larvae is fed after 3d, and the diamond-back moth 8.33% of not adding in the artificial feed group (control group) of serpentgrass root powder is developed to 2 instar larvaes, and 83.33% is developed to 3 instar larvaes, 8.33% death (Figure 1A); And add in 3 processed group of 5%, 15%, 25% serpentgrass root powder, diamond-back moth 2 instar larvae ratio is respectively 48.33%, 60.00%, 48.33%, 3 instar larvae ratios are respectively 6.67%, 5.00%, 1.67%, and lethality is respectively up to being 45.00%, 35.00%, 50.00% (Figure 1A).Feed after 8d, in control group, the diamond-back moth of 78.33% is developed to 4 instar larvaes, and 8.3.% is pupated, and lethality only has 13.33%; And the diamond-back moth lethality of adding in the processed group of 5%, 15%, 25% serpentgrass root powder is respectively up to 93.33%, 93.33%, 88.33%, do not have diamond-back moth can be developed to 4 instar larvaes (Figure 1B).Feed after 14d, in control group, the diamond-back moth of 81.67% sprouts wings, and lethality only has 15.00%; And the diamond-back moth 100% of adding in 5%, 15%, 25% serpentgrass root powder processed group is dead.Result shows, the serpentgrass root powder that feed adds 5%, 15%, 25% is fed diamond-back moth 1 instar larvae, developmental rate is significantly lower than control group, lethality is significantly higher than contrast, proportional difference in each length of time at developmental stage between 3 processed group of adding serpentgrass root powder is not obvious, and diamond-back moth all fails to be developed to 4 length of times and all dead.
2.1.2 serpentgrass root impact that diamond-back moth 2 instar larvae is grown
Diamond-back moth 2 instar larvae is fed after 3d, and the diamond-back moth in control group 88.99% is developed to 3 instar larvaes, and 6.67% is dead; And add in 3 processed group of 5%, 15%, 25% serpentgrass root powder, diamond-back moth 2 instar larvae ratio is respectively 15.00%, 13.33%, 6.67%, 3 instar larvae ratios are respectively 25.00%, 20.00%, 33.33%, and lethality is respectively 58.33%, 66.67%, 60.00% (Fig. 2 A).Feed after 9d, in control group, the diamond-back moth of 77.75% pupates, and lethality only has 14.78%; And the 3 instar larvae ratios of adding in the processed group of 5%, 15%, 25% serpentgrass root powder are respectively 1.67%, 1.67%, 3.33%, lethality is respectively 98.33%, 98.33%, 96.67% (Fig. 2 B).Feed after 13d, in control group, the diamond-back moth of 79.42% sprouts wings, and lethality only has 20.58%; And the diamond-back moth 100% of adding in 5%, 15%, 25% serpentgrass root powder processed group is dead.Result shows, the serpentgrass root powder that feed adds 5%, 15%, 25% is fed diamond-back moth 2 instar larvae, developmental rate is significantly lower than control group, lethality is significantly higher than contrast, proportional difference in each length of time at developmental stage between 3 processed group of adding serpentgrass root powder is not obvious, and diamond-back moth all dies from larval phase.
2.1.3 serpentgrass root impact that diamond-back moth 3 instar larvae is grown
Diamond-back moth 3 instar larvae is fed after 3d, and the diamond-back moth in control group 100% is developed to 4 instar larvaes; And add in 3 processed group of 5%, 15%, 25% serpentgrass root powder, 3 instar larvae ratios are respectively 95.00%, 98.33%, 100%, and lethality is respectively 5.00%, 2.89%, 0.00% (Fig. 3 A).Feed after 7d, in control group, the diamond-back moth of 100% pupates; And the 3 instar larvae ratios of adding in the processed group of 5%, 15%, 25% serpentgrass root powder are respectively 3.33%, 6.67%, 8.33%, 4 instar larvae ratios are respectively 8.33%, 8.33%, 11.67%, and lethality is respectively 88.33%, 85.00%, 80.00% (Fig. 3 B).Feed after 11d, in control group, the diamond-back moth of 96.67% sprouts wings, and lethality is 3.33%; And the diamond-back moth 100% of adding in 5%, 15%, 25% serpentgrass root powder processed group is dead.Result shows, the serpentgrass root powder that feed adds 5%, 15%, 25% is fed diamond-back moth 3 instar larvae, developmental rate is significantly lower than control group, lethality is significantly higher than contrast, proportional difference in each length of time at developmental stage between 3 processed group of adding serpentgrass root powder is not obvious, and diamond-back moth all dies from larval phase.
2.1.4 serpentgrass root impact that diamond-back moth 4 instar larvae is grown
Diamond-back moth 4 instar larvae is fed after 3d, and the diamond-back moth 71.67% in control group to be developed in 4 ages larva in latter stage, and 15% pupates, and lethality is 13.33%; And add in 3 processed group of 5%, 15%, 25% serpentgrass root powder, 4 age Initial instar larvae ratio be respectively 16.67%, 18.33%, 18.33%, in 4 ages latter stage larva ratio be respectively 30.00%, 26.67%, 20.00%, lethality is respectively 50.00%, 56.67%, 61.67% (Fig. 4 A).Feed after 6d, in control group, the diamond-back moth of 63.33% pupates, and the ratio that the larva processing larva in latter stage in 4 ages accounts for is 11.67%, and lethality is 25%; And the ratio of adding diamond-back moth 4 larva in latter stage in age in the processed group of 5%, 15%, 25% serpentgrass root powder is respectively 11.67%, 3.33%, 0%, lethality is respectively up to being 88.33%, 96.67%, 100% (Fig. 4 B).Feed after 9d, in control group, the diamond-back moth of 71.67% sprouts wings, and lethality is 28.33%; And the diamond-back moth 100% of adding in 5%, 15%, 25% serpentgrass root powder processed group is dead.Result shows, the serpentgrass root powder that feed adds 5%, 15%, 25% is fed diamond-back moth 4 instar larvae, developmental rate is significantly lower than control group, lethality is significantly higher than contrast, proportional difference in each length of time at developmental stage between 3 processed group of adding serpentgrass root powder is not obvious, and diamond-back moth all dies from larval phase.
The impact that 2.2 serpentgrass roots grow on Spodoptera litura larvae
2.2.1 serpentgrass root impact that prodenia litura 1 instar larvae is grown
Prodenia litura 1 instar larvae is fed after 9d, does not add in the control group of serpentgrass root powder, and the prodenia litura of 91.67% is developed to 5 instar larvaes, and lethality is 3.33%; And the prodenia litura 2 instar larvae ratio of adding in 3 process of 5%, 15%, 25% serpentgrass root powder is respectively 76.67%, 75.00%, 56.67%, lethality is respectively 23.33%, 25.00%, 43.33%, does not have prodenia litura to be developed to 3 length of times (Fig. 5 A).Feed after 12d, in control group, the prodenia litura of 93.33% is developed to 6 instar larvaes, and lethality is 6.67%; And the prodenia litura 3 instar larvae ratio of adding in 3 process of 5%, 15%, 25% serpentgrass root powder is respectively 46.67%, 40.00%, 28.33%, lethality is respectively 53.33%, 60.00%, 71.67% (Fig. 5 B).Feed after 20d, in control group, the prodenia litura of 20% is developed to 6 instar larvaes, and the prodenia litura of 66.67% pupates, lethality 13.33%; And equal 100% death of prodenia litura (Fig. 5 C) added in 3 process of 5%, 15%, 25% serpentgrass root powder.Prodenia litura in control group to be fed after 24d and 86.67% to pupate, and after the 32d that feeds, accumulative eclosion rate is 86.67%, and cumulative mortality is 13.33%.Result shows, and the serpentgrass root powder that feed adds 5%, 15%, 25% is fed prodenia litura 1 instar larvae, and developmental rate is significantly lower than control group, and lethality is significantly higher than contrast, and prodenia litura is larva below 4 ages all, does not have prodenia litura to be developed to for 4 length of times.
The impact that 4.2 serpentgrass roots grow on prodenia litura 2 instar larvae
Prodenia litura 2 instar larvae is fed after 9d, does not add in the control group of serpentgrass root powder, and the prodenia litura of 100% is developed to 5 instar larvaes; And the prodenia litura 3 instar larvae ratio of adding in 3 of 5%, 15%, 25% serpentgrass root powder process to be respectively be 90.00%, 96.67%, 96.67%, lethality is respectively 10.00%, 3.33%, 3.33% (Fig. 6 A).Feed after 12d, in control group, the prodenia litura of 100% is developed to 6 instar larvaes; And the prodenia litura 3 instar larvae ratio of adding in 3 process of 5%, 15%, 25% serpentgrass root powder is respectively 23.33%, 21.67%, 25%, lethality is respectively 76.67%, 78.33%, 75% (Fig. 6 B).Feed after 20d, in control group, the prodenia litura of 91.67% is developed to pupa, lethality 3.33%; And equal 100% death of prodenia litura (Fig. 6 C) added in 3 process of 5%, 15%, 25% serpentgrass root powder.Prodenia litura in control group is fed after 32d, and accumulative eclosion rate is 91.67%, and cumulative mortality is 3.33%.Result shows, and the serpentgrass root powder that feed adds 5%, 15%, 25% is fed prodenia litura 2 instar larvae, and developmental rate is significantly lower than control group, and lethality is significantly higher than contrast, and prodenia litura all dies from larva in 4 ages below, does not have prodenia litura to be developed to for 4 length of times.
2.2.3 serpentgrass root impact that prodenia litura 3 instar larvae is grown
Prodenia litura 3 instar larvae is fed after 8d, does not add in the control group of serpentgrass root powder, and the prodenia litura of 15% is developed to 5 instar larvaes, and the prodenia litura of 83.33% is developed to 6 instar larvaes, and lethality is 1.67%; And the prodenia litura 4 instar larvae ratio of adding in 3 process of 5%, 15%, 25% serpentgrass root powder is respectively 6.67%, 10%, 15%, 5 instar larvae ratios are respectively 56.67%, 70%, 73.33%, lethality is respectively 36.67%, 20%, 13.33%, does not have prodenia litura can be developed to 6 length of times (Fig. 7 A).
Feed after 12d, in control group, the prodenia litura of 95% is developed to 6 instar larvaes, and lethality only has 1.67%; And the prodenia litura 5 instar larvae ratio of adding in 3 process of 5%, 15%, 25% serpentgrass root powder is respectively 46.67%, 61.67%, 78.33%, lethality is respectively 53.33%, 38.33%, 21.67%, does not have prodenia litura to be developed to 6 length of times (Fig. 7 B).Feed after 20d, in control group, the prodenia litura of 91.67% is developed to pupa, lethality 6.67%; And the prodenia litura 5 instar larvae ratio of adding in 3 process of 5%, 15%, 25% serpentgrass root powder is respectively 5%, 6.67%, 6.67%, 6 age primary larva ratio be respectively 23.33%, 36.67%, 55%, lethality is respectively 71.67%, 56.67%, 38.33% (Fig. 7 C).Fed after 30d, it is 88.33% that the prodenia litura in control group adds up eclosion rate, and cumulative mortality is 11.67%; And the prodenia litura equal 100% of adding in 3 process of 5%, 15%, 25% serpentgrass root powder is dead.Result shows, and the serpentgrass root powder that feed adds 5%, 15%, 25% is fed prodenia litura 3 instar larvae, and developmental rate is significantly lower than control group, and lethality is significantly higher than contrast, and prodenia litura, all in dying from larval phase, does not have prodenia litura to be developed to pupa time.
2.2.4 serpentgrass root impact that prodenia litura 4 instar larvae is grown
Prodenia litura 4 instar larvae is fed after 4d, does not add in the control group of serpentgrass root powder, and the prodenia litura of 46.67% is developed to 5 instar larvaes, and the prodenia litura of 53.33% is developed to 6 instar larvaes; And prodenia litura 5 instar larvae ratio 96.67%, 95%, 96.67%, the 6 instar larvae ratios that are respectively of adding in 3 process of 5%, 15%, 25% serpentgrass root powder are respectively 3.33%, 5%, 3.33% (Fig. 8 A).Feed after 12d, in control group, the prodenia litura of 13.33% is developed to 6 instar larvaes, and 83.33% prodenia litura pupates, lethality 3.33%; And the prodenia litura 6 instar larvae ratio of adding in 3 process of 5%, 15%, 25% serpentgrass root powder is respectively 63.33%, 90%, 91.67%, lethality is respectively 35%, 10%, 8.33% (Fig. 8 B).Feed after 28d, in control group, the prodenia litura of 86.67% sprouts wings, and lethality only has 13.33%; And the prodenia litura adult of adding in 3 process of 5%, 15%, 25% serpentgrass root powder is respectively 13.33%, 3.33%, 6.67%, lethality is
46.67%, 56.67%, 55% (Fig. 8 C).Fed after 34d, the prodenia litura adult ratio of adding in 3 process of 5%, 15%, 25% serpentgrass root powder is respectively 35.00%, 30.00%, 26.67%, is starkly lower than control group; Lethality is 65.00%, 70.00%, 73.33% (Fig. 8 D).Result shows, and the serpentgrass root powder that feed adds 5%, 15%, 25% is fed prodenia litura 4 instar larvae, and developmental rate is significantly lower than control group, and lethality is significantly higher than contrast.
2.2.5 serpentgrass root impact that prodenia litura 5 instar larvae is grown
Prodenia litura 5 instar larvae is fed after 4d, does not add in the control group of serpentgrass root powder, and the prodenia litura of 98.33% is developed to 6 instar larvaes, and lethality only has 1.67%; And the prodenia litura of adding the prodenia litura 71.67%, 76.67%, 85.00% in 3 process of 5%, 15%, 25% serpentgrass root powder is developed to 6 instar larvaes, lethality is 28.33%, 23.33%, 15.00% (Fig. 9 A) respectively.Feed after 12d, in control group, the prodenia litura of prodenia litura 91.67% of 13.33% pupates, and lethality only has 6.67%; And the prodenia litura of adding the prodenia litura 68.33%, 68.33%, 73.33% in 3 process of 5%, 15%, 25% serpentgrass root powder pupates, lethality is increased to 31.67%, 31.67%, 26.67% (Fig. 9 B) respectively.Feed after 28d, in control group, the prodenia litura of 86.67% sprouts wings, and lethality only has 13.33%; And the prodenia litura emergence ratio of adding in 3 process of 5%, 15%, 25% serpentgrass root powder is respectively 61.67%, 61.67%, 66.67%, lethality is respectively 38.33%, 38.33%, 33.33% (Fig. 9 C).Result shows, the serpentgrass root powder that feed adds 5%, 15%, 25% is fed prodenia litura 5 instar larvae, serpentgrass root powder on the growth of prodenia litura and the impact of survival less than the impact of feeding from 1-4 instar larvae, major part larva all can be developed to pupa, adult, but lethality is apparently higher than control group.
2.2.6 serpentgrass root impact that prodenia litura 6 instar larvae is grown
Prodenia litura 6 instar larvae is fed after 3d, and the prodenia lituras of 3 process of control group, interpolation 5%, 15%, 25% serpentgrass root powder have been developed to larva in latter stage (Figure 10 A) in 6 ages; Feed after 8d, the pupation rate of prodenia litura is respectively 88.33%, 88.33%, 86.67%, 81.67% (Figure 10 B); Feed after 20d, the eclosion rate of prodenia litura is respectively 76.67%, 808.00%, 76.67%, 73.33% (Figure 10 C); Feed after 24d, the accumulative eclosion rate of prodenia litura is respectively 81.67%, 80.00%, 76.67%, 76.67%, and cumulative mortality is respectively 18.33%, 20.00%, 23.33%, 23.33% (Figure 10 D).From rate of development, viability, pupation rate, eclosion rate aspect, add between the process of 5%, 15%, 25% serpentgrass root powder and the control group not adding serpentgrass root and there is no notable difference, prodenia litura 6 instar larvae of feeding after feed adds different content serpentgrass root powder is described, on the growth of prodenia litura and the impact of survival not remarkable.
3. conclusion and discussion
3.1 serpentgrasses are on the impact of diamond-back moth
The serpentgrass root powder of 5%, 15% or 25% is added in diamond-back moth feed, feed from newly hatched larvae, dead larvae is in 1 age, 2 age grade sections, and fraction can be grown to 3 age grade sections dead, do not have diamond-back moth can be developed to 4 age grade sections, after the 10d that feeds, 100% is dead; Feed from 2 instar larvaes, most of larva dies from 2 ages, 3 age grade sections, and fraction larva can grow 4 age grade sections and dead, and after the 13d that feeds, 100% is dead; Feed from 3 instar larvaes, most of larva dies from 3 instar larvaes, and part larva can be developed to 4 age grade sections, and after the 11d that feeds, lethality is higher than 96%, and after the 11d that feeds, 100% diamond-back moth is dead; Feed from 4 instar larvaes, part larva died from for 4 initial stages in age, and part larva can be developed to latter stage in 4 ages, but did not have larva to be developed to pupa time, and after the 9d that feeds, diamond-back moth 100% is dead; Feed adds between the process of 5%, 15%, 25% serpentgrass root, and each growth ratio in the length of time of diamond-back moth, mortality difference are not remarkable.And in the feed not adding serpentgrass root powder, no matter feed from 1 age, 2 ages, 3 ages or 4 ages, diamond-back moth all can normal development, pupate and emergence, final eclosion rate reaches 81.67%, 79.42%, 96.67%, 71.67% respectively.Result shows, and serpentgrass root powder all produces significant impact to growing of each instar larvae of diamond-back moth with surviving, and indicates serpentgrass root and has the active and cytotoxicity of stronger Developing restraint to diamond-back moth.
3.2 serpentgrasses are on the impact of prodenia litura
The serpentgrass root powder of 5%, 15% or 25% is added in prodenia litura feed, feed from newly hatched larvae, dead larvae is in 1 age, 2 age grade sections, and part can be grown to 3 age grade sections dead, do not have prodenia litura can be developed to 4 age grade sections, after the 20d that feeds, 100% is dead; Feed from 2 instar larvaes, the larva of more than 90% can be developed to 3 age grade sections and dead, and lethality 100% after the 20d that feeds, does not have larva can be developed to 4 age grade sections; Feed from 3 instar larvaes, fraction larva died from for 4 length of times, and most of larva dies from 5 ages or 6 length of times, and after the 30d that feeds, lethality 100%, does not have larva to be developed to pupa time; Feed from 4 instar larvaes, whole larva all can be developed to for 5 length of times, and part prodenia litura slowly can be developed to pupa, even sprouts wings, but in rate of development, viability, be starkly lower than the control group not adding serpentgrass root powder; Feed from 5 instar larvaes, the rate of development of prodenia litura is not obvious with the control group difference of not adding serpentgrass root powder, and most of larva all can pupate, sprout wings, but lethality will apparently higher than than control group; Feed from 6 instar larvaes, most of Spodoptera litura larvae of adding 5%, 15%, 25% serpentgrass root powder and control group all can be developed to pupa, adult, in rate of development, viability, larval weight, pupation rate, eclosion rate, 3 do not have notable difference between processed group and control group, between 3 processed group.And in the feed not adding serpentgrass root powder, prodenia litura of feeding from 1 age, 2 ages, 3 ages, 4 ages, 5 ages or 6 instar larvaes, prodenia litura all can normal development, pupate and emergence, final eclosion rate all reaches more than 80%.Result shows, serpentgrass root powder has very strong growth inhibitory activity and cytotoxicity to prodenia litura 1-4 instar larvae, has certain insecticidal activity to 5 instar larvaes.
3.3 prospect
Diamond-back moth and prodenia litura are the two kinds of worldwide brassicaceous vegetable important pests causing harm serious, the frequent use of a large amount of chemical pesticide and operational lack of standardization in agricultural production, cause the pesticide resistance of diamond-back moth and prodenia litura to increase year by year, difficulty of prevention and cure strengthens increasingly.And serpentgrass is the main forage plant of host of Cordyceps sinensis bat, have higher nutritive value, be also the natural feed of medicinal plant, livestock simultaneously.Result shows herein, serpentgrass root all has stronger growth inhibitory activity and cytotoxicity to test insect diamond-back moth and prodenia litura 1-4 instar larvae, to prodenia litura 5 instar larvae, there is certain insecticidal activity, be safe pesticide plant, have a extensive future.
Claims (5)
1. the application of serpentgrass root in preparation diamond-back moth or prodenia litura insecticide.
2. application according to claim 1, is characterized in that, described insecticide is stomach insecticide.
3. diamond-back moth or a prodenia litura insecticide, is characterized in that, comprises diamond-back moth or prodenia litura feed, and serpentgrass root powder.
4. diamond-back moth according to claim 3 or prodenia litura insecticide, is characterized in that, described insecticide is stomach insecticide.
5. the diamond-back moth according to claim 3 or 4 or prodenia litura insecticide, is characterized in that, the content of described serpentgrass root powder is mass fraction 5 ~ 25%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510680995.5A CN105192003B (en) | 2015-10-19 | 2015-10-19 | Serpentgrass root is preparing diamondback moth and the application in prodenia litura insecticide |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510680995.5A CN105192003B (en) | 2015-10-19 | 2015-10-19 | Serpentgrass root is preparing diamondback moth and the application in prodenia litura insecticide |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105192003A true CN105192003A (en) | 2015-12-30 |
| CN105192003B CN105192003B (en) | 2017-11-21 |
Family
ID=54940285
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510680995.5A Expired - Fee Related CN105192003B (en) | 2015-10-19 | 2015-10-19 | Serpentgrass root is preparing diamondback moth and the application in prodenia litura insecticide |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105192003B (en) |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102187779A (en) * | 2011-03-26 | 2011-09-21 | 重庆市中药研究院 | Low-altitude cultivation method for polygonum viviparum |
| CN102417391A (en) * | 2011-09-07 | 2012-04-18 | 重庆市中药研究院 | Serpentgrass planting method and rhizome cultivation special fertilizer |
-
2015
- 2015-10-19 CN CN201510680995.5A patent/CN105192003B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102187779A (en) * | 2011-03-26 | 2011-09-21 | 重庆市中药研究院 | Low-altitude cultivation method for polygonum viviparum |
| CN102417391A (en) * | 2011-09-07 | 2012-04-18 | 重庆市中药研究院 | Serpentgrass planting method and rhizome cultivation special fertilizer |
Non-Patent Citations (2)
| Title |
|---|
| 张欣，龙瑞军，淡瑞芳，丁学智: "高寒草原3种蓼科植物酚类物质含量及存在状态分析", 《甘肃农业大学学报》 * |
| 李刚，沈慧敏，张新虎: "甘肃天祝高寒草原16种植物丙酮提取物对粘虫的杀虫活性", 《甘肃农业大学学报》 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105192003B (en) | 2017-11-21 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101228853B (en) | Artificial feeding and culture transfer of monochamus alternatus hope | |
| CN106689823B (en) | Breeding feed for harmonia axyridis larvae and preparation method thereof | |
| CN105309142B (en) | Sexual propagation method for gastrodia elata | |
| Ghosh et al. | Economical and environmental importance of mulberry: a review | |
| JP2018536394A (en) | Artificial feed for bat larvae and method for preparing the same | |
| CN104605205A (en) | Artificial feed for harmonia axyridis larvae and preparation method thereof | |
| CN104770339A (en) | Method for artificially breeding alesia discolor fabricius | |
| CN110353133A (en) | A kind of man-made feeds of Spodopterafrugiperda larva and its application | |
| CN103934255A (en) | Method for treating waste meat by hermetia illucens larvae | |
| CN110036984B (en) | Method for breeding hepialus armoricanus in low-cost scale | |
| CN103621822B (en) | Artificial feed for adult mylabris phalerata pallas and preparation method thereof | |
| CN108041353A (en) | A kind of dichocrocis punctiferalis man-made feeds and its preparation method and application | |
| CN102578432B (en) | Artificial feed preparation and simplified feeding method for Athelis lepigone larva | |
| CN108813114B (en) | Artificial feed for corn borer larvae as well as preparation method and application thereof | |
| Demetillo et al. | Effect of Cymbopogon citratus (lemon grass) crude leaf extracts on the developmental stages of Pomacea canaliculata (golden apple snail). | |
| KR100869323B1 (en) | Longicorns feed and artificial breeding method of longicorns thereof | |
| WO2015003546A1 (en) | Culture method for natural species rejuvenation of artificially bred cordyceps host insect | |
| CN107801876B (en) | Artificial feed for adult cantharis, and preparation method and application thereof | |
| CN105613379A (en) | Method for ecologically breeding grass carps | |
| CN1994241A (en) | American white moth artificial breeding and propagating method and artificial feed for larva thereof | |
| CN105192003A (en) | Application of polygonum viviparum root in preparing insecticide for plutella xylostella and spodoptera litura fabricius | |
| Khaghaninia et al. | Investigation on biology of olive leaf worm Palpita unionalis Hb (Lepidoptera: Pyralidae) in constant laboratory conditions | |
| CN101779681B (en) | Insect repellant containing extract of witloof ethyl acetate and preparation thereof | |
| Ramos et al. | Spacings between plants with chicken manure in Roselle crop | |
| Sharma et al. | Standardization of Rearing Technique for Neoseiulus (= Amblyseius) longispinosus, a Predator of two Spotted Spider Mite |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| CB02 | Change of applicant information |
Address after: 510260 Xingang West Road, Guangdong, Guangzhou, No. 105, No. Applicant after: GUANGDONG INSTITUTE OF APPLIED BIOLOGICAL RESOURCES Address before: 510260 Xingang West Road, Guangdong, Guangzhou, No. 105, No. Applicant before: Guangdong Entomological Institute |
|
| COR | Change of bibliographic data | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CP01 | Change in the name or title of a patent holder | ||
| CP01 | Change in the name or title of a patent holder |
Address after: 510260 No. 105 West Xingang Road, Guangzhou, Guangdong, Haizhuqu District Patentee after: Institute of zoology, Guangdong Academy of Sciences Address before: 510260 No. 105 West Xingang Road, Guangzhou, Guangdong, Haizhuqu District Patentee before: Guangdong Institute of Applied Biological Resources |
|
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20171121 Termination date: 20211019 |