CN105192003B - Serpentgrass root is preparing diamondback moth and the application in prodenia litura insecticide - Google Patents
Serpentgrass root is preparing diamondback moth and the application in prodenia litura insecticide Download PDFInfo
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Abstract
The invention discloses a kind of serpentgrass root to prepare diamondback moth and the application in prodenia litura insecticide.The present invention is confirmed by testing, serpentgrass root has stronger growth inhibitory activity and cytotoxicity to 14 larvas of diamondback moth and prodenia litura, therefore serpentgrass root can be in the application in preparing diamondback moth or prodenia litura insecticide, so as to provide new resources for the exploitation of plant insecticide.
Description
Technical field：
The invention belongs to pesticide field, and in particular to serpentgrass root is preparing diamondback moth and answering in prodenia litura insecticide
With.
Background technology：
Diamondback moth Plutella xylostella (L.), prodenia litura Spodoptera litura Fabricius are generation
The important pests of criticality brassicaceous vegetable, fertility is strong, and generation overlap is serious, and the resistance to the action of a drug is strong, difficulty of prevention and cure height (Ahmad
Et al., 2008；Feng Xia etc., 2011；Zhang et al.,2015).
Serpentgrass (Polygonum viviparum L.) is polygonaceae Polygonum perennial herb, in China Tibet, Qinghai, Ji
Woods, the Inner Mongol, Shaanxi, Gansu, Sichuan etc. are distributed, and are mainly grown in 2220~5100m of height above sea level alpine steppe damply
Area (Li Youzhong etc., 1996).Serpentgrass is not only traditional Tibetan medicine, herbage and liquor-making raw material, and its rhizome is also the rare medicinal material in China
Host of Cordyceps sinensis insect main foodstuff (Li Youzhong etc., 1996；Wang Junli etc., 2010；Weng Hua, 2011；Shandong is added lustre,
2013)。
The content of the invention：
It is an object of the invention to provide application of the serpentgrass root in diamondback moth or prodenia litura insecticide is prepared.
Fresh serpentgrass of the present invention respectively at addition 5%, 15%, 25% in the man-made feeds of diamondback moth and prodenia litura
Root powder feeds diamondback moth and prodenia litura, determines influence of the fresh serpentgrass root to diamondback moth and Development of Spodoptera litura F,
As a result showing, serpentgrass root has very strong growth inhibitory activity and cytotoxicity to the 1-4 larvas of diamondback moth and prodenia litura,
There is obvious cytotoxicity to the instar larvae of prodenia litura 5.
Therefore, serpentgrass root can be applied in diamondback moth or prodenia litura insecticide is prepared.
Described insecticide is preferably stomach insecticide.
Another object of the present invention is to provide a kind of diamondback moth or prodenia litura insecticide, it is characterised in that including
Diamondback moth or prodenia litura feed, and serpentgrass root powder, the content of described serpentgrass root powder for mass fraction 5~
25%.
The present invention confirms that serpentgrass root has very strong growth to the 1-4 larvas of diamondback moth and prodenia litura by testing
Inhibitory activity and cytotoxicity, have obvious cytotoxicity to the instar larvae of prodenia litura 5, thus serpentgrass root can prepare it is small
Application in diamond-back moth or prodenia litura insecticide, so as to provide new resources and path for the exploitation of plant insecticide.
Brief description of the drawings：
Fig. 1 is the percentage that the instar larvae feeding 3d (A) of diamondback moth 1,8d (B) respectively develop age afterwards, is noted：Identical column-shape strip figure
On difference letter represent significant difference to each other；
Fig. 2 is the percentage that the instar larvae feeding 3d (A) of diamondback moth 2 and 9d (B) respectively develops age afterwards, is noted：Identical column-shape strip
Difference letter on figure represents significant difference to each other；
Fig. 3 is the percentage that the instar larvae feeding 3d (A) of diamondback moth 3 and 7d (B) respectively develops age afterwards, is noted：Identical column-shape strip
Difference letter on figure represents significant difference to each other；
Fig. 4 is the percentage that the instar larvae feeding 3d (A) of diamondback moth 4 and 6d (B) respectively develops age afterwards, is noted：Identical column-shape strip
Difference letter on figure represents significant difference to each other；
Fig. 5 is the percentage that the instar larvae feeding 9d (A) of prodenia litura 1,12d (B) respectively develop age with 20d (C) afterwards, is noted：
Difference letter on identical column-shape strip figure represents significant difference to each other；
Fig. 6 is the percentage that the instar larvae feeding 9d (A) of prodenia litura 2,12d (B) respectively develop age with 20d (C) afterwards, is noted：
Difference letter on identical column-shape strip figure represents significant difference to each other；
Fig. 7 is the percentage that the instar larvae feeding 8d (A) of prodenia litura 3,12d (B) respectively develop age with 20d (C) afterwards, is noted：
Difference letter on identical column-shape strip figure represents significant difference to each other；
Fig. 8 is the percentage that the instar larvae feeding 4d (A) of prodenia litura 4,12d (B), 28d (C) and 34d (D) respectively develop age afterwards
Than note：Difference letter on identical column-shape strip figure represents significant difference to each other；
Fig. 9 is the percentage that the instar larvae feeding 5d (A) of prodenia litura 5,12d (B) respectively develop age with 28d (C) afterwards, is noted：
Difference letter on identical column-shape strip figure represents significant difference to each other；
Figure 10 is the percentage that the instar larvae feeding 3d (A) of prodenia litura 6,8d (B), 20d (C) and 24d (D) respectively develop age afterwards
Than note：Difference letter on identical column-shape strip figure represents significant difference to each other.
Embodiment：
Following examples are to further explanation of the invention, rather than limitation of the present invention.
Embodiment 1：
1.1 for examination insect
Eggs of Diamondback Moth (P.xylostella) by Institute of Plant Protection of academy of agricultural sciences of Guangdong Province provide, prodenia litura (S.litura) ovum by
Zhongshan University Life Science College insect is provided.After the egg hatching of two kinds of insects, newly hatched larvae is taken to be used to raise as 1 instar larvae
Experiment is fed, diamondback moth newly hatched larvae is raised to 2,3,4 instar larvaes with diamondback moth man-made feeds is used for administering transgenic, prodenia litura
Newly hatched larvae is raised to 2,3,4,5,6 instar larvaes with prodenia litura man-made feeds is used for administering transgenic.
1.2 test plant
Serpentgrass root (P.viviparum) gathers from kangding, with aseptic water washing at least 3 after being cleaned up with clear water
It is secondary, after being placed in super-clean bench drying, package, 4 DEG C of preservations.Before preparing man-made feeds, remove the coring of serpentgrass root, cut fritter,
Then smashed with meat grinder as powder.
1.3 man-made feeds
The man-made feeds of diamondback moth are purchased from Institute of Plant Protection of academy of agricultural sciences of Guangdong Province, and the man-made feeds of prodenia litura are purchased from Zhongshan University
Life Science College insect institute.
1.4.1 the feeding measure of diamondback moth
1.4 feeding measure
The serpentgrass root powder of 5%, 15%, 25% (w/w) content is added in the man-made feeds of diamondback moth respectively, it is fully mixed
It is even, the feed that serpentgrass root powder content is 5%, 15%, 25% is prepared into, does not add the man-made feeds conduct pair of serpentgrass root powder
According to.The culture dish (diameter 9cm) for being lined with 2 layers of filter paper is taken, the fritter of feed 1 is added toward culture dish, 20 diamondback moth childrens is accessed per ware
Worm, often processing are repeated 3 times, and often handle total larva number 60.By culture dish be placed in temperature be 25 DEG C, relative humidity 70%, light
It is 14 according to the time:10(L:D climate box culture).Situations such as observing and record daily the feeding of larva, developing age, be dead,
Feed is changed in addition in 2-3 days.
1.4.2 the feeding measure of prodenia litura
The serpentgrass root powder of 5%, 15%, 25% (w/w) content is added in the man-made feeds of prodenia litura respectively, it is fully mixed
It is even, the feed that serpentgrass root powder content is 5%, 15%, 25% is prepared into, does not add the man-made feeds of serpentgrass root powder (0%)
As control.The plastic casing for being lined with 2 layers of filter paper is taken, feed 1-4 blocks are added toward plastic casing, the amount for adding feed regards the size of larva
Depending on, 20 Spodoptera litura larvaes are accessed per ware, often processing is repeated 3 times, and often handles total larva number 60.Culture dish is placed in temperature
Spend for 25 DEG C, relative humidity 70%, light application time 14:10(L:D climate box culture).Observation daily and record larva
Feeding, development age, it is dead situations such as, add feed daily.
1.5 data analysis
Each budding ratio of insect, the death rate represent that percentage is handled through evolution, arcsine transformation with percentage.With
SPSS16.0 softwares carry out one-way analysis of variance, and Multiple range test is examined using Duncan, level of signifiance P<0.05.
2 results and analysis
The influence that 2.1 serpentgrass roots grow to diamondback moth larvae
2.1.1 the influence that serpentgrass root grows to the instar larvae of diamondback moth 1
After the instar larvae of diamondback moth 1 feeding 3d, the pickles in the man-made feeds group (control group) of serpentgrass root powder are not added
Moth 8.33% is developed to 2 instar larvaes, and 83.33% is developed to 3 instar larvaes, 8.33% dead (Figure 1A)；And add 5%, 15%,
In 3 treatment groups of 25% serpentgrass root powder, the instar larvae ratio of diamondback moth 2 is respectively 48.33%, 60.00%, 48.33%, 3
Instar larvae ratio is respectively 6.67%, 5.00%, 1.67%, and the death rate is up to 45.00%, 35.00%, 50.00% respectively
(Figure 1A).After feeding 8d, 78.33% diamondback moth is developed to 4 instar larvaes in control group, and 8.3.% is pupated, and the death rate only has
13.33%；And add 5%, 15%, 25% serpentgrass root powder treatment group in the diamondback moth death rate respectively be up to 93.33%,
93.33%th, 88.33%, no diamondback moth can be developed to 4 instar larvaes (Figure 1B).Feed 14d after, in control group 81.67% it is small
Diamond-back moth has sprouted wings, and the death rate only has 15.00%；And add the diamondback moth in 5%, 15%, 25% serpentgrass root powder treatment group
100% is dead.As a result show, feed adds 5%, 15%, the 25% serpentgrass root powder feeding instar larvae of diamondback moth 1, development speed
Rate is substantially less than control group, and the death rate is significantly higher than control, adds between 3 treatment groups of serpentgrass root powder in developmental stage
Each age proportional difference unobvious, diamondback moth fail to be developed to for 4 ages and whole death.
2.1.2 the influence that serpentgrass root grows to the instar larvae of diamondback moth 2
After the instar larvae of diamondback moth 2 feeding 3d, the diamondback moth 88.99% in control group is developed to 3 instar larvaes, and 6.67% is dead
Die；And add in 3 treatment groups of 5%, 15%, 25% serpentgrass root powder, the instar larvae ratio of diamondback moth 2 is respectively 15.00%,
13.33%th, 6.67%, 3 instar larvae ratios are respectively 25.00%, 20.00%, 33.33%, the death rate is respectively 58.33%,
66.67%th, 60.00% (Fig. 2A).After feeding 9d, 77.75% diamondback moth has pupated in control group, and the death rate only has
14.78%；And the 3 instar larvae ratios added in the treatment group of 5%, 15%, 25% serpentgrass root powder be respectively 1.67%,
1.67%th, 3.33%, the death rate is respectively 98.33%, 98.33%, 96.67% (Fig. 2 B).After feeding 13d, in control group
79.42% diamondback moth has sprouted wings, and the death rate only has 20.58%；And add in 5%, 15%, 25% serpentgrass root powder treatment group
Diamondback moth 100% it is dead.As a result show, feed adds 5%, 15%, the 25% serpentgrass root powder feeding age of diamondback moth 2 children
Worm, developmental rate are substantially less than control group, and the death rate is significantly higher than control, add between 3 treatment groups of serpentgrass root powder
Each age proportional difference unobvious of developmental stage, diamondback moth all die from larval phase.
2.1.3 the influence that serpentgrass root grows to the instar larvae of diamondback moth 3
After the instar larvae of diamondback moth 3 feeding 3d, the diamondback moth 100% in control group is developed to 4 instar larvaes；And addition 5%,
15%th, in 3 treatment groups of 25% serpentgrass root powder, 3 instar larvae ratios are respectively 95.00%, 98.33%, 100%, dead
Rate is respectively 5.00%, 2.89%, 0.00% (Fig. 3 A).After feeding 7d, 100% diamondback moth has pupated in control group；And add
It is respectively 3.33%, 6.67%, 8.33%, 4 to add 3 instar larvae ratios in the treatment group of 5%, 15%, 25% serpentgrass root powder
Instar larvae ratio is respectively 8.33%, 8.33%, 11.67%, and the death rate is respectively 88.33%, 85.00%, 80.00% (figure
3B).After feeding 11d, 96.67% diamondback moth has sprouted wings in control group, and the death rate is 3.33%；And add 5%, 15%,
Diamondback moth 100% in 25% serpentgrass root powder treatment group is dead.As a result show, feed adds 5%, 15%, 25% bulbil
Knotweed root powder feeds the instar larvae of diamondback moth 3, and developmental rate is substantially less than control group, and the death rate is significantly higher than control, adds serpentgrass
Between 3 treatment groups of root powder larval phase is all died from each age proportional difference unobvious of developmental stage, diamondback moth.
2.1.4 the influence that serpentgrass root grows to the instar larvae of diamondback moth 4
After the instar larvae of diamondback moth 4 feeding 3d, the diamondback moth 71.67% in control group has been developed to latter stage larva in 4 ages,
15% pupates, and the death rate is 13.33%；And add in 3 treatment groups of 5%, 15%, 25% serpentgrass root powder, 4 initial stages in age
The ratio of larva is respectively 16.67%, 18.33%, 18.33%, in 4 ages the ratio of latter stage larva be respectively 30.00%,
26.67%th, 20.00%, the death rate is respectively 50.00%, 56.67%, 61.67% (Fig. 4 A).After feeding 6d, in control group
63.33% diamondback moth has pupated, and the ratio that the larva for handling latter stage larva in 4 ages accounts for is 11.67%, and the death rate is
25%,；And the ratio for adding diamondback moth 4 latter stage in the age larva in the treatment group of 5%, 15%, 25% serpentgrass root powder is respectively
11.67%th, 3.33%, 0%, it is 88.33%, 96.67%, 100% (Fig. 4 B) that the death rate is up to respectively.After feeding 9d, control
In group, 71.67% diamondback moth has sprouted wings, the death rate 28.33%；And add the processing of 5%, 15%, 25% serpentgrass root powder
Diamondback moth 100% in group is dead.As a result show, feed adds for 5%, 15%, the 25% serpentgrass root powder feeding age of diamondback moth 4
Larva, developmental rate are substantially less than control group, and the death rate is significantly higher than control, between 3 treatment groups for adding serpentgrass root powder
In each age proportional difference unobvious of developmental stage, diamondback moth all dies from larval phase.
The influence that 2.2 serpentgrass roots grow to Spodoptera litura larvae
2.2.1 the influence that serpentgrass root grows to the instar larvae of prodenia litura 1
After the instar larvae of prodenia litura 1 feeding 9d, in the control group for not adding serpentgrass root powder, 91.67% twill night
Moth is developed to 5 instar larvaes, the death rate 3.33%；And add the twill in 3 processing of 5%, 15%, 25% serpentgrass root powder
The instar larvae ratio of noctuid 2 is respectively 76.67%, 75.00%, 56.67%, the death rate is respectively 23.33%, 25.00%,
43.33%, no prodenia litura is developed to 3 ages (Fig. 5 A).After feeding 12d, 93.33% prodenia litura development in control group
To 6 instar larvaes, the death rate 6.67%；And add the prodenia litura 3 in 3 processing of 5%, 15%, 25% serpentgrass root powder
Instar larvae ratio is respectively 46.67%, 40.00%, 28.33%, and the death rate is respectively 53.33%, 60.00%, 71.67%
(Fig. 5 B).After feeding 20d, 20% prodenia litura is developed to 6 instar larvaes in control group, and 66.67% prodenia litura has pupated,
The death rate 13.33%；And the prodenia litura equal 100% added in 3 processing of 5%, 15%, 25% serpentgrass root powder is dead
(Fig. 5 C).86.67% pupates after prodenia litura feeding 24d in control group, and after feeding 32d, it is 86.67% to add up eclosion rate,
Cumulative mortality is 13.33%.As a result show, feed adds for 5%, 15%, the 25% serpentgrass root powder feeding age of prodenia litura 1
Larva, developmental rate are substantially less than control group, and the death rate is significantly higher than control, prodenia litura larva below 4 ages, without oblique
Autographa spp was developed to for 4 ages.
The influence that 4.2 serpentgrass roots grow to the instar larvae of prodenia litura 2
After the instar larvae of prodenia litura 2 feeding 9d, in the control group for not adding serpentgrass root powder, 100% prodenia litura
It is developed to 5 instar larvaes；And add the instar larvae ratio point of prodenia litura 3 in 3 processing of 5%, 15%, 25% serpentgrass root powder
Wei not be 90.00%, 96.67%, 96.67%, the death rate is respectively 10.00%, 3.33%, 3.33% (Fig. 6 A).Feed 12d
Afterwards, 100% prodenia litura is developed to 6 instar larvaes in control group；And add at 3 of 5%, 15%, 25% serpentgrass root powder
The instar larvae ratio of prodenia litura 3 in reason is respectively 23.33%, 21.67%, 25%, the death rate is respectively 76.67%,
78.33%th, 75% (Fig. 6 B).After feeding 20d, 91.67% prodenia litura is developed to pupa, the death rate 3.33% in control group；
And add the prodenia litura equal 100% dead (Fig. 6 C) in 3 processing of 5%, 15%, 25% serpentgrass root powder.In control group
Prodenia litura feeding 32d after, add up eclosion rate be 91.67%, cumulative mortality 3.33%.As a result show, feed addition
5%th, 15%, the 25% serpentgrass root powder feeding instar larvae of prodenia litura 2, developmental rate are substantially less than control group, and the death rate shows
Write and die from below 4 ages larva higher than control, prodenia litura, no prodenia litura was developed to for 4 ages.
2.2.3 the influence that serpentgrass root grows to the instar larvae of prodenia litura 3
After the instar larvae of prodenia litura 3 feeding 8d, in the control group for not adding serpentgrass root powder, 15% prodenia litura hair
Educate to 5 instar larvaes, 83.33% prodenia litura and be developed to 6 instar larvaes, the death rate 1.67%；And add 5%, 15%, 25%
The instar larvae ratio of prodenia litura 4 in 3 processing of serpentgrass root powder is respectively 6.67%, 10%, 15%, 5 instar larvae ratios
Respectively 56.67%, 70%, 73.33%, the death rate is respectively 36.67%, 20%, 13.33%, can be sent out without prodenia litura
Educate to 6 ages (Fig. 7 A).
After feeding 12d, 95% prodenia litura is developed to 6 instar larvaes in control group, and the death rate only has 1.67%；And add
Add 3 of 5%, 15%, 25% serpentgrass root powder handle in the instar larvae ratio of prodenia litura 5 be respectively 46.67%,
61.67%th, 78.33%, the death rate is respectively 53.33%, 38.33%, 21.67%, and no prodenia litura was developed to for 6 ages
(Fig. 7 B).After feeding 20d, 91.67% prodenia litura is developed to pupa, the death rate 6.67% in control group；And addition 5%,
15%th, the instar larvae ratio of prodenia litura 5 in 3 processing of 25% serpentgrass root powder is respectively 5%, 6.67%, 6.67%, 6
Age primary larva ratio is respectively 23.33%, 36.67%, 55%, and the death rate is respectively 71.67%, 56.67%, 38.33% (figure
7C).After having fed 30d, it is 88.33% that the prodenia litura in control group, which adds up eclosion rate, cumulative mortality 11.67%；And
The prodenia litura equal 100% added in 3 processing of 5%, 15%, 25% serpentgrass root powder is dead.As a result show, feed addition
5%th, 15%, the 25% serpentgrass root powder feeding instar larvae of prodenia litura 3, developmental rate are substantially less than control group, and the death rate shows
Write higher than control, for prodenia litura in dying from larval phase, no prodenia litura is developed to pupa time.
2.2.4 the influence that serpentgrass root grows to the instar larvae of prodenia litura 4
After the instar larvae of prodenia litura 4 feeding 4d, in the control group for not adding serpentgrass root powder, 46.67% twill night
Moth is developed to 5 instar larvaes, and 53.33% prodenia litura is developed to 6 instar larvaes；And add 5%, 15%, 25% serpentgrass root powder
3 processing in the instar larvae ratio of prodenia litura 5 be respectively 96.67%, 95%, 96.67%, 6 instar larvae ratios are respectively
3.33%th, 5%, 3.33% (Fig. 8 A).After feeding 12d, 13.33% prodenia litura is developed to 6 instar larvaes in control group,
83.33% prodenia litura has pupated, the death rate 3.33%；And add in 3 processing of 5%, 15%, 25% serpentgrass root powder
The instar larvae ratio of prodenia litura 6 is respectively 63.33%, 90%, 91.67%, and the death rate is respectively 35%, 10%, 8.33% (figure
8B).After feeding 28d, 86.67% prodenia litura sprouts wings in control group, and the death rate only has 13.33%；And addition 5%,
15%th, the prodenia litura adult in 3 processing of 25% serpentgrass root powder is respectively 13.33%, 3.33%, 6.67%, dead
Rate is
46.67%th, 56.67%, 55% (Fig. 8 C).After having fed 34d, the 3 of 5%, 15%, 25% serpentgrass root powder of addition
Prodenia litura adult ratio in individual processing is respectively 35.00%, 30.00%, 26.67%, hence it is evident that less than control group；The death rate
For 65.00%, 70.00%, 73.33% (Fig. 8 D).As a result show, the serpentgrass root powder that feed adds 5%, 15%, 25% is raised
The instar larvae of prodenia litura 4 is fed, developmental rate is substantially less than control group, and the death rate is significantly higher than control.
2.2.5 the influence that serpentgrass root grows to the instar larvae of prodenia litura 5
After the instar larvae of prodenia litura 5 feeding 4d, in the control group for not adding serpentgrass root powder, 98.33% twill night
Moth is developed to 6 instar larvaes, and the death rate only has 1.67%；And add oblique in 3 processing of 5%, 15%, 25% serpentgrass root powder
The prodenia litura of Autographa spp 71.67%, 76.67%, 85.00% is developed to 6 instar larvaes, death rate difference 28.33%,
23.33%th, 15.00% (Fig. 9 A).After feeding 12d, the prodenia lituraization of 13.33% prodenia litura 91.67% in control group
Pupa, the death rate only have 6.67%；And add the prodenia litura in 3 processing of 5%, 15%, 25% serpentgrass root powder
68.33%th, 68.33%, 73.33% prodenia litura pupates, and the death rate is increased to 31.67%, 31.67%, 26.67% respectively
(Fig. 9 B).After feeding 28d, 86.67% prodenia litura sprouts wings in control group, and the death rate only has 13.33%；And addition 5%,
15%th, the prodenia litura emergence ratio in 3 of 25% serpentgrass root powder processing be respectively 61.67%, 61.67%,
66.67%, the death rate is respectively 38.33%, 38.33%, 33.33% (Fig. 9 C).As a result show, feed addition 5%, 15%,
The 25% serpentgrass root powder feeding instar larvae of prodenia litura 5, the influence ratio of development and survival of the serpentgrass root powder to prodenia litura
The influence fed since 1-4 instar larvaes is small, and most of larva can be developed to pupa, adult, but the death rate is apparently higher than control
Group.
2.2.6 the influence that serpentgrass root grows to the instar larvae of prodenia litura 6
After the instar larvae of prodenia litura 6 feeding 3d, control group, 3 of 5%, 15%, 25% serpentgrass root powder processing are added
Prodenia litura has been developed to latter stage larva in 6 ages (Figure 10 A)；After feeding 8d, the percentage of pupation of prodenia litura is respectively 88.33%,
88.33%th, 86.67%, 81.67% (Figure 10 B)；After feeding 20d, the eclosion rate of prodenia litura is respectively 76.67%,
808.00%th, 76.67%, 73.33% (Figure 10 C)；After feeding 24d, the accumulative eclosion rate of prodenia litura is respectively 81.67%,
80.00%th, 76.67%, 76.67%, cumulative mortality is respectively 18.33%, 20.00%, 23.33%, 23.33% (figure
10D).From the aspect of rate of development, viability, percentage of pupation, eclosion rate, the processing of 5%, 15%, 25% serpentgrass root powder is added
There is no notable difference between the control group of no addition serpentgrass root, raised after illustrating feed addition different content serpentgrass root powder
The instar larvae of prodenia litura 6 is fed, the development to prodenia litura and the influence survived be not notable.
3. conclusion is with discussing
Influence of 3.1 serpentgrasses to diamondback moth
The serpentgrass root powder of addition 5%, 15% or 25%, feeds, larva is dead since newly hatched larvae in diamondback moth feed
Die in 1 age, 2 age grade sections, fraction can develop to 3 age grade sections and it is dead, no diamondback moth can be developed to 4 age grade sections, feed 10d
100% is dead afterwards；Fed since 2 instar larvaes, most of larva dies from 2 ages, 3 age grade sections, and fraction larva can develop 4 age grades
Section and it is dead, feed 13d after 100% death；Fed since 3 instar larvaes, most of larva dies from 3 instar larvaes, part larva
4 age grade sections can be developed to, after feeding 11d, the death rate is higher than 96%, and 100% diamondback moth is dead after feeding 11d；Opened from 4 instar larvaes
Beginning to feed, part larva died from for 4 initial stages in age, and part larva can be developed to latter stage in 4 ages, but no larva can be developed to pupa time,
After feeding 9d, diamondback moth 100% is dead；Between feed adds the processing of 5%, 15%, 25% serpentgrass root, each hair of diamondback moth
Child-bearing period ratio, mortality difference be not notable.And in the feed of no addition serpentgrass root powder, no matter from 1 age, 2 ages, 3 ages
Or 4 age start to feed, diamondback moth can normal development, pupate and sprout wings, final eclosion rate respectively up to 81.67%,
79.42%th, 96.67%, 71.67%.As a result show, serpentgrass root powder is equal with survival to growing for each instar larvae of diamondback moth
Significant impact is produced, indicate serpentgrass root has stronger suppression growth activity and cytotoxicity to diamondback moth.
Influence of 3.2 serpentgrasses to prodenia litura
The serpentgrass root powder of addition 5%, 15% or 25%, feeds, larva since newly hatched larvae in prodenia litura feed
It is dead in 1 age, 2 age grade sections, part can develop to 3 age grade sections and it is dead, no prodenia litura can be developed to 4 age grade sections, feed
100% is dead after 20d；Fed since 2 instar larvaes, more than 90% larva can be developed to 3 age grade sections and dead, feeding 20d
The death rate 100% afterwards, no larva can be developed to 4 age grade sections；Being fed since 3 instar larvaes, fraction larva died from for 4 ages,
Most of larva dies from 5 ages or 6 ages, and after feeding 30d, the death rate 100%, no larva can be developed to pupa time；From 4 instar larvaes
Start to feed, whole larvas can be developed to for 5 ages, and part prodenia litura can slowly be developed to pupa, or even sprout wings, but develop
The control group without addition serpentgrass root powder is significantly lower than in speed, viability；Fed since 5 instar larvaes, prodenia litura
Rate of development and the control group difference unobvious without addition serpentgrass root powder, most of larva can pupate, sprout wings, but dead
Rate will be apparently higher than than control group；Fed since 6 instar larvaes, add 5%, 15%, 25% serpentgrass root powder and control group
Most of Spodoptera litura larvae can be developed to pupa, adult, in rate of development, viability, larval weight, percentage of pupation, eclosion rate
Aspect, 3 do not have notable difference between treatment group and control group, between 3 treatment groups.And in no addition serpentgrass root powder
In feed, prodenia litura is fed since 1 age, 2 ages, 3 ages, 4 ages, 5 ages or 6 instar larvaes, prodenia litura can normal development, change
Pupa is with sprouting wings, and final eclosion rate is all up to more than 80%.As a result show, serpentgrass root powder has to prodenia litura 1-4 instar larvaes
Very strong growth inhibitory activity and cytotoxicity, there is certain insecticidal activity to 5 instar larvaes.
3.3 prospect
Diamondback moth and prodenia litura are the worldwide brassicaceous vegetable important pests of serious two kinds that cause harm, in agricultural production
A large amount of chemical pesticides it is frequent using and it is operational lack of standardization, cause the resistance to the action of a drug of diamondback moth and prodenia litura to increase year by year,
Difficulty of prevention and cure increases increasingly.And serpentgrass is the main forage plant of host of Cordyceps sinensis bat, there is higher nutritive value, together
When be also medicinal plant, the natural feed of livestock.This paper results show that serpentgrass root is to test insect diamondback moth and prodenia litura
1-4 instar larvaes are respectively provided with stronger growth inhibitory activity and cytotoxicity, have certain desinsection to the instar larvae of prodenia litura 5
Activity, it is safe pesticide plant, has a extensive future.
Claims (3)
1. application of the serpentgrass root in diamondback moth or prodenia litura insecticide is prepared, it is characterised in that described insecticide is
Stomach insecticide.
2. a kind of diamondback moth or prodenia litura insecticide, it is characterised in that including diamondback moth or prodenia litura feed, and serpentgrass
Root powder, described insecticide are stomach insecticide.
3. diamondback moth according to claim 2 or prodenia litura insecticide, it is characterised in that described serpentgrass root powder
Content be mass fraction 5~25%.
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102187779A (en) * | 2011-03-26 | 2011-09-21 | 重庆市中药研究院 | Low-altitude cultivation method for polygonum viviparum |
| CN102417391A (en) * | 2011-09-07 | 2012-04-18 | 重庆市中药研究院 | Serpentgrass planting method and rhizome cultivation special fertilizer |
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2015
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102187779A (en) * | 2011-03-26 | 2011-09-21 | 重庆市中药研究院 | Low-altitude cultivation method for polygonum viviparum |
| CN102417391A (en) * | 2011-09-07 | 2012-04-18 | 重庆市中药研究院 | Serpentgrass planting method and rhizome cultivation special fertilizer |
Non-Patent Citations (2)
| Title |
|---|
| 甘肃天祝高寒草原16种植物丙酮提取物对粘虫的杀虫活性;李刚，沈慧敏，张新虎;《甘肃农业大学学报》;20090415;第44卷(第2期);101-104 * |
| 高寒草原3种蓼科植物酚类物质含量及存在状态分析;张欣，龙瑞军，淡瑞芳，丁学智;《甘肃农业大学学报》;20080215;第43卷(第1期);126-129 * |
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